CN216669464U

CN216669464U - Microscopical system appearance device

Info

Publication number: CN216669464U
Application number: CN202123076142.0U
Authority: CN
Inventors: 王雷; 倪磊; 郦野; 金帆
Original assignee: Shenzhen Institute of Advanced Technology of CAS; Shenzhen Technology University
Current assignee: Shenzhen Institute of Advanced Technology of CAS; Shenzhen Technology University
Priority date: 2021-12-07
Filing date: 2021-12-07
Publication date: 2022-06-03
Anticipated expiration: 2031-12-07

Abstract

The utility model discloses a microscopic sample preparation device which comprises eight parts, namely a sample pool plate assembly, an agar pouring plate assembly, a light homogenizing assembly, a light filtering assembly, an LED lamp plate fixing frame, ultrathin glass and a glass pressing ring. The sample cell plate is used for fixing ultrathin glass and is used as a glass slide in tabletting, and the agar pouring plate is used for manufacturing an agar block for tabletting. The LED lamp plate provides the light source, and even light subassembly comprises plano-convex lens for light is more even. The filtering component is composed of a filter and can select light with required wavelength. The components are stacked and assembled together in a magnetic attraction mode, and the assembly is convenient. The sample preparation process is simple and quick, is easy to master by beginners, and can ensure the tabletting effect. The whole device can provide a medium-flux sample experiment and can meet the experimental requirements of optogenetics.

Description

Microscopical system appearance device

Technical Field

The utility model belongs to the technical field of experimental instruments, and relates to a biological or medical sample preparation device, in particular to a microscopic sample preparation device.

Background

During biological or medical experiments, individual bacteria or cells are observed on a microscope for better visualization. The following sampling procedure is often employed: the bacteria/cell suspension was dropped onto the cut agar medium block. Waiting for the agar surface to air-dry slightly, pressing the side of the agar block dropped with the cell suspension on a glass cover glass. The cells or bacteria can then be observed on an inverted microscope and the process described above is referred to as a pelleting process. Wherein the agar block is cut in the operation and the bacteria/cell suspension is dropped on it, and finally the agar block with the suspension is pressed on the cover glass. The experimental personnel needs to carefully operate the test specimen to obtain a specimen with good effect. The entire experimental procedure would be extremely cumbersome and inefficient in the testing process in the face of multiple experimental samples. It takes a lot of time and effort for laboratory staff, and misoperation is easy to occur, which seriously affects the progress of scientific research or medical diagnosis.

In the current biological cell experiment process, the preparation process of a microscopic sample is generally manually operated, the prepared solid agar is cut into small blocks by a knife, then the bacterial/cell suspension is dripped on the agar blocks, and the agar blocks are pressed on a glass cover glass and placed on a microscope to observe cells. For experimenters, the operation of the whole process needs great care to ensure good tabletting effect. Only one sample can be prepared at a time, and a lot of time and energy are needed in the process of multi-sample experiment. In addition, the operation needs a certain skill, and the tabletting sample which is easy to be made by a novice can not meet the requirement and needs to be made again, thus seriously influencing the experiment progress. In addition, the glass cover glass is only less than 0.2 mm thick and is very easy to crack in the operation process, so that the experiment fails and even the hands of experimenters are cut, and the danger is caused.

In conclusion, the prior art of the existing sheet making process is that a single sample is made manually, and the problems of complex operation process and high labor and time cost exist.

Disclosure of Invention

The utility model provides a microscopic sample preparation device, which solves the problems of inconvenient operation in the tabletting process of a biological cell experiment, long preparation time of an experimental sample and low efficiency. Meanwhile, an optical control device is added, and a solution is provided for researchers in optogenetics (optogenetics) to test different light control intensities and light control curves for photosensitive strains.

In order to achieve the purpose, the utility model adopts the following technical scheme to realize the purpose:

a microsampling device, comprising:

the LED lamp panel fixing frame comprises an annular frame, the inner ring is provided with a plurality of bulges facing the circle center of the annular frame, the round LED lamp panel is arranged on the inner bottom surface of the LED lamp panel fixing frame, and the top surface of the LED lamp panel is abutted against the bulges of the inner ring of the LED lamp panel fixing frame;

the light homogenizing assembly is arranged below the LED lamp panel and buckled with the LED lamp panel fixing frame to clamp the LED lamp panel; the light homogenizing assembly is provided with a plano-convex lens mounting hole corresponding to the position of an LED lamp bead on the LED lamp panel, and a plano-convex lens is arranged in the plano-convex lens mounting hole;

