CN217781145U - Modular small-sized PCR reaction and detection device - Google Patents
Modular small-sized PCR reaction and detection device Download PDFInfo
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- CN217781145U CN217781145U CN202221579604.2U CN202221579604U CN217781145U CN 217781145 U CN217781145 U CN 217781145U CN 202221579604 U CN202221579604 U CN 202221579604U CN 217781145 U CN217781145 U CN 217781145U
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Abstract
The embodiment of the utility model provides a modularized small-sized PCR reaction and detection device, which comprises a turntable, a temperature control component, a driving component, a fluorescence detection component and a control module; the upper surface of the turntable is provided with a plurality of EP tube storage holes for storing EP tubes containing samples, and the temperature control assembly is used for heating the EP tubes; the fluorescence detection component can emit exciting light which is used for exciting a sample, and can also detect fluorescence emitted by the sample after being excited; the output end of the driving assembly is connected with the rotary table and is used for driving the rotary table to rotate, so that different EP tubes are rotated to the position below the fluorescence detection assembly; the control module can control the opening and closing of the driving assembly. The modularized small-sized PCR reaction and detection device has the advantages of simple structure, low production cost and high stability, and can realize the detection of samples at any time.
Description
Technical Field
The utility model relates to a PCR reaction and detection device technical field especially relate to a small-size PCR reaction of modularization and detection device.
Background
Polymerase Chain Reaction (PCR) is a reaction that uses a piece of DNA as a template and amplifies the piece of DNA to a sufficient amount for structural and functional analysis in the presence of both DNA polymerase and nucleotide substrates. The PCR detection method has great significance in the aspects of rapid diagnosis of bacterial infectious diseases and the like in clinical application.
Specifically, the temperature is increased to 93-96 ℃, hydrogen bonds of template double-stranded DNA are broken to form single strands, then the temperature is reduced to 50-55 ℃, so that primers are combined with the single-stranded DNA, then the temperature is increased to 60-75 ℃, and the primers combined with the single strands are polymerized into a DNA strand under the action of DNA polymerase, and the process is repeated, so that the exponential growth of the initial DNA strand can be realized.
In the PCR fluorescence detection, a sample is irradiated by excitation light, a dye or a probe in a DNA double strand is excited to generate fluorescence, and then the intensity of the fluorescence is detected by a photosensitive element, so that the concentration of target DNA in the sample is reflected. In the prior art, a 96-well plate is generally adopted as a sample carrier (such as CN 202111529964.1-a high-efficiency fluorescence quantitative PCR instrument), although the PCR fluorescence detector has the advantages of high flux and high stability, the whole cost is high, the PCR fluorescence detector is difficult to be applied to various scenes needing instant detection, and in addition, for the simultaneous detection requirements of a plurality of samples, such as the above-mentioned 96-well plate, although 96 samples can be detected simultaneously, in the stages of sample pretreatment and liquid preparation equipment, the samples need to be treated in batches once and then sealed together, and the samples can not be loaded into the instrument to perform a PCR fluorescence detection experiment when the samples are detected and the liquid preparation is completed.
SUMMERY OF THE UTILITY MODEL
The embodiment of the utility model provides a small-size PCR reaction of modularization and detection device for solve current PCR fluorescence detector cost of manufacture high and be difficult to be applied to the problem in the scene that various needs detected immediately.
In the embodiment of the present invention, the modular small-sized PCR reaction and detection device comprises a turntable, a temperature control assembly, a driving assembly, a fluorescence detection assembly and a control module;
the upper surface of the turntable is provided with a plurality of EP tube storage holes for storing EP tubes containing samples, and the temperature control assembly is used for heating the EP tubes; the fluorescence detection component can emit exciting light which is used for exciting a sample, and can also detect fluorescence emitted by the sample after being excited; the output end of the driving assembly is connected with the rotary table and is used for driving the rotary table to rotate, so that different EP tubes are rotated to the position below the fluorescence detection assembly; the control module can control the opening and closing of the driving component.
As a further alternative of the modular small PCR reaction and detection device, the fluorescence detection assembly includes a housing, a beam splitter, a filter, a light emitter, and an avalanche diode, the beam splitter and the filter being disposed in the housing;
the device comprises a shell, and is characterized in that the shell is provided with an entrance hole, an exit hole and a detection hole, a complete optical path can be formed between the entrance hole and the exit hole and between the exit hole and the detection hole, the avalanche diode is arranged in the detection hole, the illuminant is arranged in the entrance hole, the excitation light emitted by the illuminant is filtered by the filter plate, reflected by the spectroscope and enters the EP tube from the exit hole, and the fluorescence emitted by the sample in the EP tube after being excited sequentially passes through the exit hole and the spectroscope and then enters the avalanche diode through the detection hole.
