CN217757483U - Experimental device for nucleic acid amplification detection - Google Patents
Experimental device for nucleic acid amplification detection Download PDFInfo
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- CN217757483U CN217757483U CN202221724024.8U CN202221724024U CN217757483U CN 217757483 U CN217757483 U CN 217757483U CN 202221724024 U CN202221724024 U CN 202221724024U CN 217757483 U CN217757483 U CN 217757483U
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- acid amplification
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Abstract
The utility model discloses an experimental device for nucleic acid amplification detection, which comprises a first experimental mechanism and a second experimental mechanism; the first experimental mechanism comprises a first box body component containing a plurality of samples; the second experimental mechanism comprises a second box component, a diffuser, a first exhaust component and a second exhaust component, wherein the first exhaust component is configured to promote aerosol formed in the sample to flow to the outside through the diffuser and the second exhaust component in sequence. The utility model discloses simple structure, be convenient for operation and transport are applicable to the work occasion under the multiple different environment.
Description
Technical Field
The utility model relates to a gene amplification field specifically relates to an experimental apparatus for be used for nucleic acid amplification to detect.
Background
With the continuous development of science and technology, the demand on laboratories is increasing day by day. Therefore, in recent years, many important laboratories have come out in various subject fields.
In routine testing in the laboratory, it is often necessary to handle a large number of test samples from different sources. However, since some samples are difficult to degrade, cross contamination between samples is easily caused, thereby affecting the accuracy of the test of the experimental samples. For example, a trace amount of nucleic acid can cause a false positive detection result in the test sample, thereby causing a false determination of the detection result. Therefore, if left untreated, this can lead to contamination of the entire laboratory and thus to greater losses.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a from experimental apparatus who is used for nucleic acid amplification to detect, it can be under the effect of fan to the inside ventilation that carries out of experimental apparatus who is used for nucleic acid amplification to detect to guarantee that the sample is in pollution-free state at the in-process that detects, and then improved the accuracy of testing result, further improved the efficiency that detects. Furthermore.
According to the utility model, an experimental device for nucleic acid amplification detection is provided, which comprises a first experimental mechanism and a second experimental mechanism; the first experiment mechanism comprises a first box body assembly used for containing a plurality of samples and capable of receiving external air; the second experiment mechanism comprises a second box body component arranged above the first experiment mechanism, a diffuser arranged on the bottom surface of the second box body component and used for guiding fluid from the first box body component, a first exhaust component arranged in the second box body component, and a second exhaust component arranged on the top surface of the second box body component and used for guiding fluid, wherein the first exhaust component is configured to enable aerosol formed in the sample to be promoted to flow to the outside through the diffuser and the second exhaust component in sequence.
In one embodiment, the second vent assembly includes a first through-hole extending in a vertical direction through a top surface of the second tank assembly, and a check valve disposed below the first through-hole, wherein the check valve is partially embedded in the second tank assembly.
In one embodiment, the second exhaust assembly further comprises a pipe member disposed above the first through hole, wherein one end of the pipe member is sealingly connected with the first through hole, and a second end thereof communicates with the outside.
In one embodiment, the first exhaust assembly includes an extension extending in a lateral direction from the first side of the second case assembly, and a fan fixedly coupled to the extension.
In one embodiment, the first box assembly comprises three fixed side plates and one movable side plate which are arranged along the circumferential direction, wherein the movable side plate is configured into a square structure and can move into the second box assembly or reset onto the first box assembly along the vertical direction.
In one embodiment, the first box assembly further comprises a square baffle plate respectively arranged at the bottom of the fixed side plate and the movable side plate, a plurality of second through holes arranged on the square baffle plate and used for receiving external air, and a filter layer arranged in the square baffle plate along the circumferential direction.
In one embodiment, a lamp for an operation mode and a uv lamp for a maintenance mode are provided on a top surface of the first tank assembly.
In one embodiment, an atomizer is provided on the second side of the first tank assembly for degrading aerosol in the maintenance mode.
In one embodiment, a plurality of sealing strips are arranged at the bottom of the square baffle, and the sealing strips can be in sealing fit with the experiment table.
In one embodiment, handles configured in an arcuate configuration are provided on both the second box assembly and the movable side panel.
Drawings
The invention will be described in detail below with reference to the attached drawings, in which:
FIG. 1 schematically shows the structure of an experimental apparatus for nucleic acid amplification detection according to the present invention;
FIG. 2 is a schematic diagram of the internal structure of the experimental apparatus for nucleic acid amplification detection according to the present invention;
FIG. 3 is a schematic partial structural diagram of the experimental apparatus for nucleic acid amplification detection according to the present invention, which shows the position relationship between the square baffle and the sealing strip.
