CN216919249U

CN216919249U - Cattle genome DNA keeps device

Info

Publication number: CN216919249U
Application number: CN202220478450.1U
Authority: CN
Inventors: 赵小东
Original assignee: Ordos Saibaoyin Ecological Animal Husbandry Co ltd
Current assignee: Ordos Saibaoyin Ecological Animal Husbandry Co ltd
Priority date: 2022-03-07
Filing date: 2022-03-07
Publication date: 2022-07-08
Anticipated expiration: 2032-03-07

Abstract

The application provides a kind of cattle genome DNA keeps the device, includes: test tubes, cotton swabs, membranes and sealing structures; the test tube is used for placing the genomic DNA preserving fluid; a thin film parallel to the liquid surface is arranged at the upper end of the liquid surface of the genomic DNA preservation solution, the edge of the thin film is hermetically connected with the side wall of the test tube, and the thin film is used for sealing and preserving the genomic DNA preservation solution; the cotton swab comprises a swab rod and a cotton ball, wherein one end of the swab rod is conical, the cotton ball is arranged close to the conical position, and the cotton ball is adhered with a genome DNA (deoxyribonucleic acid) obtained in advance; when one end of the swab rod enters the test tube, the cotton swab can puncture the film through the conical structure, so that the cotton ball enters the genomic DNA preservation solution to realize genomic DNA preservation; the sealing structure is used for sealing the top of the test tube after the preservation of the genome DNA is realized. According to the embodiment of the application, the embodiment of the application not only improves the sealing effect of the film, but also reduces the pollution to genome DNA.

Description

Cattle genome DNA keeps device

Technical Field

The application relates to the technical field of animal husbandry, especially, relate to a breeding cattle genome DNA keeps device.

Background

In the field of animal husbandry, it is often necessary to preserve the basic group of DNA of livestock, for example the bovine genomic DNA.

Current genomic DNA preservation devices include test tubes, cotton swabs and membranes. Wherein, place genome DNA preserving fluid in the test tube, be stained with genome DNA on the cotton swab, the film sets up in genome DNA preserving fluid upper end for the preserving genome DNA preserving fluid of sealing. When the kit is used, the film is punctured by the cotton swab, and the cotton ball of the cotton swab is placed into the genomic DNA preservation solution.

As is clear from the above, the conventional technique has a problem that when a film is punctured with a cotton swab, the film is cracked, or genomic DNA is adhered to the film, which results in a decrease in the sealing effect of the film or contamination of the genomic DNA.

SUMMERY OF THE UTILITY MODEL

The application provides a kind of cattle genome DNA keeps device for solve the problem that the easy crack that produces when puncturing the film among the prior art reduces the sealed effect of film.

In a first aspect, the present application provides a bovine genomic DNA storage apparatus comprising: test tubes, cotton swabs, membranes and sealing structures;

the test tube is used for placing genome DNA preserving fluid;

a thin film parallel to the liquid surface is arranged at the upper end of the liquid surface of the genomic DNA preservation solution, the edge of the thin film is hermetically connected with the side wall of the test tube, and the thin film is used for sealing and preserving the genomic DNA preservation solution;

the cotton swab comprises a swab rod and a cotton ball, wherein one end of the swab rod is conical, the cotton ball is arranged close to the conical position, and the cotton ball is adhered with a genome DNA (deoxyribonucleic acid) obtained in advance;

when one end of the swab rod enters the test tube, the cotton swab can puncture the film through the conical structure, so that the cotton ball enters the genomic DNA preservation solution to realize genomic DNA preservation;

the sealing structure is used for sealing the top of the test tube after the preservation of the genome DNA is realized.

Optionally, the bovine genomic DNA storage device further comprises a test tube cover corresponding to the test tube;

the test tube cover is detachably connected with the tube opening of the test tube;

the other end fixed connection who wipes the pole is on the inside top of test tube cap, and when the test tube cap entered into the inside default position of test tube mouth, the film can be punctured to the cotton swab to make the cotton ball enter into in the genomic DNA conservator solution.

Optionally, the test tube lid can be dismantled with the mouth of pipe department of test tube and be connected including: the upper end of test tube is provided with first internal thread, and the test tube lid is provided with the first external screw thread that corresponds with first internal thread, and threaded connection is realized through first internal thread and first external screw thread to test tube lid and test tube.

Optionally, the sealing structure is a first sealing surface for sealing the upper end of the test tube cap and the test tube port after the preservation of the genomic DNA is realized.

Optionally, a first inner annular surface is arranged at the bottom end of the film along the side wall of the test tube, and the first inner annular surface is used for adhering the film.

Optionally, the orificial size of test tube and the other end size of wiping the pole are corresponding, and mouth of pipe department is provided with the second internal thread, and the other end of wiping the pole is provided with the outer screw thread of second, and threaded connection is realized through second internal thread and the outer screw thread of second to the test tube and cotton swab.

