CN217059606U - Positioning brain tissue embedding mould - Google Patents
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- CN217059606U CN217059606U CN202122898806.5U CN202122898806U CN217059606U CN 217059606 U CN217059606 U CN 217059606U CN 202122898806 U CN202122898806 U CN 202122898806U CN 217059606 U CN217059606 U CN 217059606U
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Abstract
The application relates to a positioning brain tissue embedding mold. This mould includes the recess, and the recess includes the bottom, lateral wall, opening and outer edge, and the bottom sets up first horizontal line and first vertical line towards open-ended one side, and first horizontal line and first vertical line intersect perpendicularly, and the nodical is first nodical, sets up first scale mark and second scale mark on the outer edge, and first scale mark is directly over first horizontal line, and the second scale mark is directly over first vertical line, and the extension line of first scale mark and second scale mark intersects perpendicularly. Through set up outer edge on brain tissue embedding mould, set up first scale mark and second scale mark on outer edge, set up first horizontal line and first vertical line at the recess, reference standard when confirming the embedding brain tissue, the embedding in-process of being convenient for maintains brain tissue bilateral symmetry, the operation of brain tissue ventral and dorsal part vertical form about, the accuracy of section direction after guaranteeing the embedding improves the accuracy of target area section direction and the accuracy that target area protein or RNA expression condition judge.
Description
Technical Field
The application relates to an embedding mould of biological tissue, in particular to a positioning brain tissue embedding mould.
Background
In nervous system studies, immunohistochemical staining of brain tissue is often required. The most commonly used method is to slice the brain tissue with fixed shape, then to mark the protein or RNA (ribosomal acid) on the brain tissue slice with antibody or probe, and to present the different protein or RNA expression conditions of different brain areas by fluorescence or tissue staining. The fixed brain tissue form is beneficial to judging the position of each region of the brain tissue, and is beneficial to acquiring the brain tissue of a target region along an accurate direction in the slicing process, thereby acquiring the expression condition of protein or RNA of the target region. In the brain spectrogram, the brain tissue morphology of a mouse in the brain spectrogram is a two-dimensional diagram which is symmetrical about the brain and vertically determined on the ventral side and the dorsal side of the brain tissue. The brain tissue in the mould is corresponding to the brain tissue in the brain spectrogram, so that different areas of the brain tissue can be conveniently identified, marked and sliced. At present, two common ways to fix brain tissue morphology are full-embedding and half-embedding. 1) Full embedding: the brain tissue is fixed by using a cylindrical tin foil groove or a non-fixed shape disposable embedding tool made of other materials. Specifically, the brain tissue is placed in a tin paper tank or other tools with the cerebellum facing downwards and the olfactory bulbs facing upwards, and then an OCT (optical coherence tomography) embedding medium is added to completely wrap the whole brain tissue. 2) Half embedding: the lower half of the brain tissue was fixed to a cryosection holder using OCT.
The two embedding methods have no reference object in the embedding process and completely adjust the shape of the brain tissue by naked eyes, so that an operator can not accurately acquire a target area by slicing the brain tissue in a vertically vertical shape on the ventral side and the dorsal side of the brain tissue instead of the left-right brain symmetry. The deviation between the obtained brain tissue region and the target region influences the accuracy of judging the protein or RNA expression condition of the target region.
Therefore, there is a need for a positioning brain tissue embedding mold, which ensures that an operator performs slicing operation in a state that the left and right brains of the brain tissue are symmetrical and the ventral side and the dorsal side of the brain tissue are vertical, and improves the accuracy of the slicing direction of a target region and the accuracy of judgment of the protein or RNA expression condition of the target region.
SUMMERY OF THE UTILITY MODEL
The embodiments of the present application provide a brain tissue embedding mold, which can solve the problems that an operator can not accurately obtain a target region by slicing the brain tissue in a vertically vertical state on the ventral side and the dorsal side of the brain tissue rather than on the left and right brain symmetry state, and the accuracy of protein or RNA expression judgment is affected by the deviation between the obtained brain tissue region and the target region. The technical scheme is as follows:
the brain tissue embedding mold comprises a groove, wherein the groove comprises a bottom, a side wall, an opening and an outer edge. Wherein, the circumference of bottom upwards extends and forms the lateral wall, the top of lateral wall forms the opening, the opening is to extending all around and form outer edge, the bottom orientation open-ended one side sets up first horizontal line and first vertical line, first horizontal line with the perpendicular crossing of first vertical line, the nodical first nodical, set up first scale mark and second scale mark on the outer edge, first scale mark is in directly over first horizontal line, the second scale mark is in directly over first vertical line, first scale mark and second scale mark's extension line is crossing perpendicularly.
