CN217077617U - Temperature changing device suitable for medical fluorescent quantitative PCR - Google Patents
Temperature changing device suitable for medical fluorescent quantitative PCR Download PDFInfo
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- CN217077617U CN217077617U CN202123331201.4U CN202123331201U CN217077617U CN 217077617 U CN217077617 U CN 217077617U CN 202123331201 U CN202123331201 U CN 202123331201U CN 217077617 U CN217077617 U CN 217077617U
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Abstract
The utility model discloses a temperature changing device suitable for medical fluorescence quantitative PCR, belong to fluorescence quantitative PCR instrument technical field, including test tube seat and radiator, radiator upper portion installs at least one TEC cold and hot piece, and the TEC cold and hot piece outside is provided with the heat preservation cotton, and the test tube seat is installed on TEC cold and hot piece, and the radiator lower part is installed the wind channel, and the wind channel below is connected with the fan, installs first temperature sensor on the test tube seat, installs on the radiator and has second temperature sensor, and the structure of temperature changing module has been simplified to this device, has improved the control by temperature change precision of lift temperature module simultaneously, control by temperature change homogeneity, the speed of rising and falling temperature; the temperature changing module can accurately realize high-temperature denaturation, low-temperature renaturation and thermal cycle of proper temperature extension, and meets the requirement of a PCR instrument on temperature control.
Description
Technical Field
The utility model belongs to the technical field of fluorescence quantitative PCR instrument, a alternating temperature device suitable for medical fluorescence quantitative PCR is related to.
Background
The fluorescent quantitative PCR is a method for measuring the total amount of products after each polymerase chain reaction cycle by using fluorescent chemical substances in DNA amplification reaction, and a method for quantitatively analyzing a specific DNA sequence in a sample to be detected by an internal reference method or an external reference method. Mainly applied to the fields of medical clinical disease diagnosis (hepatitis, AIDS, combination and the like), animal disease detection (avian influenza, swine fever and the like), food safety (food allergen, transgene and the like), scientific research and the like; the PCR temperature control part on the market has a complex structure, and particularly has low precision in the temperature control uniformity part, so that the requirements of increasingly high precision and high reliability in medical inspection cannot be met.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is not enough to above-mentioned prior art, and provide a temperature varying device suitable for medical fluorescence quantitative PCR, this a temperature varying device suitable for medical fluorescence quantitative PCR is for solving above-mentioned technical problem, the utility model provides a following technical scheme: the utility model provides a alternating temperature device suitable for medical fluorescence quantitative PCR, includes test tube seat and radiator, at least one TEC cold and hot piece is installed on radiator upper portion, the TEC cold and hot piece outside is provided with the heat preservation cotton, the test tube seat is installed on the TEC cold and hot piece, the wind channel is installed to the radiator lower part, the wind channel below is connected with the fan, install first temperature sensor on the test tube seat, it has second temperature sensor to install on the radiator.
Preferably, the temperature sensor is PT 1000.
Preferably, the second temperature sensor is an NTC temperature sensor.
Preferably, an upper cover is arranged above the test tube seat and the TEC cold and hot plate.
Preferably, 4 TEC cold and hot plates are mounted on the upper portion of the heat radiator.
Preferably, the number of the test tube holes on the test tube seat is 32.
Has the advantages that: the device simplifies the structure of the temperature changing module, and simultaneously improves the temperature control precision, the temperature control uniformity and the temperature increasing and decreasing speed of the temperature increasing and decreasing module; the temperature changing module can accurately realize high-temperature denaturation, low-temperature renaturation and thermal cycle of proper temperature extension, and meets the requirement of a PCR instrument on temperature control.
Drawings
Fig. 1 is a schematic top view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a sectional view taken along line B-B;
the symbols in the drawings illustrate that: 1: a test tube seat; 2: an upper cover; 3: a heat sink; 4: an air duct; 5: a fan; 6: a first temperature sensor; 7: a TEC cold and hot plate; 8: heat preservation cotton; 9: a second temperature sensor; 10: test tube hole.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific preferred embodiments.
