CN217304909U - Portable biological detection system - Google Patents

Abstract

The utility model provides a portable biological detection system, which comprises a base, a sample placing layer, a light source, a photosensitive layer and a control unit. The sample placing layer is arranged on the base and comprises at least one detection area, and the at least one detection area is used for placing a bearing sheet containing biological samples on the at least one detection area. The light source is arranged in the base and emits light to the at least one detection area. The photosensitive layer is located above the sample placement layer and includes at least one sensor aligned with the at least one detection zone. The control unit is electrically connected with the light source and the at least one sensor. Therefore, the utility model discloses a portable biological detection system can carry out whole transformation to optical path through structural configuration to reduce optical assembly quantity, reach efficiency such as reduce the volume, weight reduction, reduce cost.

Description

Portable biological detection system

Technical Field

The utility model relates to a biological detection system, especially a portable biological detection system who carries out whole transformation to optical path.

Background

The prior art biological detection system comprises a sample placing layer, a light source, a first optical filter, a spectroscope, an objective lens, a second optical filter, a sensor and a control unit. The method of use of the prior art biological detection system comprises the steps of: placing a carrier sheet containing a biological sample on the detection zone of the sample placement layer; the light source projects light rays, and the light rays pass through the first optical filter to select a required radiation waveband; the filtered light is reflected to the objective lens through the spectroscope; the objective lens focuses light on the biological sample on the bearing sheet, and the biological sample or the biological molecules to be detected carried by the biological sample are excited by the light to generate chemiluminescence or photoluminescence (such as fluorescence); the chemiluminescence or photoluminescence sequentially passes through the objective lens, the spectroscope and the second optical filter; the chemiluminescence or photoluminescence is transmitted through a second optical filter to select a required radiation waveband; the sensor receives the filtered chemiluminescence or photoluminescence; and the control unit obtains a detection result according to the chemiluminescence signal or the photoluminescence signal.

However, the prior art biological detection system has many optical components, and has the problems of large volume, heavy weight, high cost, and the like. Therefore, the biological detection system of the prior art is only suitable for being placed in a laboratory for use and is not suitable for being moved in different places. For users with stable detection requirements, the medical institution is frequently carried out detection in a round trip manner, and the round trip time and the cost of traffic are increased.

SUMMERY OF THE UTILITY MODEL

A primary object of the present invention is to provide a portable biological detection system, which can be configured structurally to perform an overall modification of an optical path to reduce the number of optical components.

In order to achieve the above objective, the present invention provides a portable biological detection system, which includes a base, a sample placing layer, a light source, a photosensitive layer and a control unit. The sample placing layer is arranged on the base and comprises at least one detection area, and the at least one detection area is used for placing a bearing sheet containing biological samples on the at least one detection area. The light source is arranged in the base and emits light to the at least one detection area. The photosensitive layer is located above the sample placement layer and includes at least one sensor aligned with the at least one detection zone. The control unit is electrically connected with the light source and the at least one sensor.

In some embodiments, the sample placement layer includes a plurality of detection areas, the photosensitive layer includes a cover, a first turntable, and a plurality of sensors, the cover is located above the sample placement layer, the first turntable is disposed on the cover and electrically connected to the control unit and capable of rotating relative to the cover, and the plurality of sensors are disposed on the first turntable at intervals and aligned with the plurality of detection areas respectively.

In some embodiments, the sample placement layer includes a fixing base and a second rotating disc, the fixing base is disposed on the base, the second rotating disc is disposed on the fixing base, the second rotating disc includes a plurality of detection regions and is electrically connected to the control unit and can rotate relative to the fixing base, a light transmission portion is disposed at the bottom of each detection region, the light source emits light to one of the light transmission portions, and the light passes through one of the light transmission portions and is projected onto one of the detection regions.

In some embodiments, the second turntable is capable of moving horizontally relative to the fixed base.

In some embodiments, the control unit turns off the at least one sensor when the control unit turns on the light source; and wherein the control unit activates the at least one sensor when the control unit turns off the light source.

In some embodiments, the control unit synchronously controls the period of activation and deactivation of the light source and the period of activation and deactivation of the sensor.

