CN217007842U

CN217007842U - Optical system and three-dimensional imaging system

Info

Publication number: CN217007842U
Application number: CN202220895339.2U
Authority: CN
Inventors: 汪远; 孙腾骞; 时雷; 李东
Original assignee: Nanjing Weina Technology Research Institute Co ltd
Current assignee: Nanjing Weina Technology Research Institute Co ltd
Priority date: 2022-04-18
Filing date: 2022-04-18
Publication date: 2022-07-19
Anticipated expiration: 2032-04-18

Abstract

The utility model provides an optical system and a three-dimensional imaging system, and relates to the technical field of optical microscopic imaging. The method is used for solving the problems that the existing three-dimensional imaging system is complex in structure, scans a target sample for a long time and damages the cell structure of the target sample by strong light irradiation. The optical system comprises a first objective lens, an optical component, a second objective lens, a reflecting device and a driving device, wherein the reflecting device is used for reflecting the second imaging light beam to form a third imaging light beam; the optical component is configured to guide the first imaging light beam to the second objective lens and to project the fourth imaging light beam to the first imaging device to form a first predetermined planar image of the target sample; the driving device is used for driving the reflecting device to rotate around the optical axis of the second objective lens so as to form different first preset plane images. Different first predetermined plane images can be obtained quickly by arranging the driving device, the structure is simple, the efficiency is improved, the cost is reduced, and the histiocyte of the target sample is protected.

Description

Optical system and three-dimensional imaging system

Technical Field

The utility model relates to the technical field of optical microscopic imaging, in particular to an optical system and a three-dimensional imaging system.

Background

The three-dimensional optical microscopic imaging of the biological target sample has extremely important functions and significance in scientific research, pathological diagnosis and other scientific research and application fields.

Three-dimensional optical imaging usually adopts confocal microscopy, and each plane of biological tissues needs to be scanned from two directions, and the plane perpendicular to the axial direction is imaged first, and then the axial direction is moved and scanned, so that three-dimensional imaging is realized. In moving scanning in the axial direction, a plurality of high-precision motor drives are required to three-dimensionally move a target sample. The three-dimensional optical imaging can also adopt an optical sheet technology, the optical sheet technology combines two mutually orthogonal different optical paths, one optical path is used for rapid detection of a wide field of view, the other optical path is used for illumination through a thin optical sheet, and the used optical structure and mechanical structure are complex.

When the confocal microscopy technology is adopted for three-dimensional optical imaging, point-by-point scanning is needed for imaging a target sample at a specific depth, the time efficiency is low, the target sample is easily bleached due to long-time scanning, and cells or tissue structures are easily damaged due to strong light irradiation. During the scanning process, a plurality of motor drives are required, and the complexity and the cost of the structure are increased. The microscope complete machine adopted by the light sheet technology is expensive, and the economic cost is high. And the microscope adopted by the optical sheet technology records all the information of the biological cells in the visual field, and the adopted data algorithm is difficult during the post-processing, so that the requirement on a corresponding data processing device is high.

SUMMERY OF THE UTILITY MODEL

In view of the above problems, embodiments of the present invention provide an optical system and a three-dimensional imaging system, which simplify the structure and reduce the cost, and improve the efficiency.

In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:

a first aspect of embodiments of the present invention provides an optical system.

An optical system comprising a first objective lens, an optical component, a second objective lens, a reflecting device and a driving device, wherein:

the first objective lens is used for imaging a target sample to obtain a first imaging light beam;

the second objective lens is used for receiving the first imaging light beam to generate a second imaging light beam and receiving a third imaging light beam reflected by the reflecting device to generate a fourth imaging light beam;

the reflecting surface of the reflecting device and the optical axis of the second objective lens form a preset angle theta and are used for reflecting the second imaging light beam to form a third imaging light beam;

the optical component is configured to direct the first imaging beam into the second objective lens and project the fourth imaging beam to a first imaging device to form a first predetermined planar image of the target sample;

the driving device is used for driving the reflecting device to rotate around the optical axis of the second objective lens so as to form different first preset plane images.

Compared with the prior art, the optical system provided by the embodiment of the utility model has the following advantages:

the optical system is provided with a driving device for driving the reflecting device to rotate, the driving device is arranged for driving the reflecting device to rotate around the optical axis of the second objective, when the reflecting device rotates around the optical axis of the second objective, the reflecting surface of the reflecting device reflects to obtain different third imaging light beams, and different first preset plane images are obtained through the second objective and the optical component. Different first preset plane images can be obtained by arranging a driving device to drive a reflecting device to rotate, three-dimensional images of a target sample can be synthesized by utilizing the first preset plane images, point-by-point scanning and complex mechanism action are not needed, and the device is simple in structure and high in efficiency.

