CN216639439U - Biochip reaction box with waste liquid bag, assembly and hybridization instrument - Google Patents
Biochip reaction box with waste liquid bag, assembly and hybridization instrument Download PDFInfo
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- CN216639439U CN216639439U CN202122353647.0U CN202122353647U CN216639439U CN 216639439 U CN216639439 U CN 216639439U CN 202122353647 U CN202122353647 U CN 202122353647U CN 216639439 U CN216639439 U CN 216639439U
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Abstract
The utility model discloses a biochip reaction box with a waste liquid bag, a component and a hybridization instrument, which comprises a card box body and a card box cover, wherein a reaction bin and the waste liquid bag are arranged in the card box body; the card box cover is arranged on the card box and seals the reaction bin and the waste liquid bag. The utility model can conveniently collect the residual waste liquid of the hybridization reaction by adding the waste liquid bag, thereby avoiding the waste liquid from being thrown into a reaction bin of the instrument and ensuring the cleanness of the detection instrument and the unaffected surrounding environment/air.
Description
Technical Field
The utility model relates to the technical field of hybridization instruments, in particular to a biochip reaction box with a waste liquid bag, a component and a hybridization instrument.
Background
The traditional microarray biochip analysis system generally comprises the links of a chip hybridization instrument, cleaning, dyeing, detection and the like, wherein the links are manually completed by operators, so that the time and the labor are consumed, experimental errors can be brought by manual operation, and the possibility of pollution exists. Aiming at the problem, scientific researchers develop various microarray chip structures of self-closed hybridization instrument cleaning systems, and complete a full-automatic hybridization cleaning and dyeing integrated system, thereby realizing full automation of operation. However, current systems of this type often connect the modules by tubing, allowing different reaction or cleaning fluids to enter the cartridge through the tubing. The time taken for the liquid to enter and exit the cartridge through the tubing is relatively long, resulting in an extended total time for detection. The possibility of carrying pollution exists in the process of pipeline transmission; if the pipeline is not properly selected, the problems of pipeline aging and oil leakage are easy to occur. In order to solve the above problems, patent document CN108642142A discloses a cartridge assembly and a hybridization instrument, which have advantages such as simple structure and easy use, but waste liquid after reaction is easily thrown into a reaction chamber of the instrument unintentionally, thereby polluting the air environment around the instrument.
SUMMERY OF THE UTILITY MODEL
The utility model provides a biochip reaction box with a waste liquid bag, a component and a hybridization instrument, and aims to solve the technical problems that: the technical problem that the waste liquid thrown out from the existing biochip reaction box easily pollutes the surrounding environment.
In view of the above problems of the prior art, according to one aspect of the present disclosure, the following technical solutions are adopted in the present invention:
a biochip reaction cartridge with a waste liquid pocket, comprising:
the waste liquid collecting device comprises a card box, a reaction bin, a waste liquid bag and a flow guide groove, wherein the card box is internally provided with the reaction bin and the waste liquid bag, the flow guide groove is connected with the reaction bin and the waste liquid bag and serves as a waste liquid channel thrown out of the reaction bin, and the waste liquid bag is used for collecting waste liquid thrown out of the flow guide groove;
the card box cover is arranged on the card box and seals the reaction bin and the waste liquid bag.
In order to better realize the utility model, the further technical scheme is as follows:
furthermore, the diversion trench is a conical diversion trench, and the larger end of the diversion trench is connected with the reaction bin.
Furthermore, an inwards concave image acquisition window is arranged on the card box cover above the reaction bin.
Furthermore, a sample adding port communicated with the reaction bin is arranged on the cassette cover.
Further, the cartridge cover is provided with an exhaust hole communicated with the waste liquid bag.
Further, a film chip is arranged at the bottom in the reaction bin.
Furthermore, a pressing sheet which is clamped into the reaction bin and presses the membrane chip is arranged on the card box cover.
Furthermore, cassette cover one end sets up and is used for the card to go into the first buckle of cassette, the cassette cover other end sets up and is used for the card to go into the second buckle of waste liquid bag.
The utility model can also be:
a biochip reaction box assembly with a waste liquid bag is an integrated structure formed by connecting two or more biochip reaction boxes with waste liquid bags.
The utility model can also be:
a hybridization instrument comprising any one of the above-mentioned biochip reaction cartridge with a waste liquid pocket and/or the above-mentioned biochip reaction cartridge assembly with a waste liquid pocket.
Compared with the prior art, the utility model has the following beneficial effects:
according to the biochip reaction box with the waste liquid bag, the assembly and the hybridization instrument, residual waste liquid of hybridization reaction can be conveniently collected by the added waste liquid bag, the waste liquid is prevented from being thrown into a reaction bin of the instrument, and cleanness of the detection instrument and surrounding environment/air are guaranteed not to be influenced.
