CN216978766U - Flow cytometer - Google Patents

Abstract

The utility model provides a flow cytometer, which comprises a sheath liquid box body, wherein a liquid supplementing mechanism and 2 sheath liquid barrels with the same structure are arranged in the sheath liquid box body, and the liquid supplementing mechanism is respectively connected with a liquid adding pipe and the sheath liquid barrels; the fluid infusion mechanism comprises an infusion outer tube, an infusion inner tube is arranged inside the infusion outer tube, the infusion inner tube is fixed in the infusion outer tube through a fixing support, and an infusion ball screw is arranged inside the infusion inner tube and connected with the infusion outer tube. According to the flow cytometer, the liquid supplementing mechanism is arranged, so that the sheath liquid in the first sheath liquid barrel can be replaced to the second sheath liquid barrel for liquid taking before the sheath liquid in the first sheath liquid barrel is used up, the first sheath liquid barrel can be taken out at any time for supplementing, the sheath liquid can be supplemented after all experiments are completed, ongoing experiments are not influenced, the experiment efficiency is improved, and the risk of interrupting the experiments is reduced.

Description

Flow cytometer

Technical Field

The utility model belongs to the field of biochemistry, and particularly relates to a flow cytometer.

Background

Flow cytometers are devices that automatically analyze and sort cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to preselected parameter ranges. The selection of cells is accomplished by isolating a stream containing a single cell, which is the sheath fluid. The sheath fluid bucket of current flow cytometer all is fixed size, and the sheath fluid is used up in the experimentation, can only interrupt the experiment, if the data is obtaining, probably causes the loss of data.

Disclosure of Invention

In view of the above, the present invention provides a flow cytometer to overcome the defects in the prior art.

In order to achieve the purpose, the technical scheme of the utility model is realized as follows:

a flow cytometer comprises a sheath fluid box body, wherein a fluid infusion mechanism and 2 sheath fluid buckets with the same structure are arranged inside the sheath fluid box body, and the fluid infusion mechanism is respectively connected with a fluid feeding pipe and the sheath fluid buckets;

fluid infusion mechanism include the fluid infusion outer tube, the inside of fluid infusion outer tube be provided with the fluid infusion inner tube, the fluid infusion inner tube be fixed in through the fixed bolster the fluid infusion outer tube in, the inside of fluid infusion inner tube is provided with fluid infusion ball and links to each other, fluid infusion ball and rotating electrical machines link to each other, the nut of fluid infusion ball on be provided with the sepage board, the fluid infusion inner tube on be equipped with liquid outlet and 2 inlets, the liquid outlet with the liquid feeding pipe link to each other, the inlet link to each other with the sheath liquid bucket that corresponds, the inlet be located respectively the both sides of sepage board.

Further, the outer diameter of the liquid separation plate is matched with the inner diameter of the liquid supplementing inner pipe. The middle lines of the liquid separation plate and the liquid supplementing inner tube are mutually vertical, and sheath liquid on two sides can be separated, so that the purpose of replacing the sheath liquid barrel is realized.

Further, a pipette is arranged inside the sheath liquid barrel, a barrel cover is arranged at the top of the sheath liquid barrel, and the top of the pipette penetrates through the barrel cover and is fixed on the barrel cover through a fixing plug.

Further, a liquid suction connecting piece is arranged at the top of the liquid suction pipe, one end of the liquid suction connecting piece is connected with the liquid suction pipe, and the other end of the liquid suction connecting piece is detachably connected with the liquid inlet. The imbibition connecting piece adopts a conventional structure, and is convenient to detach and connect.

Further, the top of sheath liquid box be equipped with the filling opening, the tip of filling tube be provided with the liquid feeding connecting piece, the liquid feeding connecting piece pass the filling opening with the liquid outlet can dismantle the connection. The liquid feeding connecting piece adopts a conventional structure, and is convenient to detach and connect.

