CN217739000U - Fluorescence detection system for biochip - Google Patents
Fluorescence detection system for biochip Download PDFInfo
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- CN217739000U CN217739000U CN202220827637.8U CN202220827637U CN217739000U CN 217739000 U CN217739000 U CN 217739000U CN 202220827637 U CN202220827637 U CN 202220827637U CN 217739000 U CN217739000 U CN 217739000U
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Abstract
The utility model discloses a fluorescence detection system for a biochip, which comprises a bracket and an imaging acquisition component vertically arranged on the bracket, wherein a first optical filter is arranged below the imaging acquisition component; a light source assembly is arranged below the first light filter and comprises a light source rotating disc, the light source rotating disc is coaxial with the imaging acquisition assembly, and a white light source is arranged in the center of the light source rotating disc; the light source turntable is provided with a plurality of pairs of monochromatic light sources, each pair of monochromatic light sources is symmetrical about the center of the light source turntable, the light emitting ends of the monochromatic light sources point to the position right above the white light source, and the light emitting ends of the monochromatic light sources are provided with second optical filters. The utility model has the advantages that: the detection indexes such as large detection view field, high resolution and the like can be simultaneously met.
Description
Technical Field
The utility model relates to a fluorescence detection field, concretely relates to fluorescence detecting system for biochip.
Background
In recent years, single molecule detection technologies represented by digital PCR and digital ELISA have been developed rapidly, and the change of target detection dimension has made higher requirements on the performance of fluorescence detection systems (such as higher optical path uniformity, larger detection field (see, for example, wangzhong et al, design of large-field fluorescence microscopy system applied to dPCR, optical design, 2021, vol. 41, no. 1: 6), higher resolution, etc.). In the prior art, for example, chinese patent application publication No. CN112345503A discloses a multiple fluorescence detection apparatus, which is aligned with a sample, and includes: a lens positioned directly above the sample and aligned with the sample; the annular lighting device is sleeved on the periphery of the lens and is annularly provided with a plurality of luminous points; a plurality of laser light sources that emit laser light, respectively; optical fibers connecting the light emitting points of the annular lighting device and the laser light sources; a camera positioned right above the lens and aligned with the lens for collecting fluorescent signals; a filter wheel horizontally arranged between the camera and the lens and carrying a plurality of filters corresponding to different emission wavelengths of the fluorescent substances in an annular array; and the motor drives the filter wheel to rotate so as to enable one filter to be positioned right above the lens. However, the fluorescence detection system adopted in most single molecule detection devices at present cannot simultaneously meet detection indexes such as high light path uniformity, large detection field, high resolution and the like, any short plate can directly influence the detection sensitivity and the quantitative accuracy of nucleic acid and protein single molecules, and the development and the application of a single molecule detection technology are greatly limited. Therefore, it is of great significance to develop a new fluorescence detection system.
SUMMERY OF THE UTILITY MODEL
The utility model discloses the technical problem that will solve lies in:
the technical problem that a fluorescence detection system adopted in single molecule detection equipment in the prior art cannot meet detection indexes such as large detection field of view, high resolution and the like at the same time.
The utility model discloses a realize solving above-mentioned technical problem through following technical means:
a fluorescence detection system for a biochip comprises a support and an imaging acquisition assembly vertically arranged on the support, wherein a first optical filter is arranged below the imaging acquisition assembly;
a light source assembly is arranged below the first light filter and comprises a light source rotating disc, the light source rotating disc is coaxial with the imaging acquisition assembly, and a white light source is arranged in the center of the light source rotating disc;
the light source turntable is provided with a plurality of pairs of monochromatic light sources, each pair of monochromatic light sources is symmetrical about the center of the light source turntable, the light emitting ends of the monochromatic light sources point to the position right above the white light source, and the light emitting ends of the monochromatic light sources are provided with second optical filters.
