CN216560275U

CN216560275U - Intelligent quantitative detection auxiliary equipment for mycotoxin quantum dot test paper strip

Info

Publication number: CN216560275U
Application number: CN202123210947.XU
Authority: CN
Inventors: 张兆威; 胡小风; 陈小媚; 李培武; 汪雪芳
Original assignee: Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
Current assignee: Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
Priority date: 2021-12-20
Filing date: 2021-12-20
Publication date: 2022-05-17
Anticipated expiration: 2031-12-20

Abstract

The utility model discloses an intelligent quantitative detection auxiliary device for a mycotoxin quantum dot test strip. The test strip which is placed in the shell and sprayed with the mycotoxin antigens is excited through the first light source and the second light source to obtain emitted light, and impurity light is filtered through the light filtering component. Carry out the centre gripping through fixture to shooting the part fixedly, recycle and shoot the part and catch mycotoxin quantum dot test paper strip picture to carry out analysis to picture information, obtain the content result of mycotoxin, shortened check out time, improved the portability and the practicality that mycotoxin detected, realized many toxin mixed pollution ration instant detect, solved fluorescence quantitative technical problem.

Description

Intelligent quantitative detection auxiliary equipment for mycotoxin quantum dot test paper strip

Technical Field

The utility model relates to the technical field of mycotoxin detection, in particular to an intelligent quantitative detection auxiliary device for a mycotoxin quantum dot test strip.

Background

Food safety is a major problem related to the national people, and the food in China is easily polluted by a mixture of various mycotoxins, so that the life health and the economic trade safety of the nation are seriously threatened. Mycotoxins are secondary metabolites produced by fungi and are among the most dangerous food contaminants produced naturally, with about 45 million people worldwide at risk for long-term exposure to mycotoxins. More than 300 fungal toxins which are separated and identified at present, wherein aflatoxin B1(AFB1) is defined as a carcinogen by International cancer research Institute (IARC), can induce liver cancer, and has teratogenic and mutagenic effects. OTA has nephrotoxicity and can cause urinary cancer, and IARC has classified OTA into two carcinogens; ZEN has estrogenic effects and can cause poor semen quality and impaired reproductive function. In Asia and Africa areas, mycotoxin pollution is particularly serious, and acute or chronic diseases of human and livestock are easily caused, so that a great food safety problem is caused. According to research, it has been shown that, in the future, european crops will also be contaminated with more mycotoxins, most of which are mixed pollution, as the climate changes. Mycotoxins are naturally produced, and mixed pollution can occur in various links of grain planting, collection, storage, transportation and the like. Therefore, the establishment of a high-sensitivity intelligent instant detection technology for mycotoxin mixed pollution is an urgent need for guaranteeing the food quality safety of China and promoting the high-quality development of the food industry.

At present, the mycotoxin detection method mainly comprises a biological identification method, a chemical analysis method, a large-scale instrument analysis technology and the like. The biological identification method is a traditional mycotoxin identification method, mainly utilizes cells of organisms such as poultry, microorganisms and aquatic organisms which can be influenced by mycotoxins to detect whether the mycotoxins really exist or not, has poor specificity and low sensitivity, cannot quantify, and is usually only used as an assistant certificate of a chemical analysis method; the chemical analysis method mainly utilizes the thin layer chromatography technology to separate and analyze the mycotoxin and can realize the purification of the mycotoxin, the method has simple operation and low cost, which is written into the national standard once, but the method is gradually eliminated due to the large use of solvents and the risk of direct contact of operators with the mycotoxin; the large-scale instrument analysis technology is a mainstream mycotoxin analysis method at present, such as High Performance Liquid Chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC-MS/MS) and the like, and due to the characteristics of high sensitivity, accurate measurement result, good specificity and the like, the HPLC and the LC-MS/MS become standard methods for detecting mycotoxins in China (national standard GB 5009.22-2016) and European Union (European standardization Committee EN ISO 16050:2011) and other countries and regions in the world, are widely applied to dairy products, peanuts, feeds and Chinese medicinal materials, and have been reported to detect as many as 55 mycotoxins. However, fungi are easily affected by climate, soil and storage conditions, and the sample feeding, pretreatment and detection processes in the laboratory chromatographic mass spectrometry detection are long, so that the content of mycotoxin is changed, and the supervision and risk assessment of mycotoxin are not facilitated.

