CN217100976U - Kit for enzyme-linked immunosorbent assay - Google Patents

Abstract

The utility model relates to a kit for enzyme-linked immunosorbent assay, the articulated lid that is connected with in box body upper portion, the bottom surface of lid is provided with the embedded groove that is used for embedding description card, the inside inner baffle that is provided with of box body, the inner baffle divides the box body inside from a left side to the right side into the apparatus district, reagent bottle district and auxiliary area, the box body bottom is provided with the opening and is located the left ELIAS plate of box body and holds the chamber, the articulated chamber lid that is connected with in opening part bottom that the ELIAS plate held the chamber, the ELIAS plate holds the long limit lateral wall in chamber on parallel arrangement have with the isometric guide rail of lateral wall, sliding connection has the slider on the guide rail, the slider is connected with the one end of spring, the other end of spring is connected on the minor face lateral wall that the ELIAS plate held the chamber, the ELIAS plate holds the chamber and is used for holding the ELIAS plate. The kit uniformly accommodates various articles required by the experiment, can be conveniently taken, simultaneously ensures the safety and the universality of carrying and using, and can carry out complete enzyme-linked immunoassay experiments.

Description

Kit for enzyme-linked immunosorbent assay

Technical Field

The utility model belongs to the technical field of the kit, concretely relates to a kit for ELISA.

Background

The enzyme linked immunosorbent assay kit is a detection tool which is most widely applied in the enzyme linked immunosorbent assay technology, and the basic method of the enzyme linked immunosorbent assay is to adsorb known antigen or antibody on the surface of a solid phase carrier, so that the antigen-antibody reaction marked by enzyme is carried out on the surface of the solid phase carrier, and then free components in a liquid phase are washed away by a washing method; the common ELISA (enzyme-linked immunosorbent assay) methods include a double antibody sandwich method for detecting a macromolecular antigen and an indirect method for detecting a specific antibody.

The enzyme-linked immunoassay kit used at the present stage usually contains an enzyme-linked plate and reagent bottles together in a box body, if the reagent bottles are damaged and polluted, the enzyme-linked plate can be influenced at the same time, or areas for containing the enzyme-linked plate and auxiliary tools are not arranged in the box, so that the kit is only used for containing the reagent bottles, and the enzyme-linked plate is inconvenient to take or corresponding tools are additionally required to be carried when the kit is used; meanwhile, the kit is not strong in universality and can only be used for detection aiming at a single experiment generally, if multiple detection conditions exist simultaneously, multiple kits and related tools need to be used, the carrying process of a large number of tools and an enzyme label plate is inconvenient, and resources are easy to damage and waste.

SUMMERY OF THE UTILITY MODEL

The utility model provides an enzyme linked immunosorbent assay kit with a new structure, which can effectively solve the defect problem of the traditional kit in the background technology; the kit is used for uniformly accommodating objects such as an ELISA plate, a reagent, appliances and the like, can be conveniently taken, simultaneously ensures the safety of the carrying process and the universality of the using process, and can carry out complete enzyme-linked immunoassay experiments.

The utility model discloses a following technical scheme realizes the purpose:

a kit for enzyme-linked immunosorbent assay comprises a box body and reagent bottles, wherein the upper part of the box body is hinged with a box cover, the bottom surface of the box cover is provided with an embedded groove for embedding a description card, the box body is internally provided with an inner baffle, the inner baffle divides the box body into an appliance area, a reagent bottle area and an auxiliary area from left to right, the reagent bottle area is provided with a plurality of reagent bottles, the bottom of the box body is provided with an enzyme label plate accommodating cavity with an opening at the left side of the box body, the enzyme label plate accommodating cavity is not communicated with the appliance area, the reagent bottle area and the auxiliary area in the box body, the bottom of the opening of the enzyme label plate accommodating cavity is hinged with an accommodating cavity cover, the accommodating cavity cover realizes the opening and closing functions through a buckle switch arranged at the left side of the box body, long-side walls of the enzyme label plate accommodating cavity are provided with guide rails with the same length as the side walls in parallel, the guide rails are connected with slide blocks in a sliding way, the slide block is connected with one end of the spring, the other end of the spring is connected to the side wall of the short side of the elisa plate accommodating cavity, and the elisa plate accommodating cavity is used for accommodating an elisa plate which is matched with the guide rail and provided with a sliding groove.