In the utility model, when the fluorescence detection system for the biochip is actually applied, the imaging acquisition assembly is used for imaging a sample to be detected and acquiring a fluorescence signal, the first optical filter can filter the emission fluorescence of a non-characteristic wave band and separate the emission fluorescence of a characteristic wave band; the white light source can provide a bright field environment for focusing and imaging of a sample to be detected; the monochromatic light source can excite the fluorescent substance in the sample to be detected, so that the fluorescent substance can generate a fluorescent signal which can be detected by the optical equipment. The utility model provides a fluorescence detection system for biochip compact structure, greatly reduced the space occupation of hardware, provide the advantage for instrument portability and miniaturized design development; the utility model provides an imaging acquisition assembly is coaxial alternately oblique correlation scheme with white light source, monochromatic source and can effectively reduce the energy loss when light signal transmission, is favorable to waiting to examine the detection of waiting weak fluorescence signal in the sample. The scheme of utilizing the white light source to illuminate the auxiliary imaging acquisition assembly to focus the imaging can provide favorable conditions for high-precision capture of the fluorescence signal in the sample to be detected. The system can simultaneously meet detection indexes such as large detection view field, high resolution and the like. Based on the system, the effective detection of the fluorescence signal in the nucleic acid or protein amplification biochip can be realized.
Preferably, the imaging and collecting assembly comprises a camera and a telecentric lens arranged below the camera, and the telecentric lens faces downwards to the first optical filter and the white light source.
The optical filter turntable driving mechanism is arranged on the support and can drive the optical filter turntable to rotate, the first optical filters are arranged on the plurality of optical filter turntables, all the first optical filters are arranged in a circular array and distributed on the optical filter turntables, and the optical filter turntable driving mechanism can drive the optical filter turntables to drive the first optical filters to rotate to the position below the imaging acquisition assembly.
During practical application, the optical filter turntable driving mechanism drives the optical filter turntable to rotate, and then drives different first optical filters to rotate to the position below the imaging acquisition assembly, so that the multichannel detection requirement can be met.
Preferably, the optical filter turntable driving mechanism adopts a motor, a motor rotating shaft is vertically downward, and the optical filter turntable is arranged on the motor rotating shaft.
Preferably, a groove is formed in the center of the top of the light source rotating disc, and the white light source is located in the groove.
Preferably, the light source assembly further comprises a light source turntable driving mechanism, and the light source turntable driving mechanism can drive the light source turntable to rotate;
the light source turntable driving mechanism adopts a motor, a motor rotating shaft is vertically upward and coaxial with the imaging acquisition assembly, and the light source turntable is installed on the motor rotating shaft.
Preferably, the light source turntable is provided with a plurality of pairs of fixing seats, each pair of fixing seats is symmetrical about the center of the light source turntable, the fixing seats are axially communicated in the fixing seats, the fixing seats are obliquely arranged, the monochromatic light source is arranged at the lower ends of the fixing seats, and the second optical filter is arranged at the upper ends of the fixing seats.
Preferably, the lower end of the fixing seat is provided with a lower fixing seat, and the monochromatic light source is arranged on the lower fixing seat.
Preferably, the bottom of the lower fixed seat is provided with a heat dissipation assembly;
the heat dissipation assembly comprises a plurality of heat dissipation fins arranged in parallel.
Preferably, a light hole coaxial with the fixing seat is arranged in the lower fixing seat, and the light hole is positioned on one side of the light emitting end of the monochromatic light source.
The utility model has the advantages that:
1. in the utility model, when the fluorescence detection system for the biochip is actually applied, the imaging acquisition assembly is used for imaging a sample to be detected and acquiring a fluorescence signal, the first optical filter can filter the emission fluorescence of a non-characteristic wave band and separate the emission fluorescence of a characteristic wave band; the white light source can provide a bright field environment for focusing and imaging the sample to be detected; the monochromatic light source can excite the fluorescent substance in the sample to be detected to enable the fluorescent substance to generate a fluorescent signal which can be detected by optical equipment, the light homogenizing assembly can enhance the uniformity of the optical signal, and the light energy utilization rate is improved. The utility model provides a fluorescence detection system for biochip compact structure, greatly reduces the space occupation of hardware, and provides favorable conditions for instrument portability and miniaturized design and development; the utility model provides an imaging acquisition assembly is coaxial alternately oblique correlation scheme with white light source, monochromatic source and can effectively reduce the energy loss when light signal transmission, is favorable to waiting to examine the detection of waiting weak fluorescence signal in the sample. The scheme of utilizing the white light source to illuminate the auxiliary imaging acquisition assembly to focus the imaging can provide favorable conditions for high-precision capture of the fluorescence signal in the sample to be detected. The system can simultaneously meet detection indexes such as high light path uniformity, large detection view field, high resolution and the like. Based on the system, the effective detection of the fluorescence signal in the nucleic acid or protein amplification biochip can be realized.
