CN216998232U

CN216998232U - Special collector of protein purification appearance

Info

Publication number: CN216998232U
Application number: CN202220579920.3U
Authority: CN
Inventors: 张磊; 王宇
Original assignee: Suzhou Ruiheng Jiahang Medical Technology Co ltd
Current assignee: Suzhou Ruiheng Jiahang Medical Technology Co ltd
Priority date: 2022-03-16
Filing date: 2022-03-16
Publication date: 2022-07-19
Anticipated expiration: 2032-03-16

Abstract

The utility model discloses a collecting device of a protein purifier, which comprises a fixed support arranged on the protein purifier, wherein a protein fraction collecting channel is arranged on the outer side of the fixed support, the protein fraction collecting channel is provided with a plurality of three-way switching valves which are sequentially and transversely distributed and are respectively connected with a control module of the protein purifier, liquid inlet ends and liquid outlet ends of two adjacent three-way switching valves are connected through a fraction pipeline, a protein storage box is arranged below the fixed support, a plurality of protein collecting bottles are arranged in the protein storage box, and liquid outlet ends of the three-way switching valves are communicated with the protein collecting bottles through liquid outlet end hoses. The method can automatically collect the protein solution in the protein purification process, can quickly collect the protein in batches, can store the collected protein solution at low temperature, keeps the activity of the protein solution, and is suitable for large-scale preparation of purified protein in industrial production.

Description

Special collector of protein purification appearance

Technical Field

The utility model belongs to the technical field of protein purification equipment, and particularly relates to a special collector for a protein purification instrument.

Background

Protein separation and purification are widely used in biochemical research applications, and are an important separation technology, and a protein purification instrument is generally adopted for protein purification operation, and when the protein purification instrument is used for purifying proteins, a special collector is required to be used for collecting purified protein liquid.

The collection device of current protein purification appearance sets up at the structure, service function is single, when carrying out protein fraction and collecting, once only can place a receiving flask, collects and has filled up the replacement and just need suspend and change the receiving flask, and it is difficult to take trouble, needs the staff to operate at the scene constantly to the unable quick large-scale production that is applicable to protein of this kind of conventional protein liquid collection device has brought certain puzzlement in the factory production of reality.

Disclosure of Invention

The utility model aims to provide a special collector for a protein purification instrument aiming at the problems.

In order to achieve the purpose, the utility model adopts the following technical scheme: the utility model provides a special collector of protein purification appearance, is including installing the fixed bolster on protein purification appearance, its characterized in that, the fixed bolster outside be equipped with albumen fraction collection passageway, just albumen fraction collection passageway have a plurality of transverse distribution in proper order set up and respectively with the three-way switch valve that the control module of protein purification appearance links to each other, link to each other through the fraction pipeline between the liquid business turn over end of two adjacent three-way switch valves, be equipped with the protein storage box below the fixed bolster, be equipped with a plurality of transverse distribution in proper order set up and respectively with the protein receiving flask of three-way switch valve one-to-one in the protein storage box, just three-way switch valve's play liquid end communicate with the protein receiving flask through going out liquid end hose.

In foretell special collector of protein purification appearance, the fixed bolster include the collection passageway installation department of vertical setting, just protein fraction collection passageway install in collection passageway installation department surface, collection passageway installation department upper end have the fixed part of inside buckling just collection passageway installation department and fixed part between be the setting of L shape, the fixed part pass through a plurality of screw fixed mounting on protein purification appearance.

In the above-mentioned special collector of protein purification appearance, the fixed bolster be the corrosion resistant plate material, collection passageway installation department and fixed part even formula structure as an organic whole.

In the collector special for the protein purification instrument, the three-way switching valves are electric three-way valves or pneumatic three-way valves, and the three-way switching valves are 12-24 and are sequentially and uniformly distributed at equal intervals.

In the special collector for the protein purifier, the fraction pipeline is a stainless steel pipe, the fraction pipeline and the three-way switching valve are sequentially connected in series to form a liquid channel transversely penetrating through the fixed support, and the liquid outlet end hose is respectively connected with the liquid channel.

In the above collector special for the protein purification instrument, the liquid outlet end hose is made of rubber, and the liquid outlet end hose is vertically arranged, and the lower end of the liquid outlet end hose is positioned above the protein collection bottle or extends into the protein collection bottle.

In the above collector dedicated for protein purificator, the protein storage tank is a low-temperature storage type storage tank, and the protein collection bottles are sequentially detachably arranged on a tray in the protein storage tank.

In the special collector for the protein purification instrument, the upper end of the tray is provided with a placing surface, the placing surface is provided with a plurality of placing grooves, and the lower end of the protein collecting bottle is placed in the placing grooves.

In foretell special collector of protein purification appearance, the tray in have an inner chamber, the inner chamber of tray in be equipped with a plurality of and place the semiconductor refrigeration piece of recess one-to-one, just the refrigeration face of semiconductor refrigeration piece pass through the bonding mode and place the recess inboard and link to each other.

