CN217521023U - Portable fluorescent real-time detection device for detecting drugs in fingerprint sweat - Google Patents
Portable fluorescent real-time detection device for detecting drugs in fingerprint sweat Download PDFInfo
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- CN217521023U CN217521023U CN202122991021.2U CN202122991021U CN217521023U CN 217521023 U CN217521023 U CN 217521023U CN 202122991021 U CN202122991021 U CN 202122991021U CN 217521023 U CN217521023 U CN 217521023U
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Abstract
The utility model discloses a portable fluorescence real-time detection device that is arranged in fingerprint sweat drugs to detect, include: the box body is internally provided with a sample collection chamber and a detection observation chamber, and is also provided with a sample adding hole, an observation window and a test paper taking and placing opening, wherein the sample adding hole and the test paper taking and placing opening correspond to the sample collection chamber, and the observation window corresponds to the detection observation chamber; the test paper strip, the test paper strip can be fixed in the box, the ultraviolet light source sets up in detect the observation room to at least be used for to the test paper strip detect the part shine. The utility model provides a pair of a portable fluorescence real-time detection device for drug detection in fingerprint sweat, simple structure, low cost, carry easily, make things convenient for the sampling, easily carry out fingerprint sweat drug detection on the spot.
Description
Technical Field
The utility model relates to a drugs detection device, in particular to a portable fluorescence real-time detection device that is arranged in fingerprint sweat drugs to detect belongs to drugs detection technical field.
Background
Drugs refer to narcotics and psychotropic drugs that can cause addiction. How to more effectively attack drug crimes, grasp the evidence of drug selling and drug absorbing, and develop more effective drug inspection and detection methods to meet the actual pursuit and control requirements of continuous complexity and continuous increase of quantity has important practical significance. Currently, urine, saliva, hair and blood are mainly used for on-site rapid detection of drugs, but the acquisition of the detection materials such as urine is limited by the matching of a subject, saliva is easy to be polluted to influence the detection result, hair can be detected after the drug is inhaled for at least one month, and the detection of blood needs complex sample pretreatment and professional instruments. On the occasions where people flow is highly concentrated and rapid screening is needed, the materials and the traditional detection method cannot meet the requirements of rapid strike, management and control of drug trafficking and drug absorption.
SUMMERY OF THE UTILITY MODEL
The main object of the present invention is to provide a portable fluorescence real-time detection device for detecting drugs in fingerprint sweat, so as to overcome the disadvantages of the prior art.
For realizing the purpose of the utility model, the utility model discloses a technical scheme include:
the embodiment of the utility model provides a pair of portable fluorescence real-time detection device that is arranged in fingerprint sweat drugs to detect, include:
the box body is internally provided with a sample collection chamber and a detection observation chamber, the box body is also provided with a sample adding hole, an observation window and a test paper taking and placing opening, the sample adding hole and the test paper taking and placing opening correspond to the sample collection chamber and are communicated with the sample collection chamber, the test paper taking and placing opening is also provided with a box door which can be opened and closed, and the observation window corresponds to the detection observation chamber;
the test strip is at least used for detecting drugs and metabolites thereof in human sweat by using a drug detection card, the test strip can be fixed in the box body, when the test strip is arranged in the box body, a sampling part of the test strip is correspondingly arranged in the sample collection chamber and corresponds to the sample adding hole, and a detection part of the test strip is correspondingly arranged in the sample collection chamber;
and the ultraviolet light source is arranged in the detection observation chamber and is at least used for irradiating the detection part of the test strip.