As an improvement of the optical system according to the embodiment of the present invention, the driving device includes a fixed portion and a rotating portion rotatably connected to the fixed portion, a rotation axis of the rotating portion coincides with an optical axis of the second objective lens, and the reflecting device is fixed to the rotating portion.

As a modification of the optical system according to the embodiment of the present invention, the fixed portion includes an outer race, and the rotating portion includes the inner race.

As an improvement of the optical system according to the embodiment of the present invention, the rotating portion further includes an end cap disposed at one end of the bearing inner race, and the reflection device is fixed to an inner side surface of the end cap.

As an improvement of the optical system in the embodiment of the present invention, the end cap includes a cover plate and a position-limiting sleeve disposed inside the cover plate, the position-limiting sleeve is engaged with the bearing inner ring, and the reflection device is fixed on an inner side surface of the cover plate.

As an improvement of the optical system in the embodiment of the present invention, a limiting protrusion is disposed on an inner side of the end cap, and a limiting surface for cooperating with the limiting protrusion for limiting is disposed on the reflection device.

As an improvement of the optical system according to the embodiment of the present invention, the reflection device is in a shape of a straight triangular prism, the limiting protrusion includes two limiting strips arranged in parallel to each other, the limiting surface includes two bottom surfaces of the reflection device, and the two bottom surfaces are respectively matched with the two limiting strips.

As an improvement of the optical system according to the embodiment of the present invention, the driving device further includes a transmission mechanism and a motor disposed on the fixing portion, and the motor is configured to drive the rotating portion to rotate through the transmission mechanism.

As an improvement of the optical system of the embodiment of the utility model, the transmission mechanism comprises a driving gear and a first driven gear meshed with the driving gear,

the driving gear is in transmission connection with a motor shaft of the motor;

the first driven gear is connected with the rotating part, and the axis of the first driven gear coincides with the optical axis of the second objective lens.

As an improvement of the optical system according to the embodiment of the present invention, the bearing inner race of the rotating portion includes an overhang portion, the overhang portion extends out of the bearing outer race of the fixing portion in the axial direction, and the first driven gear is sleeved outside the overhang portion.

As an improvement of the optical system according to the embodiment of the present invention, the transmission mechanism further includes at least one second driven gear provided on the fixing portion, the second driven gear is engaged with the first driven gear, and the second driven gear and the driving gear are arranged along a circumferential direction of the first driven gear.

As an improvement of the optical system according to the embodiment of the present invention, the motor is a stepping motor or a servo motor.

As an improvement of the optical system according to the embodiment of the present invention, the driving device further includes a leveling structure for leveling the fixing portion.

As a modification of the optical system according to the embodiment of the present invention, the rotating portion includes a weight structure for balancing the weight of the reflecting device.

As a refinement of the optical system according to an embodiment of the utility model, the optical component is further configured to project the first imaging beam to a second imaging device to form a second predetermined planar image of the target specimen.

As an improvement of the optical system according to the embodiment of the present invention, the optical component further includes a first beam splitter disposed downstream of the first objective optical path and a second beam splitter disposed upstream of the second objective optical path, a first lens and a second lens are disposed between the first beam splitter and the second beam splitter, and the first imaging light beam is reflected by the first beam splitter, passes through the first lens and the second lens, and is guided to the second objective lens by reflection by the second beam splitter.

As an improvement of the optical system according to the embodiment of the utility model, the predetermined angle is any value between 0 and 90 degrees.

As a modification of the optical system according to the embodiment of the present invention, the predetermined angle is 45 degrees.

A second aspect of embodiments of the present invention provides a three-dimensional imaging system.

A three-dimensional imaging system includes the optical system as described above, and further includes a first imaging device, a data acquisition device, and a data processing device, where the data acquisition device is connected to both the first imaging device and the data processing device, and the first imaging device is configured to provide the first imaging plane and generate first image information of the first predetermined plane image; the data acquisition device is used for acquiring the first image information and sending the first image information to the data processing device; the data processing device is used for generating three-dimensional image information of the target sample at least according to the first image information.

