CN216585032U

CN216585032U - Carbon dioxide incubator

Info

Publication number: CN216585032U
Application number: CN202123215058.2U
Authority: CN
Inventors: 宋娟; 王文龙; 常艺海; 秦留勇; 常小利; 莫李波
Original assignee: Zhengzhou Saibo Biotechnology Co ltd
Current assignee: Zhengzhou Saibo Biotechnology Co ltd
Priority date: 2021-12-20
Filing date: 2021-12-20
Publication date: 2022-05-24
Anticipated expiration: 2031-12-20

Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to a carbon dioxide incubator which comprises a box body, wherein a box door is arranged at the front end of the box body, a culture cavity, a draining cavity and an equipment cavity are arranged in the box body, a storage rack is arranged in the culture cavity, a heater and a humidifier are arranged in the side wall of the culture cavity, an air cylinder is arranged outside the draining cavity, an air cylinder seat of the air cylinder extends into the draining cavity, a draining mechanism is arranged in the draining cavity and comprises a bidirectional synchronous telescopic electric push rod, a draining rod and a water collecting tank, the two free ends of the bidirectional synchronous telescopic electric push rod are connected with vertical rods, the bottom ends of the vertical rods are connected with draining parts, the draining rod is fixedly connected with a piston rod of the air cylinder, a water absorbing sponge is sleeved outside the draining rod, the water collecting tank is positioned below the draining rod, and a temperature and humidity sensor and a carbon dioxide generator are arranged in the equipment cavity. The utility model can provide a good environment for cell culture and can avoid the phenomenon that water drops on the top wall of the incubator fall into the culture dish to influence the growth of cells.

Description

Carbon dioxide incubator

Technical Field

The utility model belongs to the technical field of cell culture, and particularly relates to a carbon dioxide incubator.

Background

The carbon dioxide incubator simulates the growth environment of similar cells or tissues in organisms in the incubator body, and requires stable temperature (37 ℃) and stable CO₂The device for in vitro culture of cells or tissues by using the level (5%), constant pH value (pH value: 7.2-7.4) and higher relative saturation humidity (95%) is an advanced instrument for cell, tissue and bacteria culture and is a key device necessary for developing immunology, oncology, genetics and bioengineering. The existing carbon dioxide incubator has simple structure and single function; in use, water droplets often appear on the top wall of the incubator and if they fall into the cell culture dish they can interfere with the growth of the cells.

Therefore, improvement is required.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems in the prior art, the utility model provides a carbon dioxide incubator which comprises a box body, wherein a box door is arranged at the front end of the box body, a culture chamber, a draining chamber and an equipment chamber are arranged in the box body, the culture chamber is positioned on the left side of the box body, the draining chamber and the equipment chamber are both positioned on the right side of the box body, the culture chamber is separated from the draining chamber and the equipment chamber through vertical partition plates, a plurality of layers of object placing net racks are fixedly arranged in the culture chamber, a heater is arranged in the left side wall of the culture chamber, a humidifier is arranged in the top wall of the culture chamber, and an air outlet of the humidifier extends into the culture chamber;

the device comprises a device cavity, a draining cavity, a laser range finder, a draining mechanism, a draining piece and a water collecting tank, wherein the draining cavity is positioned above the device cavity and is separated from the device cavity through a horizontal partition plate, the outer part of the draining cavity is provided with an air cylinder, an air cylinder seat of the air cylinder extends into the draining cavity and is sealed and fixedly connected with the side wall of the draining cavity, the part of the air cylinder seat extending into the draining cavity is provided with the laser range finder, the draining mechanism is arranged in the draining cavity and comprises a bidirectional synchronous telescopic electric push rod, a draining rod and a water collecting tank, the bidirectional synchronous telescopic electric push rod is fixed at the top of the draining cavity, two free ends of the bidirectional synchronous telescopic electric push rod are fixedly connected with a vertical rod, the bottom end of the vertical rod is fixedly connected with the draining piece, the draining piece is in an inverted U shape and is positioned below the bidirectional synchronous telescopic electric push rod and parallel to the two, and the middle part of the draining rod is fixedly connected with a piston rod of the air cylinder, the water collecting tank is arranged on the horizontal partition plate and is positioned below the draining rod;