In order to meet the demand of market supervision on the real-time detection of agricultural product mycotoxin, the development of an intelligent real-time detection device with higher detection speed and better portability has important research and application values.

SUMMERY OF THE UTILITY MODEL

The utility model provides the auxiliary device for the intelligent quantitative detection of the mycotoxin quantum dot test strip, so that the technical effects of higher detection speed and better portability are realized.

The utility model provides an intelligent quantitative detection auxiliary device for a mycotoxin quantum dot test strip, which comprises: the device comprises a shell, a first light source, a second light source, a filtering component, a clamping mechanism for clamping the shooting component and a shading door; the first light source and the second light source are both arranged inside the shell; the first light source and the second light source face each other and are coated with mycotoxin antigens; the top of the shell is provided with a top opening; the light filtering component seals the top opening; the clamping mechanism is positioned above the top of the shell; a camera of the shooting component in the clamping mechanism faces the light filtering component; a side opening is arranged at the side part of the shell; the shading door is arranged at the opening of the side part.

Specifically, the light-shielding door is arranged at the side opening in a hinged or sliding connection manner.

Specifically, the first light source and the second light source are arranged in the shell at an included angle β; said beta is according to the formula

Determining; wherein s is a transverse distance between the first light source and the second light source, d is a transverse distance of the emitted light of the first light source or the second light source irradiating on the test strip region, and h is a vertical distance of the first light source or the second light source from the test strip region.

Specifically, the clamping mechanism includes: the cavity comprises a first L-shaped clamping plate, a second L-shaped clamping plate, an elastic component, a first bulge, a second bulge, a first elastic base, a second elastic base and a cavity body; the first L-shaped clamp plate and the second L-shaped clamp plate are respectively arranged on two sides of the cavity body; the horizontal part of the first L-shaped clamp plate is connected with the horizontal part of the second L-shaped clamp plate through the elastic part; the elastic component penetrates through the cavity body; a first opening and a second opening are respectively arranged at two ends of the upper wall of the cavity body; the first bulge is vertically arranged on the first elastic base, and the first elastic base is arranged on the horizontal part of the first L-shaped clamping plate; the second protrusion is vertically arranged on the second elastic base, and the second elastic base is arranged on the horizontal part of the second L-shaped clamping plate.

Specifically, a non-slip flexible material is provided on the inner walls of the first and second L-shaped cleats.

In particular, the anti-slip flexible material is rubber.

Specifically, the filter member is an optical filter.

One or more technical schemes provided by the utility model at least have the following technical effects or advantages:

the test paper strip which is placed in the shell and is sprayed with the mycotoxin antigens is excited through the first light source and the second light source to obtain emitted light, and impurity light is filtered out through the light filtering component. Carry out the centre gripping through fixture to shooting the part fixedly, recycle and shoot the part and catch mycotoxin quantum dot test paper strip picture to carry out analysis to picture information, obtain the content result of mycotoxin, shortened check out time, improved the portability and the practicality that mycotoxin detected, realized many toxin mixed pollution ration instant detect, solved fluorescence quantitative technical problem.

Drawings

Fig. 1 is a perspective view of an auxiliary device for intelligent quantitative detection of a mycotoxin quantum dot test strip provided by an embodiment of the utility model;

fig. 2 is a structural diagram of a geometric relationship between a first light source 2 and a second light source 3 in an auxiliary device for intelligent quantitative detection of mycotoxin quantum dot test paper provided in an embodiment of the present invention;

fig. 3 is a perspective view of a clamping mechanism 5 in an auxiliary device for intelligent quantitative detection of mycotoxin quantum dot test paper provided by an embodiment of the utility model;

fig. 4 is a front sectional view of a clamping mechanism 5 in an initial state in an auxiliary device for intelligent quantitative detection of mycotoxin quantum dot test paper provided in an embodiment of the present invention;

