CN217340170U

CN217340170U - A adsorption column supporting component for plasmid is drawed

Info

Publication number: CN217340170U
Application number: CN202221090908.2U
Authority: CN
Inventors: 王婷; 王飞; 陈冰; 马万里; 曹蒙蒙; 刘芸; 石琳; 王永增; 吕璐璐
Original assignee: Heyuan Biotechnology Tianjin Co ltd
Current assignee: Heyuan Biotechnology Tianjin Co ltd
Priority date: 2022-05-09
Filing date: 2022-05-09
Publication date: 2022-09-02
Anticipated expiration: 2032-05-09

Abstract

The utility model relates to an adsorption column supporting component for plasmid draws, include: a support frame and a diameter adjusting piece. Wherein, the support frame includes roof, bottom plate and riser. An opening is arranged on the top plate. Wherein the diameter adjusting piece is provided with an opening for being superposed on the top plate, the opening of the diameter adjusting piece is concentric with the opening of the top plate, and the opening of the diameter adjusting piece is smaller than the opening of the top plate. The adsorption column support assembly is designed to be able to hold the adsorption column and to accommodate a solution collection container disposed below the adsorption column in a use state. The utility model discloses an adsorption column supporting component can support the adsorption column of different diameter sizes effectively to have that the equipment is simple, stability is good, difficult toppling, disinfection and clean convenient characteristics.

Description

A adsorption column supporting component for plasmid is drawed

Technical Field

The utility model relates to an adsorption column supporting component for plasmid draws especially relates to the adsorption column supporting component that can support the adsorption column of different diameters.

Background

In the field of genetic engineering, plasmids are a common gene delivery vehicle. In particular, the plasmid is capable of carrying foreign genes into bacteria and amplifying or expressing in bacteria. Therefore, the efficient plasmid extraction method has extremely high application value.

The plasmid extraction methods common in the prior art are usually accomplished with the aid of kits and take the following specific principles: in a strongly alkaline environment, the cell wall and cell membrane of the bacterium are destroyed, and chromosomal DNA and plasmid DNA are released. Wherein the double helix structure of the chromosomal DNA is denatured by destruction in a strong alkaline environment. Although the plasmid DNA in a covalently closed circular form is also denatured in a strong alkaline environment, the two complementary strands of the plasmid DNA still coil and are tightly bound to each other. Plasmid DNA in a covalently closed circular form renatures rapidly, while chromosomal DNA in a linear form renatures slowly, when the environment is brought back to neutrality by the addition of high-salt buffer solutions. The protein, the disrupted cell wall and the denatured chromosomal DNA are then intertwined with each other to form a large complex, thereby achieving the technical effect of separating plasmid DNA in a covalently closed circular form from chromosomal DNA in a linear form. Then, the plasmid is adsorbed by adopting an adsorption column, and the finished product plasmid can be obtained by the procedures of impurity removal, elution and the like.

Adsorption is a very important step during the specific handling of the kit. The purpose to be achieved by the adsorption is to specifically bind nucleic acids to silicon substrates, thereby facilitating the removal of impurities such as proteins, disrupted cell walls and chromosomal DNA. In order to achieve adsorption, the operator first needs to vertically suspend and position the adsorption column attached to the kit, and continuously add the solution to be treated to the adsorption column. The inside of the adsorption column is in a cylindrical shape, and the outside of the column body is provided with a clamping part (such as a thin edge) which is easy to clamp, so that the adsorption column is convenient to clamp on a supporting facility when in use, and thus the solution in the adsorption column flows out from the lower part of the adsorption column under the action of gravity and flows into a solution collecting container arranged below the adsorption column. The existing kit is not provided with a support frame for vertically suspending and positioning the adsorption column, so that an operator is required to assemble the temporary support frame by himself. Because plasmid extraction is carried out in a biosafety cabinet, the instruments (such as a gun tip box and a test tube rack) which can be selected are limited when the temporary support frame is assembled every time. Meanwhile, in order to ensure that there is enough space to collect the solution, the assembling apparatus is generally required to be stacked, so that the stability of the assembled stand is low. This further increases the risk of tipping of the support frame, thereby causing experimental failure. Furthermore, the instruments used to assemble the temporary support are generally difficult to disinfect and clean, which raises the risk of contamination of the experiment from the side, which is detrimental to the safe performance of the experiment as a whole. In addition, the diameter dimensions of the adsorption columns used in the adsorption operation are not generally uniform, so that the temporary support frames need to be assembled separately for adsorption columns of different diameters, which greatly reduces the efficiency of the experiment.

