CN217336954U - Snake venom collection system - Google Patents
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- CN217336954U CN217336954U CN202221349656.0U CN202221349656U CN217336954U CN 217336954 U CN217336954 U CN 217336954U CN 202221349656 U CN202221349656 U CN 202221349656U CN 217336954 U CN217336954 U CN 217336954U
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Abstract
A snake venom collection device comprises a refrigerating device, a storage container, a vacuumizing device and a venom extractor which penetrates through the top of the refrigerating device, extends into the refrigerating device and is used for extracting venom, wherein an outer side pipe communicated with the vacuumizing device and an embedded main pipe communicated with the storage container are arranged at the bottom of the venom extractor, at least more than 2 layers of sterile flexible membranes are arranged at the top of the venom extractor, and the storage container is placed in the refrigerating device and is in sealing connection with the bottom of the venom extractor. The snake venom collection device can effectively retain active ingredients in snake venom, reduce the residual quantity of venom automatically sprayed by the snake on the inner wall of the venom extractor, improve the efficiency and yield of snake venom collection, and has convenient use and simple structure.
Description
Technical Field
The utility model relates to a biological product collection system especially relates to a snake venom collection system.
Background
Snake venom is a liquid secreted from the venom gland of poisonous snake, and contains mainly toxic protein and active component, accounting for 90-95% of dry weight, and more than twenty kinds of enzymes and toxins. In addition, the composition also contains small molecular peptides, amino acids, carbohydrates, lipids, nucleosides, biogenic amines, metal ions and the like. The snake venom components are very complex, and the toxic, pharmacological and toxicological effects of different snake venom are respectively characterized, so the snake venom has good medicinal value in the traditional Chinese medicine and pharmacy. The snake venom collection method comprises two modes of dead collection and live collection. The dead harvest is a special destructive method for cutting off the venom gland from the head of a snake after the head of the snake is cut off by a processing unit or the snake in the posterior sulcus and a sea snake with less toxic production are anesthetized and killed, and the killing virus is taken out to kill species, and is only occasionally used under special conditions. The "live mining" method is generally used to preserve snake resources. Common "live mining" methods include: electrical stimulation poison collection method, dish-biting poison collection method, membrane-biting poison collection method and the like. For example, the snake venom extracting and storing device disclosed in CN209105930U and the novel snake venom extracting and storing device disclosed in CN203913008U adopt a membrane-biting venom collecting method, which has low venom collecting efficiency, low impurity removal rate in snake venom and uncontrollable snake venom storage temperature, thus easily affecting the activity of snake venom and reducing the economic value thereof.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is: overcomes the defects of the prior art, and provides a snake venom collection device which has simple structure, convenient use, high venom collection efficiency and can effectively retain the activity of snake venom.
The utility model provides a technical scheme that its technical problem adopted is:
a snake venom collection device comprises a refrigerating device, a storage container, a vacuumizing device and a venom extractor which penetrates through the top of the refrigerating device, extends into the refrigerating device and is used for extracting venom, wherein an outer side pipe communicated with the vacuumizing device and an embedded main pipe communicated with the storage container are arranged at the bottom of the venom extractor, at least more than 2 layers of sterile flexible membranes are arranged at the top of the venom extractor, and the storage container is placed in the refrigerating device and is in sealing connection with the bottom of the venom extractor.
In an exemplary embodiment, a filter core is disposed in the middle of the poison extractor. Preferably, a microporous filter membrane is arranged on the filter element. The filter element in the venom extractor can filter the venom original liquid left by the sterile flexible membrane to remove impurities therein, particularly the filter element with the microporous filter membrane, so that the impurities in the venom original liquid can be filtered, and the venom extractor without the filter element or the microporous filter membrane filter element can effectively maintain the negative pressure environment in the space enclosed by the storage container and the bottom of the venom extractor (particularly under the condition that the sterile flexible membrane is torn by snake teeth after being used for many times), so that the venom original liquid is promoted to rapidly enter the storage container through the venom extractor.
In an exemplary embodiment, the poison extractor is any one of a sand core suction filter funnel, a glass plate suction filter funnel or a suction filter funnel with a cooling jacket, and the upper part of the poison extractor is a cylindrical cylinder or a conical cylinder.
The number of the sterile flexible membranes is 2-20, and the sterile flexible membranes are in direct proportion to the length of the teeth and the biting force of the snake, for example, when the snake venom of the agkistrodon acutus is collected, 14-20 sterile flexible membranes (3M sterile flexible membranes) are adopted, and when the snake venom of the agkistrodon acutus is collected, 5-7 sterile flexible membranes are adopted. The multi-layer sterile flexible membrane can stimulate the snake to actively spray venom and can also carry out primary filtration on the snake venom to reduce the infiltration of impurities.
