CN216808776U

CN216808776U - Fermentation and separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis

Info

Publication number: CN216808776U
Application number: CN202123411597.3U
Authority: CN
Inventors: 谢昕
Original assignee: Xuzhou Vocational College of Bioengineering
Current assignee: Xuzhou Vocational College of Bioengineering
Priority date: 2021-12-30
Filing date: 2021-12-30
Publication date: 2022-06-24
Anticipated expiration: 2031-12-30

Abstract

The utility model discloses a fermentation separation device for producing nattokinase by solid state fermentation of bacillus subtilis, which comprises: the fermentation assembly comprises a fermentation tank, a feeding pipe, a material taking window and a liquid inlet pipe, wherein the feeding pipe is fixedly arranged on one side of the top end of the fermentation tank; the stirring component is arranged in the fermentation tank and is used for stirring the fermentation product in the fermentation tank; the filter assembly comprises a separation plate and a sealing plate, the separation plate is fixedly installed at the bottom of the fermentation tank, a plurality of separation openings are formed in the surface of the separation plate, and the sealing plate is electrically rotated at the bottom of the separation plate through a rotating shaft. After fermentation is completed, the components in the fermentation product can be separated according to the experiment and use requirements, so that the efficiency of the whole fermentation separation is improved, and the accuracy of the internal components is improved.

Description

Fermentation and separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis

Technical Field

The utility model relates to the technical field of fungus separation, in particular to fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis.

Background

A fermenter means an apparatus for industrially carrying out microbial fermentation, and the main body thereof is generally a main cylinder made of stainless steel plate. The structure is strict and reasonable in design and processing. The steam sterilization resistant energy-saving device has the advantages of steam sterilization resistance, certain operation elasticity, minimized internal accessories, strong material and energy transfer performance, and certain adjustment to facilitate cleaning and reduce pollution, and is suitable for production of various products and energy consumption reduction.

The existing microorganism (such as bacillus subtilis) can produce nattokinase by solid state fermentation, the nattokinase is mostly placed in a fermentation tank for fermentation production, when components such as enzyme in a fermentation product need to be extracted, the fermentation product in the fermentation product needs to be taken out for separation, but the enzyme can be inactivated in the transferring process, and the components and the content in the separation product can be changed, thereby influencing the experimental result.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the following defects in the prior art, and provides fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis, wherein when components such as enzyme in a fermentation product need to be extracted, the internal fermentation product needs to be taken out and separated, but the enzyme is possibly inactivated in the transferring process, and the experimental result is influenced by the change of the components and the content in the separation product.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a fermentation separation device for producing nattokinase by solid state fermentation of bacillus subtilis comprises:

the fermentation assembly comprises a fermentation tank, a feeding pipe, a material taking window and a liquid inlet pipe, wherein the feeding pipe is fixedly arranged on one side of the top end of the fermentation tank, a liquid outlet is formed in the bottom of the fermentation tank, a material taking port is formed in one side of the fermentation tank, the material taking window is rotatably arranged on the material taking port through a rotating shaft, the liquid inlet pipe is fixedly arranged on one side of the fermentation tank, and one end of the liquid inlet pipe extends into the fermentation tank;

the stirring component is arranged in the fermentation tank and is used for stirring the fermentation product in the fermentation tank;

the filter assembly comprises a separation plate and sealing plates, the separation plate is fixedly arranged at the bottom of the fermentation tank, a plurality of separation ports are formed in the surface of the separation plate, the separation ports are in a quarter-arc shape with the circle center of the separation plate as the circle center, the radius of the separation ports gradually increases from inside to outside, the two groups of separation ports are arranged oppositely, the two sealing plates are electrically rotated at the bottom of the separation plate through a rotating shaft, and the sealing plates are in a quarter-sector shape;

the separating assembly comprises an installation tank, a centrifugal machine and a liquid bearing cup, the installation tank is fixedly installed at the bottom of the fermentation tank, the center of the circle coincides with the center of the installation tank, the centrifugal machine is fixedly installed at the bottom of the installation tank, a plurality of liquid outlets are formed in the side edge of the installation tank, and the liquid bearing cup is installed outside the installation tank through a connecting assembly.