In some embodiments, the light source is coupled to one end of an optical fiber, the other end of the optical fiber being aligned with the at least one detection zone; when the light source emits light, the light of the light source passes through the optical fiber and is emitted from the other end of the optical fiber to at least one detection region.

In some embodiments, the photosensitive layer further includes at least one filter disposed on a side of the at least one sensor facing the at least one detection area.

In some embodiments, the photosensitive layer further includes at least one objective lens aligned with the at least one detection area and electrically connected to the control unit.

In some embodiments, the portable biological detection system further includes a display screen disposed on the base or the photosensitive layer and electrically connected to the control unit.

The utility model has the advantages that:

the utility model discloses a portable biological detection system can carry out whole transformation to optical path through structural configuration to reduce optical assembly quantity, reach efficiency such as reduce the volume, weight reduction, reduce cost.

Drawings

Fig. 1 is a perspective view of a portable biological detection system according to an embodiment of the present invention.

Fig. 2 is a perspective view of the first embodiment of the present invention with the cover opened.

Fig. 3 is a cross-sectional view of line a-a of fig. 1, showing a first light source arrangement of the present invention.

Fig. 4 is a cross-sectional view of line a-a of fig. 1, showing a second light source arrangement of the present invention.

Fig. 5 is a schematic structural diagram of the first embodiment of the present invention.

Fig. 6 is a perspective view of the second embodiment of the present invention with the cover opened.

Fig. 7 is a cross-sectional view of line B-B of fig. 6.

Fig. 8 is a plan view of a photosensitive layer according to a third embodiment of the present invention.

Fig. 9 is a schematic structural diagram of a third embodiment provided by the present invention.

Fig. 10 is a plan view of a photosensitive layer according to a fourth embodiment of the present invention.

Description of reference numerals:

10, a base; 20, a sample placing layer; 21, a fixed seat; 22, a second turntable; 221, a detection area; 222, a light transmission part; 30, a light source; 31: an optical fiber; 40,40A,40B,40C, a photosensitive layer; 41, a cover body; 42, a first turntable; 421, a containing groove; 43, a sensor; 44, an optical filter; 45, an objective lens; 50, a control unit; 60, displaying a screen; 70, a server; 100, carrying sheet.

Detailed Description

The embodiments of the present invention will be described in more detail with reference to the drawings and the reference numerals, so that those skilled in the art can read the description to implement the embodiments.

Fig. 1 is a perspective view of a portable biological detection system provided by an embodiment of the present invention, fig. 2 is a perspective view of the first embodiment of the present invention with a cover 41 lifted, fig. 3 is a sectional view of line a-a of fig. 1, showing a first light source setting mode of the present invention, fig. 4 is a sectional view of line a-a of fig. 1, showing a second light source setting mode of the present invention, and fig. 5 is a schematic structural view of the first embodiment of the present invention. As shown in fig. 1 to 5, the present invention provides a portable biological detection system, which includes a base 10, a sample placing layer 20, a light source 30, a photosensitive layer 40 and a control unit 50. The sample-placing layer 20 is disposed on the base 10 and includes at least one detection zone 221, and the at least one detection zone 221 is used for placing a carrier sheet 100 containing biological samples thereon. The light source 30 is disposed in the base 10 and emits a light to at least one detection area 221. The photosensitive layer 40 is positioned above the sample placement layer 20 and includes at least one sensor 43, the at least one sensor 43 being aligned with the at least one detection zone 221. The control unit 50 is electrically connected to the light source 30 and the at least one sensor 43.

How the portable biological detection system of the present invention detects a biological sample will be further described with reference to the drawings.

First, as shown in FIG. 2, a support sheet 100 containing a biological sample is placed on the detection zone 221. Then, as shown in fig. 3 to fig. 5, the control unit 50 activates the light source 30, the light source 30 emits light to the detection region 221, the biological sample on the carrier sheet 100 or the to-be-detected biomolecules carried by the biological sample generate chemiluminescence or photoluminescence (indicated by arrows in fig. 3 or fig. 4) under excitation of the light, and the sensor 43 receives the chemiluminescence or photoluminescence (e.g., fluorescence). Finally, the control unit 50 obtains a detection result according to the chemiluminescence signal or the photoluminescence signal.

