CN216764939U - Enzyme-assisted extraction device for enteromorpha polysaccharide - Google Patents

Enzyme-assisted extraction device for enteromorpha polysaccharide Download PDF

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Publication number
CN216764939U
CN216764939U CN202220510327.3U CN202220510327U CN216764939U CN 216764939 U CN216764939 U CN 216764939U CN 202220510327 U CN202220510327 U CN 202220510327U CN 216764939 U CN216764939 U CN 216764939U
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China
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cavity
extraction
lifting seat
enteromorpha
enzyme
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CN202220510327.3U
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Chinese (zh)
Inventor
柴勤力
孙秋艳
宋璐
付城深
宋艺琳
冯梓豪
李耀星
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Abstract

The utility model provides an enzyme-assisted extraction device of enteromorpha polysaccharide, which relates to the technical field of polysaccharide extraction devices and comprises an outer shell, wherein an extraction cavity, a filter cavity and an installation cavity are arranged in the outer shell, a first lifting seat is slidably arranged in the extraction cavity, a stirring shaft in transmission connection with a first driving mechanism is rotatably arranged on the first lifting seat, a first stirring blade and a second stirring blade are fixed on the stirring shaft, a second lifting seat and a second driving mechanism are slidably arranged in the installation cavity, a feeding pipe is fixed at the bottom of the first lifting seat, a sliding seat is slidably arranged in the filter cavity, an inlet and outlet opening is formed in one side of the sliding seat, a sealing door is arranged at the inlet and outlet opening, a beaker and a buchner funnel are placed on the sliding seat and communicated with a vacuum pump through a rubber hose, the utility model realizes high-efficiency extraction and filtration of enteromorpha solution, and solves the problem of low extraction efficiency caused by non-uniform mixing of enzyme and the enteromorpha solution, shortens the extraction time of the enteromorpha polysaccharide.

