CN217265803U - Novel cell culture solution provide in real time device - Google Patents
Novel cell culture solution provide in real time device Download PDFInfo
- Publication number
- CN217265803U CN217265803U CN202220793317.5U CN202220793317U CN217265803U CN 217265803 U CN217265803 U CN 217265803U CN 202220793317 U CN202220793317 U CN 202220793317U CN 217265803 U CN217265803 U CN 217265803U
- Authority
- CN
- China
- Prior art keywords
- sleeve
- culture solution
- movable rod
- cell culture
- fixedly connected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a novel real-time cell culture solution supply device, which comprises a culture dish, the outer surface of the culture dish is fixedly connected with a fixed sleeve through a connecting plate, the inner surface of the fixed sleeve is provided with a movable rod in a sliding way, the movable rod is L-shaped, an adjusting mechanism is arranged between the fixed sleeve and the movable rod, one end of the movable rod far away from the fixed sleeve is fixedly connected with a connecting sleeve, the connecting sleeve is positioned above the culture dish, the connecting sleeve is clamped with the culture solution collecting bottle, the utility model is provided with the culture dish, the outer wall of the culture dish is fixedly connected with a fixed sleeve through a connecting plate, the inner surface of the fixed sleeve is provided with a movable rod in a sliding way, the movable rod is connected with the connecting sleeve, the culture solution collecting bottle is clamped at the connecting sleeve, and the culture solution in the culture solution collecting bottle can flow into the culture dish to supplement the culture solution in the culture dish in real time.
Description
Technical Field
The utility model relates to a culture solution provides technical field, and concrete field is a novel cell culture solution provide device in real time.
Background
With the development of biotechnology, the field of cell culture is rapidly developed. Cell culture is an important branch of regenerative medicine and life science, and when culturing cells, a cell culture device is concerned with important factors for culturing cells. In the prior art, there are several common devices for culturing cells:
chinese patent application 201720741150.7 discloses a multifunctional cell culture device, which comprises a first cell culture platform, a second cell culture platform, a left clapboard and a right clapboard, wherein the left clapboard and the right clapboard are respectively arranged in a box body in parallel in the longitudinal direction, the left clapboard, the right clapboard and the side wall of the box body respectively form a left cell culture zone, a middle cell culture zone and a right cell culture zone, a first cell culture tank is arranged on the inner bottom surface of the left cell culture zone, the first cell culture tank comprises two circular grooves and a cell migration tank, and the two circular grooves are communicated through the cell migration tank; and a second cell culture tank is arranged in the middle cell culture area, a third cell culture tank is arranged in the right cell culture area, and the second cell culture tank and the third cell culture tank are both identical to the first cell culture tank in structure.
Chinese patent application 201520354728.4 discloses a dual-chamber permselective cell culture device, as shown in fig. 1, comprising: at least 2 cell culture chambers: a first cell culture chamber and a second cell culture chamber; wherein the first cell culture chamber is used for culturing feeder cells capable of secreting special substances, and the second cell culture chamber is used for receiving special substances to culture target cells; the first cell culture chamber is connected with the second cell culture chamber through a semi-permeable membrane; and the first cell culture chamber and the second cell culture chamber are both provided with a liquid inlet channel and an inoculation channel.
When the culture solution of the above culture devices is supplemented, manual control is required, and the nutrient solution cannot be automatically supplemented, so that a novel cell culture solution real-time providing device is provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a novel cell culture liquid provide device in real time to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a novel cell culture liquid provide in real time device, includes the culture dish, the surface of culture dish passes through connecting plate fixedly connected with fixed sleeve, fixed sleeve's internal surface slides and is equipped with the movable rod, the movable rod is the L type, fixed sleeve with be equipped with adjustment mechanism between the movable rod, the movable rod is kept away from fixed sleeve's one end fixedly connected with connecting sleeve, just connecting sleeve is located the culture dish top, the connecting sleeve joint has the culture solution receiving flask.
Preferably, the cell phone screen for the culture solution comprises a bottle body and a bottle opening, wherein the bottle opening is arranged at the lower end of the bottle body in a penetrating mode, two limiting rings are fixedly connected to two ends of the inner surface of the bottle opening respectively, a movable pipe is arranged between the two limiting rings in a sliding mode, the upper end of the movable pipe is provided with an opening, a through hole is formed in the side wall of the movable pipe in a penetrating mode, an annular groove is coaxially formed in the inner surface of the bottle opening, a spring is arranged between the movable pipe and the limiting rings, a push rod is arranged inside the connecting sleeve and is coaxially arranged with the connecting sleeve, and the push rod is fixedly connected with the connecting sleeve through a connecting rod.