Preferably, the stirring subassembly includes motor, dwang and stirring leaf, motor fixed mounting is at the fermentation tank top, dwang fixed connection is at the output of motor, the crisscross fixed connection of a plurality of stirring leaves on the dwang.

Preferably, install the extrusion subassembly on the dwang, the extrusion subassembly includes sliding block, clamping ring and clamp plate, the spacing groove has been seted up to the both sides that the dwang is not equipped with the stirring leaf, the clamping ring sets up inside the fermentation cylinder, the spacing slip of sliding block one end sets up in the spacing inslot portion, and is a plurality of clamp plate fixed connection is at the clamping ring inner wall.

Preferably, the clamping ring is made of elastic materials, the diameter of the outer ring is consistent with that of the fermentation tank, and the radius of the inner ring is larger than the length of the stirring blade.

Preferably, coupling assembling includes go-between and collar, collar fixed connection is in the fermentation cylinder bottom, and inside has the screw thread annular, go-between fixed connection is in holding liquid cup upper end, and threaded connection is inside the screw thread annular.

Preferably, the supporting component is installed to the fermentation cylinder bottom, the supporting component includes support column, baffle and supporting shoe, and is a plurality of support column fixed connection is in the fermentation cylinder bottom, baffle fixed connection is in the support column bottom, and inside has seted up the opening, the opening diameter is greater than the diameter of holding the liquid cup, two supporting shoe fixed connection is in the baffle bottom.

Preferably, the length of the supporting column and the supporting block is greater than that of the liquid bearing cup, and the distance between the two supporting blocks is greater than the diameter of the liquid bearing cup.

Preferably, clamp plate length is less than the clamping ring radius, and does not contact with the dwang, the directional clamping ring centre of a circle of clamp plate tip, and make by the elastic material.

Preferably, the outer side of the sealing plate is fixedly connected with a handle, and a pull groove is formed in the handle.

Compared with the prior art, the utility model has the beneficial effects that:

1. in the fermentation process, materials to be fermented and fungi to be cultured are guided into a fermentation tank through a feeding pipe, so that the materials are fermented, after the fermentation is completed, separation liquid is guided into the fermentation tank through a liquid inlet pipe, the fungi mucilage to be extracted is dissolved into the separation liquid, meanwhile, the separation speed is increased through a stirring assembly, after the dissolution is completed, an electric rotating shaft is controlled, a sealing plate is driven to rotate, so that a separation opening in the separation plate is opened, the fermented materials and the separation liquid dissolved with the fungi mucilage are separated, the separation liquid is guided into a centrifuge, through the centrifugal effect of the centrifuge, required components are guided out through the liquid opening and are guided into a liquid bearing cup, and the fermentation separation is completed. After fermentation is completed, the method can separate the components in the fermentation product according to more experimental and use requirements, so that the whole fermentation separation efficiency is improved, and the accuracy of the internal components is improved;

2. in the stirring process, fermented materials are adhered to the inner wall of the fermentation tank, so that the separation amount is reduced in the separation stage, and the final experiment effect is influenced.

Drawings

FIG. 1 is a schematic front view of a fermentation separation apparatus for producing nattokinase by solid state fermentation of Bacillus subtilis;

FIG. 2 is a schematic diagram of the back structure of a fermentation separation device for producing nattokinase by solid state fermentation of Bacillus subtilis;

FIG. 3 is a schematic sectional view of a fermentation and separation apparatus for producing nattokinase by solid-state fermentation of Bacillus subtilis according to the present invention;

FIG. 4 is a schematic front structural diagram of a stirring component of a fermentation separation device for producing nattokinase by solid state fermentation of Bacillus subtilis;

FIG. 5 is a schematic front view of a separation module of a fermentation separation device for producing nattokinase by solid state fermentation of Bacillus subtilis;

FIG. 6 is a schematic structural diagram of a filtering component of a fermentation separation device for producing nattokinase by solid state fermentation of Bacillus subtilis.

