CN216646264U - Optical path frame for fluorescent detector - Google Patents
Optical path frame for fluorescent detector Download PDFInfo
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- CN216646264U CN216646264U CN202122837810.0U CN202122837810U CN216646264U CN 216646264 U CN216646264 U CN 216646264U CN 202122837810 U CN202122837810 U CN 202122837810U CN 216646264 U CN216646264 U CN 216646264U
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Abstract
The utility model relates to an optical path frame for a fluorescence detector, which comprises a base, wherein a fluorescence detection channel is arranged on the base, a photosensitive diode sensor is arranged at one end of the fluorescence detection channel, a detection diaphragm, a detection lens, a detection optical filter, a dichroic mirror and a primary lens are sequentially arranged in the fluorescence detection channel from one end where the photosensitive diode sensor is located to the other end, the mirror surface of the dichroic mirror is inclined to the cross section of the fluorescence detection channel, the included angle between the mirror surface of the dichroic mirror and the cross section of the fluorescence detection channel is 40-50 degrees, an excitation light path channel is also arranged on the base, the mirror surface of the dichroic mirror is inclined to the cross section of the excitation light path channel, the included angle between the mirror surface of the dichroic mirror and the cross section of the excitation light path channel is 40-50 degrees, and an excitation light trap is arranged at the inner end of the excitation light path channel. The light path frame for the fluorescence detector has a more accurate and reliable detection result.
Description
Technical Field
The utility model relates to an optical path frame for a fluorescence detector.
Background
Because of the characteristics of fluorescence detection, most of the energy of fluorescence emitted by the fluorescent dye comes from exciting light, but the light path frame for the existing fluorescence detector has an unreasonable structural design, so that the light signals in the exciting light channel and the fluorescence detection channel on the light path frame interfere with each other, the detection sensitivity is deteriorated, and the accuracy of a detection result is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an optical path frame for a fluorescence detector, which has more accurate and reliable detection results.
The utility model relates to an optical path frame for a fluorescence detector, which comprises a base, wherein a fluorescence detection channel which linearly penetrates through the whole base is arranged on the base, a photosensitive diode sensor is arranged at one end of the fluorescence detection channel, a detection diaphragm, a detection lens, a detection optical filter, a dichroic mirror and a primary lens are sequentially arranged in the fluorescence detection channel from one end where the photosensitive diode sensor is located to the other end, the detection diaphragm, the detection lens, the detection optical filter and the primary lens are arranged perpendicular to the fluorescence detection channel, the mirror surface of the dichroic mirror is inclined to the cross section of the fluorescence detection channel, the included angle between the mirror surface of the dichroic mirror and the cross section of the fluorescence detection channel is 40-50 degrees, an excitation optical path is also arranged on the base, the excitation optical path penetrates through the position of the dichroic mirror in the fluorescence detection channel from one end surface of the base along the perpendicular direction of the fluorescence detection channel, the mirror surface of the dichroic mirror is inclined to the cross section of the excitation light path channel, the included angle between the mirror surface of the dichroic mirror and the cross section of the excitation light path channel is 40-50 degrees, and the excitation light trap is arranged at the inner end of the excitation light path channel.
The light path frame for the fluorescence detector is characterized in that a black light absorption material is distributed in the exciting light trap.
The optical path frame for the fluorescence detector is characterized in that the detection lens and the primary lens are respectively convex lenses, an included angle between the mirror surface of the dichroic mirror and the cross section of the fluorescence detection channel is 45 degrees, and an included angle between the mirror surface of the dichroic mirror and the cross section of the excitation light path is 45 degrees.
The optical path frame for the fluorescence detector is characterized in that the cross section of the fluorescence detection channel and the cross section of the excitation light path channel are respectively circular or square.
The optical path frame for the fluorescence detector is characterized in that the fluorescence detection channel is arranged along the vertical direction, and the excitation optical path channel is arranged along the left and right horizontal directions.
The light path frame for the fluorescence detector comprises a rectangular rear base and a rectangular front base, wherein the front surface of the rear base is attached to the rear surface of the front base, and the fluorescence detection channel and the excitation light path channel are positioned between the front part of the rear base and the rear part of the front base.
The utility model relates to an optical path frame for a fluorescence detector, wherein a rear mounting plate is arranged at the rear side edge of the top end of a rear base, and the plate surface of the rear mounting plate is positioned in the left-right horizontal direction; a front mounting plate is arranged at the front side edge of the top end of the front base, and the plate surface of the front mounting plate is positioned in the left-right horizontal direction; a plurality of bolt holes are respectively formed in the plate surface of the front mounting plate and the plate surface of the rear mounting plate.