Compared with the prior art, the three-dimensional imaging system provided by the embodiment of the utility model has the following advantages:

the three-dimensional imaging system has the advantages of simple structure and low cost due to the adoption of the optical system, can quickly synthesize the three-dimensional image of the target sample without point-by-point scanning, has high efficiency, and avoids damaging cells or tissue structures by strong light irradiation.

As an improvement of the three-dimensional imaging system according to the embodiment of the present invention, the three-dimensional imaging system further includes a second imaging device connected to the data acquisition device, the second imaging device being configured to provide a second imaging plane and generate second image information of a second predetermined plane image; the data acquisition device is also used for acquiring the second image information and sending the second image information to the data processing device; the data processing device is used for generating three-dimensional image information of the target sample according to the first image information and the second image information.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural diagram of a three-dimensional imaging system provided by an embodiment of the utility model;

FIG. 2 is a schematic diagram of an optical system according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a driving device according to an embodiment of the present invention;

FIG. 4 is a front view of a drive unit provided in accordance with an embodiment of the present invention;

fig. 5 is a sectional view of a driving device according to an embodiment of the present invention.

Description of reference numerals:

100: a three-dimensional imaging system; 102: a first imaging device; 103: a second imaging device; 104: a data acquisition device; 105: a data processing device; 101: an optical system; 3: a reflecting device; 4: a drive device; 411: a bearing inner race; 412: a bearing outer race; 42: an end cap; 421: a cover plate; 422: a limiting strip; 43: a driving gear; 44: a first driven gear; 45: a second driven gear; 46: a fixed part; 47: a motor; 1: a first objective lens; 2: a second objective lens; 5: a first lens; 6: a second lens; 7: a third lens; 8: a fourth lens; 9: a first light splitting element; 10: a second light splitting element; 11: a first light source; 12: a second light source; 13: a third light source; 14: a target sample; 15: a first predetermined plane; 16: a second predetermined plane.

Detailed Description

The three-dimensional optical microscopic imaging has extremely important functions and significance in scientific research, pathological diagnosis and other scientific research and application fields. Most of microscopic three-dimensional imaging adopts a confocal microscope, a two-photon microscope or a multi-photon microscope, a target sample is scanned and collected point by point to form images under different depths, a plane perpendicular to the axial direction is imaged firstly, then the plane is moved along the axial direction, another plane perpendicular to the axial direction is imaged, the process is repeated to carry out moving scanning on the axial direction, and therefore three-dimensional imaging is achieved. When moving scanning is performed in the axial direction, a plurality of high-precision motors are needed to drive the target sample to perform three-dimensional movement, which increases the complexity and cost of the structure; point-by-point scanning is needed for imaging a specific depth of a target sample, and time efficiency is low; long scans tend to cause bleaching of the target sample and intense light exposure tends to damage cells or tissue structures.

In order to solve the technical problems, the utility model provides an optical system and a three-dimensional imaging system. The driving device is arranged in the optical system, the reflecting device is driven to rotate around the optical axis of the second objective, when the reflecting device rotates around the optical axis of the second objective, the reflecting surface of the reflecting device reflects to obtain different third imaging light beams, the different first preset plane images are obtained through the second objective and the optical component, the three-dimensional images of the target sample can be synthesized by utilizing the first preset plane images, point-by-point scanning and complex mechanism action are not needed, the structure is simple, the efficiency of generating the three-dimensional images is high, and damage to cells or tissue structures caused by strong light irradiation is avoided.

In order to make the aforementioned objects, features and advantages of the embodiments of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. It is to be understood that the described embodiments are merely a few embodiments of the utility model, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the drawings, the sizes of the respective constituent elements are not reflected in the original actual sizes, but are enlarged for convenience of explanation and understanding.

A three-dimensional imaging system according to an embodiment of the present invention is described below with reference to the drawings.

Referring to fig. 1 and fig. 2, fig. 1 is a schematic structural diagram of a three-dimensional imaging system according to an embodiment of the present invention, and fig. 2 is a schematic structural diagram of an optical system according to an embodiment of the present invention.

The three-dimensional imaging system 100 includes an optical system 101, a first imaging device 102, a data acquisition device 104, and a data processing device 105, wherein the data acquisition device 104 is connected to both the first imaging device 102 and the data processing device 105. The first imaging device 102 is configured to provide a first imaging plane for the optical system 101 and generate first image information, the data acquisition device 104 is configured to acquire the first image information and send the first image information to the data processing device 105, and the data processing device 105 is configured to generate three-dimensional image information (described in detail later) of the target sample 14 according to at least the first image information.

