CN217766209U

CN217766209U - DNA electrophoresis imaging detection device

Info

Publication number: CN217766209U
Application number: CN202221178448.9U
Authority: CN
Inventors: 劳淑华; 毛旭丹; 谭小珊; 龙柳妃
Original assignee: Hangzhou Meiyi Biotechnology Co ltd
Current assignee: Hangzhou Meiyi Biotechnology Co ltd
Priority date: 2022-05-13
Filing date: 2022-05-13
Publication date: 2022-11-08
Anticipated expiration: 2032-05-13

Abstract

The utility model relates to the technical field of detection devices, and discloses a DNA electrophoresis imaging detection device, which comprises a U-shaped plate, a CCD image sensor and a light supplementing source, wherein a placing plate is horizontally arranged in the U-shaped plate, a plurality of placing holes which are uniformly distributed are formed in the upper surface of the placing plate, and a clamping mechanism is arranged in each placing hole; a lamp cavity is formed in the bottom of the placing plate, the light supplementing source is located inside the lamp cavity, and the placing hole is communicated with the lamp cavity; the bottom of the U-shaped plate is provided with a driving mechanism for driving the placing plate to move; the CCD image sensor is arranged at the top of the U-shaped plate. The utility model discloses can detect multiunit DNA sample, and be convenient for carry out the dismouting to the DNA sample, improve detection efficiency.

Description

DNA electrophoresis imaging detection device

Technical Field

The utility model relates to a detection device technical field especially relates to a DNA electrophoresis formation of image detection device.

Background

Capillary electrophoresis is an important separation detection technique. Capillary electrophoresis combined with sieving medium has good separation capability for biological macromolecules, such as DNA and protein, which is one of the important bases of modern life science research. With the continuous development of fields such as life sciences, the application demand of capillary electrophoresis is increasing. The application range is expanding from the simple academic research field to more practical fields.

The patent number is CN202159019U, and a detection device is disclosed, which comprises a capillary electrophoresis unit, a light source and a photoelectric detector, wherein the signal output end of the photoelectric detector is connected with a microprocessor system, the photoelectric detector comprises a CCD image sensor, and the signal output end of the CCD image sensor is connected with the microprocessor system. Also comprises a DNA sample at the starting point of the capillary electrophoresis unit, wherein the DNA sample contains a fluorescent substance.

In the scheme, imaging detection can be carried out on only one group of DNA samples, and when a plurality of groups of DNA samples need to be detected, the DNA samples need to be repeatedly moved up and down, so that the detection efficiency of the DNA samples is reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problem that the DNA electrophoresis formation of image detection device detection efficiency is low among the prior art, and the DNA electrophoresis formation of image detection device who proposes.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a DNA electrophoresis imaging detection device comprises a U-shaped plate, a CCD image sensor and a light supplementing source, wherein a placing plate is horizontally arranged inside the U-shaped plate, a plurality of uniformly distributed placing holes are formed in the upper surface of the placing plate, and a clamping mechanism is arranged inside the placing holes;

the bottom of the placing plate is provided with a lamp cavity, the light supplementing source is positioned in the lamp cavity, and the placing hole is communicated with the lamp cavity;

the bottom of the U-shaped plate is provided with a driving mechanism for driving the placing plate to move;

the CCD image sensor is arranged at the top of the U-shaped plate.

Preferably, fixture includes clamp splice, connecting rod, connecting block and spring, place the inside of board and be located the both sides of placing the hole all are equipped with the spring chamber, the connecting block is located the inside in spring chamber and pass through the inside wall fixed connection in spring and spring chamber, the connecting rod fix set up in on one side of spring is kept away from to the connecting block, and the other end of spring extend to place the inside in hole and with clamp splice fixed connection.

Preferably, the clamping block is a rubber block.

Preferably, actuating mechanism includes screw rod, screw thread piece and bracing piece, the bottom of U template is equipped with the drive chamber, the screw rod transversely rotate set up in the inside in drive chamber, and the one end of screw rod extends to the outside of U template is fixed and is equipped with the rocking handle, screw thread piece screw thread set up in on the pole wall of screw rod, the lower extreme of bracing piece fixed set up in the top of screw thread piece, and the upper end of bracing piece extend to the inside of U template and with place board fixed connection.

Preferably, a fixing rod is fixedly arranged at one end of the light supplementing source, the other end of the fixing rod extends to the outside of the U-shaped plate and is fixedly provided with a fixing plate, and a bolt in threaded connection with the side wall of the U-shaped plate is arranged on the side wall of the fixing plate.

Preferably, a positioning column is fixedly arranged on the side wall of the lamp cavity, and a positioning groove matched with the positioning column is formed in the side wall of the light supplementing source.

Preferably, the placing hole is internally and fixedly provided with a bearing plate, and the bearing plate is made of transparent glass.

