CN217385193U - Sterilizable ultramicro ultraviolet spectrophotometer - Google Patents
Sterilizable ultramicro ultraviolet spectrophotometer Download PDFInfo
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- CN217385193U CN217385193U CN202221092387.4U CN202221092387U CN217385193U CN 217385193 U CN217385193 U CN 217385193U CN 202221092387 U CN202221092387 U CN 202221092387U CN 217385193 U CN217385193 U CN 217385193U
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Abstract
The application relates to a sterilizable ultramicro ultraviolet spectrophotometer, which comprises a body, a sample seat arranged on the body and used for containing a sample, a sample arm connected with the sample seat or the body and capable of rotating relative to the sample seat, a light source arranged on the body and used for emitting light, a receiver with the light source respectively arranged in the sample seat and the sample arm, and a sterilizing piece arranged on the sample arm or the sample seat and used for sterilizing the sample seat and the sample arm, wherein when the sample arm rotates relative to the sample seat to be abutted against the sample seat, a liquid column is formed between the sample arm and the sample seat by a sample; the receiver is used for receiving the light absorbed by the liquid column. The application provides an ultramicro ultraviolet spectrophotometer has the disinfection piece, after sample detection, uses the paper handkerchief to clean the sample clean back, starts the disinfection piece and disinfects sample seat and sample arm, gets rid of the sample and remains to avoid causing the pollution to next sample detection, improve and detect the precision.
Description
The present application claims priority from chinese patent application having application number 202122019549.3, application number 2021, 8/25, the entire contents of which are incorporated herein by reference.
Technical Field
The utility model relates to a detecting instrument technical field especially relates to a can sterile ultramicro ultraviolet spectrophotometer.
Background
The detection technology is one of the indispensable means of scientific experiments, and the commonly used ultraviolet-visible spectrophotometry is a qualitative, quantitative and structural analysis method established according to the absorption characteristics of substance molecules to electromagnetic waves with different ranges of wavelengths. The ultramicro-UV spectrophotometer is widely applied because of its advantages of rapid detection, no damage to protein, no sample consumption (the sample consumption is generally 0.5-2 microliter).
After the existing ultramicro ultraviolet spectrophotometer detects a sample, the sample is wiped clean by using a paper towel, but the sample is remained on the instrument by the operation, so that the pollution to the next sample detection is caused, and the precision of the detection result is reduced.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a but detect sterile ultramicro ultraviolet spectrophotometer that the precision is high.
In order to achieve the above purpose, the utility model provides a following technical scheme: a sterilizable ultramicro uv spectrophotometer comprising:
a body;
the sample seat is arranged on the machine body and used for containing a sample;
the sample arm is connected with the sample seat or the body and can rotate relative to the sample seat, and when the sample arm rotates relative to the sample seat to abut against the sample seat, the sample forms a liquid column between the sample arm and the sample seat;
the light source is arranged on the machine body and used for emitting light;
the receiver and the light source are respectively arranged in the sample seat and the sample arm, and the receiver is used for receiving the light rays absorbed by the liquid column.
A sterilizing member disposed on the sample arm or sample holder to sterilize the sample holder and sample arm.
Furthermore, a first optical fiber is arranged on the sample seat, a second optical fiber is arranged on the sample arm, and one of the light source and the receiver is connected with the first optical fiber; the other of the light source and the receiver is connected to the second optical fiber.
Further, a first platform is formed on the sample holder, the first platform is used for containing a sample, and the first optical fiber is at least partially arranged in the first platform.
Further, a second platform is formed on the sample arm, the second optical fiber is at least partially disposed within the second platform, and the fluid column is formed between the first platform and the second platform.
Further, the disinfection piece is an ultraviolet lamp.
Further, a first groove is formed in one side, facing the sample arm, of the sample seat, and the first platform and the ultraviolet lamp are oppositely located at two ends in the first groove;
or a second groove is formed in one side, facing the sample seat, of the sample arm, and the second platform and the ultraviolet lamp are located at two ends of the second groove relatively.
Further, the light source is a pulse xenon lamp.
Further, the receiver is a CCD array.
Further, the sterilizable ultramicro uv spectrophotometer further comprises a display device connected with the receiver to display a detection spectrogram of the sample.
Further, the sample arm and the sample holder are pivotally connected.
The beneficial effects of the utility model reside in that: the application provides an ultramicro ultraviolet spectrophotometer has the disinfection piece, after finishing to sample testing, uses the paper handkerchief to clean the sample back, starts the disinfection piece and disinfects sample seat and sample arm, gets rid of the sample and remains to avoid causing the pollution to next sample testing, improve and detect the precision.
The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings.