Drawings
For a clearer explanation of the embodiments or technical solutions in the prior art of the present application, the drawings used in the description of the embodiments or prior art will be briefly described below, it is obvious that the drawings in the following description are only references to some embodiments in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic front view of a cartridge according to one embodiment of the present invention.
Figure 2 is a schematic perspective view of a cartridge according to one embodiment of the utility model.
Fig. 3 is a schematic view of the back of the cartridge according to one embodiment of the present invention.
Figure 4 is a schematic perspective view of a cartridge according to another embodiment of the present invention.
FIG. 5 is a schematic diagram of a front side of a cassette cover according to one embodiment of the utility model.
Fig. 6 is a perspective view of a cartridge cover according to an embodiment of the present invention.
FIG. 7 is a schematic view of a back side structure of a cassette cover according to one embodiment of the present invention.
FIG. 8 is a schematic perspective view of a cassette cover according to another embodiment of the present invention.
FIG. 9 is a schematic view showing the construction of a biochip reaction cassette with a waste liquid pocket according to one embodiment of the present invention.
1-card box, 2-reaction bin, 3-diversion trench, 4-waste liquid bag, 5-card box cover, 6-image acquisition window, 7-sample addition port, 8-exhaust hole, 9-pressing sheet, 10-first buckle, 11-second buckle, 12-reaction area, 13-third buckle, 14-magnet installation cavity, 15-, 16-, and
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Referring to FIGS. 1 to 9, a biochip reaction cassette with a waste liquid pocket includes a cassette 1, a cassette cover 5; a reaction bin 2, a waste liquid bag 4 and a diversion trench 3 which is connected with the reaction bin 2 and is used as a waste liquid throwing channel after reaction in the reaction bin 2 are arranged in the card box 1; the card box cover 5 is arranged on the card box 1 and seals the reaction bin 2 and the waste liquid bag 4; the waste liquid bag 4 is connected with the diversion trench 3 in the card box 1, the diversion trench 3 can be sealed by the card box cover 5, and the waste liquid bag 4 is used for collecting waste liquid thrown out from the diversion trench 3. In the prior art, the biochip reaction box is generally placed in a hybridization instrument for use, in the using process, the biochip reaction box is in a rotating state, the waste liquid in the reaction bin 2 can be thrown out of the reaction box through the diversion trench 3, and the thrown waste liquid may affect the instrument or the surrounding environment, and therefore, a waste liquid bag 4 is further arranged at the rear end/end part of the card box 1, and the waste liquid bag 4 can collect the waste liquid thrown out of the diversion trench 3, so that the problem of pollution to the instrument and the surrounding environment/air is avoided.
In the above embodiment, besides the way that the diversion trench 3 can be closed by the cassette cover 5, the diversion trench 3 disposed between the reaction chamber 2 and the waste liquid bag 4 may not be sealed by the cassette cover 5, that is, the upper part of the diversion trench 3 is closed by a part of the cassette 1 (not shown in the figure), which may also achieve similar or equivalent functions, and belongs to the protection scope of the present application.
Generally, the cartridge cover 5 is provided with a sample addition port 7 communicating with the reaction chamber 2. The instrument adds the sample into the reaction bin 2 through the sample adding needle step by step through the sample adding opening 7, after the reaction is completed in the reaction bin 2, the turntable is centrifuged, and the waste liquid enters the waste liquid bag 4 through the diversion trench 3 and is collected.
A preferable scheme is that the diversion trench 3 adopts a conical diversion trench, the larger end of the diversion trench is connected with the reaction bin 2, and the smaller end of the diversion trench is connected with the waste liquid bag 4. Namely, the diameter of the conical diversion trench is gradually reduced and the position is gradually increased from the reaction bin 2 to the waste liquid bag 4, so that the opening in the waste liquid bag 4 is ensured to approach the highest position, and the function of the diversion trench is to prevent liquid from flowing back to the reaction bin 2 through the diversion trench 3 in the normal reaction process.
The bottom of the cartridge 1 corresponding to the reaction chamber 2 is a heating zone 12, and the reaction zone 12 can be designed to be thin compared to the prior art in order to increase the temperature increase and decrease speed.
An inwards concave image acquisition window 6 is arranged on the card box cover 5 above the reaction bin 2, so that image acquisition is facilitated.
In addition, the card box cover 5 can be provided with an exhaust hole 8 communicated with the waste liquid bag 4, and in the process of collecting waste liquid, air in the waste liquid bag 4 is exhausted through the exhaust hole 8, so that waste liquid can enter the waste liquid bag 4.
The biochip reaction cassette according to an embodiment may be configured such that a membrane chip is disposed at the bottom of the reaction chamber 2. In order to fix the membrane chip conveniently, a pressing sheet 9 for clamping the reaction chamber 2 and pressing the membrane chip can be arranged on the cassette cover 5. Generally, the cartridge cover 5 is provided with pressing pieces 9 on both sides thereof to press the film chip from both sides.
