CN216622069U - Multi-wavelength scattering polarization fluorescence measuring device - Google Patents
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- CN216622069U CN216622069U CN202122557396.8U CN202122557396U CN216622069U CN 216622069 U CN216622069 U CN 216622069U CN 202122557396 U CN202122557396 U CN 202122557396U CN 216622069 U CN216622069 U CN 216622069U
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Abstract
The utility model provides a multi-wavelength scattering polarization fluorescence measuring device, which comprises a light source, a polarization modulation system, an incident light path, a receiving light path, a first light splitting system, a second light splitting system and a data processing end, wherein, the multi-wavelength light emitted by the light source generates first polarized light after passing through the polarization modulation system, the first polarized light irradiates suspended particles in the water sample through the incident light path, the receiving light path receives scattered light signals from the irradiated suspended particles and transmits the scattered light signals to the first light splitting system, the scattered light signals are divided into a plurality of beams of different parallel scattered light by the first light splitting system, one of the plurality of parallel scattered lights is divided into a plurality of second polarized lights with different polarization states by a second light splitting system, the plurality of second polarized lights are respectively transmitted to the data processing end through the photoelectric converter, and the rest of the plurality of parallel scattered lights are respectively transmitted to the data processing end through the fluorescence detector. The utility model can improve the accuracy of identifying the suspended particles.
Description
Technical Field
The utility model belongs to the technical field of optical measurement, and particularly relates to a multi-wavelength scattering polarization fluorescence measurement device.
Background
The method can quickly, accurately and massively obtain the information of the suspended particles in the water body, and is an important means for ecological research and environmental monitoring. The polarized light is sensitive to the microstructure of a sample, can quickly and effectively obtain the information of suspended particles in a water sample, is an important tool for researching water body characteristics, and has great significance for ecological research, environmental assessment and aquaculture. Optical methods are widely used for detecting particles due to the advantages of high resolution, non-contact, rich information and the like, and currently, methods such as scattering, fluorescence and the like are mainly used. However, the traditional optical method cannot well acquire information of suspended particles, the fluorescence method acquires pigment information of the whole suspended particles in the water body, and the scattering method acquires information such as particle size distribution and concentration of the whole suspended particles in the water body through light intensity angle distribution, so that the identification and classification capability of the suspended particles is limited.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a multi-wavelength scattering polarization fluorescence measuring device, which improves the accuracy of suspended particulate matter identification.
The utility model is realized by the following technical scheme:
a multi-wavelength scattering polarization fluorescence measuring device comprises a light source, a polarization modulation system, an incident light path, a receiving light path, a first light splitting system, a second light splitting system and a data processing end, wherein, the multi-wavelength light emitted by the light source generates first polarized light after passing through the polarization modulation system, the first polarized light irradiates suspended particles in the water sample through the incident light path, the receiving light path receives scattered light signals from the irradiated suspended particles and transmits the scattered light signals to the first light splitting system, the scattered light signals are divided into a plurality of beams of different parallel scattered light by the first light splitting system, one of the parallel scattered lights is divided into a plurality of second polarized lights with different polarization states by a second light splitting system, the second polarized lights are respectively transmitted to the data processing end through the photoelectric converter, and the rest parallel scattered lights in the parallel scattered lights are respectively transmitted to the data processing end through the fluorescence detector.
Furthermore, the polarization modulation system comprises a plurality of first gears which are rotatably arranged and a plurality of driving assemblies which are respectively connected with the first gears and used for driving the first gears to rotate, the first gears are arranged at intervals, the centers of the first gears are all located on the same straight line, a through hole is formed in the center of each first gear, and a wave plate is arranged in each through hole.
Further, the number of the first gears is more than two.
Further, the incident light path comprises a convex lens for converging the first polarized light at the suspended particles.
Further, the receiving optical path comprises a collimating system for modulating the scattered light signal into a single beam of parallel light.
Further, the first light splitting system comprises a first light splitting prism, a second light splitting prism, a first light filter, a second light filter and a third light filter, the first light splitting prism is arranged on one side, far away from the suspended particulate matter, of the receiving light path, the second light splitting prism is arranged on the transmission light path of the first light splitting prism, the first light filter is arranged on the reflection light path of the first light splitting prism, the second light filter is arranged on the reflection light path of the second light splitting prism, the third light filter is arranged on the transmission light path of the second light splitting prism, the number of the fluorescent detectors is two, and the two fluorescent detectors are respectively arranged on the emergent light paths of the first light filter and the second light filter.