Therefore, it is necessary to develop an adsorption column support facility for plasmid extraction capable of supporting adsorption columns of different diameters so as to be capable of effectively supporting adsorption columns of different diameter sizes, and having the characteristics of simple assembly, good stability, difficulty in overturning, and convenience in sterilization and cleaning.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an adsorption column supporting component for plasmid is drawed to overcome prior art's problem.

To this end, the utility model provides an adsorption column supporting component (also simply called adsorption column supporting component hereinafter) for plasmid is drawed, include:

a support frame, and

a diameter adjustment member;

the supporting frame comprises a top plate, a bottom plate and a vertical plate, wherein the top plate and the bottom plate are parallel to each other and are horizontally arranged, the vertical plate is perpendicular to the top plate and the bottom plate, the top plate and the bottom plate are fixed on the same side of the vertical plate, and an opening is formed in the top plate; wherein the diameter adjusting piece is provided with an opening for being superposed on the top plate, the opening of the diameter adjusting piece is concentric with the opening of the top plate, and the opening of the diameter adjusting piece is smaller than the opening of the top plate.

The utility model discloses an adsorption column supporting component can support the adsorption column of different diameter sizes effectively to have that the equipment is simple, stability is good, difficult toppling, disinfection and clean convenient characteristics.

Drawings

The advantages, features of the present invention will now be described in detail with reference to the accompanying drawings, in which the components are not necessarily drawn to scale, and wherein:

figure 1 shows a perspective view of one embodiment of an adsorption column support assembly.

Fig. 2 shows a cross-sectional view of a front view of one embodiment of the sorption column support assembly shown in fig. 1, wherein the sorption column support assembly is cut along a vertical plane through a centerline of the sorption column support assembly.

Figure 3 shows a top view of one embodiment of the sorption column support assembly shown in figure 1.

Figure 4 shows a reference view of a state of use of one embodiment of the sorption column support assembly shown in figure 1.

Figure 5 shows a perspective view of another embodiment of an adsorption column support assembly.

Fig. 6 shows a cross-sectional view of a front view of another embodiment of the sorption column support assembly shown in fig. 5, wherein the sorption column support assembly is cut along a vertical plane through a centerline of the sorption column support assembly.

FIG. 7 is a partial view of FIG. 6, particularly illustrating the details of the connection of the diameter adjuster to the top plate.

Figure 8 shows a top view of another embodiment of the sorption column support assembly shown in figure 5.

Fig. 9 is a reference view showing a state of use of another embodiment of the adsorption column support assembly shown in fig. 5.

It is to be understood that the drawings are drawn for purposes of illustration only and are not to be construed as limiting the invention.

Detailed Description

In this specification, the terms "bottom, top, upward, downward" are divided by the orientation of the adsorbent column support assembly shown in fig. 1. That is, the positioning and orientation of the adsorption column support assembly according to the present invention when in use will be described.

The utility model discloses an adsorption column supporting component for plasmid draws includes:

a support frame, and

a diameter adjustment member;

Obviously, after passing the bottom of the adsorption column for plasmid extraction through the opening of the top plate, the clamping part of the adsorption column can be "clamped" on the top plate, so as to be supported on the adsorption column supporting component of the present invention. If the diameter of the clamping position of the adsorption column is significantly smaller than the opening diameter of the top plate, adsorption columns with different diameters can be supported on the adsorption column supporting component by means of the diameter adjusting piece. Therefore, the diameter-adjusting member may be plural, each opening having a different diameter. Meanwhile, the solution collecting container is arranged on the bottom plate and used for collecting liquid flowing out of the adsorption column.