As a further alternative of the modular compact PCR reaction and detection device, the detection holes are disposed on the top surface of the housing, the light-emitting holes are disposed on the bottom surface of the housing, the detection holes and the light-emitting holes form a vertical channel penetrating through the housing, the entrance holes are disposed on the side surface of the housing, the entrance holes penetrate through the housing from the side surface to the vertical channel, one of the beam splitters is disposed in the vertical channel and located on the light path of the excitation light of the incident Kong Sheru, and the beam splitter forms a certain angle with the horizontal plane, and the angle can reflect the excitation light into the light-emitting holes.
As a further alternative of the modular compact PCR reaction and detection device, the housing comprises an upper housing and a lower housing, the upper housing can be covered and connected with the lower housing to form the housing; the contact surface of the upper shell and the lower shell which are connected in a covering mode is an inclined plane, a groove is formed in the inclined plane, and the spectroscope is arranged in the groove and forms a certain angle with the horizontal plane.
As a further alternative of the modular small PCR reaction and detection device, a holding cavity is formed in the turntable, and the temperature control assembly is arranged in the holding cavity so as to heat the EP tube.
As a further alternative of the modular small PCR reaction and detection device, the modular small PCR reaction and detection device further comprises a thermal cover, the thermal cover is disposed on the upper surface of the turntable, the thermal cover is provided with a lofting opening and a light-passing hole, the light-passing hole is used for passing fluorescence emitted by a sample after being excited, and the lofting opening is used for placing the EP tube into the EP tube storage hole.
As a further alternative of the modular small PCR reaction and detection device, a distance is reserved between the fluorescence detection assembly and the thermal cover, a detection assembly shell is arranged on the outer side of the fluorescence detection assembly, the detection assembly shell comprises a light shielding plate and an upper cover, a cabin door is arranged on the upper cover, the upper cover is arranged above the fluorescence detection assembly, and the light shielding plate is enclosed between the upper cover and the thermal cover, so that a closed space is formed.
As a further alternative of the modular compact PCR reaction and detection device, the control module can control the hatch to be closed when the PCR reaction is in certain temperature intervals and to be opened when the PCR reaction is in other temperature intervals.
As a further alternative of the modular small PCR reaction and detection device, the device further comprises a base and a base housing, wherein the base housing encloses the outer side of the base to form an accommodating space with an upward opening, the driving assembly and the turntable are arranged in the accommodating space, and the base, the upper cover, the light shield and the base housing together form a closed structure.
As a further alternative of the modular small PCR reaction and detection device, the driving assembly comprises a motor and a coupler, a rotating shaft is arranged on the lower end face of the rotating disc, and the output end of the motor is connected with the rotating shaft through the coupler.
Implement the embodiment of the utility model provides a, will have following beneficial effect:
the EP tube is used for loading a sample, the EP tube is sealed, the step of sealing the sample film before detection can be omitted, the EP tube loaded with the sample is loaded by the turntable, the turntable rotates to switch the loading and detection positions, the whole mechanical structure is simple, the production cost is low, the detection module is fixed, and the stability is high; in general, the modular small-scale PCR reaction and detection device realizes the follow-up detection of samples.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Wherein:
FIG. 1 is a schematic diagram of an external structure of the modular small PCR reaction and detection device according to an embodiment of the present invention;
FIG. 2 is a schematic view of an assembly structure of the main components of the modular small PCR reaction and detection device according to an embodiment of the present invention;
FIG. 3 is an exploded view of a fluorescence detection assembly according to an embodiment of the present invention;
FIG. 4 is an exploded view of the modular compact PCR reaction and detection device according to an embodiment of the present invention;
description of the main element symbols:
10-rotating disc, 11-EP tube storage hole;
20-drive assembly, 21-motor, 22-coupling;
30-fluorescence detection component, 311-upper shell, 3111-detection hole, 312-lower shell, 3121-entrance hole, 32-spectroscope, 33-filter, 34-luminophor, 35-avalanche diode;
40-heat cover, 41-lofting opening, and 42-light through hole;
51-visor, 52-upper cover, 521-hatch;
61-base, 62-base housing;
70-EP tube.
