CN217052230U - Device for implementing single compression wave dynamic impact loading on cells - Google Patents
Device for implementing single compression wave dynamic impact loading on cells Download PDFInfo
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- CN217052230U CN217052230U CN202121008372.0U CN202121008372U CN217052230U CN 217052230 U CN217052230 U CN 217052230U CN 202121008372 U CN202121008372 U CN 202121008372U CN 217052230 U CN217052230 U CN 217052230U
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Abstract
The application discloses a device for implementing single compressional wave dynamic shock loading to cells includes: a calibration system and an experiment system; a calibration system for determining a calibration function between the stress wave signal and the pressure of the fluid representative of the fluid in the second cell loading device; the experimental system comprises: the gas gun device, the first cell loading device and the single loading extension tube are coaxially arranged; the first cell loading device is loaded with cell suspension; the air cannon device impacts the first cell loading device to generate compression waves, and the compression waves are transmitted in the single-loading extension tube after passing through the first cell loading device and are not reflected any more; wherein the experimental system measures a stress wave signal of the liquid in the first cell loading device and determines the pressure of the liquid in the first cell loading device according to the calibration function. The application can realize effective and stable single compression wave loading on the isolated cells.
Description
Technical Field
The utility model relates to a cell impact damage technique among the biomechanics especially relates to a device of single compression wave dynamic impact loading is implemented to cell.
Background
In the accident such as collision, traffic accident, mine and the like in sports, impact injury is the most extensive type of injury among injured people, seriously damages the health of people, and can cause long-term chronic inflammation of organisms due to the superposition of a series of complex physical injury effects (inertia, stripping and implosion), and possibly induce a plurality of sequelae such as cerebral concussion, Alzheimer and the like. Impact injury is therefore classified as a distinct type of injury independent of penetrating, blunt injury in clinical and basic studies. Currently, there is no strong targeted and effective measure for such impact injury in clinic, so researches on the mechanism of the injury type are urgently needed.
In the research of the damage mechanism, a multi-scale model is a necessary condition for researching the impact damage mechanism. The in vivo model can reduce the systemic chain type injury reaction and can carry out scientific and effective verification on the mechanism of the in vitro model; the in vitro model can be used for mechanism research under few interference factors, and can stably, accurately and effectively simulate an impact physical environment, and is a cornerstone of the whole in vitro model. Therefore, two models are absent in the research of the injury mechanism.
At present, no effective solution is provided in the prior art for the problem of impact loading of ex vivo cells.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a device of single compression wave dynamic shock loading is implemented to cell to solve the unable loaded problem of single shock of carrying out of separation cell that prior art exists.
According to the utility model discloses the embodiment provides a carry out single compressional wave dynamic impact loading device to cell, it includes: calibrating a system and an experimental system; the calibration system is used for measuring a calibration function between a stress wave signal representing the liquid in the second cell loading device and the liquid pressure; the experimental system comprises: the gas gun device, the first cell loading device and the single loading extension tube are coaxially arranged; the proximal end of the first cell loading device is opposite to the air cannon device, and the distal end of the first cell loading device is connected with the single-loading extension tube; wherein the first cell loading device is loaded with a cell suspension; the air cannon device impacts the first cell loading device to generate a compressional wave that propagates through the first cell loading device in the single-load extension tube without being reflected; wherein the experimental system measures a stress wave signal of the liquid in the first cell loading device and determines the pressure of the liquid in the first cell loading device from the calibration function.
Wherein, the experimental system still includes: the strain gauge is used for measuring stress wave signals of liquid in the first cell loading device and sending the measured stress wave signals to the data collector.
Wherein, calibration system includes: the air gun device, the second cell loading device and the hydraulic sensor are coaxially arranged; the proximal end of the second cell loading device is opposite to the air cannon device, and the distal end of the second cell loading device is connected with the hydraulic sensor, wherein the second cell loading device is only loaded with liquid; the air gun device impacts the second cell loading device to generate compression waves, and the hydraulic sensor detects pressure data of liquid in the second cell loading device.
