CN216687966U

CN216687966U - Protein crystallization device with high air permeability and capability of improving protein crystallization rate

Info

Publication number: CN216687966U
Application number: CN202122508819.7U
Authority: CN
Inventors: 阳丽艳; 杨登峰; 潘丽霞; 李红亮
Original assignee: Guangxi Baierfa Bioengineering Co ltd
Current assignee: Guangxi Baierfa Bioengineering Co ltd
Priority date: 2021-10-19
Filing date: 2021-10-19
Publication date: 2022-06-07
Anticipated expiration: 2031-10-19

Abstract

The utility model discloses a protein crystallization device with high air permeability and capable of improving the protein crystallization rate, which comprises a shell and a handle, wherein a supporting seat is arranged at the bottom of the shell, and a box door is arranged on the front side of the shell through an opening and closing leaf; a ventilation shell penetrates through one side of the shell, a ventilation fan is arranged on the inner side of the ventilation shell, and a filter layer is arranged on the inner side of the ventilation shell; the front of chamber door is installed the peeping window, the front of chamber door is installed the handle. The air exchange shell is provided with the air exchange fan and the filter layer, after the device is electrified, the air exchange fan drives the fan blades arranged in the air exchange fan to rotate through an electromagnetic effect, so that suction force is generated on air in an external environment, the air is discharged to the inside of the filter layer, the filter layer is formed by overlapping multiple layers of filter paper, when the air enters the air exchange fan under the pressure action, the filter layer filters dust and bacteria in the air, and the influence of mixed bacteria on the protein crystallization efficiency is prevented while the air enters the shell.

Description

Protein crystallization device with high air permeability and capability of improving protein crystallization rate

Technical Field

The utility model relates to the technical field of protein crystallization, in particular to a protein crystallization device which has high air permeability and can improve the protein crystallization rate.

Background

With the continuous development of modern society and the continuous improvement of economic level, research on scientific research in modern society is increasing, and in the existing scientific technology, bioscience is also developing gradually as a technology for researching life-bearing substances such as life, cells, DNA, protein and the like, while in the technology of bioshells, protein is used as the embodiment of life activities, the structure of the protein determines the function of the protein, and in the process of the existing protein, the protein is required to be crystallized, so that the protein can be analyzed by rays, and on the basis of the crystallization, a protein crystallization device is required to crystallize a saturated protein solution.

The prior art protein crystallization device has the defects that:

1. the utility model discloses a gas-permeable crystallization device and a method for protein crystallization under the device, and the utility model provides the gas-permeable crystallization device and the method for protein crystallization under the device, wherein a crystallization plate and a transparent sealing part are made of gas-permeable and transparent materials, and only a liquid storage tank for placing a protein crystallization solution is arranged in the crystallization plate to form a plurality of mutually independent crystallization sealing spaces. The utility model obviously improves the success rate of screening protein crystallization conditions, reduces the cost of protein crystallization, has simple structure, lower manufacturing cost, simple method operation and better appearance of the grown protein crystal, and can not ventilate the saturated protein solution in the device in the using process of the device due to the lack of an auxiliary ventilation part, thereby influencing the crystallization efficiency of the device.

2. The existing protein crystallization device is lack of a device humidity control component, so that the humidity inside the device cannot be controlled in the use process of the device, and the crystallization efficiency of the device is influenced.

SUMMERY OF THE UTILITY MODEL

The present invention has been made to solve the above-mentioned problems occurring in the prior art, and an object of the present invention is to provide a protein crystallization apparatus having high gas permeability and capable of increasing the protein crystallization rate.

In order to achieve the purpose, the utility model provides the following technical scheme: a protein crystallization device with high air permeability and capable of improving the protein crystallization rate comprises a shell and a handle, wherein a supporting seat is arranged at the bottom of the shell, and a box door is arranged on the front side of the shell through an opening and closing sheet;

a ventilation shell is installed on one side of the shell in a penetrating mode, a plurality of ventilation holes are formed in the outer surface of the ventilation shell, a ventilation fan is installed on the inner side of the ventilation shell, a filter layer is installed on the inner side of the ventilation shell, and the filter layer is located on the other side of the ventilation fan;

the front of the box door is provided with a peeping window, the front of the box door is provided with a handle, and the handle is positioned at the other side of the peeping window.