Description

Enzyme-assisted extraction device for enteromorpha polysaccharide
Technical Field
The utility model relates to the technical field of polysaccharide extraction devices, in particular to an enzyme-assisted method extraction device for enteromorpha polysaccharide.
Background
Enteromorpha polysaccharide is one of main active ingredients of Enteromorpha, and has various biological activities of immunoregulation, oxidation resistance, tumor resistance, blood fat reduction and the like. In recent years, along with environmental deterioration and climate change, a large amount of enteromorpha is bred in coastal areas of China every year, the survival of marine organisms and the safety of marine transportation are seriously influenced, so that a large amount of manpower and material resources are needed to be spent for fishing and clearing the enteromorpha, and a large amount of research on enteromorpha polysaccharide is needed in order to deeply develop the valuable resource.
The main preparation processes of the enteromorpha polysaccharide comprise three processes: compared with the solution extraction method, the enzyme-assisted extraction method reduces the requirement on temperature in the preparation process and can obviously improve the extraction rate of the enteromorpha polysaccharide. However, the existing extraction device has low stirring efficiency on the enteromorpha solution, so that the enzyme and the enteromorpha solution are not uniformly and fully mixed, the extraction efficiency of the enteromorpha polysaccharide is low, and the enteromorpha polysaccharide raw liquid does not have the function of filtering the extracted enteromorpha polysaccharide raw liquid.
Therefore, an enzyme-assisted extraction device for enteromorpha polysaccharide capable of solving the problems is needed.
SUMMERY OF THE UTILITY MODEL
The utility model provides an enzyme-assisted extraction device for enteromorpha polysaccharide, which realizes high-efficiency extraction and filtration of an enteromorpha solution, solves the problem of low extraction efficiency caused by non-uniform mixing of enzyme and the enteromorpha solution, and shortens the extraction time of the enteromorpha polysaccharide.
The technical scheme of the utility model is realized as follows:
the enzyme-assisted extraction device for enteromorpha polysaccharide comprises an outer shell, wherein an extraction cavity and a filter cavity are arranged inside the outer shell, a feed hopper and a water inlet pipe are mounted on the side wall of the outer shell, and the feed hopper and the water inlet pipe are both communicated with the extraction cavity;
the extraction cavity is internally provided with a first lifting seat, the first lifting seat is arranged in the extraction cavity in a vertical sliding manner, a vertically arranged stirring shaft is rotatably arranged on the first lifting seat, the stirring shaft is in transmission connection with a first driving mechanism for driving the stirring shaft to rotate, a plurality of first stirring blades are fixed on the side wall of the stirring shaft, a plurality of second stirring blades are arranged at the lower end of the stirring shaft, the shape of the second stirring blades is matched with the bottom wall of the extraction cavity, an installation cavity is arranged above the extraction cavity, a second lifting seat is vertically and slidably arranged in the installation cavity, the second lifting seat is fixedly connected with the first lifting seat through a connecting cylinder body, a second driving mechanism for driving the second lifting seat to slide is arranged in the installation cavity, and a communicating pipe is arranged at the bottom of the extraction cavity, a solution pump is arranged at the feed end of the communicating pipe;
a feeding pipe is fixed at the bottom of the first lifting seat and is arranged annularly, a feeding pipe is arranged in the connecting cylinder, one end of the feeding pipe is communicated with the feeding pipe, the other end of the feeding pipe extends out of the outer shell, and a plurality of feeding ports for introducing cellulase into the extraction cavity are arranged at the bottom of the feeding pipe;
the filter chamber is internally provided with a sliding seat along the horizontal direction in a sliding manner, one side of the sliding seat is provided with an inlet and outlet opening, a sealing door is installed at the inlet and outlet opening, a beaker is placed on the sliding seat, a Buchner funnel is installed at the opening at the top of the beaker, the discharge end of the communicating pipe is positioned above the Buchner funnel, and the Buchner funnel is communicated with a vacuum pump through a rubber hose.
As a preferred technical scheme, a plurality of electric heating pipes are installed on the side wall of the extraction cavity.
As a preferred technical scheme, a plurality of extension springs are installed between the bottom of the second lifting seat and the bottom wall of the installation cavity.
As a preferable technical scheme, the first driving mechanism comprises a first driving motor arranged inside the connecting cylinder, a motor shell of the first driving motor is fixed at the top of the first lifting seat, and a motor shaft of the first driving motor is in transmission connection with the stirring shaft.
As a preferred technical scheme, the second driving mechanism includes that the rotation axis in the installation cavity is installed in the rotation along the horizontal direction, be equipped with a pair of opening on the lateral wall of connecting the barrel, the rotation axis runs through two the opening connect the barrel, the rotation axis transmission is connected with second driving motor, the equal fixed mounting in both ends of rotation axis has eccentric cam, each eccentric cam's lateral wall all support by in the bottom of second lift seat.
As a preferred technical scheme, a stop block is fixed on the bottom wall of the filter cavity, one end of the sliding seat abuts against the stop block, and the other end of the sliding seat abuts against the inner wall of the sealing door.
By adopting the technical scheme, the utility model has the beneficial effects that:
