CN214703419U

CN214703419U - Quantitative high-precision capillary electrophoresis apparatus

Info

Publication number: CN214703419U
Application number: CN202120487658.5U
Authority: CN
Inventors: 褚延梁; 陈曈; 李昊阳; 路晓菲
Original assignee: Shandong Fangtansi Biomedical Industry Technology Research Institute Co ltd
Current assignee: Shandong Fangtansi Biomedical Industry Technology Research Institute Co ltd
Priority date: 2021-03-08
Filing date: 2021-03-08
Publication date: 2021-11-12
Anticipated expiration: 2031-03-08

Abstract

The utility model discloses a quantitative high-precision capillary electrophoresis apparatus, which comprises an installation base, wherein the top of the installation base is fixedly connected with a high-voltage power supply, an application of sample groove, a buffer groove, a fixed plate and a clamping mechanism, the top of the fixed plate is fixedly provided with a capillary, and the top of the fixed plate is provided with a fluorescence detector; the fixture includes that fixed connection is at the U-shaped mounting bracket at installation base top, the top of U-shaped mounting bracket is provided with the splint of two symmetries, the holding tank has been seted up at the top of U-shaped mounting bracket, be provided with two connecting blocks corresponding with two splint in the holding tank, two the holding tank is all run through to the bottom of connecting block, and the bottom fixedly connected with connecting plate of connecting block. The utility model discloses in, through fixture, can be equipped with the syringe centre gripping of sample solution with different models between two splint, after starting electric telescopic handle, can promote the syringe to the accurate quantitative application of sample article solution that adds in the application of sample groove.

Description

Quantitative high-precision capillary electrophoresis apparatus

Technical Field

The utility model relates to a capillary electrophoresis apparatus technical field especially relates to a quantitative high accuracy capillary electrophoresis apparatus.

Background

The capillary electrophoresis apparatus uses an elastic quartz capillary as a separation channel, uses a high-voltage direct-current electric field as a driving force, and realizes separation of each component according to the difference between mobility (migration speed under unit electric field intensity) and distribution behavior of each component in a sample. When the capillary electrophoresis column is loaded with electrolyte, electroosmotic flow can be generated due to existence of silicon hydroxyl on the tube wall, and the electroosmotic flow can push the solution in the whole capillary column to move directionally. The main difference from the general chromatographic technique is that the separation principle is not based on the partition coefficients of components in a mobile phase and a stationary phase, but on the difference of ion migration speeds under the action of an electric field.

Capillary electrophoresis appearance wide application has the separation of the protein mixture sample of different isoelectric points, and along with the development and the progress of society, capillary electrophoresis appearance is using more and more extensively, but there is certain defect in current capillary electrophoresis appearance structural design, but most people of current capillary electrophoresis appearance are for adding sample solution, can't realize accurate sample solution that adds, can cause sample solution's waste, for this reason, we have proposed a new quantitative high accuracy capillary electrophoresis appearance.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a quantitative high-precision capillary electrophoresis apparatus.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a quantitative high-precision capillary electrophoresis apparatus comprises an installation base, wherein the top of the installation base is fixedly connected with a high-voltage power supply, a sample adding groove, a buffer groove, a fixing plate and a clamping mechanism, a capillary is fixedly installed at the top of the fixing plate, and a fluorescence detector is arranged at the top of the fixing plate;

fixture includes the U-shaped mounting bracket of fixed connection at installation base top, the top of U-shaped mounting bracket is provided with the splint of two symmetries, the holding tank has been seted up at the top of U-shaped mounting bracket, be provided with in the holding tank with two splint corresponding two connecting blocks, two the holding tank is all run through to the bottom of connecting block, and the bottom fixedly connected with connecting plate of connecting block, the relative lateral wall of U-shaped mounting bracket rotates and is connected with two-way threaded rod, one side fixedly connected with motor of U-shaped mounting bracket, the top fixedly connected with electric telescopic handle of U-shaped mounting bracket, two be provided with the syringe, two between the splint the bottom of splint respectively with two connecting block fixed connection.

As a further description of the above technical solution:

the sample adding buffer slot is positioned on one side of the high-voltage power supply, the sample adding slot is positioned on one side of the buffer slot far away from the high-voltage power supply, the fixing plate is fixedly connected between the sample adding slot and the buffer slot, and the clamping mechanism is positioned on one side of the sample adding slot far away from the fixing plate.

As a further description of the above technical solution:

and the opposite side walls of the fluorescence detector are fixedly connected with the sample adding groove and the buffer groove respectively.

As a further description of the above technical solution:

and two ends of the capillary tube are respectively communicated with the sample adding groove and the buffer groove.