Compared with the prior art, the utility model has the advantages that:
1) the portable fluorescent real-time detection device for detecting drugs in fingerprint sweat provided by the embodiment of the utility model has the advantages of simple structure, low cost, easy carrying, convenient sampling and easy on-site fingerprint sweat drug detection;
2) the embodiment of the utility model provides a portable fluorescence real-time detection device for drug detection in fingerprint sweat, which is convenient to carry, simple to sample, simple and convenient to operate and standard in the testing process, and suitable for field detection of various drugs such as public security, sanitation and the like;
3) the portable fluorescent real-time detection device for detecting drugs in fingerprint sweat provided in the embodiment of the utility model has low requirement on detection environment, short detection period and high efficiency, and is suitable for portable detection;
4) the embodiment of the utility model provides a pair of portable fluorescence real-time detection device for drug detection in fingerprint sweat is convenient for to the real time monitoring of sample, is suitable for witnessed inspections more.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic structural diagram of a portable fluorescence real-time detection device for detecting drugs in fingerprint sweat according to an exemplary embodiment of the present invention;
fig. 2 is a schematic structural diagram of a test strip provided in an exemplary embodiment of the present invention;
fig. 3 is a schematic side view of a test strip provided in an exemplary embodiment of the present invention;
fig. 4 is a schematic structural diagram of a test strip accommodating mechanism provided in an exemplary embodiment of the present invention;
fig. 5 is a schematic structural diagram of a sample loading liquid accommodating mechanism according to an exemplary embodiment of the present invention.
Detailed Description
In view of the deficiencies in the prior art, the inventor of the present invention has made extensive studies and practices to provide the technical solution of the present invention. The technical solution, its implementation and principles, etc. will be further explained as follows.
The embodiment of the utility model provides a pair of portable fluorescence real-time detection device that is arranged in fingerprint sweat drug to detect, include:
the box body is internally provided with a sample collection chamber and a detection observation chamber, and is also provided with a sampling hole, an observation window and a test paper taking and placing opening, wherein the sampling hole and the test paper taking and placing opening correspond to and are communicated with the sample collection chamber, the test paper taking and placing opening is also provided with a box door which can be opened and closed, and the observation window corresponds to the detection observation chamber;
the test strip is at least used for detecting drugs and metabolites thereof in human sweat by using a drug detection card, the test strip can be fixed in the box body, when the test strip is arranged in the box body, a sampling part of the test strip is correspondingly arranged in the sample collection chamber and corresponds to the sample adding hole, and a detection part of the test strip is correspondingly arranged in the sample collection chamber;
and the ultraviolet light source is arranged in the detection observation chamber and is at least used for irradiating the detection part of the test strip.
In some more specific embodiments, at least the inner wall of the sample collection chamber is provided with a fixing groove matched with the test strip, and the width of the fixing groove is larger than that of the test strip.
In some more specific embodiments, the fixed slot extends from the sample collection chamber to the detection viewing chamber, and the length of the fixed slot is less than the length of the test strip.
In some more specific embodiments, the inner wall of the sample collection chamber is further provided with a sample collection area matched with the finger pulp of a human finger, the sample collection area is provided with a part overlapped with at least the sample collection area and the fixing groove, and the bottom of the sample collection area is a hollow structure.
In some specific embodiments, a partition is disposed in the housing, the partition separates a space inside the housing into the sample collection chamber and the detection observation chamber, and a gap through which the test strip can pass is formed between the partition and the bottom of the fixing groove.
In some specific embodiments, a sample conveying pipe is further disposed in the sample collection chamber, one end of the sample conveying pipe is connected to the sample addition hole, and the other end of the sample conveying pipe faces the sample collection area.
In some specific embodiments, a power supply is disposed outside the box body, and the power supply is electrically connected to the ultraviolet light source.
In some more specific embodiments, the test strip comprises a base plate, and a sample pad, a labeling pad, a detection membrane and an absorption pad disposed on the base plate, wherein the sample pad, the labeling pad, the detection membrane and the absorption pad are connected or contacted in sequence,
the detection membrane is provided with a detection line containing a drug coating antigen and a quality control line containing goat-anti-mouse secondary antibodies, and the sample loading bottle is filled with sample loading liquid.
