CN216663111U

CN216663111U - Conversion extraction element

Info

Publication number: CN216663111U
Application number: CN202123377726.1U
Authority: CN
Inventors: 马祥亮; 刘树蓬; 刘磊
Original assignee: Bayannur Huaheng Biotechnology Co ltd; Hefei Huaheng Biological Engineering Co ltd; Anhui Huaheng Biotechnology Co Ltd
Current assignee: Bayannur Huaheng Biotechnology Co ltd; Hefei Huaheng Biological Engineering Co ltd; Anhui Huaheng Biotechnology Co Ltd
Priority date: 2021-12-30
Filing date: 2021-12-30
Publication date: 2022-06-03
Anticipated expiration: 2031-12-30

Abstract

The utility model relates to a conversion extraction device, comprising: a dosing assembly for holding a substrate solution; the reaction component is communicated with the batching component and is suitable for mixing the substrate solution with a target medium to obtain a target solution; the filtering component is communicated with the reaction component and is suitable for filtering and removing impurities from the target solution conveyed by the reaction component to obtain the target solution; the concentration and centrifugation component is communicated with the filtering component and is suitable for carrying out concentration crystallization and centrifugation treatment on the target solution to obtain target crystals; wherein, the reaction assembly comprises at least two reaction pieces, and the at least two reaction pieces are in switchable communication with the ingredient assembly and the filtering assembly. The reaction component is provided with at least two reaction parts, so that the batching component can carry out conversion reaction through at least one reaction part, thereby realizing continuous production of DL-alanine products and improving the production efficiency; still through setting up the effective edulcoration of multistage filtering component, improved the purity of target product.

Description

Conversion extraction element

Technical Field

The utility model relates to a conversion extraction device, and belongs to the field of production of DL-alanine by enzyme conversion and extraction thereof.

Background

DL-alanine is chemically named as DL-alpha-aminopropionic acid and has a molecular formula of CH₃CH(NH₂) COOH, molecular weight 89.09, colorless to white odorless needle crystal or crystalline powder, sweet, easily soluble in water, slightly soluble in ethanol, insoluble in diethyl ether and acetone, and no optical activity. DL-alanine is mainly used as food additive and is also an important medical intermediate. Has been widely used in food industry abroad, and has great annual demand and rapid growth.

At present, the production process of DL-alanine at home and abroad mainly comprises a chemical synthesis method, a direct fermentation method and an enzyme method, wherein the chemical synthesis method comprises a Strecker method, a Buchere method, an alpha-halogenated carboxylic acid ammoniation method, a phase transfer catalytic synthesis method and a chemical racemization method. DL-alanine obtained by the chemical production process often has a series of problems of difficult separation from byproducts, large catalyst consumption, environmental pollution, high cost and the like. In the fermentation method, the saccharic acid conversion rate is low, the impurity composition of the fermentation liquid is complex, and the extraction cost is high from the fermentation level reported at present. The production process of the alanine racemase method is a one-step biochemical reaction with L-alanine as a raw material and racemase as a catalyst, and has the advantages of simple extraction process, mild reaction conditions, high enzyme activity, high reaction speed and high yield which is generally more than 95%. Under the current price of L-alanine, the alanine racemase production process has obvious cost advantage. However, the DL-alanine produced by the alanine racemase method at present mostly adopts a 'one-pot' production process, the degree of continuity is not high, and the production efficiency is low.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a conversion extraction device, which can realize continuous production of DL-alanine products and improve the production efficiency.

In order to achieve the purpose, the utility model provides the following technical scheme: a conversion extraction apparatus comprising: a dosing assembly for holding a substrate solution; a reaction component, communicated with the batching component, and suitable for mixing the substrate solution with a target medium to obtain a target solution; the filtering component is communicated with the reaction component and is suitable for filtering and removing impurities from the target solution conveyed by the reaction component; the concentration and centrifugation component is communicated with the filtering component and is suitable for concentrating and centrifuging the target solution subjected to impurity removal to obtain target crystals; wherein the reaction assembly comprises at least two reaction members in switchable communication with the filter assembly.