Further, the bottom of the sheath liquid barrel is provided with a liquid level sensor. The liquid level sensor is connected with a controller of the flow cytometer through a line, and can transmit signals to the controller when the liquid level is too low, and the controller can control the rotating motor to drive the liquid separation plate to move so as to replace the sheath liquid barrel.

Compared with the prior art, the utility model has the following advantages:

according to the flow cytometer, the liquid supplementing mechanism is arranged, so that the sheath liquid in the first sheath liquid barrel can be replaced to the second sheath liquid barrel for liquid taking before the sheath liquid in the first sheath liquid barrel is used up, the first sheath liquid barrel can be taken out at any time for supplementing, the sheath liquid can be supplemented after experiments are completed completely, ongoing experiments are not influenced, the experiment efficiency is improved, and the risk of interrupting the experiments is reduced.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate an embodiment of the utility model and, together with the description, serve to explain the utility model and not to limit the utility model. In the drawings:

FIG. 1 is a schematic view of a flow cytometer in accordance with an embodiment of the present invention;

fig. 2 is a schematic view of a fluid infusion mechanism according to an embodiment of the present invention.

Description of reference numerals:

1. a sheath fluid tank; 2. a sheath fluid barrel; 3. a barrel cover; 4. a pipette; 5. a fixed plug; 6. a liquid suction connector; 7. a liquid supplementing outer pipe; 8. a fixed mount; 9. a liquid filling port; 10. a liquid feeding connector; 11. a liquid feeding pipe; 12. a liquid level sensor; 71. a liquid supplementing inner pipe; 72. fixing a bracket; 73. a liquid supplementing ball screw; 74. a rotating electric machine; 75. a liquid barrier; 76. a liquid inlet; 77. and a liquid outlet.

Detailed Description

It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

As shown in fig. 1-2, a flow cytometer includes a sheath fluid tank 1, wherein a fluid infusion mechanism and 2 sheath fluid barrels 2 having the same structure are disposed inside the sheath fluid tank 1, and the fluid infusion mechanism is respectively connected to a fluid feeding tube 11 and the sheath fluid barrels 2; fluid infusion mechanism include fluid infusion outer tube 7, the inside of fluid infusion outer tube 7 be provided with fluid infusion inner tube 71, fluid infusion inner tube 71 be fixed in through fixed bolster 72 fluid infusion outer tube 7 in, the inside of fluid infusion inner tube 71 is provided with fluid infusion ball screw 73 and links to each other, fluid infusion ball screw 73 and rotating electrical machines 74 link to each other, fluid infusion ball screw 73's nut on be provided with liquid partition 75, fluid infusion inner tube 71 on be equipped with liquid outlet 77 and 2 inlet 76, liquid outlet 77 with filling tube 11 link to each other, inlet 76 and the sheath liquid bucket 2 that correspond link to each other, inlet 76 be located respectively liquid partition 75's both sides. The outer diameter of the liquid separation plate 75 is matched with the inner diameter of the liquid supplementing inner pipe 71. The liquid separation plate 75 is perpendicular to the center line of the inner fluid infusion tube 71, so that sheath fluid on two sides can be separated, and the purpose of replacing the sheath fluid barrel 2 is achieved.

A pipette 4 is arranged in the sheath liquid barrel 2, a barrel cover 3 is arranged at the top of the sheath liquid barrel 2, and the top of the pipette 4 penetrates through the barrel cover 3 and is fixed on the barrel cover 3 through a fixing plug 5. The top of the pipette 4 is provided with a pipette connector 6, one end of the pipette connector 6 is connected with the pipette 4, and the other end is detachably connected with the liquid inlet 76. The liquid suction connecting piece 6 adopts a conventional structure, and is convenient to detach and connect. Sheath liquid box 1's top be equipped with filling opening 9, the tip of filling tube 11 be provided with liquid feeding connecting piece 10, liquid feeding connecting piece 10 pass filling opening 9 with liquid outlet 77 can dismantle the connection. The liquid feeding connecting piece 10 adopts a conventional structure, and is convenient to detach and connect.