In some specific embodiments, the bottom plate comprises a PVC plate, the sample pad, the labeling pad, the detection membrane and the absorption pad are adhered to the PVC plate, the surface of the sample pad is further provided with a fingerprint collection layer for collecting fingerprints, and the detection membrane comprises a nitrocellulose membrane.
In some more specific embodiments, the portable fluorescence real-time detection device further comprises: the test paper accommodating mechanism is used for accommodating the test paper strips.
In some specific embodiments, a test paper accommodating chamber is further disposed in the box, the test paper accommodating chamber is isolated from the sample collecting chamber and the detection observing chamber, and the test paper accommodating mechanism is movably disposed in the test paper accommodating chamber, or the test paper accommodating mechanism and the test paper accommodating chamber are integrally disposed.
In some more specific embodiments, the portable fluorescence real-time detection device further comprises: and the sample liquid storage mechanism is used for accommodating sample liquid.
In some specific embodiments, a sample liquid accommodating chamber is further disposed in the box, the sample liquid accommodating chamber is isolated from the sample collecting chamber and the detection observing chamber, and the sample liquid accommodating mechanism is movably disposed in the sample liquid accommodating chamber, or the sample liquid accommodating mechanism and the sample liquid accommodating chamber are integrally disposed.
In the following, the technical solution, the implementation process and the principle thereof will be further explained with reference to the drawings, unless otherwise specified, the sample pad, the label pad, the detection membrane, the absorption pad, the sample solution and other reagents or drugs, and the ultraviolet light source, etc. used in the embodiments of the present invention may be known to those skilled in the art, and the specific structure and material thereof are not limited herein, and they are all commercially available.
Referring to fig. 1-3, a portable fluorescent real-time detection device for detecting drugs in fingerprint sweat comprises a case 100 for realizing sample collection and result reading, an ultraviolet light source 9, a test strip 300, a test strip accommodating mechanism 400 and a sample loading liquid storage mechanism 500, wherein the case 100 is internally provided with a sample collection chamber 110 and a detection observation chamber 120 which are isolated from each other, the case 100 is further provided with a sample adding hole 12, an observation window 11 and a test strip taking and placing opening, the sample adding hole 12 and the test strip taking and placing opening correspond to and are communicated with the sample collection chamber 110, the test strip taking and placing opening is further provided with a case door 200 which can be opened and closed, and the observation window 11 corresponds to the detection observation chamber 120;
the test strip 300 is at least used for detecting drugs and metabolites thereof in human sweat by using a drug detection card, the test strip 300 can be fixed in the case 100, and when the test strip 300 is disposed in the case 100, a sampling portion of the test strip 100 is correspondingly disposed in the sample collection chamber 110 and corresponds to the sample application hole 12, and a detection portion of the test strip 300 is correspondingly disposed in the sample collection chamber 120 and corresponds to the observation window 11;
the ultraviolet light source 9 is disposed in the detection observation chamber 120 and at least used for irradiating a detection portion of the test strip 300, the test strip accommodating mechanism 400 is used for accommodating the test strip 300, and the sample loading solution storage mechanism 500 is used for accommodating a sample loading solution.
In this embodiment, at least the inner wall of the sample collection chamber 110 is provided with a fixing groove 6 matched with the test strip 300, the width of the fixing groove 6 is greater than the width of the test strip 300, or the fixing groove 6 further extends from the sample collection chamber 110 to the detection observation chamber 120, and the length of the fixing groove 6 is smaller than the length of the test strip 300, for example, the width of the fixing groove is 0.5mm wider than the test strip 300, the length is 2mm shorter than the test strip 300, and the depth of the fixing groove is 1.5mm, so that the test strip can be inserted into the fixing groove, and can be conveniently taken out of the fixing groove after the test of the test strip is completed.