Furthermore, the cross-section of lid is the L type, and the biggest opening angle of lid is 145 degrees, and the quantity of the bottom surface embedded groove of lid is 2, and the both ends of each embedded groove are provided with a plurality of embedded blocks.

Furthermore, the ELIAS plate is a 96-hole-site ELIAS plate and comprises an ELIAS plate hole frame and ELIAS plate hole strips, letters and digital marks corresponding to the positions of the ELIAS plate holes are arranged around the ELIAS plate, sliding grooves are arranged on the front long side and the rear long side of the ELIAS plate, arc-shaped chamfers are arranged at the bottom of the left short side and the right short side, and the ELIAS plate hole strips are connected with the ELIAS plate hole frame in a clamping mode corresponding to each hole site.

Specifically, the marks arranged in the long side direction of the ELISA plate are marks of numbers 1 to 12, and the marks arranged in the short side direction of the ELISA plate are marks of letters A to H.

Further, a sponge block is arranged at the bottom of the reagent bottle area inside the box body, a plurality of reagent bottle holes are uniformly formed in the sponge block, and reagent bottles are placed in the reagent bottle holes.

Furthermore, a sponge cover plate is arranged above the appliance area, the reagent bottle area and the auxiliary area inside the box body, a positioning groove corresponding to the upper part of the inner partition plate is formed in the bottom of the sponge cover plate, the thickness value of the part, corresponding to the reagent bottle area, of the sponge cover plate is larger than that of the other part, and a plurality of reagent bottle cover holes corresponding to the positions of the reagent bottles are formed in the bottom of the part.

Furthermore, the apparatus area in the box body is provided with a waste liquid tank and a dropper rack, the height of the left side wall of the waste liquid tank is the same as that of the inner partition plate, the upper part of the left side wall is provided with a handle of the waste liquid tank, and the dropper rack is placed above the notch of the waste liquid tank near the left side wall of the waste liquid tank.

Further, the dropper rack comprises a dropper rack supporting column arranged at four corners and an upper layer and a lower layer of placing plates connected with the dropper rack supporting column, the placing plates are provided with placing holes corresponding to a plurality of positions, a dropper rack handle is arranged at the front and rear positions of the upper surface of the upper layer placing plate, and the dropper penetrates through the two layers of placing holes of the placing plates and is placed in the placing holes.

Furthermore, the front part of the box body is also provided with an air vent, and the air vent is arranged at the position of the reagent bottle area inside the box body in a push-pull opening and closing mode.

Furthermore, the bottom surface of box body still is provided with the slipmat that has certain thickness, the slipmat is provided with 4 altogether, is located the position of being close to the four corners department of box body bottom surface respectively.

To sum up, owing to adopted this technical scheme, the beneficial effects of the utility model are as follows:

1. the reagent box of the utility model uniformly stores the ELIAS plate, the reagent bottle, the tools and the like, and all areas in the reagent box are not influenced mutually, the storage of the ELIAS plate is firm and stable, and the ELIAS plate is not easy to be polluted; meanwhile, the operation of taking each article in the reagent box is more convenient, complex steps are avoided, and more articles do not need to be moved; the utility model discloses the whole removal convenience that carries of kit, and the reagent bottle holds the number more, and the apparatus is complete, and consequently the commonality is strong, can accomplish all kinds of ELISA adsorption assay experiments.

2. The elisa plate in the reagent box is matched with the containing cavity in the reagent box, and the taking mode is push-pull sliding, so that convenience is realized; the ELISA plate is fixed in the box body through the cover plate and the guide rail, is safe and stable during movement, and is not easy to damage; the ELISA plate adopts a standard 96-hole ELISA plate, has good experimental performance, and the ELISA hole strip is detachable and can be used and replaced as required.

