CN217757507U - Molecular detection kit - Google Patents

Abstract

The application relates to a molecular testing cartridge, comprising: a bottom case having a plurality of receiving cavities; the upper shell is arranged at one end of the bottom shell and is provided with a gun head accommodating hole and a magnetic rod sleeve accommodating hole; the sealing film is arranged between the bottom shell and the upper shell and covers the opening end of the accommodating cavity; the liquid transferring gun head assembly is inserted into the gun head containing hole; the magnetic bar sleeve assembly is inserted into the magnetic bar sleeve accommodating hole; the bottom shell and the upper shell can rotate relatively, so that the gun head containing hole or the magnetic rod sleeve containing hole is correspondingly communicated with any containing cavity; one end of the pipette head component can penetrate through the sealing film and extend into the accommodating cavity correspondingly communicated with the pipette head accommodating hole, and one end of the magnetic rod sleeve component can penetrate through the sealing film and extend into the accommodating cavity correspondingly communicated with the magnetic rod sleeve accommodating hole. Because the molecule detection box integrates the liquid-transferring gun head component and the magnetic rod sleeve component, the molecule detection box can not only transfer the reagent and the sample through the liquid-transferring gun head component, but also transfer the magnetic beads through the magnetic rod sleeve component, and has higher processing speed.

Description

Molecular detection kit

Technical Field

The application relates to the technical field of molecular detection, in particular to a molecular detection box.

Background

Nucleic acids are carriers of genetic information of living bodies and are essential constituent materials for all known life forms. Nucleic acids, DNA and RNA, are mainly present in the nucleus and are present in a state of being bound to proteins. With the rapid development of molecular biology, research and analysis of nucleic acid are continuously popularized and applied in the fields of clinical diagnosis, food safety, environmental detection, agriculture, forestry, animal husbandry and the like.

In the prior art, a centrifugal column method or a magnetic bead method is generally used for extracting nucleic acid, and four steps of cracking, combining, cleaning, eluting and the like are generally required, and the subsequent detection steps of nucleic acid molecular hybridization, polymerase Chain Reaction (PCR), a biochip and the like are added, so that the whole full-automatic instrument from a sample to a result is very difficult to realize. Regarding the transfer of the effective components in each step, the manual transfer mode is mostly adopted in the prior art, the operation is complex, time and labor are wasted, the sample is difficult to be transferred fully and efficiently, the result is unstable easily due to manual operation, and the detection realization difficulty is high.

In order to improve the detection efficiency, enclosed integrated nucleic acid extraction, amplification and detection devices are available on the market, but although these devices can complete a series of operations such as extraction, amplification, detection and the like, these integrated devices usually only rely on a pipette tip to transfer liquid, so that when a magnetic bead method is used for nucleic acid extraction, a pipette tip is required to transfer samples and reagents for multiple times, the experimental efficiency is low, the sample processing speed is significantly influenced, and the further popularization and application of the enclosed integrated nucleic acid extraction, amplification and detection devices are not facilitated.

Disclosure of Invention

In view of the above, it is necessary to provide a molecular test cassette that can achieve the technical effect of increasing the processing speed, in order to solve the problem of the slow processing speed of the conventional integrated nucleic acid extraction, amplification and detection device.

According to an aspect of the present application, there is provided a molecular testing cassette comprising:

the bottom shell is provided with a plurality of accommodating cavities with one ends opened;

the upper shell is arranged at one end of the bottom shell and is close to the opening end of the accommodating cavity, and the upper shell is provided with a gun head accommodating hole and a magnetic rod sleeve accommodating hole;

the sealing film is arranged between the bottom shell and the upper shell and covers the opening end of the accommodating cavity;

the pipette tip component is inserted into the pipette tip containing hole; and

the magnetic rod sleeve assembly is inserted into the magnetic rod sleeve accommodating hole;

the bottom shell and the upper shell can rotate relatively, so that the gun head containing hole or the magnetic rod sleeve containing hole is correspondingly communicated with any one containing cavity; one end of the pipette head assembly can penetrate through the sealing membrane to extend into the accommodating cavity correspondingly communicated with the pipette head accommodating hole, and one end of the magnetic rod sleeve assembly can penetrate through the sealing membrane to extend into the accommodating cavity correspondingly communicated with the magnetic rod sleeve accommodating hole.

In one embodiment, one of the open end of the accommodating cavity and the end of the upper shell connected to the bottom shell is provided with a sliding groove extending along the circumferential direction, and the other of the open end of the accommodating cavity and the end of the upper shell connected to the bottom shell is limited in the sliding groove and can slide along the sliding groove.

In one embodiment, the magnetic rod sleeve assembly comprises a magnetic rod sleeve and a first sealing ring, a connecting part is arranged at one end of the magnetic rod sleeve, the other end of the magnetic rod sleeve is inserted into the magnetic rod sleeve accommodating hole, and the first sealing ring is sleeved outside the magnetic rod sleeve and abuts against the cavity wall of the magnetic rod sleeve accommodating hole.