Preferably, two sets of slide rail bars are installed inside the housing, and the inner sides of the slide rail bars are slidably connected with a frame.

Preferably, the inner side of the frame is connected with a screen, and the top of the screen is provided with a crystallization box.

Preferably, a humidity controller is mounted on the outer surface of the other side of the housing.

Preferably, a water tank is installed on the back of the shell, and a delivery pump is installed at the top of the water tank; and the delivery pump is electrically connected with the humidity controller.

Preferably, the output end of the delivery pump is provided with an output pipe, and the tail end of the output pipe extends to the inside of the shell.

Preferably, the tail end of the output pipe is provided with an atomizing nozzle.

Compared with the prior art, the utility model has the following beneficial effects:

1. the air exchange fan and the filter layer are arranged, after the device is electrified, the air exchange fan converts electric energy into kinetic energy through an electromagnetic effect, the fan blades arranged in the air exchange fan are driven to rotate, suction force is generated on air in an external environment, the air is discharged to the inside of the filter layer, the filter layer is formed by overlapping multiple layers of filter paper, the filter layer has good filtering performance, when the air enters the air exchange fan under the pressure effect, the filter layer filters dust and bacteria in the air, and the influence of the bacteria on protein crystallization efficiency is prevented while the air enters the shell.

2. The utility model sets the humidity sensing upper limit of the humidity controller according to the protein crystallization requirement after the device is electrified, when the humidity in the shell is lower than the sensing upper limit, the humidity controller conducts current to the interior of the conveying pump, the conveying pump generates suction force to cold water in the water tank and discharges the cold water to the interior of the output pipe, the output pipe conveys the cold water to the interior of the atomizing nozzle under the pressure of the output end of the conveying pump, when the cold water enters the interior of the atomizing nozzle under the pressure of the conveying pump, the atomizing nozzle atomizes and sprays the cold water under the pressure of the output end of the conveying pump to increase the humidity in the shell, when the humidity in the shell reaches the sensing upper limit, the humidity controller cuts off the power of the conveying pump, thereby controlling the humidity of the protein to be crystallized, the crystallization of the protein is accelerated.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the overall structure of the back side of the present invention;

FIG. 3 is a schematic view of the structure of the other side outer surface part of the shell of the present invention;

FIG. 4 is a cross-sectional partial structural schematic view of the housing of the present invention;

FIG. 5 is a schematic illustration of a portion of a screen according to the present invention;

FIG. 6 is a schematic view of the top interior wall portion of the housing of the present invention;

fig. 7 is a cross-sectional partial structural view of the ventilation housing of the present invention.

In the figure: 1. a housing; 2. a supporting seat; 3. a box door; 4. ventilating the shell; 5. a ventilator; 6. a filter layer; 7. snooping the window; 8. a grip; 9. a slide rail bar; 10. a frame; 11. screening a screen; 12. a crystallization box; 13. A humidity controller; 14. a water tank; 15. a delivery pump; 16. an output pipe; 17. an atomizing spray head.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, fig. 2, fig. 3 and fig. 4, an embodiment of the present invention: a protein crystallization device with high air permeability and capable of improving the protein crystallization rate.

