CN216919252U - Detection apparatus for nonshou classical swine fever virus nucleic acid in tick worm - Google Patents
Detection apparatus for nonshou classical swine fever virus nucleic acid in tick worm Download PDFInfo
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- CN216919252U CN216919252U CN202220572489.XU CN202220572489U CN216919252U CN 216919252 U CN216919252 U CN 216919252U CN 202220572489 U CN202220572489 U CN 202220572489U CN 216919252 U CN216919252 U CN 216919252U
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- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 21
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 21
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 18
- 241000710777 Classical swine fever virus Species 0.000 title claims description 8
- 241000701386 African swine fever virus Species 0.000 claims abstract description 9
- 241000238876 Acari Species 0.000 claims description 15
- 238000007599 discharging Methods 0.000 claims description 7
- 239000000523 sample Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 2
- LXWYCLOUQZZDBD-LIYNQYRNSA-N csfv Chemical compound C([C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)[C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O)C1=CC=C(O)C=C1 LXWYCLOUQZZDBD-LIYNQYRNSA-N 0.000 claims 4
- 241000282898 Sus scrofa Species 0.000 abstract description 11
- 238000009434 installation Methods 0.000 description 4
- -1 CSFV nucleic acid Chemical class 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 210000003127 knee Anatomy 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/84—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with two or more stirrers rotating at different speeds or in opposite directions about the same axis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/201—Holders therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/25—Mixing the contents of independent containers, e.g. test tubes the containers being submitted to a combination of movements other than within a horizontal plane, e.g. rectilinear and pivoting movement
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the technical field of detection equipment, and particularly relates to a detection device for nucleic acid of African swine fever virus in tick, aiming at the problems that a scattering mechanism for detecting the nucleic acid of the African swine fever virus in tick is single in scattering mode, the scattering speed is low, and the detection efficiency is influenced. According to the utility model, the first scattering rod and the second scattering rod are driven to rotate in opposite directions, and the detection box is driven to shake left and right and up and down, so that tissues in the pig body are scattered efficiently, and the efficiency and accuracy of detection are improved.
Description
Technical Field
The utility model relates to the technical field of detection equipment, in particular to a detection device for nucleic acid of African swine fever virus in ticks.
Background
Ticks are widely distributed in various regions of the world, and appropriate host blood suction needs to be found at each growth stage. The most serious hazard of ticks is that the various pathogens carried by their bodies can spread widely during blood sucking and host replacement. Ticks transmit a variety of pathogens, including viruses, bacteria, protozoa, etc., and African swine fever virus has been shown to be one of these, and the transmission pathway of the virus includes transmission via biological vectors, such as ticks. Therefore, the rapid and accurate diagnosis of whether the tick is carrying and transmitting the African swine fever virus is an important step for controlling and preventing the infectious disease.
When the African swine fever virus is detected, tissues in a pig body are required to be taken out, then the tissues are scattered and quickly detected, the existing scattering mechanism is single in scattering mode, the scattering speed is low, and the detection efficiency is affected, so that the detection device for the nucleic acid of the African swine fever virus in ticks is provided for solving the problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the defects that the existing scattering mechanism for detecting the nucleic acid of the nonsense swine fever virus in the tick is single in scattering mode, low in scattering speed and low in detection efficiency, and provides a device for detecting the nucleic acid of the nonsense swine fever virus in the tick.
In order to achieve the purpose, the utility model adopts the following technical scheme:
the utility model provides a detection apparatus for nonsense swine fever virus nucleic acid in tick worm, includes base and detection case, fixed mounting has test probe on the left side inner wall of detection case, and the top of detection case has seted up the round hole, and the drum is installed to the round hole internal rotation, and the vertical axis is installed to the internal rotation of drum, and the outside fixed mounting of vertical axis has the first pole of breaing up, and the outside of vertical axis is fixed to be cup jointed first bevel gear, the equal fixed mounting in both sides of drum has the knee, and the equal fixed mounting in one side that two knee are close to each other has a plurality of seconds to breaing up the pole, and the outside fixed hub of drum has the second bevel gear, the top fixed mounting of detection case has first motor, fixedly connected with third bevel gear on the output shaft of first motor, and first bevel gear and second bevel gear all mesh with the third bevel gear, the slide opening has been seted up at the top of base, and slidable mounting has the slide in the slide opening, the top fixed mounting of slide has the connecting plate, and the equal fixed mounting in top four corners of connecting plate has the montant, the equal fixed mounting in both sides of detection case has two diaphragms, and four diaphragms slide respectively and cup joint in the outside of the montant that corresponds, the equal fixed mounting in both sides has two fixed axles around the detection case, and the runner has been cup jointed in the outside rotation of fixed axle, the equal fixed mounting in both sides has two arcs around the top of base, four runners respectively with the top activity butt of the arc that corresponds.
