CN2169849Y

CN2169849Y - Gravity sample feeder for capillary electrophoretic instrument

Info

Publication number: CN2169849Y
Application number: CN 93211673
Authority: CN
Inventors: 傅小芸; 郑平
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 1993-05-01
Filing date: 1993-05-01
Publication date: 1994-06-22
Anticipated expiration: 2003-05-01

Abstract

The utility model relates to a gravity sample feeder for a capillary electrophoresis instrument, which is composed of a sample bottle 1, an electrolyte solution bottle 2, a quartz capillary tube 8, a vacuum pump 7, connection tubes and electromagnetic valves. The utility model is characterized in that a liquid storage bottle 3 is arranged; the height difference H between the liquid level in the liquid storage bottle and the liquid level in the electrolyte solution bottle is used for gravity sample feeding. The liquid storage bottle and the electrolyte solution bottle are connected together by a tube 9 and the electromagnetic valve 5, and the electrolytic solution bottle is sealed by a cover. The electrolyte solution bottle 2 and the vacuum pump 7 are connected by the tube 10 and the electromagnetic valve 4. The tube 10 is communicated with the atmosphere through the electromagnetic valve 6. The utility model omits a lifting appliance of the sample; the sample feeding precision is high, and the sample feeding control is convenient.

Description

Gravity sample feeder for capillary electrophoretic instrument

The utility model belongs to the sampling device of compartment analysis instrument.

Sampling device is the critical component of capillary electrophoresis apparatus, and its sample size only has several liters of receiving.Existing input mode has gravity, vacuum and electrokinetic injection.The gravity sample introduction claims the siphon sample introduction again.Gravity sample introduction prior art as shown in Figure 1.The mechanical mechanism that this device needs an energy that sample bottle 1 and kapillary 8 are promoted or descend together, sample bottle and kapillary are promoted together, cause the liquid level of sample bottle 1 higher about 10 centimeters than the liquid level of electrolyte solution bottle 2, sample liquid under syphonic effect in the sample bottle 1 enters kapillary, after stopping several seconds sample bottle 1 is descended, make the liquid level of sample bottle 1 and the same height of liquid level of electrolyte solution bottle 2.Control its sample size by changing liquid level difference and retention time.This device needs an aforesaid lifting and decline process, promote, in the decline process also at sample introduction, so influence the precision of sample introduction.And this mechanism's complexity, involve great expense; Kapillary fractures in lifting process easily.

The utility model is that a kind of sampling device will be provided, and its sample bottle and electrolyte solution bottle relative position immobilize, and can save baroque elevating mechanism, avoid kapillary to fracture, and improves the sample introduction precision.

Description of drawings:

Fig. 1 is the structural representation of existing sampling device;

Fig. 2 is the utility model structural representation;

With reference to description of drawings organization plan of the present utility model:

As shown in Figure 2, the utility model is a kind of gravity sampling device of capillary electrophoresis apparatus, it is by sample bottle 1, electrolyte solution bottle 2, quartz capillary 8, vacuum pump 7 and connect pipe and solenoid valve is formed, and its design feature is that a reservoir 3 is arranged, in the reservoir liquid level and

Height difference H is used for the gravity sample introduction between the electrolyte solution bottle 2 interior liquid levels, and electrolyte solution bottle 2 requires sealing, by pipe 9 and solenoid valve 5 reservoir and electrolyte solution bottle is linked together; Electrolyte solution bottle 2 is connected with vacuum pump 7 with solenoid valve 4 by connecting pipe 10, pipe 10 is communicated with atmosphere through solenoid valve 6.

The liquid level of sample bottle 1 and electrolyte solution bottle 2 keeps with high, and the electrolyte solution bottle seals.The liquid level difference H of reservoir 3 liquid levels and electrolyte solution bottle 2 is the siphon height of gravity sample introduction.

The course of work is as follows:

Solenoid valve 4, solenoid valve 6 are opened before the sample introduction, and solenoid valve 5 cuts out, and claim analysis state this moment again.The electrolyte solution bottle leads to atmosphere.

Solenoid valve 4 cuts out during sample introduction, and solenoid valve 5, solenoid valve 6 are opened, and the electrolyte of electrolyte solution bottle 2 enters reservoir 3 by pipe 9 under syphonic effect.Because electrolyte solution bottle 2 is sealed by solenoid valve 4, with atmospheric isolation, electrolyte solution bottle loss electrolyte solution makes the electrolyte solution bottle pressure less than atmospheric pressure, at this moment the sample in the sample bottle 1 enters kapillary 8 under atmospheric effect, capillary inner diameter is about 25～100 μ m, total only several liters of receiving of sample size, sample introduction does not have lifting process, sample introduction precision height.The big liquid level of reservoir diameter can think constant, stops the just enough analyses of several seconds sample size.Finish solenoid valve 4, solenoid valve 6 of sample introduction opened, and solenoid valve 5 cuts out.

During flushing kapillary 8, substitute sample bottle 1 with another electrolyte solution bottle, solenoid valve 5, solenoid valve 6 are closed, and solenoid valve 4 is opened, and opens vacuum pump, and electrolyte solution is pressed into kapillary, and kapillary is washed, and prepares sample introduction next time.

The utility model is simple in structure, and sample introduction control is convenient, sample size precision height, and kapillary is frangibility not, and cost is low.Test shows that the utility model successfully is applied to the sample introduction of capillary electrophoresis apparatus.

Claims

A kind of gravity sampling device of capillary electrophoresis apparatus, comprise sample bottle [1], electrolyte solution bottle [2], quartz capillary [8], vacuum pump [7], connect pipe and solenoid valve, feature of the present utility model is that a reservoir [3] is arranged, height difference H is used for the gravity sample introduction between interior liquid level of reservoir and the interior liquid level of electrolyte solution bottle [2], by pipe [9] and solenoid valve [5] reservoir [3] and electrolyte solution bottle [2] are linked together, require electrolyte solution bottle [2] sealing; Be provided with solenoid valve [4] on the pipe [10] in electrolyte solution bottle [2] and being connected of vacuum pump [7], pipe [10] is communicated with atmosphere through solenoid valve [6].