In the first embodiment, the device comprises a shell 1 and a handle 8, wherein a supporting seat 2 is installed at the bottom of the shell 1, the shell 1 provides fixing points for the supporting seat 2, a box door 3, a ventilation shell 4, a sliding rail strip 9, a humidity controller 13 and a water tank 14 which are installed on the outer surface and inside of the shell 1, the supporting seat 2 is fixed at the bottom of the shell 1 to provide support for the whole device, and the box door 3 is installed on the front surface of the shell 1 through an opening and closing sheet; the front surface of the box door 3 is provided with a peeping window 7, the peeping window 7 is made of glass material and has good light transmittance, a user can conveniently check the condition inside the shell 1 in real time, the front surface of the box door 3 is provided with a handle 8, the handle 8 is positioned at the other side of the peeping window 7, when the device is needed, the handle 8 is pulled by external force, the handle 8 drives the opening hinge to rotate to open the box door 3, two groups of slide rail bars 9 are arranged inside the shell 1, the slide rail bars 9 provide connection points for a frame 10 connected with the inner side of the slide rail bars 9, meanwhile, the packaging frame 10 has mobility when being pulled by external force, the inner side of the slide rail bars 9 is connected with a frame 10 in a sliding way, when the box door 3 is opened, the frame 10 is pulled by external force, the frame 10 moves outwards along the slide rail bars 9 at the moment, the crystallization box 12 is driven to move outwards, and the inner side of the frame 10 is connected with a screen 11, a crystallization cassette 12 is installed on the top of the screen 11, and a saturated protein solution is dropped into the crystallization cassette 12 by an external force after the crystallization cassette 12 is moved to a proper position.

As shown in FIGS. 1 and 4, a protein crystallization apparatus having high gas permeability and capable of increasing the protein crystallization rate,

in the second embodiment, an air exchange shell 4 is installed on one side of the casing 1 in a penetrating manner, a plurality of air exchange holes are formed in the outer surface of the air exchange shell 4, the inside of the casing 1 is communicated with the external environment through the air exchange holes formed in the outer surface of the air exchange shell 4, the inside of the casing 1 can exchange gas with the external environment, an air exchange fan 5 is installed on the inner side of the air exchange shell 4, after the device is powered on, the air exchange fan 5 converts electric energy into kinetic energy through an electromagnetic effect, blades installed in the air exchange fan are driven to rotate, air in the external environment is sucked, and the air is discharged into the inside of a filter layer 6, the filter layer 6 is installed on the inner side of the air exchange shell 4, and the filter layer 6 is located on the other side of the air exchange fan 5, and is manufactured by overlapping multiple layers of filter paper, so that the air exchange fan has good filtering performance, when the air enters the inside of the air exchange fan 5 under the pressure, the filter layer 6 filters dust and bacteria in the air, the air is ensured to enter the shell 1, and meanwhile, the influence of bacteria on the protein crystallization efficiency is prevented.

As shown in FIGS. 1 and 3, a protein crystallization apparatus having high gas permeability and capable of increasing the protein crystallization rate,

in the third embodiment, a humidity controller 13 is installed on the outer surface of the other side of the housing 1, after the protein saturated solution is injected, the upper humidity sensing limit of the humidity controller 13 is set according to the protein crystallization requirement, when the humidity inside the housing 1 is lower than the upper humidity sensing limit, the humidity controller 13 conducts current to the inside of the delivery pump 15, when the humidity inside the housing 1 reaches the upper humidity sensing limit, the delivery pump 15 is powered off by the humidity controller 13, a water tank 14 is installed on the back of the housing 1, cold water is stored inside the water tank 14, the delivery pump 15 is installed on the top of the water tank 14, the delivery pump 15 is electrically connected with the humidity controller 13, when the electric energy is conducted to the inside of the delivery pump 15 through the humidity controller 13, the delivery pump 15 generates suction to the cold water inside the water tank 14 and discharges the cold water to the inside of the output pipe 16, the output end of the delivery pump 15 is installed with the output pipe 16, and the tail end of the output pipe 16 extends to the inside of the shell 1, the output pipe 16 is connected with the atomizing nozzle 17 and the delivery pump 15, cold water is delivered to the inside of the atomizing nozzle 17 under the pressure of the output end of the delivery pump 15, the atomizing nozzle 17 is installed at the tail end of the output pipe 16, and when the cold water enters the inside of the atomizing nozzle 17 under the pressure action of the delivery pump 15, the atomizing nozzle 17 atomizes and sprays the cold water under the pressure action of the output end of the delivery pump 15, so that the humidity inside the shell 1 is increased.

