CN216753366U

CN216753366U - Closed ultra-low temperature freezing storage device

Info

Publication number: CN216753366U
Application number: CN202122969378.0U
Authority: CN
Inventors: 王传杰; 李涛
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-11-30
Filing date: 2021-11-30
Publication date: 2022-06-17
Anticipated expiration: 2031-11-30

Abstract

The utility model discloses a closed ultralow temperature frozen storage device, which comprises a sheath, a carrying rod inserted into the sheath, and a locking member for locking the sheath and the carrying rod, wherein the rod body of the carrying rod is completely wrapped in the sheath, the locking member and the sheath completely seal the rod body of the carrying rod to prevent liquid nitrogen from flowing into the sheath to pollute embryos on slide glass, and meanwhile, when the carrying rod is inserted into the sheath, the locking sleeve is locked with the sheath through threads, a first sealing ring and a second sealing ring are surrounded by the sheath, the carrying rod and the locking sleeve, and the second sealing ring is positioned on a conical surface on the inner wall of the insertion end of the sheath.

Description

Closed ultra-low temperature freezing storage device

Technical Field

The utility model relates to the technical field of assisted reproduction, in particular to a closed ultralow-temperature freezing and storing device.

Background

Assisted Reproductive Technology (ART) refers to a technique for gestation of infertile couples by medical aids, in which cryopreservation and resuscitation of gametes (sperm and ovum) and embryos is an important derivative technique.

The embryo quick-freezing storage carrier generally adopted by the current domestic and overseas reproductive medicine centers is divided into an open type and a closed type. The embryo can be directly contacted with the liquid nitrogen by using the open carrier, the cooling rate of the form is the fastest, and the freezing recovery effect is very ideal, but the form can cause potential viruses, microorganisms or chemical substances in the liquid nitrogen to influence the embryo, and even bring great potential hazards to the safety of offspring. Open freezing methods (e.g., cryo, Cryoleaf, Strawtop, etc.) have plagued assisted reproductive practitioners because of the potential contamination and cross-infection risks associated with embryos coming into direct contact with liquid nitrogen, due to their simplicity of operation and rapid cooling rates (above 20,000 ℃/min). Although closed vehicles are inferior to open vehicles in terms of cooling rate and freeze-thaw performance, they are increasingly receiving attention from various ART laboratories due to their relatively safe nature. In order to avoid pollution, closed type straw straws (CBS) and Rapid-i and other closed type vitrification freezing methods are also reported for freezing embryos clinically, but the operation is complex, and in the sealing process, partial liquid nitrogen is heated and gasified by ultrasonic waves or heat generated in the heat sealing process, so that the survival rate of the embryos or gametes is influenced, so that the method cannot be widely popularized so far, and is accepted by most IVF workers. The sealing body and the container are sealed at the same temperature and then placed in liquid nitrogen or other refrigerants, and the sealing body and the container are not suitable for the freezing requirements of embryos and gametes. Patent CN201921401744.9 is a closed loading device for embryo rapid freezing, the utility model effectively solves the technical problem of unreliable sealing of closed carrier in the prior art by arranging an accommodating cavity capable of completely accommodating a carrying rod in an outer envelope and sealing an opening of the accommodating cavity by sealant. However, in the technology, the opening of the accommodating cavity is sealed by the sealant, so that the opening is difficult, the frozen embryo on the carrier is easy to damage when the accommodating cavity is opened by using great force, and in addition, the embryo is easy to be polluted by the sealant in the opening process.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problems, the utility model provides a closed type ultralow temperature freezing storage device, which is used for solving the problems that a carrying rod and a carrying sleeve of a closed type carrier in the prior art are difficult to open and easily pollute embryos. The technical scheme adopted by the utility model for realizing the purpose is as follows:

a closed ultra-low temperature freezing and storing device comprises a sheath, a carrying rod inserted into the sheath, and a locking piece used for locking the sheath and the carrying rod;

the insertion end of the sheath is provided with a first opening, a first cavity for accommodating the carrier rod to be inserted is formed in the insertion end of the sheath, the first opening is communicated with the first cavity, and the inner wall of the insertion end is a conical surface;