be equipped with temperature and humidity sensor and carbon dioxide generator in the equipment chamber, temperature and humidity sensor installs on vertical baffle, and temperature and humidity sensor's measuring probe stretches into cultivates the chamber, carbon dioxide generator sets up the bottom in the equipment chamber, carbon dioxide generator's gas outlet is connected with the trachea, and the trachea passes vertical baffle and stretches into in cultivateing the chamber.

Preferably, the temperature and humidity sensor is a HCSM6703-01 waterproof temperature and humidity sensor and is used for detecting the temperature, the humidity and the carbon dioxide concentration in the culture cavity.

Preferably, the laser range finder adopts a YHJ-200J waterproof laser range finder for detecting the distance between the water-absorbing sponge and the cylinder, and the length of the piston rod of the cylinder for stretching and contracting can be set according to the measured value of the laser range finder, so that the operation of wiping the water-absorbing sponge and draining the water-absorbing sponge by the draining mechanism can be better controlled.

Preferably, the inner cavity of the draining piece is matched with the appearance of the draining rod.

Preferably, the height of the draining rod is consistent with that of the draining piece, and the height of the top of the water-absorbing sponge is consistent with that of the inner wall of the top of the culture cavity.

Preferably, the outer wall of the box door is provided with a control panel, and the temperature and humidity sensor, the laser range finder, the heater, the humidifier and the carbon dioxide generator are all electrically connected with the control panel.

The utility model also includes other components that enable the normal use of a carbon dioxide incubator, all by conventional means in the art. In addition, the devices or components which are not limited in the utility model all adopt the conventional technical means in the field, such as a bidirectional synchronous telescopic electric push rod, an air cylinder, an exhaust fan, a timer, a heater, a humidifier, a carbon dioxide generator and the like.

The working principle of the utility model is that the bidirectional synchronous telescopic electric push rod and the air cylinder in the scheme are both waterproof; when the device is used, the culture dishes containing the tissue cells can be placed on each object placing net rack in a layered mode, the temperature, the humidity and the carbon dioxide concentration in the culture cavity are set, then the box door is closed, after the device is used for a period of time, water drops can be generated on the inner wall of the top of the culture cavity, the piston rod can be repeatedly stretched or contracted through the control cylinder, the water dropping rod is driven to move back and forth, and therefore the water absorbing sponge can wipe off the generated water drops, and the water drops are prevented from dropping into the culture dishes; when excessive water is accumulated on the water absorption sponge, the two free ends can be simultaneously contracted by controlling the bidirectional synchronous telescopic electric push rod, so that the two vertical rods are driven to move oppositely, and the two water draining pieces extrude the water absorption sponge on the water draining rod from two sides so as to drain water in the water absorption sponge; the box body is also provided with a timer and an exhaust fan, the timer can be used for timing the work of the air cylinder, and the exhaust fan is used for exhausting moisture in the box body.

The utility model has the advantages of reasonable structural design and convenient operation, provides a good growth environment for cell culture, can prevent water drops on the top wall of the incubator from falling into a culture dish to influence cell growth, and can drain the water-absorbing sponge in time by the draining mechanism to ensure the moisture absorption effect of the water-absorbing sponge, thereby better maintaining the growth environment of cells.

Drawings

The utility model is further illustrated with reference to the following figures and examples.

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic structural view of the draining mechanism of the present invention.