In one embodiment, the molecular detection cartridge further includes an elastic member, the elastic member is sleeved outside the magnetic rod sleeve, one end of the elastic member is coupled to one end of the magnetic rod sleeve away from the bottom case, the other end of the elastic member abuts against one end wall of the magnetic rod sleeve accommodating hole facing the bottom case, and the elastic member is used for applying an acting force to the magnetic rod sleeve inserted into the accommodating cavity to make the magnetic rod sleeve away from the bottom case.

In one embodiment, the magnetic rod sleeve assembly further comprises a paddle, and the paddle is convexly arranged on the outer side wall of one end, which can extend into the accommodating cavity, of the magnetic rod sleeve.

In one embodiment, the pipette head assembly comprises:

the liquid transfer gun head is inserted into the gun head accommodating hole;

the sealing ring fixing seat is sleeved outside the liquid-transfering gun head; and

the second sealing ring is sleeved outside the sealing ring fixing seat and abuts against the cavity wall of the gun head accommodating hole;

wherein, the external diameter of sealing washer fixing base is greater than bar magnet cover subassembly stretches into hold the external diameter of the one end in chamber, work as the one end of pipetting gun head subassembly stretches into when holding the chamber, the sealing washer fixing base can puncture the seal membrane.

In one embodiment, the upper shell is provided with a plunger accommodating hole, the molecular detection cartridge further comprises a plunger, the plunger is inserted into the plunger accommodating hole, the bottom shell is provided with a liquid injection cavity with one end opened, and one end of the plunger can extend into the liquid injection cavity;

the molecular detection box further comprises a reaction bin, the reaction bin is detachably inserted into the bottom shell and communicated with the liquid injection cavity, and the plunger and the cavity wall of the liquid injection cavity jointly form positive pressure which enables liquid in the liquid injection cavity to flow into the reaction bin.

In one embodiment, the liquid injection cavity is provided with a liquid injection hole and an exhaust hole, the reaction chamber is provided with a detection cavity, and a liquid injection channel and an exhaust channel which are respectively communicated with the detection cavity, one end of the liquid injection channel, which is far away from the detection cavity, is communicated with the liquid injection hole, and one end of the exhaust channel, which is far away from the detection cavity, is communicated with the exhaust hole.

In one embodiment, the plunger comprises a plunger main body, a first sealing part and a second sealing part, the first sealing part and the second sealing part circumferentially surround the outside of one end of the plunger main body and are arranged at intervals in the insertion direction of the plunger, an air exhaust groove is formed between the first sealing part and the second sealing part, and a communication groove for communicating the air exhaust groove and the plunger accommodating hole is formed in the cavity wall of the liquid injection cavity;

in the process that the plunger extends into the liquid injection cavity, the plunger is switched from a first state to a second state;

when the plunger is in the first state, the liquid injection channel is positioned at one side of the plunger at intervals to be communicated with the liquid injection cavity, and the exhaust channel is positioned between the first sealing part and the second sealing part to be communicated with the exhaust groove;

when the plunger is in the second state, the first sealing part seals the liquid injection channel, and the second sealing part seals the exhaust channel.

In one embodiment, the bottom case further defines a sample loading hole, and the molecular detection cartridge further includes at least one sample loading sealing cover, wherein the sample loading sealing cover is detachably inserted into the sample loading hole; and/or

The bottom shell is further provided with a reaction liquid sample adding hole, the molecular detection box further comprises at least one reaction liquid sample adding sealing cover, and the reaction liquid sample adding sealing cover is detachably inserted in the reaction liquid sample adding hole.

Above-mentioned molecular detection box through setting up relative pivoted drain pan and epitheca, can make liquid-transfering gun head subassembly and bar magnet cover subassembly aim at different and hold the chamber to realize reagent and sample and hold the transfer between the chamber in difference. Because this molecular detection box has integrateed pipetting gun head subassembly and bar magnet cover subassembly, consequently both accessible pipetting gun head subassembly transfer reagent and sample, also can transfer the magnetic bead through bar magnet cover subassembly, compare in prior art only adopt the pipetting gun head subassembly to carry out the liquid transfer and can't directly transfer the technical scheme of magnetic bead, the molecular detection box of this application has simplified the transfer flow, has higher processing speed.