In the drawings, like parts are given like reference numerals. The figures are not drawn to scale.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
FIG. 1 schematically shows the structure of an experimental apparatus for nucleic acid amplification detection according to the present invention. As shown in fig. 1, the experiment apparatus 100 for nucleic acid amplification detection according to the present invention mainly includes a first experiment mechanism 10 and a second experiment mechanism 11. Wherein the first experiment mechanism 10 is disposed below the second experiment mechanism 11, and the inside of the first experiment mechanism 10 can simultaneously accommodate a plurality of samples (not shown). Further, the first experimental facility 10 communicates with the outside, and is capable of receiving outside air. The second laboratory mechanism 11 is configured to be able to transport the gas from within the first laboratory mechanism 10 to the outside together with the aerosol formed within the sample.
According to the present invention, as shown in fig. 1, the first experiment mechanism 10 includes a first case assembly 20. The first case assembly 20 is constructed as a square case without a base. In this way, the experimental device 100 for nucleic acid amplification detection can be directly placed on the experiment table for detection, thereby effectively improving the working efficiency.
Fig. 2 is a schematic diagram of the internal structure of the experimental apparatus 100 for nucleic acid amplification detection according to the present invention. According to the present invention, as shown in fig. 2, the second experiment mechanism 11 includes a second box assembly 30 and a diffuser. Wherein the second case assembly 30 is disposed above the first case assembly 20, and the second case assembly 30 is fixedly connected to the first case assembly 20. A diffuser is provided at the bottom of the second tank assembly 30 for guiding the fluid (referred to as gas and aerosol) in the first tank assembly 20 into the second tank assembly 30. The operating principle of the diffuser is well known to the person skilled in the art and will therefore not be described in further detail.
According to the present invention, as shown in fig. 2, the second experiment mechanism 11 further includes a second exhaust assembly 50. The second exhaust assembly 50 is provided on the top surface of the second tank assembly 30 and can communicate with the outside. According to an embodiment of the present invention, the second exhaust assembly 50 can guide the fluid inside the second tank assembly 30 to the outside. The contents of which are described below.
According to the present invention, as shown in fig. 2, the second experiment mechanism 11 further includes a first exhaust assembly 40. The first exhaust assembly 40 is disposed within the interior of the second tank assembly 30 and generally intermediate the diffuser and the second exhaust assembly 50. According to an embodiment of the present invention, the first exhaust assembly 40 is configured to cause the fluid to flow to the outside through the first tank assembly 20, the diffuser, and the second exhaust assembly 50 in sequence.
According to the present invention, as shown in fig. 2, the second exhaust assembly 50 includes a first through hole 51 and a check valve 52. The first through hole 51 extends vertically through the top surface of the second tank assembly 30 and can guide gas inside the second tank assembly 30 to the outside through a passage member (described later). A check valve 52 is disposed below the first through hole 51, and specifically, the check valve 52 is partially embedded in the second tank assembly 30. In this way, the non-return valve 52 is enabled to control the gas flow direction more easily and more comprehensively. The working principle of the non-return valve 52 is well known to the person skilled in the art and is therefore not described in further detail.
According to the present disclosure, the second exhaust assembly 50 further includes a pipe (not shown). A first end of the pipe member is sealingly connected to the first through hole 51 and a second end of the pipe member communicates with the outside. Thus, the fluid flowing out of the first through hole 51 can be directly discharged to the outside through the pipe member, thereby effectively securing the detection environment in the experimental apparatus for nucleic acid amplification detection.
According to the present invention, as shown in fig. 2, the first exhaust assembly 40 includes an extension 41 and a fan 42. Wherein the extension 41 is disposed to extend in a lateral direction from the first side 32 of the second casing assembly 30. The fan 42 is fixedly connected to the free end of the extension 41 and is located approximately in the middle of the second case assembly 30. In this way, the fan 42 can deliver the fluid guided by the diffuser to the outside through the first through holes 51 and the duct member more lumpy and sufficiently.
According to the present invention, as shown in fig. 1, the first case assembly 20 includes a fixed side plate 22 and a movable side plate 23. Wherein, the fixed side plate 22 is provided with three and the movable side plate 23 is provided with one, and the fixed side plate 22 and the movable side plate 23 are arranged along the same circumference. In this way, the experimental apparatus 100 for nucleic acid amplification detection has a more stable and safer structure.
