CN216855381U - Bioreactor constant temperature device and system - Google Patents

Abstract

The application relates to the technical field of medical equipment, provides bioreactor constant temperature equipment and system, and the device includes: the constant temperature box is internally provided with a heat conducting medium and is provided with a medium inlet and a medium outlet; at least one bioreactor surrounded by heat-conducting medium is arranged in the incubator; the incubator is provided with mounting ports which correspond to the interfaces on the bioreactor one by one so that the interfaces of the bioreactor penetrate out of the incubator. Through setting up bioreactor in the thermostated container, utilize circulating pump and constant temperature heater to carry out the constant temperature heating to the heat conduction medium that gets into the thermostated container, make bioreactor be in the constant temperature all the time, because bioreactor is whole to be soaked in the heat conduction medium, be heated evenly and stable, guaranteed bioreactor's reaction efficiency.

Description

Bioreactor constant temperature device and system

Technical Field

The utility model belongs to the technical field of medical instruments, and particularly relates to a constant temperature device and system of a bioreactor.

Background

The bioreactor is the core part of the bioartificial liver system acting on cells, and the performance of the bioreactor is directly related to the support and treatment effect of the artificial liver system. The bioreactor which is the most widely researched and applied at present is a hollow fiber type reactor, the structure of the reactor is generally composed of a cylindrical reactor shell and hollow fibers filled in the shell, the hollow fibers are arranged in a bundle, and the wall of the hollow fibers is provided with small holes for exchange. However, the existing bioreactor has some problems, for example, the existing bioreactor only uses a temperature increasing device to increase the temperature, and cannot heat the bioreactor at constant temperature, the heating of the bioreactor is unstable and unbalanced, and the cells in the bioreactor cannot be always in a high activity state, which affects the efficiency of the bioartificial liver system.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problems that the existing temperature increasing device heats a bioreactor unevenly, is unstable in heating and cannot keep cells in the bioreactor in a high-activity state all the time, and provides a constant temperature device and a constant temperature system for the bioreactor.

In a first aspect, there is provided a bioreactor thermostat comprising:

the heat conduction device comprises a constant temperature box, a heat conduction medium, a medium inlet and a medium outlet, wherein the constant temperature box is internally provided with the heat conduction medium, the medium inlet is used for inputting the heat conduction medium into the constant temperature box, and the medium outlet is used for extracting the heat conduction medium from the constant temperature box;

at least one bioreactor surrounded by the heat conducting medium is placed in the incubator; the incubator is provided with mounting ports which are in one-to-one correspondence with the interfaces on the bioreactor, so that the interfaces of the bioreactor penetrate out of the incubator.

Further, the incubator comprises an incubator body and an incubator cover, wherein the incubator body is detachably connected with the incubator cover; the interface on the bioreactor comprises a plasma inlet, a plasma outlet, a nutrient solution inlet and a nutrient solution outlet; the mounting port includes a plurality of nutrient solution ports and a plurality of plasma ports.

Still further, the bioreactor is horizontally arranged in the box body; the nutrient solution port is arranged on the box cover, and the blood plasma port is arranged at the joint of the box cover and the box body; the two nutrient solution ports are respectively matched with the nutrient solution inlet and the nutrient solution outlet, and the two plasma ports are respectively matched with the plasma inlet and the plasma outlet.

Preferably, a sealing ring is sleeved on the interface of the bioreactor and penetrates through the mounting port, and the sealing ring is connected with the mounting port in a sealing manner.

The beneficial effects of the above preferred scheme are: through setting up the sealing washer on the interface, make sealing washer and installing port sealing connection, guaranteed the leakproofness of being connected of bioreactor and thermostated container, avoid the heat conduction medium in the thermostated container to spill over from the installing port, also avoid the nutrient solution in the bioreactor to reveal to the thermostated container in from the kneck, in addition, also in time discover the problem of revealing of bioreactor.

Further, a fixing part for fixing the bioreactor is arranged in the incubator.

Fix bioreactor through the mounting, can avoid bioreactor to take place the displacement under the effect of heat conduction medium, also can be through the injecing to bioreactor position, make a plurality of bioreactors evenly arrange in the thermostated container, each bioreactor is heated evenly.