Detailed Description
In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The embodiment of the utility model provides a small-size PCR reaction of modularization and detection device for solve current PCR fluorescence detector cost of manufacture high and be difficult to be applied to the problem in the scene that various needs detected immediately.
In the embodiment of the present invention, please refer to fig. 1 to fig. 3 in combination, the modular small PCR reaction and detection apparatus includes a turntable 10, a temperature control assembly, a driving assembly 20, a control module and a fluorescence detection assembly 30. Wherein, the upper surface of the turntable 10 is provided with a plurality of EP tube storage holes 11 for storing the EP tubes 70 containing the samples, and the temperature control component is used for heating the EP tubes 70; the fluorescence detection component 30 can emit excitation light for exciting the sample, and the fluorescence detection component 30 can also detect fluorescence emitted by the sample after being excited; the output end of the driving assembly 20 is connected to the turntable 10, and is used for driving the turntable 10 to rotate, so as to rotate different EP tubes 70 below the fluorescence detection assembly 30; the control module can control the opening and closing of the driving assembly 20.
During the use, the testing personnel put into EP pipe deposit hole 11 with EP pipe 70 in, temperature control assembly control EP pipe 70's heating process for template DNA in EP pipe 70 can circulate and carry out the three reaction steps of degeneration, annealing and extension, and when EP pipe 70 was in the annealing temperature interval, control module group was through controlling drive assembly 20 drive carousel 10 and is rotated, thereby rotates EP pipe 70 to the below of fluorescence detection subassembly 30 and detects.
The modular small PCR reaction and detection device utilizes the EP tube 70 to load a sample, the EP tube 70 is sealed, the step of film sealing on the sample before detection can be omitted, the EP tube 70 loaded with the sample is borne by the turntable 10, and the turntable 10 rotates to switch the sample loading and detection positions, so that the whole mechanical structure is simple, the production cost is low, the detection module is fixed, and the stability is high; in general, the modular small-scale PCR reaction and detection device realizes the follow-up detection of samples.
It is understood that the supply target of the modular small-scale PCR reaction and detection device is not limited to PCR, but is also applicable to various fluorescent probe method detection schemes (such as RCA, LAMP, etc.) of isothermal amplification systems.
In one embodiment, referring to fig. 4, the fluorescence detection assembly 30 includes a housing, a beam splitter 32, a filter 33, a light emitter 34, and an avalanche diode 35. The spectroscope 32 and the filter plate 33 are arranged in the shell; the shell is provided with an incident hole 3121, a light outlet hole (located on the bottom surface of the shell in the figure, not shown due to the visual angle limitation) and a detection hole 3111, a complete light path can be formed between the incident hole 3121 and the light outlet hole and between the light outlet hole and the detection hole 3111, the avalanche diode 35 is arranged in the detection hole 3111, the luminophor 34 is arranged in the incident hole 3121, exciting light emitted by the luminophor 34 is filtered by the filter 33, then reflected by the spectroscope 32 to enter the light outlet hole, and then enters the EP tube 70, and fluorescence emitted by a sample in the EP tube 70 after being excited sequentially passes through the light outlet hole and the spectroscope 32 and then enters the avalanche diode 35.
In the above embodiments, the filter 33 is used to filter the light emitted from the light emitter 34 to form the excitation light after filtering out certain wavelengths, and the beam splitter 32 mainly functions to change the optical path of the excitation light, so that the excitation light with different wavelengths can be obtained by changing the type and/or number of the filter 33, and different optical paths can be obtained by changing the number of the beam splitters 32 and the incident angles of the excitation light to the beam splitter 32, which should be considered as falling within the protection scope of the present application.
In one embodiment, the fluorescence detection assembly 30 is provided in a plurality to cover the emission spectrum of most commercially available fluorescent probes, thereby allowing detection of different sample types.
To simplify the structure of the fluorescence detection assembly 30, in a specific embodiment, the detection hole 3111 is disposed on the top surface of the housing, the light-emitting hole is disposed on the bottom surface of the housing, the detection hole 3111 and the light-emitting hole form a vertical channel penetrating through the housing, the incident hole 3121 is disposed on the side surface of the housing, the incident hole 3121 penetrates through the housing from the side surface to the vertical channel formed by the detection hole 3111 and the light-emitting hole, a beam splitter 32 is disposed in the vertical channel and is located on the light path of the excitation light emitted from the incident hole 3121, and the beam splitter 32 forms an angle with the horizontal plane, which can reflect the excitation light into the light-emitting hole.
