CN217033301U - Special leak protection embedding bag of small tissue - Google Patents

Abstract

The utility model belongs to the field of pathological experiment tools, and particularly discloses a special leak-proof embedding bag for tiny tissues, which comprises a bag body and a sealing structure arranged on the bag body; wherein, one side of the bag body is provided with an opening to form a taking and placing opening for taking and placing the micro tissues; the sealing structure is arranged at the corresponding position of the taking and placing opening. The utility model has ingenious structural design, effectively solves the problem that the tissue is easy to lose in the dehydration and embedding process due to small volume, omits the operation of fixation, and has the advantages of easy operation, easy control, good sealing property, low price and the like; meanwhile, when the micro tissue is stored, the operation of pre-fixing can be omitted, the use is simple, convenient and quick, and the micro tissue embedding box can be used in a matched manner, so that the flexibility is good.

Description

Special leak protection embedding bag of small tissue

Technical Field

The utility model relates to a pathological experiment tool, in particular to a special leakage-proof embedding bag for a micro tissue.

Background

Paraffin section is the most widely used method in conventional histological slide preparation technology, and is not only used for observing morphological structure of normal cell tissue, but also used as the main method for researching, observing and judging morphological change of cell tissue in pathology, legal medicine and other subjects, and is widely used in research in other subject fields.

In clinical and experimental procedures, there are often tiny, scattered samples that require morphological analysis of internal cell tissues, such as 3D tumor cell balls that mimic solid tumors, small intestine organoids used to study intestinal disease, laboratory mouse glands, and hospital patient endoscopes and needle biopsies, which may be as short as 0.5-5mm in length. Because the micro tissues are small in size, broken in quality and easy to lose; the water is easy to leak out from the gaps of the embedding box during dehydration; the paraffin liquid which is easy to melt is dispersed and easy to float during embedding; when in slicing, a plurality of small tissues are not easy to be cut completely, the tissues are easy to be cut to a few, even completely, and the like, which brings certain difficulty to pathological section making.

In the prior art, agar is mostly adopted for pre-fixing the micro tissue during experiments, so that the tissue can be prevented from being lost or mixed with other tissues in the subsequent operation process. However, the agar concentration is required to be adjusted according to the tissue condition when the agar is used for pre-fixation, the embedding concentration of the agar is difficult to control, the use concentration is high (more than 3 percent), the gelation is too rapid, the dehydration is difficult, the coagulation cannot be well performed when the concentration is too low, and the coagulation is serious after the dehydration. Therefore, it is necessary to design a leak-proof device for micro-tissues, which is easy to handle, inexpensive, and easy to control.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects of the prior art and provides a special leak-proof embedding bag for a micro tissue, which is simple and convenient to operate, has a good leak-proof effect and is beneficial to storing the micro tissue.

The purpose of the utility model is realized by the following technical scheme:

a special leakproof embedding bag for micro tissues comprises a bag body and a sealing structure arranged on the bag body; wherein, one side of the bag body is provided with an opening to form a taking and placing opening for taking and placing the micro tissues; the sealing structure is arranged at the corresponding position of the taking and placing opening.

The working principle of the special leak-proof embedding bag for the tiny tissues is as follows:

when the tiny tissue is deposited to needs, can borrow instruments such as medical or experiment pliers, clip, open seal structure to open the getting of the bag body and put into mouthful, put into the tiny tissue afterwards, and close seal structure, thereby accomplish depositing of tiny tissue. At this time, the experimental treatment may be directly performed on the embedding bag containing the minute tissue, or a plurality of embedding bags containing the minute tissue may be put into an embedding cassette so as to perform the batch experiment. When the micro tissue needs to be taken out, the micro tissue can be directly taken out by using tools such as pliers, clips and the like after the sealing structure is opened.

In a preferred embodiment of the present invention, the sealing structure is a clip chain self-sealing strip structure, the clip chain self-sealing strip structure includes a rib and a groove, and the rib and the groove are respectively disposed on two inner side surfaces of the bag body.

Preferably, the sealing structure is arranged on the inner side of the opening edge of the taking and placing opening; the sealing structure divides the bag body into a storage area and a marking area, wherein the storage area is formed between the sealing structure and the bottom of the bag body, and the marking area is formed between the sealing structure and the edge of the taking and placing opening.

Preferably, the mark area comprises a blank area and a hole mark area, and the hole mark area is provided with a plurality of holes to be marked which can be punctured as marks.

Preferably, the number of the holes to be marked is 9.

Preferably, the size of the pouch body is 1cm × 1 cm.

Preferably, the size of the marking zone is 1cm × 0.4 cm.

In a preferred embodiment of the present invention, the bag body is made of a filter membrane material.

Preferably, the pouch body is made of a cellulose acetate filter.