the locking piece comprises a locking sleeve, a first sealing ring and a second sealing ring, the inner lower wall of the locking sleeve is in threaded connection with the insertion end of the sheath, and an extrusion surface in extrusion fit with the top surface of the first sealing ring is arranged in the locking sleeve; the first sealing ring is communicated up and down, the lower outer peripheral surface of the first sealing ring is an inverted conical surface, the upper inner peripheral surface of the first sealing ring is provided with an annular groove, and the lower inner peripheral surface is a first clamping surface for clamping the carrier rod; the second sealing ring is an inverted frustum body which is communicated up and down, the outer peripheral surface of the second sealing ring is an inverted conical surface, the upper inner peripheral surface of the second sealing ring is a chamfered surface, and the lower inner peripheral surface of the second sealing ring is a second clamping surface for clamping the carrier rod; the lower outer peripheral surface of the first sealing ring is in extrusion fit with the chamfered surface of the upper inner peripheral surface of the second sealing ring; the carrying rod is inserted into the sheath, the locking sleeve is locked with the sheath through threads, the first sealing ring and the second sealing ring are surrounded by the sheath, the carrying rod and the locking sleeve, and the second sealing ring is positioned on the conical surface of the inner wall of the insertion end of the sheath.

Furthermore, the carrying rod comprises a handle part, a rod body and a slide glass which is arranged at the front end of the rod body and used for loading embryos, and the rear end of the carrying rod is provided with the handle part. Handle portion can be that the cavity is square, can place electronic tags RFID on the one hand, and on the other hand makes things convenient for handheld pole loading embryo that carries, simultaneously, opens this closed super low temperature freezing storage device as needs, can hand the handle portion that carries the pole, the fixed hexagonal that unfreezes, the pole is carried in the rotation gently, the freezing hexagonal of sheath and the thin wall between the hexagonal that unfreezes mouthful fracture, the hexagonal that unfreezes of sheath reaches following part and all drops, can take the pole that carries this moment and remove and unfreeze.

Furthermore, the electronic tag RFID is further included, a second opening is arranged at the top end of the handle part, a second cavity for accommodating the electronic tag RFID is formed in the handle part, and the second opening leads to the second cavity. The electronic tag RFID is used for automatic identification of the sample.

Furthermore, the RFID tag also comprises a plug used for preventing the RFID tag from falling off, and the plug plugs the second opening at the top end of the handle part. The plug can block the second opening on one hand and prevent falling of electronic tag RFID, and on the other hand can fix electronic tag RFID in the cavity of handle portion.

Furthermore, the platform also comprises a boss used for preventing the carrying rod from rolling on the workbench, and the boss is arranged between the rod body and the handle part. The boss may be a cuboid.

Still further, still include the label portion for labelling, the label portion sets up between boss and handle portion.

Furthermore, the periphery of the locking sleeve is provided with a hexagonal column structure which is convenient for screwing the locking sleeve.

Furthermore, the sheath also comprises a freezing hexagonal head used for clamping the sheath in the using process, and the freezing hexagonal head is arranged below the thread of the insertion end of the sheath.

Furthermore, the sheath also comprises a thawing hexagonal which is arranged below the freezing hexagonal, and the joint of the thawing hexagonal and the freezing hexagonal is a thin-wall thawing opening which is convenient to break when the embryo needs to be taken out.

Furthermore, the top surface of the first sealing ring is an inclined plane with a high inside and a low outside. The inner extrusion surface of the sheath is convenient to extrude with the top surface of the first sealing ring, so that the annular groove in the first sealing ring is deformed, and the load rod is clamped more firmly.

Terms such as "inner", "outer", "front", "back", and the like, used herein to denote relative spatial positions, are used for ease of description to describe one feature's relationship to another feature as illustrated in the figures. It will be understood that the spatially relative positional terms may be intended to encompass different orientations than those shown in the figures depending on the product presentation position and should not be construed as limiting the claims.

