CN216816453U - Double-color-development microorganism detection device based on magnetic field control and combined detection equipment - Google Patents

Abstract

The utility model provides a magnetic field control-based double-color-development microorganism detection device and combined detection equipment, relates to the technical field of biological detection, and solves the technical problem that a novel corona virus detection kit with short detection time and high sensitivity is absent in the prior art. The device comprises an accommodating cavity, a magnetic part and a light source assembly, wherein a first detection cavity and a second detection cavity which are communicated with each other are formed in the accommodating cavity, the magnetic part is positioned at the position of the first detection cavity, and the light source assembly is used for irradiating the position of the first detection cavity and the position of the second detection cavity.

Description

Double-color-development microorganism detection device based on magnetic field control and combined detection equipment

Technical Field

The utility model relates to the technical field of biological detection, in particular to a magnetic field control-based double-color-development microorganism detection device and combined detection equipment.

Background

Pathogenic microorganisms refer to microorganisms, or pathogens, that can invade the body, causing infection and even infectious disease. Among pathogens, the most harmful are bacteria and viruses. The pathogenic microorganisms fight against the human being for a long time and complexly, the pathogenic microorganisms have the greatest culprit of enhancing the virulence or pathogenicity of the pathogenic microorganisms through continuous reproduction, mutation and evolution, and the human being kills, discharges and defeats the invaded pathogenic microorganisms through a powerful immune system of the human body. After the pathogens invade the human body, the human body is the place where the pathogens live, and is medically called as the host of the pathogens. The pathogenic agent grows and breeds in the host, releases toxic substances and the like to cause pathological changes of different degrees in the body, and the process is called infection. However, unlike the culture medium for artificially cultured bacteria, human or animal can cause death of the organism by allowing pathogens to grow and propagate indefinitely. After the pathogens invade the human body, the infection occurs, and simultaneously, the immune system of the human body can be stimulated to generate a series of immune responses to fight against the infection, which is called immunity. In addition to the host's own strength, the synergistic effects of effective antibacterial drugs and other measures are also essential, and most diseases can be treated quickly by the combined action of various factors. By virtue of the continuing development of scientific progress, microorganisms harmful to humans and animals are always continuously controlled and destroyed. Therefore, the method has important significance for rapidly and accurately detecting the type of the infected pathogenic microorganism at the initial stage of infection and then pertinently selecting the medicine or not using the medicine for intervention.

Such as novel coronavirus, human papilloma virus, influenza a virus, influenza b virus, human immunodeficiency virus, etc., all will produce different degrees of damage to the organism after infection, even lose life. Therefore, the method can accurately and quickly diagnose the pathogenic microorganism species and has great significance for clinical treatment. However, currently, the detection means commonly used, such as an enzyme linked immunosorbent assay kit, a chemiluminescence assay kit, and a fluorescence immunoassay kit, are different in the indicator reagent, and the kit needs to be matched with different instruments to read and analyze signals, so that the detection result can be obtained. Not only wastes time and labor, but also has higher cost, and is widely applied to hospitals and detection companies. The cost per test is high for the individual. Therefore, a detection product with short detection time, high sensitivity and suitable for patient self-test is urgently needed in the market.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a magnetic field control-based double-color-development microorganism detection device and combined detection equipment, and aims to solve the technical problem that a pathogenic microorganism detection device with short detection time and high sensitivity is lacked in the prior art. The technical effects that can be produced by the preferred technical scheme in the technical schemes provided by the utility model are described in detail in the following.

In order to achieve the purpose, the utility model provides the following technical scheme:

the utility model provides a magnetic field control-based double-color-development microorganism detection device, which comprises an accommodating cavity, a magnetic part and a light source component, wherein,

the accommodating cavity is internally provided with a first detection cavity and a second detection cavity which are communicated, the magnetic component is positioned at the position of the first detection cavity, and the light source component is used for irradiating the positions of the first detection cavity and the second detection cavity.

Optionally, the light source assembly is an external light source for exciting fluorescein isothiocyanate to develop color.

Optionally, the light source assembly is a light wave having a wavelength of 488 nm.

