CN211123467U

CN211123467U - Phase contrast microscope multi-mode ultra-thin light source device

Info

Publication number: CN211123467U
Application number: CN201922365389.0U
Authority: CN
Inventors: 范永涛; 罗海清; 孙彦华; 李�杰
Original assignee: Shanghai Guanna Intelligent Technology Co ltd
Priority date: 2019-12-25
Filing date: 2019-12-25
Publication date: 2020-07-28
Anticipated expiration: 2029-12-25

Abstract

The utility model discloses a phase contrast microscope's ultra-thin light source device of multi-mode, including L ED array, L ED base, transparent light guide ring, diffusion sheet and glass, L ED array welding fix on L ED base, the pot head of transparent light guide ring is in the well annular array inside and outside of L ED array, the other end links to each other with diffusion sheet, diffusion sheet carries out homogenization treatment with the light that L ED lamp sent, glass hugs closely in the diffusion sheet outside, be used for fixed diffusion sheet and transparent light guide ring this light source device passes through the circuit switch, can realize the switching of two kinds of imaging mode of phase contrast illumination and light field illumination, and the overall height is less than 20mm, can be applied to in the higher microscope of degree of automation requirement very conveniently.

Description

Phase contrast microscope multi-mode ultra-thin light source device

Technical Field

The utility model relates to a part of a phase contrast microscope, in particular to a light source device of the phase contrast microscope.

Background

Phase contrast microscopy is the most commonly used microscope in biological studies, which often involves the observation of thin, transparent samples, such as cells, which are often difficult to directly see under ordinary light microscopy because they are too thin and transparent. When we observe cells in middle school biology experiments, we often use a specific dye to stain the cells, or directly observe colored cells, such as onion epidermal cells. However, in actual biological research, a method for directly observing cells without treatment is needed, and the phase contrast microscope well solves the problem. After the illumination light passes through the transparent sample, the imaging cannot be directly observed due to small light intensity change, but due to the components and the density of the sample, different phase shifts are generated when the light passes through, and the phase contrast microscope essentially performs imaging by using the phase shift. The good illumination condition is an indispensable condition for biological research, in order to provide the good illumination condition, the light source devices of the existing phase contrast microscopes are complex and have large volume, and in addition, the light path needs to be processed when switching between phase contrast imaging and bright field imaging, so the operation is very inconvenient.

In the prior art, the invention patent "light source device of fluorescence microscope" (patent No. CN105445919A) discloses a light source device of fluorescence microscope, which is in a circular rotating disk shape, and more than two L EDs are distributed on the rotating disk, and the switching between different L EDs is realized by rotating the rotating disk, the invention patent "integrated light source device for microscope objective array" (patent No. CN 110244443A) discloses an integrated light source device for microscope objective array, which is more uniform.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a phase contrast microscope's ultra-thin light source device of multi-mode to solve the not enough of the current light source device that proposes in the above-mentioned background art. The light source device has the advantages of simple structure, ultra-thin and multi-mode switching.

In order to achieve the above object, the technical solution of the present invention is as follows:

a phase contrast microscope multimode ultrathin light source device comprises an L ED base, wherein a L ED array is installed on a L ED base, the L ED array comprises an inner circular array, a middle annular array is surrounded outside the inner circular array, an outer annular array is surrounded outside the middle annular array, annular light shielding plates are fixed on the inner side and the outer side of the middle annular array, a light diffusion plate is installed below the light diffusion plate, and glass is installed below the light diffusion plate.

In a further improvement, the annular light shading plate is a transparent light guide ring, and black paint is sprayed on the inner wall and the outer wall of the transparent light guide ring (3).

In a further improvement, the L ED array is composed of L ED lamps in a series of concentric circles, and the inner circular array, the middle circular array and the outer circular array are all arranged concentrically.

The improved structure comprises a third ring array, wherein the middle ring array is an L ED array, and transparent light guide rings are embedded on the inner side and the outer side of the third ring array.

The light diffusion plate is closely connected with the lower end of the transparent light guide ring to prevent light from diffusing outwards, and the light diffusion plate performs homogenization treatment on light emitted by the L ED lamp.

In a further improvement, the glass is closely adhered to the light diffusion plate to fix the light diffusion plate.

In a further improvement, the center of the L ED array is located on the same optical axis as the center line of the objective lens

In a further improvement, the annular light shielding plate is arranged obliquely relatively to form a light cavity with a wide upper part and a narrow lower part.

In a further improvement, a perforated plate is mounted below the glass, and an objective lens is mounted below the perforated plate.

The use method of the multimode ultrathin light source device of the phase contrast microscope comprises the following steps:

step one, when phase contrast imaging is required, lightening a middle annular array in a transparent light guide ring;

step two, when bright field imaging is required, all L ED arrays are lighted.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) the structure is simple and ultrathin, and the light source device is only 2cm thick.

(2) The multimode switching realizes the conversion between the phase contrast imaging and the bright field imaging by controlling the switch without adding any optical element in an imaging light path during the bright field imaging and the phase contrast imaging.