Optionally, the bovine genomic DNA storage device further comprises a second sealing surface for sealing the test tube port and the other end of the swab rod after genomic DNA storage is achieved.

Optionally, a second inner annular surface is arranged at the bottom end of the film along the side wall of the test tube, and the second inner annular surface is used for adhering the film.

Optionally, a shockproof structure is arranged outside the test tube and used for buffering external force applied to the test tube.

Optionally, the maximum diameter of the cotton ball is smaller than the diameter of the tube orifice of the test tube.

From the above, the cattle genome DNA preservation device provided by the embodiment of the application comprises a test tube, a film, a sealing structure and a cotton swab. Wherein, the one end of swabbing the pole that cotton swab included is the toper form, when stabbing the film through the toper form like this, compare in prior art and directly use the cotton ball to stab more easily, can reduce moreover and produce the crack on stabbing the in-process film, not only make the film seal up the effect better, stab the contact that the film can reduce cotton ball and film through the toper form moreover, consequently can also reduce the pollution of film to ox genome DNA.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural diagram of a bovine genomic DNA storage apparatus according to an embodiment of the present disclosure;

FIG. 2 is a schematic view of a cotton ball according to yet another embodiment of the present disclosure before entering into a genomic DNA preservation solution;

FIG. 3 is a schematic view of a cotton ball according to another embodiment of the present disclosure after entering into a genomic DNA preservation solution;

FIG. 4 is a schematic structural view of a bovine genomic DNA storage apparatus according to still another embodiment of the present invention;

FIG. 5 is a schematic structural view of a bovine genomic DNA storage apparatus according to still another embodiment of the present application;

FIG. 6 is a schematic view of a cotton ball before entering into a genomic DNA preservation solution according to yet another embodiment of the present application;

FIG. 7 is a diagram illustrating a state of a cotton ball after entering into a genomic DNA preservation solution according to still another embodiment of the present application;

FIG. 8 is a schematic structural diagram of a bovine genomic DNA storage apparatus according to another embodiment of the present application;

FIG. 9 is a schematic structural diagram of a bovine genomic DNA storage apparatus according to still another embodiment of the present application;

FIG. 10 is a schematic structural diagram of a bovine genomic DNA storage apparatus according to still another embodiment of the present application.

In the figure: the reagent kit comprises a shockproof structure 1, a test tube 100, a first internal thread 101, a genomic DNA preservative solution 102, a thin film 200, a sealing structure 300, a first sealing surface 301, a second sealing surface 302, a cotton swab 400, a swab 401, a cotton ball 402, a second external thread 403, a test tube cap 500, a first external thread 501, an internal ring surface 600, a first internal ring surface 601, a second internal ring surface 602, an internal ring platform 700 and a second internal thread 701.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application are clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present application, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

FIG. 1 shows a device for preserving bovine genomic DNA according to the present embodiment. As shown in fig. 1, the bovine genomic DNA storage apparatus includes: a test tube 100, a membrane 200, a sealing structure 300, and a cotton swab 400.

The test tube 100 is used for placing a genomic DNA preservative solution 102.

A thin film 200 parallel to the liquid surface is provided at the upper end of the liquid surface of the genomic DNA preservative solution 102, the edge of the thin film 200 is hermetically connected to the side wall of the test tube 100, and the thin film 200 is used for sealing the genomic DNA preservative solution 102.

The cotton swab 400 comprises a swab 401 and a cotton ball 402, wherein one end of the swab 401 is conical, the cotton ball 402 is arranged close to the conical, and the cotton ball 402 is adhered with a genome DNA obtained in advance.

When one end of the swab 401 enters the inside of the test tube 100, the cotton swab 400 may puncture the film 200 through the conical structure, so that the cotton ball 402 enters the genomic DNA preservation solution 102 to preserve the genomic DNA.

The sealing structure 300 is used to seal the top of the test tube 100 after the preservation of genomic DNA is achieved.

The bovine genomic DNA preservation device has the following use process:

first, a cotton swab 400 is used to obtain the genomic DNA of a cow to be stored, and the obtained genomic DNA to be stored is adhered to a cotton ball 402. The cotton swab 400 is then inserted into the test tube 100, with the end of the swab 401 approaching and piercing the membrane 200 through the conical structure, until the cotton ball 402 enters the genomic DNA preservative solution 102. Finally, the top of the test tube 100 is sealed using the sealing structure 300 to achieve a sealing effect.