In a more specific embodiment, the housing includes an upper housing 311 and a lower housing 312, the upper housing 311 can be connected to the lower housing 312 in a covering manner to form a complete housing; the contact surface of the upper shell 311 and the lower shell 312 is an inclined surface, a groove is arranged on the inclined surface of the upper shell 311 or the lower shell 312, and the spectroscope 32 is arranged in the groove so as to form a certain angle with the horizontal plane.
In the above embodiment, the detecting hole 3111 is formed in the upper housing 311, the light exit hole and the incident hole 3121 are formed in the lower housing 312, when the optical fiber bundle is mounted, the spectroscope 32 is placed in the groove of the upper housing 311 or the lower housing 312, the upper housing 311 and the lower housing 312 are locked by a fastener such as a bolt, the avalanche diode 35 is installed in the detecting hole 3111 of the upper housing 311, the filter 33 is placed in the incident hole 3121 of the lower housing 312, the light emitter 34 (which may be screwed or snapped) is installed, and one end of the light emitter 34 presses the filter 33.
In one embodiment, the modular small PCR reaction and detection apparatus further comprises a thermal cover 40, the thermal cover 40 covers the upper surface of the turntable 10, the thermal cover 40 is provided with a sample-placing opening 41 and a light-passing hole 42, the light-passing hole 42 is used for passing fluorescence emitted by the excited sample, and the sample-placing opening 41 is used for placing the EP tube 70 into the EP tube storage hole 11.
In a specific embodiment, a distance is left between the fluorescence detection assembly 30 and the thermal cover 40, a detection assembly housing is arranged outside the fluorescence detection assembly 30, the detection assembly housing includes a light shielding plate 51 and an upper cover 52, the upper cover 52 is arranged above the fluorescence detection assembly 30, and the light shielding plate 51 is enclosed between the upper cover 52 and the thermal cover 40, so as to form a closed space for isolating external stray light; the upper cover 52 is further provided with a hatch door 521, and a user can put in the EP tube 70 by opening the hatch door 521.
In a more specific embodiment, the control module can also control the door 521 to close when the PCR reaction is in certain temperature intervals and open when the PCR reaction is in other temperature intervals, and the temperature intervals that can be opened can be set according to the temperature intervals safe to the human body, so as to ensure that the user is not scalded when putting the EP tube 70, and the safety of the device is improved.
In a further specific embodiment, the modular compact PCR reaction and detection apparatus further includes a base 61 and a base housing 62, the base housing 62 encloses the outer side of the base 61, so as to form an upwardly open accommodating space, the driving assembly 20 and the turntable 10 are disposed in the accommodating space, and the base 61, the base housing 62, the light shielding plate 51 and the upper cover 52 together form a closed structure.
In one embodiment, the turntable 10 has a receiving cavity therein, and a temperature control assembly is disposed in the receiving cavity to heat the EP tube 70.
By adopting the scheme, the temperature control component can be closer to the EP pipe 70, and the heating effect is enhanced.
In a specific embodiment, the accommodating chamber is divided into a plurality of heating chambers, each heating chamber is provided with a temperature control assembly therein, and each temperature control assembly can work independently, so as to independently control the reaction stage of each EP tube 70.
In one embodiment, the EP tube storage well 11 is made of a material with a high reflectance or a coating of a material with a high reflectance is applied inside the EP tube storage well 11 to reflect more fluorescence to the avalanche diode 35.
In one embodiment, the driving assembly 20 includes a motor 21 and a coupling 22, a rotating shaft is disposed on the lower end surface of the rotating disc 10, and an output end of the motor 21 is connected to the rotating shaft through the coupling 22.
In a specific embodiment, the coupling 22 is locked with the output shaft of the motor 21 by a jackscrew.
In another specific embodiment, the shaft of the turntable 10 is bolted to the coupling 22.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the claims. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. A modularized small-sized PCR reaction and detection device is characterized by comprising a turntable, a temperature control component, a driving component, a fluorescence detection component and a control module;
the upper surface of the turntable is provided with a plurality of EP tube storage holes for storing EP tubes containing samples, and the temperature control assembly is used for heating the EP tubes; the fluorescence detection component can emit exciting light which is used for exciting a sample, and can also detect fluorescence emitted by the sample after being excited; the output end of the driving assembly is connected with the rotary table and is used for driving the rotary table to rotate, so that different EP tubes are rotated to the position below the fluorescence detection assembly; the control module can control the opening and closing of the driving assembly.