Optionally, the magnetic component is mounted on the inner wall of the housing;

the shell is provided with an observation window which is a transparent body or an opening.

Optionally, the observation window includes a first observation window and a second observation window, the first observation window corresponds to the first detection cavity, and the second observation window corresponds to the second detection cavity.

Optionally, the light source module further comprises a power button, the light source module is a light source strip, the light source strip is installed on the port of the shell, the power button is installed on the outer side wall of the shell, and the light source strip is electrically connected with the power button.

Optionally, a partition plate and a waste liquid collecting tank are arranged in the shell, and the waste liquid collecting tank and the accommodating cavity are respectively arranged at two ends of the partition plate;

the partition board is provided with a first through hole, or the outer wall of the shell is provided with a second through hole, and the second through hole is communicated with the waste liquid collecting tank.

The utility model provides combined detection equipment which comprises a pretreatment vessel, a reaction vessel and the double-color-developing microorganism detection device based on magnetic field control, wherein one or more receptor reagents which are labeled by fluorescein isothiocyanate and can be identified by pathogenic microorganisms, namely FITC-receptor reagents, are sealed in the pretreatment vessel, one or more competitive reagents which are labeled by magnetic beads and competitively bind with the receptor reagents with the microorganisms to be detected, namely magnetic bead-competitive reagents, are sealed in the reaction vessel, and the reaction vessel can be inserted into the double-color-developing microorganism detection device based on magnetic field control.

The device can be used in cooperation with a detection reagent, preferably, the detection reagent can be a fluorescein isothiocyanate labeled receptor reagent (FITC-receptor reagent) capable of being specifically identified by pathogenic microorganisms and a magnetic bead labeled competition reagent (magnetic bead-competition reagent) capable of competitively binding with the receptor reagent with microorganisms to be detected. When the device is used for detecting a sample to be detected, the sample to be detected and the FITC-receptor reagent are uniformly mixed, then the mixture and the magnetic bead-competitive reagent are uniformly mixed to form a mixture to be detected, the mixture is placed into the accommodating cavity 1, and under the action of the magnetic component 2, a complex formed by the combination of the magnetic bead-competitive reagent and the FITC-receptor reagent or the magnetic bead-competitive reagent can be gathered at the position of the first detection cavity 11. The specific detection process is as follows:

if the sample is negative, namely the sample does not contain the microorganism to be detected, the FITC-receptor reagent is combined with the competitive reagent in the magnetic bead-competitive reagent to form a FITC-receptor reagent-competitive reagent-magnetic bead complex. Under the action of the magnetic component, a FITC-receptor reagent-competition reagent-magnetic bead complex is concentrated in the first detection cavity, and after the light source component irradiates, the fluorescence color is displayed in the first detection cavity; and the second detection cavity is colorless after the light source component irradiates because of no chromogenic substrate.

If the sample is positive, the microorganism to be detected in the sample is combined with the FITC-receptor reagent, and the microorganism to be detected is excessive, the FITC-receptor reagent is completely occupied by the microorganism to be detected to form a microorganism-receptor reagent-FITC complex to be detected, the microorganism-receptor reagent-FITC complex is gathered in the second detection cavity, and the magnetic bead-competitive reagent is gathered in the first detection cavity under the action of the magnetic field. Under the irradiation of the light source component, the first detection cavity has no fluorescence color development, and the second detection cavity has fluorescence color development. If the content of the microorganisms to be detected in the positive sample is low, the FITC-receptor reagent is partially occupied, so that a microorganism-receptor reagent-FITC complex to be detected is formed and gathered in the second detection cavity; the unoccupied part is combined with the magnetic bead-competitive reagent to form a FITC-receptor reagent-competitive reagent-magnetic bead complex, and the FITC-receptor reagent-competitive reagent-magnetic bead complex is gathered in the first detection cavity under the action of a magnetic field. Under the illumination of light source subassembly, first detection chamber and second detect the chamber and all have fluorescence development, and the time spent is few, and sensitivity is high simultaneously, has solved to lack one kind among the prior art and has had the short, the high technical problem who has the new coronavirus detection kit of sensitivity simultaneously.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram of a magnetic field control-based dual-color microorganism detection device under the irradiation of an external light source;

FIG. 2 is a schematic structural diagram of a magnetic field control-based dual-color-development microorganism detection device according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view of a magnetic field control-based dual-color-development microorganism detection device according to an embodiment of the present invention.