Preferably, the front side bottom fixed mounting of slide has a circle axle, and the outside of circle axle is rotated and has been cup jointed the commentaries on classics board, base bottom fixed mounting has the mounting panel, and the bottom fixed mounting of mounting panel has the second motor, fixedly connected with rotor arm on the output shaft of second motor, and the front side bottom fixed mounting of rotor arm has the round bar, changes the board and rotates and cup joint in the outside of round bar.
Preferably, the top fixed mounting of montant has the baffle, the bottom fixedly connected with reset spring of baffle, and four reset spring's bottom difference fixed connection are at the top of the diaphragm that corresponds.
Preferably, the inner walls of the two sides of the sliding hole are fixedly connected with the same guide rod, and the sliding plate is sleeved on the outer side of the guide rod in a sliding mode.
Preferably, the equal fixed mounting in both sides has two installation axles around the connecting plate, and the outside of installation axle is rotated and is cup jointed the supporting wheel, the top roll connection of supporting wheel and base.
Preferably, the top intercommunication of detection case has the inlet pipe, and the right side bottom intercommunication of detection case has the discharging pipe, and fixed mounting has the control valve on the discharging pipe.
According to the detection device for the nucleic acid of the African swine fever virus in ticks, tissues in a pig body are added into a detection box through a feeding pipe, a first motor and a second motor are started, an output shaft of the first motor drives a third bevel gear to rotate, a vertical shaft and a cylinder are driven to rotate oppositely through meshing of the first bevel gear and a second bevel gear, the vertical shaft drives a first scattering rod to rotate forwards, the cylinder drives a second scattering rod to rotate reversely through a bent rod, and therefore the tissues in the pig body are scattered;
according to the device for detecting the nucleic acid of the nonsense swine fever virus in the tick pests, the second motor is operated and simultaneously drives the rotating arm to rotate, the rotating arm drives the sliding plate to reciprocate left and right through the cooperation of the round rod and the rotating plate, the sliding plate drives the connecting plate to reciprocate left and right, the connecting plate drives the detection box to reciprocate left and right, the arc-shaped plate drives the detection box to move up and down through abutting against the rotating wheel, so that tissues in a pig body in the detection box can shake left and right and up and down, the scattering efficiency is improved, the components of the scattered components can be frequently detected through the detection probe and are discharged through the discharge pipe;
the pig tissue scattering device is reasonable in structural design, the first scattering rod and the second scattering rod are driven to rotate in opposite directions, and the detection box is driven to swing left and right and up and down, so that tissues in a pig body are scattered efficiently, the efficiency and accuracy of detection of the tissues are improved, and the pig tissue scattering device is high in reliability.
Drawings
FIG. 1 is a schematic perspective view of a device for detecting CSFV nucleic acid in ticks according to the present invention;
FIG. 2 is an enlarged view of a portion A of FIG. 1;
FIG. 3 is a schematic cross-sectional view of a device for detecting CSFV nucleic acid in ticks according to the present invention;
FIG. 4 is a schematic perspective view of another embodiment of the apparatus for detecting CSFV nucleic acid in ticks according to the present invention.
In the figure: 1. a base; 2. a detection box; 3. a connecting plate; 4. a first motor; 5. a second motor; 6. a first bevel gear; 7. a second bevel gear; 8. a third bevel gear; 9. a cylinder; 10. detecting a probe; 11. a first scatter bar; 12. a second breaker bar; 13. a vertical axis; 14. a slide plate; 15. rotating the plate; 16. a rotating arm; 17. a transverse plate; 18. a return spring; 19. a rotating wheel; 20. an arc-shaped plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-4, a device for detecting nucleic acid of nonsense swine fever virus in tick pests, which comprises a base 1 and a detection box 2, wherein a detection probe 10 is fixedly installed on the inner wall of the left side of the detection box 2, a round hole is formed in the top of the detection box 2, a cylinder 9 is rotatably installed in the round hole, a vertical shaft 13 is rotatably installed in the cylinder 9, a first scattering rod 11 is fixedly installed on the outer side of the vertical shaft 13, a first bevel gear 6 is fixedly sleeved on the outer side of the vertical shaft 13, bent rods are fixedly installed on both sides of the cylinder 9, a plurality of second scattering rods 12 are fixedly installed on the mutually close sides of the two bent rods, a second bevel gear 7 is fixedly sleeved on the outer side of the cylinder 9, a first motor 4 is fixedly installed on the top of the detection box 2, a third bevel gear 8 is fixedly connected on the output shaft of the first motor 4, the first bevel gear 6 and the second bevel gear 7 are both meshed with the third bevel gear 8, the slide opening has been seted up at base 1's top, slidable mounting has slide 14 in the slide opening, slide 14's top fixed mounting has connecting plate 3, the equal fixed mounting in top four corners of connecting plate 3 has the montant, the equal fixed mounting in both sides of detection case 2 has two diaphragms 17, four diaphragms 17 slide respectively and cup joint in the outside of the montant that corresponds, the equal fixed mounting in both sides has two fixed axles around the detection case 2, runner 19 has been cup jointed in the outside rotation of fixed axle, the equal fixed mounting in both sides has two arcs 20 around base 1's the top, four runner 19 respectively with the top activity butt of the arc 20 that corresponds.