An optical system according to an embodiment of the present invention is described below with reference to the drawings.

Referring to fig. 2 and 5, fig. 2 is a schematic structural diagram of an optical system according to an embodiment of the present invention, and fig. 5 is a cross-sectional view of a driving apparatus according to an embodiment of the present invention. The optical system 101 comprises a first objective lens 1, optical components, a second objective lens 2, a reflecting device 3 and a driving device 4, wherein: the first objective lens 1 is used for imaging the target sample 14 to obtain a first imaging light beam; the second objective lens 2 is used for receiving the first imaging light beam to generate a second imaging light beam and receiving the third imaging light beam reflected by the reflecting device 3 to generate a fourth imaging light beam; the reflecting surface of the reflecting device 3 and the optical axis of the second objective lens 2 form a preset angle and are used for reflecting the second imaging light beam to form a third imaging light beam; the optical components are configured to direct the first imaging beam into the second objective 2 and to form the fourth imaging beam into a first predetermined planar image of the target sample 14; the driving device 4 is used for driving the reflecting device 3 to rotate around the optical axis of the second objective lens 2 so as to form different first predetermined plane images.

The first objective lens 1 and the second objective lens 2 may be high power objective lenses or low power objective lenses.

In an alternative embodiment, referring to fig. 2 and 5, the optical component comprises: a first light splitting element 9, a first lens 5, a second lens 6, a second light splitting element 10, and a third lens 7. The first light splitting element 9 is arranged on the side of the first objective lens 1 facing away from the target specimen 14, the first lens 5 is arranged on the side of the first light splitting element 9, the second lens 6 is arranged on the side of the first lens 5 facing away from the first light splitting element 9, the second light splitting element 10 is arranged on the side of the second objective lens 2 facing away from the driving device 4, and the third lens 7 is arranged on the side of the second light splitting element 10 facing away from the second objective lens 2.

In the above embodiment, light emitted from the light source and passing through the target sample 14, or fluorescence emitted from the target sample 14 after being illuminated by the light source enters the first objective lens 1 to form a first imaging light beam, the first imaging light beam is reflected by the first beam splitter 9, passes through the first lens 5 and the second lens 6, is reflected by the second beam splitter 10 to enter the second objective lens 2, is reflected by the reflecting device 3 to form a third imaging light beam, and then passes through the second objective lens 2 to generate a fourth imaging light beam, and the fourth imaging light beam passes through the second beam splitter 10 and the third lens 7 to form a first predetermined planar image of the target sample 14.

Wherein the light source may comprise only the first light source 11 or only the second light source 12. In a specific embodiment, to enhance the lighting effect, the light source may comprise a first light source 11, a second light source 12 and a third light source 13. The first light source 11 is arranged on the side of the target sample 14 facing away from the first objective lens 1, the second light source 12 is arranged on the periphery of the first objective lens 1 and near the target sample 14, and the third light source 13 is arranged on the side of the first light splitting element 9 facing away from the first objective lens 1.

The optical system 101 provided by the embodiment of the present invention can rapidly obtain a plurality of specific plane images of the target sample 14 without scanning the target sample 14 for a long time. Specifically, after the target sample 14 is irradiated by the light source (the first light source 11, the second light source 12, and/or the third light source 13), the first imaging light beam is formed to record three-dimensional information of the target sample 14, the three-dimensional information includes image information of a first predetermined plane 15 of the target sample 14, an included angle between the reflection surface of the reflection device 3 and the first predetermined plane 15 is a predetermined angle θ, and 0 ° < θ <90 °, that is, the predetermined angle may be any value between 0 and 90 degrees. Preferably, θ is 45 degrees. The first imaging light beam is received by the first objective lens 1 and illuminates the first light splitting element 9. In the light irradiated to the first light splitting element 9, information of the first predetermined plane 15 of the target specimen 14 is described along the transmission direction of the light. The light is reflected by the first beam splitting element 9 and reaches the second beam splitting element 10 through the first lens 5 and the second lens 6, respectively. The light reflected by the second light splitting element 10 passes through the second objective lens 2 to generate a second imaging light beam to irradiate the reflecting device 3, the reflecting surface of the reflecting device 3 reflects the light to generate a third imaging light beam and irradiates the third imaging light beam back to the second objective lens 2, and the third imaging light beam is received by the second objective lens 2 to generate a fourth imaging light beam to irradiate the second light splitting element 10. In this portion of light, the perpendicular light transmission direction describes information of the first predetermined plane 15 of the target specimen 14, and the position of the first predetermined plane 15 in the target specimen 14 corresponds one-to-one to the mounting position of the reflection device 3. The light irradiated from the second objective lens 2 passes through the second spectroscopic element 10 and reaches the first imaging device 102, and since the first imaging device 102 recognizes that the light information is vertical to the light transmission direction and the vertical light transmission direction at this time describes the information of the first predetermined plane 15 of the target specimen 14, the first imaging device 102 recognizes and displays the information of the first predetermined plane 15 of the target specimen 14 (i.e., the first predetermined plane image).