Furthermore, the groove further comprises a plurality of second transverse lines and second longitudinal lines, the second transverse lines are parallel to the first transverse lines, the second longitudinal lines are parallel to the first longitudinal lines, and the first transverse lines or the second transverse lines are crossed with the first longitudinal lines or the second longitudinal lines to form a reticular grid.
Further, the mesh grid has a size of 2mm by 2 mm.
Further, the outer edge comprises a plurality of third scale marks and fourth scale marks, the third scale marks are parallel to the first scale marks, the fourth scale marks are parallel to the second scale marks, and the distance between the third scale marks and the first scale marks is equal to the distance between the fourth scale marks and the second scale marks.
Preferably, the distance between the third graduation mark and the first graduation mark and the distance between the fourth graduation mark and the second graduation mark are 0.05 cm.
Further, still include the drawing of patterns assistor, the drawing of patterns assistor include the bottom piece and with the side piece that the bottom piece is connected, the bottom piece set up in the bottom orientation one side of opening, the side piece is followed the lateral wall sets up.
Furthermore, the demolding assistor further comprises an extrusion sheet, wherein the extrusion sheet is erected above the outer edge and is connected with the side sheet.
Further, still include the stand, the stand set up in the outer edge is kept away from opening one side.
Further, the plane of the outer edge is parallel to the plane of the bottom.
Further, the height of the upright is equal to the depth of the groove.
The utility model provides a beneficial effect that technical scheme brought is through setting up outer edge on brain tissue embedding mould, set up first scale mark and second scale mark on outer edge, set up first horizontal line and first vertical line at the recess, reference standard when confirming the embedding brain tissue, be convenient for the embedding in-process maintain brain tissue bilateral symmetry, the operation of brain tissue ventral side and dorsal part vertically form from top to bottom, guarantee the accuracy of section direction after the embedding, improve the accuracy of target area section direction and the accuracy that target area protein or RNA expression condition were judged.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings used in the description of the embodiments of the present application will be briefly described below.
Fig. 1 is an exploded view of a brain tissue embedding mold according to an embodiment;
fig. 2 is an exploded schematic view of the brain tissue embedding mold provided in the second embodiment.
Detailed Description
In order to make the technical problem, technical scheme and the beneficial effect that the utility model discloses solve clearer, it is right to combine figure and embodiment below to go on further the detailed description of the utility model. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.
The utility model provides a brain tissue embedding mould has the function of the different regions of location brain tissue, specifically, brain tissue embedding mould divides and has four regions, first quadrant, second quadrant, third quadrant and fourth quadrant promptly. The brain tissue is placed in a brain tissue embedding mold, and different areas of the brain tissue correspond to different brain tissue embedding mold areas. The brain tissue is fixed in a brain tissue embedding mould according to the position of the brain tissue in the brain spectrogram, is marked according to the brain spectrogram, and is further sliced in different areas of the brain tissue according to the marks after the OCT is frozen, so that the accuracy of the slicing direction of the target area is ensured, the slicing efficiency is improved, and the accuracy of the slicing direction of the target area and the accuracy of the judgment of the protein or RNA expression condition of the target area are improved.
Example one
Referring to the attached fig. 1 of the specification, the brain tissue embedding mold 100 includes a groove 11, an outer rim 12, a pillar 13 and a demolding aid 14.
The groove 11 is used for placing brain tissue and the embedding agent OCT, and the groove 11 comprises a bottom 111, an opening 112 and a side wall 113. The bottom 111 extends circumferentially upward to form a sidewall 113. The top of the sidewall 113 forms an opening 112, and the opening 112 extends around to form an outer edge 12.
In one embodiment, the bottom, opening, and sidewalls are all rectangular. In another embodiment, the bottom, opening and side walls are all square. Alternatively, the bottom and opening are circular and the side walls are closed rectangles. Alternatively, the bottom and opening are circular and the side wall is a closed sector ring. The side wall and the bottom in the above embodiments may be connected at any one of an acute angle, a right angle and an obtuse angle.
The brain tissue is disposed in the recess 11, and when the operator looks from the opening 112 to the bottom 111 and the brain tissue, and the line of sight is perpendicular to the bottom 111, the median brain seam that divides the brain tissue into the left and right brains can be regarded as a straight line disposed in a plane parallel to the bottom 111, and is referred to as a median brain seam line in this application.