the light filtering component is arranged below the light uniformizing component and is buckled with the light uniformizing component; the optical filter assembly is provided with an optical filter mounting hole corresponding to the position of the plano-convex mirror mounting hole, and an optical filter is arranged in the optical filter mounting hole;

the agar pouring plate is arranged below the light filtering component and buckled with the light filtering component; a first agar pouring hole corresponding to the position of the optical filter mounting hole is formed in the agar pouring plate;

the sample pool plate is arranged below the agar pouring plate and is buckled with the agar pouring plate; a second agar pouring hole corresponding to the first agar pouring hole is formed in the sample cell plate; the bottom surface of the sample cell plate is provided with a glass sheet;

and the glass pressing ring is arranged below the sample cell plate and is buckled with the sample cell plate, and the glass sheet is tightly pressed and attached to the sample cell plate.

Compared with the prior art, the utility model has the following beneficial effects:

the present invention addresses these shortcomings by providing a slide making apparatus suitable for use with a microscope stage that is sized to fit within a particular microscope fixture. Thus, from the conventional apparatus in which only one test sample can be observed at a time, it is now possible to perform the observation of 7 test samples at a time. Can complete rapid sample preparation and observation of a small number of samples. The utility model can simplify the sample preparation process, so that a beginner can complete the sample preparation process very quickly, and the sample preparation effect is good. The porous design meets the experiment requirement of the medium flux sample in the actual experiment. The plano-convex lens enables the LED light source to be more uniform, and the optical filter is used for selecting light rays with required wave bands. Utilize software can control the illumination colour and the intensity of LED lamp plate lamp pearl, mutual independence between the porous satisfies the optogenetics experiment of different demands.

Drawings

In order to more clearly explain the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

Fig. 1 is a schematic view of the assembly relationship of the overall structure of the present invention.

Fig. 2 is an isometric view of the overall structure of the present invention.

Fig. 3 is a front view of the overall structure of the present invention.

Fig. 4 is a schematic structural view of the LED lamp panel fixing frame of the present invention.

Fig. 5 is a schematic structural diagram of the LED lamp panel according to the present invention.

Fig. 6 is a schematic view of an assembly structure of the LED lamp panel fixing frame, the LED lamp panel and the light uniformizing assembly according to the present invention.

FIG. 7 is a schematic view of an assembly relationship of the LED lamp panel fixing frame, the LED lamp panel and the light homogenizing assembly.

FIG. 8 is a schematic view of an assembly structure of the dodging assembly of the present invention.

FIG. 9 is a schematic view of a reverse structure of the first light uniformizing plate according to the present invention.

FIG. 10 is a schematic front view of a first uniform light plate according to the present invention.

FIG. 11 is a schematic view of a reverse structure of a second light distribution plate according to the present invention.

FIG. 12 is a schematic front view of a second light distribution plate according to the present invention.

FIG. 13 is a cross-sectional view of the light unifying assembly of the present invention.

Fig. 14 is a schematic view of an assembly structure of the filter assembly of the present invention.

FIG. 15 is a schematic diagram of a reverse structure of the first filter plate according to the present invention.

FIG. 16 is a schematic front view of a first filter plate according to the present invention.

FIG. 17 is a schematic diagram of a second filter plate according to the present invention.

FIG. 18 is a schematic front view of a second filter plate according to the present invention.

Fig. 19 is a cross-sectional view of a filter assembly of the present invention.

FIG. 20 is an isometric view of an agar casting plate of the utility model.

FIG. 21 is a schematic front view of a sample cell plate according to the present invention.

FIG. 22 is a schematic view of the reverse structure of the sample cell plate according to the present invention.

FIG. 23 is a schematic view showing the structure of the agar casting plate and the sample well plate according to the present invention.

FIG. 24 is a schematic view of the structure of the glass sheet and glass press ring of the present invention.

FIG. 25 is a cross-sectional view of a sample cell plate mounting glass plate and glass press ring of the present invention.

FIG. 26 is a schematic view showing the assembly of the agar pressing plate of the present invention.

FIG. 27 is a schematic view showing the structure of the agar pressing plate of the present invention for pressing agar.

FIG. 28 is a schematic view showing the structure of an agar pressing plate according to the present invention.

FIG. 29 is a graph showing the relationship between the size of the pressing projection and the aperture of the agar casting plate and the well plate according to the present invention.

FIG. 30 is a graph showing the relationship between the pressing protrusions and the depth of the holes in the agar casting plate and the well plate according to the present invention.

