CN217767030U - Slide glass for comet experiment - Google Patents

Abstract

The utility model discloses a slide glass for comet experiment belongs to medical instrument technical field. The upper surface of an experimental area (11) is provided with a frosting layer II, and a plurality of smooth experimental windows (12) which are arranged in an array manner are arranged in the experimental area (11); the cover plates I (2) are covered above the smooth experiment window (12) one by one; the cover plate II (3) covers all the smooth experiment windows (12) simultaneously, the anti-overflow groove I (16) is arranged around a single smooth experiment window (12), and the anti-overflow groove II (17) is arranged around all the smooth experiment windows (12). The utility model relates to a slide glass for comet experiment has solved the problem of coming unstuck in the gel solidification.

Description

Slide glass for comet experiment

Technical Field

The utility model relates to a slide glass for comet experiment belongs to medical instrument technical field.

Background

At present, glass slides on the market are generally smooth surfaces or frosted surfaces, the adhesion between a smooth glass slide and gel is insufficient, the glass slides are very easy to degum, and the frosted glass slide is not beneficial to microscope observation. Comet assay (comet assay) is a commonly used method for sensitive detection of DNA damage and repair at the single cell level. The slide glass for the comet assay commonly used at present is made of glass, the glass slide glass on the market at present is provided with a plurality of slotted holes, the slotted holes are formed by directly digging slotted holes 3 in the middle of a hydrophobic barrier 2 and attaching the slotted holes on a slide glass body 11, and the slide glass is covered with a cover glass when in use, as shown in figure 1. In the experimental process, the gel is laid in the slotted hole 3, and although the gel can be ensured not to fall off after being solidified, the gel is usually degummed in subsequent links such as cracking, unwinding and electrophoresis due to weak adhesion between the slide glass and the gel.

Disclosure of Invention

It shows to hold, the utility model aims to overcome the not enough of above-mentioned current slide glass, provide a slide glass for comet experiment to solve the phenomenon of coming unstuck.

The technical scheme of the utility model as follows:

the utility model relates to a slide glass for comet assay, including slide glass body and cover plate, the cover plate covers the top of slide glass body, the front of slide glass body is equipped with experimental area and recording area, the recording area sets up in the left side or the right side of experimental area, the upper surface of recording area is equipped with dull polish layer I;

the upper surface of the experimental area is provided with a frosting layer II, the roughness of the frosting layer II is larger than that of the frosting layer I, and a plurality of smooth experimental windows which are arranged in an array mode are arranged in the experimental area;

the cover plate comprises a cover plate I and a cover plate II,

the size of the cover plate I is larger than or equal to the longest size of 3/2 of the smooth experiment windows and smaller than or equal to the longest size of 2 of the smooth experiment windows, and the cover plates I cover the smooth experiment windows one to one;

and the cover plate II is rectangular, covers all the smooth experiment windows and exposes the recording area.

Optionally, the smooth experimental window is circular, elliptical, rectangular or profiled.

Optionally, the length of the coverslip ii = length of the slide body-width of the recording area.

Optionally, the back surface of the slide glass body is provided with a mark frame of the smooth experiment window, and the size of the smooth experiment window is smaller than that of the mark frame and smaller than that of the cover slip I.

Optionally, still include anti-overflow groove I, anti-overflow groove I sets up in the front of slide glass body, anti-overflow groove I sets up around single smooth experimental window, and the size of smooth experimental window < the size of anti-overflow groove I < the size of cover plate I.

Optionally, the depth of the overflow preventing groove is one third to one half of the thickness of the slide body.

Optionally, a color layer is arranged at the bottom of the anti-overflow groove.

Optionally, the slide glass further comprises an anti-overflow groove II, wherein the anti-overflow groove II is arranged around all the smooth experiment windows, and the depth of the anti-overflow groove II is one third to one half of the thickness of the slide glass body.

Optionally, the overflow preventing groove II is circular, oval, rectangular or special-shaped.

Advantageous effects

The utility model relates to a slide glass for comet experiment, through set up dull polish layer II in the experimental area of slide glass body, make the gel of laying overflow in the smooth experimental window accomodate by the dull polish clearance of smooth experimental window periphery, strengthened the cohesiveness of sample gel layer and smooth experimental window, the strong adhesion between gel area and the gel has solved the problem of coming unstuck in the gel solidification simultaneously; the overflow prevention groove I arranged around a single smooth experiment window and the overflow prevention grooves II arranged around all the smooth experiment windows also avoid the problem of cross contamination among samples caused by gel overflow.