Preferably, the fixing member is a snap or a clip.

In one embodiment, the box body comprises a bottom plate and an inner barrel, a middle barrel and an outer barrel which are concentric and have different diameters and are erected on the bottom plate; the inner cavity of the inner cylinder is a medium inlet cavity, and the area between the inner cylinder and the middle cylinder is a pipeline cavity; the area between the middle barrel and the outer barrel is a heating cavity; the plurality of bioreactors are uniformly distributed in the heating cavity along the circumferential direction of the middle cylinder, and a nutrient solution inlet and a nutrient solution outlet of each bioreactor penetrate through the middle cylinder and extend into the pipeline cavity; the plasma inlet of the bioreactor passes through the box cover, and the plasma outlet of the bioreactor passes through the bottom plate; the medium inlet comprises a first inlet and a second inlet, the first inlet penetrates through the box cover to be communicated with the medium inlet cavity, one end of the second inlet extends into the medium inlet cavity, and the other end of the second inlet extends into the heating cavity and is used for conveying heat-conducting medium into the heating cavity; the medium outlet is arranged on the outer cylinder and used for discharging heat-conducting media in the heating cavity.

The beneficial effects of the above embodiment are: the box body is arranged into the inner cylinder, the middle cylinder and the outer cylinder, so that the plurality of bioreactors can be uniformly heated.

Further, heating intracavity is provided with plum blossom shape radome fairing with one heart, the inner chamber of radome fairing is formed with a plurality of edges that are linked together the circumference evenly distributed's of well section of thick bamboo heat preservation chamber, still evenly be provided with a plurality of intercommunications on the radome fairing heat preservation chamber with the overflow mouth in heating chamber, stretching into of second entry one end in the heating intracavity is located the heat preservation intracavity, heat preservation intracavity is provided with bioreactor.

The beneficial effects of the further scheme are as follows: through the radome fairing that has the effect of gathering heat in heating intracavity setting to form the heat preservation chamber, make the constant temperature heating effect that is located the bioreactor of heat preservation intracavity better, the intensification is rapid, and the cooling is slow, has improved bioreactor's reaction efficiency.

Further preferably, the fairing is formed by a plurality of arc flow baffles in an enclosing mode.

In a second aspect, a bioreactor constant temperature system is provided, which includes the bioreactor constant temperature device, and further includes:

the circulating pipe is communicated with the medium inlet and the medium outlet and is communicated with the constant temperature box to form a circulating loop for the heat-conducting medium to circulate;

the circulating pump is arranged on the circulating pipe to drive the heat-conducting medium to circularly flow along the circulating loop; and

and the constant temperature heater is arranged on the circulating pipe and used for heating the heat conducting medium.

The utility model has the beneficial effects that: through setting up bioreactor in the thermostated container, utilize circulating pump and constant temperature heater to carry out the constant temperature heating to the heat conduction medium that gets into the thermostated container, make bioreactor be in the constant temperature state all the time, because bioreactor is whole to be surrounded by heat conduction medium, it is even and stable to be heated, makes the cell in the bioreactor remain the high activity state all the time, has guaranteed bioreactor's reaction efficiency.

Drawings

FIG. 1 is a schematic diagram of the structure of one embodiment of the isothermal system of the bioreactor of the present invention.

FIG. 2 is a schematic sectional view A-B-A of the thermostat device of the bioreactor in FIG. 1.

FIG. 3 is a schematic perspective view of a bioreactor and a buckle disposed thereon in FIG. 2.

FIG. 4 is a schematic perspective view of another embodiment of the thermostat device for a bioreactor according to the present invention.

Fig. 5 is a schematic sectional top view of the case of fig. 4.

FIG. 6 is a schematic perspective view of a bioreactor and a buckle disposed thereon in FIG. 5.

FIG. 7 is a schematic view of the structure of the heat transfer medium flow trace of FIG. 5 with the bioreactor removed.