according to the utility model, the enteromorpha polysaccharide is extracted in the extraction cavity, the enteromorpha polysaccharide stock solution obtained after extraction can be introduced into the filtering cavity for filtering, impurities in the enteromorpha polysaccharide are filtered by utilizing the vacuum filtration principle to obtain a filtrate, a structure for filtering the enteromorpha polysaccharide stock solution is additionally arranged on the extraction device, and an experimenter does not need to use other filtering devices to filter the obtained enteromorpha polysaccharide stock solution, so that the operation steps of the experimenter are simplified, and the extraction efficiency of the enteromorpha polysaccharide is improved.
Because the first lifting seat is slidably mounted in the extraction cavity, the stirring shaft is mounted on the first lifting seat, the first stirring blade and the second stirring blade are driven by the stirring shaft to rotate, the first lifting seat is connected with the second driving mechanism, in the utility model, the first driving mechanism is used for driving the stirring shaft to rotate, the stirring shaft drives the first stirring blade and the second stirring blade to rotate, the rotating first stirring blade and the rotating second stirring blade can stir the enteromorpha solution in the horizontal direction, the second driving mechanism is used for driving the first lifting seat, the stirring shaft, the first stirring blade and the second stirring blade to move up and down, the first stirring blade and the second stirring blade which move up and down can stir the solution in the vertical direction, the enteromorpha powder can be quickly mixed with a water body to form the enteromorpha solution, and the enteromorpha solution can be quickly and fully mixed with cellulase, therefore, the problem of low extraction efficiency caused by non-uniform mixing of the enzyme and the enteromorpha solution is effectively solved, the extraction time of the enteromorpha polysaccharide is shortened, and the efficient extraction of the enteromorpha polysaccharide is realized.
The feeding pipe is fixed at the bottom of the first lifting seat and is arranged annularly, so that the efficiency and uniformity of mixing of cellulase and enteromorpha solution can be improved, and the extraction efficiency of enteromorpha polysaccharide is ensured.
According to the utility model, the sliding seat is arranged in the filtering cavity in a sliding manner along the horizontal direction, the beaker and the Buchner funnel are arranged on the sliding seat, an inlet and outlet opening is formed in one side of the sliding seat, the sealing door is arranged at the inlet and outlet opening, vacuum filtration is carried out on enteromorpha polysaccharide stock solution by using the Buchner funnel, filtered liquid obtained by the vacuum filtration can enter the beaker, filtered impurities are intercepted in the Buchner funnel, after the filtering work is completed, an experimenter can open the sealing door, pull the sliding seat out of the shell through the inlet and outlet opening, pull the rubber hose out of the Buchner funnel, and take the beaker and the Buchner funnel out for subsequent treatment, so that the use is very simple and convenient.
Because the side wall of the extraction cavity is provided with the plurality of electric heating pipes, in the utility model, the electric heating pipes are used for heating the enteromorpha solution in the extraction cavity so as to keep the temperature of the enteromorpha solution in the temperature range required by extraction, thereby ensuring the extraction efficiency of enteromorpha polysaccharide.
Because a plurality of extension springs are arranged between the bottom of the second lifting seat and the bottom wall of the mounting cavity, the extension springs play a role in shock absorption, and large vibration of the second lifting seat and the first lifting seat in the moving process is avoided.
Because the bottom wall of the filter cavity is fixed with the stop block, in the utility model, two ends of the sliding seat respectively abut against the stop block and the inner wall of the sealing door, and the stop block and the sealing door are utilized to limit the sliding seat, thereby avoiding the situation that the sliding seat shakes to influence the filtering work.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
wherein: 1. an outer housing; 2. an extraction chamber; 3. a filter chamber; 4. a feed hopper; 5. a water inlet pipe; 6. a first lifting seat; 7. a stirring shaft; 8. a first stirring blade; 9. a second stirring blade; 10. a mounting cavity; 11. a second lifting seat; 12. connecting the cylinder body; 13. a communicating pipe; 14. a solution pump; 15. a feeding pipe; 16. a feed pipe; 17. a feeding port; 18. a sliding seat; 19. an access opening; 20. a sealing door; 21. a beaker; 22. a Buchner funnel; 23. a rubber hose; 24. a vacuum pump; 25. an electric heating tube; 26. an extension spring; 27. a first drive motor; 28. a rotating shaft; 29. an opening; 30. a second drive motor; 31. an eccentric cam.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figure 1, enzyme-assisted extraction device of enteromorpha polysaccharide, including shell body 1, shell body 1 is inside to be equipped with and to draw chamber 2 and filter chamber 3, installs feeder hopper 4 and inlet tube 5 on the lateral wall of shell body 1, and feeder hopper 4 and inlet tube 5 all are linked together with drawing chamber 2.
The enteromorpha prolifera extracting device is characterized in that a first lifting seat 6 is arranged in the extracting cavity 2, the first lifting seat 6 is vertically and slidably installed in the extracting cavity 2, a vertically-arranged stirring shaft 7 is rotatably installed on the first lifting seat 6, the stirring shaft 7 is coaxially arranged with the extracting cavity 2, the stirring shaft 7 is in transmission connection with a first driving mechanism for driving the stirring shaft 7 to rotate, a plurality of first stirring blades 8 are fixed on the side wall of the stirring shaft 7, a plurality of second stirring blades 9 are installed at the lower end of the stirring shaft 7, and the shape of the second stirring blades 9 is matched with the bottom wall of the extracting cavity 2; draw the top in chamber 2 and be provided with installation cavity 10, there is second lift seat 11 along vertical slidable mounting in installation cavity 10, second lift seat 11 is through connecting barrel 12 and first lift seat 6 fixed connection, install one in the installation cavity 10 and be used for driving the gliding second actuating mechanism of second lift seat 11, draw the bottom in chamber 2 and install a communicating pipe 13, a solution pump 14 is installed to the feed end of communicating pipe 13, in this embodiment, solution pump 14 can adopt the solution pump of the MVKD 4002 model of WALTON ALSO.