In an exemplary embodiment, the sterile flexible membrane is secured to the top of the extractor by a gripping member.
The clamping part is a plastic hose clamp or a clamping ring with a tightening bolt.
The vacuum pumping device is an oil-free diaphragm vacuum pump.
In one exemplary embodiment, the refrigeration device is a digital display temperature control vehicle-mounted direct current refrigerator, and the common refrigeration temperature is-4 ℃. The storage container is preferably a ground-top conical flask, and a beaker, a flask, a test tube, or the like may be used. The cold storage device is internally provided with a supporting plate for supporting the storage container and is well connected with the storage container and the bottom of the poison extractor. The top of the refrigerating device is provided with a through hole for the poison extractor to penetrate through, and the through hole is provided with a sealing plug, so that the refrigerating device is ensured to provide a stable refrigerating environment.
In an exemplary embodiment, the snake venom collection device further comprises a moving trolley, and a frame body for placing the vacuum pump and the refrigerating device is arranged on the moving trolley, so that the snake venom collection device can be conveniently and rapidly moved for use.
The utility model relates to a snake venom collection device is used for gathering snake venom method, including following step:
1) preparing a snake venom collection device: determining the number of layers of the sterile flexible membrane according to the tooth length and the occlusal force of the snake type of the collected snake venom; then the snake venom collection device is assembled, namely the storage container is placed into a refrigerating device, the venom extractor is hermetically connected with the top of the storage container, then an outer side pipe of the venom extractor is connected with a vacuumizing device through a hose, a microporous filter membrane is laid in the venom extractor, then a plurality of layers of sterile flexible membranes are laid on the top of the venom extractor, and the venom extractor is fixed through a clamping part; starting the refrigerating device, and adjusting the temperature of the refrigerating device to a specified refrigerating temperature;
2) collecting venom: starting a vacuumizing device, contacting the head of the snake with a sterile flexible membrane at the top of the venom extractor to lure the snake to actively open the mouth and bite the sterile flexible membrane, inserting the teeth of the snake into the membrane and automatically spraying out venom, wherein the venom quickly passes through a microporous filter membrane under the action of the vacuumizing device and flows into a storage container along an embedded main pipe of the venom extractor, and the venom quickly passes through the microporous filter membrane under the suction action of negative pressure formed by the vacuumizing device and flows into the storage container along the embedded main pipe of the venom extractor;
3) and (5) putting the snake with the collected venom back to the feeding box.
The pore diameter of the microporous filter membrane is 20-100 mu m, for example, mucus of Agkistrodon acutus is thinner, the microporous filter membrane with the pore diameter of 20 mu m is adopted, mucus of cobra is thicker, and the microporous filter membrane with the pore diameter of 100 mu m is adopted.
When in use, the vacuum pumping device maintains the vacuum degree in the storage container and the venom extractor between-0.03 MPa and-0.05 MPa.
An enzyme protective agent for stabilizing the activity of snake venom, preferably a thrombin-like enzyme protective agent, is added into the storage container. The thrombin-like enzyme protective agent is one or more selected from glycine and other amino acids, dextran, trehalose, human serum albumin and hydrolyzed gelatin. The enzyme protective agent is stored at 2-8 ℃, and is added into a storage container when in use.
The utility model relates to a snake venom collection system's beneficial effect:
the utility model discloses snake venom collection system adopts intercommunication evacuating device's the ware of getting venom, utilizes evacuating device to make the inside little negative pressure that forms of storage container and sampler for the automatic venom that sprays of snake flows into storage container from getting the venom ware, is favorable to keeping of active ingredient in the snake venom, and reduces the automatic venom that sprays of snake and at the volume of remaining of getting the venom ware inner wall, improves the efficiency and the yield that the snake venom was gathered.
And secondly, a microporous filter membrane is laid on a filter core in the venom extractor of the snake venom collection device to re-filter the snake venom, so that the impurity content of the snake venom entering the storage container is reduced, and the purity of the snake venom is improved.
In addition, snake venom collection system, convenient to use, simple structure adopts the on-vehicle direct current refrigerator of digital display control by temperature change to provide a invariable environment for the storage of snake venom, avoids the deactivation of snake venom.
Drawings
FIG. 1 is a schematic view showing a snake venom collection device according to example 1;
FIG. 2 is a perspective view of a venom extractor of a snake venom collection device according to example 1;
FIG. 3 is a schematic view showing a snake venom collection device according to example 2;
FIG. 4 is a schematic view of the snake venom collection device of example 3;
fig. 5-is the structure diagram of the venom extractor of the snake venom collection device in embodiment 3.