Further, the reaction assembly further comprises a first valve body and a second valve body which are respectively communicated with at least two of the reaction members, and the batching assembly can be selectively communicated with the two reaction members through the first valve body and/or the second valve body respectively.

Further, the filter assembly includes a first filter element in communication with the reaction assembly, the first filter element adapted to filter insoluble impurities within the target solution within the reaction assembly.

Further, the filter assembly further comprises a decoloring tank communicated with the first filter element, a second filter element is arranged in the decoloring tank, and the target solution is mixed and decolored with the second filter element in the decoloring tank.

Further, the second filter element is activated carbon.

Further, the filter assembly further includes a third filter element in communication with the decolorizing tank, the third filter element adapted to separate the activated carbon from the target solution.

Further, the concentration centrifugal assembly comprises a concentration tank communicated with the third filtering element, and the concentration tank is suitable for carrying out vacuum concentration and temperature reduction crystallization on the target solution to obtain a crystal solution.

Further, the concentration centrifugal assembly also comprises a centrifugal device communicated with the concentration tank, and the centrifugal device is suitable for carrying out centrifugal treatment on the crystal solution to realize solid-liquid separation.

Further, the conversion and extraction device also comprises a drying component connected with the centrifugal equipment.

Further, conversion extraction element still includes driving piece and agitator, the driving piece drive the agitator is rotatory, the agitator sets up on batching subassembly and decoloration jar for the stirring of jar interior solution is mixed.

The utility model has the beneficial effects that: at least two reaction pieces are arranged in the reaction assembly, the at least two reaction pieces are in switchable communication with the batching assembly and the filtering assembly, a substrate solution in the batching assembly can be switched to react with a target medium in any one or more reaction pieces to generate a target solution, other reaction pieces are used as spare reaction pieces, and when the enzyme activity of the target medium in the used reaction piece, such as immobilized enzyme, is reduced, the spare reaction pieces can be switched to use, so that the continuous production of DL-alanine products is realized, and the production efficiency is improved; still through setting up the effective edulcoration of multistage filtering component, improved the purity of target product.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.

Drawings

FIG. 1 is a schematic structural diagram of a reforming extraction apparatus according to the present invention.

Description of reference numerals: the device comprises a dosing assembly 1, a reaction assembly 2, a reaction member 21, a first valve body 22, a second valve body 23, a filtering assembly 3, a first filtering piece 31, a buffer tank 32, a decoloring tank 33, a third filtering piece 34, a concentration centrifugal assembly 4, a concentration tank 41, a centrifugal device 42, a drying assembly 5, a first driving piece 6, a second driving piece 7, a third driving piece 8, a first stirrer 9, a second stirrer 10 and a third stirrer 11.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. The utility model will be described in detail hereinafter with reference to the accompanying drawings in conjunction with embodiments. It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.

It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order.

In the present invention, unless specified to the contrary, use of the terms of orientation such as "upper, lower, top, bottom" or the like, generally refer to the orientation as shown in the drawings, or to the component itself in a vertical, perpendicular, or gravitational orientation; likewise, for ease of understanding and description, "inner and outer" refer to inner and outer relative to the profile of the components themselves, but the above directional terms are not intended to limit the utility model.

Referring to FIG. 1, a transformation and extraction apparatus for transformation and extraction of DL-alanine product according to a preferred embodiment of the present invention is shown. In other embodiments, the conversion and extraction device can also be used for converting and extracting related products of other enzymes, and the usage scenario is not particularly limited herein.