From the above, the bovine genomic DNA storage apparatus provided in the embodiment of the present application includes the test tube 100, the film 200, the sealing structure 300, and the cotton swab 400. Wherein, the one end of the swab rod 401 that the cotton swab 400 includes is the toper form, like this when puncturing the membrane 200 through the toper form, compare and directly use cotton ball 402 to puncture more easily in prior art, can reduce moreover and produce the fracture on puncturing in-process membrane 200 for membrane 200 seals up the effect better.

In addition, since the conical piercing of the film 200 can reduce the contact between the cotton ball 402 and the film 200, the contamination of the film 200 with the bovine genomic DNA can be reduced.

In addition, since the conical piercing of the film 200 can reduce the contact between the cotton ball 402 and the film 200, the problem that the bovine genomic DNA on the cotton ball 402 is adhered to the film 200 can be reduced, and thus the loss of the obtained bovine genomic DNA can be minimized.

Alternatively, the insertion of the cotton swab 400 into the test tube 100 may be performed manually or with the aid of a tool.

Alternatively, referring to fig. 2 and 3, the bovine genomic DNA storage apparatus further includes a tube cap 500 corresponding to the tube 100.

The test tube cover 500 is detachably connected to the tube opening of the test tube 100.

The other end of the swab 401 is fixedly connected to the top end of the inside of the test tube cap 500, and when the test tube cap 500 enters a predetermined position inside the test tube port of the test tube 100, the cotton swab 400 can puncture the film 200, so that the cotton ball 402 enters the genomic DNA preservative solution 102.

Alternatively, still referring to fig. 2 and 3, the detachable connection of the tube cap 500 to the tube 100 at the nozzle comprises: the upper end of test tube 100 is provided with first internal thread 101, and test tube lid 500 is provided with the first external screw thread 501 that corresponds with first internal thread 101, and test tube lid 500 realizes threaded connection through first internal thread 101 and first external screw thread 501 with test tube 100.

Alternatively, a first groove having a specific shape may be provided on the upper surface of the cap 500, and when it is necessary to screw the cap 500 into the test tube 100, the first groove may be formed by an external force tool.

Illustratively, the first groove is a cross-shaped groove, so that a cross screwdriver can be used to match the cross-shaped groove to screw the test tube cap 500 into the test tube 100, and the labor-saving effect is achieved.

Note that fig. 2 is a state diagram before the cotton ball 402 enters the genomic DNA storage solution 102, and fig. 3 is a state diagram after the cotton ball 402 enters the genomic DNA storage solution 102.

Alternatively, referring to fig. 4, the sealing structure 300 is a first sealing surface 301, and the first sealing surface 301 is used for sealing the upper end of the tube cover 500 and the opening of the test tube 100 after the preservation of the genomic DNA is achieved.

Alternatively, the first sealing surface 301 may be a soft sealing surface, so that when the upper end of the test tube cover 500 is not aligned with the test tube port of the test tube 100, the sealing effect can still be achieved.

Optionally, referring to fig. 5 and 6, an inner annular surface 600 is disposed along the sidewall of the cuvette 100 at the bottom end of the membrane 200, and the inner annular surface 600 is used for adhering the membrane 200.

After the bottom end of the film 200 is provided with the inner ring surface 600 along the side wall of the test tube 100, the film 200 is adhered through the inner ring surface 600, and compared with the case that the film 200 is only connected through the side wall of the test tube 100, the film 200 can be adhered more firmly.

Further, referring to fig. 5, a first inner annular surface 601 is disposed at the bottom end of the film 200 along the sidewall of the test tube 100, and the first inner annular surface 601 is used for adhering the film 200.

Optionally, the size of the nozzle of the test tube 100 corresponds to the size of the other end of the swab 401, a second internal thread 701 is provided at the nozzle, a second external thread 403 is provided at the other end of the swab 401, and the test tube 100 and the cotton swab 400 are in threaded connection through the second internal thread 701 and the second external thread 403.

Further, referring to fig. 6 to 9, the size of the nozzle of the test tube 100 corresponding to the size of the other end of the swab 401 may be determined by providing an inner ring stage 700 at the nozzle of the test tube 100, and the second internal thread 701 is provided inside the inner ring stage 700.

In use, the swab 401 is rotated to allow the swab 401 to enter the test tube 100 and reach the film 200, and the film 200 is punctured to allow the cotton ball 402 to enter the genomic DNA preservative solution 102.

Alternatively, the end surface of the top of one end of the swab 401 is provided with a second groove of a specific shape, which is accessible by means of an external force tool when the swab 401 is screwed into the test tube 100.

The second groove is exemplarily a cross-shaped groove, so that a cross screwdriver can be used to match the cross-shaped groove, so as to screw the swab bar 401 into the test tube 100, and achieve a labor-saving effect.

Note that fig. 6 is a state diagram before the cotton ball 402 enters the genomic DNA storage solution 102, and fig. 7 is a state diagram after the cotton ball 402 enters the genomic DNA storage solution 102.

