CN216778551U

CN216778551U - Elution device for polypeptide production

Info

Publication number: CN216778551U
Application number: CN202122639604.9U
Authority: CN
Inventors: 肖金根; 袁凯
Original assignee: Shanghai Ambiopharm Inc
Current assignee: Shanghai Ambiopharm Inc
Priority date: 2021-11-01
Filing date: 2021-11-01
Publication date: 2022-06-21
Anticipated expiration: 2031-11-01

Abstract

The utility model relates to the technical field of polypeptide production, in particular to an elution device for polypeptide production, which comprises a raw material tank, wherein an elution tank is arranged on the right side of the raw material tank, a liquid inlet pipe is communicated and arranged at the bottom of the right side of the raw material tank, one end of the liquid inlet pipe, which is far away from the raw material tank, penetrates through the top of the elution tank and extends into the elution tank, a control panel is arranged on the front surface of the elution tank, support plates are symmetrically arranged on the right side of the elution tank from top to bottom, a first elution liquid tank is arranged at the top of the support plate positioned on the upper side, a second elution liquid tank is arranged at the top of the support plate positioned on the lower side, the first elution liquid tank and the elution tank are communicated and connected through a first elution pipeline, in the utility model, through the elution device for polypeptide production, the elution is carried out through eluents with different concentrations, so that the polypeptide solution can achieve the good separation purpose according to the proper volume factor, the quality and efficiency of elution are improved.

Description

Elution device for polypeptide production

Technical Field

The utility model relates to the technical field of polypeptide production, in particular to an elution device for polypeptide production.

Background

Peptides are compounds of alpha-amino acids linked together by peptide bonds, which are also intermediates in the hydrolysis of proteins. Polypeptides are compounds in which alpha-amino acids are linked together by peptide bonds, and are also intermediates in the hydrolysis of proteins. The compound obtained by dehydrating and condensing two amino acid molecules is called dipeptide, and similarly, tripeptide, tetrapeptide, pentapeptide and the like are also included. Generally, a compound obtained by dehydrating and condensing three or more amino acid molecules may be called a polypeptide.

The polypeptide need elute after by protein acidolysis, and the raw materials after the elution need further adjust operations such as PH, and current elution device elution efficiency reduces, and the poor elution effect causes the diluted alkali quantity to increase, seriously reduces the elution rate of synthetic polypeptide, is unfavorable for subsequent processing production.

Therefore, it is important to design an elution apparatus for polypeptide production to overcome the above technical drawbacks and improve the overall practicability.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to an elution apparatus for polypeptide production, which solves the problems of the background art described above.

In order to achieve the purpose, the utility model provides the following technical scheme:

an elution device for polypeptide production comprises a raw material tank, wherein an elution tank is arranged on the right side of the raw material tank, a liquid inlet pipe is communicated with the bottom of the right side of the raw material tank, one end, far away from the raw material tank, of the liquid inlet pipe penetrates through the top of the elution tank and extends to the inside of the elution tank, a control panel is arranged on the front side of the elution tank, a support plate is symmetrically arranged on the right side of the elution tank from top to bottom, a first elution liquid tank is arranged at the top of the support plate on the upper side, a second elution liquid tank is arranged at the top of the support plate on the lower side, the first elution liquid tank and the elution tank are in through connection through a first elution pipeline, the second elution liquid tank and the elution tank are in through connection through a second elution pipeline, a solution discharge pipe is arranged at the bottom of the elution tank, a secondary elution pipe is connected to the left side of the solution discharge pipe, and one end, far away from the solution discharge pipe, of the secondary elution pipe is connected with the liquid inlet pipe, the right side at elution tank top and the left side of bottom all are provided with the motor, the output of motor runs through elution jar and is connected with the (mixing) shaft, two the outside of the opposite one end of (mixing) shaft all is provided with the stirring leaf that intercrossing set up, be provided with acid-base detector with the bottom of elution jar inboard.

As a preferable scheme of the present invention, valves are disposed on the outer sides of the liquid inlet pipe, the first elution pipeline, the second elution pipeline, the solution discharge pipe and the secondary elution pipe, wherein the valves are connected with the control panel through wires, and the connection mode is electrical connection.