Drawings

FIG. 1 is a schematic view of a molecular testing cassette according to an embodiment of the present application;

FIG. 2 is an exploded view of the molecular testing cassette of FIG. 1;

FIG. 3 is a schematic view of the assembly of the magnetic rod sleeve assembly and the pipette head assembly of the molecular testing cassette according to one embodiment of the present application;

FIG. 4 is a schematic view of a magnetic rod sleeve assembly of the molecular testing cassette according to an embodiment of the present application;

FIG. 5 is an assembled view of the bar magnet sleeve assembly of FIG. 4;

FIG. 6 is a schematic view showing the assembly of a magnetic rod sleeve assembly of the molecular testing cassette according to an embodiment of the present application;

FIG. 7 is a schematic view showing the assembly of a plunger and a reaction chamber of the molecular testing cartridge according to the embodiment of the present application;

FIG. 8 is an enlarged view of a portion of FIG. 7 at A;

FIG. 9 is a schematic view of a plunger of a molecular testing cartridge according to an embodiment of the present application in a first state;

FIG. 10 is an enlarged view of a portion of FIG. 9 at B;

FIG. 11 is a schematic view of a plunger of the molecular testing cartridge according to an embodiment of the present application in a second state;

FIG. 12 is an enlarged view of a portion of FIG. 11 at C;

the reference numbers illustrate:

100. a molecular detection cartridge; 10. a bottom case; 12. a bottom shell top wall; 121. an accommodating chamber; 123. a liquid injection cavity; 1232. a liquid injection hole; 1234. an exhaust hole; 1236. a communicating groove; 14. a bottom shell side wall; 141. a limiting groove; 143. a chute seal ring; 20. an upper shell; 21. an upper housing top wall; 211. a gun head accommodating hole; 212. the magnetic rod sleeve accommodating hole; 213. a plunger receiving bore; 214. a sample addition well; 215. a reaction solution sample adding hole; 23. an upper housing sidewall; 30. sealing the film; 40. a magnetic rod sleeve assembly; 41. a magnetic rod sleeve; 412. a connecting portion; 43. a first seal ring; 45. a paddle blade; 50. an elastic member; 60. a pipette tip assembly; 61. a pipette tip; 63. a sealing ring fixing seat; 65. a second seal ring; 70. a plunger; 72. a plunger body; 74. a first seal portion; 76. a second seal portion; 78. an exhaust duct; 80. a reaction bin; 81. a detection chamber; 83. a liquid injection channel; 85. an exhaust passage; 92. a sample adding sealing cover; 94. reaction solution sample feeding sealing cover.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present application more comprehensible, embodiments accompanying the present application are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. This application is capable of embodiments in many different forms than those described herein and that modifications may be made by one skilled in the art without departing from the spirit and scope of the application and it is therefore not intended to be limited to the specific embodiments disclosed below.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless explicitly specified otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and encompass, for example, both fixed and removable connections or integral parts thereof; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to FIG. 1, an embodiment of the present application provides a molecular testing cassette 100, which can be used with a molecular testing apparatus to process and test a target in a sample.

The structure of the molecular cassette 100 according to the present application will be described below, taking a target in a sample as a nucleic acid as an example. It is understood that in other embodiments, the molecular test kit 100 can be used for processing and detecting target substances such as structural proteins, enzymes, antigens, and genes of immunologically active molecules, and is not limited herein. Since the molecular detection instrument is not the main point of the present application, the structure thereof will not be described herein.

As shown in FIGS. 1 to 3, the molecular testing cassette 100 includes a bottom case 10, an upper case 20, a sealing film 30, a pipette head assembly 60, and a magnetic rod set assembly 40. The bottom case 10 has a plurality of receiving chambers 121 opened at one end for receiving a sample and various reagents. The upper shell 20 is mounted at one end of the bottom shell 10 and close to the opening end of the accommodating cavity 121, and the upper shell 20 is provided with a gun head accommodating hole 211 and a magnetic rod sleeve accommodating hole 212. The sealing film 30 is provided between the bottom case 10 and the upper case 20 and covers the open end of the receiving cavity 121. The pipette tip assembly 60 is inserted into the pipette tip containing hole 211, and the magnetic rod sleeve assembly 40 is inserted into the magnetic rod sleeve containing hole 212.

Wherein, the bottom shell 10 and the upper shell 20 can relatively rotate around a rotation axis, so that the gun head containing hole 211 or the magnetic rod sleeve containing hole 212 is communicated with any one of the containing cavities 121. One end of the pipette head assembly 60 can extend into the accommodating cavity 121 correspondingly communicated with the pipette head accommodating hole 211 through the sealing film 30, so that the contents in the accommodating cavity 121 can be transferred under the driving of the molecular detection instrument. One end of the magnetic rod sleeve assembly 40 can extend into the accommodating cavity 121 correspondingly communicated with the magnetic rod sleeve accommodating hole 212 through the sealing film 30, and can oscillate or rotate in the accommodating cavity 121 under the driving of the molecular detection instrument so as to uniformly mix the content in the accommodating cavity 121.