Wherein: 1. the device comprises a sterile flexible membrane, 2, a clamping component, 3, a poison taking device, 31, a filter element, 32, an outer pipe, 33, an embedded main pipe, 34, a ground joint, 35, a cooling jacket, 4, a conical bottle with a ground opening, 5, a refrigerating device, 51, a supporting plate, 52, a sealing plug, 6, a vacuumizing device, 7 and a moving trolley.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
Example 1
Referring to fig. 1-2, the snake venom collection device of the present embodiment includes a refrigeration device 5, a storage container, a vacuum extractor 6, and a venom extractor 3 penetrating the top of the refrigeration device 5 and extending into the interior of the refrigeration device for extracting venom, wherein an outer tube 32 communicating with the vacuum extractor 6 and an embedded main tube 33 communicating with the storage container are disposed at the bottom of the venom extractor 3, the top of the venom extractor 3 is provided with more than 2 layers of sterile flexible membranes 1, and the storage container is disposed in the refrigeration device 5 and hermetically connected to the bottom of the venom extractor 3.
The middle part of the poison taking device 3 is provided with a filter core 31, and a 20 mu m microporous filter membrane is arranged on the filter core 31.
The poison taking device 3 is a sand core suction filtration funnel, and the upper part of the poison taking device 3 is a cylindrical barrel.
When the snake venom collection device is used for collecting snake venom of the agkistrodon acutus, as the teeth of the agkistrodon acutus are longer and basically 8-16 mm, the number of the sterile flexible membrane 1 is 14-20, and is in direct proportion to the length of the teeth of the agkistrodon acutus and the occlusal force, the thickness of the multi-layer sterile flexible membrane 1 is slightly smaller than the length of the teeth of the agkistrodon acutus by 1-2 mm, and further the multi-layer sterile flexible membrane 1 can stimulate the agkistrodon acutus to actively spray venom and can perform primary filtration on the snake venom, so that the infiltration of impurities is reduced.
When the snake venom collection device of this embodiment is used for the snake venom collection of cobra, because the tooth of cobra is shorter, is basically at 6 ~ 9 millimeters, the number of piles of aseptic flexible membrane 1 is 5 ~ 7 layers, and is directly proportional with the tooth length of snake, occlusal force, makes the thickness of aseptic flexible membrane 1 of multilayer slightly littleer 0.5 ~ 1mm than the tooth length of cobra, and then can stimulate the snake through aseptic flexible membrane 1 of multilayer and initiatively spray the venom, can also carry out primary filtration to the snake venom, reduces the infiltration of impurity.
When the snake venom collection device is used for collecting snake venom of the bungarus multicinctus, because the teeth of the bungarus multicinctus are the shortest in tooth length of the bungarus multicinctus and basically 2-4 mm, the number of layers of the sterile flexible membrane 1 is 2-4, and is in direct proportion to the tooth length and the biting force of the bungarus multicinctus, the thickness of the multi-layer sterile flexible membrane 1 is slightly smaller than the tooth length of the cobra by 0.5-1 mm, and then the bungarus multicinctus can stimulate the bungarus multicinctus to actively spray venom through the multi-layer sterile flexible membrane 1, and can also carry out primary filtration on the snake venom, so that the permeation of impurities is reduced.
The sterile flexible membrane 1 is fixed on the top of the venom extractor 3 through a clamping part 2, and preferably, the clamping part 2 is a clamping ring with a tightening bolt. When the device is used, the sterile flexible membrane 1 is covered on the poison extractor 3, and then the sterile flexible membrane 1 is fixed on the poison extractor 3 by the clamping parts 2 such as a hoop or a rubber band, so that the fixation is firm, and the sterile flexible membrane 1 is prevented from being loosened in the poison extracting process.
The vacuum pumping device 6 is an oilless diaphragm vacuum pump, and can avoid the phenomenon of polluting snake venom samples when an oil pump type/water pump type vacuum pump is adopted for suck back.
The refrigerating device 5 is a digital display temperature control vehicle-mounted direct current refrigerator, and the common refrigerating temperature is-4 ℃. The storage container is preferably a conical bottle 4 with a grinding opening, the grinding opening part of the conical bottle 4 with the grinding opening is clamped with the grinding opening joint 34 of the virus extractor 3, the sealing performance is good, and the sealing condition required by the vacuumizing device 6 for vacuumizing is ensured. The refrigerating device 5 is provided with a support plate 51 for supporting the storage container, and is well connected with the storage container and the bottom of the venom extractor 3. The top of the refrigerating device 5 is provided with a through hole for the venom extractor 3 to penetrate through, and the through hole is provided with a sealing plug 52, so that the refrigerating device 5 is ensured to provide a stable refrigerating environment. The refrigerating device 5 may also adopt other devices capable of refrigerating to provide a low temperature environment, such as a micro-freezer, a micro-refrigerator, a micro-freezer, or other containers filled with a refrigerant, which is ice, other low-temperature or heat-absorbing substances.
