CN216899848U - Tissue decalcification instrument - Google Patents

Abstract

The utility model provides a tissue decalcification apparatus, comprising: the kit comprises at least one layer of reagent rack, a plurality of containers and a plurality of water flushing solutions, wherein the containers are placed on the at least one layer of reagent rack and used for containing the decalcification solution, the stationary solution and the water flushing solution; the placing basket is used for placing an embedding box, and tissues to be processed are stored in the embedding box; the arm is placed the basket and is installed in the arm, and the arm can be put into the container with the embedding box to decalcify the tissue. The utility model solves the technical problems of complicated process and easy error of the conventional manual decalcification process of pathological tissues, realizes simple operation process and easy use, can simultaneously treat tissues with different decalcification requirements, has standardized tissue treatment and good repeatability, and has the technical effects of quality assurance on later-stage dyeing, immunization and molecular detection of the decalcification of the tissues.

Description

Tissue decalcification instrument

Technical Field

The utility model relates to the technical field of pathological analysis, in particular to a tissue decalcification instrument.

Background

When a pathology analyst diagnoses bone diseases and diseases related to bone changes, a pathological section examination is often required to be performed on a bone specimen of a diseased part so as to determine diagnosis or provide a basis for diagnosis; after the bone tissue of the lesion site is surgically removed, pathological section examination is also performed, and a bone specimen for pathological examination must be decalcified to be sectioned and observed under a microscope. A large amount of inorganic salt and calcium salt exist in bone tissues, ossified and calcified tissues, bone marrow puncture and bone tumor specimens, the calcium salt is removed by acid firstly when the conventional slicing is made, the conventional slicing can be carried out, and decalcification in the slicing of the bone tissues is an important link and is also a technical difficulty.

The existing decalcification of pathological tissues is carried out manually, and the problems are as follows: the process is complicated and easy to make mistakes, excessive tissue decalcification is easily caused, and histochemical staining, immunohistochemical staining and molecular sequencing analysis cannot be carried out on the tissue, so that the pathological morphological diagnosis is difficult, and the timely treatment of patients is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model solves the technical problems of complicated process and easy error of the conventional manual decalcification process of pathological tissues, provides the tissue decalcification instrument, realizes simple operation process and easy use, can simultaneously or randomly process the decalcification, water washing and fixing requirements of various tissues with different decalcification requirements, has the technical effects of standardized tissue treatment and good repeatability, and has quality assurance on later-stage dyeing, immunization and molecular detection of the tissue decalcification.

In order to solve the above problems, the present invention provides a tissue decalcifying apparatus, comprising: the kit comprises at least one layer of reagent rack, a plurality of containers and a plurality of water flushing solutions, wherein the containers are placed on the at least one layer of reagent rack and used for containing the decalcification solution, the stationary solution and the water flushing solution; the placing basket is used for placing an embedding box, and tissues to be processed are stored in the embedding box; the arm is placed the basket and is installed in the arm, and the arm can be put into the container with the embedding box to decalcify the tissue.

Compared with the prior art, the technical scheme has the following technical effects: pathological bone tissue, bone marrow tissue or severely calcified tissue can be processed by decalcification treatment to be processed by conventional flaking, staining, immunological marking and molecular detection. The decalcification reagent, the decalcification time, the washing time, the fixing liquid and the fixing time which are required by different bone tissues with different components and different degrees of ossification are different, so that the factors such as personnel, treatment conditions and the like are not fixed during daily treatment, unstable factors are caused to the tissue decalcification treatment, the problems of excessive decalcification, wrong decalcification fixing reagent and the like are often caused easily, the later-stage dyeing, the immunological typing and the molecular detection cannot be carried out, and the diagnosis and the treatment are seriously influenced. At present, almost no ideal decalcification device special for pathological tissues exists, and the requirements of decalcification conditions of multiple tissues simultaneously or in multiple batches and different conditions of washing and fixing different tissues simultaneously cannot be met. Therefore, the utility model provides a tissue decalcification instrument, which is provided with a plurality of containers for containing liquid for decalcification and fixation, wherein the placing basket is placed into the containers by the mechanical arm, and the embedding box can be immersed by the liquid in the containers, so that the tissue is decalcification, fixed and washed by water, time and labor are saved, and manpower is not required. In addition, the tissue decalcification instrument provided by the utility model can simultaneously carry out decalcification, washing and fixing requirements of various tissues under different conditions, has simple and easy-to-use operation process, standardized tissue treatment and good repeatability, and has quality assurance on later-stage staining, immunization and molecular detection of the tissue decalcification.