As shown in FIG. 3, the first light source is arranged such that the light source 30 is coupled to one end of an optical fiber 31, and the other end of the optical fiber 31 is aligned with the detection region 221; when the light source 30 emits light, the light of the light source 30 passes through the optical fiber 31 and is emitted from the other end of the optical fiber 31 toward the detection region 221. This arrangement has the advantage that the light source 30 can be located anywhere inside the base 10, which helps to reduce the volume of the base 10.

As shown in FIG. 4, the second light source is disposed such that the light source 30 is aligned with the detection area 221; when the light source 30 emits light, the light of the light source 30 is directly emitted toward the detection region 221. This arrangement has the advantage of being less costly without the need for optical fibers 31.

Preferably, the light source 30 is a laser diode, the light emitted by the laser diode is laser light, the biological sample is a tissue slice, the biomolecule to be detected can be, for example, protein, peptide, antibody, nucleic acid, etc., and the biological sample or the biomolecule to be detected carried by the biological sample can be combined with a fluorescent marker, a reporter marker, or a chemiluminescent marker. The fluorescent label may be, but is not limited to, FITC (fluorescein isothiocyanate), HEX (6-carboxy-2 ', 4,4 ', 5 ', 7,7 ' -hexachlorofluorescein), FAM (6-carboxyfluorescein), TAMRA (N, N ' -tetramethyl-6-carboxyrhodamine), Cy3 (indodicarbocyanine 3), Cy5 (indodicarbocyanine 5), quantum dot (quantum dot), etc., and may be used in combination with a shielding dye (quincher dye).

In some embodiments, when the control unit 50 starts the light source 30, the light source 30 emits light to the detection region 221, the biological sample on the carrier sheet 100 or the biological molecules to be detected carried by the biological sample are excited by the light to generate chemiluminescence or photoluminescence, and the control unit 50 turns off the sensor 43 at the same time, so as to prevent the sensor 43 from being interfered by the light in the process of receiving the chemiluminescence or photoluminescence, thereby reducing the accuracy of the detection result. When the control unit 50 turns off the light source 30, the biological sample on the carrier sheet 100 or the biomolecules to be detected carried by the biological sample can still continuously generate chemiluminescence or photoluminescence, and meanwhile, the control unit 50 turns on the sensor 43, so that the sensor 43 is not interfered by light rays in the process of receiving the chemiluminescence or photoluminescence, and the accuracy of the detection result is further improved. In other words, the light source 30 emits light intermittently, and the sensor 43 receives chemiluminescence or photoluminescence intermittently, so as to ensure that the detection result has high accuracy and is not interfered by light.

In some embodiments, the control unit 50 controls the period of activation and deactivation of the light source 30 and the period of activation and deactivation of the sensor 43 synchronously. For example, the light source 30 is on for five seconds while the sensor 43 is off for five seconds, then the light source 30 is off for five seconds while the sensor 43 is on for five seconds. In other words, the biological sample or the biomolecules to be detected carried by the biological sample are excited for multiple times at a fixed period to generate chemiluminescence or photoluminescence, and the chemiluminescence or photoluminescence is received for multiple times at the fixed period, so that a chemiluminescence signal or a photoluminescence signal can be improved, and the accuracy of a detection result is improved.

In some embodiments, the activation time of the light source 30 is prolonged to increase the time for the light to excite the biological sample or the biomolecules carried by the biological sample, and the chemiluminescence signal or the photoluminescence signal can also be improved to improve the accuracy of the detection result.

In some embodiments, the sensor 43 captures images of the biological sample multiple times, and the control unit 50 receives and superposes the multiple images, so as to improve the chemiluminescence signal or the photoluminescence signal, and improve the accuracy of the detection result.

As shown in fig. 1, fig. 2 and fig. 5, preferably, the sample placement layer 20 includes a fixing base 21 and a second rotating disc 22, the fixing base 21 is disposed on the base 10, the second rotating disc 22 is disposed on the fixing base 21, the second rotating disc 22 includes a plurality of detection areas 221 and is electrically connected to the control unit 50 and can rotate relative to the fixing base 21, and a light-transmitting portion 222 is disposed at the bottom of each detection area 221. As shown in fig. 1, fig. 2 and fig. 5, preferably, the photosensitive layer 40 includes a cover 41, a first turntable 42 and a plurality of sensors 43, the cover 41 is located above the sample placement layer 20, the first turntable 42 is disposed on the cover 41 and electrically connected to the control unit 50 and capable of rotating relative to the cover 41, and the plurality of sensors 43 are disposed on the first turntable 42 at intervals and aligned with the plurality of detection regions 221 respectively.