In this embodiment, the inner wall of the sample collection chamber 110 is further provided with a sample collection area 7 matching with the finger web of a human finger, at least a part of the sample collection area 7 overlaps with the fixing groove 6, wherein the bottom of the sample collection area 7 is a hollow structure, the hollow part of the sample collection area 7 is a sample pad area exposed by a test strip, the finger web of the finger presses the sample pad area to adsorb the sample, and further, the hollow part of the test strip line display area is a fluorescent test strip detection film, so that C, T line display is displayed.
In this embodiment, the sample collection area 7 is oval, the apex of the major axis of the sample collection area 7 is 2mm from the edge of the housing, and the edge of the sample collection area 7 is chamfered at 45 °, although the angle can be fine-tuned based thereon.
In this embodiment, the sample collection area 7 may be disposed at the bottom of the sample collection chamber 110, the well 12 is disposed at the top of the sample collection chamber 110, and the sample collection area 7 is located in the axial direction of the well 12.
In this embodiment, a sample delivery tube is further disposed in the sample collection chamber 110, and one end of the sample delivery tube is connected to the sample application hole 12, and the other end of the sample delivery tube faces the sample collection area 7, for example, the length of the sample delivery tube is 10mm, so as to allow the sample solution to drip vertically downwards.
In this embodiment, the test strip line-displaying area 8 is disposed in the detection observation chamber 120, and at least a portion of the test strip line-displaying area 8 overlaps the fixing groove 6, in this embodiment, the test strip line-displaying area 8 may be disposed at the bottom of the detection observation chamber 120, the observation window 11 is correspondingly disposed at the top of the detection observation chamber 120, and the observation window 11 is located right above the test strip line-displaying area for observing C, T line-displaying condition.
In this embodiment, the test strip line displaying area 8 is a hollow rectangle having the same width as the test strip, and the edge of the test strip line displaying area 8 has a 45 ° chamfer, which may be fine-tuned on the basis, wherein the test strip line displaying area and the sample collecting area are on the same horizontal plane.
In this embodiment, the test strip fixing groove, the sample collection area, the test strip display area and the long symmetry axis of the bottom surface of the box body are on the same straight line, and the circle center of the sample adding hole is on the long symmetry axis of the top surface of the box body.
In this embodiment, a partition is disposed in the case 100, the partition separates the space inside the case 100 into the sample collection chamber 110 and the detection observation chamber 120, and a gap through which the test strip 600 can pass is formed between the partition and the bottom of the fixed slot 6.
In this embodiment, the box 100 is a rectangular parallelepiped sealed box made of opaque black rubber, the thickness of the box wall and the middle partition of the box 100 is 1.5mm, and the observation window 11 is a transparent glass window.
In this embodiment, a power supply 10 is disposed outside the box 100, the power supply 10 is electrically connected to the ultraviolet light source 9, and the power supply 10 may be a mobile power supply, such as a button battery; the ultraviolet light source 9 includes an ultraviolet lamp or the like, and the wavelength of ultraviolet light supplied from the ultraviolet lamp may be 365 nm.
In this embodiment, a switch is further disposed outside the box 100, and the switch is electrically connected to the power supply 10 and the ultraviolet light source 9, for example, the switch may be a boat-shaped switch; of course, components such as a circuit board for controlling the on/off of the ultraviolet light source 9 may also be included, which are not specifically described herein.
In this embodiment, the test strip 300 comprises a base plate 1, and a sample pad 2, a label pad 3, a detection membrane 4 and an absorption pad 5 disposed on the base plate 1, wherein the sample pad 2, the label pad 3, the detection membrane 4 and the absorption pad 5 are connected or contacted in sequence,
wherein, the sample pad 2 is further provided with a pressing sampling area (not shown in the figure) matched with the finger belly of a human finger, the label pad 3 contains a conjugate of the time-resolved fluorescent microsphere and the drug antibody, and the detection membrane 4 is further provided with a test line T (hereinafter, may be simply referred to as T line) containing the drug coating antigen and a quality control line C (hereinafter, may be simply referred to as C line) containing the goat-anti-mouse secondary antibody.