According to the molecular detection kit 100, the bottom shell 10 and the upper shell 20 which can rotate relatively are arranged, so that the pipetting gun head assembly 60 and the magnetic rod sleeve assembly 40 are aligned to different accommodating cavities 121, and thus, the reagents and the samples can be transferred between the different accommodating cavities 121. Because this molecular detection box 100 has integrateed pipetting gun head subassembly 60 and bar magnet cover subassembly 40, both can pass pipetting gun head subassembly 60 transfer sample and reagent, also can pass through bar magnet cover subassembly 40 and transfer the magnetic bead, compare in the prior art and only adopt pipetting gun head subassembly 60 to carry out the transfer of liquid and can't directly transfer the technical scheme of magnetic bead, the molecular detection box 100 of this application has simplified the transfer flow, has higher processing speed.

Referring to fig. 1 to 3, the bottom shell 10 is substantially cylindrical and includes a top wall 12 and a side wall 14 extending from an edge of the top wall 12 in the same direction. Bottom shell top wall 12 is recessed inward to form a plurality of receiving cavities 121, all receiving cavities 121 are arranged at intervals along the circumference of bottom shell top wall 12 at the edge of bottom shell top wall 12, and each receiving cavity 121 has an open end communicating with bottom shell top wall 12. It can be understood that the shape and volume of each accommodating cavity 121 are not limited, and the number of the accommodating cavities 121 can be set as required to meet different experimental requirements. As a preferred embodiment, each receiving cavity 121 extends lengthwise in the axial direction of the bottom case 10, and a cross section of each receiving cavity 121 perpendicular to the axial direction of the bottom case 10 is substantially circular.

The upper case 20 has a substantially cylindrical structure, and the cross-sectional area of the upper case 20 is substantially the same as that of the bottom case 10. The upper shell 20 includes an upper shell top wall 21 and an upper shell side wall 23 formed by extending from the edge of the upper shell top wall 21 in the same direction, the upper shell top wall 21 is recessed inwards to form a gun head accommodating hole 211 and a magnetic rod sleeve accommodating hole 212 with two open ends, and the gun head accommodating hole 211 and the magnetic rod sleeve accommodating hole 212 are respectively located on two opposite sides of the upper shell 20 in the radial direction.

The sealing film 30 is a circular film-shaped structure formed of aluminum, the bottom case 10 and the upper case 20 are separated by the sealing film 30, and the sealing film 30 can seal the open end of the accommodating cavity 121 in the bottom case 10 to prevent the accommodating cavity 121 from being contaminated by the external environment. When it is desired to use chamber 121, pipette head assembly 60 or other element may pierce sealing membrane 30 to expose the open end of chamber 121. It will be appreciated that the material forming the sealing membrane 30 is not limited and may be arranged as desired to meet different sealing requirements.

In some embodiments, one of the open end of the receiving cavity 121 of the bottom case 10 and the end of the top case 20 connected to the bottom case 10 is provided with a sliding groove extending along the circumferential direction, and the other of the two is limited in the sliding groove and can slide along the sliding groove. Specifically, in an embodiment, the sliding groove is formed on the inner side of the upper shell side wall 23 of the upper shell 20, one end of the bottom shell side wall 14 connected to the bottom shell top wall 12 is limited in the sliding groove, one end of the bottom shell side wall 14 limited in the sliding groove is provided with a limit groove 141 extending along the circumferential direction, and at least one sliding groove sealing ring 143 is limited in the limit groove 141 to seal the gap between the bottom shell 10 and the upper shell 20.

As shown in fig. 2 and 3, the rod sleeve assembly 40 includes a rod sleeve 41 and a first seal ring 43. The magnetic rod sleeve 41 has a cylindrical structure with one end open, so that the magnetic rod can extend into the magnetic rod sleeve 41 from the open end of the magnetic rod sleeve 41. The magnetic rod sleeve 41 is inserted into the magnetic rod sleeve accommodating hole 212, and the open end of the magnetic rod sleeve 41 can extend out of the magnetic rod sleeve accommodating hole 212 and is communicated with the open end of the upper shell top wall 21. The first sealing ring 43 is sleeved outside the open end of the magnetic rod sleeve 41 and fixed relative to the magnetic rod sleeve 41, and the outer side wall of the first sealing ring 43 abuts against the cavity wall of the magnetic rod sleeve accommodating hole 212 to seal the gap between the magnetic rod sleeve 41 and the hole wall of the magnetic rod sleeve accommodating hole 212, so that the magnetic rod sleeve assembly 40 is fixed relative to the upper shell 20.

In some embodiments, the molecular testing apparatus has a connector, and the open end of the magnetic rod sleeve 41 is provided with a connecting part 412 matching with the connector, so that the connector can connect the end of the magnetic rod sleeve 41 extending out of the open end of the magnetic rod sleeve accommodating hole 212 through the connecting part 412, and the molecular testing apparatus drives the magnetic rod sleeve 41 to move through the connector. Specifically, one end of the magnetic rod sleeve 41 away from the open end thereof may extend into an accommodating cavity 121 under the driving of the molecular detection instrument, and oscillate in the axial direction of the bottom case 10 under the driving of the molecular detection instrument by overcoming the friction force between the first sealing ring 43 and the cavity wall of the magnetic rod sleeve accommodating hole 212, so as to mix the contents in the accommodating cavity 121 uniformly.