Compared with the prior art, the utility model has the following beneficial effects:

1. the utility model embodiment of the closed type ultralow temperature freezing and storing device comprises a sheath, a carrying rod inserted into the sheath and a locking member for locking the sheath and the carrying rod, wherein the rod body of the carrying rod is completely wrapped in the sheath, the rod body of the carrying rod is completely sealed by the locking member and the sheath, liquid nitrogen is prevented from flowing into the sheath to pollute embryos on a slide glass, meanwhile, when the carrying rod is inserted into the sheath, the locking sleeve is locked with the sheath through threads, a first sealing ring and a second sealing ring are surrounded by the sheath, the carrying rod and the locking sleeve, the second sealing ring is positioned on a conical surface of the inner wall of the insertion end of the sheath, when the closed type ultralow temperature freezing and storing device is used, an extrusion surface in the locking sleeve extrudes the top surface of the first sealing ring to push the first sealing ring to move downwards, the first sealing ring pushes the second sealing ring to be in interference fit with the sheath and the carrying rod, and completely seals the sheath and the carrying rod by using a sealing agent. When the embryo is unfrozen, the clamping force of the first sealing ring and the second sealing ring on the carrying rod can be released by unscrewing the locking sleeve and the protective sleeve, and the carrying rod is taken out; or the loading rod is directly screwed, so that the thin-wall thawing port at the joint of the freezing hexagon and the thawing hexagon is broken, the thawing hexagon and the parts below the thawing hexagon are completely fallen off, and at the moment, the slide glass is completely exposed, and the thawed embryo can be taken out. The structure is convenient to take, does not need to use a sealant, does not pollute embryos, and is safe and reliable.

2. In the embodiment of the utility model, the electronic tag RFID is arranged in the handle part of the carrying rod, so that samples can be conveniently marked, meanwhile, the carrying rod is provided with the tag part for labeling, so that samples can be conveniently marked, and the two marking modes are more visual and accurate.

3. In the embodiment of the utility model, the loading rod is provided with the boss, so that the loading rod is prevented from rolling on the workbench, and the loading rod is protected.

Drawings

Fig. 1 is a front view of an enclosed ultra-low temperature cryogenic storage device.

Fig. 2 is a cross-sectional view of the enclosed ultra-low temperature storage apparatus taken along line D-D.

Fig. 3 is a partially enlarged view of fig. 2.

Fig. 4 is an exploded view of the enclosed ultra-low temperature cryogenic storage device.

Fig. 5 is a perspective view of the first seal ring.

Fig. 6 is a front view of the first seal ring.

Fig. 7 is a sectional view H-H of the first seal ring.

Fig. 8 is a perspective view of the second seal ring.

Fig. 9 is a cross-sectional view of the second seal ring.

Fig. 10 is a cross-sectional view of the locking sleeve.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1: as shown in fig. 1, an enclosed type ultra-low temperature freezing and storing apparatus 1 includes a sheath 2, a carrier bar 3 inserted into the sheath, and a locker 4 for locking the sheath 2 and the carrier bar 3;

2-4, the sheath 2 has an insertion end 23 provided with a first opening 21, a first cavity 22 for receiving the carrier rod 32 to be inserted therein, the first opening 21 opens into the first cavity 22, and the inner wall of the insertion end 23 is tapered;

as shown in fig. 4-10, the locking member 4 includes a locking sleeve 41, a first sealing ring 42 and a second sealing ring 43, a lower wall 411 in the locking sleeve 41 is in threaded connection with the insertion end 23 of the sheath 2, and a pressing surface 412 which is in press fit with a top surface 421 of the first sealing ring 42 is arranged in the locking sleeve 41; the first sealing ring 42 is vertically penetrated, the lower outer peripheral surface 422 of the first sealing ring 42 is a reverse conical surface, the upper inner peripheral surface 423 of the first sealing ring 42 is provided with an annular groove 424, and the lower inner peripheral surface is a first clamping surface 425 for clamping the carrier rod 3; the second seal ring 43 is an inverted frustum which is vertically penetrated, the outer peripheral surface 431 of the second seal ring 43 is an inverted conical surface, the upper inner peripheral surface 432 of the second seal ring 43 is a chamfered surface, and the lower inner peripheral surface is a second clamping surface 433 which clamps the carrier rod; the lower outer peripheral surface 422 of the first seal ring 42 is press-fitted with the chamfered surface of the upper inner peripheral surface 432 of the second seal ring 43; the carrying rod 3 is inserted into the sheath 2, the locking sleeve 41 is locked with the sheath 2 through threads, the first sealing ring 42 and the second sealing ring 43 are surrounded by the sheath 2, the carrying rod 3 and the locking sleeve 41, and the second sealing ring 43 is positioned on the conical surface of the inner wall of the insertion end 23 of the sheath 2.