In this embodiment, bottom plate 1 includes the PVC board, sample pad 2, mark pad 3, detection membrane 4 and absorption pad 5 paste on the PVC board, the surface of sample pad 2 still is provided with the fingerprint collection layer that is used for gathering the fingerprint, detection membrane 4 includes nitrocellulose membrane.
In this embodiment, the test strip sample pad material is easy to absorb fingerprint sweat, the test strip has a wide size, the sample pad has a large area of fingerprint collection area, and the marker pad bears the compound of the time-resolved fluorescent microsphere labeled antibody.
In this embodiment, during the forward migration of the sample on the sample pad 2, the target is firstly combined with the conjugate on the label pad 2 by the antigen-antibody specific binding, and cannot be combined with the coating on the T-line because the antibody sites on the conjugate are occupied; during the forward migration, the secondary antibody on the C line is combined with the conjugate antibody, and then the time-resolved fluorescent microspheres are captured to display the line.
In this embodiment, a test paper accommodating chamber is further disposed in the box 100, the test paper accommodating chamber is isolated from the sample collecting chamber and the detection observing chamber, the test paper accommodating mechanism 400 is movably disposed in the test paper accommodating chamber, or the test paper accommodating mechanism 400 and the test paper accommodating chamber are integrally disposed.
In this embodiment, referring to fig. 4, the test paper accommodating mechanism 400 may be a test paper storage bottle, and the overall height of the test paper storage bottle is slightly greater than the length of the fluorescent test paper, so that the test paper can be stored conveniently, and the bottle body and the bottle cap are not separated; for example, the test strip storage bottle is cylindrical and made of plastic, the bottle cap is not separated from the bottle body, the bottle body is formed by hollow blow molding, the bottle cap is formed by injection molding, a circle of groove is formed in the bottle mouth, and the bottle body is closed by meshing with a circle of protrusion of the bottle cap; of course, the test strip storage bottle can be obtained commercially.
In this embodiment, a sample liquid accommodating chamber is further disposed in the box, the sample liquid accommodating chamber is isolated from the sample collecting chamber and the detection observing chamber, and the sample liquid accommodating mechanism 500 is movably disposed in the sample liquid accommodating chamber, or the sample liquid accommodating mechanism 500 and the sample liquid accommodating chamber are integrally disposed.
In this embodiment, referring to fig. 5, the sample solution accommodating mechanism 500 may be a sample solution storage bottle, the sample solution storage bottle includes a bottle body, a bottle cap and a dropper cap, and the volume of the sample solution storage bottle is 15 mL; when going up appearance liquid storage bottle application of sample, insert the burette and add the appearance hole, drip through the extrusion body and add appearance liquid, wherein the material of body is the soft plastics material, and the bottle lid is the plastics material, passes through threaded connection with the body, and bottle lid upper portion central point puts and is provided with the burette that is 2cm long, and the burette external diameter is 3mm, and the internal diameter is 2mm, the burette cap passes through soft plastics and links to each other with the bottle lid, of course, this goes up appearance liquid storage bottle and can purchase the acquisition in market.
In this embodiment, the sample liquid storage bottle is made of a soft plastic material, so that the sample liquid is conveniently extruded and dripped; the outer diameter of the dropper protruding from the bottle cap is slightly smaller than the diameter of the sample adding hole, so that the dropper can be inserted into the sample adding hole conveniently to accurately drip the sample adding liquid onto the sample pad.