Wherein, calibration system still includes: the strain gauge is used for measuring stress wave signals of liquid in the second cell loading device and sending the measured stress wave signal data to the data collector; and the data acquisition unit calculates the calibration function according to stress wave signal data from the strain gauge and pressure data from the hydraulic sensor.
Wherein the proximal end of the first/second cell loading device is sealed by a sealing ring and the distal end of the first/second cell loading device is sealed by a nitrile rubber membrane.
Wherein, the gas big gun device includes: a bullet and an air cannon, the bullet being placed within the air cannon, the distance between the air cannon and the first/second cell loading device being less than one third of the length of the bullet.
According to the technical scheme of the utility model, realize through single loading extension pipe that the loading of single compression wave has avoided the back wave interference to hoop strain signal through cell loading attachment corresponds with the fluid pressure in the cell loading attachment, has improved the accuracy and the repeatability of experiment.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without undue limitation to the invention. In the drawings:
fig. 1 is a block diagram of a calibration system according to an embodiment of the present invention;
fig. 2 is a schematic diagram of calibration curves for hydraulic pressure and hoop maximum strain in a calibration system according to an embodiment of the present invention;
fig. 3 is a block diagram of an experimental system according to an embodiment of the present invention;
fig. 4 is a schematic diagram of a hoop strain curve propagating in a tube wall resulting from a bullet impact in a cell experiment system according to an embodiment of the present invention;
fig. 5 is a flow chart of a method of single compressional wave dynamic shock loading of cells according to an embodiment of the invention.
Detailed Description
To make the purpose, technical solution and advantages of the present invention clearer, the following will combine the embodiments of the present invention and the corresponding drawings to clearly and completely describe the technical solution of the present invention. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.
The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.
According to the utility model discloses the embodiment provides a device that carries out single compression wave dynamic impact loading to cell, the device includes: calibration systems (or called data calibration systems) and cell experiment systems. Since it is required that the cells are not contaminated when the cells are loaded for a single time, that is, the hydraulic sensor cannot be added to the cell loading device to collect the pressure data of the liquid, it is necessary to perform data calibration, that is, determine a calibration function between the stress wave signal representing the liquid in the cell loading device and the liquid pressure, before the single loading is performed.
Referring to fig. 1, the calibration system includes: a gas gun device 1, a cell loading device 2 (i.e., a second cell loading device), and a hydraulic pressure sensor 3, which are coaxially disposed. Specifically, the gas gun device 1 comprises a bullet 11 and a gas gun 12, and the bullet 11 is placed inside the gas gun 12. The air cannon apparatus 1 is opposite to the proximal end of the cell loading apparatus 2, so that the distance between the air cannon apparatus 1 and the cell loading apparatus 2 is less than one third of the length of the bullet 11, and thus the bullet 11 does not deviate at the outlet of the air cannon 12 and can accurately impact the center of the sealing ring 23 of the cell loading apparatus 2.
The cell loading device 2 is shaped like a syringe and has a sealed cylindrical main body 21, the main body 21 of the cell loading device 2 is filled with liquid only, and the cell suspension is not loaded in the cell loading device of the calibration system. The proximal end of the body 21 has a larger contact circular surface 22 to receive the impact of the bullet 11, the proximal end of the body 21 is sealed by a sealing ring 23, and the distal end of the body 21 can be sealed using a nitrile rubber membrane. The far end of the cell loading device 2 is connected with a hydraulic sensor 3, and the hydraulic sensor 3 is used for detecting pressure data of liquid in the cell loading device 2 and sending the detected pressure data to a data acquisition unit 5.
The cell loading device 2 is provided with a circumferential strain gauge 4, and the circumferential strain gauge 4 is used for measuring stress wave signals of liquid in the cell loading device 3 and sending measured data to the data acquisition unit 5. And the data acquisition unit 5 calculates to obtain a calibration function according to the pressure data detected by the hydraulic sensor 3 and the stress wave data measured by the strain gauge 4.