2. The modular compact PCR reaction and detection apparatus as claimed in claim 1, wherein the fluorescence detection assembly comprises a housing, a beam splitter, a filter, a light emitter, and an avalanche diode, the beam splitter and the filter being disposed in the housing;
the device comprises a shell, and is characterized in that the shell is provided with an entrance hole, an exit hole and a detection hole, a complete optical path can be formed between the entrance hole and the exit hole and between the exit hole and the detection hole, the avalanche diode is arranged in the detection hole, the illuminant is arranged in the entrance hole, the excitation light emitted by the illuminant is filtered by the filter plate, reflected by the spectroscope and enters the EP tube from the exit hole, and the fluorescence emitted by the sample in the EP tube after being excited sequentially passes through the exit hole and the spectroscope and then enters the avalanche diode through the detection hole.
3. The modular compact PCR reaction and detection device as claimed in claim 2, wherein the detection hole is disposed on the top surface of the housing, the light exit hole is disposed on the bottom surface of the housing, the detection hole and the light exit hole form a vertical channel penetrating the housing, the entrance hole is disposed on the side surface of the housing, the entrance hole penetrates the housing from the side surface to the vertical channel, one of the beam splitters is disposed in the vertical channel and is located on the optical path of the excitation light from the entrance Kong Sheru, and the beam splitter forms an angle with the horizontal plane, and the angle can reflect the excitation light into the light exit hole.
4. The modular compact PCR reaction and detection device according to claim 3, wherein the housing comprises an upper housing and a lower housing, the upper housing can be covered and connected with the lower housing to form the housing; the contact surface of the upper shell and the lower shell which are connected in a covering mode is an inclined surface, a groove is formed in the inclined surface, and the spectroscope is arranged in the groove and forms a certain angle with the horizontal plane.
5. The modular compact PCR reaction and detection apparatus of claim 1, wherein the turntable has a receiving cavity therein, and the temperature control assembly is disposed in the receiving cavity to heat the EP tube.
6. The modular compact PCR reaction and detection apparatus as claimed in claim 1, further comprising a thermal cover, wherein the thermal cover is disposed on the upper surface of the turntable, the thermal cover is provided with a sample-discharging opening and a light-transmitting hole, the light-transmitting hole is used for transmitting fluorescence emitted by the excited sample, and the sample-discharging opening is used for placing the EP tube into the EP tube storage hole.
7. The modular compact PCR reaction and detection apparatus of claim 6, wherein a distance is left between the fluorescence detection assembly and the thermal cover, a detection assembly housing is disposed outside the fluorescence detection assembly, the detection assembly housing comprises a light shielding plate and an upper cover, a hatch is disposed on the upper cover, the upper cover is disposed above the fluorescence detection assembly, and the light shielding plate is enclosed between the upper cover and the thermal cover, thereby forming a sealed space.
8. The modular compact PCR reaction and detection apparatus of claim 7, wherein the control module controls the door to be closed when the PCR reaction is in certain temperature intervals and to be opened when the PCR reaction is in other temperature intervals.
9. The modular compact PCR reaction and detection apparatus as claimed in claim 7, further comprising a base and a base housing, wherein the base housing encloses the outer side of the base to form an accommodation space opened upward, the driving assembly and the turntable are disposed in the accommodation space, and the base, the upper cover, the light shielding plate and the base housing together form a closed structure.
10. The modular small-scale PCR reaction and detection device according to claim 1, wherein the driving assembly comprises a motor and a shaft coupling, a rotating shaft is arranged on the lower end face of the rotating disc, and the output end of the motor is connected with the rotating shaft through the shaft coupling.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221579604.2U CN217781145U (en) | 2022-06-22 | 2022-06-22 | Modular small-sized PCR reaction and detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202221579604.2U CN217781145U (en) | 2022-06-22 | 2022-06-22 | Modular small-sized PCR reaction and detection device |
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| CN217781145U true CN217781145U (en) | 2022-11-11 |
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| CN202221579604.2U Active CN217781145U (en) | 2022-06-22 | 2022-06-22 | Modular small-sized PCR reaction and detection device |
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