FIG. 31 is a schematic view showing the assembly of the present invention with a sample holding disk.

FIG. 32 is a schematic view showing the structure of a sample-fixing disk according to the present invention.

Wherein:

1 is an LED lamp panel fixing frame, and 1-1 is an arc-shaped buckle;

2, an LED lamp panel;

3, a light homogenizing component, 3-1, a first light homogenizing plate, 3-2, a second light homogenizing plate, 3-3, a first magnet mounting hole, 3-4, a first plano-convex lens mounting hole, 3-5, an LED lamp panel positioning column, 3-6, a first buckle, 3-7, a second magnet mounting hole, 3-8, a second plano-convex lens mounting hole and 3-9, wherein the first magnet mounting hole is arranged on the LED lamp panel; 3-10 is a first buckling hole, and 3-11 is a plano-convex mirror;

4 is a filtering component, 4-1 is a first filtering plate, 4-2 is a second filtering plate, 4-3 is a second buckling hole, 4-4 is a third magnet mounting hole, 4-5 is a first filtering plate mounting hole, 4-6 is a second shading bulge, 4-7 is a second buckle, 4-8 is a fourth magnet mounting hole, 4-9 is a second filtering plate mounting hole, and 4-10 is a third shading bulge; 4-11 is a filter;

5 is an agar pouring plate, 5-1 is a first marking hole, 5-2 is a fifth magnet mounting hole, 5-3 is a hole position mark, and 5-4 is a first agar pouring hole;

6 is a sample cell plate, 6-1 is a second marking hole, 6-2 is a seventh magnet mounting hole, 6-3 is a limiting lug, 6-4 is a sixth magnet mounting hole, and 6-5 is a limiting bulge;

7 is a glass sheet;

8 is a glass pressure ring;

9 is an agar pressing plate, 9-1 is a pressing plate, 9-2 is a handle, and 9-3 is a pressing bulge;

and 10 is a sample fixing disc.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the embodiments of the present invention, it should be noted that if the terms "upper", "lower", "horizontal", "inner", etc. are used for indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings or the orientation or positional relationship which is usually arranged when the product of the present invention is used, the description is merely for convenience and simplicity, and the indication or suggestion that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, cannot be understood as limiting the present invention. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

Furthermore, the term "horizontal", if present, does not mean that the component is required to be absolutely horizontal, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the embodiments of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

The utility model is described in further detail below with reference to the accompanying drawings:

referring to fig. 1-3, the embodiment of the utility model discloses a microscopic sample preparation device, which comprises an LED lamp panel fixing frame 1, a light homogenizing assembly 3, a light filtering assembly 4, an agar pouring plate 5, a sample cell plate 6 and a glass pressing ring 8.

As shown in fig. 4-7, the LED lamp panel holder 1 includes an annular frame, the inner ring is provided with a plurality of protrusions facing the center of the annular frame, the circular LED lamp panel 2 is disposed on the inner bottom surface of the LED lamp panel holder 1, and the top surface of the LED lamp panel 2 abuts against the plurality of protrusions of the inner ring of the LED lamp panel holder 1; a plurality of arc-shaped buckles 1-1 extending downwards are evenly arranged at the edge of a circular ring of the LED lamp panel fixing frame 1 along the circumferential direction, and the arc-shaped buckles 1-1 are connected with the dodging assembly 3 in a buckling mode. The LED lamp panel fixing frame fixes the LED lamp panel on the first light homogenizing plate 3-1 by utilizing a buckle structure, and the light homogenizing assembly, the light filtering assembly, the agar pouring plate and the sample pool plate can be mutually fixed together through magnet attraction. The glass press ring is made of metal material which can be attracted by a magnet, and the ultrathin glass can be fixed on the sample cell plate by the attraction of the magnet on the glass press ring and the sample cell plate.

The arc-shaped buckle structures evenly distributed on the periphery of the LED lamp panel fixing frame are used for placing the LED lamp panel on the first uniform light plate 3-1 in the uniform light assembly, and the positioning holes in the LED lamp panel are matched with the positioning columns in the uniform light plate to play a role in positioning the LED lamp panel. The light distribution in the single hole is more uniform in the middle of the 7 LED lamp beads and the corresponding 7 holes. The LED lamp panel fixing frame is buckled on the light homogenizing assembly. The fixing operation of the LED lamp panel can be completed at the moment.