3. The auxiliary area in the reagent box can store articles such as instruction cards, the instruction cards can be embedded into the box cover, when the reagent box is used, the operation prompt of experimenters can be taken as, meanwhile, the change of the tools and the instruction cards in the tool area can be conveniently carried out according to different experimental contents, and the reagent box is enabled to be stronger in universality.

4. Important appliance droppers required by the kit are placed in the appliance area, and a plurality of droppers can be placed and used corresponding to different reagents, so that the kit can be used for various experimental determinations perfectly; the reagent kit does not need to additionally bring other articles when being carried out, and the waste liquid tank in the appliance area can temporarily store the reagent waste liquid after the dropper is used in the experiment process, so that the outgoing experiment is more efficient and convenient.

5. When the reagent bottle is stored in the reagent box, the reagent bottle is positioned and protected by the upper sponge block and the lower sponge block, so that the reagent bottle cannot shake, and meanwhile, the reagent bottle area is separated from other areas, so that pollution and resource waste caused by damage of the reagent bottle are avoided; reagent storage collocation in the reagent bottle area can be selected according to different experimental contents, and marking indication is carried out on a corresponding instruction card, so that the universality of the kit is further enhanced, and personnel can conveniently use and replace the kit in a unified manner.

6. Before the experiment begins, liquid in the reagent bottle needs appropriate experimental temperature, can put into the constant temperature region with the kit usually, and the blow vent on the box body is opened to the accessible this moment for reagent temperature as early as possible and experimental temperature phase-match have made things convenient for going on of experiment.

7. When this kit used, the ELIAS plate was when carrying out the color reaction, can directly deposit the intracavity that holds of this kit, deposits process safety and stability, and through the cooperation of guide rail with the recess, the ELIAS plate can not become flexible, closes and holds behind the chamber lid, and the box body provides suitable dark surrounds promptly, is favorable to going on of color reaction for the kit is convenient more effective when using outward.

Drawings

FIG. 1 is a schematic structural diagram of the kit;

FIG. 2 is a schematic view from the bottom of the kit;

FIG. 3 is a schematic structural diagram of an ELISA plate;

FIG. 4 is a schematic view of a sponge cover plate;

FIG. 5 is a schematic diagram of a reagent bottle zone;

FIG. 6 is a schematic view of a drop tube region;

FIG. 7 is a schematic diagram showing the nozzle region disassembled;

fig. 8 is a front bottom perspective view of the kit.

The specific meanings represented by the symbols in the drawings are as follows:

1-box body, 11-inner baffle, 12-vent, 13-non-slip mat, 14-elisa plate containing cavity, 2-box cover, 21-embedded groove, 22-embedded block, 31-containing cavity cover, 32-buckle switch, 33-slide block, 34-spring, 35-guide rail, 4-instruction card, 5-elisa plate, 51-elisa hole frame, 52-sliding groove, 53-mark, 54-elisa hole strip, 6-sponge cover plate, 61-positioning groove, 62-reagent bottle cover hole, 7-sponge block, 71-reagent bottle hole, 72-reagent bottle, 8-dropper, 81-waste liquid groove, 82-dropper rack, 83-waste liquid groove handle, 84-dropper rack support, 85-placing plate, 86-placing holes, 87-dropper rack handles.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

Example 1

As shown in fig. 1 and 2, a kit for enzyme-linked immunosorbent assay, includes box body 1 and reagent bottle 72, box body 1 upper portion is articulated to be connected with lid 2, the bottom surface of lid 2 is provided with embedded groove 21 that is used for embedding description card 4, box body 1 is inside to be provided with inner baffle 11, inner baffle 11 divides box body 1 is inside to divide into apparatus district, reagent bottle district and auxiliary area from a left side to the right side, a plurality of reagent bottles 72 have been placed in the reagent bottle district, box body 1 bottom is provided with the opening and is located box body 1 left side ELIAS plate and holds chamber 14, the ELIAS plate holds chamber 14 and box body 1 inside apparatus district, reagent bottle district and auxiliary area and do not communicate.