In the figure, 1-circulation pipe; 2-a media inlet; 2.1-a first inlet; 2.2-a second inlet; 3-a medium outlet; 4-a circulating pump; 5-constant temperature heater; 6, a box body; 7-box cover; 8-a shell; 9-plasma inlet; 10-plasma outlet; 11-nutrient solution inlet; 12-nutrient solution outlet; 14-buckling; 15-a base plate; 16-inner cylinder; 17-a middle cylinder; 18-an outer barrel; 19-media entry chamber; 20-a line cavity; 21-heating chamber; 22-a fairing; 23-heat preservation cavity; 24-an overflow port; 25-flow baffle.

Detailed Description

The utility model is described in further detail below with reference to figures 1 to 7 and the specific embodiments.

Example 1

A bioreactor constant temperature system as shown in FIGS. 1 to 3 includes a bioreactor constant temperature device, a circulation pipe 1, a circulation pump 4, and a constant temperature heating device.

Bioreactor constant temperature equipment includes the thermostated container, is provided with heat conduction medium in the thermostated container, is provided with medium entry 2 and medium export 3 on the thermostated container, and medium entry 2 is used for to the heat conduction medium of inputing in the thermostated container, and medium export 3 is used for taking out the heat conduction medium in the thermostated container.

At least one bioreactor surrounded by heat-conducting media is placed in the incubator; the incubator is provided with mounting ports which correspond to the interfaces on the bioreactor one by one so that the interfaces of the bioreactor penetrate out of the incubator.

The circulation pipe 1 is communicated with the medium inlet 2 and the medium outlet 3 and is communicated with the thermostat to form a circulation loop for the heat-conducting medium to circulate.

The circulation pump 4 is provided on the circulation pipe 1 to drive the heat transfer medium to circulate along the circulation loop.

The constant temperature heater 5 is provided on the circulation pipe 1 for heating the heat transfer medium.

The heat conducting medium in this embodiment is water, and may be other heat conducting liquid or gas, where the heat conducting medium may be a warm air flow heated in advance when the heat conducting medium is gas.

The constant temperature heater 5 and the circulating pump 4 in this embodiment may be any conventional ones.

The constant temperature box comprises a box body 6 and a box cover 7, wherein the box body 6 is detachably connected with the box cover 7; the bioreactor comprises a shell 8, and the interface on the bioreactor comprises a plasma inlet 9, a plasma outlet 10, a nutrient solution inlet 11 and a nutrient solution outlet 12 which are arranged on the shell 8. Wherein the plasma inlet 9 and the plasma outlet 10 are arranged at both axial ends of the housing 8. A nutrient solution inlet 11 and a nutrient solution outlet 12 are provided at the side ends of the housing 8.

The mounting port on the incubator includes a plurality of nutrient solution ports and a plurality of plasma ports.

The shell 8 of the bioreactor is horizontally arranged in the box body 6; the nutrient solution port is arranged on the box cover 7, and the plasma port is arranged at the joint of the box cover 7 and the box body 6. The two nutrient solution ports are respectively matched with the nutrient solution inlet 11 and the nutrient solution outlet 12, and the two plasma ports are respectively matched with the plasma inlet 9 and the plasma outlet 10. When the number of the bioreactors is more, the corresponding nutrient solution port and the corresponding plasma port are correspondingly increased and arranged at corresponding positions.

The interface of the bioreactor is sleeved with a sealing ring and penetrates through the mounting opening, and the sealing ring is connected with the mounting opening in a sealing way. Through setting up the sealing washer on the interface, make sealing washer and installing port sealing connection, guaranteed the leakproofness of being connected of bioreactor and thermostated container, avoid the heat conduction medium in the thermostated container to spill over from the installing port, also avoid the nutrient solution in the bioreactor to reveal to the thermostated container in from the kneck, in addition, also in time discover the problem of revealing of bioreactor.

A fixing part for fixing the bioreactor is arranged in the constant temperature box. The securing member is a snap 14 or clip. The preferred fastener in this embodiment is a clasp 14. Fix bioreactor through the mounting, can avoid bioreactor to take place the displacement under the effect of heat conduction medium, also can be through the injecing to bioreactor position, make a plurality of bioreactors evenly arrange in the thermostated container, each bioreactor is heated evenly.