It should be noted that the poison taking device 3 of the present invention is a glass plate suction filtration funnel, and the top thereof can also be a cone-shaped cylinder.
The use method of the snake venom collection device comprises the following steps:
1) preparing a snake venom collection device: determining the number of layers of the sterile flexible membrane 1 according to the tooth length and the occlusal force of the snake species for collecting snake venom, laying 5-7 layers of the sterile flexible membrane 1 when taking cobra venom, laying 14-20 layers of the sterile flexible membrane 1 when taking agkistrodon venom, and laying 2-4 layers of the sterile flexible membrane 1 when taking agkistrodon venom; then the snake venom collection device is assembled, namely the storage container is placed in a refrigerating device 5, the venom extractor 3 is hermetically connected with the top of the storage container, then an outer side pipe 32 of the venom extractor 3 is connected with a vacuumizing device 6 through a hose, a microporous filter membrane is laid in the venom extractor 3, then a plurality of layers of sterile flexible membranes 1 are laid on the top of the venom extractor 3 and are fixed through a clamping part 2; starting the refrigerating device 5, and adjusting the temperature of the refrigerating device 5 to a specified refrigerating temperature;
2) collecting venom: starting the vacuum-pumping device 6, contacting the snake head of the snake with the sterile flexible membrane 1 at the top of the venom extractor 3 to induce the snake to actively open the mouth and bite the sterile flexible membrane 1, inserting the snake teeth into the membrane and automatically spraying out venom;
3) and (5) putting the snake with the collected venom back to the feeding box.
The pore diameter of the microporous filter membrane is 20-100 mu m, for example, mucus of Agkistrodon acutus is thinner, the microporous filter membrane with the pore diameter of 20 mu m is adopted, mucus of cobra is thicker, and the microporous filter membrane with the pore diameter of 100 mu m is adopted.
When in use, the vacuum-pumping device 6 maintains the vacuum degree in the storage container and the poison extractor 3 between-0.03 MPa and-0.05 MPa.
Alternatively, a method of using a snake venom collection device, comprising the steps of:
1) preparing a snake venom collection device: determining the number of layers of the sterile flexible membrane 1 according to the tooth length and the occlusal force of the snake species for collecting snake venom, laying 5-7 layers of the sterile flexible membrane 1 when taking cobra venom, laying 14-20 layers of the sterile flexible membrane 1 when taking agkistrodon venom, and laying 2-4 layers of the sterile flexible membrane 1 when taking agkistrodon venom; then the snake venom collection device is assembled, namely the storage container is placed in a refrigerating device 5, the venom extractor 3 is hermetically connected with the top of the storage container, then an outer side pipe 32 of the venom extractor 3 is connected with a vacuumizing device 6 through a hose, a microporous filter membrane is laid in the venom extractor 3, then a plurality of layers of sterile flexible membranes 1 are laid on the top of the venom extractor 3 and are fixed through a clamping part 2; starting the refrigerating device 5, and adjusting the temperature of the refrigerating device 5 to a specified refrigerating temperature;
2) collecting venom: adding an enzyme protective agent for stabilizing snake venom activity into a storage container, such as adding 1% glycine (the enzyme protective agent is stored at 2-8 deg.C, and added into the storage container when in use); starting the vacuum-pumping device 6, contacting the snake head of the snake with the sterile flexible membrane 1 at the top of the venom extractor 3 to attract the snake to bite the sterile flexible membrane 1 by actively opening mouth, inserting the snake teeth into the membrane and automatically spraying out venom, filtering the venom through a microporous filter membrane quickly under the negative pressure suction effect formed by the vacuum-pumping device 6, and flowing into the storage container 4 along the embedded main pipe of the venom extractor 3;
3) and (5) putting the snake with the collected venom back to the feeding box.
The pore diameter of the microporous filter membrane is 20-100 mu m, for example, mucus of Agkistrodon acutus is thinner, the microporous filter membrane with the pore diameter of 20 mu m is adopted, mucus of cobra is thicker, and the microporous filter membrane with the pore diameter of 100 mu m is adopted.