In the utility model, the first driving mechanism is used for driving the first stirring blade 8 and the second stirring blade 9 to rotate so as to stir the enteromorpha solution in the horizontal direction, and the second driving mechanism is used for driving the first stirring blade 8 and the second stirring blade 9 to move up and down so as to stir the enteromorpha solution in the vertical direction, so that enteromorpha powder can be quickly mixed with a water body to form the enteromorpha solution, and the enteromorpha solution can be quickly and fully mixed with cellulase, thereby effectively solving the problem of low extraction efficiency caused by uneven mixing of the enzyme and the enteromorpha solution, shortening the extraction time of enteromorpha polysaccharide and realizing the efficient extraction of enteromorpha polysaccharide.
The bottom of first seat 6 is fixed with a feeding pipe 15 that is the annular setting, connects barrel 12 internally mounted and has a feeding pipe 16, and the one end and the feeding pipe 15 of feeding pipe 16 are linked together, and the shell body 1 is stretched out to the other end of feeding pipe 16, and a plurality of is installed to the bottom of feeding pipe 15 and is used for drawing the dog-house 17 that the chamber 2 let in the cellulase.
A sliding seat 18 is installed in the filter chamber 3 in a sliding manner along the horizontal direction, an inlet and outlet opening 19 is formed in one side of the sliding seat 18, a sealing door 20 is installed at the inlet and outlet opening 19, a beaker 21 is placed on the sliding seat 18, a buchner funnel 22 is installed at the opening at the top of the beaker 21, the discharge end of the communication pipe 13 is located above the buchner funnel 22, the buchner funnel 22 is communicated with a vacuum pump 24 through a rubber hose 23, and one end of the rubber hose 23 can be installed on the buchner funnel 22 in a pulling and inserting manner.
Wherein, a plurality of electric heating pipes 25 are arranged on the side wall of the extraction cavity 2.
A plurality of extension springs 26 are arranged between the bottom of the second lifting seat 11 and the bottom wall of the mounting cavity 10.
Moreover, the first driving mechanism comprises a first driving motor 27 arranged inside the connecting cylinder 12, a motor housing of the first driving motor 27 is fixed on the top of the first lifting seat 6, and a motor shaft of the first driving motor 27 is in transmission connection with the stirring shaft 7.
In addition, the second driving mechanism includes a rotating shaft 28 rotatably installed in the installation cavity 10 along the horizontal direction, a pair of openings 29 is provided on the side wall of the connection cylinder 12, the rotating shaft 28 penetrates through the connection cylinder 12 through the two openings 29, the rotating shaft 28 is connected with a second driving motor 30 in a transmission manner, eccentric cams 31 are fixedly installed at both ends of the rotating shaft 28, and the side wall of each eccentric cam 31 abuts against the bottom of the second lifting seat 11.
The method of the utility model is used as follows:
firstly, starting a first driving motor 27 and a second driving motor 30, wherein the first driving motor 27 drives a stirring shaft 7, a first stirring blade 8 and a second stirring blade 9 to rotate rapidly, the second driving motor 30 drives a rotating shaft 28 and an eccentric cam 31 to rotate, and under the pushing action of the eccentric cam 31 and the action of gravity, a first lifting seat 6 and a second lifting seat 11 reciprocate along the vertical direction, so that the stirring shaft 7, the first stirring blade 8 and the second stirring blade 9 are driven to move in the vertical direction;
secondly, introducing a proper amount of water into the extraction cavity 2 through the water inlet pipe 5, pouring enteromorpha powder into the extraction cavity 2 through the feed hopper 4, stirring and mixing the water and the enteromorpha powder by the first stirring blade 8 and the second stirring blade 9 to form an enteromorpha solution, and then starting the electric heating pipe 25 to heat the enteromorpha solution so as to maintain the temperature of the enteromorpha solution within the temperature range required by the extraction work;
thirdly, after the water body is mixed with the enteromorpha powder, cellulase is fed into the extraction cavity 2 through the feeding pipe 16, the feeding pipe 15 and the feeding port 17, and the cellulase is fully mixed with the enteromorpha solution under the mixing action of the first stirring blade 8 and the second stirring blade 9, meanwhile, the cellulase can rapidly disintegrate cell walls of enteromorpha cells, so that polysaccharide components in the enteromorpha cell vacuole rapidly flow out, and an enteromorpha polysaccharide stock solution is formed in the extraction cavity 2;
fourthly, after the extraction work is finished, starting the solution pump 14 and the vacuum pump 24, enabling the enteromorpha polysaccharide stock solution in the extraction cavity 2 to enter the communicating pipe 13 under the action of the solution pump 14, pouring the enteromorpha polysaccharide stock solution into the Buchner funnel 22 through the communicating pipe 13, and forming negative pressure inside the Buchner funnel 22 under the action of the vacuum pump 24, so that the enteromorpha polysaccharide stock solution can be filtered, the obtained filtrate can drip into the beaker 21, and solid impurities in the enteromorpha polysaccharide stock solution are left in the Buchner funnel 22;
and fifthly, after the filtering work is finished, the experimenter opens the sealing door 20, pulls the sliding seat 18 out of the outer shell 1, then pulls out the rubber hose 23 connected with the vacuum pump 24, and then the experimenter can take out the beaker 21 and the Buchner funnel 22 to perform subsequent treatment on the obtained filtrate.
In conclusion, the enzyme-assisted extraction device for enteromorpha polysaccharide provided by the utility model realizes efficient extraction and filtration of enteromorpha solution, solves the problem of low extraction efficiency caused by uneven mixing of enzyme and enteromorpha solution, and shortens the extraction time of enteromorpha polysaccharide.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (6)