According to the utility model, a round shaft is fixedly arranged at the bottom of the front side of the sliding plate 14, a rotating plate 15 is rotatably sleeved on the outer side of the round shaft, an installation plate is fixedly arranged at the bottom of the base 1, a second motor 5 is fixedly arranged at the bottom of the installation plate, a rotating arm 16 is fixedly connected to an output shaft of the second motor 5, a round rod is fixedly arranged at the bottom of the front side of the rotating arm 16, and the rotating plate 15 is rotatably sleeved on the outer side of the round rod, so that the sliding plate 14 can be driven to move left and right.
In the utility model, the top end of the vertical rod is fixedly provided with a baffle, the bottom of the baffle is fixedly connected with the return springs 18, and the bottom ends of the four return springs 18 are respectively and fixedly connected with the top of the corresponding transverse plate 17 to reset the transverse plate 17.
In the utility model, the same guide rod is fixedly connected to the inner walls of the two sides of the sliding hole, and the sliding plate 14 is slidably sleeved on the outer side of the guide rod to guide the sliding plate 14.
According to the utility model, two mounting shafts are fixedly mounted on the front side and the rear side of the connecting plate 3, supporting wheels are rotatably sleeved on the outer sides of the mounting shafts, and the supporting wheels are in rolling connection with the top of the base 1 to support the connecting plate 3.
According to the utility model, the top of the detection box 2 is communicated with a feeding pipe, the bottom of the right side of the detection box 2 is communicated with a discharging pipe, and a control valve is fixedly arranged on the discharging pipe, so that the detection box 2 can be conveniently fed and discharged.
In the utility model, when in use, tissues in a pig body are added into a detection box 2 through a feeding pipe, a first motor 4 and a second motor 5 are started, an output shaft of the first motor 4 drives a third bevel gear 8 to rotate, a vertical shaft 13 and a cylinder 9 are driven to rotate oppositely through meshing of a first bevel gear 6 and a second bevel gear 7, the vertical shaft 13 drives a first scattering rod 11 to rotate forwards, the cylinder 9 drives a second scattering rod 12 to rotate reversely through a bent rod, so that the tissues in the pig body are scattered, the second motor 5 drives a rotating arm 16 to rotate while rotating, the rotating arm 16 drives a sliding plate 14 to reciprocate left and right through matching of a round rod and the rotating plate 15, the sliding plate 14 drives a connecting plate 3 to reciprocate left and right, the connecting plate 3 drives the detection box 2 to reciprocate left and right, the arc-shaped plate 20 drives the detection box 2 to move left and right and up and down through abutting of a rotating wheel 19, thereby make the internal tissue of pig in detection case 2 control and rock from top to bottom, promote and break up efficiency, break up back accessible test probe 10 and often detect its composition to discharge through the discharging pipe.