Based on the utility model discloses an adsorption column supporting component's structure and usage, it can be understood that "bottom plate" except being independent plate, can also be the panel that existing test bench or supporting mechanism's level set up, as long as with the utility model discloses a riser is connected, can be together with roof, diameter adjustment spare, constitutes the utility model discloses an adsorption column supporting component, these all belong to the utility model discloses a scope.

In a preferred embodiment of the present invention, the diameter-adjusting member is in the form of a sheet.

In a preferred embodiment of the invention, the riser is connected at one end (not necessarily the end) to the bottom plate and at the other end (not necessarily the end) to the top plate.

According to the utility model discloses an adsorption column supporting component for plasmid extraction, preferably, the opening of diameter adjusting part with opening on the roof is the form of circular opening, wherein diameter adjusting part with be provided with between the roof and be used for injecing the stop gear of diameter adjusting part relative roof position. More preferably, the top plate is provided with a straight groove extending from the edge of the top plate to the center of the circular opening of the top plate, and the center line of the straight groove extends through the center of the circular opening of the top plate. The straight groove is matched with the leakage part of the adsorption column, so that the technical effects of guiding the adsorption column and facilitating installation are achieved.

According to an adsorption column supporting component for plasmid draws, preferably, still be provided with ascending the buckling on the roof, the line of buckling with the edge of diameter adjustment piece is tangent, thereby constitutes stop gear. More preferably, the included angle between the horizontal portion and the bent portion of the top plate ranges from 120 degrees to 170 degrees. Since the edge of the diameter adjustment member is tangent to the bend line, this defines the position of the diameter adjustment member on the top plate, thereby achieving the technical effect of enhancing the stability of the support.

According to the utility model discloses an adsorption column supporting component for plasmid extraction, preferably, the diameter regulating part is the form of step shaft in vertical cross-section, the circular opening of diameter regulating part with the step shaft is concentric, the step shaft includes first festival section and second festival section, the external diameter less than or equal to of first festival section the circular open-ended diameter of roof, the external diameter of second festival section is greater than the circular open-ended diameter of roof, the first festival section of diameter regulating part passes the circular open-ended of roof, thereby constitute stop gear. That is, the diameter-adjusting member of this structure is fixed in position relative to the top plate when in use, thereby achieving a technical effect of further enhancing the stability of the support. More preferably, a protrusion is provided at an end surface of the step of the stepped shaft, the protrusion being provided such that a gap exists between an upper surface of the top plate and the end surface of the step, the gap achieving a technical effect of enabling the diameter-adjusting member to be easily removed.

According to the utility model discloses an adsorption column supporting component for plasmid extraction, preferably, diameter adjusting part has a plurality ofly, i.e., in addition to a diameter adjusting part, still include at least one spare diameter adjusting part that has circular opening, wherein the overall dimensions of each spare diameter adjusting part all is the same with diameter adjusting part, the diameter of the circular opening of each spare diameter adjusting part all is less than the diameter of the circular opening of roof, the diameter of the circular opening of each spare diameter adjusting part all is different with the diameter of the circular opening of diameter adjusting part; each of the spare diameter adjusters is capable of replacing the diameter adjuster in situ to form different combinations. That is, the diameter of the opening of each of the diameter adjusting member and the spare diameter adjusting member corresponds to the diameter of the engagement portion of the adsorption column of one variety, and the circular opening of the top plate corresponds to the engagement portion of the adsorption column having the largest diameter. When in use, the technical effect of supporting adsorption columns with different diameters can be realized by replacing the diameter adjusting piece with a different standby diameter adjusting piece or by not using the diameter adjusting piece. More preferably, the vertical plate is connected to the bottom plate at one end portion and connected to the top plate at the other end portion. Thus, the whole adsorption column support assembly is substantially in the shape of a frame with one open surface.