As shown in fig. 4, the slide bar 3 includes a handle portion 31, a bar body 32, and a slide 33 disposed at the front end of the bar body 32 for loading embryos, and the handle portion 31 is provided at the rear end of the slide bar 3, and the handle portion 31 has a hollow square shape.

As shown in fig. 2 and 4, the carrier rod 3 further includes an electronic tag RFID34, the handle portion 31 is provided with a second opening 311 at the top end thereof, and has a second cavity 312 therein for accommodating the electronic tag RFID34, and the second opening 311 leads to the second cavity 312. The electronic label RFID34 is used for automatic identification of the sample.

As shown in fig. 2, the carrier rod 3 further includes a plug 35 for preventing the electronic tag RFID34 from falling off, and the plug 35 plugs the second opening 311 at the top end of the handle portion 31. The plug 35 can block the second opening 311 to prevent the electronic tag RFID34 from falling off, and can fix the electronic tag RFID34 in the second cavity 312 of the handle 31.

As shown in fig. 4, the carrier rod 3 further includes a boss 36 for preventing the carrier rod 3 from rolling on the worktable, the boss 36 is disposed between the rod body 32 and the handle portion 31, and the boss 36 is a rectangular parallelepiped.

As shown in fig. 4, the carrier rod 3 further includes a label portion 37 for applying a label, and the label portion 37 is disposed between the boss 36 and the handle portion 31.

As shown in fig. 4, the outer circumferential surface of the locking sleeve 41 is provided with a hexagonal cylindrical structure 413 for facilitating the screwing of the locking sleeve 41.

As shown in fig. 4, the sheath 2 further comprises a cryohexagonal head 24 for gripping the sheath 2 during use, the cryohexagonal head 24 being disposed below the threads of the insertion end 23 of the sheath 2.

As shown in fig. 4, the sheath 2 further comprises a thawing hexagonal socket 25, the thawing hexagonal socket 25 is arranged below the freezing hexagonal socket 24, and the connection part of the thawing hexagonal socket 25 and the freezing hexagonal socket 24 is a thin-wall thawing opening 26 which is convenient to break when an embryo needs to be taken out.

As shown in fig. 5, the top surface 421 of the first sealing ring 42 is a slope with a high inside and a low outside. The pressing surface 412 inside the locking sleeve 41 is pressed against the top surface of the first sealing ring 42, so that the annular groove 424 on the inner upper peripheral surface of the first sealing ring 42 is deformed, and the carrier rod 3 is clamped more firmly.

The usage of the closed type ultra-low temperature freezing storage device 1 is divided into a freezing process and a thawing process, wherein in the freezing process, the electronic tag RFID34 is firstly loaded into the second cavity 312 of the handle part 31 of the carrying rod 3, and the electronic tag RFID34 is blocked and fixed by the plug 35. Assembling the locking member 4, placing the second sealing ring 43 on the inner wall conical surface of the insertion end 23 of the sheath 2, and the inner wall conical surface is in press fit with the inverted conical surface of the outer peripheral surface 431 of the second sealing ring 43, placing the first sealing ring 42 on the second sealing ring 43, and the lower side surface 422 of the first sealing ring 42 is in press fit with the chamfered surface of the upper inner peripheral surface 432 of the second sealing ring 43, and finally connecting the inner lower wall 411 of the locking sleeve 41 with the thread of the insertion end 23 of the sheath 2, but the thread cannot be screwed at this time, so as to ensure that the pressing surface 412 in the locking sleeve 41 is separated from the top surface 421 of the first sealing ring 42. The method comprises the steps of loading samples such as embryos or gametes needing to be refrigerated on a slide glass 33 of a carrying rod 3, inserting the carrying rod 3 into the assembled locking part 4 and the assembled sheath 2 to complete the assembly of the closed type ultralow-temperature freezing storage device 1, placing the closed type ultralow-temperature freezing storage device 1 in liquid nitrogen, placing a jig 1 (not shown in the figure) in the liquid nitrogen, matching a freezing hexagonal socket 24 of the sheath 2 with the jig 1 to fix the closed type ultralow-temperature freezing storage device 1, and ensuring that the liquid level of the liquid nitrogen is lower than a first opening 21 of an insertion end 23 of the sheath 2 to prevent the liquid nitrogen from entering the sheath 2 to pollute the samples. Since the fixture 1 is matched with the freezing hexagon 24 on the sheath 2, so as to clamp the whole sheath 2, when the hexagonal column structure 413 on the locking sleeve 41 is rotated, the extrusion surface 412 in the locking sleeve 41 extrudes the top surface 421 of the first sealing ring 42, so that the annular groove 424 on the inner circumferential surface of the first sealing ring 42 is deformed, the first clamping surface 425 on the lower inner circumferential surface of the first sealing ring 42 is forced to clamp the carrying rod 3, meanwhile, the lower outer circumferential surface 422 of the first sealing ring 42 presses the upper inner circumferential surface 432 of the second sealing ring 43, so that the second clamping surface 433 on the lower inner circumferential surface of the second sealing ring 43 is forced to clamp the carrying rod 3, and the carrying rod 3, the sheath 2 and the locking member 4 are in interference fit, so that the sealing process is completed and the whole fixture is placed in a liquid nitrogen tank. Because the first sealing ring 42 and the second sealing ring 43 are made of special metal, the deformation of the first sealing ring and the second sealing ring is still elastic deformation under the condition of temperature difference, and the sealing effect of the device during working is ensured.