It should be noted that, after the bioreactor of this embodiment is immersed in the incubator, since the bioreactor is an integrally formed structure and the interfaces connecting with the bioreactor system (existing system) are all located outside the incubator, the incubator and the heat transfer medium inside the incubator will not affect the use of the bioreactor. Namely, the use of the incubator and the bioreactor do not interfere with each other.

Because the whole bioreactor is surrounded by the heat conducting medium, the heating is uniform and stable, the cells in the bioreactor are always kept in a high activity state, and the reaction efficiency of the bioreactor is ensured.

Example 2

As shown in fig. 4 to 7, the bioreactor thermostat of the present example has substantially the same structure as that of example 1 except that:

the case 6 of the present example is cylindrical, and the case 6 of the example 1 is cubic. The box body 6 of the embodiment comprises a bottom plate 15, an inner cylinder 16, a middle cylinder 17 and an outer cylinder 18 which are concentric with each other and have different diameters and are erected on the bottom plate 15; the inner cavity of the inner cylinder 16 is a medium inlet cavity 19, and the area between the inner cylinder 16 and the middle cylinder 17 is a pipeline cavity 20; the area between the middle barrel 17 and the outer barrel 18 is a heating cavity 21; a plurality of bioreactors are evenly distributed in the heating cavity 21 along the circumference of the middle barrel 17, a nutrient solution inlet 11 and a nutrient solution outlet 12 of each bioreactor penetrate through the middle barrel 17 and extend into the pipeline cavity 20, pipeline penetrating ports are further formed in positions, corresponding to the pipeline cavity 20, of the box cover 7, pipelines of the biological reaction system penetrate through the corresponding pipeline penetrating ports and then are communicated with the nutrient solution inlet 11 or the nutrient solution outlet 12, and therefore the biological reaction system can provide nutrient solution for the bioreactors. The plasma inlet 9 of the bioreactor passes through the lid 7 and the plasma outlet 10 of the bioreactor passes through the base 15. The medium inlet 2 of the present embodiment comprises a first inlet 2.1 and a second inlet 2.2. The first inlet 2.1 penetrates through the box cover 7 to be communicated with the medium inlet cavity 19, one end of the second inlet 2.2 extends into the medium inlet cavity 19, and the other end of the second inlet 2.2 extends into the heating cavity 21 to convey the heat-conducting medium into the heating cavity 21. With the cooperation of the first inlet 2.1 and the second inlet 2.2, an external heat-conducting medium is introduced into the heating chamber 21. The medium outlet 3 is provided on the outer cylinder 18 for discharging the heat transfer medium in the heating chamber 21.

The heating chamber 21 is internally concentrically provided with a quincunx fairing 22, the inner cavity of the fairing 22 is formed with a plurality of communicated heat preservation chambers 23 which are uniformly distributed along the circumference of the middle cylinder 17, the fairing 22 is also uniformly provided with a plurality of overflow ports 24 which are communicated with the heat preservation chambers 23 and the heating chamber 21, one end of the second inlet 2.2 which extends into the heating chamber 21 is positioned in the heat preservation chamber 23, and the heat preservation chamber 23 is internally provided with a bioreactor. The fairing 22 is formed by a plurality of arcuate baffles 25.

The buckle 14 is fixed on the outer wall of the middle cylinder 17.

In this embodiment, there are four insulated chambers 23, and each insulated chamber 23 has a bioreactor therein. There are one first inlet 2.1 and four second inlets 2.2, and there are one second inlet 2.2 at the juncture of every two adjacent chambers 23 and correspondingly there are four media outlets 3. The arrows in fig. 7 indicate the direction of flow of the heat transfer medium. When the system of the embodiment operates, the heat-conducting medium heated by the constant temperature heater 5 is pumped into the medium inlet cavity 19 (the medium inlet cavity 19 is communicated with the circulating pipe 1), the heat-conducting medium in the medium inlet cavity 19 uniformly flows into the corresponding four heat preservation cavities 23 from the four second inlets 2.2, and rapidly wraps the bioreactor in the heat preservation cavities 23, then flows out to the heating cavity 21 from the overflow port 24, and finally flows back to the circulating pump 4 after flowing out from the medium outlet 3, so that the heat-conducting medium is reheated by the constant temperature heater 5 and then is injected into the box body 6 again.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the utility model may be made by those skilled in the art without departing from the principle of the utility model.