As shown in fig. 8-13, the light uniformizing assembly 3 is disposed below the LED lamp panel 2, and the light uniformizing assembly 3 is fastened to the LED lamp panel fixing frame 1 to clamp the LED lamp panel 2; the light homogenizing component 3 is provided with a plano-convex lens mounting hole corresponding to the position of an LED lamp bead 2-2 on the LED lamp panel 2, and a plano-convex lens 3-11 is arranged in the plano-convex lens mounting hole 3-4.

The light homogenizing assembly 3 comprises a first light homogenizing plate 3-1 and a second light homogenizing plate 3-2, and the first light homogenizing plate 3-1 is arranged above the second light homogenizing plate 3-2; the plano-convex mirror mounting holes comprise first plano-convex mirror mounting holes 3-4 arranged on the first light homogenizing plate 3-1 and second plano-convex mirror mounting holes 3-8 arranged on the second light homogenizing plate 3-2, and first shading protrusions 3-9 facing the LED lamp panel 2 are arranged at the edges of the second plano-convex mirror mounting holes 3-8; the middle of the first light homogenizing plate 3-1 is provided with 7 stepped holes, so that the plano-convex mirror can be placed in the holes. The designed second light homogenizing plate 3-2 is provided with a clamping hole and a magnet mounting hole, and the corresponding second light homogenizing plate 3-2 is provided with a round clamping buckle, so that the plano-convex mirror can be fixed in the light homogenizing assembly in a clamping and combining mode. The second light homogenizing plate 3-2 is matched with the 7 holes of the first light homogenizing plate 3-1 to be provided with shading convex structures, and the main function is to fix the internal plano-convex lens and simultaneously avoid the mutual interference of light rays among the holes. The magnet mounting holes correspond to the magnet holes on the first light homogenizing plate 3-1 one by one, and the magnets can be smoothly mounted after the two plates are buckled together.

An LED lamp panel positioning column 3-5 is arranged on the upper surface of the first light homogenizing plate 3-1, and the LED lamp panel positioning column 3-5 is matched with a positioning hole 2-1 formed in the LED lamp panel 2 and used for fixing the LED lamp panel 2; the first light homogenizing plate 3-1 is provided with a plurality of first buckle holes 3-10 and a plurality of first magnet mounting holes 3-3.

The upper surface of the second light homogenizing plate 3-2 is provided with a plurality of first buckles 3-6 matched with the first buckle holes 3-10, and the second light homogenizing plate 3-2 is provided with second magnet mounting holes 3-7 corresponding to the first magnet mounting holes 3-3. The first light homogenizing plate 3-1 and the second light homogenizing plate 3-2 are buckled through the matching of a plurality of first buckles 3-6 and first buckle holes 3-10.

As shown in fig. 14-19, the filter assembly 4 is disposed below the light uniformizing assembly 3 and is fastened to the light uniformizing assembly 3; the optical filter assembly 4 is provided with an optical filter mounting hole corresponding to the position of the plano-convex lens mounting hole, and an optical filter 4-11 is arranged in the optical filter mounting hole; the first filter plate 4-1 is provided with magnet mounting holes, buckling holes and filter mounting holes, the filters are placed in the stage hole holes, and the second filter plate 4-2 is fixed on the first filter plate 4-1 in a buckling fit mode. Wherein, the first filter plate 4-1 and the second filter plate 4-2 are both provided with shading convex structures, and the shading convex structures on the first filter plate 4-1 are matched with the second light homogenizing plate 3-2 after the shading component and the filter component are matched. The situation that light interference between hole positions possibly caused by the fact that the second light homogenizing plate 3-2 is not tightly attached to the first light filtering plate 4-1 is avoided. The light-blocking protrusions on the second filter plate 4-2 are, on the one hand, intended to avoid light interference between the holes during cooperation with the mask 1. On the other hand, the filter fixing device can also play a role in fixing the filter in the axial direction.

The filtering component 4 comprises a first filtering plate 4-1 and a second filtering plate 4-2, and the first filtering plate 4-1 is arranged above the second filtering plate 4-2; the optical filter mounting holes comprise first optical filter mounting holes 4-5 formed in the first optical filter plate 4-1 and second optical filter mounting holes 4-9 formed in the second optical filter plate 4-2; the edges of the first optical filter mounting holes 4-5 are provided with second shading bulges 4-6 facing the dodging component 3; the edges of the second filter mounting holes 4-9 are all provided with third shading bulges 4-10 facing the dodging component 3.