In the description of the present invention, it should be understood that the terms "left side", "right side", "upper part", "lower part" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for convenience of description and simplification of description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, "first", "second" and the like do not indicate the degree of importance of the component parts, and thus, are not to be construed as limiting the present invention. The specific dimensions used in the present embodiment are only for illustrating the technical solution, and do not limit the protection scope of the present invention.
Example 1:
referring to fig. 1-3, the utility model provides a technical scheme, a alternating temperature device suitable for medical fluorescence quantitative PCR, including test tube seat 1 and radiator 3, at least one TEC cold and hot piece 7 is installed on 3 upper portions of radiator, and the TEC cold and hot piece 7 outside is provided with heat preservation cotton 8, and test tube seat 1 is installed on TEC cold and hot piece 7, and wind channel 4 is installed to 3 lower parts of radiator, and wind channel 4 below is connected with fan 5, installs first temperature sensor 6 on the test tube seat 1, installs on the radiator 3 to have second temperature sensor 9.
The heat radiator 3 is used for quickly dissipating redundant heat inside the structure when the temperature rises or falls, the heat stability of the device is kept, the TEC cold and hot fins 7 are installed on the heat radiator 3, the TEC cold and hot fins 7 serve as a heat source and a cold source of the structure, the structure has the advantages that one end is used for refrigerating, the other end is used for heating, the thermal inertia is very small, the refrigerating and heating are very quick, the heat insulation cotton 8 is arranged around the TEC cold and hot fins 7 and used for preventing the heat from being dissipated outwards, the effect of low thermal edge is reduced, the temperature uniformity among holes of the test tube base 1 is further improved, and the requirement of PCR on the temperature control uniformity is met; the test tube seat 1 is used for receiving heat or cold of the TEC, the first temperature sensor 6 is used for monitoring the temperature of the test tube seat 1 in real time and transmitting the temperature back to the terminal control part, then the TEC cold and hot fins 7 are controlled to heat or cool according to a real-time feedback result of the first temperature sensor 6 so as to meet the temperature setting requirement, the air duct 4 can fix the direction of air blown out by the fan 5, the radiator 3 is enabled to be uniform in wind direction, hot air is blown out of the instrument, the second temperature sensor 9 monitors the temperature of the loading platform of the radiator 3 in real time so as to control the air outlet size of the fan 5 in a feedback mode, the heat concentrated by the radiator 3 can be timely dissipated, and the test tube seat 1 is heated evenly.
Further, the first temperature sensor 6 is of the type PT 1000.
Further, the second temperature sensor 9 is an NTC temperature sensor.
Further, an upper cover 2 is arranged above the test tube seat 1 and the TEC cold and hot plate 7, the part 2 is an upper cover and is used for covering the test tube seat, the TEC heat preservation device and the like,
furthermore, 4 TEC cold-hot plates 7 are installed on the upper portion of the radiator 3, and the error of the resistance value of the TEC cold-hot plates 7 can be effectively reduced by independently controlling the 4 TEC cold-hot plates 7, so that the test tube seat 1 is unevenly heated.
Further, the number of the test tube wells 10 on the test tube holder 1 is 32.
