CN217733146U - Micro-fluidic culture system for organoid - Google Patents
Micro-fluidic culture system for organoid Download PDFInfo
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- CN217733146U CN217733146U CN202221870094.4U CN202221870094U CN217733146U CN 217733146 U CN217733146 U CN 217733146U CN 202221870094 U CN202221870094 U CN 202221870094U CN 217733146 U CN217733146 U CN 217733146U
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Abstract
The utility model discloses a micro-fluidic culture system of organoid includes into liquid centrifuge bottle, peristaltic pump, culture plate, play liquid centrifuge bottle along the liquid flow direction. The system provided by the utility model can be used to the cultivation of conventional organoid, through providing the culture medium with miniflow's mode, can make the organoid in time obtain nutrient substance and get rid of metabolic waste to the growth of giving the organoid provides a more and is close internal cultivation environment. The utility model provides an organoid micro-fluidic culture system is simple, and is reliable, can satisfy different field researchers to the demand that organoid cultivateed, accords with the development direction of biological materials science and cell biology, has fine popularization and application and worth.
Description
Technical Field
The utility model relates to a tissue engineering and biotechnology field especially relate to a class organ micro-fluidic culture system.
Background
Nowadays, organoid culture methods have been established in various tissues, such as small intestine, brain, liver, pancreas, stomach, etc., since organoids have the ability of propagation and passage in vitro similar to cell lines, genetic manipulations such as RNAi, viral infection, gene knockout, etc. can be performed as well, and furthermore, since organoids have a certain organoid structure, they can better reflect physiological processes in vivo, and thus they are a powerful tool for in vitro studies, especially in studies involving humans, organoids established from human sources have more irreplaceable effects (Kim et al, 2020).
At present, the most common organoid culture method is to culture in a cell well plate by means of Matrigel embedding, changing the culture solution and subculturing. Organoid fluid changes are often between 3-5 days, and conventional fluid changes have problems with metabolic waste accumulation, and oxygen consumption, which can hinder organoid growth to some extent (Garreta et al, 2021). The introduction of micro-fluidic means would greatly ameliorate this problem and the development of organoid micro-fluidic culture devices would provide a more in vivo-like microenvironment for organoid culture.
How to solve the above technical problems is the subject faced by the present invention.
SUMMERY OF THE UTILITY MODEL
In order to solve the not enough of prior art, the utility model provides a reasonable in design, simple structure, safe and reliable, sustainability provides fresh culture medium, effectively avoids the metabolism waste accumulation and the organoid micro-fluidic culture system of oxygen consumption problem that the tradition mode of changing liquid caused for the organoid of cultivateing.
The utility model provides a technical scheme that its technical problem adopted is: the utility model provides a micro-fluidic culture system of organoid, include into liquid centrifuge bottle and play liquid centrifuge bottle, it is connected with the peristaltic pump to go into liquid centrifuge bottle, the peristaltic pump with be connected with the culture plate between the play liquid centrifuge bottle.
Rubber stoppers are arranged at openings at the tops of the liquid inlet centrifugal bottle and the liquid outlet centrifugal bottle;
go into liquid centrifuge bottle's rubber buffer department through rubber conduit and incubator air intercommunication, go into liquid centrifuge bottle's rubber buffer department through the pipe with the input of peristaltic pump is connected.
Communicating holes are formed between every two adjacent culture holes in the liquid flowing direction of the culture plate, and liquid inlet holes are formed in the joints of the culture plate and the peristaltic pumps;
the connection part of the culture plate and the liquid outlet centrifugal bottle is provided with a liquid outlet hole, and the liquid inlet hole, the communicating hole and the liquid outlet hole are correspondingly formed.
Every go into liquid hole, the intercommunicating pore with go out the liquid hole and all set up in the cultivation hole pore wall upper portion of cultivateing the board, and the aperture is 0.3-0.5cm, cultivateing the board and can replacing for the blake bottle, or the cultivateing board of different hole numbers, all be provided with and go into liquid hole, play liquid hole and the intercommunicating pore of corresponding quantity.
The liquid inlet hole of the culture plate is connected with a liquid inlet guide pipe, and the liquid outlet hole of the culture plate is connected with a liquid outlet guide pipe;
the liquid inlet pipe is connected with the output end of the peristaltic pump, and the liquid outlet pipe is connected with the rubber plug of the liquid outlet centrifugal bottle and communicated with the inside of the liquid outlet centrifugal bottle.
The rubber stopper of the liquid outlet centrifugal bottle is communicated with the air in the incubator through a rubber conduit.