Claims (6)
1. The detection device for nucleic acid of African swine fever virus in tick comprises a base (1) and a detection box (2), and is characterized in that a detection probe (10) is fixedly mounted on the inner wall of the left side of the detection box (2), a round hole is formed in the top of the detection box (2), a cylinder (9) is mounted in the round hole in a rotating manner, a vertical shaft (13) is mounted in the rotating manner of the cylinder (9), a first scattering rod (11) is fixedly mounted on the outer side of the vertical shaft (13), a first bevel gear (6) is fixedly sleeved on the outer side of the vertical shaft (13), bent rods are fixedly mounted on both sides of the cylinder (9), a plurality of second scattering rods (12) are fixedly mounted on one side, close to each other, of the two bent rods, a second bevel gear (7) is fixedly sleeved on the outer side of the cylinder (9), a first motor (4) is fixedly mounted on the top of the detection box (2), a third bevel gear (8) is fixedly connected on the output shaft of the first motor (4), first bevel gear (6) and second bevel gear (7) all mesh with third bevel gear (8) mutually, the slide opening has been seted up at the top of base (1), and slidable mounting has slide (14) in the slide opening, and the top fixed mounting of slide (14) has connecting plate (3), and the equal fixed mounting in top four corners of connecting plate (3) has the montant, the equal fixed mounting in both sides of detection case (2) has two diaphragms (17), and four diaphragms (17) slide respectively and cup joint in the outside of the montant that corresponds, the equal fixed mounting in both sides has two fixed axles around detection case (2), and runner (19) have been cup jointed in the outside rotation of fixed axle, the equal fixed mounting in both sides has two arcs (20) around the top of base (1), and four runners (19) respectively with the top activity butt of the arc (20) that correspond.
2. The device for detecting the nucleic acid of the CSFV in ticks, according to claim 1, characterized in that a round shaft is fixedly mounted at the bottom of the front side of the sliding plate (14), a rotating plate (15) is rotatably sleeved at the outer side of the round shaft, a mounting plate is fixedly mounted at the bottom of the base (1), a second motor (5) is fixedly mounted at the bottom of the mounting plate, a rotating arm (16) is fixedly connected to the output shaft of the second motor (5), a round rod is fixedly mounted at the bottom of the front side of the rotating arm (16), and the rotating plate (15) is rotatably sleeved at the outer side of the round rod.
3. The device for detecting the nucleic acid of the CSFV in ticks, according to the claim 1, is characterized in that the top end of the vertical rod is fixedly provided with a baffle, the bottom of the baffle is fixedly connected with a return spring (18), and the bottom ends of the four return springs (18) are respectively and fixedly connected with the top of the corresponding transverse plate (17).
4. The device for detecting the nucleic acid of the CSFV in ticks, according to the claim 1, is characterized in that the inner walls of the two sides of the slide hole are fixedly connected with the same guide rod, and the slide plate (14) is sleeved outside the guide rod in a sliding way.
5. The device for detecting the nucleic acid of the CSFV in ticks, according to the claim 1, is characterized in that two mounting shafts are fixedly mounted on the front and the rear sides of the connecting plate (3), the outer side of the mounting shafts is rotatably sleeved with a supporting wheel, and the supporting wheel is connected with the top of the base (1) in a rolling manner.
6. The device for detecting the nucleic acid of the hog cholera virus in the ticks, as per claim 1, wherein a feeding pipe is communicated with the top of the detection box (2), a discharging pipe is communicated with the bottom of the right side of the detection box (2), and a control valve is fixedly mounted on the discharging pipe.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220572489.XU CN216919252U (en) | 2022-03-16 | 2022-03-16 | Detection apparatus for nonshou classical swine fever virus nucleic acid in tick worm |
| DE202022101882.0U DE202022101882U1 (en) | 2022-03-16 | 2022-04-07 | African swine fever virus nucleic acid detection device in ticks |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220572489.XU CN216919252U (en) | 2022-03-16 | 2022-03-16 | Detection apparatus for nonshou classical swine fever virus nucleic acid in tick worm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216919252U true CN216919252U (en) | 2022-07-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220572489.XU Active CN216919252U (en) | 2022-03-16 | 2022-03-16 | Detection apparatus for nonshou classical swine fever virus nucleic acid in tick worm |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN216919252U (en) |
| DE (1) | DE202022101882U1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116179324B (en) * | 2022-12-30 | 2024-04-26 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Poultry virus detection device and detection method |
-
2022
- 2022-03-16 CN CN202220572489.XU patent/CN216919252U/en active Active
- 2022-04-07 DE DE202022101882.0U patent/DE202022101882U1/en active Active
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| Publication number | Publication date |
|---|---|
| DE202022101882U1 (en) | 2022-06-07 |
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Effective date of registration: 20240126 Address after: Room 101, Building 14, Liandong U-Valley Industrial Park, No. 869 Huaguan Road, High tech Zone, Qingdao City, Shandong Province, 266000 Patentee after: Adiwei (Qingdao) Biotechnology Co.,Ltd. Country or region after: China Address before: 250023 No. 202, Gongye North Road, Jinan, Shandong Province Patentee before: Poultry Research Institute of Shandong Academy of Agricultural Sciences Country or region before: China Patentee before: INSTITUTE OF ANIMAL SCIENCE AND VETERINARY MEDICINE SHANDONG ACADEMY OF AGRICULTURAL SCIENCES |