CN217033967U - Device for detecting biomarkers - Google Patents
Device for detecting biomarkers Download PDFInfo
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- CN217033967U CN217033967U CN202220712828.XU CN202220712828U CN217033967U CN 217033967 U CN217033967 U CN 217033967U CN 202220712828 U CN202220712828 U CN 202220712828U CN 217033967 U CN217033967 U CN 217033967U
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- 238000005259 measurement Methods 0.000 claims description 7
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- 238000001514 detection method Methods 0.000 abstract description 14
- 239000004005 microsphere Substances 0.000 description 50
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- 238000003018 immunoassay Methods 0.000 description 2
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- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
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- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
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Abstract
The present invention provides a device for detecting biomarkers comprising: containment structure (100), it places when vertical state, includes from bottom to top: a reaction-accommodating part (101); a measuring part (102) which is positioned above the reaction accommodating part (101) and which penetrates the interior of the reaction accommodating part (101); wherein, a graduated scale (1021) is arranged on the side surface of the measuring part (102); a connecting part (103) that connects the reaction accommodating part (101) and the measuring part (102); the side wall of the connecting part (103) is respectively and obliquely arranged at the joint of the reaction accommodating part (101) and the measuring part (102) at a designated angle; and a magnet (200) provided at the bottom of the reaction accommodating section (101). Thus, it is advantageous to realize efficient detection without requiring a professional device, while simplifying the operation of detecting the biomarker.
Description
Technical Field
The present invention relates to the fields of life sciences and medicine, and in particular, to an apparatus for detecting biomarkers.
Background
Biomarkers (Biomarker) refer to biochemical markers that can mark changes or changes that may occur in the structure or function of systems, organs, tissues, cells, and subcellular structures, such as nucleic acids, proteins, enzymes, carbohydrates, and the like. Biomarkers are commonly used in disease diagnosis, to determine the stage, development and severity of disease, or to evaluate the safety and effectiveness of new drugs or therapies in a target population. Therefore, the detection of biomarkers is of great clinical significance.
Currently, the detection of biomarkers is mainly based on the principle of antigen-antibody specific binding. Clinically, sandwich immunoassays are the most widely used biomarker detection methods. Conventional sandwich immunoassays such as enzyme-linked immunosorbent assay, etc. require some types of labels (e.g., enzymes, fluorescent labels), etc., and also require special instruments (e.g., enzyme-linked immunosorbent assay) and many cumbersome washing steps.
In recent years, methods for detecting markers using microspheres have become a focus of research. The antibody is modified on the surface of the microsphere, and the microsphere is connected by utilizing the specific binding capacity of the antibody antigen to form a polymer. The number of polymers (or the number of individual microspheres remaining) directly corresponds to the target marker content. However, in order to obtain the number of the polymer (or the number of the remaining single microspheres), the currently used schemes such as a coulter counter or image processing after optical photographing require specialized equipment, and the detection process is complicated, which is not favorable for practical operation.
Therefore, there is a need for a device for detecting biomarkers to overcome the problems of the prior art, such as requiring professional equipment for detection, complicated detection process, and inconvenience for practical operation.
SUMMERY OF THE UTILITY MODEL
In view of this, the present application provides a device for detecting a biomarker, and the device is used for detecting the biomarker, so as to overcome the defects that professional equipment is required during detection, the detection process is complicated, and the actual operation is not facilitated in the prior art. The method realizes high-efficiency detection with simple operation and without professional equipment.
The present application provides an apparatus for detecting biomarkers comprising:
containment structure (100), when placed in a vertical state, comprising from bottom to top:
a reaction-accommodating part (101);
a measuring part (102) which is positioned above the reaction accommodating part (101) and which penetrates the interior of the reaction accommodating part (101); wherein a graduated scale (1021) is arranged on the side surface of the measuring part (102); the measuring part (102) is a measuring part (102) made of transparent material;
a connecting portion (103) that connects the reaction accommodating portion (101) and the measurement portion (102); the side wall of the connecting part (103) is obliquely arranged at the joint of the reaction accommodating part (101) and the measuring part (102) at a specified angle;
wherein the apparatus for detecting a biomarker further comprises:
a magnet (200) disposed at the bottom of the reaction accommodating portion (101).