Since one determined position of the reflecting means 3 corresponds to information of one determined position of the first predetermined plane 15 in the target specimen 14, i.e. information of the first predetermined plane 15 of the target specimen 14 is identified, displayed and recorded by the first imaging means 102. At this time, the reflecting device 3 rotates with the optical axis of the second objective lens 2 as a rotation axis, and the first imaging device 102 sequentially records information of each first predetermined plane 15 of the target specimen 14 corresponding to the position of the reflecting device 3. Three-dimensional reconstruction of these data enables three-dimensional imaging of the target sample 14.

The driving device 4 rotates around the optical axis of the second objective lens 2 by driving the reflecting device 3, the second imaging light beam forms different third imaging light beams through the reflecting device 3, and different first predetermined plane images can be quickly formed through other optical components, so that the efficiency is high, the structure is simple, and the cost is low.

The drive device 4 can be any device that meets the requirements, such as a direct motor drive. To facilitate the installation of the driving device 4, it is preferable that the driving device 4 includes a fixed portion 46 and a rotating portion rotatably connected to the fixed portion 46, the axis of rotation of the rotating portion coincides with the optical axis of the second objective lens 2, and the reflecting device 3 is fixed to the rotating portion.

The fixing portion 46 and the rotating portion may be configured to be any portion that can satisfy the requirement that the reflecting device 3 can rotate around the optical axis of the second objective lens 2, for example, a connection manner of a sleeve and a shaft is directly selected, the sleeve is configured as the fixing portion, and the shaft is configured as the rotating portion. In order to simplify the structure and reduce the cost, and ensure the accuracy of the rotation, it is preferable that the fixing portion 46 includes a housing and a bearing outer race 412 mounted on the housing, and the rotating portion includes a bearing inner race 411. The bearing can be a deep groove ball bearing, a rolling bearing, a needle bearing and the like, and has the advantages of low motion friction coefficient, small friction force, low abrasion and good precision.

In order to facilitate the installation of the reflection unit 3 and ensure the rotation accuracy of the reflection unit 3 around the optical axis of the second objective lens 2, the rotation part preferably further includes an end cap 42 disposed at one end of the bearing inner ring 411, and the reflection unit 3 is fixed on the inner side surface of the end cap 42. When the bearing inner race 411 rotates, the reflection device 3 also follows the rotation. In this way, the rotational accuracy of the reflecting device 3 can be ensured without adding other devices.

In order to facilitate the positioning of the end cover 42 and the bearing inner race 411, the end cover 42 includes a cover plate 421 and a position limiting sleeve disposed inside the cover plate 421, the position limiting sleeve is matched with the bearing inner race 411, and the reflection device 3 is fixed on the inner side surface of the cover plate 421. The end cover and the bearing inner ring are positioned by the limiting sleeve, so that the structure is simple and the positioning is accurate.

The positioning mode of the reflecting device 3 is not limited, and the reflecting device can be positioned by a clamp and a hole. Preferably, in order to accurately position the reflection apparatus 3 on the cover plate 421, a limiting protrusion is provided on the inner side of the end cover 42, and a limiting surface for matching with the limiting protrusion for limiting is provided on the reflection apparatus 3.

The limiting protrusion may be configured to have any structure that can meet the requirement, and in order to position the reflection apparatus 3, preferably, in a specific embodiment, referring to fig. 4 and 5, fig. 4 is a front view of a driving apparatus provided in an embodiment of the present invention, and fig. 5 is a cross-sectional view of the driving apparatus provided in an embodiment of the present invention. The limiting protrusion comprises two limiting strips 422 which are arranged in parallel, the reflecting device 3 is in a straight triangular prism shape, the limiting surface comprises two bottom surfaces of the reflecting device 3, and the two bottom surfaces are respectively matched with the two limiting strips 422. The accuracy of the positioning of the reflecting means 3 can be ensured by the surface positioning.