fig. 5 is a front sectional view of a clamping mechanism 5 in an auxiliary device for intelligent quantitative detection of mycotoxin quantum dot test paper strips according to an embodiment of the present invention in a use state;

the test paper strip comprises a shell, a first light source, a second light source, a 4-filtering component, a 5-clamping mechanism, a first L-shaped clamping plate, a second L-shaped clamping plate, an elastic component, a 9-first protrusion, a 10-second protrusion, a 11-first elastic base, a 12-second elastic base, a 13-cavity body, a 14-shading door, a 15-battery, a 16-power switch, a 17-test paper strip clamping groove, a 18-first opening and a 19-second opening, wherein the shell is arranged in the shell, and the first light source, the second light source, the 3-second light source, the 4-filtering component, the 5-clamping mechanism, the 6-first L-shaped clamping plate, the 7-second L-shaped clamping plate, the 8-elastic component, the 9-first protrusion, the 10-second protrusion, the 11-first elastic base, the 12-second elastic base, the 13-cavity body, the 14-shading door, the 15-battery, the 16-power switch, the 17-test paper strip clamping groove, the 18-first opening and the 19-second opening.

Detailed Description

The embodiment of the utility model provides the auxiliary device for the intelligent quantitative detection of the mycotoxin quantum dot test strip, so that the technical effects of higher detection speed and better portability are realized.

In order to achieve the technical effects, the technical scheme in the embodiment of the utility model has the following general idea:

For better understanding of the above technical solutions, the following detailed descriptions will be provided in conjunction with the drawings and the detailed description of the embodiments.

Referring to fig. 1, the intelligent quantitative detection auxiliary device for the mycotoxin quantum dot test strip provided by the embodiment of the utility model comprises: the device comprises a shell 1, a first light source 2, a second light source 3, a filter component 4, a clamping mechanism 5 for clamping the shooting component and a shading door 14; the first light source 2 and the second light source 3 are both arranged inside the housing 1; the first light source 2 and the second light source 3 are opposite to the test strip which is arranged inside the shell 1 and sprayed with mycotoxin antigens; the top of the shell 1 is provided with a top opening; the top opening is sealed by the filter component 4; the clamping mechanism 5 is positioned above the top of the shell 1; the camera of the shooting component in the clamping mechanism 5 faces the filter component 4; a side opening is arranged at the side part of the shell 1; the light-shielding door 14 is provided at the side opening. A battery 15 is arranged in the housing 1, and a power switch 16 is arranged on the battery 15, and can control the battery 15 and supply power to the first light source 2 and the second light source 3. The filter member 4 is used to filter out impurity light.

Specifically describing the arrangement of the light-shielding door 14, the light-shielding door 14 is arranged at the side opening by means of a hinge or a sliding connection.

In this embodiment, the test strip is disposed in a test strip slot 17 in the bottom of the housing 1. The filtering component 4 is a filter, and the shooting component is a mobile phone, a tablet personal computer and the like.

Describing the position relationship of the first light source 2 and the second light source 3 in the housing 1, referring to fig. 2, the first light source 2 and the second light source 3 are disposed in the housing 1 at an included angle β; beta according to the formula

Determining; where s is the lateral distance between the first light source 2 and the second light source 3, d is the lateral distance of the first light source 2 or the second light source 3 irradiated on the strip area, and h is the vertical distance of the first light source 2 or the second light source 3 from the strip area.

Specifically, first light source 2 and second light source 3 comprise two LED lamp pearls, under the prerequisite that the irradiation range of guaranteeing the light source can cover test paper strip detection area, for making the light source more concentrate, excitation light intensity is stronger, in this embodiment, sets for 45 with the light emission angle of LED lamp pearl. Beta is 115 degrees; h is more than or equal to 6.5cm, so that the camera can focus.

