CN216688122U

CN216688122U - Strain preservation tube

Info

Publication number: CN216688122U
Application number: CN202123433517.4U
Authority: CN
Inventors: 陈佳; 穆燕魁
Original assignee: Hebei Gaoye Technology Co ltd
Current assignee: Hebei Gaoye Technology Co ltd
Priority date: 2021-12-30
Filing date: 2021-12-30
Publication date: 2022-06-07
Anticipated expiration: 2031-12-30

Abstract

The utility model discloses a strain preservation tube which comprises a tube body and a tube cover matched with the tube body for use, wherein an inoculating ring extending into the tube body is fixed in the tube cover, a flow guide groove is formed in the inner wall of the tube body, the depth of the flow guide groove is gradually deepened towards the opening of the tube body along the bottom of the tube body, a frozen stock solution is added into the tube body, and ceramic beads used for packaging strains are soaked in the frozen stock solution. The utility model can discharge bacterial suspension in the tube body as clean as possible.

Description

Strain preservation tube

Technical Field

The utility model relates to the field of strain preservation, in particular to a strain preservation tube.

Background

The strain preservation tube can preserve general bacteria, harsh bacteria, mould, saccharomycetes and the like, the low-temperature cryopreservation of the porcelain beads is also a common preservation mode, when the strain preservation tube is used, an oily white board pen is generally used for marking the name of the strain on each strain preservation tube, a spiral bottle cover of the strain preservation tube is opened under the aseptic condition, a pure culture of a fresh strain (18-24 hours) is picked by an inoculating ring and is put into a cryopreservation solution, then the bottle cover is screwed down, the strain is emulsified by reversing the strain back and forth to prepare a bacterial suspension with the turbidity of about 3-4 McLeeb, the bacterial suspension can be adsorbed on the inner surface and the outer surface of the small porcelain beads, then an aseptic pipettor is used for sucking the bacterial suspension in the strain preservation tube as much as possible, the bottle cover is screwed down, the detailed condition of the inoculated strain is recorded, and the strain preservation tube after inoculation is put in a refrigerator at the temperature of-20 ℃ or-80 ℃.

However, when the bacterial suspension is sucked out, the bacterial suspension often remains in the tube body because the ceramic beads directly contact with the bottom of the tube body, and the bacterial suspension cannot be completely absorbed, so that the ceramic beads are frozen together in the freezing process, and the taking is difficult.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the utility model provides a strain preservation tube which can discharge bacterial suspension in the tube body as clean as possible.

In order to achieve the purpose, the technical scheme adopted by the utility model is as follows:

the utility model provides a pipe is preserved to bacterial, including the body and with the tube cap that the body cooperation was used, the tube cap internal fixation to the transfering loop that extends in the body seted up the guiding gutter on the inner wall of body, the degree of depth of guiding gutter is followed the bottom of body to the opening part of body deepens gradually, the body has added frozen stock solution, it has the porcelain pearl that is used for encapsulating the bacterial to soak in the frozen stock solution.

Optionally, the diameter of the ceramic beads is larger than the width of the flow guide groove.

Optionally, a round hole for clamping is formed in the middle of the ceramic bead, and a plurality of small holes for adsorbing bacteria are formed in the surface of the ceramic bead.

Optionally, the outer wall of the pipe body is pasted with a label and marked with scale marks.

Optionally, the pipe cover is externally provided with anti-slip lines.

Optionally, the bottom of body is the tip conical shape downwards, the bottom of body still is equipped with the conduit saddle, the outside of conduit saddle is equipped with a plurality of strengthening ribs.

Optionally, the inner surface of the pipe cover is provided with an external thread, and the open end of the pipe body is provided with an internal thread matched with the external thread.

Compared with the prior art, the utility model has the technical progress that:

the utility model is provided with a pipe body and a pipe cover, wherein the inner wall of the pipe body is provided with a flow guide groove, ceramic beads are arranged in the pipe body, bacterial suspension can firstly flow into the flow guide groove, after most of the bacterial suspension is sucked out, the pipe body is inclined or laid flat, because the depth of the flow guide groove is gradually deepened towards the opening of the pipe body along the bottom of the pipe body, and a little of the residual bacterial suspension can flow to the opening of the pipe body along the flow guide groove, so that the bacterial suspension in the pipe body is sucked as clean as possible. Meanwhile, a taking ring extending inwards the tube body is fixed in the tube cover, when the preserved strains are taken out and inoculated, the taking ring can be directly used for placing the porcelain beads into the taking ring and then taken out, and an inoculating ring (an inoculating needle) or tweezers are not needed.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model.

In the drawings:

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a cross-sectional view of the tube of the present invention.

In the figure:

the method comprises the following steps of 1-pipe body, 2-pipe cover, 3-taking ring, 4-flow guide groove, 5-ceramic bead, 6-anti-skid grain, 7-pipe support and 8-reinforcing rib.