2. During practical application, the optical filter turntable driving mechanism drives the optical filter turntable to rotate, and then drives different first optical filters to rotate to the position below the imaging acquisition assembly, so that the multichannel detection requirement can be met.
Drawings
FIGS. 1 and 2 are perspective views (hidden brackets) of different viewing angles of a fluorescence detection system for a biochip according to an embodiment of the present invention;
FIG. 3 is a front view of a fluorescence detection system for a biochip according to an embodiment of the present invention;
FIG. 4 is a right side view of a fluorescence detection system for a biochip according to an embodiment of the present invention;
FIG. 5 isbase:Sub>A sectional view taken along line A-A of FIG. 4;
fig. 6 is a perspective view of a monochromatic light source according to a first embodiment of the present invention;
fig. 7 is a front view of a monochromatic light source according to a first embodiment of the present invention;
FIG. 8 is a cross-sectional view taken along line B-B of FIG. 7;
FIGS. 9 and 10 are exploded views of a fluorescence detection system for a biochip according to an embodiment of the present invention;
wherein, the first and the second end of the pipe are connected with each other,
a bracket-1;
an imaging acquisition assembly-2; a camera-21; a telecentric lens-22;
a first optical filter-3; a filter turntable-31; optical filter turntable driving mechanism-32;
a light source assembly-4; a mounting seat-40; a light source turntable-41; a white light source-42; monochromatic light source-43; a second optical filter-44; dodging assembly-45; a light source turntable driving mechanism-46; a fixed seat-47; a lower fixed seat-48; a heat sink assembly-49; an upper pressing ring-401; aspheric lens-451; fly-eye lens-452; a focusing mirror-453; light hole-481; and a lower pressing ring-482.
Detailed Description
To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the embodiments of the present invention are combined to clearly and completely describe the technical solution in the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
The first embodiment is as follows:
referring to fig. 1-3, a fluorescence detection system for biochip includes a support 1, an image acquisition assembly 2, a first optical filter 3, and a light source assembly 4.
In this embodiment, the main function of the bracket 1 is to provide mounting positions for other parts, and the bracket 1 is not limited to a specific shape as long as the parts can be mounted and matched as required and corresponding functions can be realized. As shown in fig. 3, the camera 21 is mounted on the support 1 through a mounting base, the filter turntable driving mechanism 32 is mounted on the support 1 through a mounting base, and the light source turntable driving mechanism 46 is directly mounted on the support 1.
As shown in fig. 3, the imaging acquisition assembly 2 is vertically arranged on the support 1, and as shown in fig. 5, a first optical filter 3 is arranged below the imaging acquisition assembly 2; as shown in fig. 4 and 5, a light source assembly 4 is arranged below the first optical filter 3, the light source assembly 4 includes a light source turntable 41, the light source turntable 41 is coaxial with the imaging acquisition assembly 2, a white light source 42 is arranged at the center of the light source turntable 41, the white light source 42 is a white light LED, and the irradiation direction is vertical upward and coaxial with the imaging acquisition assembly 2.
Referring to fig. 1 and 5, the light source turntable 41 is provided with a plurality of pairs of monochromatic light sources 43, each pair of monochromatic light sources 43 is symmetric with respect to the center of the light source turntable 41, in this embodiment, 6 pairs of monochromatic light sources 43 are provided, two monochromatic light sources 43 in each pair of monochromatic light sources 43 are the same, light emitting ends of the monochromatic light sources 43 point right above the white light source 42, and light paths are overlapped on the surface of the sample to be detected in a crossed manner, so that the intensity of excitation light can be improved. The center wavelength of the monochromatic light source 43 is one or more of 350nm +/-20 nm, 494nm +/-20 nm, 535nm +/-20 nm, 585nm +/-20 nm, 643nm +/-20 nm and 684nm +/-20 nm.
As shown in fig. 6 and 8, a second optical filter 44 is disposed at a light emitting end of the monochromatic light source 43, the second optical filter 44 is available in the market as the prior art, and a light uniformizing assembly 45 is disposed between the monochromatic light source 43 and the second optical filter 44.
