CN217398876U

CN217398876U - Nucleic acid amplification constant temperature detection device

Info

Publication number: CN217398876U
Application number: CN202221176173.5U
Authority: CN
Inventors: 夏小凯; 李佳伟; 焦天泽; 薛雅瑜
Original assignee: Shanghai Jienuo Shenghua Biotechnology Co ltd
Current assignee: Shanghai Jienuo Shenghua Biotechnology Co ltd
Priority date: 2022-05-10
Filing date: 2022-05-10
Publication date: 2022-09-09
Anticipated expiration: 2032-05-10

Abstract

The utility model discloses a nucleic acid amplification constant temperature detection device, which comprises a shell, wherein the shell is connected with a heat cover; the constant temperature unit is connected with the shell; the constant temperature unit comprises a heating assembly and a temperature sensor, wherein the heating assembly comprises a plurality of pipe holes for accommodating PCR test tubes; the detection unit is connected with the shell; the detection unit comprises a background light-emitting lamp, a color sensor and quartz glass; a control unit connected with the housing. The nucleic acid amplification constant-temperature detection device provided by the application supports real-time interpretation and countdown display, and can complete detection of a plurality of samples within 30 minutes. Small volume and portability. The temperature control precision is accurate. The built-in software/hardware double-layer over-temperature protection function ensures the stability of operation and the safety under unattended operation. The optical sensing is designed to be error proof and may sense whether an agent is placed in the device.

Description

Nucleic acid amplification constant temperature detection device

Technical Field

The utility model relates to a thermal-insulated bottom plate technical field especially relates to a portable nucleic acid amplification constant temperature detection device.

Background

The nucleic acid amplification technology is widely applied to the fields of medical diagnosis, animal and plant quarantine, food safety, transgenic detection and the like, has the advantages of high sensitivity, strong specificity and the like, and adopts the detection principle that a great amount of target genes are amplified by utilizing enzymatic reaction and then the detection of target objects is realized by measuring amplification products. Therefore, the simple and rapid quantitative determination of the nucleic acid amplification product is of great significance for developing a rapid detection method based on a nucleic acid amplification technology.

The isothermal nucleic acid amplification technology is to utilize the mixture of various enzymes, primers, deoxynucleotide triphosphate (dNTP), template DNA and buffer solution to perform a certain time of warm bath at the same temperature, so that different enzymes react with the DNA, thereby achieving the amplification of specific DNA fragments. Compared with the traditional PCR nucleic acid amplification, the isothermal nucleic acid amplification does not need switching between different temperatures, and the amplification of DNA fragments can be completed only by keeping the optimal temperature of enzyme reaction at 37 ℃, 42 ℃, 56 ℃ or 65 ℃. Compared with the temperature cycle of PCR nucleic acid amplification, the temperature control of the constant temperature nucleic acid amplification instrument is easier to realize. Therefore, the isothermal nucleic acid amplification-based instrument is simpler, cheaper and consumes less energy for heating.

In order to meet the requirement of batch detection of multiple samples, the nucleic acid amplification detector in the prior art is large in size. Meanwhile, most of PCR instruments in the market are used for detecting by using 96-well plates, the samples of the 96-well plates are in large demand, and users with small sample amount need to wait for the collection of the samples in order to collect more samples, so that the detection and the portable use can not be realized when the small sample amount comes.

SUMMERY OF THE UTILITY MODEL

The utility model provides a nucleic acid amplification constant temperature detection device.

The utility model provides a following scheme:

a nucleic acid amplification isothermal detection apparatus comprising:

a housing to which a thermal cover is connected;

the constant temperature unit is connected with the shell; the constant temperature unit comprises a heating assembly and a temperature sensor, wherein the heating assembly comprises a plurality of pipe holes for accommodating PCR test tubes;

the detection unit is connected with the shell; the detection unit comprises a background light-emitting lamp, a color sensor and quartz glass;

the control unit is connected with the shell, and the constant temperature unit and the detection unit are both in communication connection with the control unit;

the control unit is used for receiving the temperature value acquired by the temperature sensor and the color data value acquired by the color sensor, so that the heating assembly is controlled to provide constant temperature for the PCR test tube according to the temperature data value and an interpretation result is generated according to the color data value.

Preferably: the hot cover comprises a hot cover heating plate, the hot cover heating plate is provided with a groove, the groove is used for arranging a hot cover heating element, and the groove part removed by the hot cover heating plate is provided with a heat preservation material.

Preferably: the hot cover is hinged with the shell, so that the hot cover can be reversely opened relative to the shell, and the opening angle of the hot cover is not less than 90 degrees.

