CN217561520U - Detection card, colloidal gold-chemiluminescence detector - Google Patents

Abstract

The utility model belongs to the immunodetection field discloses detection card, colloidal gold-chemiluminescence detector. The utility model discloses a detection card includes test paper strip and casing, and test paper strip is including the sample pad, combination pad, reaction pad and the pad that connect gradually, can detect up to 12 kinds of analytes to be examined on the reaction pad of test paper strip in the detection card simultaneously, and the casing contains 2 ~ 12 draw-in grooves again, can insert a plurality of test paper strips simultaneously, and the test paper strip in each draw-in groove can detect the analytes to be examined of different concentrations, has realized that a detection card can once only detect a plurality kinds, the analytes to be examined of different concentrations; the colloidal gold-chemiluminescence detector is matched, the instrument structure is simple, the detection cost can be reduced, the detection time can be shortened, and large-scale qualitative and quantitative detection can be realized.

Description

Detection card, colloidal gold-chemiluminescence detector

Technical Field

The utility model belongs to the immunodetection field, concretely relates to detects card, colloidal gold-chemiluminescence detector.

Background

The detection of antibiotics is of great importance in many fields. For example, in clinical applications, by monitoring the concentration of antibiotics in the blood, maximal therapeutic effect is achieved and side effects are minimized; in agriculture, antibiotic residues in animal derived food can cause allergies or poisoning to humans; mycotoxins are important sources of food-borne diseases and can cause acute toxicity, mutagenesis and carcinogenesis. Therefore, it is important to ensure food safety, and the methods commonly used for food detection are gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and enzyme-linked immunoassay, which are complicated and time-consuming to operate, require expensive equipment and specialized operators, and are limited to laboratories with complete equipment. Therefore, development of a novel detection apparatus is urgently required.

The lateral flow immunoassay (LFA) method has been widely used in the field of antibiotic and mycotoxin detection as a rapid, simple, and economical detection and analysis method. The sensitivity and accuracy of LFA mainly depend on signal labels, colloidal gold is the most widely used nano label, but the sensitivity is low, quantum dots, magnetic nano particles and up-conversion nano particles are widely used on LFA as substitute labels of gold nano particles, and when the nano materials are combined with monoclonal antibodies, organic cross-linking agents (such as ethyl carbodiimide hydrochloride, N-hydroxysuccinimide and glutaraldehyde) are needed, so that the activity of the monoclonal antibodies is reduced, and the sensitivity of the monoclonal antibodies is affected.

At present, LFA can be used for detecting antibiotics and mycotoxins in food, and a common means is to use a traditional colloidal gold nano label to qualitatively detect one or more analytes or use a fluorescent nano label to quantitatively detect one or two target analytes, but most of devices using fluorescent quantitative detection can only detect one test strip at the same time, which is not beneficial to simultaneously detecting a plurality of residual antibiotics and mycotoxins in food.

Therefore, it is important to develop an instrument which has a small volume, a simple structure and a simple operation and can simultaneously detect a plurality of substances to be detected.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the above-mentioned prior art at least. Therefore, the utility model provides a detect card can detect the multiple analyte of waiting to examine simultaneously.

The utility model also provides a colloidal gold-chemiluminescence detector for with detect the card and take to wait to examine the analyte and detect, not only can reduce the detection cost, shorten check-out time, can realize large-scale detection moreover.

According to the utility model discloses an aspect provides the detection card, include:

the detection test strip comprises a sample pad, a combination pad, a reaction pad and a water absorption pad which are sequentially connected, wherein 1-12 detection lines are arranged on the reaction pad;

the device comprises a shell, wherein a sample adding window and a visual window are arranged on the surface of the shell, 2-12 clamping grooves are arranged in the shell, and the distance between every two adjacent clamping grooves is 1-3 mm; the casing is configured as when the test strip is fixed in the draw-in groove, the application of sample window with the sample pad corresponds, and the visual window with the reaction pad corresponds.