The working principle is as follows: firstly, pulling a handle 8 by external force, at the moment, the handle 8 drives an opening hinge to rotate so as to open a box door 3, then pulling a frame 10 by the external force, at the moment, the frame 10 moves outwards along a slide rail strip 9 to drive a crystallization box 12 to move outwards, after the crystallization box 12 moves to a proper position, dropwise adding a saturated protein solution into the crystallization box 12 by the external force, then closing the box door 3, connecting a power supply, setting a humidity sensing upper limit of a humidity controller 13 according to the crystallization requirement of protein, when the humidity in a shell 1 is lower than the sensing upper limit, the humidity controller 13 transmits current to the inside of a delivery pump 15, at the moment, the delivery pump 15 generates suction on cold water in a water tank 14 and discharges the cold water to the inside of an output pipe 16, the output pipe 16 delivers the cold water to the inside of an atomizing nozzle 17 under the pressure at the output end of the delivery pump 15, and when the cold water enters the inside of the atomizing nozzle 17 under the pressure of the delivery pump 15, the cold water is atomized and sprayed out by the atomizing nozzle 17 under the pressure action of the output end of the delivery pump 15, the humidity inside the shell 1 is increased, and when the humidity inside the shell 1 reaches the sensing upper limit, the humidity controller 13 enables the delivery pump 15 to be powered off, so that the humidity of the protein to be crystallized is controlled, and the crystallization of the protein is accelerated.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. A protein crystallization device with high air permeability and capable of improving protein crystallization rate, which comprises a shell (1) and a handle (8), and is characterized in that: the bottom of the shell (1) is provided with a supporting seat (2), and the front of the shell (1) is provided with a box door (3) through an opening and closing leaf;

a ventilation shell (4) penetrates through one side of the shell (1), a plurality of ventilation holes are formed in the outer surface of the ventilation shell (4), a ventilation fan (5) is installed on the inner side of the ventilation shell (4), a filter layer (6) is installed on the inner side of the ventilation shell (4), and the filter layer (6) is located on the other side of the ventilation fan (5); the front of chamber door (3) is installed peeping window (7), handle (8) are installed in the front of chamber door (3), and handle (8) are located the opposite side of peeping window (7).

2. The protein crystallization apparatus having high gas permeability and capable of increasing the crystallization rate of protein according to claim 1, wherein: the inside of casing (1) is installed two sets of skid rails (9), the inboard sliding connection of skid rails (9) has frame (10).

3. The protein crystallization apparatus having high gas permeability and capable of increasing the crystallization rate of protein according to claim 2, wherein: the inner side of the frame (10) is connected with a screen (11), and a crystallization box (12) is installed at the top of the screen (11).

4. The protein crystallization apparatus according to claim 1, wherein the apparatus has a high gas permeability and is capable of increasing the crystallization rate of the protein, and further comprises: and a humidity controller (13) is arranged on the outer surface of the other side of the shell (1).

5. The protein crystallization apparatus according to claim 1, wherein the apparatus has a high gas permeability and is capable of increasing the crystallization rate of the protein, and further comprises: a water tank (14) is mounted on the back of the shell (1), and a delivery pump (15) is mounted at the top of the water tank (14); and the delivery pump (15) is electrically connected with the humidity controller (13).

6. The protein crystallization apparatus according to claim 5, wherein the gas permeability is high and the protein crystallization rate is increased, and the apparatus further comprises: an output pipe (16) is installed at the output end of the delivery pump (15), and the tail end of the output pipe (16) extends to the inside of the shell (1).

7. The protein crystallization apparatus according to claim 6, wherein the gas permeability is high and the protein crystallization rate is increased, and the apparatus further comprises: and the tail end of the output pipe (16) is provided with an atomizing nozzle (17).