The utility model discloses an adsorption column supporting component can adopt materials such as plastics, metal to make to adopt the machine-shaping method preparation commonly used. Specifically, the plastic material may be ABS, nylon, polyurethane, etc., and the metal material may be stainless steel, aluminum, copper, etc. If a plastic material is used, the common processing methods are injection molding, extrusion molding, and the like. If a metal material is used, the common machining method is machining, casting, stamping, bending, or the like. Preferably, the adsorption column support frame is made of stainless steel. The stainless steel, such as 304 stainless steel, has the advantages of less surface dead corners, high rigidity, and convenient sterilization and cleaning, and can satisfy GMP (good manufacturing Practice of Medical products) standards, i.e., drug manufacturing quality management standards, and class A operating environment standards, required for use in biosafety cabinets.

In the present invention, the "vertical plate" should be understood in a broad sense. Specifically, the vertical plate refers to any form of member capable of functioning as a support for the top and bottom plates. The shape of the riser includes but is not limited to: columnar, plate-like, or block-like (the transverse cross-section may be rectangular, circular, etc.).

It will be appreciated that the supported sorption column is a common device in the art for sorption operations. A typical adsorption column is substantially in the form of a vertically arranged cylinder, the top surface of which is open for the addition of a solution, and the bottom surface of which is provided with a weep section for the outflow of the solution. Wherein, the adsorption column is provided with the block department of easy block along the axial, the adsorption column can be fixed in circular opening. It should also be understood that the solution collection container described above refers to any form of container capable of collecting the solution flowing out of the adsorption column. For example, the solution collection container may be an open-top flat box, tray, or other form of container, etc.

Under the simplest condition, the utility model discloses an adsorption column supporting component only has a diameter regulating part to the roof of support frame does not have straight flute and ascending bending, and this kind of configuration simple structure, easily processing to can realize the support to the adsorption column of two kinds of diameters. That is, the adsorption column of one diameter is supported with the diameter adjuster being used, and the adsorption column of the other diameter is supported without the diameter adjuster being used. However, if the support stability of the adsorption column support assembly is to be enhanced and the support of more kinds of adsorption columns is to be realized at the same time, the two preferred embodiments provided by the present invention can be considered.

The utility model discloses a support frame roof of first preferred embodiment has straight flute and ascending bending, the diameter adjustment piece is flaky form, the line of buckling with the edge of diameter adjustment piece is tangent. This tangential arrangement defines the position of the diameter adjustment member on the top plate, thereby achieving the technical effect of enhancing the stability of the support. In addition, the diameter adjusting device further comprises a plurality of spare diameter adjusting pieces, and each spare diameter adjusting piece can replace the diameter adjusting piece in situ, so that support for more diameter varieties is realized. If the stability of the support is to be increased in only one step, a second preferred embodiment of the invention may be considered.

The utility model discloses a diameter adjustment spare of second preferred embodiment is the form of step shaft in vertical cross-section, the terminal surface department of the ladder of step shaft is provided with protrudingly, the diameter adjustment spare part passes the circular opening of roof, thereby will the diameter adjustment spare is fixed on the roof. Since the diameter adjustment member of this construction is fixed in position relative to the top plate during use, the stability of the support is further enhanced.

When the device is used, a proper component combination is selected, then the adsorption column is supported to a preset position, and then the solution collecting container is arranged below the adsorption column. Thereafter, an operation of adding the solution to be treated to an adsorption column, or the like may be performed.

The present invention will be described in detail with reference to the accompanying drawings, wherein the adsorbing column supporting assembly in the drawings is made of 304 stainless steel as an example material, and is manufactured by machining, casting, stamping, bending, etc. It should be understood that throughout the various drawings, identical components or identical features are denoted by the same reference numerals.