According to the utility model discloses a preferred embodiment has following beneficial effect at least:

the utility model discloses an among the detection card, can set up simultaneously on the reaction pad of test paper strip and reach 12 detection lines, can set up one kind on every detection line and wait to examine the antigen of analyte, can detect simultaneously every test paper strip promptly and wait to examine the analyte by up to 12. The casing contains 2 ~ 12 draw-in grooves again, can insert a plurality of above-mentioned test paper strips simultaneously, and the test paper strip in every draw-in groove can detect the analyte of waiting to examine of different concentration, has realized that a test card can once only detect the analyte of waiting to examine of multiple, different concentrations.

In some embodiments of the present invention, the reaction pad is provided with a detection line and a control line.

In some embodiments of the invention, the conjugate pad is dispensed with an analyte antibody-gold colloid-enzyme complex to be detected.

In some embodiments of the invention, the enzyme is selected from any one of horseradish peroxidase (HRP) or alkaline phosphatase (ALP).

In some preferred embodiments of the invention, the enzyme is selected from horseradish peroxidase.

In some embodiments of the present invention, the detection line is distributed with an antigen of an analyte to be detected.

In some embodiments of the invention, the sample pad is disposed on the conjugate pad.

In some embodiments of the present invention, the combination pad is close to the one end overlap joint of the reaction pad is in the one end of the reaction pad, the water absorption pad is close to the one end overlap joint of the reaction pad is in the other end of the reaction pad.

In some embodiments of the present invention, the length of the bonding pad overlapped with the reaction pad is 1-2 mm.

In some preferred embodiments of the present invention, the length of the bonding pad overlapping the reaction pad is 1mm.

In some embodiments of the present invention, the length of the water absorption pad overlapped with the reaction pad is 1-2 mm.

In some preferred embodiments of the present invention, the absorbent pad overlaps the reaction pad by 1mm.

In some embodiments of the invention, the reaction pad is selected from any one of a Nitrocellulose (NC) membrane, a polyvinylidene fluoride (PVDF) membrane, or a nylon membrane.

In some preferred embodiments of the present invention, the reaction pad is a nitrocellulose membrane.

In some embodiments of the present invention, the application window corresponds to the sample pad, the visual window corresponds to the reaction pad, that is, the detection strip is fixed in the slot, the accessible the hole of the application window adds the sample to the sample pad of the detection strip, the reaction pad is provided with the detection line and the reaction result accessible of the control line, the visual window is used for observation and capture.

The utility model discloses an in some embodiments, follow the application of sample window adds waits to examine the compound liquid of analyte, wait to examine the compound liquid of analyte and reach during the gasket, with wait to examine the combination of analyte antibody-colloidal gold-enzyme complex on the gasket, not with wait to examine that the compound liquid of analyte combines it arrives to examine analyte antibody-colloidal gold-enzyme complex flows to the reaction pad, with the antigen binding of waiting to examine the analyte of distribution on the detection line of reaction pad, the colour depth of colloidal gold and the quantity accumulation accessible of enzyme the visual window presents.

In some embodiments of the present invention, the analyte complex fluid to be detected is a sample including a plurality of analytes to be detected.

According to a second aspect of the present invention, there is provided a colloidal gold-chemiluminescence detector applied to the detection card, comprising:

the object stage is used for fixing the detection card;

the light-emitting capturing element is internally provided with a charge-coupled device, the charge-coupled device is used for capturing a colloidal gold signal and a chemiluminescence signal on the detection card, and the charge-coupled device is vertical to the objective table;

wherein the stage and the charge coupled device are configured to be capable of relative movement to change a signal capture range of the charge coupled device to the test card.

According to a preferred embodiment of the present invention, at least the following advantages are provided:

the colloidal gold-chemiluminescence detector of the utility model is used with a detection card, wherein the charge coupled device and the objective table are vertically arranged, but not parallelly arranged, and the reflector is used for refraction imaging, so that the charge coupled device can enlarge and reduce the capture area of the detection card through the relative movement of the objective table and the charge coupled device according to the size of the detection card; the charge coupled device can capture a colloidal gold signal and a chemiluminescence signal, wherein the color depth of the colloidal gold signal can be used as a qualitative basis, the chemiluminescence signal can be used as a quantitative basis, qualitative and quantitative multiple detection of an analyte to be detected is realized, the instrument result is simple, the detection cost can be reduced, the detection time is shortened, and large-scale detection can be realized.

In some embodiments of the invention, the luminescent capture element is any one of a camera or a video camera.