As shown in fig. 7 and 8, one end of the cassette cover 5 is provided with a first fastener 10 for being clamped into the cassette 1, and the other end of the cassette cover 5 is provided with a second fastener 11 for being clamped into the waste liquid bag 4. And the middle part of the card box cover 5 can also be provided with a third buckle 13, and the third buckle 13 is clamped with the two sides of the position of the diversion trench 3 of the card box 1.
As shown in FIGS. 3 and 4, in order to ensure the cartridge 1 to be completely attached to the reaction plate of the hybridization apparatus, a magnet installation cavity 14 for fixing a magnet may be provided at one end of the cartridge 1, and the magnet is generally matched with the magnet installation cavity 14 in size, so that the cartridge 1 may be stably maintained during the rotation process by the installed magnet.
A biochip reaction cassette assembly with a waste liquid bag is an integrated structure formed by connecting two or more biochip reaction cassettes with waste liquid bags according to the above embodiments, and generally, 4 reaction cassettes are preferably combined together, but not limited thereto.
A hybridization apparatus may include any of the above-described biochip reaction cartridge with a waste sac and/or biochip reaction cartridge assembly with a waste sac.
In summary, a waste liquid collecting device (waste liquid bag 4) is added at the rear part of the waste liquid throwing channel to collect the residual waste liquid of the hybridization reaction, especially the residual PCR product fragments, so as to avoid throwing the residual waste liquid into a reaction bin of an instrument without destination and ensure that the residual product completely enters the air; in addition, the hybridization waste liquid is collected, so that the interior of the instrument is kept dry and clean.
The embodiments are described in a progressive manner in the specification, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
Reference throughout this specification to "one embodiment," "another embodiment," "an embodiment," etc., means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally in this application. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the utility model to effect such feature, structure, or characteristic in connection with other embodiments.
Although the utility model has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure and claims of this application. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (10)
1. A biochip reaction cassette with a waste liquid pocket, comprising:
the device comprises a card box (1), wherein a reaction bin (2), a waste liquid bag (4) and a flow guide groove (3) which is connected with the reaction bin (2) and the waste liquid bag (4) and is used as a waste liquid channel thrown out of the reaction bin (2) are arranged in the card box (1), and the waste liquid bag (4) is used for collecting waste liquid thrown out of the flow guide groove (3);
the card box cover (5), the card box cover (5) set up in on the card box (1), it will reaction storehouse (2), waste liquid bag (4) seal.
2. The biochip reaction cassette with waste liquid pocket according to claim 1, wherein the guide groove (3) is a tapered guide groove, and its larger end is connected to the reaction chamber (2).
3. The biochip reaction cassette with waste liquid pocket according to claim 1, wherein an inwardly concave image capturing window (6) is provided on the cassette cover (5) above the reaction chamber (2).
4. The biochip reaction cassette with waste liquid pocket according to claim 1, wherein the cassette cover (5) is provided with a sample addition port (7) communicating with the reaction cartridge (2).
5. The biochip reaction cassette with waste liquid pocket according to claim 1, wherein the cassette cover (5) is provided with an exhaust hole (8) communicating with the waste liquid pocket (4).
6. The biochip reaction cassette with waste liquid pocket according to claim 1, wherein a membrane chip is provided at the bottom in the reaction chamber (2).
7. The biochip reaction cassette with waste liquid pocket according to claim 6, wherein a pressing piece (9) for clipping the reaction chamber (2) and pressing the membrane chip is provided on the cassette cover (5).
8. The biochip reaction cassette with waste liquid pocket according to claim 1, wherein the cassette cover (5) has a first snap (10) at one end for snapping into the cassette (1), and a second snap (11) at the other end of the cassette cover (5) for snapping into the waste liquid pocket (4).
9. A biochip reaction cartridge assembly with a waste liquid pocket, characterized in that it is an integrated structure formed by connecting two or more biochip reaction cartridges with a waste liquid pocket according to any one of claims 1 to 8.
10. A hybridization instrument comprising the biochip reaction cartridge assembly with a waste liquid pocket according to claim 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122353647.0U CN216639439U (en) | 2021-09-26 | 2021-09-26 | Biochip reaction box with waste liquid bag, assembly and hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122353647.0U CN216639439U (en) | 2021-09-26 | 2021-09-26 | Biochip reaction box with waste liquid bag, assembly and hybridization instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216639439U true CN216639439U (en) | 2022-05-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202122353647.0U Active CN216639439U (en) | 2021-09-26 | 2021-09-26 | Biochip reaction box with waste liquid bag, assembly and hybridization instrument |
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| CN (1) | CN216639439U (en) |
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- 2021-09-26 CN CN202122353647.0U patent/CN216639439U/en active Active
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