In this embodiment, the preparation method of the fluorescent test strip in the portable fluorescent real-time detection device for detecting drugs in fingerprint sweat may include the following steps:
(1) sample pad treatment: cutting the glass cellulose membrane into strips with the width of 300 x 18mm, placing the strips into the sample pad treatment solution for soaking for 2 hours, taking out the strips, and drying the strips in a 37 ℃ drying oven to obtain the required sample pad; wherein the sample pad treatment solution comprises a buffer system containing 0.3 wt% triton x-100, 0.2mM NaCl, 1 wt% sucrose and 0.05M Tris-HCl, and has a pH of 8.0;
(2) pretreatment of the marking pad: cutting the glass fiber film into strips with the width of 300 x 6mm, putting the strips into the marking pad treatment solution, soaking for 2 hours, drying in a drying oven at 37 ℃, and then cutting the strips into sheets with the width of 6 x 6mm on a strip cutting machine to obtain the required marking pads; wherein the marking pad treatment solution is a buffer system containing 5 wt% of sucrose, 2 wt% of trehalose, 1 wt% of polyethylene glycol, 0.5 wt% of tween 20 and 0.05M boric acid buffer solution, and the pH value is 8.2;
(3) preparing the absorbent pad: cutting the absorbent paper into strips with the size of 300 x 18mm to obtain the required absorbent pad;
(4) treatment of nitrocellulose membrane: respectively spraying a secondary antibody serving as a quality control line marker and a drug coating antigen serving as a test line marker at the middle position of the nitrocellulose membrane, and drying to finish the treatment of the nitrocellulose membrane;
(5) assembling the fluorescent test strip: and sequentially sticking a sample pad, a marking pad, a nitrocellulose membrane and a water absorption pad on a bottom plate with the thickness of 300 x 60mm along the transverse direction, and ensuring that adjacent stickers are partially overlapped to obtain the drug fluorescence detection test strip.
In this embodiment, the method for dropping the colloidal gold and antibody conjugate on the labeling pad may include:
(1) adding 50 mu L of time-resolved fluorescent microspheres diluted by 100 times into 400 mu L of 0.05M boric acid buffer solution, uniformly mixing, adding 10-30 mu L of EDC and NHS with the concentrations of 10mg/mL, and activating for 1h at room temperature;
(2) adding 2-6 mu L of 1mg/mL drug monoclonal antibody into the activated system, oscillating and incubating for 1h at normal temperature, adding 50-150 mu L of 10 wt% bovine serum albumin, and oscillating and sealing for 1h at normal temperature;
(3) and centrifuging the closed system at 13000rpm for 15min, discarding the supernatant, redissolving by using 10 wt% of bovine serum albumin to obtain a final compound, dripping the conjugate into a prepared 3 x 6mm mark pad in an amount of 4.5 mu L/pad, drying, and assembling the drug fingerprint sweat fluorescent test strip.
The embodiment of the utility model provides a portable fluorescence real-time detection device's that is arranged in fingerprint sweat to detect theory of operation includes:
the device mainly aims at the fact that the drug to be detected is a micromolecular chemical substance, so the working detection principle of the device is a competition method, namely the drug molecules in a sample and the conjugate of the antibody and the fluorescent microspheres loaded on the coated original competition marking pad fixed on the nitrocellulose membrane.
Specifically, when a sample to be tested is directly tested, the sample is finally processed into a liquid state and is directly dripped onto the sample pad through the sample adding hole for testing;
when a suspected toxin-absorbing person is detected, firstly, the test strip is taken out of the test strip accommodating mechanism, a box door of a sample collecting chamber is opened, the test strip is inserted into a test strip fixing groove in the box body forwards from one end of a water absorption pad, the finger abdomen of the person to be detected is pressed to a sampling area for sampling, a dropper cap of the sample loading liquid storage mechanism is opened, a dropper is inserted into a sample adding hole, and the sample loading liquid storage bottle is loosened after being slightly squeezed for about 1 second, so that sample loading is completed; and (4) turning on the ultraviolet lamp, and observing the line-developing condition of the test strip C, T at any time through an observation window of the detection observation chamber.