In the figure: 1 fermentation component, 11 fermentation tank, 12 feeding pipe, 13 material taking window, 14 liquid inlet pipe, 2 stirring component, 21 motor, 22 rotating rod, 23 stirring blade, 3 filtering component, 31 separating plate, 32 sealing plate, 4 separating component, 41 mounting tank, 42 centrifuge, 43 liquid bearing cup, 5 extruding component, 51 sliding block, 52 pressing ring, 53 pressing plate, 6 connecting component, 61 connecting ring, 62 mounting ring, 7 supporting component, 71 supporting column, 72 clapboard, 73 supporting block and 8 handle.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-6, a fermentation separation device for producing nattokinase by solid state fermentation of bacillus subtilis comprises:

the fermentation assembly 1 comprises a fermentation tank 11, a feeding pipe 12, a material taking window 13 and a liquid inlet pipe 14, wherein the feeding pipe 12 is fixedly arranged on one side of the top end of the fermentation tank 11, a liquid outlet is formed in the bottom of the feeding pipe, a material taking port is formed in one side of the fermentation tank 11, one side of the material taking window 13 is rotatably arranged on the material taking port through a rotating shaft, the liquid inlet pipe 14 is fixedly arranged on one side of the fermentation tank 11, and one end of the liquid inlet pipe extends into the fermentation tank 11;

the stirring assembly 2 is arranged inside the fermentation tank 11, and is used for stirring the fermentation product inside;

the filter assembly 3 comprises a separation plate 31 and sealing plates 32, the separation plate 31 is fixedly arranged at the bottom of the fermentation tank 11, the surface of the separation plate 31 is provided with a plurality of separation ports, the separation ports are in a quarter arc shape taking the circle center of the separation plate 31 as the circle center and gradually increase in radius from inside to outside, the two groups of separation ports are oppositely arranged, the two sealing plates 32 electrically rotate at the bottom of the separation plate 31 through rotating shafts, and the sealing plates 32 are in a quarter sector shape;

separating assembly 4, separating assembly 4 are including installation jar 41, centrifuge 42 and hold liquid cup 43, and installation jar 41 fixed mounting is in fermentation cylinder 11 bottoms, and the centre of a circle coincides with installation jar 41 center, and centrifuge 42 fixed mounting has seted up a plurality of liquid outlets in installation jar 41 bottom, and it installs at installation jar 41 outsidely to hold liquid cup 43 through coupling assembling 6.

In the utility model, during fermentation, materials to be fermented and fungi to be cultured are introduced into a fermentation tank 11 through a feed pipe 12, so that the materials are fermented, after the fermentation is finished, a separation liquid is introduced through a liquid inlet pipe 14, the fungi mucilage to be extracted is dissolved into the separation liquid, meanwhile, the speed is increased through separation by a stirring assembly 2, after the dissolution is finished, an electric rotating shaft is controlled to drive a sealing plate 32 to rotate, so that a separation opening on a separation plate 31 is opened, the fermented materials and the separation liquid dissolved with the fungi mucilage are separated, the separation liquid is introduced into a centrifugal machine 42, and required components are led out through the liquid opening by the centrifugal action of the centrifugal machine 42 and are introduced into a liquid receiving cup 43, so that the fermentation separation is finished. After fermentation is completed, the method can separate the components in the fermentation product according to the experiment and use requirements, so that the whole fermentation separation efficiency is improved, and the accuracy of the internal components is improved.

