CN217156434U

CN217156434U - Liquid chromatography equipment

Info

Publication number: CN217156434U
Application number: CN202123150552.5U
Authority: CN
Inventors: 吴文琴
Original assignee: Acc Quality Detection Technology Shanghai Co ltd
Current assignee: Acc Quality Detection Technology Shanghai Co ltd
Priority date: 2021-12-15
Filing date: 2021-12-15
Publication date: 2022-08-09
Anticipated expiration: 2031-12-15

Abstract

The utility model discloses a liquid chromatography equipment, it includes: solvent case, junction box, advance kind case, notes incubator and detection case, solvent case, junction box, advance kind case, notes incubator and detection case follow and set gradually down, the junction box is including locating the first box of solvent case lower part and being located inside discharge valve and the first transmission valve of first box, the solvent bottle is connected with discharge valve through first pipeline, discharge valve passes through the second pipeline and is connected with first transmission valve. The utility model discloses it can enough solve the problem that chromatographic column can not be connected to the detector in the past, can also replace the mode that adopts expensive metal column as chromatographic column in the past, and this connecting pipe is with low costs, can the wide use.

Description

Liquid chromatographic analysis equipment

Technical Field

The utility model relates to a chromatographic analysis technical field, especially a liquid chromatographic analysis equipment.

Background

As the most important analysis and separation means of organic compounds, high performance liquid chromatography is widely applied in the fields of pharmacy, medical treatment, food, chemical industry and the like, and the liquid chromatograph mainly comprises: the liquid chromatographic analyzer is an instrument which utilizes the difference of the distribution ratio of a mixture between liquid and solid or immiscible liquid to sequentially separate and identify the mixture, and for the chromatographic column, the compound is subjected to the precipitation action of the column to achieve the detection purpose, mainly to detect whether a certain component exists and quantify the component.

Based on the above, we propose a liquid chromatography apparatus to solve the above problems.

SUMMERY OF THE UTILITY MODEL

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and in the abstract of the specification and the title of the application to avoid obscuring the purpose of this section, the abstract of the specification and the title of the application, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above and/or other problems occurring in the conventional chromatographic analyzer.

Therefore, one of the objects of the present invention is to provide a liquid chromatography apparatus, which can solve the problem that the conventional chromatographic column cannot be connected to the detector, and can replace the conventional method of using an expensive metal column as the chromatographic column, and the connecting pipe is low in cost and can be widely used.

In order to achieve the above purpose, the utility model provides a following technical scheme: a liquid chromatography apparatus, comprising: solvent case, junction box, advance kind case, notes incubator and detection case, solvent case, junction box, advance kind case, notes incubator and detection case follow and set gradually down, the junction box is including locating the first box of solvent case lower part and being located inside discharge valve and the first transmission valve of first box, the solvent bottle is connected with discharge valve through first pipeline, discharge valve passes through the second pipeline and is connected with first transmission valve.

As a preferred embodiment of the present invention, wherein: the sample injection box comprises a second box body arranged on the lower portion of the first box body, a movable assembly arranged inside the second box body and a second transmission valve arranged inside the second box body, wherein one end of the movable assembly is provided with a needle tube, one end, far away from the needle point, of the needle tube is provided with an absorption valve, the second transmission valve is provided with a first connecting port, a second connecting port and a third connecting port, the absorption valve is connected with the first connecting port through a third pipeline, the first transmission valve is connected with the second connecting port through a fourth pipeline, and the third connecting port is connected with the temperature injection box.

As a preferred embodiment of the present invention, wherein: the moving assembly comprises a rotating disc fixedly connected with the second box body, the rotating disc is provided with a protrusion, the protrusion is connected with a ring block in a sliding mode, connecting rods are arranged at two ends of the ring block, a sliding groove is vertically formed in the second box body, the connecting rods are connected with the sliding groove through sliding blocks, the ring block is fixedly connected with a moving rod, a clamp is arranged at one end of the moving rod, and the needle tube is arranged inside the clamp.

As a preferred embodiment of the present invention, wherein: annotate the incubator including locating the third box of second box lower part, being located inside the third box purifies the post and is located the inside connecting pipe of third box, purify post one end and pass through fifth pipeline and third connector connection, purify the post other end and connecting pipe threaded connection, the connecting pipe passes through the sixth pipeline and is connected with the detection case.

As a preferred embodiment of the present invention, wherein: the temperature injection box is characterized in that a fixing plate is arranged in the temperature injection box, the fixing plate is provided with a first clamping seat and a second clamping seat, the purifying column is clamped with the first clamping seat, and the connecting pipe is clamped with the second clamping seat.

As a preferred embodiment of the present invention, wherein: the inside first sample board and the second sample board that still are equipped with of second box, first sample board is equipped with first sample groove, the second sample board is equipped with second sample groove, second sample groove is located the needle tubing below.