The conversion extraction device comprises a material distribution component 1, a reaction component 2 communicated with the material distribution component 1, a filtering component 3 communicated with the reaction component 2 and a concentration centrifugal component 4 communicated with the filtering component 3. The material preparing component 1 is used for storing a substrate solution, the reaction component 2 is suitable for mixing the substrate solution with a target medium to obtain a target solution, the filtering component 3 is suitable for filtering the target solution conveyed by the reaction component 2 to obtain a target solution without impurities, the target solution without impurities is concentrated and centrifuged by the concentrating and centrifuging component 4 to obtain target crystals, and the target crystals are leached and dried to obtain a final product. In this example, the substrate solution was an aqueous solution of L-alanine and pure water. And the drying treatment is realized by a drying component 5 connected with the concentration centrifugal component 4, and the drying component 5 can be one or more of a drying box, a dryer and a fluidized bed drying device. In other embodiments, other drying equipment may be used for the drying process. Specifically, the reaction assembly 2 comprises at least two reaction members 21 communicating the dosing assembly 1 and the filtering assembly 3, and at least two first valve bodies 22 and second valve bodies 23 communicating with the two reaction members 21, respectively. Two of the reaction members 21 are provided with immobilized enzymes for carrying out racemase conversion of the substrate solution, and a target solution containing DL-alanine is obtained under the enzyme catalysis of the immobilized enzymes. It should be noted that, in this embodiment, the dispensing component 1 may selectively communicate with the two reaction parts 21 through the first valve body 22 and/or the second valve body 23, respectively, and then the substrate solution reacts with the immobilized enzyme in any one of the reaction parts 21 to generate a target solution containing DL-alanine; for example, when the substrate solution reacts with the immobilized enzyme in the left reaction member 21, the right reaction member 21 is ready for use, and when the enzyme activity of the immobilized enzyme in the left reaction member 21 is reduced, the right reaction member 21 is switched to perform the enzyme-catalyzed reaction, so that the continuous production of the DL-alanine product is realized, and the production efficiency is improved. The filter assembly 3 comprises a first filter element 31 communicating with the reaction assembly 2, the first filter element 31 being adapted to filter insoluble impurities of the target solution in the reaction assembly 2 to obtain a target solution clear liquid. In this embodiment, the first filter member 31 is a bag filter.

And pump bodies are arranged on pipelines between the dosing assembly 1 and the reaction assembly 2 and between the reaction assembly 2 and the first filtering piece 31 and are used for conveying substrate solution or target solution containing DL-alanine.

The filter assembly 3 further includes a buffer tank 32 communicating with the first filter member 31 and a decolorizing tank 33 communicating with the buffer tank 32. The buffer tank 32 is used for storing the clear target solution filtered by the first filter 31, and a pump body is arranged between the buffer tank 32 and the decolorizing tank 33 and pumps the target solution in the buffer tank 32 into the decolorizing tank 33. The second filter member is provided in the decoloring tank 33, and the target solution is mixed with the second filter member in the decoloring tank 33. In this embodiment, the second filter member is activated carbon, which can remove impurities such as pigments remaining in the target solution to perform the decoloring treatment.

The filter assembly 3 further includes a third filter 34 in communication with the decolorizing tank 33, the third filter 34 being adapted to filter the target solution mixed with the activated carbon in the decolorizing tank 33 to separate the activated carbon from the target solution. In this embodiment, the third filter 34 is a plate and frame filter.

The concentration centrifugal component 4 comprises a concentration tank 41 communicated with the third filter element 34 and a centrifugal device 42 communicated with the concentration tank 41, wherein the concentration tank 41 is used for containing the target solution filtered by the third filter element 34, carrying out vacuum concentration, cooling crystallization, centrifuging by the centrifugal device 42 to remove mother liquor to obtain a crystal sample, and drying the crystal sample by the drying component 5 to obtain the target product DL-alanine. The centrifugal device 42 is adapted to subject the crystal-containing liquid mixture to centrifugal treatment to effect solid-liquid separation to obtain the objective crystals. In this embodiment, the centrifugal device 42 may be a scraper centrifuge or a plate decanter centrifuge.