As a preferable scheme of the utility model, the support plate and the elution tank are fixedly connected through bolts.

In a preferred embodiment of the present invention, the first eluent tank is filled with a high-concentration eluent, and the second eluent tank is filled with a low-concentration eluent.

As a preferable scheme of the utility model, the control panel is respectively connected with the motor and the acid-base detector through leads in an electrical connection mode.

As a preferable scheme of the utility model, the stirring shaft is rotationally connected with the elution tank through a bearing seat.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, through the arranged elution device for polypeptide production, the polypeptide solution can be well separated according to a proper capacity factor by eluting with eluents with different concentrations, and the elution quality and efficiency are improved.

2. According to the elution device for polypeptide production, the contact mixing area of the eluent and the polypeptide solution is increased under the stirring of the upper stirring blade and the lower stirring blade, and the two stirring blades rotate alternately, so that the stirring efficiency is higher.

3. According to the elution device for polypeptide production, provided by the utility model, the polypeptide solution can be detected by the acid-base detector before the elution is finished, the polypeptide solution can be discharged through the solution discharge pipe after the pH value is qualified, and the polypeptide solution can flow back to the liquid inlet pipe through the secondary elution pipe to be subjected to secondary elution when the pH value is unqualified, so that the elution qualification rate is improved.

Drawings

FIG. 1 is a front view of the overall structure of the present invention;

FIG. 2 is an internal view of the overall structure of the present invention;

fig. 3 is a perspective view of a partial structure of the present invention.

In the figure: 1. a raw material tank; 2. an elution tank; 201. a motor; 202. a stirring shaft; 203. stirring blades; 204. an acid-base detector; 3. a liquid inlet pipe; 4. a control panel; 5. a support plate; 6. a first eluent tank; 7. a second eluent tank; 8. a first elution conduit; 9. a second elution conduit; 10. a solution discharge pipe; 11. and (5) secondary tube elution.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.

To facilitate an understanding of the utility model, the utility model will now be described more fully with reference to the accompanying drawings. Several embodiments of the utility model are presented. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1-3, the present invention provides a technical solution:

in the embodiment, referring to fig. 1, fig. 2 and fig. 3, an elution apparatus for polypeptide production includes a material tank 1, an elution tank 2 is disposed on the right side of the material tank 1, a liquid inlet pipe 3 is disposed in communication with the bottom of the right side of the material tank 1, one end of the liquid inlet pipe 3, which is far away from the material tank 1, penetrates through the top of the elution tank 2 and extends into the elution tank 2, a control panel 4 is disposed on the front of the elution tank 2, support plates 5 are disposed on the right side of the elution tank 2 in up-and-down symmetry, a first elution tank 6 is disposed on the top of the support plate 5 on the upper side, a second elution tank 7 is disposed on the top of the support plate 5 on the lower side, the first elution tank 6 is in through connection with the elution tank 2 through a first elution pipeline 8, the second elution tank 7 is in through connection with the elution tank 2 through a second elution pipeline 9, and a solution discharge pipe 10 is disposed at the bottom of the elution tank 2, the left side of solution discharge pipe 10 is connected with secondary elution pipe 11, the one end that solution discharge pipe 10 was kept away from to secondary elution pipe 11 is connected with feed liquor pipe 3, the right side at 2 tops of elution jar and the left side of bottom all are provided with motor 201, the output of motor 201 runs through elution jar 2 and is connected with (mixing) shaft 202, the outside of two (mixing) shaft 202 one end in opposite directions all is provided with the stirring leaf 203 of intercrossing setting, be provided with acid-base detector 204 with the bottom of elution jar 2 inboards.