In the figure: 1. the device comprises a box body, a culture chamber, a water draining chamber, a device chamber, a storage rack, a heater, a humidifier, an air cylinder, a laser range finder, a water draining mechanism, a bidirectional synchronous telescopic electric push rod, a water draining rod, a water collecting tank, a vertical rod, a water draining piece, a water absorbing sponge, a temperature and humidity sensor, a carbon dioxide generator and an air pipe, wherein the culture chamber is 2, the water draining chamber is 3, the equipment chamber is 4, the storage rack is 5, the heater is 6, the humidifier is 7, the air cylinder is 8, the laser range finder is 9, the water draining mechanism is 10, the bidirectional synchronous telescopic electric push rod is 11, the water draining rod is 12, the water collecting tank is 13, the vertical rod is 14, the water draining piece is 15, the water absorbing sponge is 16, the temperature and humidity sensor is 18, and the carbon dioxide generator is 19.

Detailed Description

The present invention will be described more clearly with reference to the accompanying drawings, which are included to illustrate and not to limit the present invention. All other embodiments, which can be obtained by those skilled in the art without any inventive step based on the embodiments of the present invention, should be included in the scope of the present invention.

Examples

As shown in fig. 1-2, the utility model provides a carbon dioxide incubator, which comprises a box body 1, wherein a box door (not shown in the figure) is arranged at the front end of the box body 1, a culture chamber 2, a draining chamber 3 and an equipment chamber 4 are arranged in the box body 1, the culture chamber 2 is positioned at the left side of the box body 1, the draining chamber 3 and the equipment chamber 4 are both positioned at the right side of the box body 1, the culture chamber 2 is separated from the draining chamber 3 and the equipment chamber 4 by vertical partition plates, a plurality of layers of object placing net racks 5 are fixedly arranged in the culture chamber 2, a heater 6 is arranged in the left side wall of the culture chamber 2, a humidifier 7 is arranged in the top wall of the culture chamber 2, and an air outlet of the humidifier 7 extends into the culture chamber 2;

the device comprises a draining cavity 3, a cylinder 8, a cylinder seat of the cylinder 8, a draining cavity 3, a draining rod 12 and a water collecting tank 13, wherein the draining cavity 3 is positioned above the device cavity 4, the draining cavity 3 and the device cavity 4 are separated by a horizontal partition plate, the cylinder 8 is arranged outside the draining cavity 3, the cylinder seat of the cylinder 8 extends into the draining cavity 3 and is sealed and fixedly connected with the side wall of the draining cavity 3, a laser range finder 9 is arranged on the part of the cylinder seat extending into the draining cavity 3, a draining mechanism 10 is arranged in the draining cavity 3, the draining mechanism 10 comprises a bidirectional synchronous telescopic electric push rod 11, a draining rod 12 and a water collecting tank 13, the bidirectional synchronous telescopic electric push rod 11 is fixed at the top of the draining cavity 3, two free ends of the bidirectional synchronous telescopic electric push rod 11 are fixedly connected with a vertical rod 14, a draining part 15 is fixedly connected at the bottom end of the vertical rod 14, the draining part 15 is in an inverted U shape, and the draining rod 12 is positioned below the bidirectional synchronous telescopic electric push rod 11, the two parts are parallel to each other, the middle part of the draining rod 12 is fixedly connected with a piston rod of the air cylinder 8, the outer part of the draining rod 12 is sleeved with a water absorption sponge 16, a notch for the water absorption sponge 16 and the draining rod 12 to pass through is formed in the vertical partition plate, and the water collecting tank 13 is arranged on the horizontal partition plate and is positioned below the draining rod 12;

be equipped with temperature and humidity sensor 17 and carbon dioxide generator 18 in the equipment chamber 4, temperature and humidity sensor 17 installs on vertical baffle, and temperature and humidity sensor 17's measuring probe stretches into cultivates in the chamber 2, carbon dioxide generator 18 sets up the bottom in equipment chamber 4, carbon dioxide generator 18's gas outlet is connected with trachea 19, and trachea 19 passes vertical baffle and stretches into in cultivateing chamber 2.

The temperature and humidity sensor 17 adopts a HCSM6703-01 waterproof temperature and humidity sensor and is used for detecting the temperature, the humidity and the carbon dioxide concentration in the culture cavity 2.