Drawings
Fig. 1 is a schematic structural view of a sterilizable ultramicro uv spectrophotometer according to an embodiment of the present application.
Fig. 2 is a schematic view of a detection light path.
The system comprises a machine body 1, a sample seat 2, a first platform 21, a sample arm 3, a second platform 31, a disinfection piece 4, a first groove 5, a pulse xenon lamp 6, a filter 7, a prism 8, a slit 9, a receiver 10 and a display device 11.
Detailed Description
The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the mechanism or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Furthermore, the technical features mentioned in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
Referring to fig. 1, a sterilizable ultramicro uv spectrophotometer according to an embodiment of the present application includes a body 1, a light source (not shown) and a sample holder 2 disposed on the body 1, and a sample arm 3 connected to the sample holder 2 or the body 1.
The machine body 1 is of a cuboid-like structure, the size of the machine body 1 is small, the occupied area is small, and the carrying is convenient. The sample holder 2 may be fixed to the body 1, or may be integrally formed with the body 1, and is not limited herein. The sample holder 2 is used for holding a sample, that is, when the sample needs to be detected, the sample is dripped onto the sample holder 2. Specifically, the sample holder 2 is formed with a first platform 21, and the sample is placed on the first platform 21.
In this embodiment, the sample arm 3 is disposed on the sample holder 2 and can rotate relative to the sample holder 2, and in other embodiments, the sample arm 3 can be disposed on the machine body 1.
The sample arm 3 and the sample holder 2 are pivotally connected, and the specific pivoting manner is the conventional structure, which is not described herein again.
The sample arm 3 is formed with a second platform 31, which can abut against the sample holder 2 after the sample arm 3 rotates towards the sample holder 2, and at this time, the first platform 21 and the second platform 31 are oppositely disposed, and the second platform 31 faces the sample placed on the first platform 21. The liquid tension is utilized to form a liquid column between the sample arm 3 and the sample holder 2, and the distance between the first platform 21 and the second platform 31 can be set according to actual needs.
The sterilizable ultramicro uv spectrophotometer further comprises a receiver (not shown) and a display device (not shown) connected to the receiver. The light source is used for emitting light rays, the light rays irradiate the sample, and the receiver is used for receiving the light rays absorbed by the liquid column formed by the sample. The receiver and the light source are arranged in the sample holder and the sample arm, respectively. That is, one of the receiver and the light source is disposed in the sample holder, and the other of the receiver and the light source is disposed in the sample arm, so that the receiver and the light source are disposed at both ends of the liquid column, respectively.
The receiver is a CCD array, i.e. a charge coupled device. The receiver converts the optical signal into an electrical signal, which can be transmitted to a display device and can display a detected spectrogram of the sample on the display device.
The connection mode of the receiver and the display device may be a wired connection or a wireless connection, and is not limited in particular.
The display device can be a computer or a panel, corresponding software is installed on the display device to control the operation of the sterilizable ultramicro ultraviolet spectrophotometer, the software is divided into a left block and a right block, a status bar and a working key are arranged on the left side, and a main menu and data are displayed on the right side. According to the toolbar option, a specific application can be opened, and the software stores the corresponding detection method and can also be called for use. The specific setting of the software is related to the prior art, and is not described in detail herein.
A first optical fiber (not shown) is arranged on the sample holder 2, a second optical fiber (not shown) is arranged on the sample arm 3, and one of the light source and the receiver is connected with the first optical fiber; the other of the light source and the receiver is connected to a second optical fiber.
In this embodiment, the light source is connected to the first optical fiber; the receiver is connected with the second optical fiber, the first optical fiber is used as a light source transmitting end, and the second optical fiber is used as an optical signal receiving end. Light of the light source irradiates on a liquid column formed by the sample through the first optical fiber, and after being absorbed by the sample, the light irradiates on the second optical fiber and is transmitted to the receiver.
In particular, the first optical fiber is at least partially arranged in a first platform 21, i.e. in the sample holder 2 embedded in the first optical fiber, with one end of the first optical fiber arranged close to the first platform 21. The second optical fiber is disposed at least partially within the second platform 31, with one end of the second optical fiber also disposed proximate the second platform 31.
When the sample arm 3 and the sample holder 2 are abutted, the first platform 21 serves as a light source emitting end, the second platform 31 serves as a light signal receiving end, and a trace liquid column is formed between the two end faces by using liquid tension to form an absorption pool.
In this embodiment, the light source is a pulsed xenon lamp, and the deuterium lamp is the most ideal ultraviolet light source currently used in the ultraviolet spectrophotometer 100, and the applicable wavelength range is 185-400 nm. When the deuterium lamp works, the maximum value of the light source energy is near 230nm, and two characteristic spectral lines of 486.0nm and 656.1nm exist in a visible region, so that the deuterium lamp can be used for correcting the wavelength accuracy of an instrument.