In order to make the reaction more complete, in this embodiment, the conversion extraction device further includes a driving member and a stirrer, and the driving member drives the stirrer to rotate. Specifically, in this embodiment, the conversion and extraction device includes a first driving member 6, a first stirrer 9 connected to the first driving member 6, a second driving member 7, a second stirrer 10 connected to the second driving member 7, a third driving member 8, and a third stirrer 11 connected to the third driving member 8. Wherein, first agitator 9 stretches into the setting in the batching jar of batching subassembly 1, and second agitator 10 stretches into to decoloration jar 33 in, and third agitator 11 stretches into the setting in concentrated jar 41, and first agitator 9, second agitator 10 and third agitator 11 stir the internal solution of corresponding jar respectively for the internal solution reaction of jar is abundant or prevent to deposit etc..

In summary, the working process of the converting and extracting apparatus is to dissolve DL-alanine in pure water in the batching tank to form a substrate solution, the substrate solution is conveyed into the reaction member 21 under the action of the pump body, a target solution containing DL-alanine is obtained under the catalytic action of an enzyme immobilized in the reaction member 21, the target solution is filtered by the first filtering member 31 to obtain a target solution without insoluble impurities, the target solution at this time is conveyed into the decolorizing tank 33 under the action of the pump body, and the target solution in the decolorizing tank 33 is decolorized by activated carbon to filter out impurities such as pigment and the like remaining in the target solution. Under the action of the pump body, the target solution faded by the activated carbon enters the third filter piece 34, the activated carbon and the target solution are separated by the third filter piece 34, the separated target solution enters the concentration tank 41 for vacuum concentration, after the concentration is finished, the temperature is reduced for crystallization, the mother solution is removed by centrifugation through the centrifugal equipment 42, the solid-liquid separation is realized to obtain target crystals, and finally the target crystals are leached and dried to obtain the product. In this example, the target crystal was DL-alanine.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A conversion extraction apparatus, comprising:

a dosing assembly for holding a substrate solution;

a reaction component, which is communicated with the batching component and is suitable for mixing the substrate solution with a target medium to obtain a target solution;

the filtering component is communicated with the reaction component and is suitable for filtering and removing impurities from the target solution conveyed by the reaction component;

the concentration and centrifugation component is communicated with the filtering component and is suitable for concentrating and centrifuging the target solution after impurity removal to obtain target crystals;

wherein the reaction assembly comprises at least two reaction members in switchable communication with the filter assembly.

2. The reforming extraction apparatus defined in claim 1, wherein said reaction assembly further comprises first and second valve bodies in communication with at least two of said reaction members, respectively, and said dosing assembly is selectively in communication with both of said reaction members through said first and/or second valve bodies, respectively.

3. The conversion extraction assembly according to claim 1, wherein said filter assembly includes a first filter element in communication with said reaction assembly, said first filter element adapted to filter insoluble impurities in said target solution in said reaction assembly.

4. The conversion extraction apparatus according to claim 3, wherein said filter assembly further comprises a decolorizing tank in communication with said first filter, said decolorizing tank having a second filter disposed therein, said target solution being decolorized by mixing with said second filter in said decolorizing tank.

5. The reforming extraction apparatus defined in claim 4, wherein the second filter element is activated carbon.

6. The reforming extraction apparatus defined in claim 5, wherein the filter assembly further comprises a third filter element in communication with the decolorizing tank, the third filter element adapted to separate the activated carbon from the target solution after decolorization.

7. The apparatus according to claim 6, wherein the concentration and centrifugation assembly comprises a concentration tank in communication with the third filter element, the concentration tank being adapted to perform vacuum concentration and temperature-reducing crystallization on the target solution to obtain a crystal solution.

8. The conversion extraction apparatus of claim 7, wherein the concentration centrifuge assembly further comprises a centrifuge device in communication with the concentration tank, the centrifuge device adapted to effect solid-liquid separation of the crystal solution.

9. The apparatus of claim 8, further comprising a drying assembly coupled to the centrifuge device.

10. The apparatus of claim 4, further comprising a drive member for driving rotation of the agitator and an agitator disposed on the dosing assembly and the decolorization tank for agitating and mixing the solution in the tank.