Preferably, an L-shaped supporting plate is fixedly arranged at the top of the U-shaped plate, an electric cylinder is fixedly arranged at the top of the L-shaped supporting plate, and the CCD image sensor is fixedly arranged at the tail end of a piston rod of the electric cylinder.

Preferably, the CCD image sensor is located directly above the placing plate.

Compared with the prior art, the utility model provides a DNA electrophoresis formation of image detection device possesses following beneficial effect:

1. this DNA electrophoresis formation of image detection device, through the board of placing that is equipped with, and the surface of placing the board is equipped with a plurality of holes of placing, can place a plurality of DNA sample slide glasses, is equipped with clamp splice, connecting rod, connecting block and spring simultaneously in the inside of placing the hole, can improve the precision that detects with the firm centre gripping of slide glass in the inside of placing the hole.

2. This DNA electrophoresis formation of image detection device through setting up screw rod, screw thread piece, bracing piece and the rocking handle in U template bottom, rotates the rocking handle and makes the screw rod rotatory, can drive the screw thread piece when the screw rod is rotatory and remove to can drive and place the board and remove in the inside of U template, and then can detect a plurality of DNA samples, improve detection efficiency.

The part that does not relate to in the device all is the same with prior art or can adopt prior art to realize, the utility model discloses can detect multiunit DNA sample, and be convenient for carry out the dismouting to the DNA sample, improve detection efficiency.

Drawings

FIG. 1 is a schematic structural diagram of a DNA electrophoresis imaging detection device provided by the present invention;

FIG. 2 is an enlarged view of a portion A of FIG. 1;

FIG. 3 is an enlarged view of a portion B of FIG. 1;

fig. 4 is a perspective view of the placement plate of fig. 1.

In the figure: 1. a U-shaped plate; 2. a CCD image sensor; 3. a light supplement source; 4. placing the plate; 5. placing holes; 6. a clamping block; 7. a connecting rod; 8. connecting blocks; 9. a spring; 10. a screw; 11. a thread block; 12. a support bar; 13. a rocking handle; 14. a fixing rod; 15. a fixing plate; 16. a bolt; 17. an L-shaped support plate; 18. an electric cylinder; 19. a positioning column; 20. a carrier plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Example 1

Referring to fig. 1-2, a DNA electrophoresis imaging detection device, including U template 1, CCD image sensor 2 and light filling source 3,U template 1's inside level is equipped with places board 4, place the upper surface of board 4 and set up a plurality of evenly distributed's hole 5 of placing, the fixed loading board 20 that is equipped with in inside of placing hole 5, and loading board 20 adopts the transparent glass board, the inside of placing hole 5 is equipped with fixture, fixture includes clamp 6, connecting rod 7, connecting block 8 and spring 9, place the inside of board 4 and be located the both sides of placing hole 5 and all be equipped with the spring chamber, connecting block 8 is located the inside of spring chamber and through spring 9 and the inside wall fixed connection in spring chamber, connecting rod 7 is fixed to be set up on one side that spring 9 was kept away from to connecting block 8, and the other end of spring 9 extends to the inside of placing the hole and with clamp 6 fixed connection, clamp 6 adopts the rubber piece, the rubber piece can reduce the centre gripping wearing and tearing to the slide, promote clamp 6 to remove and put into between two rethread clamp 6.

Example 2

Referring to 1,U, a driving mechanism for driving the placing plate 4 to move is arranged at the bottom of the template 1, the driving mechanism includes a screw rod 10, a thread block 11 and a supporting rod 12, a driving cavity is arranged at the bottom of the U-shaped plate 1, the screw rod 10 is transversely rotatably arranged inside the driving cavity, one end of the screw rod 10 extends to the outside of the U-shaped plate 1 and is fixedly provided with a rocking handle 13, the thread block 11 is threadedly arranged on the rod wall of the screw rod 10, the lower end of the supporting rod 12 is fixedly arranged at the top of the thread block 11, the upper end of the supporting rod 12 extends to the inside of the U-shaped plate 1 and is fixedly connected with the placing plate 4, the rocking handle 13 is rotated to rotate the screw rod 10, the screw rod 10 can move the thread block 11 when rotating, the thread block 11 can drive the placing plate 4 to horizontally move in the inside of the U-shaped plate 1, so that a plurality of glass slides can sequentially move to the lower side of the CCD image sensor 2, and can display a DNA sample image on a display.

Example 3

Referring to fig. 1 and 3-4, a lamp cavity is arranged at the bottom of a placing plate 4, a light supplementing source 3 is positioned inside the lamp cavity, a placing hole 5 is communicated with the lamp cavity, a fixing rod 14 is fixedly arranged at one end of the light supplementing source 3, the other end of the fixing rod 14 extends to the outside of the U-shaped plate 1 and is fixedly provided with a fixing plate 15, and a bolt 16 in threaded connection with the side wall of the U-shaped plate 1 is arranged on the side wall of the fixing plate 15; the fixed reference column 19 that is equipped with of lateral wall in lamp chamber, light filling source 3's lateral wall offer with reference column 19 matched with constant head tank, unscrew bolt 16 and can take off fixed plate 15 from the lateral wall of placing board 4 to can take out light filling source 3 from the inside of placing board 4.