The laser range finder 9 adopts a YHJ-200J waterproof laser range finder for detecting the distance between the water-absorbing sponge 16 and the air cylinder 8, and the length of the stretching and shrinking of the piston rod of the air cylinder 8 can be set according to the measured value of the laser range finder 9, so that the water wiping operation of the water-absorbing sponge 16 and the water draining operation of the water draining mechanism 10 on the water-absorbing sponge 16 can be better controlled.

The inner cavity of the draining piece 15 is matched with the shape of the draining rod 12.

The height of the draining rod 12 is consistent with that of the draining piece 15, and the height of the top of the water absorbing sponge 16 is consistent with that of the inner wall of the top of the culture cavity 2.

A control panel (not shown in the figure) is arranged on the outer wall of the box door (not shown in the figure), and the temperature and humidity sensor 17, the laser range finder 9, the heater 6, the humidifier 7 and the carbon dioxide generator 18 are all electrically connected with the control panel (not shown in the figure).

The working principle of the utility model is that the bidirectional synchronous telescopic electric push rod 11 and the cylinder 8 in the scheme are both waterproof; when the device is used, culture dishes containing tissue cells can be placed on each storage rack 5 in a layered mode, the temperature, the humidity and the carbon dioxide concentration in the culture cavity 2 are set, then a box door (not shown in the figure) is closed, after the device is used for a period of time, water drops can be generated on the inner wall of the top of the culture cavity 2, the piston rod can be repeatedly stretched or contracted through the control cylinder 8, the water draining rod 12 is driven to move back and forth, and then the water absorbing sponge 16 can wipe off the generated water drops to prevent the water drops from dropping into the culture dishes; when excessive water is accumulated on the water absorption sponge 16, the two free ends can be simultaneously contracted by controlling the bidirectional synchronous telescopic electric push rod 11, so that the two vertical rods 14 are driven to move oppositely, and the two water draining pieces 15 squeeze the water absorption sponge 16 on the water draining rod 12 from two sides so as to drain water in the water absorption sponge 16; the cabinet 1 is further provided with a timer (not shown) for timing the operation of the cylinder 8 and an exhaust fan (not shown) for exhausting moisture from the cabinet 1.

Having described embodiments of the present invention, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the illustrated embodiments.

Claims

1. A carbon dioxide incubator which characterized in that: the box comprises a box body, wherein a box door is arranged at the front end of the box body, a culture chamber, a draining chamber and an equipment chamber are arranged in the box body, the culture chamber is positioned on the left side of the box body, the draining chamber and the equipment chamber are both positioned on the right side of the box body, the culture chamber is separated from the draining chamber and the equipment chamber through vertical partition plates, a plurality of layers of object placing net racks are fixedly arranged in the culture chamber, a heater is arranged in the wall of the left side of the culture chamber, a humidifier is arranged in the top wall of the culture chamber, and an air outlet of the humidifier extends into the culture chamber;

2. The carbon dioxide incubator of claim 1, wherein: the temperature and humidity sensor adopts a HCSM6703-01 waterproof temperature and humidity sensor and is used for detecting the temperature, the humidity and the carbon dioxide concentration in the box body.

3. The carbon dioxide incubator of claim 1, wherein: the laser range finder adopts a YHJ-200J waterproof type laser range finder and is used for detecting the distance between the water-absorbing sponge and the air cylinder.

4. The carbon dioxide incubator of claim 1, wherein: the inner cavity of the draining piece is matched with the shape of the draining rod.

5. The carbon dioxide incubator of claim 1, wherein: the height of the draining rod is consistent with that of the draining piece, and the height of the top of the water-absorbing sponge is consistent with that of the inner wall of the top of the culture cavity.

6. The carbon dioxide incubator of claim 1, wherein: the outer wall of the box door is provided with a control panel, and the temperature and humidity sensor, the laser range finder, the heater, the humidifier and the carbon dioxide generator are all electrically connected with the control panel.