In some embodiments of the present invention, the carrier can hold at most 2 detection cards, and the relative movement between the carrier and the ccd adjusts the ccd to the signal capture range of the detection card, so that 2-220 analytes to be detected can be detected.

In some embodiments of the invention, the colloidal gold signal is captured in a lighted environment.

In some embodiments of the invention, the chemiluminescent signal is captured in a light-tight environment.

In some embodiments of the present invention, a light source is disposed on the light-emitting capturing element, and when the light source is in an on state, the ccd is used for capturing the colloidal gold signal; the charge coupled device is used for capturing the chemiluminescence signal when the light source is in an off state.

In some embodiments of the invention, the light capture element is configured with a lens, and the light source is disposed on the lens.

In some preferred embodiments of the present invention, the charge coupled device is vertically disposed above the stage.

In some embodiments of the present invention, the colloidal gold-chemiluminescence detector further comprises a computer and a display, the computer is connected to the display, and the computer analyzes the colloidal gold signal and the chemiluminescence signal, and transmits an analysis result to the display, thereby reading an image result and a data analysis result from the display.

In some preferred embodiments of the present invention, the stage is disposed in a box, which provides a light-tight environment.

In some preferred embodiments of the present invention, the case is any one of a cube or a rectangular parallelepiped.

In some preferred embodiments of the present invention, the upper surface of the box is provided with a hole for inserting the lens of the light-emitting capture element, so that the light-emitting capture element is vertically disposed above the box, and at this time, the charge coupled device is also vertically disposed above the box.

In some preferred embodiments of the present invention, an LED lamp is disposed on the lens, and when the LED lamp is turned on, the LED lamp is used to provide a light source to assist the charge-coupled device to capture the colloidal gold signal; the charge coupled device is used for capturing the chemiluminescence signal when the LED lamp is turned off.

In some preferred embodiments of the present invention, the LED lamp is disposed on a lamp holder, and the lamp holder surrounds the lens or is disposed at one end of the lens.

In some preferred embodiments of the present invention, the lamp holder is disposed around the lens in a circular or U-shaped manner.

Drawings

The invention is further described with reference to the following figures and examples, in which:

fig. 1 is a schematic structural diagram of a detection card and a colloidal gold-chemiluminescence detector according to embodiment 1 of the present invention;

fig. 2 is a schematic structural diagram of a test strip in embodiment 1 of the present invention;

fig. 3 is a schematic view of a housing structure of a detection card in embodiment 1 of the present invention;

FIG. 4 is a graph showing the results of the colloidal gold signal and chemiluminescence signal capture in example 2 of the present invention; wherein A is a colloidal gold signal capture result, and B is a chemiluminescent signal capture result;

FIG. 5 is a diagram showing the results of quantitative measurements of 6 analytes to be detected using chemiluminescence mode in example 2 of the present invention; wherein A is Ochratoxin (OTA), B is Thiamphenicol (TAP), C is Zearalenone (ZEN), D is vomitoxin (DON), E is Spectinomycin (SM), and F is Erythromycin (ERM);

FIG. 6 is a graph showing the results of quantitative measurements of 4 analytes to be detected using a chemiluminescence mode in example 2 of the present invention; wherein G is Enrofloxacin (ENR), H is Tetracycline (TC), I is fumonisin (FB 1), and J is Aflatoxin (AF);

FIG. 7 is a diagram showing the results of capturing the colloidal gold signals and the chemiluminescent signals of 220 analytes to be detected in example 3 of the present invention; wherein the upper is the result of capturing colloidal gold signal, and the lower is the result of capturing chemiluminescence signal.

Reference numerals:

110-test card; 111-sample pad; 112-a conjugate pad; 113-a reaction pad; 114-absorbent pad; 115-detection lines; 116-control line; 120-mounting a clamping shell; 121-lower clamping shell; 122-a card slot; 123-a sample application window; 124-visible window; 210-an object stage; 211-a box body; 212-a door; 213-luminescence capture element; 214-lens; 215-circular lamp holder; 216-a computer; 217-display.

