CN217007351U - Rapid quantitative xerophthalmia diagnosis immunoassay reagent card - Google Patents

Abstract

The application discloses quick quantitative xerophthalmia diagnosis immunoassay reagent card relates to external diagnosis technical field, including sampling card, last card shell, test paper strip and lower card shell, the sampling card is including detection window and tear sampling piece, and the test paper strip includes water absorption pad, cellulose nitrate membrane, combination pad and the sample pad that pastes in proper order above and the bottom plate, matrix metalloproteinase 9(MMP-9) antibody and a lymphotoxin (LTa) antibody that have laid the marker mark on the combination pad, be equipped with quality control line, detection line on the cellulose nitrate membrane, this quick quantitative xerophthalmia diagnosis immunoassay reagent card has the detection convenience, and the detection speed is fast, detect advantages such as the specificity is strong, sensitivity height, can carry out ration, semi-quantitative analysis.

Description

Rapid quantitative xerophthalmia diagnosis immunoassay reagent card

Technical Field

The utility model relates to the technical field of in-vitro diagnosis, in particular to a rapid quantitative dry eye diagnosis immunoassay reagent card.

Background

Early rapid diagnosis and timely treatment are therefore critical in determining prognosis. The previous tear osmolarity has been considered the gold standard, with over 312mOsol/L as a dry eye diagnostic standard, but it has not been widely used for two reasons. One is that tear osmolality is also elevated in normal humans and poorly correlated with the signs of clinical dry eye, and changes in osmolality follow the symptoms and goblet cell changes, which cannot be an early diagnosis. Secondly, the osmotic pressure detection system has poor repeatability (the continuous detection error of the same individual reaches 35 mOsm/l), large variation degree of results (the sensitivity is 64-73 percent, and the specificity is 71-92 percent), and high cost, so that the osmotic pressure detection system cannot be widely applied.

The fluorescence immunochromatography technology is a novel membrane detection technology based on antigen-antibody specific immunoreaction. The technology takes strip fiber chromatography materials fixed with a detection line and a quality control line as a stationary phase, a test solution as a mobile phase, a fluorescence labeling antibody or antigen is fixed on a binding pad, and an analyte moves on the chromatography strip through capillary action.

The detection sensitivity and accuracy can be improved by selecting one or more specific molecular markers and combining the specific molecular markers with a fluorescence immunochromatographic technology.

The detection method of the specificity marker MMP-9 tear in the tear is applied in the United states for many years, the sensitivity reaches 86 percent, the specificity reaches 97 percent, and the expression of MMP-9 is also related to the severity of the disease. Thus detection of elevated MMP-9 levels may allow early diagnosis and assessment of the condition. But also has the following disadvantages: the method cannot quantify, cannot monitor the change of the illness state after treatment, and has large variation degree. The large variability is a big difficulty for dry eye diagnosis, including Schirmer and BUT examination, and the data difference is large when the instrument is operated under different conditions.

Evidence shows that Lymphotoxin a (LTa) is involved in the maintenance of ocular surface immune balance of dry eye through an LTa-TNFR2-Treg axis, and a significant reduction in the level of Lymphotoxin a (LTa) in tears of dry eye patients is found through a lacrimal proteomics study. This result suggests that lymphotoxin a (LTa) may be useful as a molecular marker of tears for assessing ocular surface immune homeostasis, aiding in the diagnosis of dry eye and guiding treatment.

SUMMERY OF THE UTILITY MODEL

In order to improve the above-mentioned problems, the present invention provides a rapid quantitative dry eye diagnostic immunoassay reagent card.

The utility model provides a rapid quantitative xerophthalmia diagnosis immunoassay reagent card, which adopts the following technical scheme:

a quick quantitative xerophthalmia diagnosis immunodetection reagent card which is characterized in that: including sampling card, go up card shell, test paper strip and lower card shell, there are absorbent pad, nitrocellulose membrane, combination pad, sample pad and bottom plate on the test paper strip, be equipped with a matter control line and two detection lines on the nitrocellulose membrane, and two detection lines are detection line a and detection line b respectively.

Optionally, the sampling card is provided with a detection window and a tear sampling sheet.

Optionally, the upper clamping shell is provided with a detection window a, a sample adding hole and a liquid adding hole.

Optionally, the test strip has only one quality control line.

Optionally, the substrate of the test strip is a low-fluorescence background PVC substrate.

Optionally, the combination pad 11 and the sample pad on the test strip are made of glass fiber.

Optionally, the absorbent pad is made of filter paper.

In summary, the utility model includes at least one of the following advantages:

1. the immunochromatography is combined with the tear specific marker, so that the xerophthalmia can be diagnosed more quickly and accurately.

2. The two specific molecular markers make the diagnosis result of xerophthalmia more reliable and more suitable for different conditions of patients.

3. The sampling card and the detection test paper strip integrated structure are more exquisite, and the detection is more convenient.

Drawings

FIG. 1 is a block diagram of a sample card of the present invention;

FIG. 2 is a top plan view of the upper card housing of the present invention;

FIG. 3 is a diagram of the structure of the test strip of the present invention;

FIG. 4 is a bottom view of the lower card housing of the present invention.