As a further description of the above technical solution:

two one side that splint are close to each other all is the arc setting, just two the equal fixedly connected with slipmat in one side that splint are close to each other.

As a further description of the above technical solution:

the equal fixedly connected with slider of the relative lateral wall of connecting block, the relative lateral wall of holding tank has all been seted up with the corresponding spout of slider, slider and spout sliding connection.

As a further description of the above technical solution:

the drive end of the motor is fixedly connected with a bidirectional threaded rod, and the bidirectional threaded rod is in threaded connection with the connecting plate.

The utility model discloses following beneficial effect has:

1. the utility model provides a pair of quantitative high accuracy capillary electrophoresis apparatus compares with traditional electrophoresis apparatus, the device simple structure, convenient and practical through motor, two-way threaded rod, splint, the connecting block that the design is simple, can be equipped with the syringe centre gripping that sample solution is equipped with different models to between two splint, has extended the application scope of this device greatly.

2. This structure and design of quantitative high accuracy capillary electrophoresis appearance all have great innovation and improvement, this quantitative high accuracy capillary electrophoresis appearance through starting electric telescopic handle, can promote the syringe to the accurate quantitative application of sample solution that adds in the application of sample groove, and improved the device's detection efficiency greatly, is worth wideling popularize.

Drawings

Fig. 1 is a front view structural diagram of a quantitative high-precision capillary electrophoresis apparatus provided by the present invention;

fig. 2 is a side view structural diagram of a clamping mechanism in a quantitative high-precision capillary electrophoresis apparatus provided by the utility model;

fig. 3 is a partial overlooking structure diagram of a capillary tube in a quantitative high-precision capillary electrophoresis apparatus provided by the present invention;

fig. 4 is a top view structure diagram of the clamping mechanism in the quantitative high-precision capillary electrophoresis apparatus provided by the utility model.

Illustration of the drawings:

1. installing a base; 2. a sample adding slot; 3. a buffer tank; 4. a fixing plate; 5. a clamping mechanism; 6. a capillary tube; 7. a fluorescence detector; 8. a high voltage power supply; 501. a U-shaped mounting bracket; 502. a splint; 503. a non-slip mat; 504. accommodating grooves; 505. connecting blocks; 506. a connecting plate; 507. a bidirectional threaded rod; 508. a motor; 509. an electric telescopic rod; 510. a syringe.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-4, the present invention provides an embodiment: a quantitative high-precision capillary electrophoresis apparatus comprises an installation base 1, wherein the top of the installation base 1 is fixedly connected with a high-voltage power supply 8, a sample adding groove 2, a buffer groove 3, a fixing plate 4 and a clamping mechanism 5, the top of the fixing plate 4 is fixedly provided with a capillary 6, and the top of the fixing plate 4 is provided with a fluorescence detector 7;

the clamping mechanism 5 comprises a U-shaped mounting frame 501 fixedly connected to the top of the mounting base 1, two symmetrical clamping plates 502 are arranged at the top of the U-shaped mounting frame 501, a holding groove 504 is formed at the top of the U-shaped mounting frame 501, two connecting blocks 505 corresponding to the two clamping plates 502 are arranged in the holding groove 504, the bottoms of the two connecting blocks 505 both penetrate through the holding groove 504, a connecting plate 506 is fixedly connected to the bottom of the connecting block 505, a bidirectional threaded rod 507 is rotatably connected to the opposite side wall of the U-shaped mounting frame 501, a motor 508 is fixedly connected to one side of the U-shaped mounting frame 501, an electric telescopic rod 509 is fixedly connected to the top of the U-shaped mounting frame 501, the syringe 510 can be pushed quantitatively to add sample solution into the sample adding groove 2 by starting the electric telescopic rod 509, a syringe 510 is arranged between the two clamping plates 502, the syringe 510 can be used for containing sample solution, and the bottoms of the two clamping plates 502 are respectively and fixedly connected with the two connecting blocks 505, the motor 508 can drive the two-way threaded rod 507 to rotate, so that the two connecting plates 506 can be driven to move towards or away from each other, and the two clamping plates 502 can be driven to move towards or away from each other through the connecting block 505, so that the syringes 510 containing sample solutions of different models can be clamped between the two clamping plates 502.

