CN216972503U - Kit for extracting nucleic acid - Google Patents

Abstract

The utility model provides a kit for extracting nucleic acid, and relates to the field of diagnostic extraction reagents. The kit comprises: the box body is provided with a hollow accommodating cavity; the arrangement frames are provided with the sinking grooves and the through holes, and the arrangement frames are arranged in the accommodating cavity along the depth direction of the through holes; the reagent plate is at least partially positioned in the sink groove; and a plurality of parts of the magnetic rod sleeve are respectively accommodated in the through holes of the arraying frames along the length direction of the magnetic rod. The reagent kit has the advantages that the reagent plate and the magnetic rod sleeve containing the magnetic rod are reasonably arranged in the reagent kit, and the space occupied by the reagent kit is reduced.

Description

Kit for extracting nucleic acid

Technical Field

The utility model relates to the field of diagnostic extraction reagents, in particular to a kit for extracting nucleic acid.

Background

Before carrying out in vitro detection of nucleic acid reagents, the nucleic acid needs to be extracted. The nucleic acid is extracted by a magnetic bead method, namely, the nucleic acid molecules can be specifically identified and combined with silicon hydroxyl on the surface of the magnetic bead, and are aggregated or dispersed under the action of an external magnetic field, so that the nucleic acid is extracted.

The related kit for extracting nucleic acid by using the magnetic bead method comprises a reagent plate and a magnetic rod, but the reagent plate and the magnetic rod are placed in a messy manner in the box body, so that the kit needs to occupy a larger space.

SUMMERY OF THE UTILITY MODEL

The utility model provides a kit for extracting nucleic acid, which is used for solving the technical problem of how to reasonably arrange a reagent plate and a magnetic rod in a box body and reduce the occupied space.

The embodiment of the utility model provides a kit for extracting nucleic acid, which comprises: the box body is provided with a hollow accommodating cavity; the arrangement frames are provided with sinking grooves and through holes, and the arrangement frames are arranged in the accommodating cavity along the depth direction of the through holes; a reagent plate at least partially positioned within the sink; and a plurality of parts of the magnetic rod sleeve are respectively accommodated in the through holes of the arraying racks along the length direction of the magnetic rod.

Further, the arrangement racks are arranged along the vertical direction, and the top surfaces of the arrangement racks can be attached to the bottom surfaces of the adjacent arrangement racks.

Further, the dimension of the sink groove in the depth direction is not less than the thickness dimension of the reagent plate.

Further, the sink includes: the first subslot is formed by recessing the top surface of the arrangement frame; the second subslot is formed by inwards recessing the bottom surfaces of the arrangement racks; wherein the first subslot and the second subslot are arranged at intervals, and the sum of the depth dimension of the first subslot and the depth of the second subslot is larger than the thickness dimension of the reagent plate; the depth dimension of the first subslot is smaller than the thickness dimension of the reagent plate.

Furthermore, the top surface of the arraying frame is provided with a first positioning structure, the bottom surface of the arraying frame is provided with a second positioning structure, the first positioning structure and the second positioning structure of the adjacent arraying frame are abutted, and the first sub-groove and the second sub-groove of the adjacent arraying frame can be communicated.

Further, the kit further comprises: an end frame provided with a first end frame groove; wherein, in the extending direction of through-hole, the end frame is located the top the alignment frame, just first end frame groove can with first subslot intercommunication, and/or, the end frame is located the bottom the alignment frame's below, just first end frame groove can with second subslot intercommunication.

Further, the depth dimension of the first end frame groove is smaller than the thickness dimension of the end frame.

Furthermore, the end frame is also provided with a second end frame groove which can be communicated with the through hole; in the extending direction of the through hole, the sum of the depth dimension of the second end frame groove and the depth dimension of the through hole of each of the alignment frames is larger than the height dimension of the auxiliary member.

Furthermore, a plurality of the sinking grooves are arranged on the arrangement frame at intervals.

Further, the kit also comprises a filler positioned between the arrangement rack and the inner wall of the containing cavity.