Further, referring to fig. 8, the bovine genomic DNA storage apparatus further comprises a second sealing surface 302, wherein the second sealing surface 302 is used for sealing the test tube port and the other end of the swab 401 after the preservation of the genomic DNA is achieved.

Alternatively, the second sealing surface 302 may be a soft sealing surface so that when the ends of the test tube port and the other end of the swab 401 are not in a straight line, a sealing effect is achieved.

Further, referring to fig. 9, a second inner annular surface 602 is disposed along the sidewall of the test tube at the bottom end of the membrane 200, and the second inner annular surface 602 is used for adhering the membrane 200.

Alternatively, referring to fig. 10, a shock-proof structure 1 is disposed outside the test tube 100, and the shock-proof structure 1 is used for buffering external force applied to the test tube 100.

When external force striking test tube 100, test tube 100 drops or directly is broken very easily, through shockproof structure 1 buffering test tube 100 received external force after, can reduce external force to test tube 100's extrusion or clash to reduce test tube 100 and drop or broken possibility, improve test tube 100's security.

Optionally, the maximum diameter of cotton ball 402 is less than the diameter of the orifice of test tube 100.

When the maximum diameter of the cotton ball 402 is smaller than the diameter of the nozzle of the test tube 100, the cotton ball 402 can enter the genomic DNA preservative solution 102 without contacting the test tube 100.

Therefore, the pollution of the test tube 100 to the bovine genome DNA can be reduced; the probability that the cattle genome DNA on the cotton ball 402 is adhered to the test tube 100 can be reduced, and therefore, the loss of the obtained cattle genome DNA can be reduced to the greatest extent.

Finally, it should be noted that all the contents not described in the technical solutions of the present application can be implemented by using the prior art. In addition, the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art; the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A kind of cattle genome DNA keeps the device, characterized by that, the said cattle genome DNA keeps the device and includes: test tubes, cotton swabs, membranes and sealing structures;

the test tube is used for placing a genomic DNA preservation solution;

a thin film parallel to the liquid level is arranged at the upper end of the liquid level of the genomic DNA preservation solution, the edge of the thin film is hermetically connected with the side wall of the test tube, and the thin film is used for sealing the genomic DNA preservation solution;

the cotton swab comprises a swab rod and a cotton ball, wherein one end of the swab rod is conical, the cotton ball is arranged close to the conical position, and the cotton ball is adhered with a genome DNA obtained in advance;

when one end of the swab rod enters the inside of the test tube, the cotton swab can puncture the thin film through the conical structure, so that the cotton ball enters the genomic DNA preservation solution to preserve the genomic DNA;

the sealing structure is used for sealing the top of the test tube after the genomic DNA is preserved.

2. The bovine genomic DNA storage apparatus according to claim 1, further comprising a test tube cover corresponding to the test tube;

the other end of the swab rod is fixedly connected to the top end inside the test tube cover, and when the test tube cover enters a preset position inside the test tube opening, the cotton swab can puncture the film, so that the cotton ball enters the genomic DNA preservation solution.

3. The bovine genomic DNA storage apparatus of claim 2, wherein the detachable connection of the tube cover to the tube orifice comprises: the upper end of test tube is provided with first internal thread, the test tube lid be provided with the first external screw thread that first internal thread corresponds, the test tube lid with the test tube passes through first internal thread with threaded connection is realized to first external screw thread.

4. The bovine genomic DNA storage device according to claim 3, wherein the sealing structure is a first sealing surface for sealing the upper end of the test tube cap and the test tube port after the preservation of the genomic DNA is realized.

5. The bovine genomic DNA storage device according to claim 4, wherein a first inner annular surface is arranged at the bottom end of the membrane along the side wall of the test tube, and the first inner annular surface is used for adhering the membrane.

6. The bovine genomic DNA preservation apparatus according to claim 1, wherein the tube has a nozzle corresponding to the other end of the swab, a second internal thread is provided at the nozzle, a second external thread is provided at the other end of the swab, and the tube and the cotton swab are screwed together by the second internal thread and the second external thread.

7. The apparatus for preserving bovine genomic DNA according to claim 6, further comprising a second sealing surface for sealing the test tube port and the other end of the swab shaft after the preservation of the genomic DNA is achieved.

8. The bovine genomic DNA preservation apparatus according to claim 7, wherein a second internal toroid is disposed along the side wall of the test tube at the bottom end of the membrane, and the second internal toroid is used for adhering the membrane.

9. The apparatus for preserving bovine genomic DNA according to any one of claims 1 to 8, wherein a shock-proof structure is provided outside the test tube, and the shock-proof structure is configured to buffer an external force applied to the test tube.

10. The bovine genomic DNA storage device according to claim 9, wherein the cotton ball has a maximum diameter smaller than the diameter of the tube orifice.