The light path frame for the fluorescence detector can directly irradiate exciting light on the dichroic mirror, the dichroic mirror has a reflecting mirror relative to the exciting light, the dichroic mirror reflects the exciting light to the primary lens, then the exciting light is focused by the primary lens and then irradiated on the fluorescent dye below, so that the fluorescent dye is excited to generate fluorescent light, the generated fluorescent light is focused by the primary lens and irradiated on the dichroic mirror, the exciting light and the fluorescent light are not in a wavelength band, so the dichroic mirror is transparent glass relative to the fluorescent light, the fluorescent light passes through the dichroic mirror, then continuously propagates along a fluorescence detection channel, passes through a detection filter, a detection lens and a detection diaphragm and then irradiates on a photosensitive diode sensor, and the photosensitive diode sensor converts the intensity of an optical signal into the intensity of an electric signal, and then transmitted to a computer for analysis processing. Therefore, the optical path frame for the fluorescence detector has the characteristic of more accurate and reliable detection result.
The optical path frame for a fluorescence detector according to the present invention will be described in further detail with reference to the accompanying drawings.
Drawings
FIG. 1 is a front sectional view schematically showing the structure of an optical path holder for a fluorescence detector according to the present invention;
FIG. 2 is a top view of FIG. 1;
fig. 3 is a side view of fig. 2.
Detailed Description
As shown in FIG. 1, FIG. 2 and FIG. 3, the optical bench for fluorescence detector of the present invention comprises a base 1, a fluorescence detection channel 2 linearly penetrating the whole base is arranged on the base 1, a photodiode sensor 3 is arranged at one end of the fluorescence detection channel 2, a detection diaphragm 4, a detection lens 5, a detection filter 6, a dichroic mirror 7 and a primary lens 8 are sequentially arranged in the fluorescence detection channel 2 from the end where the photodiode sensor 3 is located to the other end, the detection diaphragm 4, the detection lens 5, the detection filter 6 and the primary lens 8 are arranged perpendicular to the fluorescence detection channel 2, the mirror surface of the dichroic mirror 7 is inclined to the cross section of the fluorescence detection channel 2, the included angle between the mirror surface of the dichroic mirror 7 and the cross section of the fluorescence detection channel 2 is 40-50 degrees, an excitation light path 9 is further arranged on the base 1, the excitation light path 9 penetrates the dichroic mirror surface of the fluorescence detection channel 2 from one end surface of the base 1 along the perpendicular direction of the fluorescence detection channel 2 The dichroic mirror 7 is positioned, the mirror surface of the dichroic mirror 7 is inclined to the cross section of the excitation light path channel 9, the included angle between the mirror surface of the dichroic mirror 7 and the cross section of the excitation light path channel 9 is 40-50 degrees, and the excitation light trap 10 is arranged at the inner end of the excitation light path channel 9. The excitation light trap 10 functions to attenuate excitation light that is not sufficiently reflected by the processing portion, thereby reducing interference with detection of fluorescence when the excitation light is strong.
As an improvement of the present invention, a black light absorbing material is disposed in the excitation light trap 10, and the black light absorbing material can further absorb the excitation light irradiated into the excitation light trap 10, so as to reduce the interference of stray light to the detection light.
As a further improvement of the utility model, the detection lens 5 and the primary lens 8 are convex lenses, respectively, the angle between the mirror surface of the dichroic mirror 7 and the cross section of the fluorescence detection channel 2 is 45 °, and the angle between the mirror surface of the dichroic mirror 7 and the cross section of the excitation light path channel 9 is 45 °.
As a further improvement of the present invention, the cross-section of the fluorescence detection channel 2 and the cross-section of the excitation light path channel 9 are circular or square, respectively.
As a further improvement of the utility model, the fluorescence detection channel 2 is arranged along the vertical direction, and the excitation light path channel 9 is arranged along the left-right horizontal direction.
As a further improvement of the present invention, the base 1 comprises a rectangular rear base and a rectangular front base, the front surface of the rear base is attached to the rear surface of the front base, and the fluorescence detection channel 2 and the excitation light path channel 9 are located between the front part of the rear base and the rear part of the front base.
As a further improvement of the utility model, a rear mounting plate 11 is arranged at the rear side edge of the top end of the rear base, and the plate surface of the rear mounting plate 11 is positioned in the left-right horizontal direction; a front mounting plate 12 is arranged at the front side edge of the top end of the front base, and the plate surface of the front mounting plate 12 is positioned in the left-right horizontal direction; a plurality of bolt holes are respectively arranged on the plate surface of the front mounting plate 12 and the plate surface of the rear mounting plate 11.