Specifically, the drug attached to the sample pad migrates forward after being dissolved in the sample loading solution, the sample solution migrates along the test strip from the direction of the sample pad to the direction of the water absorption pad through capillary action, in the process, the dried fluorescent microspheres on the marking pad and the antibody conjugate are dissolved and move forward continuously, if the tested sample does not contain the drug substance to be tested, the conjugate directly reacts with the drug coating antigen on the testing line for immunoreaction and is combined, so that the fluorescent microspheres are captured on the test line for aggregation and color development to generate a fluorescent line, the residual conjugate which is not combined with the coating antigen continues to move forwards, after secondary immunoreaction with goat-anti-mouse secondary antibody sprayed on the quality control line, the goat-anti-mouse secondary antibody is captured on the quality control line and gathered to form a fluorescent line, therefore, two lines appear on the nitrocellulose membrane, which shows that the detected sample is negative and does not contain drugs, and the corresponding detected person does not contain drugs in vivo;
if the tested sample contains drugs, the sample is transferred from the sample pad to the marking pad, the fluorescent microspheres and the antibody conjugates firstly have immunoreaction with the drug molecules in the sample, and when the unreacted conjugates remain, the unreacted conjugates and the drug coating antigens on the test line have immunoreaction to form a fluorescent line on the test line, and the color intensity of the fluorescent line is weaker than that of the test line in a negative state; when the conjugate is completely immunoreactive with the drug in the sample, the conjugate can not be combined with the coating antigen on the test line, so that the test line can not show the line; the quality control line is set for checking whether the testing result of the portable device is valid, so the quality control line should display whether the drug component exists in the sample, if the wireless strip of the quality control line appears, the testing result of the device is invalid.
The portable fluorescent real-time detection device for detecting drugs in fingerprint sweat provided by the embodiment of the utility model has the advantages of simple structure, low cost, easy carrying, convenient sampling and easy on-site fingerprint sweat drug detection; in addition, the portable fluorescent real-time detection device for detecting drugs in fingerprint sweat provided by the embodiment of the utility model has the advantages of convenient carrying, simple sampling, simple and standard operation of the test process, and suitability for the field detection of various drugs such as public security, sanitation and the like; the portable fluorescent real-time detection device for detecting drugs in fingerprint sweat provided by the embodiment of the utility model has the advantages of low requirement on detection environment, short detection period and high efficiency, and is suitable for portable detection; additionally, the embodiment of the utility model provides a portable fluorescence real-time detection device for drug detection in fingerprint sweat has still set up the ultraviolet lamp, is convenient for to the real time monitoring of sample, is suitable for the witnessed inspections more.
It should be understood that the above-mentioned embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, and therefore, the protection scope of the present invention should not be limited thereby. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (13)
1. A portable fluorescence real-time detection device for detecting drugs in fingerprint sweat is characterized by comprising:
the box body is internally provided with a sample collection chamber and a detection observation chamber, the box body is also provided with a sample adding hole, an observation window and a test paper taking and placing opening, the sample adding hole and the test paper taking and placing opening correspond to the sample collection chamber and are communicated with the sample collection chamber, the test paper taking and placing opening is also provided with a box door which can be opened and closed, and the observation window corresponds to the detection observation chamber;
the test strip is at least used for detecting drugs and metabolites thereof in human sweat by using a drug detection card, the test strip can be fixed in the box body, when the test strip is arranged in the box body, a sampling part of the test strip is correspondingly arranged in the sample collection chamber and corresponds to the sample adding hole, and a detection part of the test strip is correspondingly arranged in the sample collection chamber;
and the ultraviolet light source is arranged in the detection observation chamber and is at least used for irradiating the detection part of the test strip.
2. The portable fluorescent real-time detection device of claim 1, wherein: at least the inner wall of the sample collection chamber is provided with a fixing groove matched with the test strip, and the width of the fixing groove is larger than that of the test strip.