Groove 11 also includes a first transverse line 114 and a first longitudinal line 115 disposed at base 111, where first transverse line 114 and first longitudinal line 115 perpendicularly intersect at a first intersection point 116. The first transverse line 114, the first longitudinal line 115 and the first intersection point 116 are all located on the side of the bottom 111 facing the opening 112. In one embodiment, the base is rectangular, preferably the first transverse line is the line connecting the midpoints of the long sides of the rectangle, and the first longitudinal line is the line connecting the centers of the short sides of the rectangle. In another embodiment, the base is circular and the first transverse line and the first longitudinal line are perpendicular diameters. In the above embodiments, the first intersection points are all located at the center of the bottom. When the optic nerve intersection point on the ventral side of the brain tissue and the junction of the cranial coronal suture and the sagittal suture on the dorsal side of the brain tissue are both arranged right above the first intersection point 116, a pore is left between the periphery and the side wall of the brain tissue, and the pore enables the groove to have applicability to brain tissues of different sizes.
The tissues described in the present application are left-right brain symmetric, the vertical form of the ventral and dorsal sides of the brain tissue is the form of the brain tissue when the optic nerve crossing point of the ventral region of the brain tissue and the junction of the cranial coronal suture and the sagittal suture of the dorsal region of the brain tissue are both disposed right above the first crossing point 116, and the straight line of the central brain suture extends along the first transverse line 114 or the first longitudinal line 115.
The outer rim 12 is an annular flat surface parallel to the bottom 11 or a funnel-shaped annular curved surface formed by the opening 112 extending obliquely upward or downward, and includes an outer periphery 122. The upright 13 is connected to the outer rim 12, and has a supporting function in the vertical direction for the outer rim 12, and the upright 13 is located on the side close to the outer periphery 122. In one embodiment, the window and the outer perimeter are both rectangular. In another embodiment, the window and the outer periphery are both circular.
The outer rim 12 further comprises a first tick mark 124 and a second tick mark 125, the extension of the first tick mark 124 and the second tick mark 125 intersecting perpendicularly. The first graduation mark 124 is directly above the first transverse line 114 and the second graduation mark 125 is directly above the first longitudinal line 115. The plane defined by the first tick mark 124 and the first transverse tick mark 114 and the plane defined by the second tick mark 125 and the first longitudinal tick mark 115 intersect perpendicularly and are both perpendicular to the base 111. The brain tissue embedding mold 100 is divided into four regions, i.e., a first quadrant, a second quadrant, a third quadrant, and a fourth quadrant, by a plane defined by the first scale 124 and the first horizontal line 114, a plane defined by the second scale 125, and a plane defined by the first vertical line 115, wherein when the bottom 111 is a rectangle, the upper left is the first quadrant, the upper right is the second quadrant, the lower right is the third quadrant, and the lower left is the fourth quadrant, as viewed from a side of the short side of the rectangle.
When the brain tissue embedding mold 100 is used, a small amount of embedding agent OCT is placed in the groove 11, and the bottom 111 is fully paved and flattened. The dehydrated brain tissue is placed in the groove 11, the ventral region of the brain tissue is completely flatly attached to the embedding medium OCT laid on the bottom 111, and the junction of the optic nerve of the ventral region of the brain tissue and the junction of the coronal suture and the sagittal suture of the skull of the dorsal region of the brain tissue are both arranged right above the first intersection point 116. In one embodiment, the median cerebral suture extends in a horizontal direction along a first transverse line 114. In another embodiment, the midline brain extends in a horizontal direction along a first longitudinal line 115. The mouse brain tissue is placed in the groove 11 according to the operation of the above embodiment, and the fixed form of the mouse brain tissue is consistent with the state of the mouse brain tissue in the brain spectrogram. The brain tissue form of the mouse in the brain spectrogram is a two-dimensional graph determined by taking a straight line of a cerebral suture as a transverse axis and taking a connecting line of a nerve intersection point and a junction of a skull coronal suture and a sagittal suture as a longitudinal axis. The mouse brain tissue can be labeled at the outer edge 12 with reference to the brain region coordinates of the brain map for different regions of the brain tissue. In one embodiment, the mouse brain tissue dorsal hippocampal tissue is located in a region within the first and second quadrants, 1.25mm to 2.5mm down from the junction of the coronal suture and the sagittal suture, from 1140.8-2 mm from the first transverse line, 1153 mm from the first longitudinal line. Filling the groove 11 with the embedding medium OCT for the brain tissue placed in the groove 11, freezing the whole mould in a refrigerator at-80 ℃, and taking out the whole mould after the OCT embedding medium is completely solidified. The brain tissue is marked on the coagulated embedding medium OCT according to the quadrant information, distance information and depth information marked on the outer rim 12. And after marking is finished, taking out a brain tissue embedding block formed by the brain tissue solidified in the embedding medium OCT. According to the mark, the side surface of the brain tissue block close to the cerebellum part is used as the bottom, and the embedding medium OCT is used for horizontally fixing the bottom of the brain tissue on the frozen section tray, so that the target brain tissue area can be quickly and accurately cut according to the mark.