In one example of the present invention, a placement basket comprises: place basket lifting frame, place basket lifting frame's both ends and be provided with the hand-basket and detain, place basket lifting frame and detain through the hand-basket and install in the arm.

Compared with the prior art, the technical scheme has the following technical effects: the arm passes the hand-basket and detains, and then places the basket and can hang on the arm, places the basket and follows the arm motion, places the basket and simple and convenient, the easy operation of installation in arm department.

In one example of the utility model, the robotic arm is provided with a hook that passes through the basket buckle so that the placement basket hangs from the robotic arm.

Compared with the prior art, the technical scheme has the following technical effects: through setting up the couple at the arm, the couple passes the hand-basket and detains and make and place the basket and hang on the arm, simple structure easily realizes, and the couple can easily pass the hand-basket and detain, and then place the basket and install convenient and fast more on the arm.

In one example of the present invention, the placing basket further comprises: the basket is placed, the basket is placed and the basket-carrying frame is placed and is connected, and the basket is placed and is used for holding the embedding box.

Compared with the prior art, the technical scheme has the following technical effects: place the basket and install in placing basket lifting basket frame, the embedding box is placed in placing the basket, places the basket and has a supporting role to the embedding box, and then can realize that the embedding box follows the arm and removes, carries out accurate control to the processing time of embedding box internal tissue.

In one example of the present invention, the basket is provided with mesh plates at both sides thereof, and the mesh plates are installed inside or outside the basket.

Compared with the prior art, the technical scheme has the following technical effects: when the arm will place the basket and put into the container and carry out the decalcification, the decalcification liquid can get into and place the basket in, the decalcification liquid can circulate in the net board, so that the tissue that the decalcification liquid can treat carries out the decalcification processing, the back of decalcification processing, the arm will be placed the basket and take out from the decalcification liquid, place this moment and flow out from the net to remaining decalcification liquid in the basket, make things convenient for the tissue to carry out water washing and fixed processing on next step, it is excessive to avoid causing the tissue decalcification, ensure the film-making quality of decalcification tissue. And the placing basket can be fixed through the inner grid or the outer grid so as to prevent the embedding box from leaking, and the stability of the embedding box is stronger.

In one embodiment of the present invention, the tissue decalcifying apparatus further comprises a box body, wherein the box body is provided with a vertical column in the middle, the container surrounds the vertical column, and the mechanical arm is mounted on the vertical column and can move up and down on the vertical column.

Compared with the prior art, the technical scheme has the following technical effects: the basket is placed to the last suspension of arm, reciprocates through computer programming control arm on the stand, can control the processing time of placing the basket and getting into in the container to and the arm can in time take out from the container placing the basket, gets into next container in order to carry out operation on next step, and the flow is simple, easy to operate, labour saving and time saving, and be difficult to cause the tissue decalcification excessive.

In one example of the utility model, one side of the post is provided with a notch, and the robotic arm is mounted to the notch.

Compared with the prior art, the technical scheme has the following technical effects: the arm can reciprocate in the notch, and labour saving and time saving controls the processing time of embedding box through the lift of control arm, and is more convenient, raises the efficiency.

In one example of the utility model, the top end of the upright is provided with a traction device, and the traction device is connected with the mechanical arm.

Compared with the prior art, the technical scheme has the following technical effects: through draw gear, can realize the arm in the lift of stand department, convenient and fast, and then control the processing time of embedding box in each container more accurately, ensure the film-making quality of decalcification tissue.

In one embodiment of the present invention, the tissue decalcifying apparatus further comprises a flushing device for flushing the embedding cassette.