The embodiment of the utility model provides a kit for extracting nucleic acid, which comprises a box body with a hollow accommodating cavity, a plurality of arrangement racks with a sink groove and through holes, a reagent plate at least partially positioned in the sink groove, and a magnetic rod sleeve accommodating a magnetic rod, wherein a plurality of parts of the magnetic rod sleeve are respectively accommodated in the through holes of the arrangement racks along the length direction of the magnetic rod. The arrangement frame is arranged in the containing cavity along the depth direction of the through holes, namely, the arrangement frame is arranged in the containing cavity, so that the direction of the maximum size of the reagent plate and the direction of the maximum size of the magnetic rod are arranged in the containing cavity in a non-parallel mode, the space in the containing cavity is utilized more fully, the volume required by the containing cavity is reduced, and the occupied space of the reagent kit is reduced. Simultaneously, through set up the alignment frame holding the intracavity, can hold the intracavity with reagent board and the bar magnet cover interval that holds the bar magnet to reduce the reagent board and the possibility that the collision between the bar magnet cover that holds the bar magnet and damage, improved the reliability that the kit extracted nucleic acid.

Drawings

FIG. 1 is a schematic diagram of a nucleic acid extraction kit according to an embodiment of the present invention;

FIG. 2 is a schematic view showing an assembly of an alignment rack, a reagent plate and auxiliary members in the kit for extracting nucleic acid according to the embodiment of the present invention;

FIG. 3 is a schematic assembly view of a reagent plate, an auxiliary member, and a first type of rack in the kit for extracting nucleic acid according to the embodiment of the present invention;

FIG. 4 is a schematic assembly view of a reagent plate, an auxiliary member, and a second type of rack in the kit for extracting nucleic acid according to the embodiment of the present invention;

FIG. 5 is a schematic view showing the assembly of a reagent plate, auxiliary members, alignment shelves and end shelves in the kit for extracting nucleic acid according to the embodiment of the present invention;

FIG. 6 is a schematic diagram showing the structure of an arrangement rack in a nucleic acid extraction kit according to an embodiment of the present invention;

FIG. 7 is a schematic structural diagram of another nucleic acid extraction kit according to an embodiment of the present invention.

Description of the reference numerals

1. A kit; 10. a box body; 11. an accommodating chamber; 20. an arrangement rack; 20A, a first type of alignment rack; 20B, a second type of alignment rack; 21. sinking a groove; 211. a first subslot; 212. a second subslot; 22. a through hole; 23. a first positioning structure; 24. a second positioning structure; 30. a reagent plate; 40. a magnetic rod sleeve; 50. an end frame; 51. a first end frame slot; 52. a second end frame slot; 60. and (4) filling materials.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

The individual features described in the embodiments can be combined in any suitable manner without departing from the scope, for example different embodiments and aspects can be formed by combining different features. In order to avoid unnecessary repetition, various possible combinations of the specific features of the utility model will not be described further.

In the following description, the term "first/second/so" is used merely to distinguish different objects and does not mean that there is a common or relationship between the objects. It should be understood that the description of the "upper", "lower", "outer" and "inner" directions as related to the orientation in the normal use state, and the "left" and "right" directions indicate the left and right directions indicated in the corresponding schematic drawings, and may or may not be the left and right directions in the normal use state.

It should be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element. The term "coupled", where not otherwise specified, includes both direct and indirect connections.

In the following embodiments, the kit for extracting nucleic acid may be used for extracting nucleic acid by a magnetic bead method, and the extracted nucleic acid may be used for in vitro disease detection.

In some embodiments, as shown in fig. 1, kit 1 comprises: the reagent kit comprises a box body 10, an arrangement frame 20, a reagent plate 30 and a magnetic rod sleeve 40 containing magnetic rods. The box body 10 has a hollow accommodating cavity 11 therein, and the accommodating cavity 11 has at least one opening through which the arrangement frame 20, the reagent plate 30 and the magnetic rod sleeve 40 can be put into or taken out of the accommodating cavity 11. The arrangement rack 20 is provided with a plurality of grooves 21 and through holes 22, each arrangement rack 20 is arranged in the accommodating cavity 11 along the depth direction of the through hole 22, illustratively, the depth direction of the through hole 22 extends along the vertical direction, and each arrangement rack 20 is arranged along the vertical direction; illustratively, the depth direction of the through holes 22 extends in the vertical direction, and the respective alignment racks 20 are aligned in the vertical direction. For convenience of description, the structure of the reagent cartridge 1 will be exemplified below by taking the through-holes 22 extending in the vertical direction and the arrangement of the respective racks 20 in the vertical direction in the accommodation chamber 11 as an example.