In practical applications, the white light source 42 and the monochromatic light source 43 are not turned on simultaneously, and the white light source 42 is turned on only once before the start of a single experiment; the light paths emitted by a pair of two monochromatic light sources 43 of the same kind are overlapped in the target area of the sample to be detected; each emission filter (i.e., the first filter 3) is in one-to-one correspondence with each monochromatic light source 43, and when the monochromatic light source 43 is turned on, the corresponding emission filter is switched.
Specifically, as shown in fig. 1, the imaging acquisition assembly 2 comprises a camera 21 and a telecentric lens 22 which are sequentially arranged from top to bottom, the camera 21 is used for imaging of a sample to be detected and acquiring a fluorescence signal, and the telecentric lens 22 is used for adjusting the imaging target area. The camera 21 is mounted on the bracket 1 through an L-shaped mounting seat, and the telecentric lens 22 is coaxial with the camera 21 and vertically downward.
As shown in fig. 3, the fluorescence detection system for a biochip further includes an optical filter turntable 31 rotatably mounted on the support 1, an optical filter turntable driving mechanism 32 capable of driving the optical filter turntable 31 to rotate is disposed on the support 1, the number of the first optical filters 3 is several, in this embodiment, six optical filters are respectively in one-to-one correspondence with 6 pairs of monochromatic light sources 43, all the first optical filters 3 are distributed on the optical filter turntable 31 according to a circular array, and the central wavelength and bandwidth of the first optical filters 3 are one or more of 440nm ± 30nm, 518nm ± 30nm, 556nm ± 30nm, 605nm ± 30nm, 667nm ± 30nm, and 710nm ± 30 nm.
The optical filter turntable driving mechanism 32 can drive the optical filter turntable 31 to drive the first optical filter 3 to rotate to the lower part of the imaging acquisition assembly 2, the first optical filter 3 which rotates to the lower part of the imaging acquisition assembly 2 is coaxial with the imaging acquisition assembly 2, different detection channels can be formed between different first optical filters 3 and different pairs of monochromatic light sources 43, and switching can be performed according to selection of the detection channels, so that the emission optical filter (namely, the first optical filter 3) matched with the detection channels is positioned at the tail ends of the camera 21 and the telecentric lens 22.
The specific installation manner of the first optical filter 3 on the optical filter turntable 31 is as follows: six through holes are formed in the optical filter rotating disc 31, and the first optical filters 3 are installed in the corresponding through holes. The first optical filter 3 is available in the prior art.
As shown in fig. 4, the optical filter turntable driving mechanism 32 is a motor, a rotating shaft of the motor is vertically downward, and the optical filter turntable 31 is installed on the rotating shaft of the motor. The motor is arranged on the bracket 1 through an L-shaped mounting seat.
As shown in fig. 3, the light source assembly 4 further includes a light source turntable driving mechanism 46, and the light source turntable driving mechanism 46 can drive the light source turntable 41 to rotate; the light source turntable driving mechanism 46 adopts a motor, the rotating shaft of the motor vertically upwards is coaxial with the imaging acquisition assembly 2, and the light source turntable 41 is installed on the rotating shaft of the motor.
As shown in fig. 1, a plurality of pairs of fixing seats 47 are disposed on the light source turntable 41, each pair of fixing seats 47 is symmetrical with respect to the center of the light source turntable 41, in this embodiment, 12 fixing seats 47 are disposed, the fixing seats 47 correspond to the monochromatic light sources 43 one by one, the specific number of the fixing seats can be increased or decreased according to actual requirements, and the insides of the fixing seats 47 penetrate through along the axial direction thereof and are in a cylindrical structure. Fixing base 47 slope sets up, and fixing base 47's inclination satisfies: the included angle between the axis of the fixed seat 47 and the vertical axes of the camera 21 and the telecentric lens 22 is 30-60 degrees, preferably 47.5 +/-1.5 degrees, and the included angle can be set according to actual requirements in practical application.
As shown in fig. 8, the monochromatic light source 43 is disposed at the lower end of the fixed base 47, the second filter 44 is disposed at the upper end of the fixed base 47, and the light uniformizing assembly 45 is disposed inside the fixed base 47.
Specifically, as shown in fig. 8, the dodging assembly 45 includes an aspheric lens 451, a fly eye lens 452, and a focusing mirror 453, which are sequentially disposed along the light emitting direction of the monochromatic light source 43, and the aspheric lens 451, the fly eye lens 452, and the focusing mirror 453 are all available in the prior art.
