CN217556208U

CN217556208U - Human bone marrow mesenchymal stem cell culture device applying three-dimensional cells

Info

Publication number: CN217556208U
Application number: CN202220070287.5U
Authority: CN
Inventors: 陈琳洁; 高岩嵩; 王琳
Original assignee: Jiuzhitang Meike Beijing Cell Technology Co ltd
Current assignee: Jiuzhitang Meike Beijing Cell Technology Co ltd
Priority date: 2022-01-12
Filing date: 2022-01-12
Publication date: 2022-10-11
Anticipated expiration: 2032-01-12

Abstract

The utility model discloses a human bone marrow mesenchymal stem cell culture device applying three-dimensional cells, which comprises a culture box body, wherein the middle part of the top end of the culture box body is hermetically connected with a box cover, the top end of the culture box body is fixedly connected with a v 21274shaped frame, two sides of the v 21274shaped frame are respectively provided with a sliding groove, the two sliding grooves are respectively connected with the box cover in a sliding way through a moving block, a linkage frame is arranged inside the culture box body, two culture dish support plates are fixedly arranged inside the linkage frame, the top ends of the two culture dish support plates are connected with a stem cell culture vessel in a laminating way, a nutrient storage barrel is fixedly arranged inside the box cover, and electromagnetic control valves are respectively arranged at three openings of the nutrient storage barrel.

Description

Human bone marrow mesenchymal stem cell culture device applying three-dimensional cells

Technical Field

The utility model relates to a culture apparatus, in particular to human bone marrow mesenchymal stem cell culture apparatus using three-dimensional cells, which belongs to the technical field of stem cell culture.

Background

Mesenchymal stem cells are pluripotent stem cells that have all of the commonality of stem cells, namely, self-renewal and multipotent differentiation capacity. The application is most in clinical application, and the combined application with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstitution. After a patient receives a large dose of chemotherapy, the mesenchymal stem cells and the hematopoietic stem cells are input together, so that the recovery time of the blood cells of the patient can be obviously accelerated, the safety is realized, no adverse reaction is caused, and the mesenchymal stem cells exist in bone marrow, skeletal muscle, periosteum and trabecula. Because of its wide range of differentiated tissue types, it is of great clinical value.

The stem cell culture needs to use a culture device, however, the existing culture device has some defects, for example, the existing culture device is inconvenient to take a culture dish inside the device, the culture dish cannot be quickly taken out when a culture box cover is opened, and in the process of culturing stem cells, a culture solution can be dripped by opening the box cover, and the culture solution is dripped one by one, which consumes time, therefore, the human bone marrow mesenchymal stem cell culture device using three-dimensional cells is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an use human bone marrow mesenchymal stem cells culture apparatus of three-dimensional cell to solve the problem that provides in the above-mentioned background art.

In order to achieve the above purpose, the utility model provides a following technical scheme: a human bone marrow mesenchymal stem cell culture device applying three-dimensional cells comprises a culture box body, wherein the middle part of the top end of the culture box body is hermetically connected with a box body cover, the top end of the culture box body is fixedly connected with a v-21274-shaped frame, two sides of the v-21274-shaped frame are respectively provided with a sliding groove, the two sliding grooves are respectively connected with the box body cover in a sliding manner through a moving block, a linkage frame is arranged in the culture box body, two culture dish support plates are fixedly arranged in the linkage frame, the top ends of the two culture dish support plates are connected with a stem cell culture vessel in an attaching manner, a nutrient storage barrel is fixedly arranged in the box body cover, electromagnetic control valves are respectively arranged at three openings of the nutrient storage barrel, the output ends of the three electromagnetic control valves are respectively connected with a short dropping pipeline and a long dropping pipeline, and the three short dropping pipelines and the three long dropping pipelines are vertically corresponding to the corresponding stem cell culture vessel.

As a preferred technical scheme of the utility model, the lateral side of the top end of the box body cover is fixedly provided with a vertical handle, one end of the vertical handle is fixedly connected with a buckle sleeve, the middle part of the front side of the v-shaped frame is fixedly provided with a telescopic connecting column, and one end of the telescopic connecting column is connected with the buckle sleeve in an inserting way.