When in use, the vacuum-pumping device 6 maintains the vacuum degree in the storage container and the poison extractor 3 between-0.03 MPa and-0.05 MPa.
Example 2
Referring to fig. 3, the snake venom collection device of this embodiment has the following differences compared to embodiment 1:
the snake venom collection device further comprises a movable trolley 7, and a frame body used for placing a vacuumizing device 6 (such as a vacuum pump) and a refrigerating device 5 is arranged on the movable trolley 7, so that the snake venom collection device can be conveniently and quickly moved for use. The mobile cart 7 may be a trolley or a small car.
The using method is the same as that described in the embodiment 1, and the differences exist: the snake venom collection device is rapidly moved by the movable trolley 7.
Example 3
Referring to fig. 4 and 5, the snake venom collection device of this embodiment has the following differences compared to embodiment 1:
the poison taking device 3 is a glass plate suction filtration funnel with a cooling jacket 35, and the upper part of the poison taking device 3 is a cylindrical barrel. The cooling jacket of the venom extractor 3 is communicated with a cooling water circulator, and the temperature of cooling water in the venom extractor is preferably 2-8 ℃, so that the effective active ingredients in the venom stock solution flowing into the venom extractor 3 can be better retained.
The using method is different from that of the embodiment 1 in the following points: 2-8 ℃ cooling water is introduced into the cooling jacket 35 of the venom extractor 3 through a cooling water circulator, so that the temperature of the side wall of the venom extractor and the temperature in the inner cavity of the venom extractor are lower than room temperature, preferably 2-8 ℃, and further the active ingredients of the venom stock solution are prevented from being inactivated due to the environmental temperature in the collection process, and the content and the activity of the active ingredients of the venom are improved.
The utility model relates to a snake venom collection device, when using, according to the snake kind difference of snake venom source, combine the tooth length and the occlusal force size of snake, adjust and get the number of piles of 3 top aseptic flexible membranes 1 of venom extractor, for example, when getting the snake venom of black man's babysnade, the number of piles based on black man's babysnade is generally 3-6 millimeters, and 5-8 layers can be selected to aseptic flexible membrane 1; the above technical features can be understood and implemented by those skilled in the art through the text description, and thus, detailed description is not provided for further embodiments.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may include, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.
Claims (9)
1. The snake venom collection device is characterized by comprising a refrigerating device (5), a storage container, a vacuumizing device (6) and a venom extractor (3) which penetrates through the top of the refrigerating device (5) and extends into the refrigerating device and is used for extracting venom, wherein an outer side pipe (32) communicated with the vacuumizing device (6) and an embedded main pipe (33) communicated with the storage container are arranged at the bottom of the venom extractor (3), at least more than 2 layers of sterile flexible membranes (1) are arranged at the top of the venom extractor (3), and the storage container is placed in the refrigerating device (5) and is hermetically connected with the bottom of the venom extractor (3).
2. A snake venom collection device according to claim 1, wherein a filter element (31) is provided in the middle of the venom extractor (3), and a microporous membrane is placed on the filter element (31).
3. The snake venom collection device according to claim 2, wherein the venom extractor (3) is any one of a sand core suction filter funnel, a glass plate suction filter funnel or a suction filter funnel with a cooling jacket, and the upper part of the venom extractor (3) is a cylindrical barrel or a conical barrel.
4. A snake venom collection device according to claim 1 or 2, wherein the number of the sterile flexible membrane (1) is 2-20 and is proportional to the length of the snake teeth and the biting force.
5. A snake venom collection device according to claim 1 or 2, wherein the sterile flexible membrane (1) is fixed to the top of the venom extractor (3) by a clamping member (2).
6. A snake venom collection device according to claim 5, further comprising a mobile cart (7), wherein the mobile cart (7) is provided with a frame body for placing the vacuum extractor (6) and the refrigerating device (5).
7. A snake venom collection device according to claim 1 or 2, wherein the vacuum pumping device (6) is an oil-free diaphragm vacuum pump, and the refrigerating device (5) is a digital display temperature control vehicle-mounted DC refrigerator.
8. A snake venom collection device according to claim 1 or 2, wherein the cold storage device (5) is provided with a support plate (51) for supporting the storage container.
9. A snake venom collection device according to claim 1 or 2, wherein the top of the cold storage device (5) is provided with a through hole for the venom extractor (3) to pass through, and a sealing plug (52) is arranged at the through hole.
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| CN202221349656.0U CN217336954U (en) | 2022-05-31 | 2022-05-31 | Snake venom collection system |
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| CN202221349656.0U CN217336954U (en) | 2022-05-31 | 2022-05-31 | Snake venom collection system |
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