As shown in fig. 5 and fig. 8, the articulated chamber lid 31 that holds that is connected with in the opening part bottom of ELIAS plate holding chamber 14, hold the chamber lid 31 and realize the switching function through setting up in the left buckle switch 32 of box body 1, the ELIAS plate holds parallel arrangement on the long limit lateral wall of chamber 14 and has the isometric guide rail 35 of lateral wall, sliding connection has slider 33 on the guide rail 35, slider 33 is connected with the one end of spring 34, the other end of spring 34 is connected on the minor face lateral wall of ELIAS plate holding chamber 14, ELIAS plate holding chamber 14 is used for holding have with guide rail 35 assorted have the ELIAS plate 5 of slip recess 52.

As shown in fig. 1 and 2, the section of the box cover 2 of the reagent box is L-shaped, the maximum opening angle of the box cover 2 is 145 degrees, the number of the bottom surface insertion grooves 21 of the box cover 2 is 2, and a plurality of insertion blocks 22 are arranged at two ends of each insertion groove 21.

In this embodiment, the inside of the reagent kit has 4 mutually separated accommodating spaces, namely: an appliance area, a reagent bottle area, an auxiliary area and an enzyme label plate accommodating cavity 14; some tools for enzyme-linked immunoassay are placed in the tool area, various reagents are placed in the reagent bottle area, instruction cards 4 and other auxiliary objects for various experiments which can be carried out by the kit can be placed in the auxiliary area, and the elisa plate 5 is placed in the elisa plate accommodating cavity 14.

In the elisa plate accommodating cavity 14, the sliding block 33 and the spring 34 are devices which enable the elisa plate 5 to be conveniently drawn out and fixed, and the two sides of the sliding block 33 are also provided with grooves matched with the guide rail 35, so that the sliding block can slide on the guide rail 35 and is connected with the spring 34; when putting into ELIAS plate 5, ELIAS plate 5 is through its cooperation of sliding groove 52 and guide rail 35, slide in the ELIAS plate holds the chamber 14, can contact slider 33, along with the income of ELIAS plate 5, compression spring 34 when slider 33 receives the contact promotion of ELIAS plate 5, when ELIAS plate 5 holds into the ELIAS plate completely and holds chamber 14, spring 34 is also in the state of compressed, close this moment and hold chamber lid 31, ELIAS plate 5 is promptly by the guide rail, slider 33 and hold chamber lid 31 and restrict jointly and fix, can not rock.

The accommodating cavity cover 31 realizes the opening and closing function through a buckle switch 32 arranged on the left side of the box body 1, the buckle switch 32 belongs to the prior art, detailed description is omitted, and personnel can lock the accommodating cavity cover 31 by directly closing the accommodating cavity cover; when the ELISA plate is opened, the switch buckle is only required to be moved, the containing cavity cover 31 is opened, and the ELISA plate 5 can be pushed out of the cavity opening under the action of the spring 34 in a compressed state and the connected slide block 33, so that the taking of the ELISA plate by personnel is facilitated.

In this embodiment, the maximum opening angle of the box cover 2 is 145 degrees, which facilitates the reading of the instruction card 4 set therein by a person when opening, and the instruction card 4 is inserted through the insertion blocks 22 at the two ends of the bottom surface insertion groove 21 of the box cover 2, and different instruction cards 4 can be conveniently replaced.

When the kit is used, the kit is properly placed on the right hand side of a personnel operating table, the box cover 2 is opened to take the contents and read the instruction card 4, the elisa plate 5 is taken through the opening of the elisa plate accommodating cavity 14 at the bottom of the left side, and the whole using process is more convenient; when the ELISA plate 5 needs to be developed after being used, the ELISA plate 5 is placed back into the ELISA plate accommodating cavity 14, the kit provides a black environment for the ELISA plate 5, and the stability and safety of the detection of the developing condition by drawing the ELISA plate 5 in are ensured.