To explain the structure of the chucking mechanism 5 in detail, referring to fig. 3 to 5, the chucking mechanism 5 includes: the clamping device comprises a first L-shaped clamping plate 6, a second L-shaped clamping plate 7, an elastic component 8, a first bulge 9, a second bulge 10, a first elastic base 11, a second elastic base 12 and a cavity body 13; the first L-shaped clamp plate 6 and the second L-shaped clamp plate 7 are respectively arranged at two sides of the cavity body 13; the horizontal part of the first L-shaped splint 6 and the horizontal part of the second L-shaped splint 7 are connected by an elastic member 8; the elastic part 8 passes through the cavity body 13; a first opening 18 and a second opening 19 are respectively arranged at two ends of the upper wall of the cavity body 13; the first bulge 9 is vertically arranged on a first elastic base 11, and the first elastic base 11 is arranged on the horizontal part of the first L-shaped clamping plate 6; the second protrusion 10 is vertically disposed on a second elastic base 12, and the second elastic base 12 is disposed on the horizontal portion of the second L-shaped splint 7.

Before detection, the first L-shaped clamp plate 6 and the second L-shaped clamp plate 7 are pulled apart in opposite directions, namely the first protrusion 9 and the second protrusion 10 are pulled in opposite directions until the first protrusion and the second protrusion move to the positions below the first opening 18 and the second opening 19 respectively, and then the first elastic base 11 and the second elastic base 12 push the first protrusion 9 and the second protrusion 10 upwards respectively and penetrate through the first opening 18 and the second opening 19 respectively to be fixed. When the shooting component is placed on the upper wall of the cavity body 13, the first protrusion 9 and the second protrusion 10 are pressed downwards due to the self-gravity of the shooting component, the first elastic base 11 and the second elastic base 12 are compressed, and the first protrusion 9 and the second protrusion 10 retract into the cavity body 13 to be released from fixation. The first and second L-

shaped clamping plates

6 and 7 are moved toward each other by the contraction force of the elastic member 8 until the photographing part is clamped and its position is fixed. After the position of the shooting part is fixed, the shooting position is fixed, so that the stability and the precision of shooting are improved, and the detection error is reduced.

In order to firmly clamp the photographing part while avoiding abrasion to the side of the photographing part, an anti-slip flexible material is provided on the inner walls of the first and second L-

shaped clamping plates

6 and 7.

In this embodiment, the non-slip flexible material is rubber.

The process of analyzing the mycotoxin by the auxiliary device for the intelligent quantitative detection of the mycotoxin quantum dot test strip provided by the embodiment of the utility model is as follows:

the smartphone is placed in the fixture 5. The light-shielding door 14 is opened, the test strip sprayed with the mycotoxin antigen is placed in the test strip clamping groove 17 of the shell 1, and the light-shielding door 14 is closed. The power switch 16 of the built-in battery 15 is turned on, the first light source 2 and the second light source 3 are immediately turned on and project exciting light to the test strip, fluorescent substances pre-coated on the test strip emit fluorescence under the excitation of the exciting light, the image information of the fluorescent test strip is shot by the smart phone through the filtering component 4, and then the image of the fluorescent test strip is processed to obtain a mycotoxin result.

Example 1 (agricultural food)

Detection of aflatoxin and zearalenone in corn:

60g of corn kernels were weighed, 60mL of acetonitrile/water solution (80:20, v: v) was added, homogenized at 18000rpm for 2 minutes, and filtered using glass fiber filter paper. The filtrate was collected, and 1mL of the filtrate was added to 3mL of a sample dilution (PBS buffer containing 1% BSA, 1% sucrose, 2% tween-20, 0.5% PVPK-30, pH 7.4, 0.01M). And (3) putting 200 mu L of the mixed solution and 1 mu L of the quantum dot fluorescent probe into the enzyme-labeled micropore, inserting a mycotoxin quantum dot test strip, and incubating for 6min at 37 ℃. And taking out the test strip. And (3) inserting the test strip into a test strip clamping groove 17 of the shell 1, photographing by a mobile phone, and acquiring and converting picture data to obtain the contents of aflatoxin and zearalenone in the corn.