Further, the light source includes first laser instrument, a plurality of second laser instrument and a plurality of dichroic mirror, and a plurality of dichroic mirror sets gradually on the transmission light path of first laser instrument, and a plurality of second laser instruments are the same and the one-to-one with a plurality of dichroic mirror's quantity, and the second laser instrument sets up on the reflection light path of corresponding dichroic mirror.
Further, the photoelectric converter is a photomultiplier tube.
Compared with the prior art, the utility model has the beneficial effects that: synchronously measuring the polarization scattering signal and the fluorescence signal of the suspended particulate matters in the water sample through multi-wavelength light incidence, and achieving the purpose of distinguishing and identifying target suspended particulate matters by combining the polarization scattering characteristic and the fluorescence excitation characteristic of the scattered light of the suspended particulate matters; receive the polarization signal and the fluorescence signal of suspended particles scattered light simultaneously, make full use of the fluorescence characteristic of suspended particles, increased the information dimension, the characteristic data of suspended particles has been richened, utilize the polarization characteristic to know the structure of suspended particles, know the constitution condition of suspended particles pigment through arousing the fluorescence characteristic, with the suspended particles who realizes discernment and distinguish in the water sample, owing to combined the structure and pigment scheduling factor of suspended particles, can improve the accuracy of particle discernment greatly.
Drawings
FIG. 1 is a schematic structural diagram of a multi-wavelength scattering polarization fluorescence measuring device according to the present invention;
FIG. 2 is a schematic structural diagram of a polarization modulation system in the multi-wavelength scattering polarization fluorescence measuring apparatus according to the present invention;
FIG. 3 is a schematic structural diagram of a light source in the multi-wavelength scattering polarization fluorescence measuring apparatus according to the present invention.
In the figure, 1-light source, 11-first laser, 12-second laser, 13-dichroic mirror, 2-polarization modulation system, 21-first gear, 22-driving component, 23-wave plate, 3-incident light path, 4-receiving light path, 5-first light splitting system, 51-first light splitting prism, 52-second light splitting prism, 53-first light filter, 54-second light filter, 55-third light filter, 6-second light splitting system, 7-data processing terminal, 8-photoelectric converter, 9-fluorescence detector, 10-suspended particulate matter.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures. Meanwhile, in the description of the present invention, the terms "first", "second", and the like are used only for distinguishing the description, and are not to be construed as indicating or implying relative importance.
It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", and the like refer to the orientation or positional relationship shown in the drawings, or the orientation or positional relationship which the utility model product is conventionally placed in use, and are only for convenience of describing the present invention and simplifying the description, but do not refer to or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.
Referring to fig. 1, fig. 1 is a schematic structural diagram of a multi-wavelength scattering polarization fluorescence measuring apparatus according to the present invention. The utility model provides a multi-wavelength scattering polarization fluorescence measuring device, including light source 1, polarization modulation system 2, incident light path 3, receiving optical path 4, first light splitting system 5, second light splitting system 6, photoelectric converter 8, fluorescence detector 9 and data processing end 7, wherein, the multi-wavelength light that light source 1 sent loops through polarization modulation system 2, incident light path 3 projects on the suspended particles 10 in the water sample, the scattered light signal of the suspended particles 10 that are shone loops through first light splitting system 5, fluorescence detector 9 transmits data processing end 7, and the scattered light signal of the suspended particles 10 that are shone passes through first light splitting system 5 in proper order, second light splitting system 6, photoelectric conversion it transmits data processing end 7. The light source 1 emits multi-wavelength light and the multi-wavelength light is incident on the suspended particles 10 in the water sample, the polarization scattering signals and the multiple excitation fluorescent signals of the suspended particles 10 in the water sample are synchronously measured, and the suspended particles 10 in the water sample are identified and distinguished through the polarization characteristics and the multiple fluorescence characteristics of the suspended particles 10 in the water sample contained in the scattered light.