As a preferred embodiment of the present invention, wherein: the suction valve comprises a valve body and a piston located inside the valve body, one side of the valve body is connected with the first connecting port through a third pipeline, and one end of the valve body is connected with the needle tube through a seventh pipeline.

As a preferred embodiment of the present invention, wherein: the needle tubing outside is equipped with anti-skidding circle, anchor clamps and anti-skidding circle contact are connected.

The utility model has the advantages that: the utility model discloses a purifying column and connecting pipe constitute the chromatographic column jointly, through pipe connection to detector, the inside screw thread that is provided with of connecting pipe, the mode that adopts the connecting pipe to connect purifying column can replace the problem that the chromatographic column can not be connected to the detector in the past to and can replace the mode that adopts expensive metal column as the chromatographic column in the past, this connecting pipe is with low costs, can the wide use.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive labor. Wherein:

fig. 1 is an overall structure diagram of the present invention.

Fig. 2 is a structural diagram of the connection box and the sampling box of the present invention.

Fig. 3 is a structural diagram of the moving assembly of the present invention.

Fig. 4 is a structural diagram of the heat injection box of the present invention.

Fig. 5 is a connecting structure diagram of the needle tube, the suction valve and the second transfer valve according to the present invention.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanying the present invention are described in detail below with reference to the accompanying drawings.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be implemented in other ways different from the specific details set forth herein, and one skilled in the art may similarly generalize the present invention without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, the references herein to "one embodiment" or "an embodiment" refer to a particular feature, structure, or characteristic that may be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

Example 1

Referring to fig. 1 and 2, in order to provide a first embodiment of the present invention, the embodiment provides a liquid chromatography apparatus, which includes a solvent tank 100, a connection tank 200, a sample introduction tank 300, an incubator 400, and a detection tank 500. The connection box 200 is used for discharging bubbles in the flowing solvent, the sample injection box 300 is used for sampling and transmitting the samples to the temperature injection box 400, the temperature injection box 400 is used for adjusting and maintaining a certain temperature, the detector 500 is used for detecting components and content thereof in a certain sample, and the chromatographic analyzer can be used for efficiently detecting and obtaining corresponding results, such as determination of immunoglobulin IgG in health-care food.

The solvent tank 100 is internally provided with a solvent bottle 101, the connecting tank 200 comprises a first tank body 201 arranged at the lower part of the solvent tank 100, an exhaust valve 202 and a first transmission valve 203, the exhaust valve 202 and the first transmission valve 203 are positioned in the first tank body 201, the solvent bottle 101 is connected with the exhaust valve 202 through a first pipeline 101a, the exhaust valve 202 is connected with the first transmission valve 203 through a second pipeline 202a, the sample injection tank 300 is arranged at the lower part of the first tank body 201, the temperature injection tank 400 is arranged at the lower part of the sample injection tank 300, and the detection tank 500 is arranged at the lower part of the temperature injection tank 400.

The present embodiment provides two solvent bottles 101, the solvent inside the solvent bottle 101 passes through the first pipe 101a to the exhaust valve 202, the exhaust valve 202 is used for exhausting the air bubbles inside the solvent, the bubble-free solvent is transferred to the first transfer valve 203, and the first transfer valve 203 is used for transferring the solvent to the second tank 301.

Example 2

Referring to fig. 1 to 5, a second embodiment of the present invention is based on the above embodiment.

The sample introduction box 300 comprises a second box body 301 arranged at the lower part of the first box body 201, a moving assembly 600 arranged in the second box body 301 and a second transmission valve 302 arranged in the second box body 301, wherein one end of the moving assembly 600 is provided with a needle tube 303, one end of the needle tube 303 far away from the needle point is provided with a suction valve 304, the second transmission valve 302 is provided with a first connecting port 302a, a second connecting port 302b and a third connecting port 302c, the suction valve 304 is connected with the first connecting port 302a through a third pipeline 304a, the first transmission valve 203 is connected with the second connecting port 302b through a fourth pipeline 203a, and the third connecting port 302c is connected with the temperature injection box 400.

The moving assembly 600 is used to move the needle tube 303 up and down, the needle tube 303 is upward for placing a sample bottle on the first sample plate 305, the needle tube 303 is downward for inserting a needle into the sample bottle, the second transfer valve 302 is used to transfer a solvent and the sample to the purifying column 402, the suction valve 304 is used to suck the sample inside the sample to the second transfer valve 302, and the solvent is transferred from the first transfer valve 203 to the second transfer valve 302 through the fourth pipe 203 a.