Example 2 (Life health)

Detection of aflatoxin and zearalenone in urine:

10mL of urine was filtered through a quantitative filter paper, and 1mL of the filtered urine was collected and added to 1mL of a sample dilution (PBS buffer containing 2% BSA, 2% sucrose, 4% tween-20, and 1% PVPK-30, pH 7.4, 0.02M). And (3) putting 200 mu L of the mixed solution and 1 mu L of the quantum dot fluorescent probe into the enzyme-labeled micropore, inserting a mycotoxin quantum dot test strip, and incubating for 6min at 37 ℃. And taking out the test strip. And (3) inserting the test strip into a test strip clamping groove 17 of the shell 1, photographing by a mobile phone, and acquiring and converting picture data to obtain the contents of aflatoxin and zearalenone in urine.

Technical effects

1. The test paper strip which is placed in the shell 1 and is sprayed with the mycotoxin antigens is excited by the first light source 2 and the second light source 3 to obtain emitted light, and impurity light is filtered by the filtering component 4. Carry out the centre gripping through fixture 5 to shooting the part and fix, recycle and shoot the part and catch mycotoxin quantum dot test paper strip picture to carry out analysis to picture information, obtain the content result of mycotoxin, shortened check out time, improved the portability and the practicality that mycotoxin detected, realized many toxin mixed pollution ration instant detection, solved fluorescence quantitative technical problem.

2. By arranging the first L-shaped clamping plate 6, the second L-shaped clamping plate 7, the elastic component 8, the first protrusion 9, the second protrusion 10, the first elastic base 11, the second elastic base 12 and the cavity body 13, the distance between the first L-shaped clamping plate 6 and the second L-shaped clamping plate 7 can be adjusted, so that the mycotoxin quantum dot test strip intelligent quantitative detection auxiliary equipment provided by the embodiment of the utility model can be suitable for shooting components with more sizes, and the application range is wider.

3. There is the flexible material of antiskid on the inner wall of first L type splint 6 and second L type splint 7, has realized the firm centre gripping to shooting the part, has avoided causing wearing and tearing to shooting the lateral part of part simultaneously.

While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the utility model.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the utility model. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. The utility model provides a mycotoxin quantum dot test paper strip intelligence quantitative determination auxiliary assembly which characterized in that includes: the device comprises a shell, a first light source, a second light source, a filtering component, a clamping mechanism for clamping the shooting component and a shading door; the first light source and the second light source are both arranged inside the shell; the first light source and the second light source face each other and are coated with mycotoxin antigens; the top of the shell is provided with a top opening; the light filtering component seals the top opening; the clamping mechanism is positioned above the top of the shell; a camera of the shooting component in the clamping mechanism faces the light filtering component; a side opening is arranged at the side part of the shell; the shading door is arranged at the opening of the side part.

2. The apparatus of claim 1, wherein the shutter door is disposed at the side opening by means of a hinged or sliding connection.

3. The apparatus of claim 1, wherein the first light source and the second light source are disposed in the housing at an included angle β; said beta is according to the formula

4. The apparatus of claim 1, wherein the clamping mechanism comprises: the cavity comprises a first L-shaped clamping plate, a second L-shaped clamping plate, an elastic component, a first bulge, a second bulge, a first elastic base, a second elastic base and a cavity body; the first L-shaped clamp plate and the second L-shaped clamp plate are respectively arranged on two sides of the cavity body; the horizontal part of the first L-shaped clamp plate is connected with the horizontal part of the second L-shaped clamp plate through the elastic part; the elastic component penetrates through the cavity body; a first opening and a second opening are respectively arranged at two ends of the upper wall of the cavity body; the first bulge is vertically arranged on the first elastic base, and the first elastic base is arranged on the horizontal part of the first L-shaped clamping plate; the second protrusion is vertically arranged on the second elastic base, and the second elastic base is arranged on the horizontal part of the second L-shaped clamping plate.

5. The apparatus of claim 4, wherein a non-slip flexible material is on the inner walls of the first L-clamp and the second L-clamp.

6. The apparatus of claim 5, wherein the non-slip flexible material is rubber.

7. The apparatus of any of claims 1-6, wherein the filtering component is a filter.