Wherein, 1, accommodating cavity; 11. a first detection chamber; 12. a second detection chamber; 2. a magnetic member; 3. an external light source; 4. a housing; 41. an observation window; 411. a first observation window; 412. a second observation window; 42. a partition plate; 421. a first through hole; 43. a waste liquid collecting tank; 5. a light source strip; 6. a power button.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the utility model, and not restrictive of the full scope of the utility model. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

In the description of the present invention, it is to be noted that "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing and simplifying the description, but do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated, and thus are not to be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood as appropriate to those of ordinary skill in the art.

Example 1:

the utility model provides a magnetic field control-based double-color-development microorganism detection device which comprises an accommodating cavity 1, a magnetic part 2 and a light source assembly, wherein a first detection cavity 11 and a second detection cavity 12 which are communicated with each other are formed in the accommodating cavity 1, the magnetic part 2 is positioned at the position of the first detection cavity 11, and the light source assembly is used for irradiating the positions of the first detection cavity 11 and the second detection cavity 12. When the device is used for detecting a sample to be detected, the sample to be detected and the FITC-receptor reagent are uniformly mixed, then the mixture is uniformly mixed with the magnetic bead-competitive reagent to form a mixed solution to be detected and is placed into the accommodating cavity 1, under the action of the magnetic component 2, a complex formed by the combination of the magnetic bead-competitive reagent and the FITC-receptor reagent and the complex formed by the combination of the magnetic bead-competitive reagent and the FITC-receptor reagent can be gathered at the position of the first detection cavity 11, and the complex formed by the combination of the magnetic bead-competitive reagent and the FITC-receptor reagent can emit fluorescence color under the excitation of the light source component. Wherein, the fluorescein isothiocyanate can emit green fluorescence under the irradiation of a light source with a 488nm wave band.

The double-color-development microorganism detection device based on magnetic field control can be used for detecting novel coronavirus. The specific process is as follows: the method comprises the steps of firstly mixing fluorescein isothiocyanate labeled angiotensin converting enzyme II (abbreviated as FITC-ACE2) with an RBD (abbreviated as RBD) domain on novel coronavirus spike protein S in a sample to be detected, and mixing a primary mixed solution formed by the fluorescein isothiocyanate labeled angiotensin converting enzyme II and the RBD domain on the novel coronavirus spike protein S (abbreviated as magnetic bead-RBD) marked by the magnetic bead to form a mixed solution to be detected. If the sample contains RBD protein, the RBD protein is combined with FITC-ACE2 to form RBD-ACE2-FITC, the RBD-ACE2-FITC is gathered in a sample observation area (namely the second detection cavity 12), and fluorescence color development is realized under the irradiation of a light source; it should be noted here that if the sample contains a small amount of RBD protein, i.e., the infection is mild, FITC-ACE2 is not completely occupied. The redundant FITC-ACE2 is combined with magnetic bead-RBD, and is gathered in a magnetic observation area (namely the first detection cavity 11) to emit fluorescence color development under the irradiation of a light source; if the RBD protein content in the sample is high, namely severe infection, the magnetic observation area has no fluorescence coloration. If the sample is negative, FITC-ACE2 is combined with magnetic bead-RBD, and the mixture is gathered in a magnetic observation area and emits fluorescence color under the irradiation of a light source. Namely:

because the occupancy rate of FITC-ACE2 is used for determining the concentration of RBD protein in a sample, the infection condition of a patient needs to be further judged clinically by combining clinical diagnosis of a doctor.

The methods for detecting other microorganisms using the detection device provided by the present application are the same as above, but are not exhaustive.

Example 2:

in an alternative embodiment, the light source assembly is an external light source 3, the wavelength of the light of the external light source 3 is 488nm, the external light source 3 can irradiate the first detection cavity 11 and the second detection cavity 12, and the angiotensin-converting enzyme II with the fluorescein isothiocyanate label can be excited under the irradiation of the external light source 3. The external light source 3 is a separate component, the external light source 3 does not need to be installed on the housing 4, and when detection is carried out, the external light source 3 only needs to be irradiated towards the accommodating cavity 1.