Detailed Description

The following several specific embodiments may be combined with each other, and details of the same or similar concepts or processes may not be repeated in some embodiments. Embodiments of the present invention will be described below with reference to the accompanying drawings.

The utility model discloses a strain preservation tube by combining a figure 1 and a figure 2, which comprises a tube body 1 and a tube cover 2 matched with the tube body 1, wherein a taking ring 3 extending into the tube body 1 is fixed in the tube cover 2, when preserved strains are taken out and inoculated, porcelain beads 5 can be directly placed into the taking ring 3 by using the taking ring 3 and then taken out, an inoculating ring (inoculating needle) or tweezers are not needed, the inoculating ring (inoculating needle) or the tweezers are not needed to be sterilized, the time and the labor are saved, and the possibility of strain pollution is greatly reduced. A diversion trench 4 is arranged on the inner wall of a tube body 1, the depth of the diversion trench 4 is gradually deepened towards the opening of the tube body 1 along the bottom of the tube body 1, a frozen stock solution is added into the tube body 1, porcelain beads 5 for encapsulating strains are soaked in the frozen stock solution, a pure culture of fresh strains (18-24 hours) is picked by an inoculating loop and put into the frozen stock solution, then screwing the tube cover 2, reversing to emulsify the bacteria to prepare bacterial suspension with turbidity of about 3-4 McLeod, absorbing the bacterial suspension on the inner and outer surfaces of the porcelain beads 5, sucking out the bacterial suspension in the strain preservation tube by using a sterile pipettor as much as possible, after most of the bacterial suspension is sucked out, the pipe body 1 is inclined or leveled, because the depth of the flow guide groove 4 is gradually deepened towards the opening of the pipe body 1 along the bottom of the pipe body 1, a little of the residual bacterial suspension can flow to the opening of the pipe body 1 along the flow guide groove 4, and therefore the bacterial suspension in the pipe body 1 is sucked up as completely as possible.

Furthermore, the diameter of the porcelain beads 5 is larger than the width of the diversion trench 4, so that the porcelain beads 5 are prevented from directly contacting with the bottom of the tube body 1, and the bacterial suspension can flow into the diversion trench 4 more quickly.

Furthermore, a round hole for clamping is formed in the middle of the ceramic bead 5, a plurality of small holes for adsorbing bacteria are formed in the surface of the ceramic bead, and the round hole in the middle of the ceramic bead 5 can be used for clamping a inoculating loop (inoculating needle) or tweezers when the taking ring 3 is not used. Meanwhile, the small holes formed in the surface of the ceramic beads 5 can adsorb more bacteria, and the storage rate of the strains is increased.

Furthermore, the outer wall of the tube body 1 is pasted with a label and marked with scale marks, so that the marking and the metering of the strain storage tube are facilitated.

Furthermore, the pipe cover 2 is externally provided with anti-skid grains 6, so that an operator can conveniently open the pipe cover.

Further, the bottom of body 1 is tip conical downwards, and the bottom of body 1 still is equipped with conduit saddle 7, and the outside of conduit saddle 7 is equipped with a plurality of strengthening ribs 8, makes things convenient for handheld strengthening rib 8 of operating personnel, has avoided the temperature of hand influence bacterial in the bacterial storage tube temperature.

Furthermore, the inner surface of the pipe cover 2 is provided with an external thread, and the open end of the pipe body 1 is provided with an internal thread matched with the external thread.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A strain storage tube, comprising: body (1) and with tube cap (2) that body (1) cooperation was used, tube cap (2) internal fixation to ring (3) of taking that extends in body (1) seted up guiding gutter (4) on the inner wall of body (1), the degree of depth of guiding gutter (4) is followed the bottom of body (1) to the opening part of body (1) deepens gradually, body (1) has added frozen stock liquid, it has porcelain pearl (5) that are used for the encapsulation bacterial to soak in the frozen stock liquid.

2. A seed holding tube as defined in claim 1, wherein: the diameter of the porcelain beads (5) is larger than the width of the diversion trench (4).

3. A seed holding tube as defined in claim 2, wherein: a round hole used for clamping is formed in the middle of the ceramic bead (5), and a plurality of small holes used for adsorbing bacteria are formed in the surface of the ceramic bead (5).

4. A seed holding tube as defined in claim 1, wherein: the outer wall of the pipe body (1) is pasted with a label and marked with scale marks.

5. A seed holding tube as defined in claim 1, wherein: and anti-skid grains (6) are arranged outside the pipe cover (2).

6. A seed holding tube as defined in claim 1, wherein: the bottom of body (1) is the tip conical shape down, the bottom of body (1) still is equipped with conduit saddle (7), the outside of conduit saddle (7) is equipped with a plurality of strengthening ribs (8).

7. A seed holding tube as defined in claim 1, wherein: the inner surface of the pipe cover (2) is provided with an external thread, and the open end of the pipe body (1) is provided with an internal thread matched with the external thread.