1. The enzyme-assisted extraction device for enteromorpha polysaccharide is characterized by comprising an outer shell, wherein an extraction cavity and a filter cavity are arranged inside the outer shell, a feed hopper and a water inlet pipe are mounted on the side wall of the outer shell, and the feed hopper and the water inlet pipe are communicated with the extraction cavity;
the extraction cavity is internally provided with a first lifting seat, the first lifting seat is arranged in the extraction cavity in a vertical sliding manner, a vertically arranged stirring shaft is rotatably arranged on the first lifting seat, the stirring shaft is in transmission connection with a first driving mechanism for driving the stirring shaft to rotate, a plurality of first stirring blades are fixed on the side wall of the stirring shaft, a plurality of second stirring blades are arranged at the lower end of the stirring shaft, the shape of the second stirring blades is matched with the bottom wall of the extraction cavity, an installation cavity is arranged above the extraction cavity, a second lifting seat is vertically and slidably arranged in the installation cavity, the second lifting seat is fixedly connected with the first lifting seat through a connecting cylinder body, a second driving mechanism for driving the second lifting seat to slide is arranged in the installation cavity, and a communicating pipe is arranged at the bottom of the extraction cavity, a solution pump is arranged at the feed end of the communicating pipe;
a feeding pipe is fixed at the bottom of the first lifting seat and is arranged annularly, a feeding pipe is arranged in the connecting cylinder, one end of the feeding pipe is communicated with the feeding pipe, the other end of the feeding pipe extends out of the outer shell, and a plurality of feeding ports for introducing cellulase into the extraction cavity are arranged at the bottom of the feeding pipe;
the filter chamber is internally provided with a sliding seat along the horizontal direction in a sliding manner, one side of the sliding seat is provided with an inlet and outlet opening, a sealing door is installed at the inlet and outlet opening, a beaker is placed on the sliding seat, a Buchner funnel is installed at the opening at the top of the beaker, the discharge end of the communicating pipe is positioned above the Buchner funnel, and the Buchner funnel is communicated with a vacuum pump through a rubber hose.
2. The enzyme-assisted extraction device of enteromorpha polysaccharide according to claim 1, wherein a plurality of electric heating pipes are installed on the side wall of the extraction cavity.
3. The enzyme-assisted extraction device of enteromorpha polysaccharide according to claim 2, wherein a plurality of extension springs are arranged between the bottom of the second lifting seat and the bottom wall of the installation cavity.
4. The enzyme-assisted extraction device of enteromorpha polysaccharide according to claim 3, wherein the first driving mechanism comprises a first driving motor arranged inside the connection cylinder, a motor shell of the first driving motor is fixed on the top of the first lifting seat, and a motor shaft of the first driving motor is in transmission connection with the stirring shaft.
5. The enzyme-assisted extraction device of enteromorpha polysaccharide according to claim 4, wherein the second driving mechanism comprises a rotating shaft rotatably mounted in the mounting cavity along the horizontal direction, a pair of openings are formed in the side wall of the connecting cylinder, the rotating shaft penetrates through the connecting cylinder through the two openings, the rotating shaft is connected with a second driving motor in a transmission manner, eccentric cams are fixedly mounted at two ends of the rotating shaft, and the side wall of each eccentric cam abuts against the bottom of the second lifting seat.
6. The enzyme-assisted extraction device of enteromorpha polysaccharide according to claim 5, wherein a stop block is fixed on the bottom wall of the filter chamber, one end of the sliding seat abuts against the stop block, and the other end of the sliding seat abuts against the inner wall of the sealing door.
CN202220510327.3U 2022-03-10 2022-03-10 Enzyme-assisted extraction device for enteromorpha polysaccharide Expired - Fee Related CN216764939U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202220510327.3U CN216764939U (en) 2022-03-10 2022-03-10 Enzyme-assisted extraction device for enteromorpha polysaccharide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202220510327.3U CN216764939U (en) 2022-03-10 2022-03-10 Enzyme-assisted extraction device for enteromorpha polysaccharide

Publications (1)

Publication Number Publication Date
CN216764939U true CN216764939U (en) 2022-06-17

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ID=81958596

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202220510327.3U Expired - Fee Related CN216764939U (en) 2022-03-10 2022-03-10 Enzyme-assisted extraction device for enteromorpha polysaccharide

Country Status (1)

Country Link
CN (1) CN216764939U (en)

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Granted publication date: 20220617