The sample adding buffer slot 3 is positioned on one side of a high-voltage power supply 8, the sample adding slot 2 is positioned on one side of the buffer slot 3 far away from the high-voltage power supply 8, a fixing plate 4 is fixedly connected between the sample adding slot 2 and the buffer slot 3, a clamping mechanism 5 is positioned on one side of the sample adding slot 2 far away from the fixing plate 4, two poles of the high-voltage power supply 8 are respectively linked with the interiors of the sample adding slot 2 and the buffer slot 3 through leads, opposite side walls of a fluorescence detector 7 are respectively fixedly connected with the sample adding slot 2 and the buffer slot 3, two ends of a capillary 6 are respectively communicated with the sample adding slot 2 and the buffer slot 3, after the high-voltage power supply 8 is switched on, a sample solution in the sample adding slot 2 can enter the capillary 6, the sample solution in the capillary 6 can be detected through the fluorescence detector 7, one side, close to each other, of the two clamping plates 502 are both arranged in an arc shape, one side, close to each other, is fixedly connected with an anti-slip pad 503, and the opposite side wall of a connecting block 505 is fixedly connected with a slide block, the opposite side walls of the accommodating groove 504 are provided with sliding grooves corresponding to the sliding blocks, the sliding blocks are in sliding connection with the sliding grooves, the driving end of the motor 508 is fixedly connected with the bidirectional threaded rod 507, and the bidirectional threaded rod 507 is in threaded connection with the connecting plate 506.

The working principle is as follows: the utility model provides a pair of quantitative high accuracy capillary electrophoresis apparatus has great improvement innovation, when using this kind of quantitative high accuracy capillary electrophoresis apparatus, starter motor 508 can drive two-way threaded rod 507 and rotate, thereby can drive two connecting plates 506 or move dorsad in opposite directions, thereby accessible connecting block 505 drives two splint 502 or move dorsad in opposite directions, thereby can be equipped with sample solution's syringe 510 centre gripping to between two splint 502 with different models, and make the head of syringe 510 be located the top of application of sample groove 2, can quantitatively promote syringe 510 to the application of sample article solution in application of sample groove 2 through starting electric telescopic handle 509, put through high voltage power supply 8 again, sample solution in the application of sample groove 2 can get into in the capillary 6, can dissolve the sample in the capillary 6 and detect through fluorescence detector 7.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides a quantitative high accuracy capillary electrophoresis apparatus, includes installation base (1), its characterized in that: the top of the mounting base (1) is fixedly connected with a high-voltage power supply (8), a sample adding groove (2), a buffer groove (3), a fixing plate (4) and a clamping mechanism (5), the top of the fixing plate (4) is fixedly provided with a capillary tube (6), and the top of the fixing plate (4) is provided with a fluorescence detector (7);

the clamping mechanism (5) comprises a U-shaped mounting frame (501) fixedly connected to the top of the mounting base (1), two symmetrical clamping plates (502) are arranged at the top of the U-shaped mounting frame (501), a holding tank (504) is arranged at the top of the U-shaped mounting frame (501), two connecting blocks (505) corresponding to the two clamping plates (502) are arranged in the holding tank (504), the bottoms of the two connecting blocks (505) penetrate through the holding tank (504), a connecting plate (506) is fixedly connected to the bottom of the connecting block (505), two-way threaded rods (507) are rotatably connected to opposite side walls of the U-shaped mounting frame (501), a motor (508) is fixedly connected to one side of the U-shaped mounting frame (501), an electric telescopic rod (509) is fixedly connected to the top of the U-shaped mounting frame (501), and an injector (510) is arranged between the two clamping plates (502), the bottoms of the two clamping plates (502) are respectively fixedly connected with the two connecting blocks (505).

2. The capillary electrophoresis apparatus of claim 1, wherein: buffer slot (3) are located one side of high voltage power supply (8), application of sample groove (2) are located one side that high voltage power supply (8) were kept away from in buffer slot (3), fixed plate (4) fixed connection is between application of sample groove (2) and buffer slot (3), fixture (5) are located one side that fixed plate (4) were kept away from in application of sample groove (2).

3. The capillary electrophoresis apparatus of claim 1, wherein: and the opposite side walls of the fluorescence detector (7) are respectively fixedly connected with the sample adding groove (2) and the buffer groove (3).

4. The capillary electrophoresis apparatus of claim 1, wherein: and two ends of the capillary tube (6) are respectively communicated with the sample adding groove (2) and the buffer groove (3).

5. The capillary electrophoresis apparatus of claim 1, wherein: two one side that splint (502) are close to each other all is the arc setting, and two the equal fixedly connected with slipmat (503) in one side that splint (502) are close to each other.

6. The capillary electrophoresis apparatus of claim 1, wherein: the opposite side walls of the connecting block (505) are fixedly connected with sliding blocks, the opposite side walls of the accommodating groove (504) are provided with sliding grooves corresponding to the sliding blocks, and the sliding blocks are connected with the sliding grooves in a sliding mode.

7. The capillary electrophoresis apparatus of claim 1, wherein: the driving end of the motor (508) is fixedly connected with a bidirectional threaded rod (507), and the bidirectional threaded rod (507) is in threaded connection with the connecting plate (506).