Example 2

On the basis of embodiment 1, as shown in fig. 3, the elisa plate 5 is a 96-hole elisa plate, and comprises an elisa hole frame 51 and elisa hole strips 54, the periphery of the elisa plate 5 is provided with letters and digital marks 53 corresponding to the positions of the elisa holes, the front and rear long side surfaces of the elisa plate 5 are provided with sliding grooves 52, the bottom of the left and right short side surfaces are provided with circular arc chamfers, and the elisa hole strips 54 are connected with each hole position corresponding to the elisa hole frame 51 in a clamping manner.

Specifically, the marks arranged in the long side direction of the microplate 5 are marks of numbers 1 to 12, and the marks arranged in the short side direction of the microplate 5 are marks of letters a to H.

The 96-hole ELISA plate is an ELISA plate with the most suitable universal enzyme-linked immunosorbent assay plate hole number in the enzyme-linked immunosorbent assay, and can complete almost all enzyme-linked immunosorbent assays; simultaneously, the structural design of the ELISA plate 5 provided by the embodiment is matched with the kit, the use is more convenient, and the arc-shaped chamfer at the bottom of the ELISA plate ensures that the contact between the ELISA plate 5 and the accommodating cavity cover 31 is smoother.

Enzyme-labeled hole strip 54 on enzyme-labeled plate 5 in this embodiment adopts removable design, can how much according to the experiment content to choose enzyme-labeled hole strip 54 number, and enzyme-labeled hole strip 54 can use most similar enzyme-labeled hole strip 54 on the market, all can the joint install on enzyme-labeled hole frame 51 of enzyme-labeled plate 5 for the compatible ability of this kit is stronger, and the use and the change of enzyme-labeled hole strip 54 are comparatively convenient.

Example 3

On the basis of the embodiment 1, as shown in fig. 4 and 5, a sponge block 7 is arranged at the bottom of the reagent bottle area inside the box body 1, a plurality of reagent bottle holes 71 are uniformly arranged on the sponge block 7, and reagent bottles 72 are placed in the reagent bottle holes 71.

A sponge cover plate 6 is arranged above the tool area, the reagent bottle area and the auxiliary area inside the box body 1, a positioning groove 61 corresponding to the upper part of the inner partition plate 11 is formed in the bottom of the sponge cover plate 6, the thickness value of the part, corresponding to the reagent bottle area, of the sponge cover plate 6 is larger than that of the rest part, and a plurality of reagent bottle cover holes 62 corresponding to the positions of the reagent bottles 72 are formed in the bottom of the part.

When the reagent bottle 72 is stored in the reagent box, the reagent bottle 72 is positioned and protected by the sponge block 71 at the lower part of the reagent bottle, and the sponge cover plate 6 above the reagent bottle is positioned and protected, so that the reagent bottle 72 is positioned in a relatively safe and stable storage and moving environment, and meanwhile, due to the separation of the reagent bottle area and other areas and the capping of the sponge cover plate 6, the reagent bottle is not easy to pollute other areas even if being damaged, and the safety of the ELISA plate 5 can not be influenced.

The sponge block 7 and the sponge cover plate 6 used in cooperation with the reagent bottle area provided by the embodiment can position and contain 16 reagent bottles 72, and contain the reagent bottles in 4 rows and 4 columns in a square form, so that the reagent types of the reagent bottles 72 at all positions can be recorded in the card according to different experiment types.

When a person opens the box cover 2, the sponge cover plate 6 can see the upper part of the area in the box, and the article can be conveniently taken out through the depressions at the two ends of the sponge cover plate 6, so that the articles in the appliance area, the reagent bottle area and the auxiliary area can be obtained; simultaneously just because the existence of sponge apron 6, the article in apparatus district, reagent bottle district and the auxiliary area are all fixed, and originally there is the space above three region to be filled for article can not rock the pine and take off when removing and carrying, and is safer.

Example 4

In addition to example 3, as shown in FIGS. 6 and 7, a waste liquid tank 81 and a dropper rack 82 are provided in the tool area in the housing 1, the waste liquid tank 81 has a left side wall having the same height as that of the internal partition 11, a handle 83 for the waste liquid tank is provided on the upper portion of the left side wall, and the dropper rack 82 is placed above the notch of the waste liquid tank 81 in close proximity to the left side wall of the waste liquid tank 81.