3. The portable fluorescent real-time detection device of claim 2, wherein: the fixed groove extends into the detection observation chamber from the sample collection chamber, and the length of the fixed groove is smaller than that of the test strip.
4. The portable fluorescent real-time detection device of claim 2, wherein: the inner wall of the sample collection chamber is also provided with a sample collection area matched with the finger belly of a human finger, the sample collection area is provided with a part which is overlapped with the fixing groove at least in the sample collection area, and the bottom of the sample collection area is of a hollow structure.
5. The portable fluorescent real-time detection device of claim 3, wherein: the box is internally provided with a partition board which divides the space inside the box into a sample collection chamber and a detection observation chamber, and a gap for the test paper to pass through is formed between the partition board and the bottom of the fixed groove.
6. The portable fluorescent real-time detection device of claim 5, wherein: still be provided with the sample conveyer pipe in the sample collection room, the one end of sample conveyer pipe with add the appearance jogged joint, the other end orientation the sample collection district.
7. The portable fluorescent real-time detection device of claim 1, wherein: and a power supply is arranged outside the box body and electrically connected with the ultraviolet light source.
8. The portable fluorescent real-time detection device of claim 1, wherein: the test strip comprises a bottom plate, and a sample pad, a marking pad, a detection membrane and an absorption pad which are arranged on the bottom plate, wherein the sample pad, the marking pad, the detection membrane and the absorption pad are sequentially connected or contacted,
the detection membrane is provided with a detection membrane, a sample pad and a marking pad, wherein the sample pad is further provided with a pressing sampling area matched with the finger belly of a human finger, the marking pad is internally provided with a conjugate of a time-resolved fluorescent microsphere and a drug antibody, and the detection membrane is further provided with a test line containing a drug coating antigen and a quality control line containing a goat-anti-mouse secondary antibody.
9. The portable fluorescent real-time detection device of claim 8, wherein: the bottom plate comprises a PVC plate, the sample pad, the marking pad, the detection membrane and the absorption pad are pasted on the PVC plate, the surface of the sample pad is further provided with a fingerprint collection layer for collecting fingerprints, and the detection membrane comprises a nitrocellulose membrane.
10. The portable fluorescent real-time detection device of claim 1, further comprising: the test paper accommodating mechanism is used for accommodating the test paper strips.
11. The portable fluorescent real-time detection device of claim 10, wherein: the box body is also internally provided with a test paper accommodating chamber, the test paper accommodating chamber is mutually isolated from the sample collecting chamber and the detection observation chamber, and the test paper accommodating mechanism is movably arranged in the test paper accommodating chamber, or the test paper accommodating mechanism and the test paper accommodating chamber are integrally arranged.
12. The portable fluorescent real-time detection device of claim 1, further comprising: and the sample liquid storage mechanism is used for accommodating sample liquid.
13. The portable fluorescent real-time detection device of claim 12, wherein: the box is internally provided with a sample liquid accommodating chamber, the sample liquid accommodating chamber is mutually isolated from the sample collecting chamber and the detection observing chamber, and the sample liquid accommodating mechanism is movably arranged in the sample liquid accommodating chamber, or the sample liquid accommodating mechanism and the sample liquid accommodating chamber are integrally arranged.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122991021.2U CN217521023U (en) | 2021-12-01 | 2021-12-01 | Portable fluorescent real-time detection device for detecting drugs in fingerprint sweat |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122991021.2U CN217521023U (en) | 2021-12-01 | 2021-12-01 | Portable fluorescent real-time detection device for detecting drugs in fingerprint sweat |
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| CN217521023U true CN217521023U (en) | 2022-09-30 |
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| CN202122991021.2U Active CN217521023U (en) | 2021-12-01 | 2021-12-01 | Portable fluorescent real-time detection device for detecting drugs in fingerprint sweat |
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2021
- 2021-12-01 CN CN202122991021.2U patent/CN217521023U/en active Active
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