Drawings

FIG. 1 is a schematic view of a conventional slide for comet assay;

fig. 2 is a schematic view of a first embodiment of the slide for a comet assay according to the present invention;

FIG. 3 isbase:Sub>A cross-sectional view A-A of FIG. 2;

FIG. 4 is a schematic view showing the use of the replaceable cover sheet II in FIG. 2;

FIG. 5 isbase:Sub>A cross-sectional view A-A of FIG. 4;

FIG. 6 is a schematic diagram of the added flag box of FIG. 2;

FIG. 7 isbase:Sub>A sectional view taken along line A-A of FIG. 6;

fig. 8 is a schematic view of a second embodiment of the slide for a comet assay according to the present invention;

FIG. 9 isbase:Sub>A cross-sectional view taken along line A-A of FIG. 8;

FIG. 10 is a schematic view of the usage state of FIG. 8;

fig. 11 is a schematic view of a third embodiment of the slide for comet assay of the present invention;

FIG. 12 isbase:Sub>A cross-sectional view taken along line A-A of FIG. 11;

FIG. 13 is a schematic view of the use state of FIG. 11;

wherein:

slide body 1

Experimental zone 11

Smooth experimental window 12

Recording area 14

Sign frame 15

Overflow-proof groove I16

Anti-overflow groove II 17

Cover plate I2

And a cover plate II 3.

Detailed Description

Example one

The utility model relates tobase:Sub>A slide glass for comet assay, slide glass include slide glass body 1 and cover plate, and the cover plate includes cover plate I2 and cover plate II 3, as shown in figure 2 and figure 3, figure 3 is the A-A cross-sectional view of figure 2. Generally, slide body 1 has a length of 76.2 mm, a width of 25.4 mm, and a thickness of 1 mm. The front surface of the slide body 1 is provided with an experimental area 11 and a recording area 14, the recording area 14 is arranged at the left side or the right side of the experimental area 11, and the recording area 14 is arranged at the left side of the experimental area 11 in the figure. The upper surface of the recording region 14 is provided with a frosted layer i for recording experimental information. The experimental area 11 is provided with a frosting layer II, a plurality of smooth experimental windows 12 which are arranged in an array are arranged in the experimental area, the smooth experimental windows 12 are round, oval, rectangular or special-shaped, and are smooth in the inner part, so that the smears can be spread smoothly by the gel. The roughness of the frosted layer ii of the experimental zone 11 is less than the roughness of the frosted layer i of the recording zone 14, as follows: the roughness of the frosted layer II of the experimental area 11 is 220 meshes, the roughness of the frosted layer I of the recording area 14 is 350 meshes, and the roughness value is larger and finer. The material of the slide glass body 1 can be quartz glass, high transparent polystyrene or other high polymer materials.

The shape of the cover sheet I2 is not limited, and can be circular, oval, rectangular or special-shaped, the size of the cover sheet I2 is larger than or equal to the longest size of 3/2 of the smooth experiment windows 12, and is smaller than or equal to the longest size of 2 smooth experiment windows 12, the cover sheet I2 is indicated by a circle in the figure, and the cover sheet I2 covers the smooth experiment windows 12 one by one. The thickness of the cover plate is typically 0.15 mm. The cover plate I2 is smooth and clean, and a comet experiment is carried out by a tabletting method when the cover plate is used. Specifically, the gel mixed solution is dripped into the smooth experiment window 12, then a cover plate I2 is covered above the smooth experiment window 12, certain pressure is applied to fully extend the gel, the thickness is moderate, the gel is spread to the area of the peripheral frosted layer II from the smooth experiment window 12, and finally a sample gel layer with a flat surface and the same shape with the cover plate I2 is formed. Lay the gel that overflows in the smooth experiment window 12 and be accomodate by the peripheral dull polish clearance of smooth experiment window 12, strengthened the adhesion of sample gel layer with smooth experiment window 12, the problem of coming unstuck in the gel solidification has been solved to the strong adhesive force between dull polish area and the sample gel layer simultaneously. After the gel layer of the sample in the smooth test window 12 was cured, the coverslip I2 was removed by gentle finger pressure.

The cover plate II 3 hasbase:Sub>A rectangular shape as shown in FIGS. 4 and 5, and FIG. 5 isbase:Sub>A sectional view taken along line A-A in FIG. 4. The length of cover slip ii 3 = the length of slide body 1-the width of recording area 14. In use, the cover II 3 covers all of the smooth test windows 12 simultaneously but exposes the recorded area 14 to facilitate identification of the recorded information. The cover plate II 3 is smooth and clean, an auxiliary gel layer covering all sample gels and the area of the peripheral frosted layer II is also manufactured by a tabletting method when the cover plate II is used, the sample gel layer can be protected by the integrally covered auxiliary gel, and meanwhile, the strong adhesive force formed by the auxiliary gel layer and the frosted area effectively solves the degumming problem in the subsequent experiment links such as cracking, unwinding, electrophoresis and the like.

The back of slide body 1 is provided withbase:Sub>A logo frame 15 of the smooth experimental window 12, as shown in fig. 6 and 7, and fig. 7 isbase:Sub>A cross-sectional viewbase:Sub>A-base:Sub>A of fig. 6. The size of the smooth test window 12 < the size of the logo box 15 < the size of the coverslip I2. The sign frame 15 may be colored, such as red, to facilitate the human eye to quickly identify the location of the smooth experimental window 12.