The dropper rack 82 comprises a dropper rack support 84 arranged at four corners and an upper layer and a lower layer of placing plates 85 connected with the dropper rack support, a plurality of placing holes 86 corresponding to each other are arranged on the placing plates 85, dropper rack handles 87 are arranged at the front and rear positions of the upper surface of the upper layer of placing plates 85, and droppers 8 are placed in the placing holes 86 of the two layers of placing plates 85.

The dropper 8 is used for sucking the reagent in the reagent bottle 72 and is an important tool in the experiment, and the embodiment is designed for storing the dropper 8 in the tool area in the reagent box; the dropper 8 is normally placed on the dropper rack 82 with its opening facing downward, and is fixed by the dropper rack 82 and the sponge cover plate 6 on the top.

A plurality of droppers can be arranged on the dropper rack 82, 6 droppers 8 are arranged on the dropper rack 82 provided by the embodiment, and the orifice of each dropper 8 is positioned above the waste liquid tank 81; the waste liquid tank 81 is provided so that when the used dropper 8 is used or cannot be cleaned when the kit is carried out for an experiment, water or liquid thereof naturally drops into the waste liquid tank 81 after the dropper 8 is returned to the dropper rack 82, thereby facilitating the subsequent treatment.

Personnel can conveniently take the dropper out of the tool area in the reagent box for cleaning or replacement through the dropper rack handle 87 on the dropper rack 82; after the dropper rack 82 is taken out, personnel can lift the waste liquid tank 81 by the waste liquid tank handle 83 to clean and treat waste liquid, and after the treatment is finished, the waste liquid tank 81 and the dropper rack 82 are sequentially put back into the kit.

Example 5

In addition to embodiment 1, as shown in fig. 1 and 2, a vent hole 12 is further opened in the front portion of the case 1, and the vent hole 12 is opened and closed by pushing and pulling and is provided at the position of the reagent bottle region inside the case 1.

The bottom surface of the box body 1 is also provided with 4 non-slip mats 13 with certain thickness, and the non-slip mats 13 are respectively positioned at the positions, close to the four corners, of the bottom surface of the box body 1.

When the vent 12 is closed, the space in the box can be ensured to be in a closed state, and the reagent is suitable for being stored in a sealed mode; before carrying out the experiment, because reagent needs suitable experimental temperature, put the kit in the constant temperature region back, can open air vent 12 for the inside and outside circulation of air of box body 1, reagent temperature can change the experimental temperature to the constant temperature region relatively fast, has made things convenient for the high efficiency of experiment to go on.

The setting up of slipmat 13 makes box body 1 when guaranteeing to place stably, has raised the take height relatively for the opening angle that holds chamber lid 31 of left side bottom is bigger, and the setting of cooperation buckle switch 32 makes taking and putting back of ELIAS plate 5 more convenient.

Claims (10)

1. A kit for enzyme-linked immunosorbent assay, which comprises a kit body (1) and a reagent bottle (72), and is characterized in that: the reagent box is characterized in that the upper part of the box body (1) is hinged and connected with a box cover (2), the bottom surface of the box cover (2) is provided with an embedded groove (21) for embedding a description card (4), an inner baffle (11) is arranged inside the box body (1), the inner baffle (11) divides the inside of the box body (1) into an appliance area, a reagent bottle area and an auxiliary area from left to right, a plurality of reagent bottles (72) are placed in the reagent bottle area, the bottom of the box body (1) is provided with an ELISA plate accommodating cavity (14) with an opening positioned on the left side of the box body (1), the ELISA plate accommodating cavity (14) is not communicated with the appliance area, the reagent bottle area and the auxiliary area inside the box body (1), the bottom of the opening of the ELISA plate accommodating cavity (14) is hinged and connected with an accommodating cavity cover (31), and the accommodating cavity cover (31) realizes the opening and closing functions through a buckle switch (32) arranged on the left side of the box body (1), the ELIAS plate holds on the long limit lateral wall of chamber (14) parallel arrangement have with the isometric guide rail (35) of lateral wall, sliding connection has slider (33) on guide rail (35), slider (33) are connected with the one end of spring (34), the other end of spring (34) is connected on the short side lateral wall that the ELIAS plate held chamber (14), the ELIAS plate holds chamber (14) and is used for holding have with guide rail (35) assorted ELIAS plate (5) that have slip recess (52).