At least a part of the reagent plate 30 is located in the sinking groove 21, it can be understood that the reagent plate 30 is accommodated in the sinking groove 21, the lateral movement of the reagent plate 30 in the accommodating chamber 11 is limited by the side wall of the sinking groove 21, meanwhile, the reagent plate 30 is located between the two alignment racks 20 in the vertical direction, and the alignment rack 20 located above can abut against the top surface of the reagent plate 30 to limit the movement of the reagent plate 30 in the vertical direction in the accommodating chamber 11. The plurality of parts of the magnetic rod sleeve 40 are respectively accommodated in the through holes 22 of the respective arrangement racks 20 along the length direction of the magnetic rod, and in particular, the magnetic rod sleeve 40 passes through the through holes 22 of the plurality of arrangement racks 20 along the length direction of the magnetic rod, thereby restricting the movement of the magnetic rod sleeve 40 in the accommodation chamber 11 by the through holes of the plurality of arrangement racks 20. Wherein, the positions of the through holes 22 of the plurality of shelves 20 correspond to the shape of the magnet bar sleeve 40, so that the magnet bar sleeve 40 can pass through the through holes 22 of the plurality of shelves 20, illustratively, the maximum dimension direction of the magnet bar sleeve 40 extends along a circular arc curve, the through holes 22 of the plurality of shelves 20 are arranged at intervals along the circular arc curve, so that the magnet bar sleeve 40 can pass through the through holes 22 of each shelf 20 along the magnet bar sleeve 40; illustratively, the maximum dimension direction of the magnet bar sleeve 40 extends along a straight line, the plurality of arrangement racks 20 are arranged along a vertical direction, and the through holes 22 of the respective arrangement racks 20 are arranged along the vertical direction, so that the magnet bar sleeve 40 can penetrate through the through holes 22 of the respective arrangement racks 20 along the vertical direction.

It should be noted that, the size of the magnetic rod in the length direction is much larger than the maximum size of the reagent plate 30, that is, the direction of the maximum size of the magnetic rod sleeve 40 is much larger than the maximum size of the reagent plate 30, if the direction of the maximum size of the reagent plate 30 and the direction of the maximum size of the magnetic rod sleeve 40 are arranged in parallel in the accommodating cavity, a large amount of space in the accommodating cavity 11 is wasted, which results in a need to provide the accommodating cavity 11 with a large volume, and meanwhile, since it is difficult for the reagent plate 30 and the magnetic rod sleeve 40 to completely fill the accommodating cavity 11, a gap exists between the reagent plate 30 and the magnetic rod sleeve 40, and a collision may occur between the reagent plate 30 and the magnetic rod sleeve 40 during transportation. Through setting up a plurality of ranks 20, each rank 20 all is provided with heavy groove 21 and through-hole 22 and each rank 20 arranges along through-hole 22's distribution direction, a plurality of reagent boards 30 at least part holds respectively in the heavy groove 21 of each rank 20, reagent board 30 runs through the through-hole 22 of a plurality of ranks 20, so that nonparallel arrangement between the direction of the maximum size of reagent board 30 and the maximum size of bar magnet cover 40, thereby more abundant utilization has been carried out to the space that holds chamber 11, required volume that holds chamber 11 has been reduced, and then the shared space of kit 1 has been reduced. Meanwhile, the reagent plate 30 and the magnetic rod sleeve 40 are separated in the accommodating cavity 11 by arranging the arraying frame 20, so that the possibility of damage caused by collision between the reagent plate 30 and the magnetic rod sleeve 40 is reduced, and the reliability of the reagent kit 1 is improved.