An embodiment, please refer to fig. 1, fig. 2 and fig. 3, an elution apparatus for polypeptide production, wherein the liquid inlet pipe 3, the first elution pipe 8, the second elution pipe 9, the solution discharge pipe 10 and the secondary elution pipe 11 are provided with valves at outer sides thereof, wherein the valves are connected with the control panel 4 through wires, and the connection mode is electrical connection, the support plate 5 is fixedly connected with the elution tank 2 through bolts, the first elution tank 6 is filled with high-concentration eluent, the second elution tank 7 is filled with low-concentration eluent, the control panel 4 is respectively connected with the motor 201 and the acid-base detector 204 through wires, and the connection mode is electrical connection, and the stirring shaft 202 is rotatably connected with the elution tank 2 through a bearing seat.

The working process of the utility model is as follows: when the device is used, the valve, the motor 201 and the acid-base detector 204 are started through the control panel 4, the polypeptide solution in the raw material tank 1 enters the elution tank 2 through the liquid inlet pipe 3, then the motor 201 drives the stirring shaft 202 and the stirring blades 203 to rotate alternately, and then the polypeptide solution is introduced into high-concentration and low-concentration eluents in the first elution tank 6 and the second elution tank 7 through the first elution pipeline 8 and the second elution pipeline 9 at different times, the polypeptide solution can be well separated according to a proper capacity factor by eluting through eluents with different concentrations, the elution quality and efficiency are improved, the contact mixing area of the eluents and the polypeptide solution is increased under the stirring of the upper and lower groups of stirring blades 203, the polypeptide solution can be detected by the acid-base detector 204 before the elution is finished, and the polypeptide solution can be discharged through the solution discharge pipe 10 after the acid-base value is qualified, and unqualified detection can be performed through the secondary elution pipe 11 and then flows back to the liquid inlet pipe 3 for secondary elution, so that the elution qualification rate is improved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an elution device is used in polypeptide production, includes head tank (1), its characterized in that: the right side of the raw material tank (1) is provided with an elution tank (2), the bottom on the right side of the raw material tank (1) is communicated with a liquid inlet pipe (3), one end, far away from the raw material tank (1), of the liquid inlet pipe (3) penetrates through the top of the elution tank (2) and extends to the inside of the elution tank (2), the front of the elution tank (2) is provided with a control panel (4), the right side of the elution tank (2) is provided with a support plate (5) in an up-down symmetrical manner, the top of the support plate (5) positioned at the upside is provided with a first elution liquid tank (6), the top of the support plate (5) positioned at the downside is provided with a second elution liquid tank (7), the first elution liquid tank (6) is in through connection with the elution tank (2) through a first elution pipeline (8), and the second elution liquid tank (7) is in through connection with the elution tank (2) through a second elution pipeline (9), the bottom of elution jar (2) is provided with solution discharge pipe (10), the left side of solution discharge pipe (10) is connected with secondary elution pipe (11), the one end that solution discharge pipe (10) were kept away from in secondary elution pipe (11) is connected with feed liquor pipe (3), the right side at elution jar (2) top and the left side of bottom all are provided with motor (201), the output of motor (201) runs through elution jar (2) and is connected with (mixing) shaft (202), two the outside of (mixing) shaft (202) one end in opposite directions all is provided with stirring leaf (203) that intercrossing set up, be provided with acid-base detector (204) with the bottom of elution jar (2) inboard.

2. The elution device for polypeptide production according to claim 1, wherein: the outside of feed liquor pipe (3), first elution pipeline (8), second elution pipeline (9), solution discharge pipe (10) and secondary elution pipe (11) all is provided with the valve, wherein is connected through the wire between valve and control panel (4), and the mode of connecting is electric connection.

3. The elution apparatus for polypeptide production according to claim 2, wherein: the supporting plate (5) is fixedly connected with the elution tank (2) through bolts.

4. The elution apparatus for polypeptide production according to claim 3, wherein: the first eluent tank (6) is filled with high-concentration eluent, and the second eluent tank (7) is filled with low-concentration eluent.

5. The elution apparatus for polypeptide production according to claim 4, wherein: the control panel (4) is respectively connected with the motor (201) and the acid-base detector (204) through wires, and the connection mode is electric connection.

6. The elution apparatus for polypeptide production according to claim 5, wherein: the stirring shaft (202) is rotationally connected with the elution tank (2) through a bearing seat.