Example 3:

as an optional embodiment, the detection device further comprises a housing 4, the accommodating cavity 1 is located inside the housing 4, the first detection cavity 11 and the second detection cavity 12 are also located inside the housing 4, the magnetic component 2 is mounted on the inner wall of the housing 4, and the magnetic component 2 is located in the range of the first detection cavity 11;

be provided with observation window 41 on the casing 4, can look over the mixed liquid that awaits measuring in holding chamber 1 through observation window 41. The observation window 41 is a relatively large window through which the first detection chamber 11 and the second detection chamber 12 can be observed simultaneously; alternatively, the observation window 41 includes a first observation window 411 and a second observation window 412, the first observation window 411 corresponds to the first detection chamber 11, and the second observation window 412 corresponds to the second detection chamber 12.

As optional implementation mode, still include power button 6, the light source subassembly is light source lamp area 5, the wavelength of the light wave of light source lamp area 5 is 488nm, light source lamp area 5 is installed on the port of casing 4, first detection chamber 11 and second detection chamber 12 are located same one side of light source lamp area 5, power button 6 installs on the lateral wall of casing 4, light source lamp area 5 and power button 6 electricity are connected, power button 6 can control the lamp in light source lamp area 5 and the lamp goes out.

Example 4:

as an alternative embodiment, a partition plate 42 and a waste liquid collecting tank 43 are arranged in the housing 4, and two ends of the partition plate 42 are respectively the waste liquid collecting tank 43 and the accommodating cavity 1; be provided with first through-hole 421 on the baffle 42, after the mixed liquid that awaits measuring in holding chamber 1 detects the completion, can send into waste liquid collecting tank 43 through first through-hole 421 with its mixed liquid that awaits measuring, perhaps be provided with the second through-hole on the outer wall of casing 4, the second through-hole is linked together with waste liquid collecting tank 43. First through-hole 421 and second through-hole all are provided with can dismantle the end cap, in order can recycle, the reaction ware can stretch into and hold in chamber 1 and the reaction ware can hold the mixed liquid that awaits measuring, after detecting the completion, the mixed liquid that awaits measuring in the reaction ware can enter into waste liquid collecting tank 43 through first through-hole 421 or second through-hole, perhaps the reaction ware can enter into waste liquid collecting tank 43 with the mixed liquid that awaits measuring through first through-hole 421 with the help of auxiliary pipeline, and through using the reaction ware, make the mixed liquid that awaits measuring can not remain and hold in chamber 1.

Example 5:

the utility model provides combined detection equipment which comprises a pretreatment vessel, a reaction vessel and a double-color-developing microorganism detection device based on magnetic field control, wherein one or more receptor reagents which are labeled by fluorescein isothiocyanate and can be identified by pathogenic microorganisms, namely FITC-receptor reagents, are sealed in the pretreatment vessel, one or more competitive reagents which are labeled by magnetic beads and competitively combine with the receptor reagents with microorganisms to be detected, namely magnetic bead-competitive reagents, are sealed in the reaction vessel, the reaction vessel can be inserted into the double-color-developing microorganism detection device based on the magnetic field control, and the reaction vessel is made of transparent materials.

As one embodiment, the detection process of the combined detection device provided by the utility model comprises the following steps:

a. adding a sample to be detected into a pretreatment vessel sealed with a FITC-receptor reagent, fully and uniformly mixing, and reacting for 3 minutes;

b. pouring the mixed solution in the pretreatment vessel into a reaction vessel sealed with the magnetic beads and the competitive reagent, and reversing and uniformly mixing;

c. inserting the reaction vessel into the accommodating cavity 1, and turning on the power button 6;

d. and interpreting the result through the color development conditions of the first observation window and the second observation window.

It should be noted that FITC-receptor reagent can be used in an amount that is occupied by the bead-competitor reagent.