Preferably, the cellulose acetate filter mesh has a mesh size of 100 mesh.

Compared with the prior art, the utility model has the following beneficial effects:

1. the utility model has the advantages of ingenious structural design, capability of effectively solving the problem that the tissue is easy to lose in the dehydration and embedding process due to small volume, no operation of fixation, easiness in operation and control, good sealing property, low price and the like.

2. The embedding bag can save the operation of pre-fixing when storing the micro tissues, is simple, convenient and quick to use, can be matched with an embedding box for use, and has good flexibility.

3. The embedded bag is used for loading the micro tissue, so that the tissue can be conveniently and independently moved, and the tissue is effectively prevented from being damaged in the moving process.

Drawings

Fig. 1 is a schematic plan view of one embodiment of the present invention.

Detailed Description

The present invention will be further described with reference to the following examples and drawings, but the embodiments of the present invention are not limited thereto.

Referring to fig. 1, the leakage-proof embedding bag for micro-tissues of the present embodiment includes a bag body 1 and a sealing structure disposed on the bag body 1; wherein, one side of the bag body 1 is provided with an opening to form a taking and placing opening for taking and placing the micro tissues; the sealing structure is arranged at the corresponding position of the taking and placing opening.

Referring to fig. 1, the sealing structure is a clip chain self-sealing strip structure, the clip chain self-sealing strip structure comprises a convex edge 5 and a groove 6, and the convex edge 5 and the groove 6 are respectively arranged on two inner side surfaces of the bag body 1. The sealing structure of the embodiment is of a zipper self-sealing strip structure, is simple in structure, convenient to manufacture, low in price, convenient to operate, capable of being used repeatedly, good in sealing effect and beneficial to preventing leakage of micro tissues. Further, the sealing structure in this embodiment may also adopt other structures, such as a slider-type clip chain structure.

Referring to fig. 1, the sealing structure is arranged inside the opening edge of the pick-and-place port; the sealing structure divides the bag body 1 into a storage area 2 and a marking area 3, wherein the storage area 2 is formed between the sealing structure and the bottom of the bag body 1, and the marking area 3 is formed between the sealing structure and the edge of the access opening. The marking area 3 comprises a blank area and a hole mark area, and a plurality of holes to be marked 4 which can be punctured as marks are arranged on the hole mark area; specifically, 9 holes 4 to be marked are provided, and the 9 holes 4 to be marked are arranged in a 3 × 3 layout; of course, other arrangements, such as a linear arrangement, may be used.

Referring to fig. 1, in this embodiment, the blank area and the hole mark area are distributed left and right. The bag body 1 is divided into the storage area 2 and the marking area 3 by the sealing structure, on one hand, related information can be written on a blank area of the marking area 3 so as to be distinguished, and meanwhile, the hole 4 to be marked can be quickly punctured by a tool such as a pencil, so that the embedding bag can be identified and classified; on the other hand, with seal structure setting in the inboard of getting and putting a mouthful border, further reduced the space of depositing the little tissue on the basis of the bag body 1, be favorable to improving the stability when the little tissue is deposited, reduce and spill the risk, improve sealed effect.

In this embodiment, the size of the bag body 1 is 1cm × 1 cm. With the bag body 1 of such a size, it is sufficient to store minute tissues having a length of 5mm or less, and tissues having a length of more than 5mm can be directly loaded using a general-purpose embedding cassette. The size of the marking zone 3 of this embodiment is 1cm × 0.4 cm.

In this embodiment, the bag body 1 is made of a filter membrane material. Is made of a cellulose acetate filter screen. In this way, the organic reagents used in the experiments can be allowed to pass freely through the pouch 1 without reacting at the same time. Wherein, the mesh number of cellulose acetate filter screen is 100 meshes, and the sieve mesh size is 0.150mm, can keep minute tissue in bag 1.

Referring to fig. 1, the working principle of the leakproof embedding bag special for the micro tissue of the embodiment is as follows:

when the tiny tissue is deposited to needs, can borrow instruments such as medical or experiment pliers, clip, open seal structure to open the getting of the bag body 1 and put into mouthful, put into the tiny tissue afterwards, and close seal structure, thereby accomplish depositing of tiny tissue. At this time, a pencil can be used to write related information on the blank area of the marking area 3, or to puncture the hole 4 to be marked, so as to achieve the purpose of marking identification. When the micro-tissue is stored, if the micro-tissue is too small, such as a small piece of tissue, eosin can be added dropwise for identification. After the micro tissues are hermetically stored, the embedded bag filled with the micro tissues can be directly subjected to experimental treatment, or the embedded bag filled with the micro tissues is placed into a universal embedded box and is matched with the universal embedded box for use. When the micro tissue needs to be taken out, the micro tissue can be directly taken out by using tools such as pliers, clips and the like after the sealing structure is opened.