In the preferred technical scheme of this embodiment, the stirring assembly 2 includes a motor 21, a rotating rod 22 and stirring blades 23, the motor 21 is fixedly installed at the top of the fermentation tank 11, the rotating rod 22 is fixedly connected to the output end of the motor 21, and the stirring blades 23 are fixedly connected to the rotating rod 22 in a staggered manner, after fermentation is completed, mucus exists on a fermented product, the separated liquid is introduced through the liquid inlet pipe 14, and the fermented product is soaked in the separated liquid, but in order to improve the fermentation effect and the separation efficiency, the rotating rod 22 can be driven by the motor 21, so that the stirring blades 23 are driven to stir, in the fermentation stage, the fermentation speed can be increased, the fermentation efficiency can be improved, in the separation stage, the stirring blades 23 can accelerate the separation of the mucus and the fermented product, and the separation efficiency is improved;

the rotating rod 22 is provided with the extrusion assembly 5, the extrusion assembly 5 comprises a sliding block 51, a pressing ring 52 and pressing plates 53, two sides of the rotating rod 22, which are not provided with the stirring blades 23, are provided with limiting grooves, the pressing ring 52 is arranged inside the fermentation tank 11, one end of the sliding block 51 is arranged inside the limiting grooves in a limiting sliding manner, and the pressing plates 53 are fixedly connected to the inner wall of the pressing ring 52;

the press ring 52 is made of elastic materials, the diameter of the outer ring is consistent with that of the fermentation tank 11, the radius of the inner ring is larger than the length of the stirring blade 23, and the press ring 52 is prevented from scraping the inner wall of the fermentation tank 11;

the connecting assembly 6 comprises a connecting ring 61 and a mounting ring 62, the mounting ring 62 is fixedly connected to the bottom of the fermentation tank 11 and is internally provided with a threaded ring groove, the connecting ring 61 is fixedly connected to the upper end of the liquid bearing cup 43 and is in threaded connection with the inside of the threaded ring groove, and the connecting ring 61 is in threaded connection with the mounting ring 62, so that the mounting and fixing of the liquid bearing cup 43 are facilitated, and the separation liquid is conveniently taken;

the supporting assembly 7 is installed at the bottom of the fermentation tank 11, the supporting assembly 7 comprises supporting columns 71, partition plates 72 and supporting blocks 73, the supporting columns 71 are fixedly connected to the bottom of the fermentation tank 11, the partition plates 72 are fixedly connected to the bottoms of the supporting columns 71, openings are formed in the partition plates 72, the diameters of the openings are larger than those of the liquid bearing cups 43, the two supporting blocks 73 are fixedly connected to the bottoms of the partition plates 72, and therefore the liquid bearing cups 43 can be conveniently installed and transferred by supporting the supporting blocks 73 through the lower partition plates 72;

the lengths of the supporting column 71 and the supporting block 73 are greater than that of the liquid bearing cup 43, the distance between the two supporting blocks 73 is greater than the diameter of the liquid bearing cup 43, and in order to ensure normal use and transfer of the liquid bearing cup 43, the size and the shape between the supporting column 71 and the supporting block 73 need to be limited;

the length of the pressing plate 53 is smaller than the radius of the pressing ring 52, the pressing plate is not in contact with the rotating rod 22, the end part of the pressing plate 53 points to the circle center of the pressing ring 52 and is made of elastic materials, and the pressing plate 53 needs to separate fermentation materials adhered to the rotating rod 22 but cannot abrade the rotating rod 22;

the closing plate 32 outside fixedly connected with handle 8, the inside kerve of having seted up of handle 8, handle 8 is convenient to be operated getting material window 13 to the convenience is cleared up the fermentation thing of separating the completion.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis is characterized by comprising:

the fermentation assembly (1) comprises a fermentation tank (11), a feeding pipe (12), a material taking window (13) and a liquid inlet pipe (14), wherein the feeding pipe (12) is fixedly installed on one side of the top end of the fermentation tank (11), a liquid outlet is formed in the bottom of the feeding pipe, a material taking opening is formed in one side of the fermentation tank (11), one side of the material taking window (13) is rotatably arranged on the material taking opening through a rotating shaft, the liquid inlet pipe (14) is fixedly installed on one side of the fermentation tank (11), and one end of the liquid inlet pipe extends into the fermentation tank (11);