As shown in fig. 6-8, a lower fixing seat 48 is provided at the lower end of the fixing seat 47, the upper portion of the lower fixing seat 48 is a cylindrical structure, the lower portion of the lower fixing seat 48 is a square seat, the cylindrical structure at the upper portion of the lower fixing seat 48 is provided with an external thread, an internal thread is provided inside the lower end of the fixing seat 47, and the lower fixing seat 48 is mounted at the lower end of the fixing seat 47 through a thread.
As shown in fig. 8, the monochromatic light source 43 and the aspherical lens 451 are disposed on the lower fixing base 48. Specifically, the lower holder 48 is provided with a light-transmitting hole 481 coaxial with the holder 47, and the light-transmitting hole 481 is located between the monochromatic light source 43 and the aspheric lens 451. Specifically, a conical slope with a large top and a small bottom is arranged above the light hole 481. A lower pressing ring 482 is arranged inside the upper end of the lower fixing seat 48, the lower pressing ring 482 is installed in the lower fixing seat 48 through threads, and the aspheric lens 451 is pressed in the lower fixing seat 48 by the lower pressing ring 482.
As shown in fig. 6 and 8, a heat dissipation assembly 49 is disposed at the bottom of the lower fixing seat 48; as shown in fig. 9 and 10, the heat dissipation assembly 49 includes a plurality of heat dissipation fins arranged in parallel, the upper portion of the heat dissipation assembly 49 is a square base, the heat dissipation fins are uniformly distributed below the square base, the cross-sectional sizes of the square base of the heat dissipation assembly 49 and the square base of the lower fixing base 48 are the same, the two square bases are connected by screws, a groove is formed below the light transmission hole 481 for accommodating the monochromatic light source 43, and the monochromatic light source 43 is fixedly installed in the groove or fixedly connected with the square base of the heat dissipation assembly 49.
As shown in fig. 8, a mounting seat 40 having a cylindrical structure is disposed at an upper end of the fixing seat 47, the fly eye lens 452 is pressed inside the fixing seat 47 by the mounting seat 40, an upper pressing ring 401 is disposed inside an upper end of the mounting seat 40, the upper pressing ring 401 is connected with an inside of an upper end of the mounting seat 40 by a screw thread, the focusing mirror 453 and the second optical filter 44 are pressed inside the mounting seat 40 by the upper pressing ring 401, specifically, an edge portion of the second optical filter 44 protrudes upward and downward, respectively, and after the mounting, a certain gap is formed between the second optical filter 44 and a top portion of the focusing mirror 453.
In this embodiment, the monochromatic light source 43 is a monochromatic LED, the light emitting direction of the monochromatic light source 43 is coaxial with the fixing base 47, and the light-transmitting hole 481, the aspheric lens 451, the fly eye lens 452, the focusing lens 453, the second filter 44, and the fixing base 47 are coaxial.
The working principle is as follows:
in the utility model, when the fluorescence detection system for the biochip is actually applied, the imaging acquisition assembly 2 is used for imaging a sample to be detected and acquiring a fluorescence signal, and the first optical filter 3 can filter the emission fluorescence of a non-characteristic wave band and separate the emission fluorescence of a characteristic wave band; the white light source 42 can provide a bright field environment for focusing and imaging the sample to be detected; the monochromatic light source 43 can excite the fluorescent substance in the sample to be detected, so that the fluorescent substance can generate a fluorescent signal which can be detected by optical equipment, and the light homogenizing assembly 45 can enhance the uniformity of the optical signal and improve the light energy utilization rate. The utility model provides a fluorescence detection system for biochip compact structure, greatly reduced the space occupation of hardware, provide the advantage for instrument portability and miniaturized design development; the utility model provides an imaging acquisition assembly 2 is coaxial alternately oblique correlation scheme with white light source 42, monochromatic light source 43 and can effectively reduce the energy loss when light signal transmission, is favorable to waiting to examine the detection of weak fluorescence signal in the sample. The focusing imaging scheme of the auxiliary imaging acquisition assembly 2 illuminated by the white light source 42 can provide favorable conditions for high-precision capture of fluorescence signals in a sample to be detected. The system can simultaneously meet detection indexes such as high light path uniformity, large detection view field, high resolution and the like. Based on the system, the effective detection of the fluorescence signal in the nucleic acid or protein amplification biochip can be realized.