(3) When phase contrast imaging is carried out, the transparent light guide ring can ensure the illumination range, so that the illumination area is more accurate, and the measurement is more accurate.

Drawings

FIG. 1 is a schematic diagram of an embodiment of the phase contrast microscope multimode ultra-thin light source device of the present invention;

FIG. 2 is a schematic structural diagram of L ED array, L ED base and transparent light guide ring in the phase contrast microscope multimode ultra-thin light source device of the present invention;

fig. 3 is a schematic circuit diagram of the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1-L ED base, 2-L ED array, 21-inner circular array, 22-middle circular array, 23-outer circular array, 3-transparent light guide ring, 4-light diffusion plate, 5-glass, 6-porous plate, 7-objective lens, 8-optical cavity, 9-first switch and 10-second switch.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

Referring to fig. 1, the present invention provides a multi-mode ultra-thin light source device for a phase-contrast microscope, comprising L ED base 1, L ED array 2, transparent light-guiding ring 3, light-diffusing plate 4 and glass 5, wherein the L ED base 1 is circular, the L ED base 1 is annularly distributed with round holes, so that the L ED array 2 is just embedded therein, one end of the transparent light-guiding ring 3 is sleeved on the inner and outer sides of the middle annular array 22, the other end of the transparent light-guiding ring 3 is tightly adhered to the light-diffusing plate 4, and the light-diffusing plate 4 is connected to the glass 5.

The center of the L ED array 2 is located on the same optical axis as the center line of the objective lens 7.

The first embodiment is as follows: phase contrast imaging

Firstly, a phase contrast imaging mode is selected, then the middle ring array 22 of the L ED array 2 in the circle where the transparent light guide ring 3 is located is lightened, the transparent light guide ring 2 can effectively prevent light emitted by the middle ring array 22 of the L ED array 2 from diffusing outwards, light of the light becomes more uniform after passing through the light diffusion plate 4, the light irradiates on an object in the porous plate 6 after passing through the glass 5, and the object is amplified by the objective lens 7 to form phase contrast imaging.

Second embodiment bright field imaging

Firstly, a bright field imaging mode is selected, then the whole L ED array 2 is lightened, the light emitted by the L ED array 2 is more uniform after passing through the light diffusion plate 4, and is irradiated on an object in the porous plate 6 after passing through the glass 5, and the object is magnified by the objective lens 7 to form a bright field image.

The circuit diagram of the present invention, i.e. the L ED lamps on the inner circular array 21 and the outer circular array 23 are arranged in parallel with the L ED lamps on the middle circular array 22, and the L ED lamps of the inner circular array 21 and the outer circular array 23 are electrically connected with the first switch 9, and the L ED lamps on the middle circular array 22 are electrically connected with the second switch 10.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A multimode ultrathin light source device of a phase-contrast microscope is characterized by comprising an L ED base (1), wherein a L ED array (2) is installed on a L ED base (1), the L ED array (2) comprises an inner circular array (21), a middle circular array (22) is surrounded outside the inner circular array (21), an outer circular array (23) is surrounded outside the middle circular array (22), annular light shielding plates are fixed on the inner side and the outer side of the middle circular array (22), a light diffusion plate (4) is installed below the middle circular array (22), and glass (5) is installed below the light diffusion plate (4).

2. The phase-contrast microscope multimode ultrathin light source device as claimed in claim 1, characterized in that the annular light shielding plate is a transparent light guide ring (3), and black paint is sprayed on the inner wall and the outer wall of the transparent light guide ring (3).

3. The phase-contrast microscope multimode ultrathin light source device as claimed in claim 2, characterized in that the L ED array (2) is composed of L ED lamps with a series of concentric circles, and the inner circular array (21), the middle circular array (22) and the outer circular array (23) are all concentrically arranged.

4. The phase-contrast microscope multimode ultrathin light source device according to claim 3 is characterized in that the middle ring array (22) is a third ring array of L ED arrays (2), and the transparent light guide rings (3) are embedded on the inner side and the outer side of the third ring array.

5. The phase-contrast microscope multimode ultrathin light source device according to claim 1, characterized in that the light-diffusing plate (4) is closely connected with the lower end of the transparent light-guiding ring (3) to prevent light from diffusing outwards.

6. The multimode ultra-thin light source device of phase contrast microscope according to claim 1, wherein the glass (5) is closely attached to the light-diffusing plate (4) to fix the light-diffusing plate (4).

7. The multimode ultrathin light source device of the phase contrast microscope as claimed in claim 1, characterized in that the center of the L ED array (2) is located on the same optical axis as the center line of the objective lens (7).

8. The phase-contrast microscope multimode ultra-thin light source device of claim 1, characterized in that: the annular light shielding plates are arranged oppositely in an inclined mode to form a light cavity (8) which is wide at the top and narrow at the bottom.

9. The phase-contrast microscope multimode ultra-thin light source device of claim 1, characterized in that: a porous plate (6) is installed below the glass (5), and an objective lens (7) is installed below the porous plate (6).