As shown in fig. 4 and fig. 5, in some embodiments, the magnetic rod sleeve assembly 40 further includes a paddle 45, and the paddle 45 is protruded from an outer side wall of an end of the magnetic rod sleeve 41 that can extend into the accommodating cavity 121. Specifically, in one embodiment, the magnetic rod sleeve assembly 40 includes a plurality of blades 45, all of the blades 45 are spaced along the circumference of the magnetic rod sleeve 41, and each blade 45 extends radially outward of the magnetic rod sleeve 41.

In the above embodiment, the connection part 412 of the magnetic rod sleeve 41 is connected to the connection part of the molecular detection instrument, after the end of the magnetic rod sleeve 41 away from the open end of the magnetic rod sleeve extends into the accommodating cavity 121 under the driving of the molecular detection instrument, the molecular detection instrument can drive the magnetic rod sleeve 41 to rotate around its central axis against the friction force between the first sealing ring 43 and the cavity wall of the magnetic rod sleeve accommodating hole 212, and the contents in the accommodating cavity 121 are uniformly mixed under the stirring action of the paddle 45.

In other embodiments, as shown in FIGS. 2 and 6, the molecular testing cassette 100 further comprises a resilient member 50. The elastic element 50 is sleeved outside the magnetic rod sleeve 41, one end of the elastic element 50 is coupled to one end of the magnetic rod sleeve 41 away from the bottom shell 10, the other end of the elastic element 50 abuts against one end wall of the magnetic rod sleeve accommodating hole 212 facing the bottom shell 10, and the elastic element 50 is used for applying a force to the magnetic rod sleeve 41 inserted into the accommodating cavity 121 to make it away from the bottom shell 10. Specifically, in one embodiment, the resilient member 50 is a compression spring that extends helically around the magnet sleeve 41.

In the above embodiment, the molecular detection apparatus includes the holding member that can be extended into the magnetic rod sleeve 41, and the holding member can reciprocate in the axial direction of the upper case 20. Thus, the molecular detection instrument can extend into a containing cavity 121 by the abutting member driving the magnetic rod sleeve 41 to move downwards against the action force of the elastic member 50 and the friction force between the first sealing ring 43 and the cavity wall of the magnetic rod sleeve containing hole 212. When the holding member moves upward, the pressure applied by the holding member to the magnet rod sleeve 41 is reduced or even eliminated, so that the magnet rod sleeve 41 moves upward to the initial position under the driving force applied by the elastic member 50. The above process is repeated, and the magnetic rod sleeve 41 can oscillate in the accommodating cavity 121 to mix the contents in the accommodating cavity 121 uniformly.

Referring again to fig. 2 and 3, the pipette head assembly 60 includes a pipette tip 61, a seal retainer 63, and at least one second seal 65. The pipette tip 61 is a slender hollow revolving body structure, the sealing ring fixing seat 63 is a cylindrical structure to be sleeved outside the pipette tip 61, and the outer diameter of the sealing ring fixing seat 63 is larger than the outer diameter of one end of the magnetic rod sleeve assembly 40 extending into the accommodating cavity 121. All the second sealing rings 65 are sleeved outside the sealing ring fixing seat 63 and abut against the cavity wall of the gun head accommodating hole 211, so that the pipette head assembly 60 is fixed relative to the upper shell 20.

When the pipette tip 61 extends into the accommodating cavity 121, one end of the sealing ring fixing seat 63 can pierce the sealing film 30, so that a crevasse with a diameter larger than that of one end of the magnetic rod sleeve assembly 40 extending into the accommodating cavity 121 is formed on the sealing film 30. Thus, when the magnetic rod sleeve 41 extends into the accommodating cavity 121 through the break and then leaves the accommodating cavity 121 after adsorbing magnetic beads, the magnetic beads adsorbed outside the magnetic rod sleeve 41 cannot scrape the edge of the break and fall off.

As shown in FIGS. 7 and 8, in some embodiments, the molecular testing cassette 100 further includes a reaction chamber 80, and the bottom case 10 has a liquid injection chamber 123. The reaction chamber 80 is detachably inserted into the bottom case 10 and communicates with the injection chamber 123, and nucleic acid extracted from a sample can flow into the reaction chamber 80 from the injection chamber 123 to perform a polymerase chain reaction (PCR reaction).