Example two

The utility model relates tobase:Sub>A slide glass for comet experiment, as shown in figure 8 and figures 9, 10, figure 9 is the A-A section view of figure 8. On the basis of the first embodiment, an anti-overflow groove I16 is added on the front surface of the slide glass body 1. The spill-resistant groove i 16 is disposed around a single smooth test window 12. The size of the smooth test window 12 < the size of the overflow prevention groove I16 < the size of the cover plate I2. Generally, the size of the no-spill well I16 should be much larger than the smooth test window 12 and slightly smaller than the size of the cover plate 12. The overflow prevention groove I16 is arranged to strengthen the adhesion between the gel and the slide body 1 and avoid cross contamination between samples caused by gel overflow. The depth of the overflow preventing groove 16 is one third to one half of the thickness of the slide body 1. The gel that spills over inside the smooth test window 12 is received by the frosted gap around the smooth test window 12 or further received by the anti-overflow groove i 16.

The bottom of the anti-overflow groove 16 may also be provided with a color layer, such as a red layer, to facilitate the human eye to quickly identify the position of the smooth experimental window 12.

EXAMPLE III

The utility model relates tobase:Sub>A slide glass for comet assay, as shown in fig. 11 and 12, 13, fig. 12 is the A-A section view of fig. 11. On the basis of the second embodiment, an anti-overflow groove II 17 is added on the front surface of the slide glass body 1. The anti-overflow groove II 17 is arranged around all the smooth experiment windows 12 and is circular, oval, rectangular or special-shaped. Generally, the size of the overflow preventing groove II 17 is slightly smaller than that of the cover plate II 3. The depth of anti-overflow groove II 17 is one third to one half of slide glass body 1's thickness, prevents that the gel from overflowing slide glass body 1, also helps consolidating the adhesive force between gel and slide glass body 1 simultaneously.

It should be noted that those skilled in the art can make modifications to the embodiments of the present invention without departing from the scope of the claims of the present invention. Accordingly, the scope of the claims of the present invention is not to be limited to the specific embodiments described above.

Claims (9)

1. A slide for comet experiments, which comprises a slide body (1) and a cover plate, wherein the cover plate is covered above the slide body (1),

the test paper carrier is characterized in that an experimental area (11) and a recording area (14) are arranged on the front side of the slide paper body (1), the recording area (14) is arranged on the left side or the right side of the experimental area (11), and a frosting layer I is arranged on the upper surface of the recording area (14);

a frosting layer II is arranged on the upper surface of the experimental area (11), the roughness of the frosting layer II is smaller than that of the frosting layer I, and a plurality of smooth experimental windows (12) which are arranged in an array mode are arranged in the experimental area (11);

the cover plate comprises a cover plate I (2) and a cover plate II (3),

the size of the cover plate I (2) is larger than or equal to the longest size of 3/2 of the smooth experiment windows (12) and smaller than or equal to the longest size of 2 of the smooth experiment windows (12), and the cover plates I (2) are covered above the smooth experiment windows (12) in a one-to-one mode;

the cover plate II (3) is rectangular, covers all the smooth experiment windows (12) at the same time, and exposes the recording area (14).

2. Slide for comet experiments according to claim 1, characterized in that the smooth experimental window (12) is circular, oval or rectangular.

3. Slide for comet experiments according to claim 1, wherein the length of the cover plate ii (3) = length of the slide body (1) -width of the recording area (14).

4. The slide for comet assay according to claim 1, wherein the back of the slide body (1) is provided with a sign frame (15) of the smooth assay window (12), and the size of the smooth assay window (12) < the size of the sign frame (15) < the size of the cover slip i (2).

5. The slide for comet assay according to any one of claims 1 to 4, further comprising an anti-overflow slot I (16), wherein the anti-overflow slot I (16) is disposed on the front face of the slide body (1), the anti-overflow slot I (16) is disposed around a single smooth assay window (12), and the size of the smooth assay window (12) < the size of the anti-overflow slot I (16) < the size of the cover plate I (2).

6. The slide for a comet assay according to claim 5 wherein the depth of the overflow preventing groove I (16) is one third to one half of the thickness of the slide body (1).

7. The slide for comet experiments according to claim 6, wherein the bottom of the overflow preventing groove I (16) is provided with a color layer.

8. The slide for comet experiments according to any one of claims 1 to 4, further comprising an overflow prevention groove II (17), wherein the overflow prevention groove II (17) is arranged around all the smooth experiment windows (12), and the depth of the overflow prevention groove II (17) is one third to one half of the thickness of the slide body (1).

9. The slide for comet experiments according to claim 8, wherein the overflow prevention groove II (17) is circular, elliptical or rectangular.

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