Preferably, the number of the connecting rods is multiple, and the connecting rods are respectively arranged in an inclined manner.
Preferably, the outer surface of bottleneck is equipped with the spread groove, the quantity of spread groove is two, two the spread groove symmetry sets up, the upper end lateral wall of spread groove is equipped with the fixed slot respectively, connecting sleeve's internal surface corresponds spread groove department fixedly connected with connecting block respectively.
Preferably, the adjusting mechanism comprises a rotating sleeve, the rotating sleeve is rotatably connected to one end of the fixed sleeve, an external thread is arranged on the outer surface of the movable rod, and an internal thread meshed with the movable rod is arranged on the inner surface of the rotating sleeve.
Preferably, the outer surface of the movable rod is provided with a sliding groove, and the inner surface of the fixed sleeve corresponds to the sliding groove and is fixedly connected with a sliding block.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model provides a novel cell culture liquid provide in real time device, through setting up the culture dish, the culture dish outer wall passes through connecting plate fixedly connected with adapter sleeve, and the sliding of adapter sleeve internal surface sets up the movable rod, and the movable rod is connected with connecting sleeve, at connecting sleeve department joint culture solution receiving flask, and the culture liquid in the culture solution receiving flask then can flow in the culture dish, carries out real-time replenishment to the culture liquid in the culture dish.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic view of the front view cross-sectional structure of the present invention;
FIG. 3 is a schematic view of the cross-sectional structure of the culture solution collecting bottle according to the present invention;
FIG. 4 is a schematic view of the structure of the bottle mouth of the present invention;
fig. 5 is a schematic top view of the cross-sectional structure of the fixing sleeve according to the present invention.
In the figure: 1-culture dish, 2-connecting plate, 3-fixed sleeve, 4-movable rod, 5-connecting sleeve, 6-bottle body, 7-bottle mouth, 8-spacing ring, 9-movable tube, 10-through hole, 11-annular groove, 12-spring, 13-top rod, 14-connecting rod, 15-connecting groove, 16-fixed groove, 17-connecting block, 18-rotary sleeve, 19-sliding groove and 20-sliding block.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: a novel cell culture solution real-time providing device comprises a culture dish 1, wherein the culture dish 1 is a commercially available ordinary culture dish, a fixed sleeve 3 is fixedly connected to the outer surface of the culture dish 1 through a connecting plate 2, the axis of the fixed sleeve 3 is vertically arranged, a movable rod 4 is arranged on the inner surface of the fixed sleeve 3 in a sliding manner, the movable rod 4 can slide up and down in the fixed sleeve 3, the movable rod 4 is L-shaped, an adjusting mechanism is arranged between the fixed sleeve 3 and the movable rod 4 and used for adjusting the relative position between the movable rod 4 and the fixed sleeve 3, one end, far away from the fixed sleeve 3, of the movable rod 4 is fixedly connected with a connecting sleeve 5, the connecting sleeve 5 is positioned above the culture dish 1, a culture solution collecting bottle is clamped in the connecting sleeve, as shown in figure 1, the culture solution collecting bottle is inverted, the bottle mouth is the height of the liquid level of the culture solution in the culture dish 1.
Specifically, the culture solution collecting bottle comprises a bottle body 6 and a bottle mouth 7, the bottle mouth 7 is arranged at the lower end of the bottle body 6 in a penetrating manner, two ends of the inner surface of the bottle mouth 7 are fixedly connected with limit rings 8 respectively, a movable tube 9 is arranged on the inner surface of the bottle mouth 7 corresponding to the position between the two limit rings 8 in a sliding manner, the upper end of the movable tube 9 is in an open shape, the side wall of the movable tube 9 is provided with a through hole 10 in a penetrating manner, the inner surface of the bottle mouth 7 is coaxially provided with an annular groove 11, a spring 12 is arranged between the movable tube 9 and the limit rings 8, the movable tube 9 is positioned at the lower end of the bottle mouth 7 through the spring 12, so that the movable tube 9 can block the bottle mouth 7 to prevent the culture solution from flowing out when the culture solution collecting bottle is filled with the culture solution under normal conditions, a mandril 13 is arranged inside the connecting sleeve 5, and the mandril 13 is arranged coaxially with the connecting sleeve 5, the ejector rod 13 is fixedly connected with the connecting sleeve 5 through a connecting rod 14, and after the bottle mouth 7 and the connecting sleeve 5 are connected, the ejector rod 13 jacks up the movable tube 9, so that the culture solution can enter the annular groove 11 through the through hole 10 and finally flows out of the bottle mouth 7, and the culture solution is communicated with the culture dish 1.