The whole set of device is sterilized before connection and placed in an incubator after communication.
The utility model has the advantages that: simple structure, reasonable in design, the utility model provides a system can be used to carry out the micro-fluidic of organoid and cultivates, provides fresh culture medium through the organoid that gives the cultivation sustainability, will avoid the tradition to trade metabolic waste accumulation and the oxygen consumption problem that the liquid mode caused, provides a growth environment that is more close internally for the growth of organoid. The utility model discloses a organoid micro-fluidic culture system use is simple and convenient, and is reliable, can satisfy the demand that different field researchers three-dimensional cultivateed, accords with the development direction of biological materials science and cell biology, has fine popularization and application and worth.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic view of the three-dimensional structure of the culture plate of the present invention.
Wherein the reference numerals are: 1. a liquid inlet centrifugal bottle; 2. a peristaltic pump; 3. a fluid inlet conduit; 4. culturing the plate; 5. an organoid; 6. a liquid outlet conduit; 7. discharging liquid from a centrifugal bottle; 8. a liquid inlet hole; 9. a culture well; 10. a communicating hole; 11. and a liquid outlet hole.
Detailed Description
Technical features of the present solution can be clearly explained, and the present solution is explained by the following detailed description.
The first embodiment is as follows:
referring to fig. 1 to 2, this embodiment is a kind of organ microfluidic culture system, including income liquid centrifuge bottle 1 and play liquid centrifuge bottle 7, it is 500ml centrifuge bottles to go into liquid centrifuge bottle 1 and play liquid centrifuge bottle 7, and top opening department is provided with the rubber buffer, goes into the rubber buffer department of liquid centrifuge bottle 1 and communicates with incubator air through the rubber pipe, goes into liquid centrifuge bottle 1 and is connected with peristaltic pump 2, goes into the rubber buffer department of liquid centrifuge bottle 1 and is connected with the input of peristaltic pump 2 through the pipe.
The culture plate 4 is connected between the peristaltic pump 2 and the liquid outlet centrifuge bottle 7, the organoid 5 is positioned in culture holes 9 of the culture plate 4, the culture plate 4 is a six-hole plate, communication holes 10 are arranged between every two adjacent culture holes 9 in the liquid flowing direction of the culture plate 4, 4 communication holes 10 are arranged, liquid inlet holes 8 are arranged at the joints of the culture plate 4 and the peristaltic pump 2, two liquid inlet holes 8 are arranged corresponding to the culture holes 9 of the culture plate 4, two liquid outlet holes 11 are arranged at the joints of the culture plate 4 and the liquid outlet centrifuge bottle 7, two liquid outlet holes 11 are arranged corresponding to the culture holes 9 of the culture plate 4, the liquid inlet holes 8, the communication holes 10 and the liquid outlet holes 11 are arranged corresponding to form two groups of channels, each liquid inlet hole 8, the communication holes 10 and the liquid outlet holes 11 are arranged at the upper parts of the culture hole walls of the culture plate 4, the hole diameters are 0.3cm, liquid inlet guide pipes 3 are connected to the liquid outlet holes 8 of the culture plate 4, the liquid outlet guide pipes 6 are connected to the liquid outlet pipes 3, the liquid inlet guide pipes 3 are connected to the output ends of the pump 2, and the rubber plugs are connected to the rubber plugs at the positions of the centrifuge bottle 7.
The rubber stopper of the liquid outlet centrifugal bottle 7 is communicated with the air in the incubator through a rubber conduit, and the whole set of device is sterilized correspondingly before being connected and then is placed in the incubator after being communicated.
Example two:
referring to fig. 1 to 2, this embodiment is a kind of organ microfluidic culture system, including income liquid centrifuge bottle 1 and play liquid centrifuge bottle 7, it is 500ml centrifuge bottles to go into liquid centrifuge bottle 1 and play liquid centrifuge bottle 7, and top opening department is provided with the rubber buffer, goes into the rubber buffer department of liquid centrifuge bottle 1 and communicates with incubator air through the rubber pipe, goes into liquid centrifuge bottle 1 and is connected with peristaltic pump 2, goes into the rubber buffer department of liquid centrifuge bottle 1 and is connected with the input of peristaltic pump 2 through the pipe.