Example 4

Referring to fig. 1, the CCD image sensor 2 is arranged on the top of the U-shaped plate 1, the CCD image sensor 2 is located right above the placing plate 4, the top of the U-shaped plate 1 is fixedly provided with an L-shaped supporting plate 17, the top of the L-shaped supporting plate 17 is fixedly provided with an electric cylinder 18, the CCD image sensor 2 is fixedly arranged at the end of a piston rod of the electric cylinder 18, and a power supply of the electric cylinder 18 is switched on, so that the electric cylinder 18 extends out to adjust the height of the CCD image sensor 2.

The utility model discloses in, during the use, the staff puts into the inside of placing hole 5 with the slide glass earlier, spring 9 through placing hole 5 both sides gives connecting block 8 and connecting rod 7 elasticity of applying, make clamp splice 6 with the firm centre gripping of slide glass in the inside of placing hole 5, the bottom of placing hole 5 this moment is provided with light supplement source 3, can shine the sample on slide glass surface clearly, then rotate rocking handle 13 and make screw rod 10 rotatory, screw rod 10 can make thread block 11 remove when rotatory, thread block 11 can drive places board 4 at the inside horizontal migration of U template 1, thereby can a plurality of slide glass remove to CCD image sensor 2's below in proper order, can show on the display to DNA sample image.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The DNA electrophoresis imaging detection device comprises a U-shaped plate (1), a CCD image sensor (2) and a light supplement source (3), and is characterized in that a placing plate (4) is horizontally arranged inside the U-shaped plate (1), a plurality of uniformly distributed placing holes (5) are formed in the upper surface of the placing plate (4), and a clamping mechanism is arranged inside the placing holes (5);

a lamp cavity is formed in the bottom of the placing plate (4), the light supplementing source (3) is located in the lamp cavity, and the placing hole (5) is communicated with the lamp cavity;

the bottom of the U-shaped plate (1) is provided with a driving mechanism for driving the placing plate (4) to move;

the CCD image sensor (2) is arranged at the top of the U-shaped plate (1).

2. The DNA electrophoresis imaging detection device according to claim 1, wherein the clamping mechanism comprises a clamping block (6), a connecting rod (7), a connecting block (8) and a spring (9), a spring cavity is arranged inside the placing plate (4) and on both sides of the placing hole (5), the connecting block (8) is arranged inside the spring cavity and fixedly connected with the inner side wall of the spring cavity through the spring (9), the connecting rod (7) is fixedly arranged on one side of the connecting block (8) far away from the spring (9), and the other end of the spring (9) extends to the inside of the placing hole and is fixedly connected with the clamping block (6).

3. The DNA electrophoresis imaging detection device according to claim 2, wherein the clamping block (6) is a rubber block.

4. The DNA electrophoresis imaging detection device according to claim 1, wherein the driving mechanism comprises a screw (10), a thread block (11) and a support rod (12), a driving cavity is arranged at the bottom of the U-shaped plate (1), the screw (10) is transversely and rotatably arranged in the driving cavity, one end of the screw (10) extends to the outside of the U-shaped plate (1) and is fixedly provided with a rocking handle (13), the thread block (11) is threadedly arranged on the rod wall of the screw (10), the lower end of the support rod (12) is fixedly arranged at the top of the thread block (11), and the upper end of the support rod (12) extends to the inside of the U-shaped plate (1) and is fixedly connected with the placing plate (4).

5. The DNA electrophoresis imaging detection device as claimed in claim 1, wherein a fixing rod (14) is fixedly arranged at one end of the light supplement source (3), the other end of the fixing rod (14) extends to the outside of the U-shaped plate (1) and is fixedly provided with a fixing plate (15), and a bolt (16) in threaded connection with the side wall of the U-shaped plate (1) is arranged on the side wall of the fixing plate (15).

6. The DNA electrophoresis imaging detection device according to claim 1, wherein a positioning column (19) is fixedly arranged on the side wall of the lamp cavity, and a positioning groove matched with the positioning column (19) is formed on the side wall of the light supplement source (3).

7. The DNA electrophoresis imaging detection device according to claim 1, wherein a carrier plate (20) is fixed inside the placement hole (5), and the carrier plate (20) is a transparent glass plate.

8. The DNA electrophoresis imaging detection device according to claim 1, wherein an L-shaped support plate (17) is fixedly arranged on the top of the U-shaped plate (1), an electric cylinder (18) is fixedly arranged on the top of the L-shaped support plate (17), and the CCD image sensor (2) is fixedly arranged at the tail end of a piston rod of the electric cylinder (18).

9. The DNA electrophoresis imaging detection device according to claim 1, wherein the CCD image sensor (2) is located directly above the placing plate (4).