Example 2:
on the basis of embodiment 1, the device is installed on a PCR instrument, a power supply is switched on, corresponding connecting lines of the TEC cold and hot plates 7, connecting lines of the first temperature sensor 6 and the second temperature sensor 9, and a fan 5 are connected with corresponding circuit board positions through wires, instructions are sent through software control, the TEC cold and hot plates 7 work, the temperature of the test tube seat 1 is firstly raised to 55 ℃, when the first temperature sensor 6 monitors the temperature to 55 ℃, the TEC cold and hot plates 7 stop heating, the temperature is controlled to 55 ℃ for 2 minutes, the software instructions raise the temperature of the test tube seat 1 to 95 ℃ and control the temperature for 2 minutes, the upper hot surface of the TEC cold and hot plates 7 heats, when the first temperature sensor 6 monitors the temperature to 95 ℃, the TEC cold and hot plates 7 stop heating, the temperature is controlled to 95 ℃ for 2 minutes, the software instructions lower the test tube seat 1 to 55 ℃ and control the temperature for 2 minutes, at the moment, the cold and hot surfaces of the TEC cold and hot plates 7 are switched, the upper surface in contact with the test tube seat 1 is a cold surface, the test tube seat 1 is cooled, the upper surface in contact with the radiator 3 is a hot surface, heat is radiated by the radiator 3, the fan 5 radiates the radiator 3 through the air duct 4, when the temperature of the test tube seat is monitored by the first temperature sensor 6 to 55 ℃, the TEC cold and hot plates 7 stop refrigerating, the temperature lasts for 2 minutes, and the temperature control is a cold and hot circulation. In specific work, a plurality of cold and hot cycles can be realized through software and circuit control according to the requirement of the reagent on the temperature.
While the present invention has been described in detail with reference to the preferred embodiments thereof, it should be understood that the above description should not be taken as limiting the present invention. Numerous modifications and alterations to the present invention will become apparent to those skilled in the art upon reading the foregoing description. The technical idea of the utility model within the scope, can be right the utility model discloses a technical scheme carries out multiple transform of equaling, and these transform of equaling all belong to the utility model discloses a protection scope.
Claims (6)
1. The utility model provides a alternating temperature device suitable for medical fluorescence quantitative PCR which characterized in that: including test tube seat (1) and radiator (3), at least one TEC cold and hot piece (7) is installed on radiator (3) upper portion, the TEC cold and hot piece (7) outside is provided with heat preservation cotton (8), install on TEC cold and hot piece (7) test tube seat (1), wind channel (4) are installed to radiator (3) lower part, wind channel (4) below is connected with fan (5), install first temperature sensor (6) on test tube seat (1), install on radiator (3) and have second temperature sensor (9).
2. The temperature-changing device suitable for medical fluorescent quantitative PCR according to claim 1, wherein: the type of the temperature sensor (6) is PT 1000.
3. The temperature-changing device suitable for medical fluorescent quantitative PCR according to claim 1, wherein: the second temperature sensor (9) is an NTC temperature sensor.
4. The temperature-changing device suitable for medical fluorescent quantitative PCR according to claim 1, wherein: an upper cover (2) is arranged above the test tube seat (1) and the TEC cold and hot plate (7).
5. The temperature-changing device suitable for medical fluorescent quantitative PCR according to claim 1, wherein: 4 TEC cold and hot plates (7) are installed on the upper portion of the radiator (3).
6. The temperature-changing device suitable for medical fluorescent quantitative PCR according to claim 1, wherein: the number of the test tube holes (10) on the test tube seats (1) is 32.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123331201.4U CN217077617U (en) | 2021-12-28 | 2021-12-28 | Temperature changing device suitable for medical fluorescent quantitative PCR |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123331201.4U CN217077617U (en) | 2021-12-28 | 2021-12-28 | Temperature changing device suitable for medical fluorescent quantitative PCR |
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| Publication Number | Publication Date |
|---|---|
| CN217077617U true CN217077617U (en) | 2022-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202123331201.4U Active CN217077617U (en) | 2021-12-28 | 2021-12-28 | Temperature changing device suitable for medical fluorescent quantitative PCR |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117286025A (en) * | 2023-09-13 | 2023-12-26 | 艾普拜生物科技(苏州)有限公司 | Temperature control unit for PCR instrument |
-
2021
- 2021-12-28 CN CN202123331201.4U patent/CN217077617U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117286025A (en) * | 2023-09-13 | 2023-12-26 | 艾普拜生物科技(苏州)有限公司 | Temperature control unit for PCR instrument |
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