Detailed Description

Reference will now be made in detail to the embodiments of the present invention, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The following description of the embodiments is merely exemplary in nature and is in no way intended to limit the invention.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, etc., is the orientation or positional relationship shown on the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, unless there is an explicit limitation, the words such as setting and connection should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meaning of the above words in the present invention by combining the specific contents of the technical solution.

Reference throughout the specification to "one embodiment," "some embodiments," or similar language means that a particular feature, structure, material, or characteristic described in connection with the embodiment is included in at least one embodiment of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments.

The test methods used in the examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are commercially available reagents and materials unless otherwise specified.

Example 1

This example designs a test card and a colloidal gold-chemiluminescence tester, and is described with reference to fig. 1, 2, and 3.

The test card 110 includes a test strip and a housing.

The structure schematic diagram of the test strip is shown in fig. 2, and the test strip includes a sample pad 111, a binding pad 112, a reaction pad 113, and a water absorption pad 114, which are connected in sequence. The sample pad 111 is arranged on the combination pad 112, one end of the combination pad 112 close to the reaction pad 113 is lapped at the left end of the reaction pad 113, the lapping length is 1mm, an analyte antibody-colloidal gold-enzyme complex to be detected is distributed on the combination pad 112, and the enzyme is selected from HRP; one end of the water absorption pad 114 close to the reaction pad 113 is lapped at the right end of the reaction pad 113, and the lapping length is 1mm; the reaction pad 113 is an NC membrane, on which 10 detection lines 115 and 1 control line 116 are disposed, antigens of analytes to be detected are distributed on the detection lines 115, the antigens of the analytes to be detected on the 10 detection lines are different, the distance between the 10 detection lines 115 is 1mm, and the distance between the last detection line 115 and the control line is 1mm.

As shown in fig. 3, the test card 110 further includes a housing, the housing includes an upper housing 120 and a lower housing 121, the upper housing 120 and the lower housing 121 cover to form a housing containing 12 card slots 122, the test strips are disposed in the card slots 122, and one test strip is disposed in each card slot 122; the upper card shell 120 is provided with an adding window 123 and a visible window 124, the adding window 123 corresponds to the sample pad 111 of the detection test strip, the visible window 124 corresponds to the reaction pad 113 of the detection test strip, generally, the compound liquid of the analyte to be detected is added from the adding window 123, when the compound liquid of the analyte to be detected reaches the combination pad 112, the compound liquid of the analyte to be detected is combined with the antibody-colloidal gold-enzyme compound of the analyte to be detected on the combination pad 112, the antibody-colloidal gold-enzyme compound of the analyte to be detected which is not combined with the compound liquid of the analyte to be detected flows to the reaction pad 113, and is combined with the antigen of the analyte to be detected distributed on the detection line 115 of the reaction pad 113, and the color depth of the colloidal gold and the quantity accumulation of the enzyme can be presented through the visible window 124.

Referring to fig. 1, the gold colloid-chemiluminescence detector includes a stage 210, a housing 211, a luminescence capture element 213, a computer 216, and a display 217. The object stage 210 is arranged in the box body 211, the box body 211 is a cuboid, a door 212 is arranged in front of the box body 211, the door 212 can be opened or closed, the detection card 110 is placed on the object stage 210 by opening the door 212, and a light-shading environment can be provided when the door 212 is closed; the upper surface of the housing 211 is provided with a hole (not shown) for inserting the lens 214 of the light capture element 213, so that the light capture element 213 is vertically disposed above the housing 211, and the ccd (disposed in the light capture element 213 and not shown) is also vertically disposed above the housing 211, where the light capture element 213 is configured as a camera; the stage 210 is configured as a lifting stage, that is, the distance between the detection card 110 and the ccd is adjusted by moving up and down in a direction perpendicular to the ccd, so as to adjust the signal capture range of the ccd on the detection card 110; a circular lamp holder 215 is arranged around the lens 214, the arm end of the circular lamp holder 215 is fixed at the rear of the box body 211 through screws, and then the LED lamp (not shown in the figure) is surrounded on the circular lamp holder 215; the LED lamp is used for providing a light source when being turned on, assisting the charge coupled device to capture the colloidal gold signal, and the LED lamp is turned off, namely when the detection card 110 is in a light-shading environment, the charge coupled device is used for capturing a chemiluminescence signal; the computer 216 is connected to the display 217 and the luminescence capture element 213, respectively, the colloidal gold signal and the chemiluminescence signal captured by the charge-coupled device in the luminescence capture element 213 are transmitted to the computer 216, the computer 216 analyzes the colloidal gold signal and the chemiluminescence signal, transmits the analysis result to the display 217, and visually reads the image result and the data analysis result through the display 217.