Preferably: the hinge joint of the hot cover and the shell is provided with a torsion spring, the hot cover is provided with a switch and a lock tongue, and the shell is provided with a lock hole matched with the lock tongue.

Preferably: the heating assembly includes a thin film heater chip and the temperature sensor includes a resistive temperature sensor.

Preferably: the front end face of the shell is provided with a display panel, and the display panel is in communication connection with the control unit.

Preferably: and a touch power switch is arranged on the front end face of the shell.

Preferably: and the rear end face of the shell is provided with a power socket.

Preferably: and a heat insulation bottom plate is arranged at the bottom of the shell.

Preferably: the PCR test tube is characterized by further comprising an optical sensor, the optical sensor is connected with the shell and is in communication connection with the control unit, and the control unit is further used for judging whether the PCR test tube is placed in the tube hole or not according to information acquired by the optical sensor.

According to the utility model provides a concrete embodiment, the utility model discloses a following technological effect:

through the utility model, a nucleic acid amplification constant temperature detection device can be realized, and in one realization mode, the device can comprise a shell, and the shell is connected with a heat cover; the constant temperature unit is connected with the shell; the constant temperature unit comprises a heating assembly and a temperature sensor, wherein the heating assembly comprises a plurality of pipe holes for accommodating PCR test tubes; the detection unit is connected with the shell; the detection unit comprises a background light-emitting lamp, a color sensor and quartz glass; the control unit is connected with the shell, and the constant temperature unit and the detection unit are both in communication connection with the control unit; the control unit is used for receiving the temperature value acquired by the temperature sensor and the color data value acquired by the color sensor, so that the heating assembly is controlled to provide constant temperature for the PCR test tube according to the temperature data value and an interpretation result is generated according to the color data value. The nucleic acid amplification constant-temperature detection device provided by the application supports real-time interpretation and countdown display, and can complete detection of a plurality of samples within 30 minutes. Small volume and portability. The temperature control precision is accurate. A software/hardware double-layer over-temperature protection function is built in, so that the stability of operation and the safety under unattended operation are ensured. The optical sensing is designed to be error proof and may sense whether an agent is placed in the device.

Of course, it is not necessary for any particular product to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a isothermal nucleic acid amplification detection apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of the back side of a nucleic acid amplification isothermal detection apparatus according to an embodiment of the present invention;

FIG. 3 is a block diagram showing the connection of the components included in the isothermal nucleic acid amplification detecting apparatus according to the embodiment of the present invention.

In the figure: the device comprises a shell 1, a hot cover 2, a hot cover heating plate 21, a groove 211, a constant temperature unit 3, a heating assembly 31, a temperature sensor 32, a detection unit 4, a background light-emitting lamp 41, a color sensor 42, a control unit 5, a switch 6, a bolt 7, a lock hole 8, a display panel 9, a touch power switch 10, a power socket 11, a heat insulation bottom plate 12 and a PCR test tube 13.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art all belong to the protection scope of the present invention.

Examples

Referring to fig. 1, fig. 2, and fig. 3, a constant temperature detection device for nucleic acid amplification according to an embodiment of the present invention, as shown in fig. 1, fig. 2, and fig. 3, the device may include:

a housing 1, the housing 1 being connected to a thermal cover 2;

the constant temperature unit 3 is connected with the shell 1; the thermostatic unit 3 comprises a heating assembly 31 and a temperature sensor 32, wherein the heating assembly 31 comprises a plurality of pipe holes for accommodating the PCR test tubes 13; the number of the pipe holes can be determined according to needs, for example, 4 pipe holes can be arranged. 4 tubes can be simultaneously tested for amplification each time.

The detection unit 4, the said detection unit 4 couples to said body 1; the detection unit 4 comprises a background light-emitting lamp 41, a color sensor 42 and quartz glass;

the control unit 5 is connected with the shell 1, and the constant temperature unit 3 and the detection unit 4 are both in communication connection with the control unit 5;

the control unit 5 is configured to receive the temperature value obtained by the temperature sensor 32 and the color data value obtained by the color sensor 42, so as to control the heating assembly to provide a constant temperature to the PCR tube 13 according to the temperature data value and generate an interpretation result according to the color data value.

The isothermal nucleic acid amplification detection device provided by the embodiment of the application is based on reverse transcription loop-mediated isothermal amplification and optical color development detection interpretation technology. The device can provide constant temperature conditions for nucleic acid amplification, the detector receives a reagent color development signal, and the system automatically interprets and displays the signal. The shell is arranged to bear all the components required by heating and detection, so that the device is high in mobility and particularly suitable for amplification detection of a small number of samples. The device is small and exquisite in whole equipment, can be carried about by a user, is high in equipment integration degree, and can realize automatic amplification and detection after sampling.