The moving assembly 600 comprises a rotating disc 601 fixedly connected with the second box body 301, the rotating disc 601 is provided with a protrusion 602, the protrusion 602 is slidably connected with a ring block 603, two ends of the ring block 603 are provided with connecting rods 604, the second box body 301 is vertically provided with a sliding groove 305, the connecting rods 604 are connected with the sliding groove 305 through sliding blocks, the ring block 603 is fixedly connected with a moving rod 605, one end of the moving rod 605 is provided with a clamp 606, and the needle tube 303 is arranged inside the clamp 606.

The turntable 601 is driven by a motor, the protrusion 602 rotates together with the turntable 601, the ring block 603 slides up and down in the sliding groove 305 through the connecting rod 604, the ring block 603 is limited by the sliding groove 305 and the connecting rod 604, the ring block 603 can move up and down in a reciprocating mode due to the rotation of the turntable 601, and the clamp 606 is used for fixedly clamping the needle tube 303.

The incubator 400 includes a third casing 401 disposed at a lower portion of the second casing 301, a purification column 402 disposed inside the third casing 401, and a connection pipe 403 disposed inside the third casing 401, one end of the purification column 402 is connected to the third connection port 302c through a fifth pipe 402a, the other end of the purification column 402 is screwed to the connection pipe 403, and the connection pipe 403 is connected to the detection chamber 500 through a sixth pipe 403 a.

The purifying column 402 and the connecting pipe 403 jointly form a chromatographic column, the connecting pipe 403 is internally provided with threads, the purifying column 402 is externally provided with threads, the mode of connecting the purifying column 402 by the connecting pipe 403 can replace the problem that the conventional chromatographic column cannot be connected to a detector and the mode of using an expensive metal column as the chromatographic column can be replaced, the connecting pipe 403 is low in cost and can be widely used, the connecting pipe 403 is two-way with zero dead volume, and the product number is Agilent Part No 5022 and 2145 and is made of stainless steel.

The third housing 401 has a fixing plate 404, the fixing plate 404 has a first clamping seat 405 and a second clamping seat 406, the purifying column 402 is clamped with the first clamping seat 405, and the connecting tube 403 is clamped with the second clamping seat 406. The fixing plate 404 is used for fixing a first clamping seat 405 and a second clamping seat 406, the first clamping seat 405 is used for detachably fixing the purifying column 402, and the second clamping seat 406 is used for detachably fixing the connecting pipe 403.

The second casing 301 is further provided with a first sample plate 305 and a second sample plate 306, the first sample plate 305 is provided with a first sample slot 305a, the second sample plate 306 is provided with a second sample slot 306a, and the second sample slot 306a is located below the needle tube 303. The first sample well 305a is provided with a plurality of sample bottles, and the second sample well 306a is provided with one sample bottle moved from the first sample well 305a by a robot.

Example 3

Referring to fig. 1 to 5, a third embodiment of the present invention is based on the previous embodiment.

The suction valve 304 includes a valve body 304b and a piston 304c located inside the valve body 304b, one side of the valve body 304b is connected to the first connection port 302a through a third pipe 304a, and one end of the valve body 304b is connected to the needle pipe 303 through a seventh pipe 304 b-1. The piston 304c moves for sucking the sample and transfers to the second transfer valve 302 through the third pipe 304 a.

The outer side of the needle tube 303 is provided with an anti-slip ring 307, and the clamp 606 is in contact connection with the anti-slip ring 307. The anti-slip ring 307 is used to increase the friction between the clamp 606 and the needle tube 303, which is beneficial to increase the clamping force of the clamp 606 on the needle tube 303 without damaging the needle tube 303.

The working principle is as follows:

the solvent is exhausted through the first pipe 101a to the exhaust valve 202, the solvent is transferred into the second transfer valve 302 through the second pipe 202a and the first transfer valve 203, the turntable 601 is rotated, so that the moving rod 605 and the clamp 606 move the needle tube 303 upwards, the sample bottle is moved to the second sample groove 306a through the manipulator inside the second box 301, the moving rod 605 moves downwards so that the needle of the needle tube 303 is inserted into the sample bottle, the sample inside the sample bottle is sucked to the valve body 304b through the seventh pipe 304b-1 by the suction valve 304, the moving rod 605 moves upwards, the sample bottle is removed by the manipulator, the sample is transferred to the second transfer valve 302 through the third pipe 304a, the sample and the solvent are merged inside the second transfer valve 302, and at this time, the sample and the solvent are transferred into the chromatographic column through the fifth pipe 402a and then transferred to the detector 500 through the chromatographic column, and the sample is detected.