In the above-mentioned special collector of protein purification appearance, the fraction pipeline be horizontal distribution setting and each fraction pipeline all coaxial setting, just the fraction pipeline pass through welding mode or bonding mode and connect on collecting the passageway installation department.

Compared with the prior art, the utility model has the advantages that: on firmly being fixed in protein purification appearance with protein liquid pipeline through corrosion resistant plate, adopt three-way switch valve to connect protein fraction pipeline, can effectual collection batch purification's protein fraction, the collecting flask can also replace after having been full, the protein storage box has the low temperature storage function, the influence of effectual reduction temperature, the collecting flask in the protein storage box can also replace, three-way switch valve can circulate and switch to satisfy the protein that the mill needs a large amount of collections.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a structural sectional view of a fixing bracket according to the present invention;

FIG. 3 is a schematic diagram of a pneumatic three-way valve of the present invention;

FIG. 4 is a schematic diagram of the pneumatic three-way valve of the present invention in another state;

FIG. 5 is a partial sectional view of the tray of the present invention;

in the figure, a fixed bracket 1, a collecting channel mounting part 11, a fixed part 12, a protein fraction collecting channel 2, a three-way switching valve 21, a liquid inlet and outlet end 211, a liquid outlet end 212, a pneumatic pump 213, a fraction pipeline 3, a protein storage box 4, a protein collecting bottle 41, a tray 42, a placing surface 421, a placing groove 422, a semiconductor refrigerating sheet 423, a liquid outlet end hose 5 and a liquid channel 6.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

As shown in fig. 1 and fig. 2, the collector dedicated for a protein purification instrument in this embodiment includes a fixing bracket 1 installed on the protein purification instrument, a protein fraction collecting channel 2 is installed outside the fixing bracket 1, the protein fraction collecting channel 2 has a plurality of three-way switching valves 21 which are sequentially and laterally distributed and respectively connected to a control module of the protein purification instrument, liquid inlet and outlet ends 211 of two adjacent three-way switching valves 21 are connected through a fraction pipeline 3, a protein storage tank 4 is installed below the fixing bracket 1, a plurality of protein collecting bottles 41 which are laterally and sequentially distributed and respectively correspond to the three-way switching valves 21 one by one are installed in the protein storage tank 4, and a liquid outlet end 212 of the three-way switching valve 21 is communicated with the protein collecting bottles 41 through a liquid outlet hose 5.

Obviously, after the protein liquid enters the protein fraction collecting channel 2, the control module of the protein purification instrument opens the liquid outlet end 212 of the three-way switching valve 21, and the protein liquid is collected into the protein storage tank 4 for storage, so that the purified protein fractions are effectively collected in batches.

Preferably, the fixing bracket 1 is made of stainless steel plate, the fixing bracket 1 includes a vertically arranged collecting channel installation part 11, the protein fraction collecting channel 2 is installed on the outer surface of the collecting channel installation part 11, the upper end of the collecting channel installation part 11 is provided with an inward bent fixing part 12, the collecting channel installation part 11 and the fixing part 12 are arranged in an L shape, the collecting channel installation part 11 and the fixing part 12 are connected into an integral structure, and the fixing part 12 is fixedly installed on the protein purification instrument through a plurality of screws.

Further, the three-way switching valves 21 are electric three-way valves or pneumatic three-way valves, the number of the three-way switching valves 21 is 12-24, and the three-way switching valves are sequentially and uniformly distributed at equal intervals, for example, as shown in fig. 3-4, when the three-way switching valves 21 are pneumatic three-way valves, the flow direction of the liquid in the pneumatic three-way valves is controlled by the pneumatic pump 213.

As shown in fig. 1, the distillation pipeline 3 is a stainless steel pipe, the distillation pipeline 3 and the three-way switching valve 21 are connected in series in sequence to form a liquid channel 6 transversely penetrating through the fixed bracket 1, and the liquid outlet end hoses 5 are respectively connected with the liquid channel 6. Preferably, the fraction pipes 3 are arranged horizontally and each fraction pipe 3 is arranged coaxially, and the fraction pipes 3 are connected to the collecting channel mounting part 11 by welding or bonding.

Wherein, the liquid outlet end hose 5 is made of rubber material, the liquid outlet end hose 5 is vertically arranged, and the lower end of the liquid outlet end hose 5 is positioned above the protein collecting bottle 41 or the lower end of the liquid outlet end hose 5 extends into the protein collecting bottle 41.

As shown in FIGS. 1 and 5, the protein storage tank 4 is a cryopreservation type tank, and the protein collection bottles 41 are detachably mounted on trays 42 in the protein storage tank 4. The protein storage tank 4 has a low-temperature refrigeration function, can maintain the biological activity of the protein, and further, a large-capacity bottle in the protein storage tank 4 can be replaced to meet the collection requirement.