During practical application, the motor drives the optical filter rotating disc 31 to rotate, so as to drive the different first optical filters 3 to rotate to the lower part of the imaging acquisition assembly 2, and the requirement of multi-channel detection can be met. The light homogenizing assembly 45 formed by the aspheric lens 451, the fly eye lens 452 and the focusing lens 453 can effectively enhance the uniformity of optical signals and improve the utilization rate of light energy.
Example two:
the difference between the present embodiment and the first embodiment is:
the dodging assembly 45 is eliminated, the components such as the aspheric lens 451, the fly eye lens 452, the focusing lens 453 and the lower pressing ring 482 are included, the second filter 44 is directly pressed inside the mounting seat 40 by the upper pressing ring 401, and the rest of the structure is the same as that of the first embodiment.
The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (10)
1. A fluorescence detection system for a biochip, comprising: the device comprises a support (1) and an imaging acquisition assembly (2) vertically arranged on the support (1), wherein a first optical filter (3) is arranged below the imaging acquisition assembly (2);
a light source assembly (4) is arranged below the first optical filter (3), the light source assembly (4) comprises a light source turntable (41), the light source turntable (41) is coaxial with the imaging acquisition assembly (2), and a white light source (42) is arranged at the center of the light source turntable (41);
the light source turntable (41) is provided with a plurality of pairs of monochromatic light sources (43), each pair of monochromatic light sources (43) is centrosymmetric with respect to the light source turntable (41), the light emitting ends of the monochromatic light sources (43) point to the position right above the white light source (42), and the light emitting ends of the monochromatic light sources (43) are provided with second optical filters (44).
2. The fluorescence detection system for biochip according to claim 1, wherein: the imaging acquisition assembly (2) comprises a camera (21) and a telecentric lens (22) arranged below the camera (21), wherein the telecentric lens (22) faces downwards to the first optical filter (3) and the white light source (42).
3. The fluorescence detection system for biochip of claim 1, wherein: still including rotating light filter carousel (31) of installing on support (1), be provided with on support (1) and drive light filter carousel (31) pivoted light filter carousel actuating mechanism (32), first light filter (3) set up in a plurality of, and all first light filters (3) installation circular array distribute on light filter carousel (31), light filter carousel actuating mechanism (32) can drive light filter carousel (31) drive first light filter (3) rotate extremely formation of image acquisition subassembly (2) below.
4. The fluorescence detection system for biochip according to claim 3, wherein: the optical filter turntable driving mechanism (32) adopts a motor, a motor rotating shaft is vertically downward, and the optical filter turntable (31) is installed on the motor rotating shaft.
5. The fluorescence detection system for biochip according to claim 1, wherein: the center of the top of the light source rotating disc (41) is provided with a groove (411), and the white light source (42) is located in the groove (411).
6. The fluorescence detection system for biochip according to claim 1, wherein: the light source assembly (4) further comprises a light source turntable driving mechanism (46), and the light source turntable driving mechanism (46) can drive the light source turntable (41) to rotate;
the light source turntable driving mechanism (46) adopts a motor, the rotating shaft of the motor vertically upwards is coaxial with the imaging acquisition assembly (2), and the light source turntable (41) is installed on the rotating shaft of the motor.
7. The fluorescence detection system for biochip according to claim 1, wherein: the light source turntable (41) is provided with a plurality of pairs of fixing seats (47), each pair of fixing seats (47) is centrosymmetric relative to the light source turntable (41), the fixing seats (47) are communicated along the axial direction, the fixing seats (47) are obliquely arranged, the monochromatic light source (43) is arranged at the lower end of each fixing seat (47), and the second light filter (44) is arranged at the upper end of each fixing seat (47).
8. The fluorescence detection system for biochip according to claim 7, wherein: the lower end of the fixed seat (47) is provided with a lower fixed seat (48), and the monochromatic light source (43) is arranged on the lower fixed seat (48).
9. The fluorescence detection system for biochip according to claim 8, wherein: a heat dissipation assembly (49) is arranged at the bottom of the lower fixed seat (48);
the heat dissipation assembly (49) comprises a plurality of heat dissipation fins which are arranged in parallel.
10. The fluorescence detection system for biochip according to claim 8, wherein: the lower fixing seat (48) is internally provided with a light hole (481) which is coaxial with the fixing seat (47), and the light hole (481) is positioned on one side of the light emitting end of the monochromatic light source (43).
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