And liquid level sensors 12 are arranged at the bottoms of the sheath liquid barrels 2. The liquid level sensor 12 is connected with a controller of the flow cytometer through a line, and can transmit signals to the controller when the liquid level is too low, and the controller can control the rotating motor 74 to drive the liquid separation plate 75 to move, so as to replace the sheath liquid barrel 2. The level sensor 12, the rotary motor 74 and the controller are all commercially available products.

The implementation process comprises the following steps:

before the experiment, the liquid adding pipe 11 is connected with the liquid adding connecting piece 10, the liquid adding connecting piece 10 is connected with the liquid outlet 77 of the liquid supplementing inner pipe 71, then the liquid suction pipes 4 of 2 sheath liquid barrels 2 are respectively connected with the corresponding liquid suction connecting pieces 6, and the liquid suction connecting pieces 6 are connected with the corresponding liquid inlets 76.

In the experiment, the sheath liquid in the first sheath liquid barrel 2 is used, at this time, the liquid isolating plate 75 is located between the liquid outlet 77 and the second liquid inlet 76 corresponding to the second sheath liquid barrel 2, the liquid isolating plate 75 blocks the passage to the second liquid inlet 76, when the sheath liquid in the first sheath liquid barrel 2 is about to be used up, the liquid level sensor 12 in the first sheath liquid barrel 2 sends a signal, the controller starts the rotating motor 74 to drive the liquid supplementing ball screw 73 to rotate, the liquid isolating plate 75 is driven to move to the first liquid inlet 76 corresponding to the first sheath liquid barrel 2 until the liquid isolating plate 75 moves to the position between the first liquid inlet 76 and the liquid outlet 77, the passage of the first liquid inlet 76 is blocked, and then the sheath liquid barrel 2 is replaced.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.

Claims (6)

1. A flow cytometer, comprising: the sheath liquid filling device comprises a sheath liquid box body, wherein a liquid supplementing mechanism and 2 sheath liquid barrels with the same structure are arranged in the sheath liquid box body, and the liquid supplementing mechanism is respectively connected with a liquid adding pipe and the sheath liquid barrels;

fluid infusion mechanism include the fluid infusion outer tube, the inside of fluid infusion outer tube be provided with the fluid infusion inner tube, the fluid infusion inner tube be fixed in through the fixed bolster the fluid infusion outer tube in, the inside of fluid infusion inner tube is provided with fluid infusion ball, fluid infusion ball link to each other with rotating electrical machines, fluid infusion ball's nut on be provided with the liquid trap, the fluid infusion inner tube on be equipped with liquid outlet and 2 inlets, the liquid outlet with the liquid feeding pipe link to each other, inlet and the sheath liquid bucket that corresponds link to each other, the inlet be located respectively the both sides of liquid trap.

2. A flow cytometer as described in claim 1 wherein: the outer diameter of the liquid separation plate is matched with the inner diameter of the liquid supplementing inner pipe.

3. A flow cytometer as described in claim 1 wherein: the sheath liquid barrel is characterized in that a liquid suction pipe is arranged inside the sheath liquid barrel, a barrel cover is arranged at the top of the sheath liquid barrel, and the top of the liquid suction pipe penetrates through the barrel cover and is fixed on the barrel cover through a fixing plug.

4. A flow cytometer as described in claim 3 wherein: the top of the liquid suction pipe is provided with a liquid suction connecting piece, one end of the liquid suction connecting piece is connected with the liquid suction pipe, and the other end of the liquid suction connecting piece is detachably connected with the liquid inlet.

5. A flow cytometer as described in claim 1 wherein: the top of sheath liquid box be equipped with the filling opening, the tip of filling tube be provided with the liquid feeding connecting piece, the liquid feeding connecting piece pass the filling opening with the liquid outlet can dismantle the connection.

6. A flow cytometer as described in claim 1 wherein: and liquid level sensors are arranged at the bottoms of the sheath liquid barrels.

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