Compared with the prior art, the technical scheme has the following technical effects: when the embedding box after the decalcification liquid treatment is placed into the container by the mechanical arm, the flushing device can carry out water washing treatment on the embedding box so as to remove the residual decalcification liquid in the tissue and prevent excessive tissue decalcification. And the washing time of the embedding box can be controlled through the programming of the computer host, and the mechanical arm can take out the embedding box in time, so that the next operation is convenient, and the tissue decalcification treatment efficiency is improved.

In one embodiment of the utility model, the housing further comprises a base plate, and the at least one reagent rack is mounted on the base plate, and the base plate can rotate in the housing.

Compared with the prior art, the technical scheme has the following technical effects: at least one deck reagent frame can follow the chassis and rotate, and then conveniently carry out the fluid infusion or change reagent to the liquid in the container on the reagent frame, and the operation is swift more high-efficient.

Drawings

Fig. 1 is a schematic structural diagram of a tissue decalcifying instrument provided by an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a placing basket according to an embodiment of the present invention.

Fig. 3 is a partially enlarged view of the area a in fig. 1.

Fig. 4 is another schematic structural diagram of the placing basket according to the embodiment of the utility model.

Description of reference numerals:

10-a container; 20-placing a basket; 21-placing a basket lifting frame; 22-a basket buckle; 23-placing a basket; 24-a support plate; 25-a base plate; 26-a grid plate; 30-a mechanical arm; 31-hanging hook; 31 a-first portion; 31 b-a second portion; 31 c-a third portion; 40-a box body; 41-upright post; 42-chassis.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.

The first embodiment is as follows:

referring to fig. 1, the present invention provides a tissue decalcifying apparatus, comprising: at least one layer of reagent rack, wherein a plurality of containers 10 are placed on the at least one layer of reagent rack, and the containers 10 are used for containing decalcification solution, stationary solution and water washing solution; a placing basket 20 for placing an embedding box in which tissues to be treated are stored; the robot 30, the placing basket 20 is mounted to the robot 30, and the robot 30 can place the embedding cassette into the container 10 to perform decalcification process on the tissue.

Specifically, the tissue decalcification treatment is performed by a decalcification solution treatment, a water washing treatment and a fixative solution fixation treatment, and the treatment time is limited in each process. The height of the container 10 is higher than that of the embedding box, the container 10 is filled with liquid for tissue decalcification, such as decalcification liquid, water, fixing liquid and the like, and the mechanical arm 30 selects the container to enter according to the tissue decalcification process to perform corresponding treatment. The container 10 has the advantage of deep liquid storage, so that the embedding box can completely enter the container 10, the decalcification liquid can immerse the embedding box, the requirement on the sufficient amount of the decalcification liquid is met, and the containers 10 are enough in number, so that the decalcification conditions for treating different tissue decalcification requirements at the same time or at any time can be met.

Furthermore, the tissue decalcification instrument provided by the utility model is connected with a computer, the computer can set different decalcification programs according to different tissues, flexibly set different decalcification, water washing and fixing time, further control the processing time of the embedding box in the container 10, finely control the tissue decalcification process through the programming of a computer host, and has the advantages of simple flow, difficulty in making mistakes and avoidance of excessive tissue decalcification.

Pathological bone tissue, bone marrow tissue or severely calcified tissue can be processed by decalcification treatment to be processed by conventional flaking, staining, immunological marking and molecular detection. The decalcification reagent, the decalcification time, the washing time, the fixing liquid and the fixing time which are required by different bone tissues with different components and different degrees of ossification are different, so that the factors such as personnel, treatment conditions and the like are not fixed during daily treatment, unstable factors are caused to the tissue decalcification treatment, the problems of excessive decalcification, wrong decalcification fixing reagent and the like are often caused easily, the later-stage dyeing, the immunological typing and the molecular detection cannot be carried out, and the diagnosis and the treatment are seriously influenced. At present, almost no ideal decalcification device special for pathological tissues exists, and the requirements of decalcification conditions of multiple tissues simultaneously or in multiple batches and different conditions of washing and fixing different tissues simultaneously cannot be met. Therefore, the utility model provides a tissue decalcification instrument, which is provided with a plurality of containers 10 for containing liquids of different decalcification liquids and different fixing liquids, wherein the placing basket 20 is placed into the container 10 by the mechanical arm 30, and the embedding box can be immersed by the liquid in the container 10, so that the tissue is decalcification and fixed, time and labor are saved, and manpower is not required.