the stirring assembly (2) is arranged inside the fermentation tank (11) and is used for stirring the fermentation product inside the fermentation tank (2);

the filter assembly (3) comprises a separation plate (31) and sealing plates (32), the separation plate (31) is fixedly installed at the bottom of the fermentation tank (11), a plurality of separation ports are formed in the surface of the separation plate, the separation ports are in a quarter-arc shape with the circle center of the separation plate (31) as the circle center, the radius of the separation ports gradually increases from inside to outside, the two groups of separation ports are oppositely arranged, the two sealing plates (32) are electrically rotated at the bottom of the separation plate (31) through rotating shafts, and the sealing plates (32) are in a quarter-sector shape;

separation element (4), separation element (4) include installation tank (41), centrifuge (42) and hold liquid cup (43), installation tank (41) fixed mounting is in fermentation cylinder (11) bottom, and the centre of a circle coincides with installation tank (41) center, centrifuge (42) fixed mounting is in installation tank (41) bottom, a plurality of liquid outlets have been seted up to installation tank (41) side, it installs in installation tank (41) outside through coupling assembling (6) to hold liquid cup (43).

2. The fermentation separation equipment for producing nattokinase through solid state fermentation of bacillus subtilis according to claim 1, wherein the stirring component (2) comprises a motor (21), a rotating rod (22) and stirring blades (23), the motor (21) is fixedly arranged at the top of the fermentation tank (11), the rotating rod (22) is fixedly connected at the output end of the motor (21), and the stirring blades (23) are fixedly connected on the rotating rod (22) in a staggered manner.

3. The fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis according to claim 2, wherein an extrusion component (5) is arranged on the rotating rod (22), the extrusion component (5) comprises a sliding block (51), a pressing ring (52) and a pressing plate (53), a limiting groove is formed in two sides of the rotating rod (22) which are not provided with the stirring blades (23), the pressing ring (52) is arranged inside the fermentation tank (11), the sliding block (51) is arranged inside the limiting groove in a limiting sliding mode at one end, and the pressing plate (53) is fixedly connected to the inner wall of the pressing ring (52).

4. The fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis according to claim 3, wherein the pressure ring (52) is made of elastic material, the diameter of the outer ring is consistent with that of the fermentation tank (11), and the radius of the inner ring is larger than the length of the stirring blade (23).

5. The fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis according to claim 1, wherein the connecting assembly (6) comprises a connecting ring (61) and a mounting ring (62), the mounting ring (62) is fixedly connected to the bottom of the fermentation tank (11) and is internally provided with a threaded ring groove, and the connecting ring (61) is fixedly connected to the upper end of the liquid bearing cup (43) and is threaded inside the threaded ring groove.

6. The fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis according to claim 1, wherein a support assembly (7) is installed at the bottom of the fermentation tank (11), the support assembly (7) comprises support columns (71), partition plates (72) and support blocks (73), the support columns (71) are fixedly connected to the bottom of the fermentation tank (11), the partition plates (72) are fixedly connected to the bottoms of the support columns (71) and are internally provided with openings, the diameters of the openings are larger than those of the liquid bearing cups (43), and the two support blocks (73) are fixedly connected to the bottoms of the partition plates (72).

7. The fermentation separation equipment for producing nattokinase through solid state fermentation of bacillus subtilis according to claim 6, wherein the length of the supporting column (71) and the supporting block (73) is larger than that of the liquid bearing cup (43), and the distance between the two supporting blocks (73) is larger than the diameter of the liquid bearing cup (43).

8. The fermentation separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis according to claim 3, wherein the length of the pressing plate (53) is less than the radius of the pressing ring (52) and is not in contact with the rotating rod (22), and the end part of the pressing plate (53) points to the center of the pressing ring (52) and is made of elastic material.

9. The fermentation and separation equipment for producing nattokinase by solid state fermentation of bacillus subtilis according to claim 1, wherein a handle (8) is fixedly connected to the outer side of the sealing plate (32), and a pull groove is formed in the handle (8).