Specifically, the injection chamber 123 has an open end communicating with the top wall 12 of the base, and the pipette head assembly 60 can inject nucleic acid into the injection chamber 123 through the open end. In order to communicate the reaction chamber 80, the liquid injection chamber 123 is further provided with a liquid injection hole 1232 and an exhaust hole 1234 which communicate the liquid injection chamber 123 with the side wall 14 of the bottom case, the liquid injection hole 1232 and the exhaust hole 1234 are arranged at an interval in the axial direction of the bottom case 10, and the liquid injection hole 1232 is located right below the exhaust hole 1234.

The reaction chamber 80 has a detection chamber 81, and a liquid injection channel 83 and an air discharge channel 85 respectively communicating with the detection chamber 81. When the reaction chamber 80 is inserted into the bottom case 10, one end of the injection channel 83 away from the detection chamber 81 is connected to the injection chamber 123 through the injection hole 1232, and one end of the exhaust channel 85 away from the detection chamber 81 is connected to the injection chamber 123 through the exhaust hole 1234.

Thus, the liquid in the liquid injection chamber 123 can flow into the detection chamber 81 through the liquid injection passage 83, and the gas in the detection chamber 81 can be discharged through the gas discharge passage 85.

Further, in order to inject the liquid in the liquid injection chamber 123 into the reaction chamber 80, the upper case 20 is further opened with plunger accommodating holes 213 opened at both ends. The molecular detection cartridge 100 further comprises a plunger 70, the plunger 70 is inserted into the plunger accommodating hole 213 and can extend into the liquid injection chamber 123, and the plunger 70 and the wall of the liquid injection chamber 123 form a positive pressure together to make the liquid in the liquid injection chamber 123 flow into the reaction chamber 80 under the driving of the molecular detection instrument.

Because the process of pressing the nucleic acid into the reaction bin 80 by the plunger 70 replaces the centrifugal process in the prior art, the nucleic acid can be smoothly injected into the reaction bin 80 without centrifugal treatment, thereby simplifying the nucleic acid extraction steps and improving the nucleic acid extraction efficiency.

Specifically, the plunger 70 includes a plunger body 72, a first seal 74, and a second seal 76. The plunger body 72 has a substantially conical configuration, and the outside diameter of the plunger body 72 gradually increases from one end near the bottom wall of the liquid charging chamber 123 to the other end. The first seal portion 74 and the second seal portion 76 are circumferentially provided around one end of the plunger main body 72 at an interval in the insertion direction of the plunger 70, the vent groove 78 surrounding the plunger main body 72 is formed between the first seal portion 74 and the second seal portion 76 (as shown in fig. 10), and the communication groove 1236 communicating the vent groove 78 with the plunger accommodation hole 213 is opened in the cavity wall of the liquid injection cavity 123.

As shown in fig. 9 to 12, during the process of extending the plunger 70 into the injection chamber 123, the plunger 70 switches from the first state to the second state.

Referring to fig. 9 and 10, when the plunger 70 is in the first state, the filling channel 83 is located at one side of the plunger 70 to communicate with the filling chamber 123, and the vent channel 85 is located between the first sealing portion 74 and the second sealing portion 76 to communicate with the vent groove 78. At this time, the liquid in the liquid injection chamber 123 flows into the detection chamber 81 through the liquid injection passage 83 by the positive pressure generated by the plunger 70, and the gas in the detection chamber 81 flows out through the gas discharge passage 85, the gas discharge groove 78, and the communication groove 1236 in this order.

Referring to fig. 11 and 12, when the plunger 70 is in the second state, the end surface of the plunger 70 abuts against the bottom wall of the injection cavity 123, the length of the plunger 70 extending into the injection cavity 123 reaches the maximum value, at this time, the liquid in the injection cavity 123 is completely injected into the reaction chamber 80, the first sealing portion 74 seals the injection channel 83, and the second sealing portion 76 seals the exhaust channel 85, so as to prevent the liquid in the reaction chamber 80 from leaking through the injection channel 83 and the exhaust channel 85.

Referring to fig. 1 and 2 again, in some embodiments, the bottom housing 10 further defines a sample application hole 214 with two open ends, the sample application hole 214 is located at one side of the accommodating chamber of the pipette tip 61, the molecular testing cassette 100 further includes at least one sample application sealing cap 92, and the sample application sealing cap 92 is detachably inserted into the sample application hole 214 to seal the sample application hole 214. Thus, when it is desired to add a sample to the containing chamber 121, the sample addition sealing cap 92 can be opened and the sealing film 30 can be punctured to add the sample to the containing chamber 121.

In some embodiments, the bottom housing 10 further defines a reaction solution loading hole 215 with two open ends, the reaction solution loading hole 215 is located at one side of the magnetic rod sleeve 41 for accommodating the cartridge, and the molecular detection cartridge 100 further includes at least one reaction solution loading sealing cover 94, and the reaction solution loading sealing cover 94 is detachably inserted into the reaction solution loading hole 215 to seal the reaction solution loading hole 215. In this manner, when it is necessary to add the reaction liquid to the housing chamber 121, the reaction liquid addition hole 215 may be opened and the sealing film 30 may be pierced to add the reaction liquid to the housing chamber 121.