Specifically, the polarization modulation system 2 and the incident light path 3 are disposed on an emission light path of the light source 1 and located between the light source 1 and the suspended particulate matter 10 in the water sample, the receiving light path 4 is disposed at a backscattering angle of the suspended particulate matter 10 relative to the incident light path 3, the first light splitting system 5 is disposed on an exit light path of the receiving light path 4, the first light splitting system 5 has a plurality of exit light paths, wherein a second light splitting system 6 is disposed on one exit light path of the plurality of exit light paths of the first light splitting system 5, a fluorescence detector 9 is disposed on the remaining exit light path of the plurality of exit light paths of the first light splitting system 5, the second light splitting system 6 has a plurality of exit light paths, photoelectric converters 8 are disposed on the plurality of exit light paths of the second light splitting system 6, and both the fluorescence detector 9 and the photoelectric converters 8 are connected to the data processing terminal 7. The multi-wavelength light emitted by the light source 1 passes through the polarization modulation system 2 to generate first polarized light in a specific polarization state, the first polarized light passes through the incident light path 3 and irradiates suspended particulate matters 10 in a water sample, the receiving light path 4 receives scattered light signals from the irradiated suspended particulate matters 10 and transmits the scattered light signals to the first light splitting system 5, the scattered light signals are divided into a plurality of different parallel scattered lights by the first light splitting system 5, one of the plurality of parallel scattered lights is divided into a plurality of second polarized lights in different polarization states by the second light splitting system 6, the plurality of second polarized lights are respectively transmitted to the data processing end 7 through the photoelectric converter 8, and the rest parallel scattered lights in the plurality of parallel scattered lights are respectively transmitted to the data processing end 7 through the fluorescent detector 9.
Referring to fig. 3, fig. 3 is a schematic structural diagram of a light source in the multi-wavelength scattering polarization fluorescence measuring apparatus according to the present invention. In an embodiment, the light source 1 includes a first laser 11, a plurality of second lasers 12, and a plurality of dichroic mirrors 13, the plurality of dichroic mirrors 13 are sequentially disposed on a transmission light path of the first laser 11, the plurality of second lasers 12 and the plurality of dichroic mirrors 13 are equal in number and correspond to each other one by one, and the second lasers 12 are disposed on reflection light paths of the corresponding dichroic mirrors 13. The wavelength of first laser 11 and a plurality of second laser 12 is all different, and the wavelength of first laser 11 and a plurality of second laser 12 can be decided according to the kind of the suspended particles 10 of the water sample of needs measurement, through setting up first laser 11 on dichroic mirror 13's transmission light path, sets up second laser 12 on corresponding dichroic mirror 13's reflection light path to the light that first laser 11 and a plurality of second laser 12 emitted overlaps into a light after passing through dichroic mirror 13 transmission and reflection respectively, forms the multi-wavelength light. Thereby realizing that the light source 1 emits multi-wavelength light. The number of the second lasers 12 is determined according to the wavelength number of the multi-wavelength light required, if the suspended particle 10 is algae and two second lasers 12 are used, the first laser 11 may be a 440nm laser, and the two second lasers 12 are respectively a 530nm laser and a 650nm laser.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a polarization modulation system in the multi-wavelength scattering polarization fluorescence measurement apparatus according to the present invention. In an embodiment, the polarization modulation system 2 includes a plurality of first gears 21 rotatably disposed and a plurality of driving assemblies 22 respectively connected to the plurality of first gears 21 for driving the first gears 21 to rotate, the plurality of first gears 21 are disposed at intervals, centers of the plurality of first gears 21 are all located on the same straight line, a through hole is formed in the center of the first gear 21, and a wave plate 23 is disposed in the through hole. The driving assembly 22 drives the corresponding first gear 21 to rotate so as to control the angle of the wave plate 23 at the center of the first gear 21, so as to change the polarization state of the incident light by means of the wave plate 23, so that when the multi-wavelength light emitted by the light source 1 passes through a plurality of wave plates 23, the first polarized light with any polarization state can be modulated. The wave plate 23 may adopt an 1/2 wave plate. In one embodiment, the number of the first gears 21 is two or more. The multi-wavelength light emitted by the light source 1 passes through more than two wave plates 23 to produce the first polarized light in any polarization state. In one embodiment, the driving assembly 22 includes a motor and a second gear disposed on the driving end of the motor, the second gear being in meshing transmission with the first gear 21. The motor drives the first gear 21 to rotate through the second gear, so as to control the angle of the wave plate 23 at the center of the first gear 21.