The tissue decalcification instrument provided by the utility model can simultaneously carry out decalcification, washing and fixing requirements of various tissues under different conditions, has simple and easy-to-use operation process, standardized tissue treatment and good repeatability, and has quality assurance on later-stage staining, immunization and molecular detection of the tissue decalcification.

Example two:

referring to fig. 1 and 2, the basket 20 includes: the basket placing and lifting frame 21 is provided with basket buckles 22 at two ends of the basket placing and lifting frame 21, and the basket placing and lifting frame 21 is mounted on the mechanical arm 30 through the basket buckles 22.

Specifically, the shape of the basket buckle 22 can be circular or square, the mechanical arm 30 penetrates through the basket buckle 22, the placing basket 20 can be hung on the mechanical arm 30, the placing basket 20 moves along with the mechanical arm 30, and the placing basket 20 is simple and convenient to install and easy to operate at the mechanical arm 30.

Example three:

referring to fig. 1 to 3, the robot arm 30 is provided with a hook 31, and the hook 31 passes through the basket holder 22 so that the placing basket 20 is hung from the robot arm 30.

Specifically, the hook 30 includes a first portion 31a, a second portion 31b, and a third portion 31c, the first portion 31a and the third portion 31c respectively form an angle with both ends of the second portion 31b, the third portion 31c passes through the through hole, so that the basket 20 can be hung from the robot arm 30, and the third portion 31c protrudes upward, so that the basket 20 can be prevented from falling.

In another embodiment, the end of the hook 30 is bent to form a certain arc, and the bent portion can rapidly pass through the through hole, so that the placing basket 20 is conveniently installed when being installed on the mechanical arm 30, and the placing basket 20 is not easy to fall off.

Through set up couple 31 on arm 30, simple structure easily realizes, couple 31 can easily pass the hand-basket buckle 22, and then places basket 20 and install convenient and fast more on arm 30.

Example four:

referring to fig. 1 and 2, the placing basket 20 further includes: placing basket 23, placing basket 23 is connected with placing basket lifting frame 21, and placing basket 23 is used for holding the embedding box.

Specifically, the placing basket 23 is installed at the bottom of the placing basket support 21 near the basket buckle 22, and the placing basket 23 includes a support plate 24 and a bottom plate 25. The number of the support plates 24 is two, and the two support plates 24 are symmetrically arranged. The top of the two support plates 24 are installed at both sides of the basket support 21, and the support plates 24 are installed at positions close to the basket buckles 22. The bottom of two backup pads 24 is connected with the both sides of bottom plate 25, and the embedding box is placed on bottom plate 25, and bottom plate 25 has the supporting role to the embedding box, and then can realize that the embedding box follows arm 30 and removes, carries out accurate control to the processing time of the interior tissue of embedding box.

Further, a plurality of grooves are arranged on the bottom plate 25, and the plurality of grooves are distributed on the bottom plate 25 in parallel. For example, when the mechanical arm 30 places the embedding box in the placing basket 20 into the container 10 for decalcification, the decalcification solution can enter the placing basket 20, and the decalcification solution can circulate in the groove, so that the decalcification solution can perform decalcification treatment on the tissue to be treated, after the decalcification treatment, the mechanical arm 30 takes the placing basket 20 out of the decalcification solution, at this time, the residual decalcification solution in the placing basket 20 can flow out along the groove, the tissue is convenient to perform the next treatment, excessive decalcification of the tissue is avoided, and the flaking quality of the decalcification tissue is ensured.

Example five:

referring to fig. 2, mesh plates 26 are provided at both sides of the placing basket 23, and the mesh plates 26 are installed inside or outside the placing basket 23.

Specifically, the grid plate 26 may be an inner grid plate or an outer grid plate.