The method comprises the steps that a solution containing a target biomarker (301) and other biomarkers (302) is placed in a containing structure (100), two types of microspheres are added into the solution and are fully mixed with the solution, one of the two types of microspheres is microspheres (401) with density smaller than that of water, such as hollow glass spheres or microspheres made of other materials with density smaller than that of water, the diameter of the microspheres is 1-500 micrometers, preferably 1-100 micrometers, and the surface of the microspheres is modified with a first antibody (4011) corresponding to the target biomarker; the other is magnetic microspheres (402) with a diameter of 1-500 microns, preferably 1-100 microns, surface modified with a second antibody (4021) corresponding to the target biomarker. After being fully mixed, the two microspheres can grasp the target biomarker in the solution and form dimers with each other (most of the microspheres form dimers, and a small part of the microspheres form polymers which can be ignored). At this point, a magnet (200) is placed at the bottom of the vessel and all the magnetic microspheres are attracted to the bottom of the solution. Therefore, those magnetic microspheres that form dimers will aggregate to the bottom of the solution with the hollow microspheres. Then, a certain amount of the solution was added to the vessel to raise the liquid level to a prescribed position. The hollow microspheres which do not form the dimer rise along with the rise of the liquid level, and because the buoyancy of the hollow microspheres is larger than the gravity, the hollow microspheres are closely arranged in the measuring part (102) from top to bottom. A scale (1021) is arranged on the side surface of the measuring part (102). According to the length of the hollow microspheres which are not formed with the dimer and are measured by the graduated scale in the measuring part, the number of the hollow microspheres which are not formed with the dimer (monomer) can be basically quantified according to the circumference of the measuring part and the diameter of the hollow glass spheres, and the number of the hollow microspheres which are not formed with the dimer (monomer) can be deducted according to the total number of the added hollow microspheres to deduct the number of the hollow microspheres which are not formed with the dimer (monomer), so that the content of the target biomarker in the solution can be deduced. Thus, the detection is simple to operate and efficient without professional equipment.
The side wall of the connecting part (103) is inclined at a specified angle with the joint of the reaction accommodating part (101) and the measuring part (102), so that the hollow glass microspheres can float up to the measuring part (102) from the reaction accommodating part (101) more easily.
Preferably, the circumference of the measurement portion (102) is smaller than the circumference of the reaction site (101).
From above, the circumference of the measuring part (102) may be smaller than the circumference of the reaction accommodating part (101) according to actual needs. The scale of the hollow glass ball on the side surface of the measuring part (102) is measured.
Preferably, the upper end of the measuring part (102) is opened or covered.
Therefore, the upper end opening of the measuring part (102) can be arranged or covered according to actual needs.
Preferably, the capping arrangement comprises: a removable cover or flip cover arrangement is provided.
Preferably, when the flip cover is arranged, the flip cover is pivoted with the opening at the upper end of the measuring part (102) through a flip cover pivoting shaft.
Preferably, the scales (1021) arranged on the side surface of the measuring part (102) are uniformly arranged scales.
In this way, the scales (1021) provided on the side surface of the measuring section (102) can be uniformly provided scales. The device is beneficial to more accurate measurement, and can be set to be specified scales according to actual needs.
Preferably, the magnet (200) is removably disposed at the bottom of the reaction accommodating portion (101).
Preferably, the measuring portion (102) is a tubular structure.
From above, the measuring portion (102) may be a tubular structure with a uniform circumference from bottom to top.
To sum up, the application provides a device for detecting biomarker, detects biomarker through the device, has overcome the equipment that needs the specialty when detecting in prior art, and the testing process is comparatively complicated, is unfavorable for actual operation's defect. The method realizes high-efficiency detection with simple operation and without professional equipment.
Drawings
Fig. 1 is a front view of an apparatus for detecting biomarkers provided by an embodiment of the present application;
FIG. 2 is a schematic representation of the binding of two microspheres to a target biomarker provided in an example of the present application;
fig. 3 is a schematic diagram of two microspheres provided in an example of the present application that bind to a target biomarker to form a dimer in a device for detecting biomarkers.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived from the embodiments of the present invention by a person skilled in the art, are within the scope of the present invention.
The present application provides a device for detecting a biomarker, which facilitates efficient detection that is easy to operate and does not require specialized equipment when detecting a biomarker.
The present application provides a device for detecting biomarkers as shown in figure 1, comprising:
containment structure (100), when placed in a vertical state, comprising from bottom to top:
a reaction-accommodating part (101);
a measuring part (102) which is positioned above the reaction accommodating part (101) and which penetrates the interior of the reaction accommodating part (101); wherein a graduated scale (1021) is arranged on the side surface of the measuring part (102); the measuring part (102) is made of transparent material (102);
a connecting portion (103) that connects the reaction accommodating portion (101) and the measurement portion (102); the side wall of the connecting part (103) is obliquely arranged at the joint of the reaction accommodating part (101) and the measuring part (102) at a specified angle;
the reaction-containing part (101) and the connecting part (103) may be made of a transparent material, or may be made of other materials.
Wherein the apparatus for detecting a biomarker further comprises:
a magnet (200) provided at the bottom of the reaction accommodating section (101).
Wherein the circumference of the measurement portion (102) is smaller than the circumference of the reaction site (101). From above, the circumference of the measuring part (102) may be smaller than the circumference of the reaction site (101) according to actual needs. The scale of the hollow glass ball on the side surface of the measuring part (102) is measured.
Wherein the upper end opening of the measuring part (102) is arranged or covered. Therefore, the upper end opening of the measuring part (102) can be arranged or covered according to actual needs.
Wherein, add the lid setting, include: a removable cover or flip cover arrangement is provided.
When the cover is arranged, the cover is pivoted with the opening at the upper end of the measuring part (102) through the cover pivoting shaft.
The graduated scales (1021) arranged on the side face of the measuring part (102) are uniformly arranged graduated scales. In this way, the scales (1021) provided on the side surface of the measuring section (102) can be uniformly provided scales. The method is favorable for more accurate measurement, and can be set to be specified scales according to actual needs.