According to an embodiment of the present invention, the movable side plate 23 is constructed in a square structure and can move in a vertical direction, thereby realizing the opening and closing of the first case assembly 20. Specifically, the movable side panel 23 can be moved upward into the second case assembly 30, thereby opening the first case assembly 20 and allowing an operation mode (described below) to be entered. The movable side plate 23 can be moved downward to be restored to the first casing assembly 20, thereby closing the first casing assembly 20 and enabling a maintenance mode (described later) to be entered.
According to the present invention, as shown in fig. 1, the first case assembly 20 further includes a square baffle 24. The square baffle 24 is disposed below the fixed side plate 22 and the movable side plate 23, and a plurality of second through holes 241 are further provided in the square baffle 24. Thus, the external air can be introduced into the first casing assembly 20 through the second through hole 241 by the blower fan 42. In an embodiment of the present invention, as shown in fig. 3, the second through hole 241 is preferably a rectangular parallelepiped through hole. Thus, the external air can be more quickly and easily introduced into the first case assembly 20 through the second through-hole 241.
In accordance with an embodiment of the present invention, a filter layer (not shown) is also provided within the square baffle 24. The filter layer is circumferentially extended around the square-shaped baffle 24 for filtering the gas entering from the second through-holes 241. It is easily understood that the filter layer generally functions to filter the gas entering the experiment device 100 for nucleic acid amplification detection through the second through hole 241 with the movable side plate 23 closed.
According to the present invention, as shown in FIG. 2, the experimental device 100 for nucleic acid amplification detection further includes an illumination lamp 25 and an ultraviolet lamp 26. Among them, the illumination lamp 25 is installed on the top surface of the first case assembly 20, and is mainly used for an operation mode. The ultraviolet lamp 26 is mounted on the top surface of the first tank assembly 20 and is primarily used in the maintenance mode.
According to the present invention, as shown in fig. 2, the experiment device 100 for detecting nucleic acid amplification further includes an atomizer 211. The atomizer 211 is provided in the first casing assembly 20, and specifically, the atomizer 211 is mounted on the second side 21 of the first casing assembly 20. According to an embodiment of the present invention, the atomizer 211 can spray the atomizing agent dissolving the aerosol in the maintenance mode as described above, thereby ensuring that the inside of the experimental apparatus 100 for nucleic acid amplification detection can be formed into a contamination-free environment. The operating principle of the atomizer 211 is well known to those skilled in the art.
According to one embodiment of the present invention, as shown in fig. 2, a plurality of sealing strips 27 are provided at the bottom of the square baffle 24. When the test device 100 for nucleic acid amplification detection is placed on a test table, the seal strip 27 can be sealingly attached to the top surface of the test table. In this way, the experimental apparatus 100 for nucleic acid amplification detection can have better stability during operation.
In one embodiment of the present invention, as shown in fig. 2, handles 60 configured in an arc structure are provided at both sides of the second casing assembly 30. In this way, the test device 100 for nucleic acid amplification detection can be more easily handled. A handle 60 configured in an arc structure is provided on the movable side plate 23. In this way, the movable side plate 23 can be more easily moved in the vertical direction.
In one embodiment of the present invention, a touch screen 70 is further provided on one side of the second casing assembly 30. The operation mode or the maintenance mode as described above can be selected through the touch screen 70.
The operation process of the utility model comprises the following steps.
At first, select clean room, will the utility model discloses put on clean and stable, the laboratory bench that is close to the window, sealing strip 27 and the sealed laminating in surface of laboratory bench this moment.
Then, one end of the pipe member is hermetically connected to the first through hole 51, and the other end is opened to the outside;
then, the movable side plate 23 is pushed up, and then the sample (nucleic acid sample) is put into the utility model;
then, the power is switched on, the working mode is selected on the touch screen 70, 5-10 minutes of waiting are carried out, and the experimental operation is carried out after the airflow is in a stable state:
finally, after the operation is finished, the maintenance mode is selected on the touch screen 70.
The working mode is as follows: the illumination lamp 25 and the blower fan 42 are turned on, the ultraviolet lamp 26 is turned off, and the air flows to the outside through the second through hole 241, the first case assembly 20, the diffuser 31, the blower fan 42, the check valve 52, the first through hole 51, and the passage member in sequence by the blower fan 42. The aerosol formed in the experiment process flows to the outside through the diffuser 31, the fan 42, the check valve 52, the first through hole 51 and the passage member in sequence under the driving of the gas.