The culture plate 4 is connected between the peristaltic pump 2 and the liquid outlet centrifuge bottle 7, the organoid 5 is positioned in culture holes 9 of the culture plate 4, the culture plate 4 is a six-hole plate, communication holes 10 are arranged between every two adjacent culture holes 9 in the liquid flowing direction of the culture plate 4, 4 communication holes 10 are arranged, liquid inlet holes 8 are arranged at the joints of the culture plate 4 and the peristaltic pump 2, two liquid inlet holes 8 are arranged corresponding to the culture holes 9 of the culture plate 4, two liquid outlet holes 11 are arranged at the joints of the culture plate 4 and the liquid outlet centrifuge bottle 7, two liquid outlet holes 11 are arranged corresponding to the culture holes 9 of the culture plate 4, the liquid inlet holes 8, the communication holes 10 and the liquid outlet holes 11 are arranged corresponding to form two groups of channels, each liquid inlet hole 8, the communication hole 10 and the liquid outlet hole 11 are arranged at the upper part of the wall of the culture hole of the culture plate 4, the aperture is 0.5cm, the liquid inlet hole 8 of the culture plate 4 is connected with a liquid inlet conduit 3, the liquid outlet hole 11 of the culture plate 4 is connected with a liquid outlet conduit 6, the liquid outlet conduit 3 is connected with the output end of the pump 2, and the conduit 6 is connected with the rubber plug 7 of the centrifuge bottle 7.
The rubber stopper of the liquid outlet centrifugal bottle 7 is communicated with the air in the incubator through a rubber conduit, and the whole set of device is sterilized correspondingly before being connected and then is placed in the incubator after being communicated.
The technical features that the utility model has not been described can be realized through or adopt prior art, and no longer give unnecessary details here, and of course, the above-mentioned explanation is not right the utility model discloses a restriction, the utility model discloses also not only be limited to the above-mentioned example, ordinary skilled person in this technical field is in the utility model discloses a change, modification, interpolation or replacement made in the essential scope also should belong to the utility model discloses a protection scope.
Claims (6)
1. The organoid microfluidic culture system is characterized by comprising a liquid inlet centrifugal bottle (1) and a liquid outlet centrifugal bottle (7), wherein the liquid inlet centrifugal bottle (1) is connected with a peristaltic pump (2), and a culture plate (4) is connected between the peristaltic pump (2) and the liquid outlet centrifugal bottle (7).
2. The organoid microfluidic culture system of claim 1, wherein rubber stoppers are provided at openings at the top of the inlet centrifuge bottle (1) and the outlet centrifuge bottle (7);
go into the rubber buffer department of liquid centrifuge bottle (1) through rubber pipe and incubator interior air intercommunication, the rubber buffer department of going into liquid centrifuge bottle (1) through the pipe with the input of peristaltic pump (2) is connected.
3. The organoid microfluidic culture system of claim 1, wherein a communication hole (10) is provided between each two adjacent culture wells (9) in the direction of liquid flow of the culture plate (4);
a liquid inlet hole (8) is formed in the joint of the culture plate (4) and the peristaltic pump (2), a liquid outlet hole (11) is formed in the joint of the culture plate (4) and the liquid outlet centrifugal bottle (7), and the liquid inlet hole (8) and the communicating hole (10) are formed in the corresponding position of the liquid outlet hole (11).
4. The organoid microfluidic culture system of claim 3, wherein each of the inlet well (8), the communication hole (10) and the outlet well (11) is opened on the upper part of the wall of the culture well of the culture plate (4) and has a pore size of 0.3-0.5cm.
5. The organoid microfluidic culture system of claim 3, wherein the plate (4) is connected to a liquid inlet conduit (3) at a liquid inlet (8) and a liquid outlet conduit (6) at a liquid outlet (11) of the plate (4);
go into liquid pipe (3) and connect peristaltic pump (2) output, go out liquid pipe (6) and connect go out the rubber buffer department of liquid centrifuge bottle (7) with go out the inside intercommunication of liquid centrifuge bottle (7).
6. The organoid microfluidic culture system of claim 1, wherein the rubber stopper of the tapping centrifuge bottle (7) is in air communication with the inside of the incubator through a rubber conduit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221870094.4U CN217733146U (en) | 2022-07-08 | 2022-07-08 | Micro-fluidic culture system for organoid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221870094.4U CN217733146U (en) | 2022-07-08 | 2022-07-08 | Micro-fluidic culture system for organoid |
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| Publication Number | Publication Date |
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| CN217733146U true CN217733146U (en) | 2022-11-04 |
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| CN202221870094.4U Active CN217733146U (en) | 2022-07-08 | 2022-07-08 | Micro-fluidic culture system for organoid |
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- 2022-07-08 CN CN202221870094.4U patent/CN217733146U/en active Active
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