Example 2

In this example, 5 commercial antibiotics and 5 commercial mycotoxins were detected, and the specific process is as follows:

separately taking the corresponding antibody-colloidal gold-HRP conjugates for each antibiotic and each mycotoxin in a test tube to form a mixture of 10 Ab-AuNPs-HRP conjugates, wherein Spectinomycin (SM) -AuNPs-HRP 3. Mu.L, enrofloxacin (ENR) -AuNPs-HRP 2. Mu.L, thiamphenicol (TAP) -AuNPs-HRP 1. Mu.L, tetracycline (TC) -AuNPs-HRP 1. Mu.L, erythromycin (ERM) -AuNPs-HRP 1. Mu.L, zearalenone (ZEN) -AuNPs-HRP 1. Mu.L, vomitoxin (DON) -AuNPs-HRP 1. Mu.L, ochratoxin (OTA) -AuNPs-HRP 1. Mu.L, aufumonisin (FB 1) -AuNPs-HRP 1. Mu.L and aflatoxin (HRP) -NPs-1. Mu.L, 13 mul, adding 13 mul Ab-AuNPs-HRP conjugate complex liquid on the binding pad 112, distributing antigen liquid of analytes to be detected on the detection line 115, distributing secondary antibody of goat anti-mouse on the control line, adding the above-mentioned 10 kinds of complex liquid of analytes to be detected from the sample adding window 123 to the sample pad 111, wherein the concentrations of the complex liquid of analytes to be detected added in each sample adding window are different, namely the concentrations of the complex liquid of analytes to be detected on 10 detection test strips are different and are respectively 0.01, 0.05, 0.5, 1, 2, 5, 10, 20, 50 and 100ng/mL, opening the door 212 of the box body 211, and placing the detection card 110 on the object stage 210 in the box body 211;

the software for turning on the display 217, clicking on the turn-on device, activates the light-emitting capture element 213, sets the type of operation of the charge-coupled device: selecting colloid Jin Xuanxiang, and turning on an LED lamp to capture a colloidal gold signal; not selecting colloid Jin Xuanxiang, capturing a chemiluminescent signal by default, turning off an LED lamp, capturing a colloidal gold signal, taking out the detection card 110 after capturing is finished, adding a chemiluminescent substrate to a sample pad 111 of the detection test strip through a sample adding window 123, placing the detection card 110 on a carrying platform in a box body 211, and capturing the chemiluminescent signal in a switching mode; setting a filtering threshold value for secondary filtering of background noise, independently setting judgment threshold values and exposure time of 10 analytes, then setting temperature and grid parameters, performing dark field calibration on a photographing system, clicking to start, acquiring a set number of pictures, calculating and calibrating after the acquisition is completed, and simultaneously generating a calibration result, wherein the pictures and data of the detection result are displayed on the display 217.

The capture results of the gold colloid signal and the chemiluminescence signal are shown in fig. 4, in which the first row of numbers represents the concentration gradient of the analyte to be detected, the C-line in the left column represents the control line, and the 10 analytes under the control line are the analytes to be detected. FIG. 4A is a diagram of a colloidal gold signal, and B is a diagram of a chemiluminescent signal, which shows that when the concentration of the analyte to be detected is 0.01ng/mL, the colloidal gold signal and the chemiluminescent signal of 10 detection lines 115 and 1 control line 116 on the test strip can be displayed, because the antibody of the analyte to be detected on the binding pad 112 is sufficient to react with the analyte to be detected; with the increase of the concentration of the analyte to be detected, the number of the detection lines 115 is reduced, and when the concentration reaches 100ng/mL, the detection lines 115 are completely disappeared, because the concentration of the analyte to be detected is far greater than the concentration of the antibody of the analyte to be detected on the binding pad 112, the antibody of the analyte to be detected cannot be bound with the antigen of the analyte to be detected distributed on the detection lines 115, i.e., no corresponding colloidal gold or enzyme label exists, and no corresponding colloidal gold signal or chemiluminescence signal exists at the position of the detection lines 115.