Specifically, the number of the connecting rods 14 is plural, and the connecting rods 14 are respectively arranged obliquely, so that the connecting rods 14 receive less stress when being installed, and the durability of the device is increased.
Particularly, the surface of bottleneck 7 is equipped with connecting groove 15, the quantity of connecting groove 15 is two, two connecting groove 15 symmetry sets up, the upper end lateral wall of connecting groove 15 is equipped with fixed slot 16 respectively, the internal surface of connecting sleeve 5 corresponds fixedly connected with connecting block 17 respectively in connecting groove 15 punishment, connecting groove 15 forms L type groove with fixed slot 16, and during the installation, connecting block 17 aims at connecting groove 15, and when connecting block 17 removed to fixed slot 16 department, rotation bottleneck 7 for connecting block 17 slides in fixed slot 16, is connected bottleneck 7 and connecting sleeve 5.
Particularly, adjustment mechanism includes rotatory sleeve 18, rotatory sleeve 18 rotates to be connected the one end of fixed sleeve 3, the surface of movable rod 4 is equipped with the external screw thread, the internal surface of rotatory sleeve 18 be equipped with the internal thread of movable rod 4 meshing to rotate rotatory sleeve 18, drive movable rod 4 and reciprocate under the screw-thread fit effect, thereby drive connecting sleeve 5 and slide from top to bottom, and then change bottleneck 7 height, make and to adjust culture solution liquid level height in the culture dish 1.
Specifically, the outer surface of the movable rod 4 is provided with a sliding groove 19, the inner surface of the fixed sleeve 3 is fixedly connected with a sliding block 20 corresponding to the sliding groove 19, and when the movable rod 4 slides in the fixed sleeve 3, the sliding block 20 slides in the sliding groove 19, so that when the rotating sleeve 18 rotates, the movable rod 4 cannot slide along with the sliding block.
The working principle is as follows: when the utility model is used, the culture dish 1 is used for cell culture, the culture solution is filled in the culture solution collecting bottle, the bottle mouth 7 of the culture solution collecting bottle faces downwards and is inserted by the connecting sleeve 5, so that the connecting block 17 enters the connecting groove 15, when sliding to the fixed groove 16, the bottle mouth 7 is rotated, the connecting block 17 enters the fixed groove 16 to complete the fixation, the mandril 13 props against the movable tube 9 at the moment, the culture solution in the bottle body 6 flows into the annular groove 11 from the through hole 10 and finally flows out from the bottle mouth 7, so that the culture solution in the bottle body 6 is communicated with the culture solution in the culture dish 1, so that after the culture solution in the culture dish 1 is lost, the culture solution in the bottle body 6 can enter the culture dish 1 for supplement, and by rotating the rotary sleeve 18, can drive the movable rod 4 to move up and down under the screw-thread fit effect to change the height of the bottle mouth 7 and realize the adjustment of the liquid level of the culture solution in the culture dish 1.
In the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The utility model discloses the standard part that uses all can purchase from the market, and dysmorphism piece all can be customized according to the description with the record of drawing of description, and the concrete connection mode of each part all adopts conventional means such as ripe bolt, rivet, welding among the prior art, and machinery, part and equipment all adopt prior art, and conventional model, including circuit connection adopts conventional connection mode among the prior art, does not detailed here again.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a novel cell culture liquid provide device in real time, includes culture dish (1), its characterized in that: the surface of culture dish (1) passes through connecting plate (2) fixedly connected with fixed sleeve (3), the internal surface slip of fixed sleeve (3) is equipped with movable rod (4), movable rod (4) are the L type, fixed sleeve (3) with be equipped with adjustment mechanism between movable rod (4), movable rod (4) are kept away from the one end fixedly connected with connecting sleeve (5) of fixed sleeve (3), just connecting sleeve (5) are located culture dish (1) top, the connecting sleeve joint has the culture solution receiving flask.