Specifically, the grid plate 26 is connected to the support plate 24 on both sides, and the grid plate and the bottom plate 25 may be left with a gap.

Further, the neutral position part of net board 26 and bottom plate 25 can be fixed with the rubber band, and the rubber band twines the bottom of net board 26 and the both sides of bottom plate 25 promptly for it is better to the parcel nature of embedding box to place basket 20, and the embedding box is difficult to drop from placing basket 20.

Specifically, referring to fig. 4, the bottom of the grid plate 26 is connected to the bottom plate 25, and at this time, no gap is left between the grid plate 26 and the bottom plate 25, so that the wrapping performance of the placing basket 20 on the embedding box is better, and the embedding box is not easy to fall off from the placing basket 20.

Specifically, referring to fig. 1 again, place the embedding box in placing basket 20, when mechanical arm 30 will place basket 20 and put into container 10 and carry out the decalcification, the decalcification liquid can get into placing basket 20 in, the decalcification liquid can flow through in grid plate 26 to make the decalcification liquid can carry out the decalcification processing to the tissue of handling, the back is handled in the decalcification, mechanical arm 30 will place basket 20 and take out from the decalcification liquid, the remaining decalcification liquid can flow out from grid plate 26 in placing basket 20 this moment, make things convenient for the tissue to carry out next step water washing and fixed processing, avoid causing the tissue decalcification excessive, ensure the film-making quality of decalcification tissue. And the placing basket 23 may be fixed by an inner mesh or an outer mesh to prevent the leakage of the cassette, and thus the stability of the cassette is stronger.

Example six:

referring to fig. 1, the tissue decalcifying instrument further comprises a box 40, wherein a column 41 is arranged in the middle of the box 40, the container 10 surrounds the column 41, the mechanical arm 30 is mounted on the column 41, and the mechanical arm 30 can move up and down on the column 41.

Specifically, the container 10 is placed around the column 41, which facilitates the robotic arm 30 to place the basket 20 into the container 10. The placing basket 20 is hung on the mechanical arm 30, the mechanical arm 30 is controlled to ascend and descend on the upright post 41 through computer host programming, the processing time of the placing basket 20 entering the container 10 can be controlled, the mechanical arm 30 can take the placing basket 20 out of the container 10 in time and enter the next container 10 to be operated on next step, the process is simple, the operation is easy, time and labor are saved, and the tissue decalcification is not easy to cause.

Preferably, the column 41 is rotatable within the housing 40 to facilitate the robotic arm 30 in placing the baskets 20 into different containers 10 for processing.

Example seven:

referring to fig. 1, one side of the column 41 is provided with a notch 42, and the robot arm 30 is mounted to the notch 42.

Specifically, the mechanical arm 30 can move up and down in the notch 42, time and labor are saved, the processing time of the embedding box is controlled by controlling the lifting of the mechanical arm 30, and the method is more convenient and improves the efficiency.

Example eight:

referring to fig. 1, the top end of the column 41 is provided with a traction device, and the traction device is connected with the mechanical arm 30.

Specifically, through draw gear, can realize the lift of arm 30 in stand 41 department, convenient and fast, and then control the processing time of embedding box in each container 10 more accurately, ensure the film-making quality of decalcification tissue.

Example nine:

referring to fig. 1, the tissue decalcifying apparatus further comprises a flushing device for flushing the embedding box.

Specifically, the inside of the tissue decalcification instrument is provided with a water pipe, and when the embedding box processed by the decalcification liquid is placed into the container 10 by the mechanical arm 30, the washing device can carry out washing treatment on the embedding box so as to remove the residual decalcification liquid in the tissue and prevent excessive tissue decalcification. The container 10 is deep in depth and can contain water washing liquid, the water washing time of the embedding box can be controlled through computer host programming, the mechanical arm 30 can take out the embedding box in time, the next operation is convenient to carry out, and the tissue decalcification treatment efficiency is improved.

Example ten:

referring to fig. 1, the housing 40 further includes a bottom plate 42, at least one layer of reagent racks being mounted to the bottom plate 42, the bottom plate 42 being rotatable within the housing 40.