Referring to fig. 2, fig. 2 is a schematic diagram of calibration curves for hydraulic pressure and hoop maximum strain in a calibration system. The diagram shows the liquid pressure in kPa on the abscissa (X-axis) and the maximum hoop strain in μ ∈onthe ordinate (Y-axis). After a plurality of experiments, the relation of the calibration function is measured to be y-3E-5 x. By the formula, the data of the pressure in the liquid can be obtained by deducing the annular strain.
Referring to fig. 3, an experimental system according to an embodiment of the present application includes: a coaxially arranged gas gun device 1, a cell loading device 6 (i.e. a first cell loading device) and a single-loading extension tube 7. In the present application, the experimental system is the same as most of the devices in the calibration system, and the differences lie in: the cell loading device 6 of the experimental system is loaded with the cell suspension, and the distal end of the cell loading device 6 of the experimental system is sleeved with the single-loading extension tube 7.
Specifically, the distal end of the cell loading device 6 is sleeved in a single-load extension tube 7 loaded with liquid inside, sealing the connection of the cell loading device 6 and the single-load extension tube 7. After the air cannon device 1 impacts the cell loading device 6 to generate the compression wave, the compression wave passes through the cell loading device 6 and then is transmitted in the single-loading extension tube 7 and is not reflected any more, so that the dynamic impact loading of the single compression wave on the cells is realized. The reason why the compression wave is no longer reflected after a single loading of the extension tube 7 is that: the propagation of the compression wave is a column of liquid (in the cell loading device 6) throughout the liquid-nitrile rubber membrane (in the single-loading extension tube 7) during which there is little change in wave impedance and the stress wave continues to propagate forward without being reflected back into the cell loading device 6.
The strain gauge 4 on the cell loading device 6 measures stress wave signals of liquid in the cell loading device 6, measured data are sent to the data acquisition unit 5, and the data acquisition unit 5 can determine the pressure of the liquid in the cell loading device 6 according to the stress wave signals measured by the strain gauge 4 and a calibration function obtained by a calibration system.
Referring to fig. 4, fig. 4 is a schematic diagram of a circumferential strain curve propagating in a pipe wall resulting from a bullet impact in a cellular experimental system. The abscissa of the diagram represents time in ms, and the ordinate represents hoop strain in mV; the rising edge of the oscillogram is about 0.01ms, the subsequent exponential decline meets the function equation of the underwater explosion compression wave, and the loading is proved to be single compression wave loading by the subsequent no reflection wave.
Fig. 5 is a flow diagram of a method of single compressional wave dynamic shock loading of cells according to an embodiment of the present application, comprising:
step S502, coaxially arranging an air gun device, a cell loading device and a hydraulic sensor, and enabling the near end of the cell loading device to be opposite to the air gun device and the far end of the cell loading device to be connected with the hydraulic sensor; wherein the cell loading device is filled with liquid only and is not loaded with a cell suspension;
the cell loading device comprises a cell loading device, a cell loading device and a gas gun device, wherein the gas gun device comprises a gas gun and a bullet arranged in the gas gun, and the distance between the gas gun and the cell loading device is smaller than one third of the length of the bullet.
Step S504, arranging a strain gauge on the cell loading device, and enabling the air cannon device to impact the cell loading device to generate compression waves; detecting pressure data of liquid in the cell loading device through the hydraulic sensor, and measuring stress wave signal data of the liquid in the cell loading device through the strain gauge; calculating to obtain a calibration function according to the stress wave signal data and the pressure data;
step S506, loading cell suspension in the cell loading device, and replacing the hydraulic sensor with a single-loading extension tube to connect the hydraulic sensor to the far end of the cell loading device; causing the gas cannon device to impact the cell loading device to generate a compressional wave that propagates through the cell loading device in the single-charge extension tube without being reflected;
step S508, measuring stress wave signal data of the liquid in the cell loading device through the strain gauge, and determining the pressure of the liquid in the cell loading device when single compression wave loading is carried out according to the calibration function.