The first filter plate 4-1 is provided with a plurality of second buckle holes 4-3 and a plurality of third magnet mounting holes 4-4; a plurality of second buckles 4-7 matched with the second buckle holes 4-3 are arranged on the upper surface of the second filter plate 4-2, and fourth magnet mounting holes 4-8 corresponding to the third magnet mounting holes 4-4 are formed in the second filter plate 4-2; the first filter plate 4-1 and the second filter plate 4-2 are buckled through the matching of a plurality of second buckles 4-7 and the second buckle holes 4-3.

As shown in fig. 20, the agar casting plate 5 is disposed below the filter assembly 4 and is engaged with the filter assembly 4; a first agar pouring hole 5-4 corresponding to the position of the optical filter mounting hole is formed in the agar pouring plate 5; the upper surface of the agar pouring plate 5 is provided with a counter bore, and the bottom surface is provided with a bulge with the same diameter as the counter bore; the diameter of the counter bore is the same as the outer diameter of the filter component 4; the filter component 4 is embedded in a counter bore of the agar casting plate 5; a first marking hole 5-1 and a plurality of fifth magnet mounting holes 5-2 are formed in the agar pouring plate 5; hole site marks 5-3 are arranged beside each first agar pouring hole 5-4. The agar pouring plate 5 and the sample pool plate 6 are buckled through magnets arranged in the fifth magnet mounting hole 5-2 and the sixth magnet mounting hole 6-4. The main body of the utility model comprises an agar pouring plate, a sample cell plate, ultra-thin glass and a glass pressure ring. The agar casting plate is of a structure with a concave front surface and a convex back surface. (the front and back correspond to the top and bottom as shown in FIG. 1, the top is the front side, and the back is the back side.) the front side is provided with magnet mounting holes and hole site marks, the hole site marks are arranged by taking the marked holes as reference and sequencing by reverse time, and the middle hole site is No. 7 hole. And the hole site marks on the reverse side are clockwise one-to-one. The hole sites on the front surface of the sample cell plate are marked to be in one-to-one correspondence with the front surfaces of the agar pouring plates in the reverse time needle sequencing. The back of the sample cell plate is provided with an annular bulge for positioning the glass sheet and the glass fixing ring, and the glass sheet and the glass pressing ring are placed in a sliding manner.

The components are assembled by the aid of the buckle structures, the components are matched in a magnetic attraction mode, and the magnetic-attraction-type magnetic-attraction type magnetic assembly can be conveniently mounted and dismounted in the actual use process. Among the above-mentioned part, plano-convex mirror horizontally face and LED lamp pearl contact can play the effect of homodisperse to the light, and the light filter can play the filtering action, plays the filtering action to the light of the wavelength that has the interference. The structure that prevents light interference between the hole has been designed, and the porose position mark of design on agar casting plate and the sample pool board that correspond avoids in the experimentation, to the confusion of hole site.

As shown in fig. 21-23, the sample cell plate 6 is disposed below the agar casting plate 5 and is engaged with the agar casting plate 5; a second agar pouring hole corresponding to the 5-4 position of the first agar pouring hole is arranged on the sample cell plate 6; the bottom surface of the sample cell plate 6 is provided with a glass sheet 7; the upper surface of the sample cell plate 6 is provided with a counter bore, and the bottom surface is provided with an annular limiting bulge 6-5; the diameter of the counter bore is the same as the external diameter of the bulge on the bottom surface of the agar pouring plate 5; the bottom surface of the agar pouring plate 5 is convexly embedded into the counter bore of the sample pool plate 6; the sample cell plate 6 is provided with a second marking hole 6-1 corresponding to the position of the first marking hole 5-1 and a sixth magnet mounting hole 6-4 corresponding to the position of the fifth magnet mounting hole 5-2.

The side surface of the sample cell plate 6 is symmetrically provided with two limiting lugs 6-3, the limiting lugs 6-3 are provided with seventh magnet mounting holes 6-2, the seventh magnet mounting holes 6-2 correspond to the eighth magnet mounting holes 10-1 on the sample fixing disc 10 in position, and the sample cell plate 6 and the sample fixing disc 10 are buckled by magnets arranged in the seventh magnet mounting holes 6-2 and the eighth magnet mounting holes 10-1.

As shown in fig. 24 to 25, the glass press ring 8 is provided below the sample cell plate 6, and is engaged with the sample cell plate 6 to press and bond the glass piece 7 to the sample cell plate 6. The glass sheet 7 and the glass pressing ring 8 are arranged in the limiting bulges 6-5 at the bottom of the sample cell plate 6, the diameter of the glass sheet 7 is the same as the outer diameter of the glass pressing ring 8, and the glass pressing ring 8 compacts the glass sheet 7 on the bottom surface of the sample cell plate 6 through the magnet in the seventh magnet mounting hole 6-2. The glass sheet 7 of the present invention is an ultra-thin glass sheet.