Maintenance mode (including daily and weekly maintenance).
Daily maintenance: the ultraviolet lamp 26 is turned on, the illuminating lamp 25 and the fan 42 are turned off, and the ultraviolet lamp 26 is automatically turned off after being sterilized by ozone for 30 min.
Maintenance is carried out every week: the atomizer 211 sprays maintenance liquid for degrading aerosol, and automatically closes after spraying for 5 min.
In one embodiment of the present invention, a manual mode may also be selected within the touch screen 70. The various modules within the assay device for nucleic acid amplification detection can be selectively adjusted in a manual mode.
It is readily understood that nucleic acid amplification detection techniques include: PCR technology, nucleic acid isothermal amplification technology, etc.
In a first embodiment of the present invention (taking PCR as an example), the following contents are included:
the experimental scheme is as follows: the experiment is to detect the pathogen of the penaeus vannamei boone, namely the enterocytozoon hepatica, and detect the amplification reaction liquid added with the positive plasmid of the enterocytozoon hepatica by using a membrane chip method and utilizing a common PCR amplification instrument for amplification and an amplified product.
Experimental equipment: an experimental device 100 for nucleic acid amplification detection, a PCR amplification instrument, a micro-porous plate constant temperature oscillator, a vortex instrument, a small centrifuge, a pipettor and a PCR tube.
Experimental materials: samples (Penaeus vannamei-liver enterocytozoon positive sample, negative sample and negative control (such as water), amplification reaction solution, reaction solution 1, reaction solution 2, reaction solution 3, cleaning solution and chip.
A preparation stage:
1. the experiment device 100 for nucleic acid amplification detection is set on a horizontal bench, and the piping member is communicated with the outside.
2. A microplate thermostated oscillator, vortexer, mini centrifuge, and pipettor are placed within the assay device 100 for nucleic acid amplification detection.
3. The amplification reaction solution is evenly distributed into 6 PCR tubes, and 3 mu L of a penaeus vannamei boone-enterocytozoon positive sample, a negative sample and a negative control substance are respectively added into the PCR tubes.
4. And (3) uniformly mixing the PCR tubes added with the samples, centrifuging and placing the mixture into a PCR amplification instrument for amplification.
5. After the amplification is completed, the product is analyzed in the experimental apparatus 100 for detecting nucleic acid amplification.
Experimental stage (product analysis):
1. the experimental apparatus 100 for nucleic acid amplification detection is powered on in advance, and the operating state is selected on the touch screen 70, and at this time, the fan 42 and the illumination lamp 25 start to operate.
2 respectively electrifying and starting the microporous plate constant-temperature oscillator, the vortex instrument and the small centrifuge, and adjusting the temperature of the microporous plate constant-temperature oscillator to 45 ℃.
3. Taking out crystal lattices with the corresponding number of samples (placing chips in the crystal lattices in advance), and adding 600 mu L of reaction liquid 1 and a PCR amplification product of a sample to be detected into a reaction tank for each product; transferring the crystal lattice into a microplate constant temperature oscillator at 1500rpm for 10min; and after the reaction is finished, taking out the crystal lattice, opening the cover, and discarding the liquid in each reaction tank.
4. According to the detection quantity, firstly premixing a reaction solution 1 and a reaction solution 2; 600. Mu.L of the reaction solution 1 and the reaction solution 2 premix was added to each reaction vessel at 1500rpm for 3min; and after the reaction is finished, taking out the crystal lattice, opening the cover, and discarding the liquid in each reaction tank.
5. Adding 2mL of cleaning solution into each reaction tank, covering, placing in a microplate constant-temperature oscillator at 1500rpm for 2min; and after the reaction is finished, taking out the crystal lattice, opening the cover, and discarding the liquid in each reaction tank.
6. Adding 500. Mu.L of the reaction solution 3 into each reaction tank, covering the reaction tank, placing the reaction tank on a microplate constant temperature oscillator at 1500rpm for 3min; and opening the cover, adding 2-3 mL of tap water into each reaction tank again, slightly shaking the crystal lattice for 5s, and then terminating the reaction. The reaction results were directly observed for each chip in water.
8. After the experiment is finished, daily maintenance is selected on the touch screen 70, and the machine is automatically shut down after maintenance.
In the above experiment stage, the experiment apparatus 100 for nucleic acid amplification detection can effectively discharge all the pollutants such as aerosol generated in the experiment process to the outside, thereby avoiding the pollution to the experiment sample, improving the accuracy of the detection result, and simultaneously improving the detection efficiency.