Wherein, the tray 42 has a placing surface 421 at the upper end, and several placing grooves 422 are provided on the placing surface 421, and the lower end of the protein collecting bottle 41 is placed in the placing grooves 422.

For example, in order to realize the low-temperature refrigeration function of the protein storage box 4, the tray 42 has an inner cavity therein, a plurality of semiconductor refrigeration sheets 423 corresponding to the placing grooves 422 are arranged in the inner cavity of the tray 42, and the refrigeration surfaces of the semiconductor refrigeration sheets 423 are connected with the inner sides of the placing grooves 422 in an adhesion manner.

The specific embodiments described herein are merely illustrative of the spirit of the utility model. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the utility model as defined in the appended claims.

Although terms such as the fixing bracket 1, the collecting channel mounting part 11, the fixing part 12, the protein fraction collecting channel 2, the three-way switching valve 21, the liquid inlet/outlet end 211, the liquid outlet end 212, the pneumatic pump 213, the fraction pipeline 3, the protein storage tank 4, the protein collecting bottle 41, the tray 42, the placing surface 421, the placing groove 422, the semiconductor refrigerating sheet 423, the liquid outlet end hose 5, the liquid channel 6, etc. are used more herein, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed as being without limitation to any additional limitations that may be imposed by the spirit of the present invention.

Claims

1. A special collector for a protein purifier, which comprises a fixed bracket (1) arranged on the protein purifier, it is characterized in that a protein fraction collecting channel (2) is arranged at the outer side of the fixed bracket (1), the protein fraction collecting channel (2) is provided with a plurality of three-way switching valves (21) which are sequentially and transversely distributed and are respectively connected with the control module of the protein purifier, liquid inlet and outlet ends (211) of two adjacent three-way switching valves (21) are connected through a fraction pipeline (3), a protein storage box (4) is arranged below the fixed bracket (1), a plurality of protein collecting bottles (41) which are transversely distributed in sequence and respectively correspond to the three-way switching valves (21) one by one are arranged in the protein storage box (4), and the liquid outlet end (212) of the three-way switching valve (21) is communicated with the protein collecting bottle (41) through a liquid outlet end hose (5).

2. The special collector of a protein purification appearance of claim 1, wherein, fixed bolster (1) include the collection passageway installation department (11) of vertical setting, just protein fraction collection passageway (2) install in collection passageway installation department (11) surface, collection passageway installation department (11) upper end have inside buckling fixed part (12) just collection passageway installation department (11) and fixed part (12) between be the setting of L shape, fixed part (12) through a plurality of screw fixed mounting on protein purification appearance.

3. The special collector of claim 2, wherein the fixing bracket (1) is made of stainless steel plate, and the collecting channel mounting part (11) and the fixing part (12) are connected into an integral structure.

4. The special collector for protein purifiers as claimed in claim 1, 2 or 3, wherein the three-way switching valve (21) is an electric three-way valve or a pneumatic three-way valve, and the three-way switching valves (21) are 12-24 and are sequentially and uniformly distributed at equal intervals.

5. The special collector for the protein purifier as claimed in claim 4, wherein the fraction pipeline (3) is a stainless steel pipe, the fraction pipeline (3) and the three-way switching valve (21) are sequentially connected in series to form a liquid channel (6) transversely penetrating through the fixed support (1), and the liquid outlet end hose (5) is respectively connected with the liquid channel (6).

6. The special collector for the protein purification instrument as claimed in claim 4, wherein the liquid outlet end hose (5) is made of rubber, the liquid outlet end hose (5) is vertically arranged, and the lower end of the liquid outlet end hose (5) is positioned above the protein collection bottle (41) or the lower end of the liquid outlet end hose (5) extends into the protein collection bottle (41).

7. The collector special for protein purifiers according to claim 1, wherein the protein storage tank (4) is a low-temperature storage type storage tank, and the protein collection bottles (41) are sequentially detachably arranged on a tray (42) in the protein storage tank (4).

8. The collector special for protein purifiers according to claim 7, wherein the tray (42) has a placing surface (421) at the upper end, the placing surface (421) is provided with a plurality of placing grooves (422), and the lower end of the protein collection bottle (41) is placed in the placing grooves (422).

9. The collector special for the protein purification instrument as claimed in claim 8, wherein the tray (42) is provided with an inner cavity, a plurality of semiconductor refrigeration pieces (423) corresponding to the placing grooves (422) one by one are arranged in the inner cavity of the tray (42), and the refrigeration surfaces of the semiconductor refrigeration pieces (423) are connected with the inner sides of the placing grooves (422) in a bonding manner.

10. The special collector of a protein purification appearance of claim 2, characterized in that, the fraction pipeline (3) is disposed in horizontal distribution and each fraction pipeline (3) is disposed coaxially, and the fraction pipeline (3) is connected to the collecting channel mounting part (11) by welding or bonding.