Claims (10)

1. A bioreactor thermostat, comprising:

the heat conduction device comprises a constant temperature box, a heat conduction medium, a medium inlet and a medium outlet, wherein the constant temperature box is internally provided with the heat conduction medium, the medium inlet is used for inputting the heat conduction medium into the constant temperature box, and the medium outlet is used for extracting the heat conduction medium from the constant temperature box;

at least one bioreactor surrounded by the heat-conducting medium is placed in the incubator; the incubator is provided with mounting ports which are in one-to-one correspondence with the interfaces on the bioreactor, so that the interfaces of the bioreactor penetrate out of the incubator.

2. The bioreactor thermostat of claim 1, wherein the incubator comprises a box body and a box cover, the box body being removably connected to the box cover; the interface on the bioreactor comprises a plasma inlet, a plasma outlet, a nutrient solution inlet and a nutrient solution outlet; the mounting port includes a plurality of nutrient solution ports and a plurality of plasma ports.

3. The bioreactor thermostat of claim 2, wherein the bioreactor is horizontally disposed within the tank; the nutrient solution port is arranged on the box cover, and the plasma port is arranged at the joint of the box cover and the box body; the two nutrient solution ports are respectively matched with the nutrient solution inlet and the nutrient solution outlet, and the two plasma ports are respectively matched with the plasma inlet and the plasma outlet.

4. The thermostat device of the bioreactor as claimed in claim 1, wherein a sealing ring is sleeved on the interface of the bioreactor and penetrates through the mounting port, and the sealing ring is connected with the mounting port in a sealing manner.

5. Bioreactor thermostat according to claim 1, characterized in that inside the incubator there are provided fixing means for fixing the bioreactor.

6. Bioreactor thermostat according to claim 5, characterized in that the fixing means are snaps or clips.

7. The bioreactor thermostat of claim 2, wherein the box body comprises a bottom plate and an inner barrel, a middle barrel and an outer barrel which are concentric and have different diameters and are erected on the bottom plate; the inner cavity of the inner cylinder is a medium inlet cavity, and the area between the inner cylinder and the middle cylinder is a pipeline cavity; the area between the middle barrel and the outer barrel is a heating cavity; the plurality of bioreactors are uniformly distributed in the heating cavity along the circumferential direction of the middle cylinder, and a nutrient solution inlet and a nutrient solution outlet of each bioreactor penetrate through the middle cylinder and extend into the pipeline cavity; the plasma inlet of the bioreactor passes through the box cover, and the plasma outlet of the bioreactor passes through the bottom plate; the medium inlet comprises a first inlet and a second inlet, the first inlet penetrates through the box cover to be communicated with the medium inlet cavity, one end of the second inlet extends into the medium inlet cavity, and the other end of the second inlet extends into the heating cavity and is used for conveying heat-conducting medium into the heating cavity; the medium outlet is arranged on the outer cylinder and used for discharging heat-conducting media in the heating cavity.

8. The bioreactor thermostat device according to claim 7, wherein a quincunx fairing is concentrically arranged in the heating cavity, a plurality of communicated heat preservation cavities are formed in an inner cavity of the fairing and are uniformly distributed along the circumferential direction of the middle cylinder, a plurality of overflow ports communicated with the heat preservation cavities and the heating cavity are uniformly formed in the fairing, one end of the second inlet extending into the heating cavity is located in the heat preservation cavity, and the bioreactor is arranged in the heat preservation cavity.

9. The bioreactor thermostat of claim 8, wherein the cowling is formed by a plurality of arcuate baffles.

10. A bioreactor thermostat system comprising the bioreactor thermostat device of any one of claims 1 to 9, further comprising:

the circulating pipe is communicated with the medium inlet and the medium outlet and is communicated with the constant temperature box to form a circulating loop for the heat-conducting medium to circulate;

the circulating pump is arranged on the circulating pipe to drive the heat-conducting medium to circularly flow along the circulating loop; and

and the constant temperature heater is arranged on the circulating pipe and used for heating the heat conducting medium.

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