It can be understood that the constant temperature unit that this application embodiment provided can heat the test tube, and temperature data collection and the accurate control of the control unit through temperature sensor can be so that the inside of casing is in the constant temperature state all the time. In order to better keep warm to the casing inside to and improve the constant temperature effect, this application embodiment can also provide hot lid 2 includes hot lid hot plate 21, hot lid hot plate is provided with recess 211, recess 211 is used for setting up hot lid heating element, hot lid hot plate is got rid of recess 211 part is provided with insulation material. Place heating element in the recess on the hot lid hot plate 21, set up the recess one purpose and hold test tube 13, another purpose can avoid heating element and hot lid hot plate 21 to go up the contact of heat preservation material and cause ageing or come unstuck. Heating element can guarantee that the test tube part under one's side is in the constant temperature state all the time, and the heat that hot lid hot plate produced can provide the required heat of constant temperature for test tube upper portion, guarantees that the reagent in the test tube obtains more excellent constant temperature environment.

It can be understood that, in order to improve the device and prevent that hot lid from causing the scald to the user when using, this application embodiment can also provide prevents scalding the module, prevents that the heating element on the hot plate of hot lid from causing the scald to the people, prevents scalding the module and can open 2 back recesses 211 of hot lid and have the automatic closure of baffle, seals heating element, perhaps opens 2 backs of hot lid 2 that hot lid 2 twists reverse 180 degrees, makes heating element down, is difficult for being touched.

The thermal cover according to the embodiment of the present application may be connected to the housing in various ways, for example, in one implementation, the thermal cover 2 may be hinged to the housing 1, so that the thermal cover 2 may be opened reversely relative to the housing 1, and the opening angle of the thermal cover 2 is not less than 90 degrees. The mode that the hot cover is hinged with the shell is adopted, so that the opening of the hot cover is facilitated, and the integral integration of the device is improved. The opening angle is not less than 90 degrees, so that the test tube can be placed in and taken out conveniently.

For the convenience that the hot lid of further improvement was opened and was closed, this application embodiment can also provide hot lid 2 with the articulated department of casing 1 is provided with the torsional spring, hot lid 2 is provided with switch 6 and spring bolt 7, be provided with on the casing 1 with the lockhole 8 of spring bolt 7 adaptation. The switch through setting up can drive the spring bolt action for the spring bolt is popped out in by the lockhole, can guarantee through the torsional spring that sets up that the spring bolt pops out the back, and the hot lid can upwards turn over automatically and open.

The effect of the heating element that this application embodiment provided is for the test tube provides the required heat of constant temperature, and temperature sensor is used for acquireing corresponding partial real-time temperature value, and this heating element and temperature sensor all can adopt multiple form, for example, under an implementation, this application embodiment can provide heating element 31 includes the film heating piece, temperature sensor 32 includes the resistance temperature sensor of platinum material. The curved film heating sheet has the characteristic of being bendable, uses a metal film as a heating element, and innovatively manufactures a bendable, ultrathin and heating film. Be applied to in the device that this application provided, can guarantee according to pipe hole shape and position requirement bending become can be better with the test tube laminating, reach heat transmission's optimal state. The platinum resistance temperature sensor measures temperature by utilizing the characteristic that the resistance value of the platinum changes along with the temperature change of the platinum, and the display instrument indicates the temperature value corresponding to the resistance value of the platinum resistance. When a temperature gradient exists in the measured medium, the measured temperature is the average temperature in the medium layer in the range of the temperature sensing element. The platinum resistance temperature sensor is a temperature sensor manufactured by utilizing a certain functional relation between the resistance and the temperature. Since the characteristic curve of the resistance of the platinum material is nonlinear, the standard resistance-temperature relationship is given in the form of a scale and is expressed by a resistance-temperature polynomial function r (t). However, in actual measurement, the temperature-resistance function T (r) is used for facilitating measurement and calculation. A method is presented for obtaining a temperature-resistance polynomial function t (r) using cubic basic spline curve fitting. Practical use shows that the method has high calculation speed and small generated error, and can well improve the operation speed and the control precision of the temperature measurement and control system.

In order to display the real-time temperature obtained by the sensor and the generated interpretation result to the user in real time and facilitate the observation of the user, the embodiment of the application may further provide that a display panel 9 is disposed on the front end face of the housing, and the display panel 9 is in communication connection with the control unit 5. The display panel can display the temperature value and the judgment result for a user to check.