The optical path for fluorescence detector of the present invention is used to allow the excitation light to directly irradiate the dichroic mirror 7, the dichroic mirror 7 has a mirror for the excitation light, the dichroic mirror 7 reflects the excitation light to the primary lens 8, then the excitation light is focused by the primary lens 8 and then irradiated to the fluorescent dye below, so that the fluorescent dye is excited to generate fluorescence, the generated fluorescence is focused by the primary lens 8 and irradiated to the dichroic mirror 7, because the excitation light and the fluorescence are not in a wavelength band, the dichroic mirror 7 is a transparent glass for the fluorescence, so the fluorescence passes through the dichroic mirror 7, then continuously propagates along the fluorescence detection channel 2, passes through the detection filter 6, the detection lens 5 and the detection diaphragm 4 in sequence, and then irradiates the photodiode sensor 3, the photodiode sensor 3 converts the intensity of the optical signal into the intensity of the electric signal, and transmits the electric signal to a computer for analysis. Therefore, the optical path frame for the fluorescence detector has the characteristic of more accurate and reliable detection result.
Claims (7)
1. The optical path frame for the fluorescence detector is characterized in that: comprises a base (1), a fluorescence detection channel (2) which linearly penetrates through the whole base is arranged on the base (1), one end of the fluorescence detection channel (2) is provided with a photosensitive diode sensor (3), a detection diaphragm (4), a detection lens (5), a detection optical filter (6), a dichroic mirror (7) and a primary lens (8) are sequentially arranged from one end where the photosensitive diode sensor (3) is located to the other end in the fluorescence detection channel (2), the detection diaphragm (4), the detection lens (5), the detection optical filter (6) and the primary lens (8) are perpendicular to the fluorescence detection channel (2), the mirror surface of the dichroic mirror (7) is inclined to the cross section of the fluorescence detection channel (2), the included angle between the mirror surface of the dichroic mirror (7) and the cross section of the fluorescence detection channel (2) is 40-50 degrees, and an excitation light path channel (9) is further arranged on the base (1), the excitation light path channel (9) penetrates through the position where the dichroic mirror (7) in the fluorescence detection channel (2) is located from one end face of the base (1) along the vertical direction of the fluorescence detection channel (2), the mirror surface of the dichroic mirror (7) is inclined to the cross section of the excitation light path channel (9), the included angle between the mirror surface of the dichroic mirror (7) and the cross section of the excitation light path channel (9) is 40-50 degrees, and an excitation light trap (10) is arranged at the inner end of the excitation light path channel (9).
2. The optical bench for a fluorescence detector according to claim 1, wherein: and a black light absorption material is distributed in the excitation light trap (10).
3. The optical bench for a fluorescence detector according to claim 2, wherein: the detection lens (5) and the primary lens (8) are respectively convex lenses, an included angle between the mirror surface of the dichroic mirror (7) and the cross section of the fluorescence detection channel (2) is 45 degrees, and an included angle between the mirror surface of the dichroic mirror (7) and the cross section of the excitation light path channel (9) is 45 degrees.
4. The optical bench for a fluorescence detector according to claim 3, wherein: the cross section of the fluorescence detection channel (2) and the cross section of the excitation light channel (9) are respectively circular or square.
5. The optical bench for a fluorescence detector according to claim 4, wherein: the fluorescence detection channel (2) is arranged along the vertical direction, and the excitation light path channel (9) is arranged along the left-right horizontal direction.
6. The optical bench for a fluorescence detector according to claim 5, wherein: the base (1) comprises a rectangular rear base and a rectangular front base, the front surface of the rear base is attached to the rear surface of the front base, and the fluorescence detection channel (2) and the excitation light channel (9) are located between the front portion of the rear base and the rear portion of the front base.
7. The optical bench for a fluorescence detector according to claim 6, wherein: a rear mounting plate (11) is arranged at the rear side edge of the top end of the rear base, and the plate surface of the rear mounting plate (11) is positioned in the left-right horizontal direction; a front mounting plate (12) is arranged at the front side edge of the top end of the front base, and the plate surface of the front mounting plate (12) is positioned in the left-right horizontal direction; a plurality of bolt holes are respectively arranged on the plate surface of the front mounting plate (12) and the plate surface of the rear mounting plate (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122837810.0U CN216646264U (en) | 2021-11-18 | 2021-11-18 | Optical path frame for fluorescent detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122837810.0U CN216646264U (en) | 2021-11-18 | 2021-11-18 | Optical path frame for fluorescent detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216646264U true CN216646264U (en) | 2022-05-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122837810.0U Active CN216646264U (en) | 2021-11-18 | 2021-11-18 | Optical path frame for fluorescent detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216646264U (en) |
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2021
- 2021-11-18 CN CN202122837810.0U patent/CN216646264U/en active Active
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