Example 6:

when the sample to be detected is positive:

A. when a sample to be detected contains high-concentration microorganisms to be detected, the FITC-receptor reagent can be completely occupied by the microorganisms to be detected, when the sample is subjected to the magnetic attraction effect of the magnetic component 2, the magnetic bead-competitive reagent is adsorbed into the first detection cavity 11, a green magnetic bead band cannot be formed under the illumination, the FITC-receptor reagent-microorganisms to be detected are located in the second detection cavity 12 in a free state, and the liquid is green under the illumination.

B. When the sample contains low-concentration microorganisms to be detected, the FITC-receptor reagent is partially occupied by the microorganisms to be detected, and the non-occupied FITC-microorganisms to be detected are combined with the magnetic bead-competition reagent. When the magnetic component 2 is magnetically attracted, the FITC-receptor reagent and the magnetic bead-competition reagent combination are gathered in the first detection cavity 11, and a green magnetic bead band is formed under illumination; the combination of FITC-receptor reagent and the microorganism to be detected is gathered in the second detection chamber 12, and the liquid is green under illumination.

When the sample to be detected is negative:

when no microorganism to be detected exists in a sample to be detected, the FITC-receptor reagent is combined with the magnetic bead-competition reagent, and when the sample to be detected is magnetically attracted by the magnetic component 2, the sample to be detected is gathered in the first detection cavity 11 to form a green magnetic bead band under illumination; and the liquid in the second detection cavity 12 is colorless under illumination.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (8)

1. A double-color-development microorganism detection device based on magnetic field control is characterized by comprising an accommodating cavity (1), a magnetic part (2) and a light source component, wherein,

a first detection cavity (11) and a second detection cavity (12) which are communicated with each other are formed in the accommodating cavity (1), the magnetic component (2) is located at the position of the first detection cavity (11), and the light source component is used for irradiating the positions of the first detection cavity (11) and the second detection cavity (12).

2. The magnetic field control-based dual color-development microorganism detection device according to claim 1, wherein the light source assembly is an external light source (3), and the external light source (3) is used for exciting fluorescein isothiocyanate to develop color.

3. The magnetic field control-based dual color-developing microorganism detection apparatus according to claim 1, wherein the light wave of the light source assembly has a wavelength of 488 nm.

4. The magnetic field control-based dual color-developing microorganism detection apparatus according to claim 1, further comprising a housing (4), wherein the accommodating chamber (1) is located inside the housing (4), and the magnetic member (2) is mounted on an inner wall of the housing (4);

an observation window (41) is arranged on the shell (4), and the observation window (41) is a transparent body or an opening.

5. The magnetic field control-based dual color-development microorganism detection device according to claim 4, wherein the observation window (41) comprises a first observation window (411) and a second observation window (412), the first observation window (411) corresponds to the first detection chamber (11), and the second observation window (412) corresponds to the second detection chamber (12).

6. The magnetic field control-based dual color-developing microorganism detection device according to claim 4, further comprising a power button (6), wherein the light source component is a light source strip (5), the light source strip (5) is mounted on a port of the housing (4), the power button (6) is mounted on an outer side wall of the housing (4), and the light source strip (5) and the power button (6) are electrically connected.

7. The magnetic field control-based dual color development microorganism detection device according to claim 4, characterized in that a partition plate (42) and a waste liquid collection tank (43) are arranged in the housing (4), and two ends of the partition plate (42) are respectively the waste liquid collection tank (43) and the accommodating cavity (1);

the partition plate (42) is provided with a first through hole (421), or the outer wall of the shell (4) is provided with a second through hole, and the second through hole is communicated with the waste liquid collecting tank (43).

8. A combined detection apparatus, comprising a pretreatment vessel, a reaction vessel and the magnetic field control-based dual-color-developing microorganism detection device of any one of claims 1 to 7, wherein the pretreatment vessel is enclosed with fluorescein isothiocyanate labeled one or more receptor reagents capable of being recognized by pathogenic microorganisms, i.e., FITC-receptor reagents, the reaction vessel is enclosed with magnetic bead labeled one or more competition reagents capable of competitively binding with the receptor reagents to be detected microorganisms, i.e., magnetic bead-competition reagents, and the reaction vessel can be inserted into the magnetic field control-based dual-color-developing microorganism detection device.

Images