As an optimal technical scheme of the utility model, the positive fixed mounting who cultivates the box has observation glass window, the surface rotation of observation glass window is connected with the shielding plate.

As an optimized technical proposal of the utility model, the inner wall of the two sides of the culture box body is fixedly connected with a heat isolating layer.

As an optimal technical scheme of the utility model, the back fixedly connected with fluid infusion hose of box lid, the one end and the nutrient solution storage cylinder intercommunication of fluid infusion hose.

As an optimal technical scheme of the utility model, one side fixed mounting of cultivateing the box has three electromagnetic switch, and is three solenoid electric valve is through the electromagnetic switch and the external power supply electric connection that correspond.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model relates to an use human bone marrow mesenchymal stem cells culture apparatus of three-dimensional cell, through the shape frame, sliding tray, linkage frame, vertical handle, buckle cover and the flexible spliced pole that are equipped with, open the box lid through vertical handle, utilize the linkage frame can be quick take out stem cells culture household utensils, it is convenient to take, save time.

2. The utility model relates to an use human bone marrow mesenchymal stem cells culture apparatus of three-dimensional cell, through nutrient solution storage cylinder, solenoid electric valve, short dropwise add pipeline and the long dropwise add pipeline that is equipped with, at culture apparatus in-process of cultivateing stem cell, make nutrient solution dropwise add to corresponding stem cell culture household utensils through the switching of control solenoid electric valve, need not open box lid alright dropwise add culture solution, also need not dropwise add one by one, save the time of cultivateing, the practicality is strong.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the internal structure of the incubator body of the present invention;

FIG. 3 is a schematic view of the structure of the incubator body of the present invention.

In the figure: 1. a culture box body; 2. a case cover; 3. \ 21274; 4. a vertical handle; 5. a buckle sleeve; 6. a telescopic connecting column; 7. a sliding groove; 8. an observation glass window; 9. a shielding plate; 10. a culture dish support plate; 11. a stem cell culture vessel; 12. a nutrient storage cylinder; 13. an electromagnetic control valve; 14. a short dropping pipe; 15. a long dropping pipeline; 16. a linking frame; 17. a thermal insulating layer; 18. an electromagnetic switch; 19. fluid infusion hose.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution of a human bone marrow mesenchymal stem cell culture apparatus using three-dimensional cells:

according to the graph shown in fig. 1-3, the culture box comprises a culture box body 1, wherein a box body cover 2 is hermetically connected to the middle of the top end of the culture box body 1, a v-212744-shaped frame 3 is fixedly connected to the top end of the culture box body 1, sliding grooves 7 are formed in two sides of the v-212743, the two sliding grooves 7 are slidably connected with the box body cover 2 through moving blocks, a linkage frame 16 is installed inside the culture box body 1, two culture dish support plates 10 are fixedly installed inside the linkage frame 16, the top ends of the two culture dish support plates 10 are connected with a dry cell culture dish 11 in an attaching mode, a nutrient storage barrel 12 is fixedly installed inside the box body cover 2, electromagnetic control valves 13 are installed at three openings of the nutrient storage barrel 12, the output ends of the three electromagnetic control valves 13 are connected with a short dropping pipeline 14 and a long dropping pipeline 15, and the three short dropping pipelines 14 and the three long dropping pipelines 15 correspond to the corresponding dry cell culture dishes 11 in an up-and-down mode.

According to the technical scheme, as shown in fig. 1 and fig. 2, a vertical handle 4 is fixedly mounted on the side of the top end of a box body cover 2, one end of the vertical handle 4 is fixedly connected with a buckle sleeve 5 and 21274, a telescopic connecting column 6 is fixedly mounted in the middle of the front of a shape frame 3, one end of the telescopic connecting column 6 is connected with the buckle sleeve 5 in an inserting mode, the box body cover 2 is conveniently fixed to a specified height, an observation glass window 8 is fixedly mounted on the front of the cultivation box body 1, a shielding plate 9 is rotatably connected to the surface of the observation glass window 8, cultivation conditions can be conveniently observed, good light illumination isolation effects can be conveniently achieved, heat isolation layers 17 are fixedly connected to the inner walls of the two sides of the cultivation box body 1, external heat is isolated, a liquid supplementing hose 19 is fixedly connected to the back of the box body cover 2, one end of the liquid supplementing hose 19 is communicated with a nutrient storage cylinder 12, nutrient solution can be conveniently supplemented, three electromagnetic switches 18 are fixedly mounted on one side of the cultivation box body 1, and three electromagnetic valves 13 are electrically connected with an external power supply through the corresponding electromagnetic switches 18.