The embodiment of the utility model provides a kit, which comprises a box body with a hollow accommodating cavity, a plurality of arrangement racks provided with a sinking groove and through holes, a reagent plate at least partially positioned in the sinking groove, and a plurality of magnetic rod sleeves partially accommodated in the through holes respectively. The arrangement frame is arranged in the containing cavity along the depth direction of the through holes, namely, the arrangement frame is arranged in the containing cavity, so that the direction of the maximum size of the reagent plate and the direction of the maximum size of the magnetic rod sleeve are arranged in the containing cavity in a non-parallel mode, the space in the containing cavity is utilized more fully, the volume required by the containing cavity is reduced, and the occupied space of the kit is further reduced. Simultaneously, through set up the alignment frame holding the intracavity, can hold intracavity with reagent board and bar magnet cover interval to reduce the collision between reagent board and the bar magnet cover and the possibility of damaging, improved the reliability of kit.

In some embodiments, as shown in fig. 2, each rack 20 is arranged in a vertical direction (the vertical direction is indicated by an arrow in fig. 2), and a top surface of each rack 20 can be attached to a bottom surface of an adjacent rack 20, and it is understood that, in a state where the reagent plate 30 is accommodated in the sink 21 of the reagent plate 30, the bottom surface of the rack 20 located above the rack 20 does not interfere with the reagent plate 30 in the sink 21, so that the bottom surface of the rack 20 located above the rack 20 is attached to the top surface of the rack 20, and thus, by a space surrounded by an inner wall of the sink of the rack 20 and the bottom surface located above the rack 20, the vertical movement of the reagent plate 30 and the vertical movement of the reagent plate 30 are restricted, the movement of the reagent plate 30 is restricted more reliably, and, at the same time, the bottom surface of the rack 20 can contact the top surface located below the rack 20, so that the rack 20 can also be engaged with the top surface located below the rack 20 The aligning frame 20 below the aligning frame 20 supports the aligning frame 20 more reliably. It should be noted that the structure of the alignment rack 20 may be any structure that can make the bottom surface of the alignment rack 20 fit with the top surface of the alignment rack 20 located below the alignment rack 20, and the structure of the alignment rack 20 will be exemplarily described below with reference to fig. 3 and 4.

As shown in fig. 3, the dimension of the sink 21 in the depth direction of the first type rack 20A is not smaller than the thickness dimension of the reagent plate 30, so that the top surface of the reagent plate 30 is flush with the top surface of the first type rack 20A or the top surface of the reagent plate 30 is lower than the top surface of the first type rack 20A in a state where the reagent plate 30 is accommodated in the sink 21, whereby the bottom surface of the first type rack 20A located above the first type rack 20A does not interfere with the reagent plate 30 in the sink 21. Optionally, the arrangement rack 20A of the first type is further provided with an avoiding groove adjacent to the sinking groove 21 for increasing a gap between an inner wall of the sinking groove 21 and the reagent plate 30, thereby facilitating the placement of the reagent plate 30 into the sinking groove 21 or the removal of the reagent plate 30 from the sinking groove 21.

As shown in fig. 4, the sinking groove 21 of the second type arrangement rack 20B includes a first sub-groove 211 and a second sub-groove 212, the first sub-groove 211 is formed by an inner concavity of a top surface of the second type arrangement rack 20B, and the second sub-groove 212 is formed by an inner concavity of a bottom surface of the second type arrangement rack 20B, wherein the first sub-groove 211 and the second sub-groove 212 are spaced apart, i.e., a predetermined distance is provided between a bottom surface of the first sub-groove 211 and a bottom surface of the second sub-groove 212. The sum of the depth dimension of the first subslot 211 and the depth dimension of the second subslot 212 is greater than the thickness dimension of the reagent plate 30, and the depth dimension of the first subslot 211 is less than the thickness dimension of the reagent plate 30, in a state where the reagent plate 30 is put into the first sub-well 211 of one of the second type of arrangement racks 20B, another second type of arrangement rack 20B is placed above the second type of arrangement rack 20B from above, so that the reagent plate 30 is located in the receiving space surrounded by the inner walls of the first subslot 211 and the second subslot 212 of two adjacent second type arrangement racks 20B, so that the movement of the reagent plate 30 relative to the second type of rack 20B is limited by the first and second sub-wells 211 and 212 of two adjacent second type of rack 20B, and the bottom surface of the second type arrangement rack 20B located above can be made to fit with the top surface of the second type arrangement rack 20B located below. Meanwhile, the depth dimension of the first sub-groove 211 is smaller than the thickness dimension of the reagent plate 30 so that the reagent plate 30 can protrude from the first sub-groove 211, and thus, after the second type of rack 20B located above is removed, the reagent plate 30 can be easily taken out from the first sub-groove 211 of the second type of rack 20B located below.