In the thawing process, the closed type ultra-low temperature freezing storage device 1 is taken out of liquid nitrogen, the thawing hexagonal socket 25 is placed on the jig 2 (not shown in the figure), the sheath 2 and the closed type ultra-low temperature freezing storage device 1 are fixed, at the moment, the handle part 31 is held by hand, the carrying rod 3 is slightly rotated, the thin-wall thawing opening 26 between the freezing hexagonal socket 24 and the thawing socket 25 on the sheath 2 is broken, at the moment, the liquid level of the liquid nitrogen is lower than the thin-wall thawing opening 26, the liquid nitrogen cannot flow into the sheath 2 to pollute a sample, the thawing hexagonal socket 25 and the parts below on the sheath 2 are all fallen off, the carrier 3 is taken out, and the sample thawing operation is carried out.

While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the utility model.

Claims

1. A closed ultra-low temperature freezing and storing device is characterized in that the freezing and storing device comprises a sheath, a carrying rod inserted into the sheath, and a locking piece used for locking the sheath and the carrying rod;

2. A closed ultra-low temperature frozen storage device as claimed in claim 1, wherein said carrier bar comprises a handle portion, a bar body and a slide for loading embryos provided at a front end of the bar body, and a handle portion is provided at a rear end of the carrier bar.

3. A closed ultra-low temperature cryogenic storage device as claimed in claim 2 further comprising an electronic tag RFID, said handle portion having a second opening at a top end thereof and a second cavity therein for receiving the electronic tag RFID, the second opening leading to the second cavity.

4. A closed ultra-low temperature freezing storage device as claimed in claim 3, further comprising a stopper for preventing the electronic tag RFID from falling, the stopper blocking the second opening at the top end of the handle portion.

5. A closed ultra-low temperature storage apparatus as claimed in claim 2, further comprising a boss for preventing the loading bar from rolling on the table, the boss being disposed between the lever body and the handle portion.

6. A closed ultra-low temperature freezing storage device as claimed in claim 5, further comprising a label portion for labeling, the label portion being disposed between the boss and the handle portion.

7. A closed ultra-low temperature freezing storage device as claimed in claim 1, wherein the locking sleeve is provided at its outer peripheral surface with a hexagonal cylinder structure for facilitating screwing of the locking sleeve.

8. A closed, ultra-low temperature cryogenic storage device as claimed in claim 1, wherein the sheath further comprises cryohexagons for gripping the sheath during use, the cryohexagons being disposed below the threads of the insertion end of the sheath.

9. A closed ultra-low temperature frozen storage device as claimed in claim 8, wherein the sheath further comprises a thawing hexagonal socket, the thawing hexagonal socket is disposed below the freezing hexagonal socket, and the connection between the thawing hexagonal socket and the freezing hexagonal socket is a thin wall thawing opening for facilitating the disconnection of embryos when the embryos are to be removed.

10. A closed ultra-low temperature cryogenic storage device as claimed in claim 1, wherein the top surface of the first sealing ring is a slope with a high inside and a low outside.