The embodiment of the utility model also discloses a using method of the microscopic sample preparation device, which comprises the following steps:

step 1, placing a glass sheet 7 on the back of an agar casting plate 5, fixing the glass sheet by a glass pressing ring 8 in a magnetic attraction mode, and then placing the glass sheet on a flat ultra-clean workbench surface.

And 2, respectively injecting liquid agar into 7 single holes by using a pipette gun, wherein the agar is added into the single holes according to the standard that the whole hole is basically filled with the agar.

And 3, waiting until the agar in the hole is cooled and solidified, and turning over the agar pouring plate to enable the back face to face upwards. At this time, the glass press ring can be removed, and then the glass sheet is pressed by hand to horizontally push the glass sheet in the radial direction. Because the agar is basically composed of water, the agar surface and the glass surface are jointed to play a role in lubrication, and the glass sheet is easy to remove. Thus, 7 independent cylindrical agar blocks with a flat surface were obtained.

And 4, placing the agar in a super clean bench and then airing the agar, and after the agar basically meets the requirements, distributing and dripping prepared bacteria liquid on 7 agar blocks by using a pipette. The surface of the bacterium dripping liquid is the back surface of the agar pouring plate, namely the surface for removing the glass. After the bacterial liquid is dripped, the liquid is also placed in a super clean bench to be dried, and finally tabletting operation is carried out after the requirement is met.

And step 5, as shown in FIGS. 26 to 27, assembling the sample cell plate assembly of the ultrathin glass sheet, wherein the ultrathin glass sheet is fixed at the bottom of the sample cell plate by attracting the glass press ring by the magnet on the sample cell plate. The fixed sample pool plate component and the hole position of the agar pouring plate on which the bacterial liquid is dripped are correspondingly attached together, and the whole device is placed on a smooth working table. And pressing the cylindrical agar blocks of 7 hole sites of the agar pouring plate into the hole sites of the sample cell plate by using the prepared 7-hole pressing plate. Thus, the agar block with the bacteria liquid is tightly attached to the lower glass sheet to complete the whole sample preparation process.

As shown in figure 28, the agar pressing plate 9 comprises a pressing plate 9-1, a handle 9-2 is arranged on the upper surface of the pressing plate 9-1, and a plurality of pressing bulges 9-3 corresponding to the positions of the first agar pouring holes 5-4 are arranged on the bottom surface. The pressing bulge 9-3 is 7 cylinders corresponding to the hole positions of the agar pouring plate positioned below, and can complete the tabletting operation of 7 holes at one time.

As shown in FIG. 29, the diameter φ 3 of the pressing projection 9-3 is smaller than the diameter φ 2 of the first agar pouring hole 5-4, and the diameter φ 2 of the first agar pouring hole 5-4 is smaller than the diameter φ 1 of the second agar pouring hole; the relationship thus set is to ensure that the effect of the pellet is satisfactory, and if φ 1 is set to φ 2, the cylindrical agar block in the agar casting plate is pressed into the well of the cuvette plate. Because the diameter of the agar block is equal to the diameter of the hole site, the agar block may be squeezed by the hole wall around the sample cell plate. Agar, as an elastomer, may tend to bulge in the middle, resulting in the inability of the underside of the agar block to adhere tightly to the glass plate and the inability to achieve the desired tabletting effect.

As shown in FIG. 30, the height L3 of the pressing protrusion 9-3 is greater than the hole depth L1 of the second agar pouring hole, and the hole depth L1 of the second agar pouring hole is greater than the hole depth L2 of the first agar pouring hole 5-4, so that the agar block can be well attached to the glass surface. The depth L2 of the first agar pour hole 5-4 is the same as the height L4 of the agar column.

Step 6, as shown in fig. 31, the prepared sample is placed on the sample fixing plate 10 of the laser scanning microscope. As shown in fig. 32, the middle of the sample fixing disk 10 is a through hole, and magnets are disposed on both sides of the through hole, and are matched with the magnets in the limiting lugs 6-3 on both sides of the sample cell plate 6, so as to fix the sample cell plate 6 and the sample fixing disk 10 by magnetic attraction.