The detection process of the above-described molecular detection cartridge 100 is as follows:

first, the sample loading sealing cap 92 and the reaction solution loading sealing cap 94 are opened, the sealing film 30 is punctured at the corresponding region to load the sample and the reaction solution into the accommodating chamber 121 of the bottom housing 10, and then the molecular detection cartridge 100 is coupled to the molecular detection instrument, which fixes and limits the upper housing 20 and drives the bottom housing 10 to rotate relative to the upper housing 20.

Then, the molecular detection apparatus sucks the solution such as the proteinase K reagent and the magnetic beads, which is pre-packaged in the containing cavity 121, through the pipette head assembly 60 and injects the solution into the containing cavity 121 containing the sample. The molecular detection instrument drives the pipette head assembly 60 to ascend to be separated from the top wall 12 of the bottom shell, drives the bottom shell 10 to rotate until the magnetic rod sleeve assembly 40 is positioned right above the containing cavity 121 containing the sample, and then drives the magnetic rod sleeve assembly 40 to vibrate or rotate for mixing. After the uniform mixing is completed, the magnetic rod in the molecular detection instrument extends into the magnetic rod sleeve 41 for magnetic attraction, the magnetic beads in the accommodating cavity 121 are adsorbed to the outer surface of the magnetic rod sleeve 41, and then the magnetic rod sleeve 41 is driven to ascend to be separated from the accommodating cavity 121.

Then, the molecular detection instrument drives the bottom case 10 to rotate until the containing cavity 121 containing the washing liquid is located under the magnetic rod sleeve 41, the magnetic rod sleeve 41 and the magnetic rod in the molecular detection instrument synchronously move downwards to be inserted into the containing cavity 121 containing the washing liquid, then the magnetic rod moves upwards to leave the containing cavity 121, magnetic beads adsorbed on the magnetic rod sleeve 41 fall into the washing liquid, the molecular detection instrument drives the magnetic rod sleeve 41 to vibrate or rotate in the containing cavity 121 for uniform mixing, after the uniform mixing is completed, the magnetic rod enters the magnetic rod sleeve 41 again to adsorb the magnetic beads in the washing liquid, the magnetic beads in the washing liquid are adsorbed to the outer surface of the magnetic rod sleeve 41, and then the magnetic rod sleeve 41 is driven to ascend to be separated from the containing cavity 121.

After the washing process is repeated for a plurality of times, the same separation can be used for elution, the magnetic beads after elution can be transferred to the used containing cavity 121 through the magnetic rod sleeve 41 and the magnetic rod, the liquid-transferring gun head assembly 60 can suck the eluted nucleic acid solution to be uniformly mixed in the containing cavity 121 containing the reaction liquid, and then a certain amount of mixed solution is sucked and injected into the liquid injection cavity 123.

Finally, the molecular detection apparatus drives the plunger 70 to move downwards to inject the mixed solution in the injection chamber 123 into the reaction chamber 80, and the nucleic acid finally performs the polymerase chain reaction in the reaction chamber 80.

The molecular testing cassette 100 can make the pipette head assembly 60 and the magnetic rod sleeve assembly 40 extend into different accommodating cavities 121 to transfer samples and reagents only by the relative rotation of the bottom shell 10 and the top shell 20. Moreover, the liquid and magnetic beads are transferred by using the pipette head assembly 60 and the magnetic rod sleeve assembly 40, so that the number of experimental steps can be reduced remarkably and the processing speed can be increased compared with the method of transferring liquid only in the prior art. Moreover, the nucleic acid sample is pressurized and injected into the reaction chamber 80 by the plunger 70, so that the centrifugation process in the prior art is omitted, the nucleic acid extraction process is simplified, the experiment cost is saved, and the experiment efficiency is improved. In addition, the whole detection process by utilizing the nucleic acid detection box is carried out in the closed nucleic acid detection box, so that the detection environment and the experience of detection personnel are not excessively required, and the nucleic acid detection box has wide application prospect.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A molecular testing cassette, comprising:

the bottom shell is provided with a plurality of accommodating cavities with one ends opened;

the upper shell is arranged at one end of the bottom shell and close to the opening end of the accommodating cavity, and is provided with a gun head accommodating hole and a magnetic rod sleeve accommodating hole;

the sealing film is arranged between the bottom shell and the upper shell and covers the opening end of the accommodating cavity;

the liquid transferring gun head assembly is inserted into the gun head containing hole; and

the magnetic rod sleeve assembly is inserted into the magnetic rod sleeve accommodating hole;

the bottom shell and the upper shell can rotate relatively, so that the gun head containing hole or the magnetic rod sleeve containing hole is correspondingly communicated with any one containing cavity; one end of the pipette head assembly can penetrate through the sealing film and stretch into the accommodating cavity correspondingly communicated with the pipette head accommodating hole, and one end of the magnetic rod sleeve assembly can penetrate through the sealing film and stretch into the accommodating cavity correspondingly communicated with the magnetic rod sleeve accommodating hole.