Meanwhile, the analyte to be detected was quantitatively detected using the chemiluminescence mode, and the results are shown in fig. 5 and 6. FIGS. 5 and 6 show competitive inhibition curves of 10 analytes to be detected and a linear regression equation of a certain concentration range, wherein the competitive inhibition curves show (detection line/control line, T/C in the graph) that the chemiluminescence intensity changes along with the change of the concentration of the analytes to be detected, the linear regression equation shows (detection line/control line, T/C in the graph) that the chemiluminescence intensity changes along with the change of the concentration of the analytes to be detected after taking logarithm, and the results show that the chemiluminescence intensity of the detection line/control line decreases along with the increase of the concentration of the analytes to be detected, and the competitive inhibition curves and the linear regression equation have the same trend and are consistent with the result graphs of colloidal gold signals and chemiluminescence signalsAnd R is ² All are greater than 0.95, show that the stability, the repeatability of test paper strip and colloidal gold-chemiluminescence detector in the utility model are all better.

Example 3

The present embodiment detects 220 analytes to be detected, and the specific process is the same as that of embodiment 2, except that 2 detection cards 110 are placed on the stage 210, and the stage 210 is moved up and down to adjust the signal capture range when capturing signals. As a result, as shown in FIG. 7, the CCD device can detect 220 analytes to be detected at most. This is because 2 detection cards 110 can be placed on the object stage 210 at most, 12 detection strips are respectively disposed on two detection cards 110, 10 detection lines 115 are disposed on each detection strip, and by moving the object stage 210 up and down, at most, all analytes to be detected (i.e., 12 × 10= 120) on one detection card 110 and analytes to be detected (i.e., 10 × 10= 100) on 10 detection strips on the other detection card 110 can be detected, i.e., 220 analytes to be detected.

Although the embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the scope of knowledge possessed by those skilled in the art. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

Claims (10)

1. Detection card, its characterized in that includes:

the detection test strip comprises a sample pad, a combination pad, a reaction pad and a water absorption pad which are sequentially connected, wherein 1-12 detection lines are arranged on the reaction pad;

the device comprises a shell, wherein a sample adding window and a visual window are arranged on the surface of the shell, 2-12 clamping grooves are arranged in the shell, and the distance between every two adjacent clamping grooves is 1-3 mm; the housing is configured such that when the test strip is fixed in the card slot, the sample application window corresponds to the sample pad, and the visible window corresponds to the reaction pad.

2. The test card of claim 1, wherein the sample pad is disposed on the conjugate pad.

3. The detection card of claim 1, wherein the combination pad, the reaction pad and the absorbent pad on the detection strip are sequentially overlapped with each other, and specifically comprise: one end of the combination pad, which is close to the reaction pad, is lapped at one end of the reaction pad, and one end of the water absorption pad, which is close to the reaction pad, is lapped at the other end of the reaction pad.

4. The test card of claim 3, wherein the length of the bonding pad overlapping the reaction pad is 1-2 mm.

5. The test card of claim 3, wherein the absorbent pad overlaps the reaction pad by a length of 1 to 2mm.

6. A colloidal gold-chemiluminescence detector applied to a detection card according to any one of claims 1 to 5, comprising:

an object stage for fixing the detection card;

the light-emitting capturing element is internally provided with a charge-coupled device, the charge-coupled device is used for capturing a colloidal gold signal and a chemiluminescence signal on the detection card, and the charge-coupled device is vertical to the objective table;

wherein the stage and the charge coupled device are configured to be capable of relative movement to vary a signal capture range of the charge coupled device to the test card.

7. The meter of claim 6, wherein the luminescence capture element is selected from any one of a camera or a video camera.

8. The detector of claim 6, wherein the luminescence capture element is provided with a light source, and when the light source is in an on state, the CCD is configured to capture the gold signal; the charge coupled device is used for capturing the chemiluminescence signal when the light source is in an off state.

9. The meter of claim 8, wherein the luminescence capture element is configured with a lens, the light source being disposed on the lens.

10. The apparatus of claim 6, wherein the CCD is vertically disposed above the stage.

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