The sample fixing disk 10 is a sample fixing disk on an Olympus laser scanning microscope, belongs to the existing product, and is characterized in that a magnet is arranged in a middle through hole and recesses at two sides. The structural characteristics of the sample preparation device depend on the design of the sample fixing disc, and the magnets arranged in the ear structures at two sides of the sample pool can be well attracted with the magnets in the upper drawing, so that the whole device is fixed on the fixing disc. The lens below is tightly attached to the glass sheet, and the observation of the pressed microorganisms can be completed.

After the installation is completed, the test method is used in the actual experimental process. The LED lamp plate passes through program control, can provide the light of different wavelength, and can adjust light intensity. The light rays between each single hole are independent and do not interfere with each other, and the requirements of optogenetics experiments under different illumination conditions are met.

The utility model has the following advantages:

the utility model can complete sample preparation operation of 7 samples at one time, meets the experimental requirement of medium flux, and improves the working efficiency of experimenters compared with single sample preparation at one time. The 7 holes are designed independently and are arranged on the same board. In the experiment of researching optogenetics, the illumination condition can be adjusted, and real-time comparison among multiple samples and single variables can be well realized. The structure of the utility model is adapted to a laser scanning microscope commonly used in life science research, and can carry out real-time and continuous microscope observation on samples. All the components of the device are matched in a magnetic attraction mode, and the sample preparation process of the whole device is simple and quick. The actual operation is easy to operate.

The size of the utility model is designed according to a sample fixing disc above a laser scanning microscope of Olympus, which is equivalent to a customized design. The number and arrangement mode of the holes are limited by the size, so that the holes are designed to be 7 holes, one in the middle and 6 in the periphery in circumferential arrangement. In actual design, the design can be customized according to the microscope specification of a laboratory. The number of holes and the hole site design are not necessarily determined in the above device, and are designed according to the reasonable layout among the holes. The fixing mode of the whole device and the microscope stage can also be designed according to the microscope stage which is actually used. In the above design, the glass press ring can be removed, and the glass is directly adhered to the lower surface by glue, so that the purpose of fixing the glass sheet can be achieved.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microsampling device, comprising:

the LED lamp panel fixing frame (1) comprises an annular frame, the inner ring is provided with a plurality of bulges facing the circle center of the ring, the round LED lamp panel (2) is arranged on the inner bottom surface of the LED lamp panel fixing frame (1), and the top surface of the LED lamp panel (2) is abutted against the bulges of the inner ring of the LED lamp panel fixing frame (1);

the light homogenizing assembly (3) is arranged below the LED lamp panel (2), and the light homogenizing assembly (3) is buckled with the LED lamp panel fixing frame (1) to clamp the LED lamp panel (2); a plano-convex lens mounting hole corresponding to the position of an LED lamp bead (2-2) on the LED lamp panel (2) is formed in the light homogenizing assembly (3), and a plano-convex lens (3-11) is arranged in the plano-convex lens mounting hole (3-4);

the light filtering component (4) is arranged below the light homogenizing component (3), and the light filtering component (4) is buckled with the light homogenizing component (3); the light filtering component (4) is provided with a light filter mounting hole corresponding to the position of the plano-convex lens mounting hole, and a light filter (4-11) is arranged in the light filter mounting hole;

the agar pouring plate (5) is arranged below the light filtering component (4), and the agar pouring plate (5) is buckled with the light filtering component (4); a first agar pouring hole (5-4) corresponding to the position of the optical filter mounting hole is formed in the agar pouring plate (5);

the sample pool plate (6) is arranged below the agar pouring plate (5), and the sample pool plate (6) is buckled with the agar pouring plate (5); a second agar pouring hole corresponding to the first agar pouring hole (5-4) is formed in the sample pool plate (6); a glass sheet (7) is arranged on the bottom surface of the sample cell plate (6);

glass clamping ring (8), glass clamping ring (8) set up in sample cell board (6) below, and with sample cell board (6) looks lock, compress tightly the laminating with glass piece (7) and sample cell board (6).

2. The microscopical appearance device of claim 1, characterized in that, a plurality of arc buckles (1-1) that stretch out downwards are evenly provided along circumference at the ring edge of LED lamp plate mount (1), arc buckle (1-1) and even light subassembly (3) lock connection.