When specifically using, the utility model relates to an use human bone marrow mesenchymal stem cell culture apparatus of three-dimensional cell, the inside of stem cell culture household utensils 11 is placed to the stem cell that processing needs to be cultivateed, open box lid 2 through vertical handle 4, utilize linkage frame 16 can be quick take out culture dish support plate 10, place culture dish support plate 10 department with stem cell culture household utensils 11 one by one again, it can to go up box lid 2 and cultivate, at the in-process of cultivateing, make the nutrient solution dropwise add to the stem cell culture household utensils 11 that corresponds through the switching of control solenoid electric valve 13, need not open box lid 2 alright dropwise add culture solution, also need not individual dropwise add, save the time of cultivateing, therefore, the clothes hanger is strong in practicability, after the cultivation, again, open box lid 2 through vertical handle 4, utilize linkage frame 16 can be quick take out stem cell culture household utensils 11, directly take out can, it is convenient to take, save time.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The human bone marrow mesenchymal stem cell culture device applying the three-dimensional cells comprises a culture box body (1), and is characterized in that the middle of the top end of the culture box body (1) is hermetically connected with a box body cover (2), the top end of the culture box body (1) is fixedly connected with a v-21274-shaped frame (3), two sides of the v-212-shaped frame (3) are respectively provided with a sliding groove (7), two sliding grooves (7) are respectively connected with the box body cover (2) in a sliding manner through a moving block, a linkage frame (16) is arranged inside the culture box body (1), two culture dish support plates (10) are fixedly arranged inside the linkage frame (16), the top ends of the two culture dish support plates (10) are connected with a stem cell culture vessel (11) in an attaching manner, a nutrient storage cylinder (12) is fixedly arranged inside the box body cover (2), electromagnetic control valves (13) are respectively arranged at three openings of the nutrient storage cylinder (12), the output ends of the three electromagnetic control valves (13) are respectively connected with a short dripping pipeline (14) and a long dripping pipeline (15), and the electromagnetic control valves (14) correspond to the stem cell culture vessel (11).

2. The human bone marrow mesenchymal stem cell culture device applying the three-dimensional cells according to claim 1, wherein: a vertical handle (4) is fixedly mounted on the side of the top end of the box body cover (2), a buckle sleeve (5) is fixedly connected to one end of the vertical handle (4), a telescopic connecting column (6) is fixedly mounted in the middle of the front face of the rectangular frame (3), and one end of the telescopic connecting column (6) is connected with the buckle sleeve (5) in an inserting mode.

3. The human bone marrow mesenchymal stem cell culture device applying the three-dimensional cells according to claim 1, wherein: the front surface of the culture box body (1) is fixedly provided with an observation glass window (8), and the surface of the observation glass window (8) is rotatably connected with a baffle plate (9).

4. The human bone marrow mesenchymal stem cell culture apparatus using three-dimensional cells according to claim 1, wherein: the inner walls of the two sides of the incubator body (1) are fixedly connected with heat insulation layers (17).

5. The human bone marrow mesenchymal stem cell culture device applying the three-dimensional cells according to claim 1, wherein: the back of the box body cover (2) is fixedly connected with a liquid supplementing hose (19), and one end of the liquid supplementing hose (19) is communicated with the nutrient storage cylinder (12).

6. The human bone marrow mesenchymal stem cell culture apparatus using three-dimensional cells according to claim 1, wherein: three electromagnetic switches (18) are fixedly mounted on one side of the culture box body (1), and the three electromagnetic control valves (13) are electrically connected with an external power supply through the corresponding electromagnetic switches (18).