Figure 1 shows a perspective view of one embodiment of a sorption column support assembly, generally designated by the reference numeral 10. The adsorption column support assembly 10 includes a support frame 100 and a diameter adjusting member 200. The supporting frame 100 includes a top plate 101, a bottom plate 102 and a vertical plate 103. The top plate 101 and the bottom plate 102 are parallel to each other and horizontally disposed, the vertical plate 103 is perpendicular to the top plate 101 and the bottom plate 102, the top plate 101 and the bottom plate 102 are fixed on the same side of the vertical plate 103, the vertical plate 103 is connected to the bottom plate 102 at one end portion and connected to the top plate 101 at the other end portion, so that the whole adsorption column support assembly 10 is substantially in the shape of a frame with one open side. Fig. 2 shows a cross-sectional view of a front view of one embodiment of the sorption column support assembly shown in fig. 1, wherein the sorption column support assembly is cut along a vertical plane through a centerline of the sorption column support assembly. As shown in fig. 2, an upward bend is provided on the top plate 101, and the bend divides the top plate 101 into a horizontal portion and a bent portion, wherein an included angle α between the horizontal portion and the bent portion is 150 degrees. And, the diameter-adjusting piece 200 is superposed on the top plate 101, the circular opening 201 of the diameter-adjusting piece 200 is concentric with the circular opening 101A of the top plate 101, and the diameter of the circular opening 201 of the diameter-adjusting piece 200 is smaller than the diameter of the circular opening 101A of the top plate 101. Figure 3 shows a top view of one embodiment of the sorption column support assembly shown in figure 1. As shown in fig. 3, the top plate 101 is provided with a straight groove 101B extending from the edge of the top plate 101 to the center of the circular opening 101A to the circular opening 101A, and the bent line 101C perpendicularly intersects the straight groove 101B. It can also be seen from figure 3 that in this embodiment the diameter-adjusting member 200 is substantially in the form of a cylindrical flat piece with four arcuate cutouts, the circular opening 201 of the diameter-adjusting member 200 being located at the centre of the cylindrical flat piece, and in the predetermined position the edge of the diameter-adjusting member 200 is tangential to the bend line 101C of the bend. It should be understood that the diameter-adjusting member 200, which is in the form of a plate in practical use, may also have other cross-sectional forms.

Figure 4 shows a reference view of a state of use of one embodiment of the sorption column support assembly shown in figure 1. As shown in fig. 4, the adsorption column 20 is supported on the adsorption column support assembly 10 in a use state, and a solution collection container 30 for receiving a solution is disposed below the adsorption column 20. Because of the spacing effect that buckles produced, the position of adsorption column 20 is stable and is difficult for rocking. It should be understood that, in practical applications, in order to ensure the accurate positioning of the solution collecting container 30, a separate positioning member may be provided, or the positioning member may be integrated with the bottom plate of the adsorption column support assembly 10, so as to achieve the effective positioning of the solution collecting container 30.

Figure 5 shows a perspective view of another embodiment of a sorption column support assembly, generally designated by the reference numeral 10'. Wherein, the adsorption column support assembly 10 ' comprises a support frame 100 ' and a diameter adjusting member 200 '. Wherein the support frame 100 'comprises a top plate 101', a bottom plate 102 'and a vertical plate 103'. The top plate 101 'and the bottom plate 102' are parallel to each other and horizontally arranged, the vertical plate 103 'is perpendicular to the top plate 101' and the bottom plate 102 ', the top plate 101' and the bottom plate 102 'are fixed on the same side of the vertical plate 103', and the vertical plate 103 'is connected with the bottom plate 102' at one end and the top plate 101 'at the other end, so that the whole adsorption column support assembly 10' is substantially in the shape of a frame with one open surface. Figure 6 shows a cross-sectional view of a front view of another embodiment of the sorption column support assembly shown in figure 5, wherein the sorption column support assembly is cut along a vertical plane through the centre line of the sorption column support assembly. As shown in fig. 6, the diameter-adjusting piece 200 'is stacked on the top plate 101', and the circular opening 201 'of the diameter-adjusting piece 200' is concentric with the circular opening 101 'a of the top plate 101', and the diameter of the circular opening 201 'of the diameter-adjusting piece 200' is smaller than the diameter of the circular opening 101 'a of the top plate 101'. FIG. 7 is a partial view of FIG. 6, particularly illustrating details of the connection of the diameter adjuster to the top plate. As shown in fig. 7, the diameter adjuster 200 ' is in the form of a stepped shaft in vertical section, the circular opening 201 ' of the diameter adjuster 200 ' is concentric with the stepped shaft, the stepped shaft includes a first section 202 ' and a second section 203 ', the first section 202 ' has an outer diameter equal to the diameter of the circular opening 101 ' a of the top plate 101 ', the second section 203 ' has an outer diameter greater than the diameter of the circular opening 101 ' a of the top plate 101 ', and the first section 202 ' of the diameter adjuster 200 ' passes through the circular opening 101 ' a of the top plate 101 ', thereby fixing the diameter adjuster 200 ' on the top plate 101 '. As can also be seen from fig. 7, a protrusion 204 ' is provided at an end surface of the step of the stepped shaft, the protrusion 204 ' being provided such that a gap exists between the upper surface of the top plate 101 ' and the end surface of the step. Figure 8 shows a top view of another embodiment of the sorption column support assembly shown in figure 5. As shown in fig. 8, the top plate 101 ' is provided with a straight groove 101 ' B extending from the edge of the top plate 101 ' to the center of the circular opening 101 ' a to the circular opening 101 ' a.