In order to better drive the rotation of the reflection device 3 and achieve the compactness of the structure, preferably, in a specific embodiment, referring to fig. 3, fig. 3 is a structural schematic diagram of the driving device provided by the embodiment of the present invention, the driving device 4 further includes a transmission mechanism and a motor 47 disposed on the fixed portion 46, the motor 47 is preferably mounted on the housing of the fixed portion 46, and the motor 47 is used for driving the rotation portion to rotate through the transmission mechanism.

The transmission mechanism can be set to any structure capable of meeting the requirements, such as gear transmission, chain transmission and worm and gear transmission. In order to ensure the accuracy and facilitate the conversion of the rotation angle, it is preferable that the transmission mechanism includes a driving gear 43 and a first driven gear 44 engaged with the driving gear 43, the driving gear 43 is connected with a motor shaft of the motor 47; the first driven gear 44 is connected to the rotating portion, and the axis of the first driven gear 44 coincides with the optical axis of the second objective lens 2.

For the sake of compactness and ease of installation of the first driven gear 44, it is preferable that, in one specific embodiment, the bearing inner race 411 of the rotating portion includes an overhang portion extending axially beyond the bearing outer race 412 of the fixing portion 46, and the first driven gear 44 is fitted outside the overhang portion.

In order to ensure the rotational smoothness of the first driven gear 44, in a further preferred embodiment, the transmission mechanism further includes at least one second driven gear 45 provided on the fixed portion 46, the second driven gear 45 is mounted on a transmission shaft of a housing of the fixed portion 46, the second driven gear 45 is engaged with the first driven gear 44, and the second driven gear 45 and the driving gear 43 are arranged along a circumferential direction of the first driven gear 44. Preferably, in a specific embodiment, referring to fig. 3, fig. 3 is a schematic structural diagram of a driving device provided by the embodiment of the present invention, the transmission mechanism includes 2 second driven gears 45, 2 second driven gears 45 are respectively engaged with the first driven

gear

44, and 2 second driven gears 45 and the driving gear 43 are arranged along a circumferential direction of the first driven gear 44. Of course, it is understood that the number of the driven gears 45 may be 1, 3 or more.

The type of the motor 47 is not limited, and for simplicity of control and accuracy assurance, the motor 47 is preferably a stepping motor or a servo motor.

In order to level the bearing outer race 412, a leveling structure is provided on the drive device 4. The leveling structure can be any structure that can meet the requirement, and in a specific embodiment, a leveling screw is disposed on the housing of the fixing portion 46, the leveling screw connects the housing of the fixing portion 46 and the bearing outer ring 412, a thread is disposed at a position where the housing and the bearing outer ring 412 correspond to each other, and whether the central axis of the bearing outer ring 412 coincides with the optical axis of the second objective lens 2 is adjusted by the adjusting screw.

In order to balance the weight of the reflection means 3, a counterweight structure is provided on the drive means 4. The counterweight structure can be configured to be any structure that can meet the requirement, and for the convenience of installation of the counterweight structure, it is preferable that a square block is disposed on the cover plate 421 to balance the weight of the reflection device 3 in a specific embodiment.

In the three-dimensional imaging system 100, the first imaging device 102 provides a first imaging plane, the optical component projects the fourth imaging beam to the first imaging device 102 to form an image of a first predetermined plane, the driving device 4 drives the reflection device to rotate, so as to obtain first predetermined plane images of different angles, and the first predetermined plane images are synthesized, so that a three-dimensional image of the target sample can be fitted.

The embodiment of the utility model also provides a three-dimensional imaging method, which comprises the following steps: rotating the reflecting device 3 and collecting a plurality of first predetermined plane images in the rotating process; image synthesis is performed on the plurality of first predetermined planar images to obtain a three-dimensional image of the target sample 14. When the first predetermined planar image is acquired, the acquisition may be performed once every time the reflecting device 3 rotates by a predetermined angle, or may be performed once every time a predetermined time elapses.

Referring to fig. 1, the three-dimensional imaging method employs a three-dimensional imaging system 100 of a first imaging device 102. It is to be understood that the three-dimensional imaging method provided by the embodiment may also be applied to other three-dimensional imaging systems, if the requirements are met.