The uprights 13 serve to support the outer rim 12. One end of the upright 13 is connected to the outer rim 12 and is disposed on a side near the outer periphery 122. When the outer edge 12 is a plane parallel to the bottom 111, the extending direction of the upright 13 is perpendicular to the plane of the outer edge 12. The outer edge 12 is supported by the vertical column 13 and is parallel to the horizontal plane, so that the marking on the outer edge 12 is convenient during operation.
In order to facilitate the removal of the solidified brain tissue embedding block from the recess 11, a demolding aid 14 is further provided in this embodiment.
The demolding aid 14 includes a base plate 141 and a side plate 142. The bottom sheet 141 and the side sheet 142 are fixedly connected. The base member 141 is disposed on the bottom portion 111, and the side member 142 extends toward the opening 112 in the direction of the side wall 113. Preferably, the length of the side panel 142 is greater than or equal to the depth of the recess 11. The concave space formed by the bottom plate 141 and the side plate 142 corresponds to the cross-sectional shape of the groove 11, so that the side plate 142 can be pulled up and the brain tissue embedding block can be separated from the groove 11 by applying an upward force to the brain tissue embedding block through the bottom plate 141.
Before laying the embedding agent OCT, the demolding auxiliary device 14 is arranged on the bottom 111 in the groove 11, the side piece 142 is attached to the inner side of the side wall 113, and then the embedding agent OCT is laid. After the embedding agent OCT has spread the recess 11 and frozen, the base plate 141 pulls out the embedded block of brain tissue by lifting up the side plate 142.
Preferably, two side panels 142 are provided at both ends of the bottom panel 141. More convenient for pulling out the brain tissue embedded block by applying force on two sides.
Preferably, the demolding aid 14 further includes a pressing piece 143, and the pressing piece 143 is connected to the other end of the side piece 142. In use, the squeeze tab 143 is positioned above the rim 12. Before the brain tissue embedding block is pulled out, the pressing piece 143 is pushed toward the opening 112, so that the periphery of the brain tissue embedding block is separated from the side wall of the brain tissue embedding mold.
Example two
The brain tissue embedding mold 200 provided in the second embodiment is the same as the brain tissue embedding mold 100 provided in the first embodiment in basic structure, and the differences are:
The grid squares further divide the four regions of the groove 11 precisely. The arrangement of the net-shaped grids is more beneficial to the fixation of the brain tissue morphology. Preferably, the plurality of transverse lines and the plurality of longitudinal lines have the same spacing therebetween, and the size of the formed mesh grid is 2mm × 2 mm. In one embodiment, the median cerebral suture line extends along a first longitudinal line 215 in the horizontal direction, and the intersection of the optic nerve at the ventral region of the brain tissue, where the coronal suture of the skull at the dorsal region of the brain tissue meets the sagittal suture line, in the vertical direction are both disposed directly above the first intersection 216. In the brain spectrogram, the horizontal distance from the olfactory bulb at the forefront of the brain tissue to the optic nerve crossing point is 6mm, which corresponds to that in the brain tissue embedding mold 200, the olfactory bulb at the forefront of the brain tissue is positioned in the third row of mesh-shaped grids away from the first transverse line 214 in the first quadrant and the second quadrant. In the brain spectrogram, the horizontal distance from the optic nerve crossing point to the brain's distal cerebellum is 10mm, which corresponds to the brain's distal cerebellum being located in the fifth square grid from the first transverse line 214 in the third and fourth quadrants in the brain tissue embedding mold 200.
The positions of different areas of the brain tissue in the spectrogram can be quickly corresponding to the positions of the different areas of the brain tissue in the brain tissue embedding mold 200 through the mesh grids, so that the different areas of the brain tissue can be quickly identified and marked.
The outer rim 22 also includes a plurality of third tick marks 228 and fourth tick marks 229. The third tick mark 228 is parallel to the first tick mark 224, the fourth tick mark 229 is parallel to the second tick mark 225, and the separation between the third tick mark 228 and the first tick mark 224 is equal to the separation between the fourth tick mark 229 and the second tick mark 225. The third scale mark and the fourth scale mark are arranged, so that the description of the positions of different areas of the brain tissue can be scaled by using more detailed and accurate scale marks. If no scale mark is set, the direction and distance information at the second intersection point needs to be represented by characters when the region of the brain tissue is marked, and after the scale mark is set, the direction and distance information can be represented by the scale marks which are away from the second intersection point in the two directions of the second intersection point, the third scale mark and the fourth scale mark.