In an embodiment, the incident light path 3 comprises a convex lens for converging the first polarized light at the suspended particles 10. The first polarized light is converged into a focus through the convex lens, and the suspended particles 10 in the water sample generate scattered light signals through the focus.
In an embodiment, the receiving optical path 4 comprises a collimating system for modulating the scattered light signal into a single beam of parallel light. The collimation system receives scattered light signals from the irradiated suspended particles 10, modulates the received scattered light signals into a single beam of parallel light, and transmits the single beam of parallel light to the first light splitting system 5.
In one embodiment, the first light splitting system 5 includes a first light splitting prism 51, a second light splitting prism 52, a first optical filter 53, a second optical filter 54 and a third optical filter 55, the first light splitting prism 51 is disposed on the side of the receiving light path 4 far away from the suspended particulate matter 10, the second light splitting prism 52 is disposed on the transmitting light path of the first light splitting prism 51, the first optical filter 53 is disposed on the reflecting light path of the first light splitting prism 51, the second optical filter 54 is disposed on the reflecting light path of the second light splitting prism 52, the third optical filter 55 is disposed on the transmitting light path of the second light splitting prism 52, the number of the fluorescence detectors 9 is two, and the two fluorescence detectors 9 are disposed on the outgoing light paths of the first optical filter 53 and the second optical filter 54 respectively. A first beam splitter prism 51 is arranged on the outgoing light path of the receiving light path 4, the receiving light path 4 modulates the received scattered light signal into a bundle of parallel light, the parallel light enters the first beam splitter prism 51, is reflected by the first beam splitter prism 51 to form first parallel scattered light, the first parallel scattered light enters the fluorescence detector 9 after being filtered by the first optical filter 53, and meanwhile, the parallel light forms second parallel scattered light after being transmitted by the first beam splitter prism 51, the second parallel scattered light enters the second beam splitter prism 52, is reflected by the second beam splitter prism 52 to form third parallel scattered light, the third parallel scattered light enters the fluorescence detector 9 after being filtered by the second optical filter 54, meanwhile, the second parallel scattered light forms fourth parallel scattered light after being transmitted by the second beam splitter prism 52, and the fourth parallel scattered light enters the second light splitting system 6 after passing through the third optical filter 55. Therefore, the first light splitting system 5 splits the received parallel light into three different parallel scattered lights, one parallel scattered light enters the second light splitting system 6, and the other two parallel scattered lights enter the data processing terminal 7 through the two fluorescence detectors 9 respectively. Preferably, the first filter 53, the second filter 54 and the second filter 54 are filters with different wavelengths, for example, 685nm bandpass filters can be used as the first filter 53, 600nm bandpass filters can be used as the second filter 54, and 440nm bandpass filters can be used as the third filter 55.
In one embodiment, the second light splitting system 6 includes a third light splitting prism, a fourth light splitting prism, a fifth light splitting prism, a first polarizer, a second polarizer, a third polarizer and a fourth polarizer, the third light splitting prism is disposed on a side of the first light splitting system away from the receiving optical path, the fourth light splitting prism is disposed on a transmission optical path of the third light splitting prism, the first polarizer is disposed on a reflection optical path of the third light splitting prism, the second polarizer is disposed on a reflection optical path of the fourth light splitting prism, the fifth light splitting prism is disposed on a transmission optical path of the fourth light splitting prism, the third polarizer is disposed on a reflection optical path of the fifth light splitting prism, the fourth polarizer is disposed on a transmission optical path of the fifth light splitting prism, the number of the photoelectric converters 8 is four, the four photoelectric converters 8 are disposed on the first polarizer, the second polarizer, the third polarizer, the fifth light splitting prism, the fourth polarizer 8, And the emergent light path of the fourth polaroid. The second light splitting system 6 adopts an amplitude splitting method, parallel scattered light filtered by a third optical filter enters a third light splitting prism, the parallel scattered light is split into four emergent lights after being transmitted and reflected by the third light splitting prism, the fourth light splitting prism and the fifth light splitting prism, the four emergent lights are respectively subjected to polarization detection by a first polarizing film, a second polarizing film, a third polarizing film and a fourth polarizing film to form four beams of second polarized lights with different polarization states, and the four beams of second polarized lights are respectively converted by four photoelectric converters 8 and then transmitted to a data processing end. The first polaroid, the second polaroid, the third polaroid and the fourth polaroid are analyzer plates with different angles. The types of the polarization analyzing sheets can be selected according to actual conditions, for example, a horizontal polarization sheet, a 135-degree polarization sheet, a 45-degree polarization sheet and a left-handed polarization sheet are respectively added to the light paths of the four emergent lights, and the left-handed polarization sheet, i.e., the 90-degree polarization sheet, is added with an 1/4 wave plate, so that the polarization analyzing of the emergent lights is realized, and the four beams of second polarized lights with different polarization states are obtained. In one embodiment, the photoelectric converter 8 is a photomultiplier tube. The second light splitting system 6 splits the second polarized light into a plurality of beams with different polarization states, and the beams are converted and amplified by the photomultiplier tube and then transmitted to the data processing end 7.