Wherein the magnet (200) is removably arranged at the bottom of the reaction accommodating part (101).
Wherein the measuring part (102) is of a tubular structure. From above, the measuring part (102) may be a tubular structure with a uniform circumference from bottom to top.
For a more clear explanation of the technical solution of the present application, the working principle of the device for detecting biomarkers of the present application will now be explained as follows:
referring first to FIG. 2, two types of microspheres useful in the present invention will be described, one being less dense than water, such as hollow glass spheres or microspheres made from other polymeric materials having a density less than water. The other is magnetic microspheres, such as ferroferric oxide magnetic microspheres. The surfaces of the two microspheres are respectively modified with primary antibody (4011) and secondary antibody (4021) aiming at the target marker. When the target marker (301) is present in the solution, microsphere aggregates as shown in FIG. 2 may be formed by mixing. The diagram shows a dimer, most of which is dimer in probability, and a small part of which forms trimer or even tetramer is negligible.
Further, the working principle of the device for detecting biomarkers is explained as follows:
as shown in fig. 3, a solution containing a target biomarker (301) and other biomarkers (302) is placed in the containing structure (100), and two types of microspheres are added into the solution and mixed sufficiently, wherein one of the two types of microspheres is a hollow glass sphere with a density smaller than that of water, and the other is a magnetic microsphere, and after sufficient mixing, the two types of microspheres can grasp the target biomarker in the solution and form dimers with each other (most of the microspheres form dimers, and a small part of the microspheres form negligible multimers). At this point, a magnet (200) is placed at the bottom of the vessel and all the magnetic microspheres are attracted to the bottom of the solution. Therefore, those magnetic microspheres that form dimers will aggregate to the bottom of the solution with the hollow microspheres. Then, a certain amount of the solution was added to the vessel to raise the liquid level to a prescribed position. As shown in fig. 1, hollow microspheres, which are not formed into dimers and have a density lower than that of water, rise as the liquid level rises. A scale (1021) is arranged on the side surface of the measuring part (102). The length of the hollow microspheres which do not form the dimer and gather in the measuring part is measured according to the graduated scale, the number of the hollow microspheres which do not form the dimer (monomer) can be basically quantified according to the circumference of the measuring part and the diameter of the hollow glass spheres, the number of the hollow microspheres which are not formed the dimer (monomer) can be deducted according to the total number of the added hollow microspheres, the number of the hollow microspheres which are combined with the target biomarker can be deduced, and then the content of the target biomarker in the solution can be deduced.
To sum up, the application provides a device for detecting biomarker, detects biomarker through the device, has overcome the equipment that needs the specialty when detecting in prior art, and the testing process is comparatively complicated, is unfavorable for actual operation's defect. The method realizes high-efficiency detection with simple operation and without professional equipment.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. An apparatus for detecting a biomarker, comprising:
containment structure (100), it places when vertical state, includes from bottom to top:
a reaction-accommodating part (101);
a measuring part (102) which is positioned above the reaction accommodating part (101) and which penetrates the interior of the reaction accommodating part (101); wherein a graduated scale (1021) is arranged on the side surface of the measuring part (102); the measuring part (102) is a measuring part (102) made of transparent material;
a connecting portion (103) that connects the reaction accommodating portion (101) and the measurement portion (102); the side wall of the connecting part (103) and the joint of the reaction accommodating part (101) and the measuring part (102) are obliquely arranged at a designated angle;
wherein the apparatus for detecting a biomarker further comprises:
a magnet (200) provided at the bottom of the reaction accommodating section (101).
2. The device for detecting biomarkers according to claim 1, wherein the circumference of said measuring portion (102) is smaller than the circumference of said reaction site (101).
3. The device for detecting biomarkers according to claim 1, wherein the upper end of the measuring part (102) is open or capped.
4. The device for detecting biomarkers according to claim 3, wherein said capping arrangement comprises: a removable cover or flip cover arrangement is provided.
5. The apparatus for detecting biomarkers according to claim 4, wherein when provided for the flip, the flip is pivoted with the upper end opening of the measuring part (102) through a flip pivoting shaft.
6. The apparatus for detecting biomarkers according to claim 2, wherein the scales (1021) provided on the side of the measuring part (102) are uniformly provided scales.
7. The device for detecting biomarkers according to claim 1, wherein the magnet (200) is removably arranged at the bottom of the reaction containment (101).
8. The device for detecting biomarkers according to claim 1, wherein the measuring part (102) is a tubular structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220712828.XU CN217033967U (en) | 2022-03-29 | 2022-03-29 | Device for detecting biomarkers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220712828.XU CN217033967U (en) | 2022-03-29 | 2022-03-29 | Device for detecting biomarkers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217033967U true CN217033967U (en) | 2022-07-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220712828.XU Expired - Fee Related CN217033967U (en) | 2022-03-29 | 2022-03-29 | Device for detecting biomarkers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217033967U (en) |
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2022
- 2022-03-29 CN CN202220712828.XU patent/CN217033967U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220722 |
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| CF01 | Termination of patent right due to non-payment of annual fee |