In order to further improve the integration degree of the device, the embodiment of the present application may further provide that the front end surface of the housing 1 is provided with a touch power switch 10. The rear end face of the shell is provided with a power socket 11. The bottom of the housing 1 is provided with a heat insulating bottom plate 12. The power socket can adopt a universal socket structure, and the power socket is ensured to be more convenient to obtain power. The heat insulation bottom plate can effectively insulate heat inside the shell.

In order to detect whether the test tube is placed in the tube hole, an optical sensor (not shown) may be further provided in the embodiment of the present application, the optical sensor is connected to the housing, the optical sensor is communicatively connected to the control unit, and the control unit is further configured to determine whether the PCR test tube is placed in the tube hole according to information obtained by the optical sensor.

The application provides a nucleic acid amplification constant temperature detection device comprises casing, hot lid and bottom plate. The hot lid inboard loads has hot lid hot plate, and hot lid can be opened for casing 90 degrees, and touch switch is in the casing dead ahead, and external power source connector distributes at the casing back. The supporting detecting element of equipment, temperature control unit, display panel part.

The constant temperature unit can be composed of a film heating sheet, a temperature sensor (platinum resistor) and an MCU processor circuit, and the PID temperature control algorithm is adopted to adjust the heating power of the film heating sheet in real time according to the module temperature value fed back by the temperature sensor, so that constant temperature control is realized, and a stable constant temperature environment is provided for the amplification reaction.

The detection unit structure comprises a background luminescent lamp, a color sensor and quartz glass, and the basic principle is that a beam of white light is irradiated on a test tube through the luminescent lamp, the color sensor reads color change on the test tube in real time, and interpretation is made according to the color change characteristic of a reagent.

Constant temperature unit and detecting element module all directly link the MCU treater, and the MCU treater reads the temperature value in real time and output control signal adjustment heating plate heating power, also reads the data of 4 way color sensor in the detecting element in real time simultaneously, and the RGB data through the color sensor feedback makes the interpretation. The display panel is controlled by the MCU processor and displays the current working state, real-time temperature value, interpretation result and the like of the whole machine.

In a word, the isothermal nucleic acid amplification detection device provided by the application supports real-time interpretation and countdown display, and can complete detection of a plurality of samples within 30 minutes. Small volume and portability. The temperature control precision is accurate. A software/hardware double-layer over-temperature protection function is built in, so that the stability of operation and the safety under unattended operation are ensured. The optical sensing is designed to be error proof and may sense whether an agent is placed in the device.

It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims

1. A isothermal detection device for nucleic acid amplification, comprising:

a housing to which a thermal cover is connected;

the control unit is used for receiving the temperature value acquired by the temperature sensor and the color data value acquired by the color sensor, so as to control the heating assembly to provide constant temperature for the PCR test tube according to the temperature value and generate an interpretation result according to the color data value.

2. The isothermal nucleic acid amplification detection device according to claim 1, wherein the heat cover comprises a heat cover heating plate provided with a groove for providing a heat cover heating element, and the heat cover heating plate is provided with a heat insulating material except the groove portion.

3. The isothermal nucleic acid amplification detection device according to claim 2, wherein the thermal cover is hinged to the housing so that the thermal cover can be opened in a reverse direction with respect to the housing, and an opening angle of the thermal cover is not less than 90 degrees.

4. The nucleic acid amplification constant temperature detection device of claim 3, wherein a torsion spring is arranged at the hinged position of the thermal cover and the shell, the thermal cover is provided with a switch and a lock tongue, and the shell is provided with a lock hole matched with the lock tongue.

5. The isothermal nucleic acid amplification detection device of claim 1, wherein the heating assembly comprises a thin film heater chip and the temperature sensor comprises a resistance temperature sensor.

6. The isothermal nucleic acid amplification detection device according to claim 1, wherein a display panel is disposed on a front end surface of the housing, and the display panel is communicatively connected to the control unit.

7. The isothermal nucleic acid amplification detection device according to claim 1, wherein a touch power switch is provided on a front end surface of the housing.

8. The isothermal nucleic acid amplification detection device according to claim 1, wherein a power jack is provided on a rear end surface of the housing.

9. The isothermal nucleic acid amplification detection device according to claim 1, wherein a heat insulating bottom plate is provided at the bottom of the housing.

10. The isothermal nucleic acid amplification detection device according to claim 1, further comprising an optical sensor, wherein the optical sensor is connected to the housing, the optical sensor is communicatively connected to the control unit, and the control unit is further configured to determine whether the PCR cuvette is placed in the cuvette hole according to information obtained by the optical sensor.