In another alternative embodiment, the three-dimensional imaging system 100 further comprises a second imaging device 103 connected to the data acquisition device 104, the second imaging device 103 being configured to provide a second imaging plane and generate second image information; the data acquisition device 104 is further configured to acquire second image information and send the second image information to the data processing device 105; the data processing device 105 is configured to generate three-dimensional image information of the target specimen 14 from the first image information and the second image information.

In the above-described embodiment, after the target sample 14 is irradiated by the light source (the first light source 11, the second light source 12 and/or the third light source 13), the first imaging light beam is formed to record the three-dimensional information of the target sample 14, the three-dimensional information includes the image information of the first predetermined plane 15 and the second predetermined plane 16 of the target sample 14, the angles between the reflection surface of the reflection device 3 and the first predetermined plane 15 and the second predetermined plane 16 are respectively the predetermined angle θ, 90 ° - θ, and 0 ° ≦ θ ≦ 90 °, that is, the predetermined angle may be any value between 0 ° and 90 °, including 0 ° and 90 °. Preferably, θ is 45 °. When θ is 0 ° and 90 °, the three-dimensional imaging system is degraded to a three-dimensional imaging system that obtains a three-dimensional image of the target specimen 14 by image-synthesizing different second predetermined plane images. The first imaging beam is received by the first objective lens 1 and directed to the first light splitting element 9. Of the light irradiated to the first light splitting element 9, information of the first predetermined plane 15 of the target sample 14 is described along the transmission direction of the light, and information of the second predetermined plane 16 of the target sample 14 is described perpendicular to the transmission direction of the light. The light is reflected by the first beam splitting element 9 and then reaches the second beam splitting element 10 through the lens 5 and the lens 6, respectively.

The light reflected by the second light splitting element 10 passes through the second objective lens 2 to generate a second imaging light beam to irradiate the reflecting device 3, the reflecting surface of the reflecting device 3 reflects the light to generate a third imaging light beam and irradiates the third imaging light beam back to the second objective lens 2, and the third imaging light beam is received by the second objective lens 2 to generate a fourth imaging light beam to irradiate the second light splitting element 10. In this part of the light, the vertical light transmission direction records information of the first predetermined plane 15 of the target specimen 14, and the position of the first predetermined plane 15 in the target specimen 14 corresponds to the installation position of the reflection device 3 one by one. The light irradiated from the second objective lens 2 passes through the second spectroscopic element 10 and reaches the first imaging device 102, and since the first imaging device 102 recognizes that the light information is the light information of the vertical light transmission direction and the vertical light transmission direction at this time describes the information of the first predetermined plane 15 of the target specimen 14, the first imaging device 102 recognizes and displays the information of the first predetermined plane 15 of the target specimen 14 (i.e., the first predetermined plane image). Since a determined position of the reflecting means 3 corresponds to information of a determined position of the first predetermined plane 15 in the target specimen 14, i.e. information of the first predetermined plane 15 of the target specimen 14 is identified, displayed and recorded by the first imaging means 102. At this time, the reflecting device 3 rotates with the optical axis of the second objective lens 2 as a rotation axis, and the first imaging device 102 sequentially records information of each first predetermined plane 15 of the target specimen 14 corresponding to the position of the reflecting device 3.

The light transmitted through the second spectroscopic element 10 passes through the fourth lens 8 to reach the second imaging device 103. Since the second imaging device 103 recognizes that the light information of the vertical light transmission direction is present, and the transmission direction of the vertical light at this time describes the information of the second predetermined plane 16 of the target specimen 14, the second imaging device 103 recognizes and displays the information of the second predetermined plane 16 of the target specimen 14 (i.e., the aforementioned second predetermined plane image).

The information of the first predetermined plane 15 and the information of the second predetermined plane 16 are three-dimensionally reconstructed to achieve three-dimensional imaging of the target specimen 14.

In another alternative embodiment, a three-dimensional imaging method includes: rotating the reflecting device 3, and acquiring a second preset plane image and a plurality of first preset plane images in the rotating process; and synthesizing the second predetermined plane image and the plurality of first predetermined plane images to obtain a three-dimensional image of the target sample 14. It is understood that the three-dimensional imaging method provided by the embodiment can also adopt other three-dimensional imaging systems under the condition of meeting the requirement.

In the present specification, each embodiment or implementation mode is described in a progressive manner, and the emphasis of each embodiment is on the difference from other embodiments, and the same and similar parts between the embodiments may be referred to each other.