Preferably, the spacing between the third tick mark 228 and the first tick mark 224 and the spacing between the fourth tick mark 229 and the second tick mark 225 is 0.05 cm.
Taking the position of the first scale mark 224 as 0, from the near to the far from the first scale mark, the position represented by the first third scale mark on both sides is 0.1, the position represented by the second third scale mark is 0.2, the position represented by the third scale mark is 0.3, and so on; the position of the second scale mark 225 is 0, from the near to the far away from the second scale mark, the position represented by the first fourth scale mark on the two sides is 0.1, the position represented by the second fourth scale mark is 0.2, the position represented by the third fourth scale mark is 0.3, and so on … …, thereby different positions in the four areas of the brain tissue embedding mold can be represented by coordinates formed by the numbers of the third scale mark and the fourth scale mark.
Through set up outer edge on brain tissue embedding mould, set up first scale mark and second scale mark on outer edge, set up first horizontal line and first vertical line at the recess, reference standard when confirming the embedding brain tissue, the embedding in-process of being convenient for maintains brain tissue bilateral brain symmetry, the operation of brain tissue ventral side and dorsal side vertical form about, the accuracy of section direction after guaranteeing the embedding improves the accuracy of target region section direction and the accuracy that target region protein or RNA expression condition judged.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not intended to limit the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principles of the present invention should be included within the scope of the present invention.
Claims (10)
1. A brain tissue embedding mold comprises a groove, and is characterized in that the groove comprises
The bottom, the circumference of bottom upwards extends and forms the lateral wall, the top of lateral wall forms the opening, the opening is to extending all around and form outer edge, outer edge includes the periphery, the bottom orientation open-ended one side sets up first horizontal line and first vertical line, first horizontal line with the first vertical line's perpendicular crossing, the nodical is first nodical, outer edge sets up first scale mark and second scale mark, first scale mark is in directly over the first horizontal line, the second scale mark is in directly over the first vertical line, first scale mark and second the extension line of second scale mark is perpendicular crossing.
2. The brain tissue embedding mold of claim 1, wherein the groove further comprises a plurality of second transverse lines and second longitudinal lines, the second transverse lines are parallel to the first transverse lines, the second longitudinal lines are parallel to the first longitudinal lines, and the first transverse lines or the second transverse lines cross the first longitudinal lines or the second longitudinal lines to form a grid-like grid.
3. The brain tissue embedding mold of claim 2, wherein the mesh grid has a size of 2mm x 2 mm.
4. The brain tissue embedding mold according to claim 2 or 3, wherein the outer rim comprises a plurality of third graduation lines and fourth graduation lines, the third graduation lines are parallel to the first graduation lines, the fourth graduation lines are parallel to the second graduation lines, and the distance between the third graduation lines and the first graduation lines is equal to the distance between the fourth graduation lines and the second graduation lines.
5. The brain tissue embedding mold according to claim 4, wherein the distance between the third graduation line and the first graduation line and the distance between the fourth graduation line and the second graduation line are 0.05 cm.
6. The brain tissue embedding mold according to claim 1 or 5, further comprising a demolding aid including a bottom plate and a side plate connected to the bottom plate, the bottom plate being disposed on a side of the bottom portion facing the opening, the side plate being disposed along the side wall.
7. The brain tissue embedding mold of claim 6, wherein the demolding aid further comprises a pressing piece, wherein the pressing piece is arranged above the outer edge and connected with the side piece.
8. The brain tissue embedding mold according to claim 1 or 7, further comprising a post disposed on a side of the outer rim away from the opening.
9. The brain tissue embedding mold of claim 8, wherein the plane of the outer rim is parallel to the bottom.
10. The brain tissue embedding mold of claim 9, wherein the height of the post is equal to the depth of the groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122898806.5U CN217059606U (en) | 2021-11-24 | 2021-11-24 | Positioning brain tissue embedding mould |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122898806.5U CN217059606U (en) | 2021-11-24 | 2021-11-24 | Positioning brain tissue embedding mould |
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| CN217059606U true CN217059606U (en) | 2022-07-26 |
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| CN202122898806.5U Active CN217059606U (en) | 2021-11-24 | 2021-11-24 | Positioning brain tissue embedding mould |
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