Referring to fig. 2, a light source generating assembly composed of a pulse xenon lamp 6, a filter 7, a prism 8 and a slit 9 is disposed inside a sample holder 2 and connected to a first optical fiber, a receiver 10 is disposed inside a sample arm and connected to a second optical fiber, and a display device 11 can be externally connected to a machine body 1 or disposed on the machine body 1. The light generated by the pulse xenon lamp 6 irradiates on a filter 7, the filter 7 collects the light and transmits the light through a prism 8, the prism 8 divides the light collected by the filter 7 into a plurality of component wavelengths, then the light with specific wavelengths is selectively transmitted through a slit 9, the slit 9 selectively transmits the light with the specific wavelengths and irradiates on a liquid column between a first platform and a second platform through a first optical fiber, the light absorbed by the liquid column and irradiates on a receiver 10 through a second optical fiber, the receiver 10 measures the amount of the collected photons and converts the light signals into electric signals and transmits the electric signals to a display device 11. The concentration of the sample can be calculated by comparing the photon quantity before and after the sample is transmitted and calculating by the beer-Lambert law. It should be noted that this part belongs to the prior art and is not described in detail here.
Since bases (G, A, T, C) constituting a nucleic acid have a strong absorption peak at 260nm, DNA can be quantified by measuring the absorption peak at 260 nm.
After the sample is detected, the sample is wiped clean by clean dust-free paper, so as to avoid the pollution to the next detected sample, the sterilizable ultramicro ultraviolet spectrophotometer also comprises a sterilizing piece 4, and the sterilizing piece 4 is arranged on the sample arm 3 or the sample seat 2 so as to sterilize the sample seat 2 and the sample arm 3.
The sterilizing member 4 is an ultraviolet lamp 4, but is not limited thereto, and the sterilizing member 4 may also be an apparatus for generating ozone, etc., which are not listed here.
Specifically, a second groove 5 is formed on one side of the sample arm 3 facing the sample holder 2, and the second platform 31 and the ultraviolet lamp 4 are located at two opposite ends of the second groove 5. It should be noted that the ultraviolet lamp 4 is disposed on one side of the second platform 31 and is disposed toward the second platform 31, that is, the light of the ultraviolet lamp 4 irradiates in the direction toward the second platform 31.
After the sample is detected, the samples on the first platform 21 and the second platform 31 are wiped clean with clean dust-free paper, and then the sample arm 3 is covered on the sample holder 2. And starting the ultraviolet lamp 4, and irradiating ultraviolet light generated by the ultraviolet lamp 4 to the second platform 31 through the second groove 5. Because a micro-liquid column is formed between the two end faces of the first platform 21 and the second platform 31, a gap is formed between the first platform 21 and the second platform 31, and ultraviolet light irradiates the first platform 21 and the second platform 31 through the second groove 5 to sterilize the first platform 21 and the second platform 31, so that the residue of a sample is eliminated, for example, the residue of a molecular structure of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) in the sample can be removed, and the detection precision is improved.
Alternatively, or in addition, the sample holder 2 is provided with a first groove (not shown) on the side facing the sample arm 3, and the first platform 21 and the ultraviolet lamp 4 are oppositely positioned at two ends in the first groove. Similarly, the ultraviolet light emitted from the ultraviolet lamp 4 is irradiated onto the first stage 21 and the second stage 31 through the first recess.
In another embodiment, a first groove may be provided on the sample holder 2, a second groove 5 may be provided on the sample arm 3, and the first groove and the second groove 5 are communicated when the sample arm 3 is covered on the sample holder 2. The uv lamp 4 may be disposed in the first recess or in the second recess 5.
In other embodiments, the sample arm 3 or the sample holder 2 is further made of a transparent material, so that the light emitted from the ultraviolet lamp 4 disposed on the sample arm 3 or the sample holder 2 passes through the transparent material and irradiates the first platform 21 and the second platform 31 to sterilize them.
The spectrophotometer 100 has the advantages of small occupied area, light weight, plug and play and simple structure; the method can directly measure samples such as high-concentration nucleic acid (deoxyribonucleic acid DNA or ribonucleic acid RNA) and the like, does not need to dilute, and can directly measure the purity and the concentration of the samples; the cost is low, the amount of the sample measured each time is only 0.5-2.0 mu L, and the waste of valuable or scarce samples is avoided; the operation is simple, the time consumption is short, and only the sample needs to be directly sucked and moved onto the sample seat 2; has wide dynamic range, can measure 2-3700 ng/mu L (dsDNA); the use of a cuvette is avoided; the measuring speed is high, and the efficiency is higher than that of the ultraviolet absorption method of the fluorescent dye method.