Description of reference numerals:

1. sampling cards; 2. detecting a window; 3. a tear sampling sheet; 4. mounting a clamping shell; 5. detecting a window a; 6. A sample application hole; 7. a liquid feeding hole; 8. a test strip; 9. a water absorbent pad; 10. a nitrocellulose membrane; 11. a bonding pad; 12. a sample pad; 13. a base plate; 14. a quality control line; 15. detecting a line a; 16. detecting a line b; 17. And (5) clamping the shell.

Detailed Description

The utility model is described in further detail below with reference to figures 1-4.

Referring to fig. 1, a first embodiment is provided: a rapid quantitative dry eye diagnosis immunity detection reagent card sampling card 1, the sampling card 1 is provided with a detection window 2 and a tear sampling sheet 3, the tear sampling sheet 3 is covered on the position of a sample adding hole 6 of an upper card shell 4 after sampling on an eyelid, so that the detection window 2 is just exposed out of a detection window a5 of the upper card shell 4.

Referring to fig. 2, a second embodiment is provided: a detection window a5, a sampling hole 6 and a liquid adding hole 7 are arranged on an upper card shell 4 of a rapid quantitative xerophthalmia diagnosis immunodetection reagent card, a test strip 8 is embedded into the upper card shell 17, the detection window a5 just exposes a quality control line 14, a detection line a15 and a detection line b16, the sampling hole 6 is a position for placing a tear sampling sheet 3 in a sampling card 1, and the liquid adding hole 7 is a position for dripping a buffer solution.

Referring to fig. 3, a third embodiment is provided: the utility model provides a quick quantitative xerophthalmia diagnosis immunodetection test paper strip 8, is pasted on bottom plate 13 in proper order by absorbent pad 9, nitrocellulose membrane 10, combination pad 11, sample pad 12, and nitrocellulose membrane 10 upper bag is equipped with quality control line 14, detection line a15 and detection line b16, and absorbent pad 9 is the filter paper material, and combination pad 11 and sample pad 12 are the glass fiber membrane, and bottom plate 13 is the PVC offset plate of low fluorescence background.

The quality control line 14 is a second antibody of MMP-9 antibody I or LTa antibody I, or a mixture of MMP-9 antibody I and LTa antibody I, the detection line a15 is MMP-9 antibody II with different binding sites with the labeled MMP-9 antibody I, the detection line b16 is LTa antibody II with different binding sites with the labeled LTa antibody I, or the detection line a15 is LTa antibody II with different binding sites with the labeled LTa antibody I, and the detection line b16 is MMP-9 antibody II with different binding sites with the labeled MMP-9 antibody I.

The combination pad 11 on the test strip 8 is uniformly paved with a mixture of MMP-9 antibody I and LTa antibody I marked by markers, and the markers combined with the antibodies can be one or more of quantum dots, magnetic particles, nano metal ions, latex particles, carbon nanoparticles, rare earth ions and fluorescent particles.

The position of the gap between the bonding pad 11 and the sample pad 12 on the test strip 8 is the position where the tear sampling sheet 3 is placed, so that the tear sampling sheet is exposed from the position of the sample application hole 6 after being embedded in the upper and lower card cases 17.

Referring to fig. 4, a fourth embodiment is provided: a rapid quantitative dry eye diagnostic immunoassay reagent card lower card shell 17 embeds a test strip 8 therein together with an upper card shell 4.

The above are all preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, so: equivalent changes made according to the structure, shape and principle of the utility model shall be covered by the protection scope of the utility model.

Claims (7)

1. A quick quantitative dry eye diagnosis immunodetection reagent card which is characterized in that: including sampling card (1), go up kaka shui (4), test paper strip (8) and kaka shui (17) down, there are absorbent pad (9), nitrocellulose membrane (10), combination pad (11), sample pad (12) and bottom plate (13) on test paper strip (8), be equipped with a matter accuse line (14) and two detection lines on nitrocellulose membrane (10), and two detection lines are detection line a (15) and detection line b (16) respectively.

2. The immunoassay reagent card for rapid quantitative dry eye diagnosis according to claim 1, wherein: the sampling card (1) is provided with a detection window (2) and a tear sampling sheet (3).

3. The rapid quantitative dry eye diagnostic immunoassay reagent card of claim 1, wherein: and the upper clamping shell (4) is provided with a detection window a (5), a sample adding hole (6) and a liquid adding hole (7).

4. The immunoassay reagent card for rapid quantitative dry eye diagnosis according to claim 1, wherein: the test strip (8) is provided with only one quality control line (14).

5. The immunoassay reagent card for rapid quantitative dry eye diagnosis according to claim 1, wherein: the bottom plate (13) of the test strip (8) is a low-fluorescence background PVC bottom plate.

6. The immunoassay reagent card for rapid quantitative dry eye diagnosis according to claim 1, wherein: the combination pad (11) and the sample pad (12) on the test strip (8) are made of glass fiber materials.

7. The immunoassay reagent card for rapid quantitative dry eye diagnosis according to claim 1, wherein: the water absorption pad (9) is made of filter paper.

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