Optionally, the top surface of the second type arrangement rack 20B is further provided with a first positioning structure 23, the bottom surface of the second type arrangement rack 20B is further provided with a second positioning structure 24, the first positioning structure 23 of the adjacent second type arrangement rack 20B is abutted to the second positioning structure 24, so that the acting force between the first positioning structure 23 and the second positioning structure 24 can realize the positioning between the adjacent second type arrangement racks 20B, and the first sub-groove 211 and the second sub-groove 212 of the adjacent second type arrangement rack 20B are communicated, so that the first sub-groove 211 and the second sub-groove 212 can form an accommodating space for accommodating the reagent plate 30. The first positioning structure 23 and the second positioning structure 24 are any structures capable of positioning two adjacent second-type arrangement racks 20B, for example, the first positioning structure 23 may be a positioning pin, the second positioning structure 24 may be a positioning hole matched with the positioning pin, and in a state that the positioning pin is inserted into the positioning hole, the first sub-groove 211 and the second sub-groove 212 of the two adjacent second-type arrangement racks 20B are communicated; for example, the first positioning structure 23 may be a positioning groove, and the second positioning structure 24 may be a positioning boss cooperating with the positioning groove, and in a state where the positioning boss is located in the positioning groove, the first sub-groove 211 and the second sub-groove 212 of two adjacent second type arrangement racks 20B communicate.

In some embodiments, as shown in fig. 5, the reagent cartridge 1 further includes an end frame 50, and the end frame 50 is provided with a first end frame slot 51, wherein the end frame 50 is located above the array frame 20 at the top of each array frame 20 and/or the end frame 50 is located below the array frame 20 at the bottom of each array frame 20 in the extending direction of the through holes 22 of the array frame 20, for convenience of description, the end frame 50 located below the array frame 20 at the bottom of each array frame 20 is referred to as a bottom plate, the end frame 50 located above the array frame 20 at the top of each array frame 20 is referred to as a top plate, and the arrangement frame 20 can be isolated from the bottom surface or the top surface of the accommodating cavity 11 by providing the bottom plate and the top plate, so as to further improve the protection effect of the reagent plate 30 and the magnetic rod cover 40 accommodated in the array frame 20. In a state where the arrangement rack 20 is the second type of arrangement rack 20B, the first end rack groove 51 of the top cover can communicate with the first sub-groove 211 to form an accommodation space for accommodating the reagent plate 30 together with the first sub-groove 211, and the first end rack groove 51 of the bottom plate can communicate with the second sub-groove 212 to form an accommodation space for accommodating the reagent plate 30 together with the second sub-groove 212. Alternatively, the depth dimension of the first end frame groove 51 is smaller than the thickness dimension of the end frame 50, and it is understood that the first end frame groove 51 does not penetrate both end surfaces of the end frame 50 in the direction of the width dimension of the end frame 50, so that the bottom surface of the first end frame groove 51 can cover the first sub-groove 211 and the second sub-groove 212, thereby protecting the reagent plate 30 accommodated in the first end frame groove 51 more reliably.