Since the cell loading device contains a liquid, it is necessary to seal the cell loading device. In practice, the proximal end of the cell loading device may be sealed with a sealing ring and the distal end of the cell loading device may be sealed with a nitrile rubber membrane.
This application realizes through single loading extension tube that the loading of single compression wave has avoided the back wave to disturb to hoop strain signal through cell loading device corresponds with liquid sensor pressure (being the liquid internal pressure), has improved the accuracy and the repeatability of experiment. In addition, the bullet is directly adopted to impact the cell loading device to realize the loading process, so that the whole cell impact device is concise, the complexity of the stress wave propagation process is reduced, theoretical analysis and calibration are easy to perform, and the whole experiment time is effectively reduced. Because of the advantages, the utility model discloses can realize carrying out effective stable single compression wave loading to the isolated cell.
The above description is only an example of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (6)
1. A device for single compressional wave dynamic shock loading of cells, comprising: a calibration system and an experiment system; the calibration system is used for measuring a calibration function between a stress wave signal representing the liquid in the second cell loading device and the liquid pressure;
the experimental system comprises: the gas gun device, the first cell loading device and the single loading extension tube are coaxially arranged; the proximal end of the first cell loading device is opposite to the air cannon device, and the distal end of the first cell loading device is connected with the single-loading extension tube; wherein the first cell loading device is loaded with a cell suspension; the gas cannon apparatus impacts the first cell loading apparatus to generate a compression wave that propagates in the single-loading extension tube after passing through the first cell loading apparatus and is no longer reflected;
wherein the experimental system measures a stress wave signal of the liquid in the first cell loading device and determines the pressure of the liquid in the first cell loading device according to the calibration function.
2. The apparatus of claim 1, wherein the assay system further comprises: the strain gauge is used for measuring stress wave signals of liquid in the first cell loading device and sending the measured stress wave signals to the data collector.
3. The apparatus of claim 1, wherein the calibration system comprises: the air gun device, the second cell loading device and the hydraulic sensor are coaxially arranged; the proximal end of the second cell loading device is opposite to the air cannon device, the distal end of the second cell loading device is connected with the hydraulic sensor, and only liquid is loaded in the second cell loading device; the air gun device impacts the second cell loading device to generate compression waves, and the hydraulic sensor detects pressure data of liquid in the second cell loading device.
4. The apparatus of claim 3, wherein the calibration system further comprises: the strain gauge is used for measuring stress wave signals of liquid in the second cell loading device and sending the measured stress wave signal data to the data collector; and the data acquisition unit calculates the calibration function according to stress wave signal data from the strain gauge and pressure data from the hydraulic sensor.
5. The device of claim 1 or 3, wherein the proximal end of the first/second cell loading device is sealed by a sealing ring and the distal end of the first/second cell loading device is sealed by a nitrile rubber-based membrane.
6. The apparatus of claim 1 or 3, wherein the gas gun apparatus comprises: a bullet and an air cannon, said bullet being positioned within said air cannon, the distance between said air cannon and said first/second cell loader being less than one third of the length of said bullet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121008372.0U CN217052230U (en) | 2021-05-12 | 2021-05-12 | Device for implementing single compression wave dynamic impact loading on cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121008372.0U CN217052230U (en) | 2021-05-12 | 2021-05-12 | Device for implementing single compression wave dynamic impact loading on cells |
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| CN217052230U true CN217052230U (en) | 2022-07-26 |
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| CN202121008372.0U Active CN217052230U (en) | 2021-05-12 | 2021-05-12 | Device for implementing single compression wave dynamic impact loading on cells |
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- 2021-05-12 CN CN202121008372.0U patent/CN217052230U/en active Active
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