2. The molecular testing cassette of claim 1, wherein one of the open end of the receiving chamber and the end of the upper housing connected to the bottom housing is provided with a sliding groove extending along the circumferential direction, and the other of the open end of the receiving chamber and the end of the upper housing connected to the bottom housing is retained in the sliding groove and can slide along the sliding groove.

3. The molecular testing cassette of claim 1, wherein the magnetic rod sleeve assembly comprises a magnetic rod sleeve and a first sealing ring, one end of the magnetic rod sleeve is provided with a connecting portion, the other end of the magnetic rod sleeve is inserted into the magnetic rod sleeve accommodating hole, and the first sealing ring is sleeved outside the magnetic rod sleeve and abuts against the cavity wall of the magnetic rod sleeve accommodating hole.

4. The molecular testing cassette of claim 3, further comprising an elastic member, wherein the elastic member is sleeved outside the magnetic rod sleeve, one end of the elastic member is coupled to an end of the magnetic rod sleeve away from the bottom case, the other end of the elastic member abuts against an end wall of the magnetic rod sleeve accommodating hole facing the bottom case, and the elastic member is configured to apply an acting force to the magnetic rod sleeve inserted into the accommodating cavity to make the magnetic rod sleeve away from the bottom case.

5. The molecular testing cassette of claim 3, wherein the magnetic rod sleeve assembly further comprises a paddle protruding from an outer sidewall of an end of the magnetic rod sleeve that extends into the receiving cavity.

6. The molecular testing cassette of claim 1, wherein the pipetting gun head assembly comprises:

the liquid-transfering gun head is inserted in the gun head accommodating hole;

the sealing ring fixing seat is sleeved outside the liquid-transfering gun head; and

the second sealing ring is sleeved outside the sealing ring fixing seat and abuts against the cavity wall of the gun head accommodating hole;

wherein, the external diameter of sealing washer fixing base is greater than bar magnet cover subassembly stretches into the external diameter of the one end that holds the chamber works as the one end of pipetting gun head subassembly stretches into when holding the chamber, the sealing washer fixing base can puncture the seal membrane.

7. The molecular testing cassette of claim 1, wherein the upper housing defines a plunger receiving hole, the molecular testing cassette further comprising a plunger inserted into the plunger receiving hole, the bottom housing having a liquid injection cavity with an open end, the plunger having an end capable of extending into the liquid injection cavity;

the molecular detection box further comprises a reaction bin, the reaction bin is detachably inserted in the bottom shell and communicated with the liquid injection cavity, and the plunger and the cavity wall of the liquid injection cavity jointly form positive pressure which enables liquid in the liquid injection cavity to flow into the reaction bin.

8. The molecular testing cassette of claim 7, wherein the injection chamber has an injection hole and an exhaust hole, the reaction chamber has a testing chamber and an injection channel and an exhaust channel respectively communicating with the testing chamber, the end of the injection channel far away from the testing chamber communicates with the injection hole, and the end of the exhaust channel far away from the testing chamber communicates with the exhaust hole.

9. The molecular testing cassette of claim 8, wherein the plunger comprises a plunger body, a first sealing portion and a second sealing portion, the first sealing portion and the second sealing portion circumferentially surround the outside of one end of the plunger body and are arranged at intervals in the insertion direction of the plunger, an exhaust groove is formed between the first sealing portion and the second sealing portion, and a communication groove communicating the exhaust groove and the plunger accommodating hole is formed in the cavity wall of the liquid injection cavity;

in the process that the plunger extends into the liquid injection cavity, the plunger is switched from a first state to a second state;

when the plunger is in the first state, the liquid injection channel is positioned at one side of the plunger at intervals to be communicated with the liquid injection cavity, and the exhaust channel is positioned between the first sealing part and the second sealing part to be communicated with the exhaust groove;

when the plunger is in the second state, the first sealing part seals the liquid injection channel, and the second sealing part seals the exhaust channel.

10. The molecular testing cassette of claim 1, wherein the bottom housing further defines a sample loading hole, and the molecular testing cassette further comprises at least one sample loading sealing cover, and the sample loading sealing cover is detachably inserted into the sample loading hole; and/or

The bottom shell is further provided with a reaction liquid sample adding hole, the molecular detection box further comprises at least one reaction liquid sample adding sealing cover, and the reaction liquid sample adding sealing cover is detachably inserted in the reaction liquid sample adding hole.

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