2. The kit for enzyme-linked immunosorbent assay according to claim 1, wherein: the cross-section of lid (2) is the L type, and the biggest opening angle of lid (2) is 145 degrees, and the quantity of the bottom surface embedded groove (21) of lid (2) is 2, and the both ends of each embedded groove (21) are provided with a plurality of embedding pieces (22).

3. The kit for enzyme-linked immunosorbent assay according to claim 1, wherein: the ELISA plate (5) is a 96-hole ELISA plate and comprises an ELISA hole frame (51) and ELISA hole strips (54), letters and digital marks (53) corresponding to the positions of the ELISA holes are arranged around the ELISA plate (5), sliding grooves (52) are arranged on the side faces of the front long edge and the rear long edge of the ELISA plate (5), arc-shaped chamfers are arranged at the bottoms of the side faces of the left short edge and the right short edge, and the ELISA hole strips (54) are connected with the ELISA hole frame (51) in a clamping mode corresponding to each hole position.

4. The kit for enzyme-linked immunosorbent assay according to claim 3, wherein: the label (53) arranged in the long side direction of the ELISA plate (5) is a label with the number from 1 to 12, and the label (53) arranged in the short side direction of the ELISA plate (5) is a label with the letter from A to H.

5. The kit for enzyme-linked immunosorbent assay according to claim 1, wherein: the reagent bottle box is characterized in that a sponge block (7) is arranged at the bottom of a reagent bottle area in the box body (1), a plurality of reagent bottle holes (71) are uniformly formed in the sponge block (7), and reagent bottles (72) are placed in the reagent bottle holes (71).

6. The kit for enzyme-linked immunosorbent assay according to claim 1, wherein: a sponge cover plate (6) is arranged above the appliance area, the reagent bottle area and the auxiliary area inside the box body (1), a positioning groove (61) corresponding to the upper portion of the inner partition plate (11) is formed in the bottom of the sponge cover plate (6), the thickness value of the part, corresponding to the reagent bottle area, of the sponge cover plate (6) is larger than that of the rest part, and a plurality of reagent bottle cover holes (62) corresponding to the positions of the reagent bottles (72) are formed in the bottom of the part.

7. The kit for enzyme-linked immunosorbent assay according to claim 1, wherein: the utensil district in box body (1) is provided with waste liquid groove (81) and dropper frame (82), the left side lateral wall height of waste liquid groove (81) is the same with the height of interior baffle (11), and left side lateral wall upper portion is provided with waste liquid groove handle (83), dropper frame (82) are placed in waste liquid groove (81) notch top near the left side lateral wall of waste liquid groove (81).

8. The kit of claim 7, wherein the kit comprises: the dropper rack (82) comprises dropper rack supporting columns (84) arranged at four corners and upper and lower placing plates (85) connected with the dropper rack supporting columns, a plurality of placing holes (86) corresponding to the positions are formed in the placing plates (85), dropper rack handles (87) are arranged in the front and rear positions of the upper surface of the upper placing plate (85), and droppers (8) penetrate through the placing holes (86) of the two placing plates (85) and are placed in the dropper rack lifting handles.

9. The kit for enzyme-linked immunosorbent assay according to claim 1, wherein: the front part of the box body (1) is also provided with an air vent (12), and the air vent (12) is arranged at the position of a reagent bottle region in the box body (1) in a push-pull opening and closing mode.

10. The kit of claim 1, wherein the kit comprises: the bottom surface of the box body (1) is also provided with 4 non-slip pads (13) with certain thickness, and the non-slip pads (13) are respectively positioned at the positions, close to the four corners, of the bottom surface of the box body (1).

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