The following description will further describe how the portable biological detection system of the present invention detects a plurality of biological samples in several times with reference to the drawings.

First, as shown in FIG. 2, the cover 41 is opened, and a plurality of carriers 100 containing biological samples are placed on the plurality of detection regions 221. Then, one of the plurality of carriers 100 is selected as a first biological sample, and one of the plurality of sensors 43 is selected as a main sensing element. Then, as shown in fig. 1, the lid 41 is closed. As shown in fig. 3 to 5, the second rotating disc 22 rotates relative to the fixed base 21, so that the other end of the optical fiber 31 or the light source 30 is aligned with one of the plurality of light-transmitting portions 222; the control unit 50 activates the light source 30, and the light of the light source 30 passes through the optical fiber 31 and is emitted from the other end of the optical fiber 31 to one of the plurality of light-transmitting portions 222, or the light of the light source 30 is directly emitted to one of the plurality of light-transmitting portions 222; the light is projected on one of the detection regions 221, so that the biological sample or the to-be-detected biological molecule carried by the biological sample of one of the carrier sheets 100 is excited by the light to generate chemiluminescence or photoluminescence; one of the plurality of sensors 43 receives chemiluminescence or photoluminescence. Finally, the control unit 50 obtains the detection result of the first biological sample according to the chemiluminescence signal or photoluminescence signal.

After the first biological sample is detected, the biological sample of another carrier sheet 100 is selected as the second biological sample, and the main sensing component is still one of the sensors 43. The second rotary table 22 rotates relative to the fixed base 21, so that the other end of the optical fiber 31 or the light source 30 is aligned with the other of the plurality of light-transmitting portions 222; the control unit 50 activates the light source 30, and the light of the light source 30 passes through the optical fiber 31 and is emitted from the other end of the optical fiber 31 to another one of the plurality of light-transmitting portions 222, or the light of the light source 30 is directly emitted to another one of the plurality of light-transmitting portions 222; the light is projected to another one of the detection regions 221, so that the biological sample of another one of the carrier sheets 100 or the biological molecule to be detected carried by the biological sample is excited by the light to generate chemiluminescence or photoluminescence; one of the plurality of sensors 43 receives chemiluminescence or photoluminescence. Finally, the control unit 50 obtains the detection result of the second biological sample according to the chemiluminescence signal or photoluminescence signal.

The detection processes of the rest biological samples are as described above, and so on, and finally the detection results of all the biological samples can be obtained.

In case one of the plurality of sensors 43, which is the main sensing element, is contaminated, the first turntable 42 rotates relative to the cover 41, and the other sensors 43 receive chemiluminescence or photoluminescence as the main sensing elements instead.

In some embodiments, the plurality of sensors 43 sense chemiluminescence or photoluminescence at different wavelengths, respectively. If it is desired to sense chemiluminescence or photoluminescence of a specific wavelength from a biological sample or a biomolecule to be detected carried by a biological sample of one of the carrier sheets 100, the first turntable 42 is rotated relative to the cover 41 so that the sensor 43 specifically sensing chemiluminescence or photoluminescence of a specific wavelength can be aligned with one of the detection zones 221 and receive chemiluminescence or photoluminescence of a specific wavelength from a biological sample or a biomolecule to be detected carried by a biological sample of one of the carrier sheets 100. The control unit 50 obtains a detection result according to the chemiluminescence signal or photoluminescence signal of a specific wavelength.

In some embodiments, first carousel 42 is capable of rotating synchronously with second carousel 22 such that each sensor 43 is capable of receiving chemiluminescence or photoluminescence generated by the biological sample of each carrier sheet 100 or the biomolecules to be detected carried thereby.

In some embodiments, the first turntable 42 is removable from the cover 41, the second turntable 22 is removable from the fixed base 21, and the plurality of sensors 43 are removable from the first turntable 42 to allow replacement of the first turntable 42, the second turntable 22, and the sensors 43.