In some embodiments, as shown in fig. 5, the end frame 50 is further provided with a second end frame slot 52 capable of communicating with the through hole 22 in fig. 1, and in the extending direction of the through hole 22, the sum of the depth dimension of the second end frame slot 52 and the depth dimension of the through hole 22 of each row frame 20 is greater than the height dimension of the bar sleeve 40, so that the second end frame slot 52 and the through hole 22 of each row frame 20 jointly form a receiving space for receiving the bar sleeve 40, and the bar sleeve 40 does not protrude from the receiving space above the receiving space. Alternatively, the second end frame slot 52 may have a dimension in the depth direction smaller than the thickness dimension of the end frame 50, that is, the second end frame slot 52 may not extend through both end surfaces of the end frame 50 in the direction of the thickness dimension of the end frame 50, so that the bottom surface of the second end frame slot 52 can cover the through hole 22, thereby more reliably protecting the portion of the magnet bar cover 40 received in the second end frame slot 52.

In some embodiments, as shown in fig. 6, the arrangement rack 20 is provided with a plurality of sinks 21 at intervals to accommodate a plurality of reagent plates 30 by using the dimension of the arrangement rack 20 perpendicular to the extending direction of the through holes 22, so that the dimension of the arrangement rack 20 perpendicular to the extending direction of the through holes 22 is more fully utilized, the volume of the accommodating cavity 11 required in fig. 1 is further reduced, and the occupied space of the reagent kit 1 is reduced.

In some embodiments, as shown in fig. 7, the reagent kit further comprises a filler 60, and the filler 60 is located between the alignment rack 20 and the inner wall of the accommodating cavity 11 in fig. 1, and is used for filling a gap between the alignment rack 20 and the inner wall of the accommodating cavity 11, so as to limit the movement of the alignment rack 20 relative to the accommodating cavity 11 in fig. 1, and thus, to more reliably protect the reagent plate 30 and the magnetic rod sleeve 40 accommodated in the alignment rack 20.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention.

Claims (10)

1. A kit for extracting nucleic acid, the kit comprising:

the box body is provided with a hollow accommodating cavity;

the arrangement frames are provided with sinking grooves and through holes, and are arranged in the accommodating cavity along the depth direction of the through holes;

a reagent plate at least partially positioned within the sink;

and a plurality of parts of the magnetic rod sleeve are respectively accommodated in the through holes of the arraying racks along the length direction of the magnetic rod.

2. The kit according to claim 1, wherein the shelves are arranged in a vertical direction, and top surfaces of the shelves are capable of fitting against bottom surfaces of adjacent shelves.

3. The reagent cartridge according to claim 2, wherein a dimension of the sink in a depth direction is not smaller than a thickness dimension of the reagent plate.

4. The kit of claim 2, wherein the sink comprises:

the first subslot is formed by recessing the top surface of the arrangement frame;

the second subslot is formed by inwards recessing the bottom surfaces of the arrangement racks;

wherein the first subslot and the second subslot are arranged at intervals, and the sum of the depth dimension of the first subslot and the depth of the second subslot is larger than the thickness dimension of the reagent plate; the depth dimension of the first subslot is smaller than the thickness dimension of the reagent plate.

5. The reagent cartridge according to claim 4, wherein the top surface of the alignment rack is provided with a first positioning structure, the bottom surface of the alignment rack is provided with a second positioning structure, and the first positioning structure and the second positioning structure of the adjacent alignment rack are abutted to enable the first sub-groove and the second sub-groove of the adjacent alignment rack to communicate with each other.

6. The kit of claim 4, further comprising:

an end frame provided with a first end frame groove;

wherein, in the extending direction of the through hole, the end frame is positioned above the arrangement frame at the top, and the first end frame groove can be communicated with the first sub-groove, and/or,

the end frame is located at the bottom and below the arrangement frame, and the first end frame groove can be communicated with the second sub-groove.

7. The kit of claim 6, wherein the first end frame slot has a depth dimension that is less than a thickness dimension of the end frame.

8. The kit of claim 6, wherein the end frame is further provided with a second end frame slot capable of communicating with the through hole; in the extending direction of the through hole, the sum of the depth dimension of the second end frame groove and the depth dimension of the through hole of each alignment frame is larger than the height dimension of the magnetic rod sleeve.

9. The kit according to claim 2, wherein the rack is provided with a plurality of the sinks at intervals.

10. The kit of claim 1, further comprising a filler between the alignment rack and the inner wall of the receiving cavity.

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