It is important to note that the construction and arrangement of the present application as shown in the various exemplary embodiments is illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications are possible (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters (e.g., temperatures, pressures, etc.), mounting arrangements, use of materials, colors, orientations, etc.) without materially departing from the novel teachings and advantages of the subject matter recited in this application. For example, elements shown as integrally formed may be constructed of multiple parts or elements, the position of elements may be reversed or otherwise varied, and the nature or number of discrete elements or positions may be altered or varied. Accordingly, all such modifications are intended to be included within the scope of this invention. The order or sequence of any process or method steps may be varied or re-sequenced according to alternative embodiments. In the claims, any means-plus-function clause is intended to cover the structures described herein as performing the recited function and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments without departing from the scope of the present inventions. Therefore, the present invention is not limited to a particular embodiment, but extends to various modifications that nevertheless fall within the scope of the appended claims.

Moreover, in an effort to provide a concise description of the exemplary embodiments, all features of an actual implementation may not be described (i.e., those unrelated to the presently contemplated best mode of carrying out the invention, or those unrelated to enabling the invention).

It should be appreciated that in the development of any such actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort might be complex and time consuming, but would nevertheless be a routine undertaking of design, fabrication, and manufacture for those of ordinary skill having the benefit of this disclosure, without undue experimentation.

It should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the scope of the claims of the present invention.

Claims

1. A liquid chromatography apparatus, characterized by: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

a solvent tank (100) in which a solvent bottle (101) is provided;

the connecting box (200) comprises a first box body (201) arranged at the lower part of the solvent box (100), and an exhaust valve (202) and a first transmission valve (203) which are positioned in the first box body (201), wherein the solvent bottle (101) is connected with the exhaust valve (202) through a first pipeline (101a), and the exhaust valve (202) is connected with the first transmission valve (203) through a second pipeline (202 a);

a sample introduction box (300) arranged at the lower part of the first box body (201);

the temperature injection box (400) is arranged at the lower part of the sample injection box (300);

and the detection box (500) is arranged at the lower part of the heat injection box (400).

2. The liquid chromatography apparatus of claim 1, wherein: it includes second box (301) of locating first box (201) lower part, is located to advance appearance case (300) second box (301) inside removal subassembly (600) and be located the inside second transmission valve (302) of second box (301), it is equipped with needle tubing (303) to remove subassembly (600) one end, the one end that the needle point was kept away from in needle tubing (303) is equipped with suction valve (304), second transmission valve (302) are equipped with first connector (302a), second connector (302b) and third connector (302c), suction valve (304) are connected with first connector (302a) through third pipeline (304a), first transmission valve (203) are connected with second connector (302b) through fourth pipeline (203a), third connector (302c) are connected with notes temperature case (400).

3. The liquid chromatography apparatus of claim 2, wherein: the moving assembly (600) comprises a rotating disc (601) fixedly connected with the second box body (301), the rotating disc (601) is provided with a protrusion (602), the protrusion (602) is slidably connected with a ring block (603), two ends of the ring block (603) are provided with connecting rods (604), the second box body (301) is vertically provided with a sliding groove (301a), the connecting rods (604) are connected with the sliding groove (301a) through sliding blocks, the ring block (603) is fixedly connected with a moving rod (605), one end of the moving rod (605) is provided with a clamp (606), and the needle tube (303) is arranged inside the clamp (606).

4. The liquid chromatography apparatus of claim 3, wherein: annotate warm case (400) including locating third box (401) of second box (301) lower part, be located purification post (402) and connecting pipe (403) that are located third box (401) inside, purification post (402) one end is connected with third connector (302c) through fifth pipeline (402a), purification post (402) other end and connecting pipe (403) threaded connection, connecting pipe (403) are connected with detection case (500) through sixth pipeline (403 a).

5. The liquid chromatography apparatus of claim 4, wherein: a fixing plate (404) is arranged in the third box body (401), the fixing plate (404) is provided with a first clamping seat (405) and a second clamping seat (406), the purifying column (402) is clamped with the first clamping seat (405), and the connecting pipe (403) is clamped with the second clamping seat (406).

6. The liquid chromatography apparatus of claim 5, wherein: the second box body (301) is also internally provided with a first sample plate (305) and a second sample plate (306), the first sample plate (305) is provided with a first sample groove (305a), the second sample plate (306) is provided with a second sample groove (306a), and the second sample groove (306a) is positioned below the needle tube (303).

7. A liquid chromatography apparatus according to any one of claims 3 to 6, wherein: the suction valve (304) comprises a valve body (304b) and a piston (304c) positioned in the valve body (304b), one side of the valve body (304b) is connected with the first connecting port (302a) through a third pipeline (304a), and one end of the valve body (304b) is connected with the needle tube (303) through a seventh pipeline (304 b-1).

8. The liquid chromatography apparatus of claim 7, wherein: the outer side of the needle tube (303) is provided with an anti-skid ring (307), and the clamp (606) is in contact connection with the anti-skid ring (307).