Fig. 9 is a reference view showing a state of use of another embodiment of the adsorption column support assembly shown in fig. 5. As shown in fig. 9, the adsorption column 20 is supported on the adsorption column support assembly 10' in a use state, and a solution collection container 30 for receiving a solution is disposed below the adsorption column 20. Because under the user state, diameter adjusting part and roof fixed connection, the stable difficult rock in position of adsorption column 20. It should be understood that, in practical applications, in order to ensure the accurate positioning of the solution collection container 30, a separate positioning member may be provided, or the positioning member may be integrated with the bottom plate of the adsorption column support assembly 10', so as to achieve the effective positioning of the solution collection container 30.

It will be appreciated that although the figures above show a combination of diameter adjustment members and a support frame, in actual use the support frame may be used alone to support a larger diameter column or the diameter adjustment members may be replaced by spare diameter adjustment members to obtain a combination that provides a different support diameter.

Claims

1. An adsorption column support assembly for plasmid extraction, comprising:

a support frame, and

a diameter adjustment member;

2. The adsorption column support assembly for plasmid extraction according to claim 1, wherein the opening of the diameter adjusting member and the opening of the top plate are in the form of circular openings, wherein a position limiting mechanism for limiting the position of the diameter adjusting member relative to the top plate is provided between the diameter adjusting member and the top plate.

3. The adsorption column support assembly for plasmid extraction according to claim 2, wherein the top plate is provided with a straight groove extending from the edge of the top plate to the center of the circular opening of the top plate, and the center line of the straight groove extends through the center of the circular opening of the top plate.

4. The adsorption column support assembly for plasmid extraction as defined in claim 2, wherein the diameter adjusting member is in the form of a sheet, and the top plate is further provided with an upward bend, and the bend line of the upward bend is tangential to the edge of the diameter adjusting member, thereby forming the limiting mechanism.

5. The support assembly of claim 4, wherein the angle between the horizontal portion and the bent portion of the top plate is in the range of 120 to 170 degrees.

6. The adsorption column support assembly for plasmid extraction according to claim 2, wherein the diameter adjuster is in the form of a stepped shaft in vertical section, the circular opening of the diameter adjuster is concentric with the stepped shaft, the stepped shaft comprises a first section and a second section, the first section has an outer diameter smaller than or equal to the diameter of the circular opening of the top plate, the second section has an outer diameter larger than the diameter of the circular opening of the top plate, and the first section of the diameter adjuster passes through the circular opening of the top plate to constitute the position limiting mechanism.

7. The adsorption column support assembly for plasmid extraction of claim 6, wherein a protrusion is provided at an end face of the step of the stepped shaft.

8. The adsorption column support assembly for plasmid extraction according to any one of claims 2 to 7, further comprising at least one spare diameter adjusting member having a circular opening, wherein each spare diameter adjusting member has the same overall dimension as the diameter adjusting member, the diameter of the circular opening of each spare diameter adjusting member is smaller than that of the circular opening of the top plate, and the diameter of the circular opening of each spare diameter adjusting member is different from that of the circular opening of the diameter adjusting member; each of the spare diameter adjusters is capable of replacing the diameter adjuster in situ to form different combinations.

9. The support assembly of claim 8, wherein the vertical plate is connected to the bottom plate at one end and to the top plate at the other end.