2. The novel device for providing a cell culture solution in real time according to claim 1, wherein: the culture solution collecting bottle comprises a bottle body (6) and a bottle mouth (7), the bottle mouth (7) is arranged at the lower end of the bottle body (6) in a penetrating way, two ends of the inner surface of the bottle mouth (7) are respectively fixedly connected with a limiting ring (8), a movable pipe (9) is arranged on the inner surface of the bottle mouth (7) corresponding to the position between the two limiting rings (8) in a sliding way, the upper end of the movable tube (9) is open, the side wall of the movable tube (9) is provided with a through hole (10) in a penetrating way, an annular groove (11) is coaxially arranged on the inner surface of the bottle mouth (7), a spring (12) is arranged between the movable tube (9) and the limiting ring (8), a mandril (13) is arranged in the connecting sleeve (5), the mandril (13) and the connecting sleeve (5) are coaxially arranged, the ejector rod (13) is fixedly connected with the connecting sleeve (5) through a connecting rod (14).
3. The novel device for providing a cell culture solution in real time as claimed in claim 2, wherein: the number of the connecting rods (14) is multiple, and the connecting rods (14) are obliquely arranged respectively.
4. The novel device for providing a cell culture solution in real time according to claim 3, wherein: the surface of bottleneck (7) is equipped with connecting groove (15), the quantity of connecting groove (15) is two, two connecting groove (15) symmetry sets up, the upper end lateral wall of connecting groove (15) is equipped with fixed slot (16) respectively, the internal surface of connecting sleeve (5) corresponds connecting groove (15) punishment difference fixedly connected with connecting block (17).
5. The novel device for providing a cell culture solution in real time according to claim 1, wherein: adjustment mechanism includes rotatory sleeve (18), rotatory sleeve (18) rotate to be connected the one end of fixed sleeve (3), the surface of movable rod (4) is equipped with the external screw thread, the internal surface of rotatory sleeve (18) be equipped with the internal thread of movable rod (4) meshing.
6. The novel device for providing a cell culture solution in real time as claimed in claim 5, wherein: the outer surface of the movable rod (4) is provided with a sliding groove (19), and the inner surface of the fixed sleeve (3) corresponds to the sliding groove (19) and is fixedly connected with a sliding block (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220793317.5U CN217265803U (en) | 2022-04-07 | 2022-04-07 | Novel cell culture solution provide in real time device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220793317.5U CN217265803U (en) | 2022-04-07 | 2022-04-07 | Novel cell culture solution provide in real time device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217265803U true CN217265803U (en) | 2022-08-23 |
Family
ID=82873488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220793317.5U Active CN217265803U (en) | 2022-04-07 | 2022-04-07 | Novel cell culture solution provide in real time device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217265803U (en) |
-
2022
- 2022-04-07 CN CN202220793317.5U patent/CN217265803U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN217265803U (en) | Novel cell culture solution provide in real time device | |
| CN111043439B (en) | Exhaust device of water supply pipeline | |
| CN209200063U (en) | A kind of lithium battery assembly line priming device | |
| CN213623250U (en) | Automatic filling structure of shampoo | |
| CN211870333U (en) | Waste liquid collecting tank for biological research | |
| CN218546265U (en) | Slurry sampling device for lithium iron phosphate | |
| CN209495880U (en) | A kind of environmental protection waste water treatment detection sampling apparatus | |
| CN116726841A (en) | Preparation method and preparation device of lithium battery electrolyte additive | |
| CN217846894U (en) | Carbon powder bottle easy for adding carbon powder into selenium drum | |
| CN202853940U (en) | Port blocking and bar holding type fixed-point water sampler | |
| CN213643312U (en) | Simple liquid flow divider | |
| CN210645222U (en) | Purification device in production process of medical intermediate | |
| CN211085818U (en) | Water quality sampling equipment for hydrology and water resource engineering | |
| CN209270892U (en) | A kind of accurate separator of chemical waste liquid | |
| CN215694090U (en) | Pneumatic supernatant liquid taking device | |
| CN111735658A (en) | River water sampling device for treatment | |
| CN220752117U (en) | Automatic clear up formula water quality monitoring device | |
| CN219239644U (en) | Plant source extract insecticidal synergist fermentation ware | |
| CN216645981U (en) | Air detection sampling device | |
| CN217284376U (en) | Nutrient solution self-circulation water culture device | |
| CN204798908U (en) | Modified stiff ware device | |
| CN220070916U (en) | Filter equipment is used in lubricating oil processing | |
| CN117516993B (en) | Monitoring device and monitoring method for mine geological environment | |
| CN209905580U (en) | Metering tank | |
| CN214031683U (en) | Automatic quantitative filling device of fruit juice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |