CN216779099U - Cord blood stem cell separation detection device - Google Patents
Cord blood stem cell separation detection device Download PDFInfo
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- CN216779099U CN216779099U CN202220471439.2U CN202220471439U CN216779099U CN 216779099 U CN216779099 U CN 216779099U CN 202220471439 U CN202220471439 U CN 202220471439U CN 216779099 U CN216779099 U CN 216779099U
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- plate
- cord blood
- fixedly connected
- stem cell
- suction pipe
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- 210000004700 fetal blood Anatomy 0.000 title claims abstract description 35
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 27
- 238000000926 separation method Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 title claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 30
- 230000006978 adaptation Effects 0.000 claims 1
- 239000006228 supernatant Substances 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 2
- 210000003954 umbilical cord Anatomy 0.000 description 4
- 239000010902 straw Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 210000003754 fetus Anatomy 0.000 description 2
- 230000002439 hemostatic effect Effects 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 210000003606 umbilical vein Anatomy 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model discloses a cord blood stem cell separation detection device, which relates to the technical field of stem cell separation and comprises a base, wherein one end of the upper surface of the base is fixedly connected with an upright post, a supporting plate is arranged above the base, the middle part of the supporting plate is movably connected with an annular plate through a bearing, and a centrifugal cylinder is inserted and connected inside the annular plate. According to the utility model, the centrifugal cylinder is arranged, the centrifugal cylinder can carry out centrifugal treatment on the cord blood to obtain stem cells, the cover plate is arranged, the sealing plate is arranged in the middle of the cover plate, the suction pipe is arranged in the middle of the sealing plate, the rubber plug is arranged on the upper surface of the sealing plate, the rubber plug acts on the suction pipe through friction force to fix the position of the suction pipe, the suction pipe is pressed downwards to move downwards, the position of the bottom end of the suction pipe can be adjusted, the position of the suction pipe can be adjusted according to the thickness of a supernatant layer of the separated cord blood, and the influence on the stem cells when the clear liquid is extracted is avoided.
Description
Technical Field
The utility model relates to the technical field of stem cell separation, in particular to a separation and detection device for cord blood stem cells.
Background
Collecting umbilical cord blood comprises collecting umbilical cord blood after birth of newborn, collecting 3-8cm umbilical cord, storing two hemostatic forceps, ligating, cutting off umbilical cord, carrying away the infant, sterilizing at mother hemostatic forceps, inserting needle into umbilical vein, and collecting umbilical cord blood. The cord blood collection is different from the traditional bone marrow collection, anesthesia is not needed, the placenta and the umbilical cord are thrown away as waste after the fetus is born, and the cord blood collection is carried out after the placenta, the umbilical cord, a mother body and the fetus are completely separated, so that no adverse effect is caused on the mother body and the child, and the cord blood collection belongs to waste utilization and waste recycling.
Need separate it earlier when detecting cord blood stem cell, adopt centrifuge to separate cord blood stem cell among the prior art, take the supernatant out through the straw after carrying out centrifugal treatment to cord blood stem cell in order to acquire pure stem cell, however, because the supernatant layer thickness after the centrifugation of different cord blood stem cell is different, hand the straw through the staff and extract the supernatant solution and cause the influence to the stem cell, moreover, current centrifugal device is inconvenient before the processing to separate the disinfection, the practicality is not enough. Therefore, it is necessary to provide an apparatus for separating and detecting umbilical cord blood stem cells to solve the above problems.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a cord blood stem cell separation and detection device to solve the above problems of the background art.
In order to achieve the purpose, the utility model provides the following technical scheme: an umbilical cord blood stem cell separation detection device comprises a base, wherein one end of the upper surface of the base is fixedly connected with an upright post, a supporting plate is arranged above the base, the middle part of the supporting plate is movably connected with an annular plate through a bearing, the interior of the annular plate is connected with a centrifugal cylinder in an inserting way, the bottom end of the outer side of the centrifugal cylinder is fixedly connected with a baffle plate with an annular structure, the lower surface of the baffle is fixedly connected with a plurality of positioning plates distributed in an annular array, the inner side of the annular plate is fixedly connected with a plurality of positioning blocks distributed in an annular array, the positioning plate is matched with the positioning block, a cover plate is arranged above the supporting plate, the middle part of the cover plate is movably connected with a sealing plate through a bearing, the centrifugal pump is characterized in that a sealing gasket is connected to the middle of the lower surface of the sealing plate in a bonding mode, the sealing gasket is matched with the centrifugal cylinder, and a suction pipe is connected in an inserting mode through a through hole formed in the middle of the sealing plate.
Preferably, the top end of the suction pipe is fixedly connected with a connecting guide pipe, and the middle part of the upper surface of the sealing plate is fixedly connected with a rubber plug with a conical structure.
Preferably, the middle part of the lower surface of the annular plate is fixedly connected with a connecting block, and a square groove is formed in the middle of the connecting block.
Preferably, the middle part of the upper surface of the base is fixedly connected with a driving motor, and an output shaft of the driving motor is fixedly connected with a square block matched with the connecting block.
Preferably, the sleeve is inlaid at one end of each of the supporting plate and the cover plate, and the upright posts are in through connection with the sleeve.
Preferably, the middle part of the outer side of the sleeve is provided with a locking bolt, one end of the locking bolt is attached to the outer side wall of the stand column, and the middle part of the outer side of the centrifugal cylinder is provided with scale marks.
The utility model has the technical effects and advantages that:
1. according to the utility model, the centrifugal cylinder is arranged, the centrifugal cylinder can carry out centrifugal treatment on the cord blood to obtain stem cells, the cover plate is arranged, the sealing plate is arranged in the middle of the cover plate, the suction pipe is arranged in the middle of the sealing plate, the rubber plug is arranged on the upper surface of the sealing plate, the rubber plug acts on the suction pipe through friction force to fix the position of the suction pipe, the suction pipe is pressed downwards to move downwards, the position of the bottom end of the suction pipe can be adjusted, the position of the suction pipe can be adjusted according to the thickness of a supernatant layer of the separated cord blood, and the influence on the stem cells when the supernatant is extracted is avoided;
2. the centrifugal cylinder is provided with the sleeve, the sleeve is fixed on the upright post through the locking bolt, the positions of the supporting plate and the cover plate can be adjusted by releasing the limit of the sleeve, the position of the centrifugal cylinder is fixed by arranging the annular plate and the baffle plate which are matched, the positions of the supporting plate and the cover plate are adjusted, and the centrifugal cylinder is pulled upwards to separate the device, so that the device is convenient to disinfect and clean.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic structural diagram of the support plate of the present invention.
FIG. 3 is a schematic view of a centrifugal cylinder according to the present invention.
FIG. 4 is a bottom view of the annular plate structure of the present invention.
In the figure: 1. a base; 2. a column; 3. a support plate; 4. an annular plate; 5. a centrifugal cylinder; 6. a baffle plate; 7. positioning a plate; 8. positioning a block; 9. a cover plate; 10. a sealing plate; 11. a gasket; 12. a straw; 13. a connecting conduit; 14. a rubber plug; 15. connecting blocks; 16. a drive motor; 17. a square block; 18. a sleeve; 19. locking the bolt; 20. scale lines are marked.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The utility model provides a cord blood stem cell separation detection device as shown in figures 1-4, which comprises a base 1, wherein one end of the upper surface of the base 1 is fixedly connected with an upright post 2, a supporting plate 3 is arranged above the base 1, the middle part of the supporting plate 3 is movably connected with an annular plate 4 through a bearing, the inside of the annular plate 4 is connected with a centrifugal cylinder 5 in an inserting way, the bottom end of the outer side of the centrifugal cylinder 5 is fixedly connected with a baffle 6 with an annular structure, the lower surface of the baffle 6 is fixedly connected with a plurality of positioning plates 7 distributed in an annular array, the inner side of the annular plate 4 is fixedly connected with a plurality of positioning blocks 8 distributed in an annular array, the positioning plates 7 are matched with the positioning blocks 8, the position between the centrifugal cylinder 5 and the annular plate 4 is fixed, a cover plate 9 is arranged above the support plate 3, and the middle part of the cover plate 9 is movably connected with a sealing plate 10 through a bearing;
specifically, the bearing is arranged to enable the sealing plate 10 to rotate, so that the rotation of the centrifugal cylinder 5 does not affect the cover plate 9, the middle of the lower surface of the sealing plate 10 is bonded with the sealing gasket 11, the sealing gasket 11 is matched with the centrifugal cylinder 5, the sealing plate 10 can seal the centrifugal cylinder 5 by matching with the sealing gasket 11, and the suction pipe 12 is inserted and connected into a through hole formed in the middle of the sealing plate 10;
more specifically, the top end of the suction tube 12 is fixedly connected with a connecting conduit 13, the middle part of the upper surface of the sealing plate 10 is fixedly connected with a rubber plug 14 with a conical structure, the suction tube 12 penetrates through the middle part of the rubber plug 14, the rubber plug 14 limits the suction tube 12 under the action of friction force, the middle part of the lower surface of the annular plate 4 is fixedly connected with a connecting block 15, a square groove is formed in the middle part of the connecting block 15, the middle part of the upper surface of the base 1 is fixedly connected with a driving motor 16, an output shaft of the driving motor 16 is fixedly connected with a square block 17 matched with the connecting block 15, and the square block 17 is matched with the connecting block 15, so that the driving motor 16 can drive the centrifugal cylinder 5 to rotate;
moreover, a sleeve 18 is inlaid at one end of the support plate 3 and one end of the cover plate 9, the upright post 2 is in through connection with the sleeve 18, a locking bolt 19 is arranged in the middle of the outer side of the sleeve 18, the position between the sleeve 18 and the upright post 2 is fixed due to the arrangement of the locking bolt 19, the position between the support plate 3 and the cover plate 9 is further fixed, one end of the locking bolt 19 is attached to the outer side wall of the upright post 2, a scale mark 20 is arranged in the middle of the outer side of the centrifuge tube 5, and the arrangement of the scale mark 20 facilitates the worker to judge the volume of each layer of the umbilical cord blood in the centrifuge tube 5.
The working principle of the utility model is as follows:
when the device is used, firstly, the cord blood is put into the centrifuge tube 5, the centrifuge tube 5 is inserted into the annular plate 4, the positioning plate 7 and the positioning block 8 are clamped with each other, the centrifuge tube 5 is pressed downwards until the lower surface of the baffle 6 is attached to the upper surface of the annular plate 4, the position of the supporting plate 3 is adjusted, the supporting plate 3 drives the annular plate 4 to move until the connecting block 15 at the bottom end of the annular plate 4 is connected with the square block 17 in an inserting manner, the locking bolt 19 is screwed down, the position between the sleeve 18 and the upright post 2 is fixed, the position of the supporting plate 3 is fixed at the moment, the position of the cover plate 9 is adjusted, the cover plate 9 drives the sealing plate 10 to move until the sealing plate 10 is attached to the upper surface of the centrifuge tube 5, the suction tube 12 is pulled upwards to separate the bottom end of the suction tube 12 from the cord blood, the locking bolt 19 is screwed down, the position of the cover plate 9 is fixed, the driving motor 16 is started, the driving motor 16 drives the centrifugal cylinder 5 to rotate, the cord blood is subjected to centrifugal treatment, the treated cord blood is layered, the position of the suction pipe 12 is adjusted, the bottom end of the suction pipe 12 is inserted into a supernatant layer of the cord blood, then the connecting conduit 13 is connected with a liquid pump, and the supernatant is pumped out, so that the separation of cord blood stem cells can be completed.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the utility model.
Claims (6)
1. The utility model provides a cord blood stem cell separation detection device, includes base (1), its characterized in that: the centrifugal centrifuge comprises a base (1), a stand column (2) is fixedly connected to one end of the upper surface of the base (1), a supporting plate (3) is arranged above the base (1), the middle of the supporting plate (3) is movably connected with an annular plate (4) through a bearing, a centrifugal cylinder (5) is connected to the inside of the annular plate (4) in an inserting manner, a baffle plate (6) with an annular structure is fixedly connected to the bottom end of the outer side of the centrifugal cylinder (5), a plurality of positioning plates (7) distributed in an annular array are fixedly connected to the lower surface of the baffle plate (6), a plurality of positioning blocks (8) distributed in the annular array are fixedly connected to the inner side of the annular plate (4), the positioning plates (7) are matched with the positioning blocks (8), a cover plate (9) is arranged above the supporting plate (3), a sealing plate (10) is movably connected to the middle of the cover plate (9) through a bearing, a sealing gasket (11) is connected to the middle of the lower surface of the sealing plate (10) in an adhering manner, the sealing gasket (11) is matched with the centrifugal cylinder (5), and a suction pipe (12) is inserted and connected in a through hole formed in the middle of the sealing plate (10).
2. The cord blood stem cell separation and detection device according to claim 1, characterized in that: the top end of the suction pipe (12) is fixedly connected with a connecting guide pipe (13), and the middle part of the upper surface of the sealing plate (10) is fixedly connected with a rubber plug (14) with a conical structure.
3. The cord blood stem cell separation and detection device according to claim 2, characterized in that: the lower surface middle part fixedly connected with connecting block (15) of annular plate (4), square groove has been seted up in the middle part of connecting block (15).
4. The cord blood stem cell separation and detection device according to claim 3, characterized in that: the upper surface middle part fixedly connected with driving motor (16) of base (1), the output shaft fixedly connected with of driving motor (16) and connecting block (15) looks adaptation square piece (17).
5. The apparatus for separating and detecting cord blood stem cells according to claim 4, wherein: the supporting plate is characterized in that one ends of the supporting plate (3) and the cover plate (9) are embedded with sleeves (18), and the upright columns (2) are in through connection with the sleeves (18).
6. The cord blood stem cell separation and detection device according to claim 5, characterized in that: the outside middle part of sleeve pipe (18) is provided with locking bolt (19), the one end of locking bolt (19) is laminated mutually with the outside wall of stand (2), the outside middle part of centrifuge bowl (5) is provided with scale mark (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220471439.2U CN216779099U (en) | 2022-03-04 | 2022-03-04 | Cord blood stem cell separation detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220471439.2U CN216779099U (en) | 2022-03-04 | 2022-03-04 | Cord blood stem cell separation detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216779099U true CN216779099U (en) | 2022-06-21 |
Family
ID=82000763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220471439.2U Expired - Fee Related CN216779099U (en) | 2022-03-04 | 2022-03-04 | Cord blood stem cell separation detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216779099U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116839990A (en) * | 2023-06-28 | 2023-10-03 | 深圳市生命力生物保健科技江苏有限公司 | Sample extraction detection device for vitamin processing |
-
2022
- 2022-03-04 CN CN202220471439.2U patent/CN216779099U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116839990A (en) * | 2023-06-28 | 2023-10-03 | 深圳市生命力生物保健科技江苏有限公司 | Sample extraction detection device for vitamin processing |
| CN116839990B (en) * | 2023-06-28 | 2024-03-19 | 深圳市生命力生物保健科技江苏有限公司 | Sample extraction detection device for vitamin processing |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220621 |