The following describes the implementation process of the multi-wavelength scattering polarization fluorescence measurement device of the present invention:
the light emitted by the first laser 11 and the plurality of second lasers 12 is respectively transmitted and reflected by the dichroic mirror 13 and then coincided into multi-wavelength light, the multi-wavelength light is incident to the polarization modulation system 2, the angle of the wave plate 23 is adjusted by rotating the first gear 21, so that the multi-wavelength light is modulated into first polarized light in a specific polarization state after passing through the plurality of wave plates 23, the first polarized light is converged into a focus by the convex lens, the suspended particulate matter 10 in the water sample passes through the focus to generate a scattered light signal, the collimation system receives the scattered light signal from the irradiated suspended particulate matter 10 and modulates the received scattered light signal into single-beam parallel light, the parallel light is incident to the first light splitting system 5 and is divided into three beams of parallel scattered light by the first light splitting prism 51 and the second light splitting prism 52, wherein the two beams of parallel scattered light are respectively filtered by the first optical filter 53 and the second optical filter 54 in specific wavelength to form parallel scattered light in different wavelengths, the fluorescence signals are respectively emitted to a fluorescence detector 9, and the fluorescence detector 9 collects fluorescence signals and transmits the collected fluorescence signals to a data processing terminal 7; the remaining parallel scattered light forms parallel scattered light with a specific wavelength through a third optical filter 55 with a specific wavelength and is incident on the second light splitting system 6, the second light splitting system 6 can adopt an amplitude splitting method, that is, the received parallel scattered light is split into four emergent lights, analyzer plates with different angles are respectively arranged on the light path of each emergent light, so that the emergent lights are analyzed and polarized, four beams of second polarized light with different polarization states are formed after the four emergent lights are analyzed and polarized, and the four beams of second polarized light are respectively converted, amplified and transmitted through four photomultiplier tubes and then transmitted to the data processing terminal 7. Thereby data processing end 7 obtains the polarization characteristic and a plurality of fluorescence characteristic of the suspended particles 10 in the water sample simultaneously, and data processing end 7 calculates the fluorescence excitation light intensity according to presetting procedure and a plurality of fluorescence characteristic to the realization is unscrambled the fluorescence characteristic of suspended particles 10, and data processing end 7 calculates the Stokes vector according to presetting procedure and polarization characteristic simultaneously, in order to realize the polarization characteristic unscrambling of suspended particles 10. Under a certain incident polarization state, due to different pigment compositions in the suspended particulate matter 10, the wavelength and the intensity of the excited fluorescence have specific performances due to different suspended particulate matter 10, meanwhile, certain combinations of Stokes vectors can well represent the characteristics of the suspended particulate matter 10, and the polarization property of the specificity is a specific parameter. The polarization parameters of the suspended particulate matter 10 can be extracted through the type of incident light, the selection of the polarization components, the operation and combination between the polarization components, and the like, so that the identification of the suspended particulate matter 10 is realized. Therefore, the data processing end 7 combines the factors such as the structure and the pigment of the suspended particulate matter 10 by using the data of the excitation fluorescence intensity and the Stokes vector, and the accuracy of identifying and distinguishing the suspended particulate matter 10 is greatly improved.