3. The microsyrinthine device of claim 1, wherein the light uniformizing member (3) comprises a first light uniformizing plate (3-1) and a second light uniformizing plate (3-2), the first light uniformizing plate (3-1) being disposed above the second light uniformizing plate (3-2); the plano-convex mirror mounting holes comprise first plano-convex mirror mounting holes (3-4) formed in the first light homogenizing plate (3-1) and second plano-convex mirror mounting holes (3-8) formed in the second light homogenizing plate (3-2), and first shading protrusions (3-9) facing the LED lamp panel (2) are arranged at the edges of the second plano-convex mirror mounting holes (3-8);

an LED lamp panel positioning column (3-5) is arranged on the upper surface of the first light homogenizing plate (3-1), and the LED lamp panel positioning column (3-5) is matched with a positioning hole (2-1) formed in the LED lamp panel (2) and used for fixing the LED lamp panel (2); a plurality of first buckle holes (3-10) and a plurality of first magnet mounting holes (3-3) are formed in the first light homogenizing plate (3-1);

a plurality of first buckles (3-6) matched with the first buckle holes (3-10) are arranged on the upper surface of the second light homogenizing plate (3-2), and second magnet mounting holes (3-7) corresponding to the first magnet mounting holes (3-3) are formed in the second light homogenizing plate (3-2); the first light homogenizing plate (3-1) and the second light homogenizing plate (3-2) are buckled through the matching of a plurality of first buckles (3-6) and first buckle holes (3-10).

4. The microsampling device of claim 1, wherein said filter assembly (4) comprises a first filter plate (4-1) and a second filter plate (4-2), said first filter plate (4-1) being disposed above said second filter plate (4-2); the optical filter mounting holes comprise first optical filter mounting holes (4-5) formed in the first optical filter plate (4-1) and second optical filter mounting holes (4-9) formed in the second optical filter plate (4-2); second shading bulges (4-6) facing the dodging assembly (3) are arranged at the edges of the first optical filter mounting holes (4-5); third shading bulges (4-10) facing the dodging assembly (3) are arranged at the edges of the second optical filter mounting holes (4-9);

a plurality of second buckling holes (4-3) and a plurality of third magnet mounting holes (4-4) are formed in the first light filter plate (4-1);

a plurality of second buckles (4-7) matched with the second buckle holes (4-3) are arranged on the upper surface of the second filter plate (4-2), and fourth magnet mounting holes (4-8) corresponding to the third magnet mounting holes (4-4) are formed in the second filter plate (4-2); the first filter plate (4-1) and the second filter plate (4-2) are buckled through the matching of a plurality of second buckles (4-7) and second buckle holes (4-3).

5. The microscopical sample preparation device of claim 1, wherein the upper surface of the agar pouring plate (5) is provided with a counter bore, and the bottom surface is provided with a bulge with the same diameter as the counter bore; the diameter of the counter bore is the same as the outer diameter of the filtering component (4); the light filtering component (4) is embedded into a counter bore of the agar pouring plate (5); a first marking hole (5-1) and a plurality of fifth magnet mounting holes (5-2) are formed in the agar pouring plate (5); hole site marks (5-3) are arranged beside each first agar pouring hole (5-4).

6. The microscopical sample preparation device of claim 5, characterized in that, the upper surface of the sample cell plate (6) is provided with a counter bore, and the bottom surface is provided with an annular limiting bulge (6-5); the diameter of the counter bore is the same as the outer diameter of the protrusion on the bottom surface of the agar pouring plate (5); the bottom surface of the agar pouring plate (5) is convexly embedded into a counter bore of the sample pool plate (6); a second marking hole (6-1) corresponding to the first marking hole (5-1) and a sixth magnet mounting hole (6-4) corresponding to the fifth magnet mounting hole (5-2) are formed in the sample cell plate (6);

the agar pouring plate (5) and the sample pool plate (6) are buckled through magnets arranged in the fifth magnet mounting hole (5-2) and the sixth magnet mounting hole (6-4).

7. The microscopical sample preparation device of claim 1 or 6, characterized in that, two limit lugs (6-3) are symmetrically arranged on the side of the sample cell plate (6), a seventh magnet mounting hole (6-2) is arranged on the limit lugs (6-3), the seventh magnet mounting hole (6-2) corresponds to the eighth magnet mounting hole (10-1) on the sample fixing disk (10), and the sample cell plate (6) and the sample fixing disk (10) are buckled by the magnets arranged in the seventh magnet mounting hole (6-2) and the eighth magnet mounting hole (10-1).

8. The micro-sample preparation device according to claim 6, wherein the glass sheet (7) and the glass press ring (8) are both arranged in a limiting bulge (6-5) at the bottom of the sample cell plate (6), the diameter of the glass sheet (7) is the same as the outer diameter of the glass press ring (8), and the glass press ring (8) presses the glass sheet (7) on the bottom surface of the sample cell plate (6) through the magnet in the seventh magnet mounting hole (6-2).