Specifically, at least one layer of reagent frame can follow chassis 42 and rotate, and then conveniently carry out the fluid infusion or change reagent to the liquid in the container 10 on the reagent frame, and the operation is more swift high-efficient.

Specifically, install two-layer reagent frame in the box 40, be reagent frame one and reagent frame two respectively, connect through connecting piece 43 between reagent frame one and the reagent frame two, connecting piece connecting rod in time, connecting plate in time also can to make two-layer reagent frame can rotate in step. Wherein, the first reagent rack is arranged on the chassis 42, one end of the second reagent rack is connected with the top of the box body 40, and the other end is connected with the first reagent rack.

The tissue decalcification instrument provided by the utility model can be obtained by modifying the existing dyeing instrument, and the existing dyeing instrument has a deeper reagent tank and a deeper liquid storage depth and can meet the requirement of sufficient decalcification liquid; the reagent tanks of the dyeing instrument are enough, so that the requirements of simultaneous multi-cylinder different reagents, different treatment time and simultaneous more tissue treatment can be met; the water flushing system has strong capacity and can meet the requirement of thorough flushing of water after tissue decalcification; the device is connected with a computer, can flexibly set different decalcification, washing and fixing time by utilizing the characteristics of flexibility and large use limit value of the dyeing machine, is convenient and easy to improve program setting, and can simultaneously set a plurality of different processing programs to meet the processing requirements of simultaneous or random multi-condition processing. Through transformation, the existing staining instrument can bear the tissue embedding box to carry out decalcification process, the transformation is simple and feasible, the cost is low, the practicability is realized, even if old instruments have new use mission, the daily work flow is simplified, the treatment standardization and consistency of the decalcification tissue are ensured, and the quality of the decalcification tissue slide is ensured.

The utility model discloses also can be for novel manufacturing instrument.

Although the present invention is disclosed above, the present invention is not limited thereto. Various changes and modifications may be effected by one skilled in the art without departing from the spirit and scope of the utility model, as defined in the appended claims.

Claims (10)

1. A tissue decalcifying apparatus, comprising:

the kit comprises at least one layer of reagent rack, a plurality of containers and a plurality of water flushing liquid, wherein the containers are placed on the at least one layer of reagent rack and used for containing decalcification liquid, fixing liquid and water flushing liquid;

the placing basket is used for placing an embedding box, and tissues to be processed are stored in the embedding box;

a robotic arm to which the placement basket is mounted, the robotic arm capable of placing the embedding cassette into the container to decalcify the tissue.

2. The tissue decalcifying apparatus of claim 1, wherein the placement basket comprises: the basket placing device comprises a basket placing lifting frame, wherein basket lifting buckles are arranged at two ends of the basket placing lifting frame, and the basket placing lifting frame is installed on the mechanical arm through the basket lifting buckles.

3. The tissue decalcifying apparatus of claim 2, wherein the robotic arm is provided with a hook that passes through the basket-carrying clasp such that the placing basket is suspended from the robotic arm.

4. The tissue decalcifying apparatus of claim 2, wherein the placement basket further comprises:

place the basket, place the basket with place the basket and carry the basket and put up and be connected, place the basket and be used for holding the embedding box.

5. The tissue decalcifying apparatus of claim 4, wherein the placing basket is provided with mesh plates on both sides thereof, the mesh plates being mounted inside or outside the placing basket.

6. The tissue decalcifying apparatus according to claim 1, further comprising a box, wherein the box is provided with a column at the middle part thereof, the container surrounds the column, the mechanical arm is mounted to the column, and the mechanical arm can move up and down on the column.

7. The tissue decalcifying apparatus of claim 6, wherein a slot is provided on one side of the post, the robotic arm being mounted to the slot.

8. The tissue decalcifying apparatus of claim 6, wherein a traction device is provided at the top end of the post, the traction device being connected to the robotic arm.

9. The tissue decalcifying apparatus of claim 1, further comprising a flushing device for flushing the embedding cassette.

10. The tissue decalcifying apparatus of claim 6, wherein the housing further comprises a chassis, the at least one layer of reagent rack being mounted to the chassis, the chassis being rotatable within the housing.

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