To further illustrate the application process of the leakproof embedding bag special for the micro-tissue in this embodiment, the following experimental examples of the "inhibition experiment of the ganoderma lucidum extract and paclitaxel on the 3D breast cancer model" are given:

1. establishment of breast cancer 3D model

MCF-7 adherent cells in a growth phase are selected, digested, supernatant is removed, a 3D culture medium (prepared by taking a complete culture medium as a substrate and adding Matrigel gel with the volume fraction of 0.5%) is added, a cell suspension (1 × 104 cells/hole) is transferred to a 96-hole ultra-low adsorption cell culture plate, and the cell suspension is placed into a carbon dioxide incubator for culture.

2. Dosing

After 48 hours, MCF-7 cells are observed to form compact cell masses, and ganoderma lucidum extracts and paclitaxel with corresponding concentrations are added, wherein the groups comprise blank control A, 50ug/mL of ganoderma lucidum extracts B, 100ug/mL of ganoderma lucidum extracts C, 200ug/mL of ganoderma lucidum extracts D, 200ng/mL of E, 50ug/mL of ganoderma lucidum extracts F and 200ng/mL of paclitaxel, 100ug/mL of ganoderma lucidum extracts G and 200ng/mL of paclitaxel, and 200ug/mL of ganoderma lucidum extracts H and 200ng/mL of paclitaxel.

3. Fixing, dehydrating and embedding

After 48h, fixing the cells on a 96-well plate by using 4% paraformaldehyde for 1h, lightly taking out the cell balls in the 96-well plate by using an earpick, putting the cell balls into the special leakproof embedding bag for the micro tissue, sealing the special leakproof embedding bag for the micro tissue, writing groups and serial numbers on a blank area of a marked area by using a pencil, and putting the cell balls into corresponding embedding boxes according to the groups.

Then the mixture is put on an intelligent biological tissue dehydrator for re-fixation (4% paraformaldehyde is fixed for 1h), dehydration (85% alcohol for 1h, 95% alcohol for 2h, absolute alcohol for 1h, absolute alcohol for 2h and absolute alcohol for III 2h), transparency (environment-friendly transparent dewaxing liquid for 1h and environment-friendly transparent dewaxing liquid for II 1h) and wax dipping (wax for 1h, wax for 1.5h and wax for III 1 h).

And transferring the sample into a wax jar of an embedding machine after the wax dipping is finished, opening an embedding box and an embedding bag one by one, and arranging the cell spheres in a mould in sequence for embedding.

4. Slicing and staining

Slicing at 3 μm thickness, baking at 60 deg.C for 2 hr, and HE dyeing with automatic dyeing machine.

The present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents and equivalents thereof, which are intended to be included in the scope of the present invention.

Claims (10)

1. A special leak-proof embedding bag for micro tissues is characterized by comprising a bag body and a sealing structure arranged on the bag body; wherein, one side of the bag body is provided with an opening to form a taking and placing opening for taking and placing the micro tissues; the sealing structure is arranged at the corresponding position of the taking and placing opening.

2. The leakproof embedding bag special for the tiny tissues as claimed in claim 1, wherein the sealing structure is a clip chain self-sealing strip structure, which comprises a rib and a groove, the rib and the groove are respectively disposed on two inner sides of the bag body.

3. The special leakproof embedding bag for the tiny tissues according to claim 2, wherein the sealing structure is arranged at the inner side of the opening edge of the taking and placing opening; the sealing structure divides the bag body into a storage area and a marking area, wherein the storage area is formed between the sealing structure and the bottom of the bag body, and the marking area is formed between the sealing structure and the edge of the taking and placing opening.

4. The special leakproof embedding bag for the tiny tissues as claimed in claim 3, wherein the marked area comprises a blank area and a hole marked area, and the hole marked area is provided with a plurality of holes to be marked which can be punctured as marks.

5. The special leakproof embedding bag for the tiny tissues according to claim 4, wherein 9 holes to be marked are provided.

6. The leakage-proof embedding bag special for the tiny tissues as claimed in any one of claims 3-5, wherein the size of the bag body is 1cm x 1 cm.

7. The leakproof embedding bag special for the tiny tissue as claimed in claim 6, wherein the size of the marking zone is 1cm x 0.4 cm.

8. The leakproof embedding bag for the micro-tissue as claimed in claim 1, wherein the bag body is made of a filter membrane material.

9. The leakproof embedding bag special for the tiny tissues as claimed in claim 8, wherein the bag body is made of a cellulose acetate filter screen.

10. The leakproof embedding bag special for the tiny tissues as claimed in claim 9, wherein the mesh number of the cellulose acetate filter screen is 100 meshes.

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