In the description of the present specification, references to "one embodiment", "some embodiments", "an illustrative embodiment", "an example", "a specific example", or "some examples" or the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An optical system comprising a first objective lens, an optical component, a second objective lens, a reflecting device and a driving device, wherein:

the second lens is used for receiving the first imaging light beam to generate a second imaging light beam and receiving a third imaging light beam reflected by the reflecting device to generate a fourth imaging light beam;

2. The optical system according to claim 1, wherein the driving device includes a fixed portion and a rotating portion rotatably connected to the fixed portion, a rotation axis of the rotating portion coincides with an optical axis of the second objective lens, and the reflecting device is fixed to the rotating portion.

3. The optical system of claim 2, wherein the fixed portion comprises an outer race and the rotating portion comprises the inner race.

4. The optical system of claim 3, wherein the rotating portion further comprises an end cap disposed at one end of the bearing inner race, and the reflection device is fixed to an inner side surface of the end cap.

5. The optical system of claim 4, wherein the end cap comprises a cover plate and a stop collar disposed inside the cover plate, the stop collar engaging the inner race, the reflective device being secured to an inner side of the cover plate.

6. The optical system according to claim 4, wherein the inner side of the end cap is provided with a limiting protrusion, and the reflection device is provided with a limiting surface for matching with the limiting protrusion for limiting.

7. The optical system according to claim 6, wherein said reflecting means is in the shape of a right triangular prism, said limiting protrusion comprises two limiting bars arranged in parallel, said limiting surface comprises two bottom surfaces of said reflecting means, and said two bottom surfaces are respectively engaged with said two limiting bars.

8. The optical system according to any one of claims 2 to 7, wherein the driving device further comprises a transmission mechanism and a motor disposed on the fixed portion, and the motor is configured to drive the rotating portion to rotate through the transmission mechanism.

9. The optical system of claim 8, wherein the transmission mechanism includes a driving gear and a first driven gear engaged with the driving gear,

the driving gear is in transmission connection with a motor shaft of the motor;

the first driven gear is connected with the rotating part, and the axis of the first driven gear is overlapped with the optical axis of the second objective lens.

10. The optical system of claim 9, wherein the bearing inner race of the rotating portion includes an overhang portion extending axially beyond the bearing outer race of the fixed portion, the first driven gear being sleeved outside the overhang portion.

11. The optical system of claim 9, wherein the transmission mechanism further comprises at least one second driven gear disposed on the fixed portion, the second driven gear is engaged with the first driven gear, and the second driven gear and the driving gear are arranged along a circumferential direction of the first driven gear.

12. The optical system of claim 8, wherein the motor is a stepper motor or a servo motor.

13. The optical system according to any one of claims 2 to 7, wherein the driving device further comprises a leveling structure for leveling the fixing portion.

14. An optical system as claimed in any one of claims 2 to 7, characterized in that the rotating part comprises a counterweight structure for balancing the weight of the reflecting means.

15. The optical system of any one of claims 1 to 7, wherein the optical assembly is further configured to project the first imaging beam to a second imaging device to form a second predetermined planar image of the target specimen.

16. The optical system according to any one of claims 1 to 7, wherein the optical component further includes a first beam splitter disposed downstream of the first objective optical path and a second beam splitter disposed upstream of the second objective optical path, a first lens and a second lens are disposed between the first beam splitter and the second beam splitter, and the first imaging beam is reflected by the first beam splitter, passes through the first lens and the second lens, and is guided to the second objective lens by reflection by the second beam splitter.

17. The optical system according to any one of claims 1 to 7, wherein the predetermined angle is any value between 0 and 90 degrees.

18. The optical system of claim 17, wherein the predetermined angle is 45 degrees.

19. A three-dimensional imaging system comprising the optical system of any one of claims 1 to 18, further comprising a first imaging device, a data acquisition device, and a data processing device, the data acquisition device being connected to both the first imaging device and the data processing device, wherein,

the first imaging device is used for providing the first imaging surface and generating first image information of the first preset plane image;

the data acquisition device is used for acquiring the first image information and sending the first image information to the data processing device;

the data processing device is used for generating three-dimensional image information of the target sample at least according to the first image information.

20. The three-dimensional imaging system of claim 19, further comprising a second imaging device coupled to the data acquisition device, the second imaging device configured to provide a second imaging plane and generate second image information for a second predetermined planar image;

the data acquisition device is also used for acquiring the second image information and sending the second image information to the data processing device;

the data processing device is used for generating three-dimensional image information of the target sample according to the first image information and the second image information.