As shown in fig. 1 and 2, preferably, the cover 41 is pivotally disposed at one side of the fixing base 21, so that the cover 41 is easily opened and closed.

As shown in fig. 2, preferably, the first rotating disc 42 has a plurality of receiving grooves 421, and the plurality of sensors 43 are respectively disposed in the plurality of receiving grooves 421. Therefore, the plurality of receiving grooves 421 can protect the plurality of sensors 43, and prevent the plurality of sensors 43 from being contaminated.

As shown in fig. 2, 3 and 4, the light-transmitting portion 222 is preferably a through hole penetrating through the bottom of each detection area 221. Therefore, the light can penetrate one of the light-transmitting portions 222 to be projected on one of the detection regions 221.

As shown in fig. 1 and fig. 5, the portable biological detecting system of the present invention further includes a display screen 60, the display screen 60 is disposed on one side of the cover 41 opposite to the plurality of sensors 43 and electrically connected to the control unit 50. In some embodiments, the display screen 60 may also be disposed on the outside of the base 10. The control unit 50 can display the detection result and the microscopic image on the display screen 60, so that the user can easily know the detection result and observe the microscopic image from the display screen 60 without needing to shift through other devices, which is quite convenient.

As shown in fig. 5, the control unit 50 may also be connected to a server 70, and the server 70 can receive the detection result and the microscopic image and store the detection result and the microscopic image in a database (not shown) of the server 70. Therefore, the utility model discloses a portable biological detection system can in time send testing result and microscopic image to medical institution's server 70, and medical institution's server 70 can save testing result and microscopic image. When the doctor and the patient make a remote diagnosis, the doctor can directly retrieve the detection result and the microscopic image from the database of the server 70 of the medical institution, and the patient does not need to go to and from the medical institution, thereby saving the cost and time.

Fig. 6 is a perspective view of the second embodiment of the present invention with the cover 41 opened, and fig. 7 is a cross-sectional view taken along line B-B of fig. 6. As shown in fig. 6 and 7, the second embodiment differs from the first embodiment in the structure: the photosensitive layer 40A further includes a plurality of filters 44, and the filters 44 are disposed on a side of the sensors 43 facing the detection regions 221. More specifically, the plurality of filters 44 are respectively disposed in the plurality of receiving slots 421 and close to the openings of the plurality of receiving slots 421. The optical filters 44 can remove light interference signals (especially laser interference signals), so that the chemiluminescence signals or photoluminescence signals are more obvious, and the effect of receiving chemiluminescence or photoluminescence by the sensors 43 is improved, so as to improve the accuracy of the detection result. Otherwise, the remaining technical features of the second embodiment are identical to those of the first embodiment.

In some embodiments, the plurality of filters 44 can be removed from the first carousel 42 so that a new filter 44 can be replaced.

Fig. 8 is a plan view of a photosensitive layer 40B according to a third embodiment of the present invention, and fig. 9 is a schematic structural view of the third embodiment of the present invention. As shown in fig. 8 and 9, the third embodiment differs from the first embodiment in the structure: the photosensitive layer 40B further includes a plurality of objective lenses 45, the plurality of sensors 43 are disposed in pairs with the plurality of objective lenses 45, and the plurality of objective lenses 45 are respectively aligned with the plurality of detection areas 221 and electrically connected to the control unit 50. In other words, one sensor 43 and one objective lens 45 are disposed per accommodation groove 421. When one of the sensors 43 is used as a main sensing element, the objective lens 45 paired with the one of the sensors 43 can be used as a main microscope lens to generate a microscopic image. If there is a need for microscopic observation, the user can observe the state of the biological sample from the microscopic image. Otherwise, the remaining technical features of the third embodiment are identical to those of the first embodiment.

Fig. 10 is a plan view of a photosensitive layer 40C according to a fourth embodiment of the present invention. As shown in fig. 10, the fourth embodiment differs from the third embodiment in the structure: the plurality of sensors 43 are provided separately from the plurality of objective lenses 45. In other words, the plurality of sensors 43 and the plurality of objective lenses 45 are disposed in different accommodating grooves 421. After each biological sample is tested, the first turntable 42 is rotated relative to the cover 41, so that one of the objective lenses 45 can be aligned with one of the detection regions 221 and used as a main microscope lens to generate a microscopic image. If there is a need for microscopic observation, the user can observe the state of the biological sample from the microscopic image. Otherwise, the remaining technical features of the fourth embodiment are identical to those of the third embodiment.