The utility model provides an experimental apparatus for be used for nucleic acid amplification to detect, it can be under fan 42's effect to being used for the inside ventilation that carries out of experimental apparatus 100 that nucleic acid amplification detected to guarantee that the sample is in pollution-free state at the in-process that detects, and then improved the accuracy of testing result, further improved the efficiency that detects. Furthermore, the utility model discloses the volume is less, be convenient for operation and transport, is applicable to the work occasion under the multiple different environment.
The above is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto. Those skilled in the art can easily make changes or variations within the scope of the present disclosure, and such changes or variations are intended to be covered by the scope of the present disclosure. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. An assay device for nucleic acid amplification detection, comprising:
a first experimental mechanism (10) and a second experimental mechanism (11);
the first experimental mechanism (10) comprises a first box body component (20) used for containing a plurality of samples and capable of receiving external air;
the second experiment mechanism (11) comprises a second box body component (30) arranged above the first experiment mechanism (10), a diffuser (31) arranged on the bottom surface of the second box body component (30) and used for guiding the fluid from the first box body component (20), a first exhaust component (40) arranged in the second box body component (30), and a second exhaust component (50) arranged on the top surface of the second box body component (30) and used for guiding the fluid,
wherein the first exhaust assembly (40) is configured to cause aerosol formed within the sample to flow sequentially through the diffuser (31) and the second exhaust assembly (50) to the outside.
2. The experimental device for nucleic acid amplification detection according to claim 1, wherein the second vent assembly (50) comprises a first through hole (51) extending in a vertical direction through a top surface of the second case assembly (30), and a check valve (52) disposed below the first through hole (51), wherein the check valve (52) is partially embedded in the second case assembly (30).
3. The experimental device for nucleic acid amplification detection according to claim 2, wherein the second vent assembly (50) further comprises a pipe member disposed above the first through hole (51), wherein one end of the pipe member is hermetically connected to the first through hole (51), and the second end thereof is in communication with the outside.
4. The experimental apparatus for nucleic acid amplification detection according to claim 3, wherein the first exhaust assembly (40) includes an extension (41) extending in a lateral direction from the first side surface (32) of the second casing assembly (30), and a blower (42) fixedly connected to the extension (41).
5. The experimental apparatus for nucleic acid amplification detection according to claim 4, wherein the first case assembly (20) comprises three fixed side plates (22) and one movable side plate (23) which are arranged along the circumferential direction, wherein the movable side plate (23) is configured into a square structure and can move into the second case assembly (30) or reset onto the first case assembly (20) along the vertical direction.
6. The experimental apparatus for nucleic acid amplification detection according to claim 5, wherein the first case assembly (20) further includes a square baffle plate (24) disposed at the bottom of the fixed side plate (22) and the movable side plate (23), respectively, a plurality of second through holes (241) disposed on the square baffle plate (24) for receiving external air, and a filter layer disposed circumferentially inside the square baffle plate (24).
7. The experimental device for nucleic acid amplification detection according to any one of claims 1 to 6, wherein an illumination lamp (25) for an operation mode and an ultraviolet lamp (26) for a maintenance mode are provided on a top surface of the first tank assembly (20).
8. The experimental apparatus for nucleic acid amplification detection according to claim 7, wherein a nebulizer (211) is provided on the second side surface (21) of the first case assembly (20) for degrading aerosol in the maintenance mode.
9. The experimental device for nucleic acid amplification detection according to claim 6, wherein a plurality of sealing strips (27) are arranged at the bottom of the square baffle plate (24), and the sealing strips (27) can be in sealing fit with the experimental bench.
10. The experimental apparatus for nucleic acid amplification detection according to claim 5, wherein handles (60) configured in an arc-shaped configuration are provided on both the second case assembly (30) and the movable side plate (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221724024.8U CN217757483U (en) | 2022-07-06 | 2022-07-06 | Experimental device for nucleic acid amplification detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221724024.8U CN217757483U (en) | 2022-07-06 | 2022-07-06 | Experimental device for nucleic acid amplification detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217757483U true CN217757483U (en) | 2022-11-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221724024.8U Active CN217757483U (en) | 2022-07-06 | 2022-07-06 | Experimental device for nucleic acid amplification detection |
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|---|---|
| CN (1) | CN217757483U (en) |
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2022
- 2022-07-06 CN CN202221724024.8U patent/CN217757483U/en active Active
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