Since bases (G, A, T, C) constituting a nucleic acid have a strong absorption peak at 260nm, DNA can be quantified by measuring the absorption peak at 260 nm. The other light-absorbing substance most frequently present in nucleic acid samples is protein, and since protein has a strong absorption peak at 280nm, the purity of DNA can be judged by measuring the A260/A280 ratio. In practical use of this embodiment, when the nucleic acid concentration is detected, the wavelength is adjusted by the wavelength adjustment button, and the wavelength display window on the display device 11 is observed and adjusted to the desired measurement wavelength. A nucleic acid detection sample to be detected is respectively added on the first platform 21 by using a pipette, the sample arm 3 is buckled, the distance between the first platform 21 and the second platform 31 is adjusted, a liquid column is formed between optical fibers arranged on the first platform 21 and the second platform 31, the detection light with a specific wavelength emitted by the first optical fiber embedded on the first platform 21 is received by the receiver 10 through the nucleic acid detection sample and is converted into an electric signal to be transmitted to the display device 11. When the detection is finished, the software installed in the display device 11 will display the full wavelength curve and the detection result. It should be noted that after each sample is detected, the first platform 21 and the second platform 31 are wiped by clean dust-free paper, the sample is wiped completely, then the sample arm 3 is buckled, the ultraviolet lamp 4 is turned on, and ultraviolet light emitted by the ultraviolet lamp 4 irradiates the first platform 21 and the second platform 31 through the second groove 5, so that the sample residue is eliminated and the next detection is performed.
In summary, the ultramicro-uv spectrophotometer provided by the present application has a disinfecting member, after the sample is detected, the paper towel is used to wipe the sample clean, the disinfecting member is started to disinfect the sample holder and the sample arm, and the molecular structure residue of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) in the sample is removed, so that the pollution to the next sample detection is avoided, and the detection precision is improved.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the concept of the present invention, several variations and modifications can be made, which all fall within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (10)
1. A sterilizable ultramicro uv spectrophotometer comprising:
a body;
the sample seat is arranged on the machine body and used for containing a sample;
the sample arm is connected with the sample seat or the body and can rotate relative to the sample seat, and when the sample arm rotates relative to the sample seat to abut against the sample seat, the sample forms a liquid column between the sample arm and the sample seat;
the light source is arranged on the machine body and used for emitting light;
the receiver and the light source are respectively arranged in the sample seat and the sample arm, and the receiver is used for receiving the light absorbed by the liquid column;
a sterilizing member disposed on the sample arm or sample holder to sterilize the sample holder and sample arm.
2. The sterilizable ultramicro uv spectrophotometer according to claim 1, wherein a first optical fiber is provided on the sample holder, a second optical fiber is provided on the sample arm, and one of the light source and the receiver is connected to the first optical fiber; the other of the light source and the receiver is connected to the second optical fiber.
3. The sterilizable ultramicro uv spectrophotometer of claim 2, wherein said sample holder has a first platform formed thereon for holding a sample, said first optical fiber being at least partially disposed within said first platform.
4. The sterilizable ultramicro uv spectrophotometer of claim 3, wherein the sample arm has a second platform formed thereon, the second optical fiber being at least partially disposed within the second platform, the liquid column being formed between the first platform and the second platform.
5. The sterilizable ultramicro uv spectrophotometer of claim 4, wherein the sterilization member is an ultraviolet lamp.
6. The sterilizable ultramicro uv spectrophotometer according to claim 5, wherein a first recess is provided on a side of said sample holder facing said sample arm, said first platform and said uv lamp being located at opposite ends in said first recess;
or a second groove is formed in one side, facing the sample seat, of the sample arm, and the second platform and the ultraviolet lamp are located at two ends of the second groove relatively.
7. The sterilizable ultramicro uv spectrophotometer according to claim 1, wherein the light source is a pulsed xenon lamp.
8. The sterilizable ultramicro uv spectrophotometer of claim 1, wherein the receiver is a CCD array.
9. The sterilizable ultramicro uv spectrophotometer of claim 1, further comprising a display device connected to the receiver to display a detection spectrum of a sample.
10. The sterilizable ultramicro uv spectrophotometer of claim 1, wherein said sample arm and said sample holder are pivotally connected.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2021220195493 | 2021-08-25 | ||
| CN202122019549 | 2021-08-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217385193U true CN217385193U (en) | 2022-09-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221092387.4U Active CN217385193U (en) | 2021-08-25 | 2022-05-09 | Sterilizable ultramicro ultraviolet spectrophotometer |
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| CN (1) | CN217385193U (en) |
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- 2022-05-09 CN CN202221092387.4U patent/CN217385193U/en active Active
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