Compared with the prior art, the utility model has the beneficial effects that: synchronously measuring the polarization scattering signal and the fluorescence signal of the suspended particulate matter 10 in the water sample through multi-wavelength light incidence, and achieving the purpose of distinguishing and identifying the target suspended particulate matter 10 by combining the polarization scattering characteristic and the fluorescence excitation characteristic of the scattered light of the suspended particulate matter 10; receive the polarization signal and the fluorescence signal of suspended particles 10 scattered light simultaneously, make full use of the fluorescence characteristic of suspended particles 10, the information dimension has been increased, the characteristic data of suspended particles 10 has been richened, utilize the polarization characteristic to know the structure of suspended particles 10, know the constitution condition of suspended particles 10 pigment through arousing the fluorescence characteristic, with the suspended particles 10 in realizing discernment and distinguishing the water sample, owing to factors such as the structure and the pigment that have combined suspended particles 10, can improve the accuracy of particle discernment greatly.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention will still fall within the scope of the technical solution of the present invention without departing from the content of the technical solution of the present invention.
Claims (8)
1. The multi-wavelength scattering polarization fluorescence measurement device is characterized by comprising a light source, a polarization modulation system, an incident light path, a receiving light path, a first light splitting system, a second light splitting system and a data processing end, wherein multi-wavelength light emitted by the light source passes through the polarization modulation system to generate first polarized light, the first polarized light irradiates suspended particulate matters in a water sample through the incident light path, the receiving light path receives scattered light signals from the irradiated suspended particulate matters and transmits the scattered light signals to the first light splitting system, the scattered light signals are split into a plurality of different parallel scattered light by the first light splitting system, one of the plurality of parallel scattered light is split into a plurality of second polarized light with different polarization states by the second light splitting system, the plurality of second polarized light are respectively transmitted to the data processing end through a photoelectric converter, and the rest of the plurality of parallel scattered light are respectively transmitted to the data processing end through a fluorescence detector .
2. The multi-wavelength scattering polarization fluorescence measuring device according to claim 1, wherein the polarization modulation system comprises a plurality of first gears and a plurality of driving components, the first gears are rotatably disposed, the driving components are respectively connected with the first gears and are used for driving the first gears to rotate, the first gears are disposed at intervals, centers of the first gears are all located on the same straight line, a through hole is formed in the center of each first gear, and a wave plate is disposed in the through hole.
3. The multi-wavelength scatter polarization fluorescence measuring device of claim 2, wherein the number of the first gears is two or more.
4. The multi-wavelength scatter polarization fluorescence measurement device of claim 1, wherein the light source comprises a first laser, a plurality of second lasers and a plurality of dichroic mirrors, the plurality of dichroic mirrors are sequentially disposed on an emission light path of the first laser, the plurality of second lasers are in the same number as the plurality of dichroic mirrors and in one-to-one correspondence, and the second lasers are disposed on reflection light paths of the corresponding dichroic mirrors.
5. The apparatus according to claim 1, wherein the first beam splitter system includes a first beam splitter prism, a second beam splitter prism, a first optical filter, a second optical filter, and a third optical filter, the first beam splitter prism is disposed on a side of the receiving optical path away from the suspended particulate matter, the second beam splitter prism is disposed on the transmitting optical path of the first beam splitter prism, the first optical filter is disposed on the reflecting optical path of the first beam splitter prism, the second optical filter is disposed on the reflecting optical path of the second beam splitter prism, the third optical filter is disposed on the transmitting optical path of the second beam splitter prism, the number of the fluorescence detectors is two, and two of the fluorescence detectors are disposed on the emitting optical paths of the first optical filter and the second optical filter, respectively.
6. The multi-wavelength scatter polarization fluorescence measurement device of claim 1, wherein the incident light path comprises a convex lens for concentrating the first polarized light at the suspended particulate matter.
7. The multi-wavelength scatter polarization fluorescence measurement device of claim 1, wherein the receive optical path comprises a collimation system for modulating the scattered light signal into a single beam of parallel light.
8. The multi-wavelength scatter polarization fluorescence measurement device of claim 1, wherein the photoelectric converter is a photomultiplier tube.
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Effective date of registration: 20230113 Address after: 528225 Room 306, Floor 3, Building A, Group 1, Phase I, Guangdong Biomedical Industry Base, Xianxi Section, National Highway 321, Shishan Town, Nanhai District, Foshan City, Guangdong Province Patentee after: Foshan Linguistic Technology Co.,Ltd. Address before: 528225 Foshan Institute of science and technology, Xianxi reservoir West Road, Shishan town, Nanhai District, Foshan City, Guangdong Province Patentee before: FOSHAN University Patentee before: Foshan Linguistic Technology Co.,Ltd. |