In some embodiments, one of the objective lenses 45 rotates to adjust the Z-axis to the focal plane for focusing, and then the second turntable 22 moves horizontally relative to the fixing base 21, so that one of the objective lenses 45 can align with one of the detection areas 221 and serve as a main microscope lens to generate a microscopic image.

Preferably, the objective lens 45 may be a fixed focus microscope lens or a variable focus microscope lens, and the user can select the form of the objective lens 45 according to the purpose of use.

In some embodiments, the plurality of objective lenses 45 can be removed from the first carousel 42, so that a new objective lens 45 can be replaced.

To sum up, the portable biological detection system of the present invention can perform the whole transformation of the optical path for exciting the light emitted from the light source 30, the biological sample or the biomolecules to be detected carried by the biological sample to generate chemiluminescence or photoluminescence (e.g. fluorescence) and receiving the chemiluminescence or photoluminescence by the sensor 43 through the structural configuration, so as to detect the biological sample or the biomolecules to be detected carried by the biological sample and obtain a good detection result in the state of omitting the spectroscope and reducing the number of the optical filters. Compared with the prior art, the portable biological detection system of the utility model has fewer optical components, and achieves the effects of reducing the volume, reducing the weight, reducing the cost and the like. Therefore, the portable biological detection system of the utility model is suitable for being carried about and used anytime and anywhere, the patient does not need to frequently come and go to the medical institution for detection, and the time and the cost of coming and going of traffic are saved.

The foregoing is illustrative of the preferred embodiment of the present invention and is not intended to limit the invention in any way, and modifications and variations of the invention are possible in light of the above teachings and are within the purview of this application and scope of the invention.

Claims (10)

1. A portable biological detection system, comprising:

a base;

the sample placing layer is arranged on the base and comprises at least one detection area, and the at least one detection area is used for placing a bearing sheet containing a biological sample on the at least one detection area;

the light source is arranged in the base and emits light to the at least one detection area;

a photosensitive layer located above the sample placement layer and comprising at least one sensor aligned with the at least one detection zone; and

and the control unit is electrically connected with the light source and the at least one sensor.

2. The portable biological detection system of claim 1, wherein the sample placement layer comprises a plurality of detection zones, the photosensitive layer comprises a cover, a first turntable and a plurality of sensors, the cover is disposed above the sample placement layer, the first turntable is disposed on the cover and electrically connected to the control unit and capable of rotating relative to the cover, and the plurality of sensors are disposed on the first turntable at intervals and aligned with the plurality of detection zones respectively.

3. The portable biological detection system of claim 1, wherein the sample placement layer comprises:

the light source emits light to one of the light transmission parts, and the light passes through one of the light transmission parts and is projected to one of the detection areas.

4. The portable biological detection system of claim 3, wherein the second turntable is capable of moving horizontally relative to the mounting base.

5. The portable biological detection system of claim 1, wherein the control unit turns off the at least one sensor when the control unit turns on the light source; and wherein the control unit activates the at least one sensor when the control unit turns off the light source.

6. The portable biological detection system of claim 5, wherein the control unit synchronously controls the period of activation and deactivation of the light source and the period of activation and deactivation of the sensor.

7. The portable biological detection system of claim 1, wherein the light source is coupled to one end of an optical fiber, the other end of the optical fiber being aligned with the at least one detection zone; when the light source emits light, the light of the light source passes through the optical fiber and is emitted from the other end of the optical fiber to the at least one detection area.

8. The portable biological detection system of claim 1, wherein the photosensitive layer further comprises:

the at least one optical filter is arranged on one side, facing the at least one detection area, of the at least one sensor.

9. The portable biological detection system of claim 1, wherein the photosensitive layer further comprises:

the at least one objective lens is aligned with the at least one detection area and is electrically connected with the control unit.

10. The portable biological detection system of claim 1, further comprising:

and the display screen is arranged on the base or the photosensitive layer and is electrically connected with the control unit.

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